PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 22929836-7 2012 Nitric oxide (NO) synthase which comes in three isoforms, as inducible-, neuronal- and endothelial-NOS, or iNOS, nNOS or eNOS, respectively, catalyzes the conversion of L- arginine to L-citrulline, using NADPH to produce NO. Citrulline 184-196 nitric oxide synthase 3 Homo sapiens 121-125 22402736-6 2012 Endothelial nitric oxide synthase (eNOS) activation was measured by conversion of L-arginine to L-citrulline in endothelial cells incubated with HDL from 49 subjects. Citrulline 96-108 nitric oxide synthase 3 Homo sapiens 0-33 23109853-1 2012 l-Arginine (Arg) is oxidized to l-citrulline and nitric oxide (NO) by the action of endothelial nitric oxide synthase (NOS). Citrulline 32-44 nitric oxide synthase 3 Homo sapiens 84-117 22542797-3 2012 Enzymatic activity of eNOS and intracellular BH4 levels were assessed by means of an arginine-citrulline conversion assay and HPLC analysis, respectively. Citrulline 94-104 nitric oxide synthase 3 Homo sapiens 22-26 22768143-3 2012 Citrulline formation and cytochrome c reduction assays revealed that in comparison with nNOS or iNOS, eNOS was less stringent in the requirement of calcium binding to each of four calcium-binding sites. Citrulline 0-10 nitric oxide synthase 3 Homo sapiens 102-106 21172428-1 2011 The homodimeric flavohemeprotein endothelial nitric oxide synthase (eNOS) oxidizes l-arginine to l-citrulline and nitric oxide (NO), which acutely vasodilates blood vessels and inhibits platelet aggregation. Citrulline 97-109 nitric oxide synthase 3 Homo sapiens 33-66 21839088-3 2011 Our previous data with human umbilical vein (HUVEC) and EA.hy.926 endothelial cells demonstrated that eNOS can obtain its substrate from the conversion of l-citrulline to l-arginine and from protein breakdown. Citrulline 155-167 nitric oxide synthase 3 Homo sapiens 102-106 21839088-4 2011 In the present study, we determined the quantitative contribution of proteasomal and lysosomal protein degradation and investigated to what extent extracellular peptides and l-citrulline can provide substrate to eNOS. Citrulline 174-186 nitric oxide synthase 3 Homo sapiens 212-216 21839088-8 2011 eNOS activity was fully restored by supplementing either l-citrulline or l-arginine-containing dipeptides. Citrulline 57-69 nitric oxide synthase 3 Homo sapiens 0-4 21839088-11 2011 Our study thus demonstrates that l-citrulline and l-arginine-containing peptides derived from either intracellular protein breakdown or from the extracellular space seem to be good substrate sources for eNOS. Citrulline 33-45 nitric oxide synthase 3 Homo sapiens 203-207 21172428-1 2011 The homodimeric flavohemeprotein endothelial nitric oxide synthase (eNOS) oxidizes l-arginine to l-citrulline and nitric oxide (NO), which acutely vasodilates blood vessels and inhibits platelet aggregation. Citrulline 97-109 nitric oxide synthase 3 Homo sapiens 68-72 20593161-5 2010 Endothelial nitric oxide synthase (eNOS) activation was assessed by evaluating the phosphorylation status of the enzyme and evaluating eNOS activity by citrulline synthesis. Citrulline 152-162 nitric oxide synthase 3 Homo sapiens 0-33 19526276-2 2009 NO is synthesized through the conversion of L-arginine to L-citrulline by the enzyme NO synthase (NOS), which is found in three isoforms classified as neuronal (nNOS), inducible (iNOS), and endothelial (eNOS). Citrulline 58-70 nitric oxide synthase 3 Homo sapiens 203-207 20144727-0 2010 Activation of endothelial nitric oxide synthase by the pro-apoptotic drug embelin: Striking discrepancy between nitric oxide-mediated cyclic GMP accumulation and L-citrulline formation. Citrulline 162-174 nitric oxide synthase 3 Homo sapiens 14-47 19176602-4 2009 Activity of NOS3 was characterized by conversion of arginine to citrulline, BH(4) intracellular availability, cGMP, and superoxide anion production. Citrulline 64-74 nitric oxide synthase 3 Homo sapiens 12-16 19617312-2 2009 NO is produced by endothelial NO synthase (eNOS) in the pulmonary vascular endothelium using l-arginine as a substrate and producing l-citrulline as a byproduct. Citrulline 133-145 nitric oxide synthase 3 Homo sapiens 43-47 19617312-3 2009 l-Citrulline is metabolized to l-arginine by two enzymes that are colocated with eNOS in pulmonary vascular endothelial cells. Citrulline 0-12 nitric oxide synthase 3 Homo sapiens 81-85 19513607-4 2009 The results showed that incubation of 293-eNOS cells with simvastatin (10 microm/L) for 2 h significantly increased in the activity of eNOS as shown by the conversion of L-arginine to L-citrulline (2889.70+/-201.51 versus 5630.18+/-218.75 pmol/min . Citrulline 184-196 nitric oxide synthase 3 Homo sapiens 135-139 18375203-1 2008 Vascular endothelial nitric oxide (NO) production via the citrulline-NO cycle not only involves the regulation of endothelial nitric oxide synthase (eNOS), but also regulation of caveolar-localized endothelial argininosuccinate synthase (AS), which catalyzes the rate-limiting step of the cycle. Citrulline 58-68 nitric oxide synthase 3 Homo sapiens 114-147 18564176-5 2009 A significant increase in endothelial NO synthase (eNOS) activity, measured in terms of citrulline production, was observed with glimepiride treatment. Citrulline 88-98 nitric oxide synthase 3 Homo sapiens 51-55 17229561-3 2007 In control medium, eNOS activity (evaluated by the citrulline assay) and eNOS phosphorylation on Ser 1177 were correlated. Citrulline 51-61 nitric oxide synthase 3 Homo sapiens 19-23 17869551-1 2007 Nitric oxide (NO) is an important vasorelaxant produced along with L-citrulline from L-arginine in a reaction catalyzed by endothelial nitric oxide synthase (eNOS). Citrulline 67-79 nitric oxide synthase 3 Homo sapiens 123-156 17427197-6 2007 TGF-beta1 and high D-glucose increased hCAT-1 mRNA expression ( approximately 8-fold) and maximal transport velocity (V(max)), L-[(3)H]citrulline formation from L-[(3)H]arginine (index of NO synthesis) and endothelial NO synthase (eNOS) protein abundance, but did not alter eNOS phosphorylation. Citrulline 135-145 nitric oxide synthase 3 Homo sapiens 231-235 17427197-6 2007 TGF-beta1 and high D-glucose increased hCAT-1 mRNA expression ( approximately 8-fold) and maximal transport velocity (V(max)), L-[(3)H]citrulline formation from L-[(3)H]arginine (index of NO synthesis) and endothelial NO synthase (eNOS) protein abundance, but did not alter eNOS phosphorylation. Citrulline 135-145 nitric oxide synthase 3 Homo sapiens 274-278 17116549-6 2006 Endothelial nitric oxide synthase (eNOS) activity was assayed by measuring conversion of L-arginine to L-citrulline. Citrulline 103-115 nitric oxide synthase 3 Homo sapiens 0-33 16585403-2 2006 A functional eNOS oxidizes its substrate L-arginine to L-citrulline and NO*. Citrulline 55-67 nitric oxide synthase 3 Homo sapiens 13-17 16497987-4 2006 Ox-PAPC treatment of EC induced a dose- and time-dependent activation of eNOS, as measured by phosphorylation of serine 1177, dephosphorylation of threonine 495, and the conversion of L-arginine to L-citrulline. Citrulline 198-210 nitric oxide synthase 3 Homo sapiens 73-77 15655515-7 2005 Active eNOS was detected by quantifying conversion of L-arginine to L-citrulline and by measuring NO released from endothelial cells using the fluorescent probe DAF-2 (20-96 h).eNOS promoter activity increased in response to isoflavone treatment (20 h). Citrulline 68-80 nitric oxide synthase 3 Homo sapiens 7-11 15819614-4 2005 The loss of immunoprecipitable eNOS from Cav-1-enriched fractions was accompanied by a decrease both in phosphorylation of eNOS and in enzymatic activity (conversion of arginine into citrulline). Citrulline 183-193 nitric oxide synthase 3 Homo sapiens 31-35 15655515-7 2005 Active eNOS was detected by quantifying conversion of L-arginine to L-citrulline and by measuring NO released from endothelial cells using the fluorescent probe DAF-2 (20-96 h).eNOS promoter activity increased in response to isoflavone treatment (20 h). Citrulline 68-80 nitric oxide synthase 3 Homo sapiens 177-181 15465788-1 2004 L-arginine, the principal substrate for endothelial nitric oxide synthase, is oxidized to L-citrulline and nitric oxide. Citrulline 90-102 nitric oxide synthase 3 Homo sapiens 40-73 14599552-5 2003 However, if sufficient citrulline is present in the medium, A23187-activated NO production by eNOS does not rely on extracellular arginine. Citrulline 23-33 nitric oxide synthase 3 Homo sapiens 94-98 15288120-4 2004 Ionomycin induced citrulline and cGMP formation partially through phosphorylation of endothelial NO synthase (eNOS) at its serine residue 1177, which was mediated mainly by calmodulin-dependent kinase II. Citrulline 18-28 nitric oxide synthase 3 Homo sapiens 110-114 11243424-4 2001 L-Citrulline formation (a measure of eNOS enzymatic activity) was significantly increased in cells treated for 24 h with vitamin C. Citrulline 0-12 nitric oxide synthase 3 Homo sapiens 37-41 12756290-1 2003 The enzyme endothelial nitric oxide synthase (eNOS) catalyzes the conversion of arginine, oxygen and NADPH to NO and citrulline. Citrulline 117-127 nitric oxide synthase 3 Homo sapiens 11-44 12756290-1 2003 The enzyme endothelial nitric oxide synthase (eNOS) catalyzes the conversion of arginine, oxygen and NADPH to NO and citrulline. Citrulline 117-127 nitric oxide synthase 3 Homo sapiens 46-50 11443071-6 2001 Diamide treatment of either PAECs, PAEC membrane fractions, or purified endothelial nitric oxide synthase (eNOS) resulted in significant inhibition (approximately 75%) of eNOS catalytic activity measured as L-[(3)H]arginine-to-L-[(3)H]citrulline conversion. Citrulline 235-245 nitric oxide synthase 3 Homo sapiens 72-105 11443071-6 2001 Diamide treatment of either PAECs, PAEC membrane fractions, or purified endothelial nitric oxide synthase (eNOS) resulted in significant inhibition (approximately 75%) of eNOS catalytic activity measured as L-[(3)H]arginine-to-L-[(3)H]citrulline conversion. Citrulline 235-245 nitric oxide synthase 3 Homo sapiens 107-111 8764145-4 1996 We observed that cNOS activity could be quantitated in islet homogenates by monitoring the formation of L-citrulline from L-arginine using an Amprep CBA cation-exhange minicolumn before derivatization with o-phthaldialdehyde and subsequent high-performance liquid chromatography analysis. Citrulline 104-116 nitric oxide synthase 3 Homo sapiens 17-21 9242548-6 1997 The eNOS transfectants were shown to contain functional enzyme by the conversion of L-arginine to L-citrulline in fractionated cells (P = .0001) and by exposing intact cells to calcium ionophore using the cGMP reporter cell assay (P = .0001). Citrulline 98-110 nitric oxide synthase 3 Homo sapiens 4-8 9077471-6 1997 The Ca2+-dependent NOS (cNOS) activity in the cytosol of HSc fibroblasts (1.43 pmol/min/g of protein) was significantly lower than that in normal fibroblasts (2.60 pmol/min/g of protein), as determined by citrulline assay (p < 0.01, n = 4). Citrulline 205-215 nitric oxide synthase 3 Homo sapiens 4-22 9077471-6 1997 The Ca2+-dependent NOS (cNOS) activity in the cytosol of HSc fibroblasts (1.43 pmol/min/g of protein) was significantly lower than that in normal fibroblasts (2.60 pmol/min/g of protein), as determined by citrulline assay (p < 0.01, n = 4). Citrulline 205-215 nitric oxide synthase 3 Homo sapiens 24-28 9065411-4 1997 In fact, a 30-min treatment of T67 cells with the combination of lipopolysaccharide plus interferon-gamma (MIX) strongly inhibits the cNOS activity, as determined by measuring [3H]citrulline production. Citrulline 180-190 nitric oxide synthase 3 Homo sapiens 134-138 7514511-7 1994 The L-citrulline assay revealed a twofold increase in cNOS activity on increasing pH from 6.7 to 7.4, an optimum at pH 7.5, and a complete abolition of activity at pH 8.6. Citrulline 4-16 nitric oxide synthase 3 Homo sapiens 54-58 7488171-1 1995 Endothelial nitric oxide synthase (eNOS) is an important oxygenase which catalyzes the conversion of L-arginine to L-citrulline to form nitric oxide (NO), a potent important factor for vasodilation and inhibition of platelet aggregation. Citrulline 115-127 nitric oxide synthase 3 Homo sapiens 0-33 7488171-1 1995 Endothelial nitric oxide synthase (eNOS) is an important oxygenase which catalyzes the conversion of L-arginine to L-citrulline to form nitric oxide (NO), a potent important factor for vasodilation and inhibition of platelet aggregation. Citrulline 115-127 nitric oxide synthase 3 Homo sapiens 35-39 32814314-3 2020 It is produced in endothelial cells by endothelial NO synthase (eNOS) that mediates the conversion of the amino acid arginine into NO and citrulline. Citrulline 138-148 nitric oxide synthase 3 Homo sapiens 39-62 33499404-1 2021 Nitric oxide (NO) is formed during the oxidation of L-arginine to L-citrulline by the action of multiple isoenzymes of NO synthase (NOS): neuronal NOS (nNOS), endotelial NOS (eNOS), and inducible NOS (iNOS). Citrulline 66-78 nitric oxide synthase 3 Homo sapiens 175-179 32814314-3 2020 It is produced in endothelial cells by endothelial NO synthase (eNOS) that mediates the conversion of the amino acid arginine into NO and citrulline. Citrulline 138-148 nitric oxide synthase 3 Homo sapiens 64-68 29969842-12 2018 The eNOS activity was 52.0 +- 20.6 pmol l-citrulline/min/106 ECs in nonsmokers and 48.7 +- 19.8 pmol l-citrulline/min/106 ECs in environmental smokers. Citrulline 40-52 nitric oxide synthase 3 Homo sapiens 4-8 29969842-12 2018 The eNOS activity was 52.0 +- 20.6 pmol l-citrulline/min/106 ECs in nonsmokers and 48.7 +- 19.8 pmol l-citrulline/min/106 ECs in environmental smokers. Citrulline 101-113 nitric oxide synthase 3 Homo sapiens 4-8 26296888-1 2015 Citrulline formation by both human neuronal nitric-oxide synthase (nNOS) and mouse macrophage inducible NOS was inhibited by the hydrogen sulfide (H2S) donor Na2S with IC50 values of ~2.4 10(-5) and ~7.9 10(-5) m, respectively, whereas human endothelial NOS was hardly affected at all. Citrulline 0-10 nitric oxide synthase 3 Homo sapiens 242-257 28877324-8 2017 Normalization of nitric oxide levels with l-arginine 9 g/d or l-citrulline 800 mg/d, both of which relieve vasospastic angina in Prinzmetal"s angina, which has the same eNOS genotype as primary osteonecrosis, may slow or stop the progression of osteonecrosis. Citrulline 62-74 nitric oxide synthase 3 Homo sapiens 169-173 27417628-5 2016 Supplemental citrulline could be a practical option for primary or secondary prevention of cardiovascular events and mortality, as it is inexpensive, has a mild flavor, and is well tolerated in doses (3-6 g daily) that can influence eNOS activity. Citrulline 13-23 nitric oxide synthase 3 Homo sapiens 233-237 25536367-11 2015 When started after disease onset, oral L-citrulline treatment improves NO production by recoupling eNOS and inhibits the further development of chronic hypoxia-induced pulmonary hypertension in newborn piglets. Citrulline 39-51 nitric oxide synthase 3 Homo sapiens 99-103 26286023-1 2015 Endothelial nitric oxide synthase (eNOS) catalyzes the conversion of l-arginine and molecular oxygen into l-citrulline and nitric oxide (NO), a gaseous second messenger that influences cardiovascular physiology and disease. Citrulline 106-118 nitric oxide synthase 3 Homo sapiens 0-33 26286023-1 2015 Endothelial nitric oxide synthase (eNOS) catalyzes the conversion of l-arginine and molecular oxygen into l-citrulline and nitric oxide (NO), a gaseous second messenger that influences cardiovascular physiology and disease. Citrulline 106-118 nitric oxide synthase 3 Homo sapiens 35-39 25536367-5 2015 The objective of this study was to determine whether starting L-citrulline after the onset of pulmonary hypertension inhibits disease progression and improves NO production by recoupling endothelial NO synthase (eNOS). Citrulline 62-74 nitric oxide synthase 3 Homo sapiens 212-216 25536367-10 2015 NO production and eNOS dimer-to-monomer ratios were greater in pulmonary arteries from L-citrulline-treated than from untreated hypoxic animals but were lower than in normoxic controls. Citrulline 87-99 nitric oxide synthase 3 Homo sapiens 18-22 23847624-3 2013 In endothelial cells, NO is generated by endothelial nitric oxide synthase (eNOS) through the conversion of its substrate, l-arginine to l-citrulline. Citrulline 137-149 nitric oxide synthase 3 Homo sapiens 41-74