PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 10683762-3 1999 The concentrations of cyclin D1 and p21Waf1/Cip1 were dramatically increased, whereas those of cyclin B1 and cdc2 were decreased, by PMA treatment. Tetradecanoylphorbol Acetate 133-136 cyclin dependent kinase 1 Homo sapiens 109-113 15302583-8 2004 In summary, TPA-induced TIS21 mRNA expression is mediated by PKC-delta, and TIS21 induces G2/M arrest and cell death by inhibiting cyclin B1-Cdc2 binding and the kinase activity through its binding to Cdc2. Tetradecanoylphorbol Acetate 12-15 cyclin dependent kinase 1 Homo sapiens 141-145 15302583-8 2004 In summary, TPA-induced TIS21 mRNA expression is mediated by PKC-delta, and TIS21 induces G2/M arrest and cell death by inhibiting cyclin B1-Cdc2 binding and the kinase activity through its binding to Cdc2. Tetradecanoylphorbol Acetate 12-15 cyclin dependent kinase 1 Homo sapiens 201-205 12963846-6 2003 IkappaBalphaM cells also displayed impairment in NaB- and PMA-mediated induction of p21CIP1 and phosphorylation (inactivation) of p34cdc2, as well as diminished levels of pRb-bound E2F1. Tetradecanoylphorbol Acetate 58-61 cyclin dependent kinase 1 Homo sapiens 130-137 12061815-8 2002 SB/PMA treatment also triggered a decline in the S and G(2)M populations, and dephosphorylation of p34(cdc2). Tetradecanoylphorbol Acetate 3-6 cyclin dependent kinase 1 Homo sapiens 103-107 9438385-0 1998 Taxol-induced p34cdc2 kinase activation and apoptosis inhibited by 12-O-tetradecanoylphorbol-13-acetate in human breast MCF-7 carcinoma cells. Tetradecanoylphorbol Acetate 67-103 cyclin dependent kinase 1 Homo sapiens 14-21 9683525-0 1998 Regulation of the cell cycle at the G2/M boundary in metastatic melanoma cells by 12-O-tetradecanoyl phorbol-13-acetate (TPA) by blocking p34cdc2 kinase activity. Tetradecanoylphorbol Acetate 82-119 cyclin dependent kinase 1 Homo sapiens 138-145 9683525-0 1998 Regulation of the cell cycle at the G2/M boundary in metastatic melanoma cells by 12-O-tetradecanoyl phorbol-13-acetate (TPA) by blocking p34cdc2 kinase activity. Tetradecanoylphorbol Acetate 121-124 cyclin dependent kinase 1 Homo sapiens 138-145 9683525-7 1998 Examination of the levels of cyclins A and B1 demonstrated that the levels of these cyclins were not limiting for entrance into M. However, the addition of TPA blocked the increase in p34(cdc2)/cyclin B1 kinase activity. Tetradecanoylphorbol Acetate 156-159 cyclin dependent kinase 1 Homo sapiens 188-192 9683525-8 1998 In cells treated with TPA, most p34(cdc2) was found in the slowly migrating forms on Western blots, which contained increased levels of phosphotyrosine. Tetradecanoylphorbol Acetate 22-25 cyclin dependent kinase 1 Homo sapiens 36-40 9168444-8 1997 TPA delayed DNA synthesis and expression of these genes, and suppressed Cdc2 kinase activity in the second round G2/M phase. Tetradecanoylphorbol Acetate 0-3 cyclin dependent kinase 1 Homo sapiens 72-76 9438385-8 1998 TPA inhibited Taxol-mediated activation of p34cdc2 kinase by preventing the dephosphorylation of the Tyr-15 residue on p34cdc2 without altering the levels of Cdc2 and cyclin B1. Tetradecanoylphorbol Acetate 0-3 cyclin dependent kinase 1 Homo sapiens 43-50 9438385-8 1998 TPA inhibited Taxol-mediated activation of p34cdc2 kinase by preventing the dephosphorylation of the Tyr-15 residue on p34cdc2 without altering the levels of Cdc2 and cyclin B1. Tetradecanoylphorbol Acetate 0-3 cyclin dependent kinase 1 Homo sapiens 119-126 8816913-1 1996 Studies on the link between cellular signalling and cell cycle control at the G2 checkpoint have shown that, in HeLa cells, epidermal growth factor (EGF) and the phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA) rapidly inhibit the G2-M transition by preventing the key component of mitosis-promoting factor (MPF), p34cdc2, from expressing protein kinase activity. Tetradecanoylphorbol Acetate 176-212 cyclin dependent kinase 1 Homo sapiens 322-329 8761290-8 1996 In addition, TPA-treated cells showed high levels of cyclin B and cdc2 proteins, however no activation of cdc2 was detected. Tetradecanoylphorbol Acetate 13-16 cyclin dependent kinase 1 Homo sapiens 66-70 7589260-3 1995 To investigate the mechanism by which TPA arrests melanoma cell growth at the G1/S transition we have examined its effects on the levels of cyclins and cyclin dependent kinases (CDKs) and activation of CDK2 kinase activity. Tetradecanoylphorbol Acetate 38-41 cyclin dependent kinase 1 Homo sapiens 178-182 7589260-4 1995 Addition of TPA in G1 blocked the increase in the level of p34cdc2 mRNA, but not of CDK2 mRNA. Tetradecanoylphorbol Acetate 12-15 cyclin dependent kinase 1 Homo sapiens 59-66 7760824-4 1995 Intriguingly, TPA-mediated repression of the cdc2 promoter was independent of the transcription factor E2F, distinguishing this pathway from mechanisms responsible for repression of cdc2 transcription in response to serum starvation. Tetradecanoylphorbol Acetate 14-17 cyclin dependent kinase 1 Homo sapiens 45-49 7760824-4 1995 Intriguingly, TPA-mediated repression of the cdc2 promoter was independent of the transcription factor E2F, distinguishing this pathway from mechanisms responsible for repression of cdc2 transcription in response to serum starvation. Tetradecanoylphorbol Acetate 14-17 cyclin dependent kinase 1 Homo sapiens 182-186 7760824-7 1995 Analysis of THP1 nuclear proteins revealed a 55-kDa protein that was induced by TPA and interacted with the cdc2 promoter in an R-box-dependent manner. Tetradecanoylphorbol Acetate 80-83 cyclin dependent kinase 1 Homo sapiens 108-112 1390335-0 1992 12-O-tetradecanoylphorbol-13-acetate induces transient cell cycle arrest in G1 and G2 in metastatic melanoma cells: inhibition of phosphorylation of p34cdc2. Tetradecanoylphorbol Acetate 0-36 cyclin dependent kinase 1 Homo sapiens 149-156 1390335-4 1992 In addition, TPA induced a pronounced morphological change, which peaked by 1 h and gradually subsided over 24 h. In populations of cells synchronized in G1 using lovastatin, (a) addition of TPA blocked the onset of DNA synthesis up to the end of G1; (b) the lag between addition of the drug and onset of DNA synthesis was less than 30 min; and (c) addition of TPA at the end of G1 prevented the increased phosphorylation of p34cdc2, as determined by immunoprecipitation. Tetradecanoylphorbol Acetate 13-16 cyclin dependent kinase 1 Homo sapiens 425-432 1390335-4 1992 In addition, TPA induced a pronounced morphological change, which peaked by 1 h and gradually subsided over 24 h. In populations of cells synchronized in G1 using lovastatin, (a) addition of TPA blocked the onset of DNA synthesis up to the end of G1; (b) the lag between addition of the drug and onset of DNA synthesis was less than 30 min; and (c) addition of TPA at the end of G1 prevented the increased phosphorylation of p34cdc2, as determined by immunoprecipitation. Tetradecanoylphorbol Acetate 191-194 cyclin dependent kinase 1 Homo sapiens 425-432 1390335-4 1992 In addition, TPA induced a pronounced morphological change, which peaked by 1 h and gradually subsided over 24 h. In populations of cells synchronized in G1 using lovastatin, (a) addition of TPA blocked the onset of DNA synthesis up to the end of G1; (b) the lag between addition of the drug and onset of DNA synthesis was less than 30 min; and (c) addition of TPA at the end of G1 prevented the increased phosphorylation of p34cdc2, as determined by immunoprecipitation. Tetradecanoylphorbol Acetate 191-194 cyclin dependent kinase 1 Homo sapiens 425-432 8187833-0 1994 Phorbol ester TPA rapidly prevents activation of p34cdc2 histone H1 kinase and concomitantly the transition from G2 phase to mitosis in synchronized HeLa cells. Tetradecanoylphorbol Acetate 14-17 cyclin dependent kinase 1 Homo sapiens 49-56 8187833-4 1994 Treatment of HeLa cells (synchronized around the G2-M transition) with TPA (10(-7) M) has now been shown to induce an overall decrease of the histone H1 kinase activity associated with anti-p34cdc2 immunoprecipitates after about 20 to 30 min. Tetradecanoylphorbol Acetate 71-74 cyclin dependent kinase 1 Homo sapiens 190-197 8187833-8 1994 However, p34cdc2 from cultures treated with TPA was more intensely stained by anti-phosphotyrosine antibodies than that of control cells, indicating that TPA treatment probably prevented the tyrosine dephosphorylation required for expression of the histone H1 kinase activity of the complex. Tetradecanoylphorbol Acetate 44-47 cyclin dependent kinase 1 Homo sapiens 9-16 8187833-8 1994 However, p34cdc2 from cultures treated with TPA was more intensely stained by anti-phosphotyrosine antibodies than that of control cells, indicating that TPA treatment probably prevented the tyrosine dephosphorylation required for expression of the histone H1 kinase activity of the complex. Tetradecanoylphorbol Acetate 154-157 cyclin dependent kinase 1 Homo sapiens 9-16 8187833-9 1994 The results indicate that TPA treatment of HeLa cultures rapidly stops the G2-M transition because it very rapidly prevents the p34cdc2/Cyclin B complex in G2 cells from developing histone H1 kinase activity. Tetradecanoylphorbol Acetate 26-29 cyclin dependent kinase 1 Homo sapiens 128-135 25019390-10 2014 Treatment with the PKC agonist phorbol 12-myristate 13-acetate (PMA) increased phosphorylation of PKC substrates and MAPK and increased p34cdc2 kinase activity. Tetradecanoylphorbol Acetate 31-62 cyclin dependent kinase 1 Homo sapiens 136-143 25019390-10 2014 Treatment with the PKC agonist phorbol 12-myristate 13-acetate (PMA) increased phosphorylation of PKC substrates and MAPK and increased p34cdc2 kinase activity. Tetradecanoylphorbol Acetate 64-67 cyclin dependent kinase 1 Homo sapiens 136-143 20554189-0 2010 12-O-Tetradecanoyl phorbol-13-acetate (TPA)-induced growth arrest is increased by silibinin by the down-regulation of cyclin B1 and cdc2 and the up-regulation of p21 expression in MDA-MB231 human breast cancer cells. Tetradecanoylphorbol Acetate 0-37 cyclin dependent kinase 1 Homo sapiens 132-136 20554189-0 2010 12-O-Tetradecanoyl phorbol-13-acetate (TPA)-induced growth arrest is increased by silibinin by the down-regulation of cyclin B1 and cdc2 and the up-regulation of p21 expression in MDA-MB231 human breast cancer cells. Tetradecanoylphorbol Acetate 39-42 cyclin dependent kinase 1 Homo sapiens 132-136 20554189-6 2010 Under the same conditions, TPA-induced down-regulation of cyclin B1 and cdc2 was decreased by silibinin. Tetradecanoylphorbol Acetate 27-30 cyclin dependent kinase 1 Homo sapiens 72-76