PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 9486215-2 1998 The proinflammatory cytokine tumor necrosis factor-alpha (TNF-alpha) and the phorbol ester 12-O-tetradecanoyl phorbol-13-acetate (TPA) inhibit expression of surfactant-associated proteins A and B (SP-A and SP-B), both important for normal surfactant function. Tetradecanoylphorbol Acetate 91-128 surfactant protein B Homo sapiens 206-210 9486215-2 1998 The proinflammatory cytokine tumor necrosis factor-alpha (TNF-alpha) and the phorbol ester 12-O-tetradecanoyl phorbol-13-acetate (TPA) inhibit expression of surfactant-associated proteins A and B (SP-A and SP-B), both important for normal surfactant function. Tetradecanoylphorbol Acetate 130-133 surfactant protein B Homo sapiens 206-210 9294011-1 1997 Phorbol esters such as 12-O-tetradecanoylphorbol-13-acetate (TPA) activate protein kinase C and have been previously shown to down-regulate surfactant proteins SP-A and SP-B in H441 adenocarcinoma cells. Tetradecanoylphorbol Acetate 23-59 surfactant protein B Homo sapiens 169-173 9252399-3 1997 Responsiveness to 12-O-tetradecanoylphorbol-13-acetate (TPA) localized to the SP-B proximal promoter (-140/-65 bp) and specifically to binding sites for TTF-1 and HNF3, which act as cell-specific enhancers of SP-B expression. Tetradecanoylphorbol Acetate 18-54 surfactant protein B Homo sapiens 78-82 9252399-3 1997 Responsiveness to 12-O-tetradecanoylphorbol-13-acetate (TPA) localized to the SP-B proximal promoter (-140/-65 bp) and specifically to binding sites for TTF-1 and HNF3, which act as cell-specific enhancers of SP-B expression. Tetradecanoylphorbol Acetate 18-54 surfactant protein B Homo sapiens 209-213 9252399-3 1997 Responsiveness to 12-O-tetradecanoylphorbol-13-acetate (TPA) localized to the SP-B proximal promoter (-140/-65 bp) and specifically to binding sites for TTF-1 and HNF3, which act as cell-specific enhancers of SP-B expression. Tetradecanoylphorbol Acetate 56-59 surfactant protein B Homo sapiens 78-82 9252399-3 1997 Responsiveness to 12-O-tetradecanoylphorbol-13-acetate (TPA) localized to the SP-B proximal promoter (-140/-65 bp) and specifically to binding sites for TTF-1 and HNF3, which act as cell-specific enhancers of SP-B expression. Tetradecanoylphorbol Acetate 56-59 surfactant protein B Homo sapiens 209-213 9294011-1 1997 Phorbol esters such as 12-O-tetradecanoylphorbol-13-acetate (TPA) activate protein kinase C and have been previously shown to down-regulate surfactant proteins SP-A and SP-B in H441 adenocarcinoma cells. Tetradecanoylphorbol Acetate 61-64 surfactant protein B Homo sapiens 169-173 9294011-4 1997 In cells cultured in the presence of dexamethasone, which increases the low basal level of SP-B expression, TPA decreased both SP-B mRNA content (approximately 8% of control) and rate of transcription (7% of control). Tetradecanoylphorbol Acetate 108-111 surfactant protein B Homo sapiens 91-95 9294011-4 1997 In cells cultured in the presence of dexamethasone, which increases the low basal level of SP-B expression, TPA decreased both SP-B mRNA content (approximately 8% of control) and rate of transcription (7% of control). Tetradecanoylphorbol Acetate 108-111 surfactant protein B Homo sapiens 127-131 9294011-5 1997 In cultured human fetal lung explants, TPA decreased SP-A and SP-B protein and mRNA in a time- and dose-dependent fashion, with half-maximal effect on mRNAs at approximately 3 nM and approximately 50% inhibition after 24 h of exposure, and similarly reduced SP-A and SP-B gene transcription (approximately 55% of control at 8-24 h). Tetradecanoylphorbol Acetate 39-42 surfactant protein B Homo sapiens 62-66 9294011-5 1997 In cultured human fetal lung explants, TPA decreased SP-A and SP-B protein and mRNA in a time- and dose-dependent fashion, with half-maximal effect on mRNAs at approximately 3 nM and approximately 50% inhibition after 24 h of exposure, and similarly reduced SP-A and SP-B gene transcription (approximately 55% of control at 8-24 h). Tetradecanoylphorbol Acetate 39-42 surfactant protein B Homo sapiens 267-271 9294011-6 1997 We conclude that TPA acts primarily at the level of gene transcription to down-regulate both SP-A and SP-B in H441 and fetal lung cells, and we speculate that inflammatory and other agents that act through PKC may modulate expression of the surfactant proteins and alter surfactant function in vivo. Tetradecanoylphorbol Acetate 17-20 surfactant protein B Homo sapiens 102-106 1616052-4 1992 Inhibitory effects of both agents on SP-A gene transcription were readily detected within 6 h after exposure and persisted for 24 h. While TNF-alpha and TPA decreased cellular SP-B mRNA content, transcription of the SP-B gene was not influenced by these agents. Tetradecanoylphorbol Acetate 153-156 surfactant protein B Homo sapiens 176-180 8048538-0 1994 3"-untranslated region of SP-B mRNA mediates inhibitory effects of TPA and TNF-alpha on SP-B expression. Tetradecanoylphorbol Acetate 67-70 surfactant protein B Homo sapiens 26-30 8048538-0 1994 3"-untranslated region of SP-B mRNA mediates inhibitory effects of TPA and TNF-alpha on SP-B expression. Tetradecanoylphorbol Acetate 67-70 surfactant protein B Homo sapiens 88-92 8048538-4 1994 Inhibitory effects of TPA and TNF-alpha on SP-B mRNA expression in vitro were mediated by decreased SP-B mRNA stability rather than by decreased rate of SP-B gene transcription. Tetradecanoylphorbol Acetate 22-25 surfactant protein B Homo sapiens 43-47 8048538-4 1994 Inhibitory effects of TPA and TNF-alpha on SP-B mRNA expression in vitro were mediated by decreased SP-B mRNA stability rather than by decreased rate of SP-B gene transcription. Tetradecanoylphorbol Acetate 22-25 surfactant protein B Homo sapiens 100-104 8048538-4 1994 Inhibitory effects of TPA and TNF-alpha on SP-B mRNA expression in vitro were mediated by decreased SP-B mRNA stability rather than by decreased rate of SP-B gene transcription. Tetradecanoylphorbol Acetate 22-25 surfactant protein B Homo sapiens 100-104 8048538-6 1994 The mRNAs and cellular growth hormone protein generated from the chimeric TKGH(SP-B2.0) and TKGH(SP-B.837) genes were each inhibited by approximately 50% by TPA and TNF-alpha. Tetradecanoylphorbol Acetate 157-160 surfactant protein B Homo sapiens 79-83 8048538-8 1994 The inhibition of steady-state hGH-SP-B mRNA by TPA and TNF-alpha was mediated by a cis-active element located in the 3-UTR region of SP-B mRNA. Tetradecanoylphorbol Acetate 48-51 surfactant protein B Homo sapiens 35-39 8048538-8 1994 The inhibition of steady-state hGH-SP-B mRNA by TPA and TNF-alpha was mediated by a cis-active element located in the 3-UTR region of SP-B mRNA. Tetradecanoylphorbol Acetate 48-51 surfactant protein B Homo sapiens 134-138 1616052-5 1992 In contrast to the inhibitory effects of TPA and TNF-alpha on SP-A and SP-B mRNAs, steady-state mRNA and rate of transcription of human manganese superoxide dismutase (MnSOD) were increased by both agents. Tetradecanoylphorbol Acetate 41-44 surfactant protein B Homo sapiens 71-75 1616052-10 1992 Actinomycin D blocked the inhibitory effects of TNF-alpha and TPA on SP-A and SP-B mRNA, supporting a role for posttranscriptional events in the modulation of the expression of the surfactant proteins SP-A and SP-B. Tetradecanoylphorbol Acetate 62-65 surfactant protein B Homo sapiens 78-82 1616052-1 1992 Tumor necrosis factor-alpha (TNF-alpha) and the phorbol ester, 12-O-tetradecanoyl-phorbol-13-acetate (TPA) decrease the synthesis of surfactant proteins association with decreased SP-A and SP-B mRNA. Tetradecanoylphorbol Acetate 63-100 surfactant protein B Homo sapiens 189-193 1616052-1 1992 Tumor necrosis factor-alpha (TNF-alpha) and the phorbol ester, 12-O-tetradecanoyl-phorbol-13-acetate (TPA) decrease the synthesis of surfactant proteins association with decreased SP-A and SP-B mRNA. Tetradecanoylphorbol Acetate 102-105 surfactant protein B Homo sapiens 189-193 1616052-10 1992 Actinomycin D blocked the inhibitory effects of TNF-alpha and TPA on SP-A and SP-B mRNA, supporting a role for posttranscriptional events in the modulation of the expression of the surfactant proteins SP-A and SP-B. Tetradecanoylphorbol Acetate 62-65 surfactant protein B Homo sapiens 210-214 2249989-1 1990 Effects of the tumor promoter, 12-O-tetradecanoylphorbol-13-acetate (TPA), on expression of pulmonary surfactant proteins, SP-A and SP-B, were determined in a human pulmonary adenocarcinoma cell line (H441-4). Tetradecanoylphorbol Acetate 69-72 surfactant protein B Homo sapiens 132-136 2249989-7 1990 Expression of a distinct surfactant protein, SP-B, was also markedly decreased after exposure to TPA. Tetradecanoylphorbol Acetate 97-100 surfactant protein B Homo sapiens 45-49 2249989-9 1990 Actinomycin D completely blocked the rapid decrease in SP-A and SP-B mRNAs caused by the phorbol ester, consistent with the concept that the inhibitory effect of TPA on the surfactant protein mRNAs required continued gene transcription and was not mediated solely by changes in SP-A or SP-B transcription. Tetradecanoylphorbol Acetate 162-165 surfactant protein B Homo sapiens 64-68 2249989-8 1990 SP-A and SP-B mRNA contents decreased more rapidly after treatment with TPA than after actinomycin D. Tetradecanoylphorbol Acetate 72-75 surfactant protein B Homo sapiens 9-13 2249989-9 1990 Actinomycin D completely blocked the rapid decrease in SP-A and SP-B mRNAs caused by the phorbol ester, consistent with the concept that the inhibitory effect of TPA on the surfactant protein mRNAs required continued gene transcription and was not mediated solely by changes in SP-A or SP-B transcription. Tetradecanoylphorbol Acetate 162-165 surfactant protein B Homo sapiens 286-290