PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 22393877-6 2012 RESULTS: We found that E(2)-mediated cellular activation signaling through extracellular signal-regulated kinase (ERK)-1/2. Estradiol 23-27 mitogen-activated protein kinase 3 Homo sapiens 75-122 22493348-3 2012 RESULTS: Compared to the control, 1 muM genistein plus 1 nM 17beta-estradiol significantly increased apoptosis, and the BAX/BCL-2 ratio, with a concomitant decrease in ERK1/2 phosphorylation. Estradiol 60-76 mitogen-activated protein kinase 3 Homo sapiens 168-174 22366173-3 2012 17beta-Estradiol and the selective ERalpha agonist PPT induced an increase of ERalpha expression in the plasma membrane and activated the PKCalpha/ERK 1/2 pathway in a time-course not compatible with genomic actions, thus supporting the notion of membrane-initiated effects. Estradiol 0-16 mitogen-activated protein kinase 3 Homo sapiens 147-154 22295107-5 2012 The treatment with estradiol (E2), anti-estrogenic agents 4-hydroxytamoxifen and ICI 182780, ERbeta specific ligand DPN and GPR30 agonist G1 led to a rapid activation of p-ERK1/2, suggesting the involvement of ERalpha36, ERbeta and GPR30 in the non-genomic signaling pathway in these cells. Estradiol 19-28 mitogen-activated protein kinase 3 Homo sapiens 172-178 22329816-1 2012 OBJECTIVE: The current study was undertaken to determine the involvement of cAMP/PKA and MAPK-mediated signalling pathways in the regulation of cell proliferation by hydroxylated metabolites of 17beta-estradiol (E2). Estradiol 194-210 mitogen-activated protein kinase 3 Homo sapiens 89-93 22329816-11 2012 Phospho-ERK1/2 protein expression in cells stimulated with E2, 2-OH-E2 and 4-OH-E2 was not significantly different from the control. Estradiol 59-61 mitogen-activated protein kinase 3 Homo sapiens 8-14 21720253-1 2011 OBJECTIVE: The objectives of the study were to evaluate the role of mitogen-activated protein kinase (MAPK) signaling in normal, hyperplastic, and neoplastic endometrium in relation to estrogen receptor (ER) status and to investigate whether 17beta-estradiol (E2) and tamoxifen (TAM) mediate the proliferation and apoptosis of endometrial cancer cells through the MAPK pathway. Estradiol 242-258 mitogen-activated protein kinase 3 Homo sapiens 102-106 21720253-3 2011 The expression levels of p-ERK1/2 and t-ERK1/2 in RL95-2 and KLE after stimulation by E2, progesterone (P), and TAM were detected by Western blotting. Estradiol 86-88 mitogen-activated protein kinase 3 Homo sapiens 27-33 21720253-7 2011 17beta-Estradiol increased ERK1/2 phosphorylation, and TAM decreased ERK1/2 phosphorylation in endometrial cancer cell lines within 30 minutes (P < 0.05). Estradiol 0-16 mitogen-activated protein kinase 3 Homo sapiens 27-33 21720253-3 2011 The expression levels of p-ERK1/2 and t-ERK1/2 in RL95-2 and KLE after stimulation by E2, progesterone (P), and TAM were detected by Western blotting. Estradiol 86-88 mitogen-activated protein kinase 3 Homo sapiens 40-46 21803156-5 2011 Our current study demonstrates that 17beta-estradiol activates ERK1/2 and Akt at the early stage and induces ERalpha and survivin mRNA at the late stage to modulate its protection via the suppression of caspase-3 activation in PCZ. Estradiol 36-52 mitogen-activated protein kinase 3 Homo sapiens 63-69 21278053-9 2011 Lindane did not activate these pathways, but it inhibited estradiol-mediated Akt and ERK1/2 activation. Estradiol 58-67 mitogen-activated protein kinase 3 Homo sapiens 85-91 20110355-5 2010 Inhibiting these transporters also blocked E(2)-induced activation of ERK1/2, indicating that E(2) activates ERK via downstream signaling of S1P. Estradiol 43-47 mitogen-activated protein kinase 3 Homo sapiens 70-76 20446020-0 2010 17beta-estradiol inhibits prostaglandin E2-induced COX-2 expressions and cell migration by suppressing Akt and ERK1/2 signaling pathways in human LoVo colon cancer cells. Estradiol 0-16 mitogen-activated protein kinase 3 Homo sapiens 111-117 20446020-5 2010 We further observed that 17beta-estradiol treatment inhibits PGE2-induced COX-2 expression and cellular motility via suppressing activation of Akt and ERK1/2 in human LoVo cancer cells. Estradiol 25-41 mitogen-activated protein kinase 3 Homo sapiens 151-157 20110355-5 2010 Inhibiting these transporters also blocked E(2)-induced activation of ERK1/2, indicating that E(2) activates ERK via downstream signaling of S1P. Estradiol 94-98 mitogen-activated protein kinase 3 Homo sapiens 70-76 20197310-5 2010 Both 17beta-estradiol (E2) and G1, a compound reported to be a selective GPR30 agonist, increased the phosphorylation levels of the MAPK/ERK1/2 in SK-BR-3 cells, which could be blocked by an anti-ER-alpha36-specific antibody against its ligand-binding domain. Estradiol 5-21 mitogen-activated protein kinase 3 Homo sapiens 132-136 20197310-5 2010 Both 17beta-estradiol (E2) and G1, a compound reported to be a selective GPR30 agonist, increased the phosphorylation levels of the MAPK/ERK1/2 in SK-BR-3 cells, which could be blocked by an anti-ER-alpha36-specific antibody against its ligand-binding domain. Estradiol 5-21 mitogen-activated protein kinase 3 Homo sapiens 137-143 19041697-9 2009 The sustained ERK1/2 phosphorylation may contribute to the Cd-induced enhancement of DNA synthesis and pS2 mRNA in mixture with low-concentration E2. Estradiol 146-148 mitogen-activated protein kinase 3 Homo sapiens 14-20 19556604-7 2010 Likewise, higher rapid, non-genomic E(2)-induced extracellular signal-regulated kinase 1/2 activation was detected in H1793 compared with A549 cells, linking extracellular signal-regulated kinase activation to increased P-ser118-ER alpha. Estradiol 36-40 mitogen-activated protein kinase 3 Homo sapiens 49-90 19433249-5 2009 Activation of ERalpha by 17beta-estradiol (E2) and the environmental estrogen bisphenol-A (BPA) promotes an increase of insulin biosynthesis through a non-classical estrogen-activated pathway that involves phosphorylation of ERK1/2. Estradiol 25-41 mitogen-activated protein kinase 3 Homo sapiens 225-231 18437557-8 2009 As a consequence, D327N caused a larger induction of the second messenger cAMP and a deeper inhibition of the estradiol-induced Erk (1/2) phosphorylation. Estradiol 110-119 mitogen-activated protein kinase 3 Homo sapiens 128-136 18852162-8 2009 These findings suggest that E(2)-induced proliferation of endometrial carcinoma cells is mediated by the MAPK3/1 pathway via autocrine stimulation of IGF1. Estradiol 28-32 mitogen-activated protein kinase 3 Homo sapiens 105-110 17959712-2 2008 Stromal cell-derived factor-1 (SDF-1) enhances extracellular signal-regulated kinases 1 and 2 (ERK1/2) activity via the transactivation of EGFR and 17beta-estradiol (E2) induces SDF-1 production to exert autocrine proliferative effects. Estradiol 148-164 mitogen-activated protein kinase 3 Homo sapiens 95-101 18296501-8 2008 Depletion of endogenous ER alpha, not ERbeta, by siRNA attenuated resveratrol- and E(2)-induced ERK1/2, Src, and eNOS phosphorylation. Estradiol 83-87 mitogen-activated protein kinase 3 Homo sapiens 96-102 18539708-9 2008 However, comparison between the amount of phosphorylated ERK1/2 in SLE T cells from the same patients cultured without and with oestradiol showed a significant oestrogen-dependent suppression (P=0.48) of ERK1/2 in patients with inactive/mild systemic lupus erythematosus disease activity index (SLEDAI) (0-2) compared with patients with moderate (4-6) or active (8-12) SLEDAI scores. Estradiol 128-138 mitogen-activated protein kinase 3 Homo sapiens 204-210 18539708-10 2008 These results suggest that the suppression of MAPK through ERK1/2 phosphorylation is sensitive to oestradiol in patients with inactive or mild disease, but the sensitivity is not maintained when disease activity increases. Estradiol 98-108 mitogen-activated protein kinase 3 Homo sapiens 59-65 18275979-10 2008 It is concluded that Cd, like estradiol, can cause rapid activation of ERK1/2 and AKT and that these signaling events are mediated by possible interaction with membrane ER alpha and GPR30, but not ER beta. Estradiol 30-39 mitogen-activated protein kinase 3 Homo sapiens 71-77 18039775-3 2008 17beta-Estradiol (E2) conjugated to BSA was able to stimulate human testicular seminoma cell proliferation by triggering a rapid, nongenomic, membrane-mediated activation of ERK1/2 and cAMP-dependent protein kinase A (PKA). Estradiol 0-16 mitogen-activated protein kinase 3 Homo sapiens 174-180 18780779-12 2008 Estradiol rapidly activated MAPK phosphorylation including extracellular signal-regulated kinase 1/2, p38, and stress-activated protein kinase/c-Jun NH2-terminal kinase pathways and BMP6, BMP7, and activin preferentially inhibited estradiol-induced p38 phosphorylation. Estradiol 0-9 mitogen-activated protein kinase 3 Homo sapiens 28-32 18780779-12 2008 Estradiol rapidly activated MAPK phosphorylation including extracellular signal-regulated kinase 1/2, p38, and stress-activated protein kinase/c-Jun NH2-terminal kinase pathways and BMP6, BMP7, and activin preferentially inhibited estradiol-induced p38 phosphorylation. Estradiol 0-9 mitogen-activated protein kinase 3 Homo sapiens 59-100 18780779-12 2008 Estradiol rapidly activated MAPK phosphorylation including extracellular signal-regulated kinase 1/2, p38, and stress-activated protein kinase/c-Jun NH2-terminal kinase pathways and BMP6, BMP7, and activin preferentially inhibited estradiol-induced p38 phosphorylation. Estradiol 231-240 mitogen-activated protein kinase 3 Homo sapiens 28-32 18289621-7 2008 Estradiol, estradiol-BSA and insulin stimuli increased the PKC epsilon, phosphorylated ERK 1/2 and Pit-1 expression, although combined treatments with estradiol/insulin or estradiol-BSA/insulin induced a significant reduction in these levels, in close correlation with the decrease of lactotroph cell proliferation. Estradiol 0-9 mitogen-activated protein kinase 3 Homo sapiens 87-94 17363451-10 2007 17beta-Estradiol was shown to exert a different kind of temporal pattern of ERK nuclear localization in comparison with Tam. Estradiol 0-16 mitogen-activated protein kinase 3 Homo sapiens 76-79 17138646-5 2007 Here we show that estradiol acts via the classical estrogen receptors, the IGF-I receptor, and the ERK/MAPK signaling cascade to protect CA1 neurons in ovariectomized female rats and gerbils. Estradiol 18-27 mitogen-activated protein kinase 3 Homo sapiens 103-107 17350964-0 2007 PI3K/Akt and ERK1/2 signalling pathways are involved in endometrial cell migration induced by 17beta-estradiol and growth factors. Estradiol 94-110 mitogen-activated protein kinase 3 Homo sapiens 13-19 17350964-7 2007 Similarly, 17beta-estradiol (10(-6)-10(-8) M) could enhance both constitutive and PDGF-induced migration of the cells and their rapid treatment with the hormone significantly increased phosphorylation of ERK1/2 and Akt. Estradiol 11-27 mitogen-activated protein kinase 3 Homo sapiens 204-210 17138646-7 2007 Estradiol treatment increases basal phosphorylation of both ERK1 and ERK2 in hippocampal CA1 and prevents ischemia-induced dephosphorylation and inactivation of ERK1 and CREB, down-regulation of Bcl-2 and activation of the caspase death cascade. Estradiol 0-9 mitogen-activated protein kinase 3 Homo sapiens 60-64 17138646-7 2007 Estradiol treatment increases basal phosphorylation of both ERK1 and ERK2 in hippocampal CA1 and prevents ischemia-induced dephosphorylation and inactivation of ERK1 and CREB, down-regulation of Bcl-2 and activation of the caspase death cascade. Estradiol 0-9 mitogen-activated protein kinase 3 Homo sapiens 161-165 17138646-9 2007 These findings support a model whereby estradiol acts via the classical estrogen receptors and IGF-I receptors, which converge on activation of ERK/MAPK signaling and CREB to promote neuronal survival in the face of global ischemia. Estradiol 39-48 mitogen-activated protein kinase 3 Homo sapiens 148-152 16651411-4 2006 Although phosphorylated extracellular signal-regulated kinase 1/2 levels in these cells cultured under estradiol deplete and replete conditions displayed no change, a significant induction was observed after TPA treatment. Estradiol 103-112 mitogen-activated protein kinase 3 Homo sapiens 24-65 16936263-6 2006 These effects of 17beta-estradiol and IGF-1 were associated with enhanced protein expression of ER-alpha, phosphorylated (p)-ERK1/2 and pAKT but with decreased expression of ER-beta. Estradiol 17-33 mitogen-activated protein kinase 3 Homo sapiens 125-131 16113101-5 2005 We have shown that long-term culture of MCF7 cells in medium depleted of oestrogen (LTED) results in hypersensitivity to oestradiol (E2) coinciding with elevated levels of both ERalpha phosphorylated on Ser(118) and ERK1/ERK2. Estradiol 121-131 mitogen-activated protein kinase 3 Homo sapiens 216-220 16050986-0 2005 17beta-estradiol stimulates MAPK signaling pathway in human lens epithelial cell cultures preventing collapse of mitochondrial membrane potential during acute oxidative stress. Estradiol 0-16 mitogen-activated protein kinase 3 Homo sapiens 28-32 16019207-1 2005 While some studies report that estradiol (E2) activates extracellular-signal regulated kinase (Erk1/2) in MCF-7 breast cancer cells, others report E2 does not activate this signaling pathway. Estradiol 31-40 mitogen-activated protein kinase 3 Homo sapiens 95-101 15642162-9 2005 These two separated cell subpopulations also exhibited different kinetics of ERK1/2 activation with 1 pmol/l 17beta-estradiol (E2), as well as different patterns of E2 dose-dependent responsiveness. Estradiol 109-125 mitogen-activated protein kinase 3 Homo sapiens 77-83 15924567-0 2005 17beta-oestradiol up-regulates longevity-related, antioxidant enzyme expression via the ERK1 and ERK2[MAPK]/NFkappaB cascade. Estradiol 0-17 mitogen-activated protein kinase 3 Homo sapiens 88-92 15924567-5 2005 Oestradiol increases MAP kinase (MAPK) activation as indicated by ERK1 and ERK2 phosphorylation in MCF-7 cells, which in turn activates the nuclear factor kappa B (NFkappaB) signalling pathways as indicated by an increase in the p50 subunit of NFkappaB in nuclear extracts. Estradiol 0-10 mitogen-activated protein kinase 3 Homo sapiens 66-70 15664449-8 2005 Moreover, SHBG prevented the estradiol-induced phosphorylation of Erk-1/-2, whereas it had no effect on estradiol-induced transcription. Estradiol 29-38 mitogen-activated protein kinase 3 Homo sapiens 66-74 15642162-9 2005 These two separated cell subpopulations also exhibited different kinetics of ERK1/2 activation with 1 pmol/l 17beta-estradiol (E2), as well as different patterns of E2 dose-dependent responsiveness. Estradiol 127-129 mitogen-activated protein kinase 3 Homo sapiens 77-83 12888920-0 2003 17beta-estradiol-induced activation of ERK1/2 through endogenous androgen receptor-estradiol receptor alpha-Src complex in human prostate cells. Estradiol 0-16 mitogen-activated protein kinase 3 Homo sapiens 39-45 15634404-2 2004 METHODS: Epithelial growth factor (EGF) and different concentration of estradiol to induce the expression of phosphospecific ERK1/2 (pERK1/2) in MCF-7 cell line was used and the expression of pERK1/2 with western-blotting was detected. Estradiol 71-80 mitogen-activated protein kinase 3 Homo sapiens 125-131 15188005-3 2004 Treatment of MCF-7 cells with resveratrol in the presence of 17beta-oestradiol (E(2)) further enhanced MAPK activation, but E(2) blocked resveratrol-induced apoptosis, as measured by nucleosome ELISA and DNA fragmentation assays. Estradiol 61-78 mitogen-activated protein kinase 3 Homo sapiens 103-107 12881510-7 2003 In contrast, expression of SphKG82D, a dominant-negative mutant SphK, profoundly inhibited the E2-mediated Ca2+ mobilization, Erk1/2 activity and neoplastic cell growth. Estradiol 95-97 mitogen-activated protein kinase 3 Homo sapiens 126-132 12888920-4 2003 The activation of ERK1/2 through 17beta-estradiol triggered simultaneous association of endogenous androgen receptor, estrogen receptor alpha and Src. Estradiol 33-49 mitogen-activated protein kinase 3 Homo sapiens 18-24 15292328-4 2004 Norepinephrine, epinephrine, isoproterenol, and OR486 (COMT inhibitor) abrogated the inhibitory effects of estradiol on SMC growth and ERK1/2 phosphorylation. Estradiol 107-116 mitogen-activated protein kinase 3 Homo sapiens 135-141 15184047-5 2004 The ability of estrogen to rapidly activate MAPK signaling was tested; low physiological concentrations of E(2) stimulated ERK1/2 phosphorylation and nuclear translocation within 15min of exposure. Estradiol 107-111 mitogen-activated protein kinase 3 Homo sapiens 44-48 15184047-5 2004 The ability of estrogen to rapidly activate MAPK signaling was tested; low physiological concentrations of E(2) stimulated ERK1/2 phosphorylation and nuclear translocation within 15min of exposure. Estradiol 107-111 mitogen-activated protein kinase 3 Homo sapiens 123-129 15001646-9 2004 Upon estradiol exposure, we observed an enhanced phosphorylation of the proteins involved in the phosphatidylinositol-3-OH kinase (PI3K)/Akt pathway like PDK1, Akt, GSK-3, Bcl-2, together with ERK1/2, which was also involved in cell survival signals. Estradiol 5-14 mitogen-activated protein kinase 3 Homo sapiens 193-199 12888920-2 2003 17beta-estradiol (E2) caused a rapid and transient activation of MAPK (ERK1/2) within 5 min. Estradiol 0-16 mitogen-activated protein kinase 3 Homo sapiens 65-69 12888920-2 2003 17beta-estradiol (E2) caused a rapid and transient activation of MAPK (ERK1/2) within 5 min. Estradiol 0-16 mitogen-activated protein kinase 3 Homo sapiens 71-77 11735115-0 2001 Role of ERK1/2 in the differential synthesis of progesterone and estradiol by granulosa cells. Estradiol 65-74 mitogen-activated protein kinase 3 Homo sapiens 8-14 12763221-1 2003 Cellular response to oestradiol stimuli is mediated both by oestrogen receptor (ER) binding to oestrogen response elements (EREs) and by non-nuclear actions like activation of mitogen-activated protein kinase (MAPK) signal transduction. Estradiol 21-31 mitogen-activated protein kinase 3 Homo sapiens 210-214 11960991-0 2002 Mechanism of 17-beta-estradiol-induced Erk1/2 activation in breast cancer cells. Estradiol 13-30 mitogen-activated protein kinase 3 Homo sapiens 39-45 12763221-6 2003 We were able to demonstrate that oestradiol counteracted the inhibitory effects of trastuzumab by rapid phosphorylation of ERK-1/2, a kinase downstream of the HER2 receptor. Estradiol 33-43 mitogen-activated protein kinase 3 Homo sapiens 123-130 11978177-6 2002 Active Akt, in turn, prevented E(2) from activating p42/44 extracellular signal-regulated kinases (ERK 1/2). Estradiol 31-35 mitogen-activated protein kinase 3 Homo sapiens 99-106 11735115-3 2001 Here, we provide evidence that two members of the mitogen-activated protein kinase family, extracellular signal-regulated kinase-1 and -2 (ERK1/2) can differentially regulate FSH-stimulated estradiol and progesterone production. Estradiol 190-199 mitogen-activated protein kinase 3 Homo sapiens 139-145 11735115-8 2001 Collectively, this study clearly identified ERK1/2 as the first intracellular signaling molecules that differentially regulate FSH-induced progesterone and estradiol synthesis in GCs. Estradiol 156-165 mitogen-activated protein kinase 3 Homo sapiens 44-50 11488923-4 2001 Here, we show: (a) that physiological concentrations of E2 very rapidly cause an increased release of secreted nonamyloidogenic APP (sAPPalpha) in mouse hippocampal HT22 and human neuroblastoma SK-N-MC cells; and (b) that this effect is mediated through E2 via the phosphorylation of extracellular-regulated kinase 1 and 2 (ERK1/2), prominent members of the mitogen-activated protein kinase (MAPK) pathway. Estradiol 56-58 mitogen-activated protein kinase 3 Homo sapiens 324-330 10714766-5 2000 Phosphatidylinositol 3-kinase inhibitors, LY294002 and wortmannin, as well as inhibitors of MAPK kinase-1, PD098059 and U0126, decreased the fraction of cells entering DNA synthesis after treatment with 17beta-estradiol. Estradiol 203-219 mitogen-activated protein kinase 3 Homo sapiens 92-96 11043579-3 2000 We show that 17beta-estradiol activates Erk-1/-2 not only in MCF-7 cells, which express both estrogen receptor alpha (ER alpha) and ER beta, but also in SKBR3 breast cancer cells, which fail to express either receptor. Estradiol 13-29 mitogen-activated protein kinase 3 Homo sapiens 40-48 10328986-4 1999 17beta-Estradiol elicits rapid (within 5-15 min) and sustained (at least 2 h) tyrosine phosphorylation and activation of the MAP kinases, extracellular-signal regulated kinase (ERK)1, and ERK2, which is successfully inhibited by the MAP kinase/ERK kinase 1 inhibitor PD98059, but not by the estrogen receptor (ER) antagonist ICI 182,780 and also does not appear to result from estradiol-induced activation of trk. Estradiol 0-16 mitogen-activated protein kinase 3 Homo sapiens 138-182 10328986-4 1999 17beta-Estradiol elicits rapid (within 5-15 min) and sustained (at least 2 h) tyrosine phosphorylation and activation of the MAP kinases, extracellular-signal regulated kinase (ERK)1, and ERK2, which is successfully inhibited by the MAP kinase/ERK kinase 1 inhibitor PD98059, but not by the estrogen receptor (ER) antagonist ICI 182,780 and also does not appear to result from estradiol-induced activation of trk. Estradiol 377-386 mitogen-activated protein kinase 3 Homo sapiens 138-182 34478807-7 2022 Dual inhibition of AKT and ERK1/2 pathways suppresses the production of proinflammatory cytokines, decreases E2 biosynthesis and signaling, and restores progesterone receptor-B signaling components in the epithelial and stromal cells of the endometrium in a cell-specific manner. Estradiol 109-111 mitogen-activated protein kinase 3 Homo sapiens 27-33 9952396-4 1999 Estradiol elicited rapid (within 5-15 min) tyrosine phosphorylation/activation of the mitogen-activated protein (MAP) kinases, ERK1 and ERK2, that persisted for at least 2 hr. Estradiol 0-9 mitogen-activated protein kinase 3 Homo sapiens 127-131 9714091-6 1998 17Beta-estradiol (E2) stimulated a delayed (3 hours) 5- to 7-fold induction of ERK1/2 activity requiring activation of ER and new transcription/translation. Estradiol 0-16 mitogen-activated protein kinase 3 Homo sapiens 79-85 9714091-6 1998 17Beta-estradiol (E2) stimulated a delayed (3 hours) 5- to 7-fold induction of ERK1/2 activity requiring activation of ER and new transcription/translation. Estradiol 18-20 mitogen-activated protein kinase 3 Homo sapiens 79-85 9275096-2 1997 We show here, that in the human neuroblastoma cell line SK-N-SH, the membrane impermeable conjugated 17beta-estradiol (E2BSA) activates mitogen activated protein kinase kinase (MAPKK or MEK) and induces the phosphorylation and activation of both ERK-1 and ERK-2 (mitogen activated protein kinase or MAPK). Estradiol 101-117 mitogen-activated protein kinase 3 Homo sapiens 246-251 9275096-2 1997 We show here, that in the human neuroblastoma cell line SK-N-SH, the membrane impermeable conjugated 17beta-estradiol (E2BSA) activates mitogen activated protein kinase kinase (MAPKK or MEK) and induces the phosphorylation and activation of both ERK-1 and ERK-2 (mitogen activated protein kinase or MAPK). Estradiol 101-117 mitogen-activated protein kinase 3 Homo sapiens 177-181 8813150-7 1996 Estradiol treatment of subconfluent Caco-2 cells rapidly and reversibly stimulates four important intermediates in a signal transduction pathway that is known to trigger cell proliferation: two members of the large family of c-src-related tyrosine kinases, c-src and c-yes, and two serine/threonine kinases, the mitogen-activated protein (MAP) kinases, erk-1 and erk-2. Estradiol 0-9 mitogen-activated protein kinase 3 Homo sapiens 353-358 34821461-5 2022 Interestingly, E2 at environmental concentrations (0.1-10 nM) could activate the expression of both ERalpha36 and GPR30, and then stimulate the phosphorylation of ERK1/2 and Akt, resulting in cell growth promotion. Estradiol 15-17 mitogen-activated protein kinase 3 Homo sapiens 163-169 9582378-9 1998 The data suggest that a kinase other than p42/p44 MAPK is involved in the estradiol-induced Ser118 phosphorylation. Estradiol 74-83 mitogen-activated protein kinase 3 Homo sapiens 46-54 8635462-2 1996 Under conditions of estradiol-dependent growth, estradiol treatment of human mammary cancer MCF-7 cells triggers rapid and transient activation of the mitogen-activated (MAP) kinases, erk-1 and erk-2, increases the active form of p21ras, tyrosine phosphorylation of Shc and p190 protein and induces association of p190 to p21ras-GAP. Estradiol 20-29 mitogen-activated protein kinase 3 Homo sapiens 184-189 8635462-2 1996 Under conditions of estradiol-dependent growth, estradiol treatment of human mammary cancer MCF-7 cells triggers rapid and transient activation of the mitogen-activated (MAP) kinases, erk-1 and erk-2, increases the active form of p21ras, tyrosine phosphorylation of Shc and p190 protein and induces association of p190 to p21ras-GAP. Estradiol 48-57 mitogen-activated protein kinase 3 Homo sapiens 184-189 34877243-3 2021 Different studies show that down regulation of estrogen receptor beta (ERbeta) associates with GPR30 over-expression both in human testicular carcinoma in situ (CIS) and seminomas and that the mitogenic role exerted by 17beta-oestradiol induces the activation of extracellular signal-regulated kinase 1/2 (ERK1/2) through GPR30. Estradiol 219-236 mitogen-activated protein kinase 3 Homo sapiens 306-312 34571396-6 2021 In addition, we found that E2 significantly upregulated the mRNA levels of epidermal growth (EGF)-like factors in goat cumulus cells through GPER, and activated the downstream EGF receptor (EGFR) and extracellular signal-regulated kinase 1/2 (ERK1/2) signaling pathways. Estradiol 27-29 mitogen-activated protein kinase 3 Homo sapiens 243-249 34571396-7 2021 Both AG1478 (EGFR inhibitor) and U0126 (ERK1/2 inhibitor) abolished the inhibitory effect of E2 on the protein expression of NPR2. Estradiol 93-95 mitogen-activated protein kinase 3 Homo sapiens 40-46 34763099-11 2022 c-Src and p44/42 MAPK(ERK1/2) signaling pathways are required for E2"s induction on Smad1 phosphorylation. Estradiol 66-68 mitogen-activated protein kinase 3 Homo sapiens 22-28 34763099-13 2022 E2 promoted tube formation of vascular endothelial cells through c-Src/ERK1/2/Smad1 signaling pathway. Estradiol 0-2 mitogen-activated protein kinase 3 Homo sapiens 71-77 34763099-15 2022 In conclusion, E2 promotes proliferation, migration and tube formation of HUVECs through c-Src/ERK1/2/Smad1 signaling pathway. Estradiol 15-17 mitogen-activated protein kinase 3 Homo sapiens 95-101 31680862-8 2019 Estradiol treatment increased the level of MAP1A through the phosphorylation of ERK1/2 and increased the level of hemoglobin alpha through the phosphorylation of AKT. Estradiol 0-9 mitogen-activated protein kinase 3 Homo sapiens 80-86 35055130-10 2022 The obtained results indicate that chemerin affected P4 and E2 synthesis through the Erk1/2 and Akt signalling pathways. Estradiol 60-62 mitogen-activated protein kinase 3 Homo sapiens 85-91 33574796-5 2020 In human seminoma cell line, while 17beta-estradiol (E2) inhibits in vitro cell proliferation through an ERbeta-dependent mechanism, an impermeable E2 conjugate (E2 coupled to BSA), in vitro cell proliferation is stimulated by activating ERK1/2 and protein kinase A through a membrane GPCR that we further identified as GPER/GPR30. Estradiol 35-51 mitogen-activated protein kinase 3 Homo sapiens 238-244 33574796-5 2020 In human seminoma cell line, while 17beta-estradiol (E2) inhibits in vitro cell proliferation through an ERbeta-dependent mechanism, an impermeable E2 conjugate (E2 coupled to BSA), in vitro cell proliferation is stimulated by activating ERK1/2 and protein kinase A through a membrane GPCR that we further identified as GPER/GPR30. Estradiol 53-55 mitogen-activated protein kinase 3 Homo sapiens 238-244 32487604-2 2020 The Gs-coupled seven-transmembrane receptor GPR30 is known as both a CCL18 and an estrogen receptor; its activation by estradiol leads to a transactivation of membrane-tethered pro-heparin-binding EGF-like growth factor and the MAPK/ERK pathway. Estradiol 119-128 mitogen-activated protein kinase 3 Homo sapiens 228-232 33512728-0 2021 TGF-beta1 stimulates aromatase expression and estradiol production through SMAD2 and ERK1/2 signaling pathways in human granulosa-lutein cells. Estradiol 46-55 mitogen-activated protein kinase 3 Homo sapiens 85-91 32827543-10 2020 The phosphorylation of ERK1/2 but not AKT was altered by over-expressed or knocking out of ERalpha with or without the addition of E2 and LTZ. Estradiol 131-133 mitogen-activated protein kinase 3 Homo sapiens 23-29 31623342-9 2019 Treatment with the ER antagonist, ICI-182,780 (1 muM), completely blocked the effects of S-equol and 17beta-estradiol on cell viability, ERalpha, and ERK1/2 after Abeta (25-35) exposure. Estradiol 101-117 mitogen-activated protein kinase 3 Homo sapiens 150-156 30660702-5 2019 17beta-estradiol (E2), ERalpha-selective agonist PPT and ERbeta-selective agonist DPN induced a rapid increase on ERK1/2 phosphorylation. Estradiol 0-16 mitogen-activated protein kinase 3 Homo sapiens 114-120 30660702-5 2019 17beta-estradiol (E2), ERalpha-selective agonist PPT and ERbeta-selective agonist DPN induced a rapid increase on ERK1/2 phosphorylation. Estradiol 18-20 mitogen-activated protein kinase 3 Homo sapiens 114-120 30660702-6 2019 E2-induced ERK1/2 activation was partially inhibited when cells were pretreated with ERalpha- or ERbeta-selective antagonists, and blocked by simultaneous pretreatment with both antagonists, suggesting ERalpha/beta heterodimers formation. Estradiol 0-2 mitogen-activated protein kinase 3 Homo sapiens 11-17 26945908-12 2016 17beta-estradiol inhibits IL8-up-regulated Src downstream target proteins including p-Cas, p-paxillin, p-ERK1/2, p-JNK1/2, MMP9, tPA and uPA. Estradiol 0-16 mitogen-activated protein kinase 3 Homo sapiens 105-111 30445932-2 2018 The present study focuses on identifying the oncogenic potential and clinical significance of BKCa in endometrial adenocarcinoma, as well as exploring the mechanistic relevance by 17beta -estradiol (E2) inducing aberrant activation of MEK1/2 and ERK1/2 via BKCa. Estradiol 180-197 mitogen-activated protein kinase 3 Homo sapiens 246-252 28498246-10 2017 While MEK inhibition blocked estradiol-stimulated phosphorylation of ERK1/2 and p90RSK in wild-type cells, phospho-ERK1/2 and phospho-p90RSK were substantially increased in KRas mutants. Estradiol 29-38 mitogen-activated protein kinase 3 Homo sapiens 69-75 28498246-10 2017 While MEK inhibition blocked estradiol-stimulated phosphorylation of ERK1/2 and p90RSK in wild-type cells, phospho-ERK1/2 and phospho-p90RSK were substantially increased in KRas mutants. Estradiol 29-38 mitogen-activated protein kinase 3 Homo sapiens 115-121 26030000-4 2015 Here, we demonstrate that in SkBr3 breast cancer and HepG2 hepatocarcinoma cells, 17beta-estradiol (E2) and the selective GPER ligand G-1 induce miR144 expression through GPER and the involvement of the PI3K/ERK1/2/Elk1 transduction pathway. Estradiol 82-98 mitogen-activated protein kinase 3 Homo sapiens 208-214 26824324-0 2016 Metformin inhibits 17beta-estradiol-induced epithelial-to-mesenchymal transition via betaKlotho-related ERK1/2 signaling and AMPKalpha signaling in endometrial adenocarcinoma cells. Estradiol 19-35 mitogen-activated protein kinase 3 Homo sapiens 104-110 26824324-6 2016 Decreased expression of betaKlotho was noted in human endometrial adenocarcinomas, and plasmid-driven expression of betaKlotho in Ishikawa cells abolished 17beta-estradiol-induced EMT via inhibiting ERK1/2 signaling. Estradiol 155-171 mitogen-activated protein kinase 3 Homo sapiens 199-205 26824324-9 2016 In conclusion, metformin abolishes 17beta-estradiol-induced cell proliferation and EMT in endometrial adenocarcinoma cells by upregulating betaKlotho expression, inhibiting ERK1/2 signaling, and activating AMPKalpha signaling. Estradiol 35-51 mitogen-activated protein kinase 3 Homo sapiens 173-179 26253279-5 2015 17beta-estradiol, propylparaben, and butylparaben increased phosphorylation of ERK1/2 in MCF-7 cells, whereas 17beta-estradiol, methyl- and butylparaben, but not propylparaben, increased phosphorylation of ERK1/2 in MCF-10A cells. Estradiol 0-16 mitogen-activated protein kinase 3 Homo sapiens 79-85 26253279-5 2015 17beta-estradiol, propylparaben, and butylparaben increased phosphorylation of ERK1/2 in MCF-7 cells, whereas 17beta-estradiol, methyl- and butylparaben, but not propylparaben, increased phosphorylation of ERK1/2 in MCF-10A cells. Estradiol 0-16 mitogen-activated protein kinase 3 Homo sapiens 206-212 25608994-0 2014 [Estradiol activates MAPK signaling pathway by estrogen induced VEGF and bFGF in endometrial cancer cells]. Estradiol 1-10 mitogen-activated protein kinase 3 Homo sapiens 21-25 25739982-8 2015 These data indicated that 17beta-estradiol enhances Sertoli cell proliferation via mTOR activation, which involves both ERK1/2 and PI3K/AKT signaling. Estradiol 26-42 mitogen-activated protein kinase 3 Homo sapiens 120-126 25413376-6 2015 In addition, we show that 17beta-oestradiol induces the ERK1/2 activation and increases c-Fos expression through GPR30 associated with ERbeta down-regulation in TCam-2 cell line. Estradiol 26-43 mitogen-activated protein kinase 3 Homo sapiens 56-62 25319474-3 2015 In this study, we evaluate the effect of two fertilizing drugs, clomiphene citrate and estradiol, on the activation of Erk1/2 and the viability of two breast cancer cell lines, MCF-7 (hormone dependent) and BT20 (hormone independent).We show that both drugs induce Erk1/2 phosphorylation in MCF-7 and BT20 cells despite their opposite effect on cell viability. Estradiol 87-96 mitogen-activated protein kinase 3 Homo sapiens 119-125 25319474-3 2015 In this study, we evaluate the effect of two fertilizing drugs, clomiphene citrate and estradiol, on the activation of Erk1/2 and the viability of two breast cancer cell lines, MCF-7 (hormone dependent) and BT20 (hormone independent).We show that both drugs induce Erk1/2 phosphorylation in MCF-7 and BT20 cells despite their opposite effect on cell viability. Estradiol 87-96 mitogen-activated protein kinase 3 Homo sapiens 265-271 25608994-1 2014 OBJECTIVE: To explore the effects of mitogen-activated protein kinase (MAPK) pathway by estradiol induced vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) in endometrial cancer Ishikawa cells. Estradiol 88-97 mitogen-activated protein kinase 3 Homo sapiens 71-75 25608994-3 2014 (1) Western blot analysis was used to detect phosphorylation extracellular signal-regulated kinase 1/2(p-ERK1/2) protein expression with stimulation in different concentrations of estradiol (0.01,0.1, 1, 10, 100 micromol/L). Estradiol 180-189 mitogen-activated protein kinase 3 Homo sapiens 105-111 25608994-6 2014 RESULTS: The expression of the p-ERK1/2 protein at 0.01,0.1, 1, 10, 100 micromol/L were 0.16+-0.03, 0.10+-0.03, 0.41+-0.04, 0.19+-0.03, 0.19+-0.03, there were significantly higher than that in control group (0.05+-0.00, P < 0.05), and which was more obvious at the concentration of 1 micromol/L estradiol. Estradiol 298-307 mitogen-activated protein kinase 3 Homo sapiens 33-39 25608994-15 2014 CONCLUSION: Estradiol inducing the production of VEGF and bFGF could activate MAPK pathway through ER-independent manner, further promote development. Estradiol 12-21 mitogen-activated protein kinase 3 Homo sapiens 78-82 24481325-6 2014 Tamoxifen (TAM) in addition to 17beta-estradiol (E2) and the GPER agonist G1 activated GPER, resulting in transient increases in cell index, intracellular calcium, and ERK1/2 phosphorylation. Estradiol 49-51 mitogen-activated protein kinase 3 Homo sapiens 168-174 24874276-6 2014 Treatment with 17beta-estradiol (E2), the GPER-specific agonist G-1 and tamoxifen (TAM) led to rapid activation of p-ERK1/2, but not p-Akt. Estradiol 15-31 mitogen-activated protein kinase 3 Homo sapiens 117-123 24811094-0 2014 SPRY4-mediated ERK1/2 signaling inhibition abolishes 17beta-estradiol-induced cell growth in endometrial adenocarcinoma cell. Estradiol 53-69 mitogen-activated protein kinase 3 Homo sapiens 15-21 23419500-0 2013 Dual-specificity phosphatase 6 (Dusp6), a negative regulator of FGF2/ERK1/2 signaling, enhances 17beta-estradiol-induced cell growth in endometrial adenocarcinoma cell. Estradiol 96-112 mitogen-activated protein kinase 3 Homo sapiens 69-75