PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 11986211-3 2002 In the presence of serum and phorbol 12-myristate 13-acetate (PMA), a PKC activator, cells exhibited full megakaryocytic differentiation, manifested by adhesion, shape change, increased cell size, polyploidy, PPF, and expression of CD41(+), CD61(+), and CD62P(+). Tetradecanoylphorbol Acetate 29-60 integrin subunit alpha 2b Homo sapiens 232-236 11986211-3 2002 In the presence of serum and phorbol 12-myristate 13-acetate (PMA), a PKC activator, cells exhibited full megakaryocytic differentiation, manifested by adhesion, shape change, increased cell size, polyploidy, PPF, and expression of CD41(+), CD61(+), and CD62P(+). Tetradecanoylphorbol Acetate 62-65 integrin subunit alpha 2b Homo sapiens 232-236 8632663-3 1995 TPA treatment also enhanced the expression of GPIIb mRNA, and induced the expression of interleukin-6 (IL-6) and its receptor mRNAs, while it did not induce transcripts of the genes IL-11 and mpl ligand, and further decreased the transcript of the mpl gene. Tetradecanoylphorbol Acetate 0-3 integrin subunit alpha 2b Homo sapiens 46-51 8547633-2 1996 Treatment of these cells with 10 nmol/L phorbol 12-myristate 13-acetate (PMA) for 3 days caused a complete inhibition of proliferation and a threefold increase in the surface expression of glycoprotein (GP) IIIa, a marker of megakaryocytic differentiation that forms part of the fibrinogen receptor complex, GPIIb/IIIa. Tetradecanoylphorbol Acetate 40-71 integrin subunit alpha 2b Homo sapiens 308-313 8547633-2 1996 Treatment of these cells with 10 nmol/L phorbol 12-myristate 13-acetate (PMA) for 3 days caused a complete inhibition of proliferation and a threefold increase in the surface expression of glycoprotein (GP) IIIa, a marker of megakaryocytic differentiation that forms part of the fibrinogen receptor complex, GPIIb/IIIa. Tetradecanoylphorbol Acetate 73-76 integrin subunit alpha 2b Homo sapiens 308-313 11146165-10 2000 Expression of CD41, a differentiation-related phenotype, was significantly induced by TPA after 7 days of treatment, showing that functional maturation was mainly induced by TPA. Tetradecanoylphorbol Acetate 86-89 integrin subunit alpha 2b Homo sapiens 14-18 11146165-10 2000 Expression of CD41, a differentiation-related phenotype, was significantly induced by TPA after 7 days of treatment, showing that functional maturation was mainly induced by TPA. Tetradecanoylphorbol Acetate 174-177 integrin subunit alpha 2b Homo sapiens 14-18 11281287-8 2000 The downregulation of alpha-tocopherol on the TPA-mediated GPIIb promoter activity may result in a reduction of GPIIb protein expression and thus contribute to antiplatelet aggregation. Tetradecanoylphorbol Acetate 46-49 integrin subunit alpha 2b Homo sapiens 59-64 11281287-8 2000 The downregulation of alpha-tocopherol on the TPA-mediated GPIIb promoter activity may result in a reduction of GPIIb protein expression and thus contribute to antiplatelet aggregation. Tetradecanoylphorbol Acetate 46-49 integrin subunit alpha 2b Homo sapiens 112-117 8318951-7 1993 The present results specify the effects of differentiation inducers on the integrin profile of K562 cells and excludes the comprehension that TPA would induce expression of the platelet integrin alpha IIb on their surface. Tetradecanoylphorbol Acetate 142-145 integrin subunit alpha 2b Homo sapiens 186-204 7996872-4 1994 Hemin slightly increased the total hemoglobin production of the cells and phorbol diester (TPA), dimethyl sulfoxide (DMSO) and sodium butyrate (SB) increased the expression of megakaryocytic markers (gpIIb/IIIa complex). Tetradecanoylphorbol Acetate 91-94 integrin subunit alpha 2b Homo sapiens 200-205 7504152-2 1993 When HEL cells were treated with 10(-7) M TPA, glycophorin A expression and hemoglobin synthesis were reduced, while the expression of GP IIb/IIIa was induced in association with the morphological changes. Tetradecanoylphorbol Acetate 42-45 integrin subunit alpha 2b Homo sapiens 135-141 8359227-2 1993 A tumor promoter, TPA, induced a megakaryocyte marker, glycoprotein IIb/IIIa (GP IIb/IIIa) or IIIa (GP IIIa), but suppressed erythroid differentiation. Tetradecanoylphorbol Acetate 18-21 integrin subunit alpha 2b Homo sapiens 78-84 8325838-4 1993 Time- and calcium-dependent vesiculation of platelets in response to ADP, collagen, thrombin, phorbol myristate acetate, and the thrombin peptide SFLLRN were dramatically inhibited, in a concentration-dependent manner, by monoclonal antibodies to GPIIb-IIIa (A2A9, 7E3, PAC1) and RGDS. Tetradecanoylphorbol Acetate 94-119 integrin subunit alpha 2b Homo sapiens 247-252 8325838-7 1993 A central role for GPIIb-IIIa is supported by the near total inability of Glanzmann"s thrombasthenic (type I) platelets to vesiculate in response to thrombin, ADP, collagen, and phorbol 12-myristate 13-acetate. Tetradecanoylphorbol Acetate 178-209 integrin subunit alpha 2b Homo sapiens 19-24 1390239-9 1992 In two cases in which CD41 was not expressed before culture, the expression of CD41 was enhanced after culture with or without 12-O-tetradecanoyl-phorbol-13-acetate (TPA). Tetradecanoylphorbol Acetate 127-164 integrin subunit alpha 2b Homo sapiens 79-83 1390239-9 1992 In two cases in which CD41 was not expressed before culture, the expression of CD41 was enhanced after culture with or without 12-O-tetradecanoyl-phorbol-13-acetate (TPA). Tetradecanoylphorbol Acetate 166-169 integrin subunit alpha 2b Homo sapiens 79-83 35087488-3 2021 PMA-induced differentiation in these cells recapitulates steps of megakaryopoiesis including gene activation, expression of CD41/61 and CD61 platelet surface markers and accumulation of intracellular reactive oxygen species (ROS). Tetradecanoylphorbol Acetate 0-3 integrin subunit alpha 2b Homo sapiens 124-131 1515466-1 1992 A bispecific F(ab")2 monoclonal antibody which recognizes both the platelet GPIIb/IIIa receptor and human tissue plasminogen activator was produced to target tPA to platelets for enhancement of thrombolysis. Tetradecanoylphorbol Acetate 158-161 integrin subunit alpha 2b Homo sapiens 76-81 1893970-8 1991 CMK cells exhibited mRNA for GPIIb, and its expression was augmented by TPA addition, but not PF4. Tetradecanoylphorbol Acetate 72-75 integrin subunit alpha 2b Homo sapiens 29-34 1893970-9 1991 In contrast, CMK11-5 cells were found to contain mRNA for GPIIb and PF4, and their mRNA levels were increased by the addition of TPA. Tetradecanoylphorbol Acetate 129-132 integrin subunit alpha 2b Homo sapiens 58-63 2217443-11 1990 Among the megakaryocytic phenotypes, increase in GPIIb/IIIa complex, determined by a biochemical method, PPO by ultrastructural study, and the increase in cellular ploidy were clearly observed by PMA treatment. Tetradecanoylphorbol Acetate 196-199 integrin subunit alpha 2b Homo sapiens 49-54 6232432-6 1984 Immunoprecipitation, after ectolabeling of the cells with the C17 antibody and SDS-polyacrylamide gel electrophoresis, proved that TPA-induced K562 expressed both GP IIIa and GP IIb. Tetradecanoylphorbol Acetate 131-134 integrin subunit alpha 2b Homo sapiens 175-181 2665839-9 1989 On the other hand, PMA inhibited growth of CMK cells and induced most of them to express the GPIIb/IIIa antigen. Tetradecanoylphorbol Acetate 19-22 integrin subunit alpha 2b Homo sapiens 93-98 2455575-9 1988 An ultrastructural immunoperoxidase study demonstrated platelet GPIb and GPIIb/IIIa in both plasma membranes and protein synthesis areas such as perinuclear cisternae and endoplasmic reticulum after TPA induction. Tetradecanoylphorbol Acetate 199-202 integrin subunit alpha 2b Homo sapiens 73-78 2426709-5 1986 Promyeloid U937 cells could be induced by 4 beta-phorbol 12-myristate 13-acetate to synthesize and express GPIIb/IIIa-related molecules on their cell surface. Tetradecanoylphorbol Acetate 42-80 integrin subunit alpha 2b Homo sapiens 107-112 24637716-5 2014 Conversely, THAP11 overexpression accelerated the megakaryocytic differentiation induced by phorbol myristate acetate (PMA) with increased percentage of CD41+ cells, increased numbers of 4N cells, and elevated CD61 mRNA levels, and THAP11 knockdown attenuated the megakaryocytic differentiation. Tetradecanoylphorbol Acetate 119-122 integrin subunit alpha 2b Homo sapiens 153-157 24637716-5 2014 Conversely, THAP11 overexpression accelerated the megakaryocytic differentiation induced by phorbol myristate acetate (PMA) with increased percentage of CD41+ cells, increased numbers of 4N cells, and elevated CD61 mRNA levels, and THAP11 knockdown attenuated the megakaryocytic differentiation. Tetradecanoylphorbol Acetate 92-117 integrin subunit alpha 2b Homo sapiens 153-157 22336225-4 2012 Other indicators of PMA-induced HEL cell differentiation, such as increased expression of CD41/CD61 and an increase in cell complexity/granularity, were inhibited by cicaprost in an IP receptor-dependent and STAT3-dependent manner. Tetradecanoylphorbol Acetate 20-23 integrin subunit alpha 2b Homo sapiens 90-94 23297317-3 2013 In the present study, to investigate the effects of radiation on phorbol 12-myristate 13-acetate (PMA)-induced megakaryocytic differentiation of K562 cells, the cellular processes responsible for the expression of CD41 antigen (GPIIb/IIIa), which is reported to be expressed early in megakaryocyte maturation, were analyzed. Tetradecanoylphorbol Acetate 98-101 integrin subunit alpha 2b Homo sapiens 214-218 23297317-3 2013 In the present study, to investigate the effects of radiation on phorbol 12-myristate 13-acetate (PMA)-induced megakaryocytic differentiation of K562 cells, the cellular processes responsible for the expression of CD41 antigen (GPIIb/IIIa), which is reported to be expressed early in megakaryocyte maturation, were analyzed. Tetradecanoylphorbol Acetate 98-101 integrin subunit alpha 2b Homo sapiens 228-233 15190214-6 2004 Upon treatment with phorbol 12-myristate 13-acetate (PMA), human erythroleukemic cells (K562) underwent megakaryocytic differentiation characterized by polyploidization and expression of mature megakaryocyte surface markers such as CD41. Tetradecanoylphorbol Acetate 20-51 integrin subunit alpha 2b Homo sapiens 232-236 19302816-6 2009 After treatment of these two cell types with phorbol 12-myristate 13-acetate (PMA) for 48 h, cells with reduced Nm23-H1 expression had a higher percentage of 8N ploidy and higher expression of CD41 than K562 cells overexpressing Nm23-H1. Tetradecanoylphorbol Acetate 45-76 integrin subunit alpha 2b Homo sapiens 193-197 19302816-6 2009 After treatment of these two cell types with phorbol 12-myristate 13-acetate (PMA) for 48 h, cells with reduced Nm23-H1 expression had a higher percentage of 8N ploidy and higher expression of CD41 than K562 cells overexpressing Nm23-H1. Tetradecanoylphorbol Acetate 78-81 integrin subunit alpha 2b Homo sapiens 193-197 19031740-6 2008 Surface antigen Gp IIb-IIIa (CD41) of knock-down cells treated with phorbol 12-myristate 13-acetate was analyzed by flow cytometry. Tetradecanoylphorbol Acetate 68-99 integrin subunit alpha 2b Homo sapiens 29-33 20442406-4 2010 In this study, we report that ectopic expression of HA-PRMT1 in K562 cells suppressed phorbol 12-myristate 13-acetate (PMA)-induced megakaryocytic differentiation as demonstrated by changes in cytological characteristics, adhesive properties, and CD41 expression, whereas knockdown of PRMT1 by small interference RNA promoted differentiation. Tetradecanoylphorbol Acetate 86-117 integrin subunit alpha 2b Homo sapiens 247-251 20442406-4 2010 In this study, we report that ectopic expression of HA-PRMT1 in K562 cells suppressed phorbol 12-myristate 13-acetate (PMA)-induced megakaryocytic differentiation as demonstrated by changes in cytological characteristics, adhesive properties, and CD41 expression, whereas knockdown of PRMT1 by small interference RNA promoted differentiation. Tetradecanoylphorbol Acetate 119-122 integrin subunit alpha 2b Homo sapiens 247-251 17481561-1 2007 Phorbol-12-myristate-13-acetate (PMA) induces megakaryocytopoeisis in human erythroleukemia (HEL) cells which is characterized by the increase in cell size, increase in nuclear polyploidization and expression of megakaryocyte marker, CD41. Tetradecanoylphorbol Acetate 0-31 integrin subunit alpha 2b Homo sapiens 234-238 17481561-1 2007 Phorbol-12-myristate-13-acetate (PMA) induces megakaryocytopoeisis in human erythroleukemia (HEL) cells which is characterized by the increase in cell size, increase in nuclear polyploidization and expression of megakaryocyte marker, CD41. Tetradecanoylphorbol Acetate 33-36 integrin subunit alpha 2b Homo sapiens 234-238 15190214-6 2004 Upon treatment with phorbol 12-myristate 13-acetate (PMA), human erythroleukemic cells (K562) underwent megakaryocytic differentiation characterized by polyploidization and expression of mature megakaryocyte surface markers such as CD41. Tetradecanoylphorbol Acetate 53-56 integrin subunit alpha 2b Homo sapiens 232-236 12945873-5 2003 With 0.1 micromol/l phorbol-12-myristate-13-acetate, GPIIb/IIIa activation was irreversible. Tetradecanoylphorbol Acetate 20-51 integrin subunit alpha 2b Homo sapiens 53-58