PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 32780686-0 2020 Role of E2F1/SPHK1 and HSP27 During Irradiation in a PMA-Induced Inflammatory Model. Tetradecanoylphorbol Acetate 53-56 sphingosine kinase 1 Homo sapiens 13-18 33865856-5 2021 In addition, carbachol and PMA induced translocation of monomeric GFP-mSK1 to lamellipodia, while S1P induced translocation of dimeric GFP-mSK1 to filopodia, suggesting that SK1 regulates different cell biological processes dependent on dimerization. Tetradecanoylphorbol Acetate 27-30 sphingosine kinase 1 Homo sapiens 71-74 32780686-10 2020 Conclusions: E2F1 worked as a TF of SPHK1 and exhibited anti-inflammatory and antioxidative effects through SPHK1 in PMA-induced HaCaT cells after 625 nm PBM. Tetradecanoylphorbol Acetate 117-120 sphingosine kinase 1 Homo sapiens 108-113 32780686-6 2020 Results: A total of 6 TFs (e.g., E2F1) and 51 key DEGs (e.g., SPHK1) were identified after 625 nm PBM in PMA-stimulated HaCaT cells. Tetradecanoylphorbol Acetate 105-108 sphingosine kinase 1 Homo sapiens 62-67 30444039-6 2019 The inhibition of CerK reduced the phorbol 12-myristate 13-acetate-induced translocation of SphK1 to the plasma membrane (PM) and activation of the enzyme in membrane fractions of cells. Tetradecanoylphorbol Acetate 35-66 sphingosine kinase 1 Homo sapiens 92-97 30444039-9 2019 The mutation of four cationic amino acids to Ala in the 56-RRNHAR-61 domain in SphK1 reduced the phorbol 12-myristate 13-acetate- and C1P-induced translocation of SphK1 to the PM, however, the capacity of C1P to bind with and activate SphK1 was not affected by this mutation. Tetradecanoylphorbol Acetate 97-128 sphingosine kinase 1 Homo sapiens 79-84 22812190-0 2011 Inhibition of SPHK1 suppresses phorbol 12-myristate 13-acetate-induced metastatic phenotype in colorectal cancer HT-29 cells. Tetradecanoylphorbol Acetate 31-62 sphingosine kinase 1 Homo sapiens 14-19 30444039-9 2019 The mutation of four cationic amino acids to Ala in the 56-RRNHAR-61 domain in SphK1 reduced the phorbol 12-myristate 13-acetate- and C1P-induced translocation of SphK1 to the PM, however, the capacity of C1P to bind with and activate SphK1 was not affected by this mutation. Tetradecanoylphorbol Acetate 97-128 sphingosine kinase 1 Homo sapiens 163-168 30444039-9 2019 The mutation of four cationic amino acids to Ala in the 56-RRNHAR-61 domain in SphK1 reduced the phorbol 12-myristate 13-acetate- and C1P-induced translocation of SphK1 to the PM, however, the capacity of C1P to bind with and activate SphK1 was not affected by this mutation. Tetradecanoylphorbol Acetate 97-128 sphingosine kinase 1 Homo sapiens 163-168 24054007-2 2013 METHODS: Human colon cancer LoVo cells were divided into three groups: phorbol 12-myristate 13-acetate (PMA) was used to induce the activation of SphK1 in the PMA group, N,N-dimethylsphingosine (DMS) used to suppress the activity of SphK1 in DMS group, and the cells treated with equal amount of 0.9 % NaCl instead of drugs served as the control group. Tetradecanoylphorbol Acetate 71-102 sphingosine kinase 1 Homo sapiens 146-151 24054007-2 2013 METHODS: Human colon cancer LoVo cells were divided into three groups: phorbol 12-myristate 13-acetate (PMA) was used to induce the activation of SphK1 in the PMA group, N,N-dimethylsphingosine (DMS) used to suppress the activity of SphK1 in DMS group, and the cells treated with equal amount of 0.9 % NaCl instead of drugs served as the control group. Tetradecanoylphorbol Acetate 71-102 sphingosine kinase 1 Homo sapiens 233-238 24054007-2 2013 METHODS: Human colon cancer LoVo cells were divided into three groups: phorbol 12-myristate 13-acetate (PMA) was used to induce the activation of SphK1 in the PMA group, N,N-dimethylsphingosine (DMS) used to suppress the activity of SphK1 in DMS group, and the cells treated with equal amount of 0.9 % NaCl instead of drugs served as the control group. Tetradecanoylphorbol Acetate 104-107 sphingosine kinase 1 Homo sapiens 146-151 21575515-2 2011 METHODS: Phorbol 12-myristate 13-acetate (PMA) was used to induce the activity of SphK1 and N, N-dimethylsphingosine (DMS) was used to suppress the activity of SphK1. Tetradecanoylphorbol Acetate 9-40 sphingosine kinase 1 Homo sapiens 82-87 21575515-2 2011 METHODS: Phorbol 12-myristate 13-acetate (PMA) was used to induce the activity of SphK1 and N, N-dimethylsphingosine (DMS) was used to suppress the activity of SphK1. Tetradecanoylphorbol Acetate 42-45 sphingosine kinase 1 Homo sapiens 82-87 22812190-6 2011 PMA induced a metastatic phenotype in colorectal cancer cells, as indicated by cell viability, migration and invasion capacity, and ERK1/2 phosphorylation, whereas SPHK1 siRNA transfection suppressed the metastatic phenotype of tumor cells and antagonized PMA"s effects. Tetradecanoylphorbol Acetate 256-259 sphingosine kinase 1 Homo sapiens 164-169 22812190-8 2011 Suppression of SPHK1 expression suppresses the PMA-induced metastatic phenotype via ERK1/2 phosphorylation in human colorectal cancer cells. Tetradecanoylphorbol Acetate 47-50 sphingosine kinase 1 Homo sapiens 15-20 19914200-5 2010 Together the evidence indicates that the COOH-terminal region of SphK1 encompasses a structural element that is necessary for the increase in catalytic activity in response to PMA treatment and that its deletion renders SphK1 constitutively active with respect to PMA treatment. Tetradecanoylphorbol Acetate 264-267 sphingosine kinase 1 Homo sapiens 65-70 16571380-3 2006 A similar upregulation of SK-1 is also observed with the direct protein kinase C activator 12-O-tetradecanoylphorbol-13-acetate (TPA). Tetradecanoylphorbol Acetate 91-127 sphingosine kinase 1 Homo sapiens 26-30 19914200-3 2010 Herein, we report that deletion of 21 amino acids from the COOH-terminus of SphK1 (1-363) results in increased catalytic activity relative to wild-type SphK1 (1-384) which is independent of the phosphorylation state of Serine 225 and PMA stimulation. Tetradecanoylphorbol Acetate 234-237 sphingosine kinase 1 Homo sapiens 76-81 19914200-5 2010 Together the evidence indicates that the COOH-terminal region of SphK1 encompasses a structural element that is necessary for the increase in catalytic activity in response to PMA treatment and that its deletion renders SphK1 constitutively active with respect to PMA treatment. Tetradecanoylphorbol Acetate 176-179 sphingosine kinase 1 Homo sapiens 65-70 16571380-3 2006 A similar upregulation of SK-1 is also observed with the direct protein kinase C activator 12-O-tetradecanoylphorbol-13-acetate (TPA). Tetradecanoylphorbol Acetate 129-132 sphingosine kinase 1 Homo sapiens 26-30 16571380-6 2006 Moreover, the increased SK-1 mRNA expression results from an increased promoter activation by histamine and TPA. Tetradecanoylphorbol Acetate 108-111 sphingosine kinase 1 Homo sapiens 24-28 16571380-7 2006 In mechanistic terms, the transcriptional upregulation of SK-1 is dependent on PKC and the extracellular signal-regulated protein kinase (ERK) cascade since staurosporine and the MEK inhibitor U0126 abolish the TPA-induced SK-1 induction. Tetradecanoylphorbol Acetate 211-214 sphingosine kinase 1 Homo sapiens 58-62 16571380-9 2006 Parallel to the induction of SK-1, histamine and TPA stimulate an increased migration of endothelial cells, which is prevented by depletion of the SK-1 by small interfering RNA (siRNA). Tetradecanoylphorbol Acetate 49-52 sphingosine kinase 1 Homo sapiens 147-151 16571380-11 2006 Interestingly, only depletion of PKC-alpha leads to a complete loss of TPA- and histamine-triggered SK-1 induction and cell migration. Tetradecanoylphorbol Acetate 71-74 sphingosine kinase 1 Homo sapiens 100-104 14729073-1 2003 The prolonged treatment with phorbol 12-myristate 13-acetate (PMA) of a human megakaryoblastic leukemia cell line, MEG-O1, induced increase of sphingosine kinase (SPHK) enzyme activity and SPHK1 protein expression as well as SPHK1 message. Tetradecanoylphorbol Acetate 29-60 sphingosine kinase 1 Homo sapiens 189-194 14729073-1 2003 The prolonged treatment with phorbol 12-myristate 13-acetate (PMA) of a human megakaryoblastic leukemia cell line, MEG-O1, induced increase of sphingosine kinase (SPHK) enzyme activity and SPHK1 protein expression as well as SPHK1 message. Tetradecanoylphorbol Acetate 29-60 sphingosine kinase 1 Homo sapiens 225-230 14729073-1 2003 The prolonged treatment with phorbol 12-myristate 13-acetate (PMA) of a human megakaryoblastic leukemia cell line, MEG-O1, induced increase of sphingosine kinase (SPHK) enzyme activity and SPHK1 protein expression as well as SPHK1 message. Tetradecanoylphorbol Acetate 62-65 sphingosine kinase 1 Homo sapiens 143-161 14729073-1 2003 The prolonged treatment with phorbol 12-myristate 13-acetate (PMA) of a human megakaryoblastic leukemia cell line, MEG-O1, induced increase of sphingosine kinase (SPHK) enzyme activity and SPHK1 protein expression as well as SPHK1 message. Tetradecanoylphorbol Acetate 62-65 sphingosine kinase 1 Homo sapiens 163-167 14729073-1 2003 The prolonged treatment with phorbol 12-myristate 13-acetate (PMA) of a human megakaryoblastic leukemia cell line, MEG-O1, induced increase of sphingosine kinase (SPHK) enzyme activity and SPHK1 protein expression as well as SPHK1 message. Tetradecanoylphorbol Acetate 62-65 sphingosine kinase 1 Homo sapiens 189-194 14729073-1 2003 The prolonged treatment with phorbol 12-myristate 13-acetate (PMA) of a human megakaryoblastic leukemia cell line, MEG-O1, induced increase of sphingosine kinase (SPHK) enzyme activity and SPHK1 protein expression as well as SPHK1 message. Tetradecanoylphorbol Acetate 62-65 sphingosine kinase 1 Homo sapiens 225-230 14729073-2 2003 Protein kinase C (PKC) inhibitor prevented the PMA-induced SPHK1 gene expression. Tetradecanoylphorbol Acetate 47-50 sphingosine kinase 1 Homo sapiens 59-64 35489137-5 2022 For in vitro experiments, SphK1 in human SH-SY5Y neuroblastoma cells was activated using SphK1-specific activator phorbol 12-myristate 13-acetate (PMA). Tetradecanoylphorbol Acetate 114-145 sphingosine kinase 1 Homo sapiens 26-31 35489137-5 2022 For in vitro experiments, SphK1 in human SH-SY5Y neuroblastoma cells was activated using SphK1-specific activator phorbol 12-myristate 13-acetate (PMA). Tetradecanoylphorbol Acetate 114-145 sphingosine kinase 1 Homo sapiens 89-94 35489137-5 2022 For in vitro experiments, SphK1 in human SH-SY5Y neuroblastoma cells was activated using SphK1-specific activator phorbol 12-myristate 13-acetate (PMA). Tetradecanoylphorbol Acetate 147-150 sphingosine kinase 1 Homo sapiens 26-31 35489137-5 2022 For in vitro experiments, SphK1 in human SH-SY5Y neuroblastoma cells was activated using SphK1-specific activator phorbol 12-myristate 13-acetate (PMA). Tetradecanoylphorbol Acetate 147-150 sphingosine kinase 1 Homo sapiens 89-94 14729073-1 2003 The prolonged treatment with phorbol 12-myristate 13-acetate (PMA) of a human megakaryoblastic leukemia cell line, MEG-O1, induced increase of sphingosine kinase (SPHK) enzyme activity and SPHK1 protein expression as well as SPHK1 message. Tetradecanoylphorbol Acetate 29-60 sphingosine kinase 1 Homo sapiens 143-161 14729073-1 2003 The prolonged treatment with phorbol 12-myristate 13-acetate (PMA) of a human megakaryoblastic leukemia cell line, MEG-O1, induced increase of sphingosine kinase (SPHK) enzyme activity and SPHK1 protein expression as well as SPHK1 message. Tetradecanoylphorbol Acetate 29-60 sphingosine kinase 1 Homo sapiens 163-167 12124383-7 2002 PMA induced translocation of both endogenous and green fluorescent protein (GFP)-tagged human SK1 (hSK1) to the plasma membrane. Tetradecanoylphorbol Acetate 0-3 sphingosine kinase 1 Homo sapiens 94-97 12124383-7 2002 PMA induced translocation of both endogenous and green fluorescent protein (GFP)-tagged human SK1 (hSK1) to the plasma membrane. Tetradecanoylphorbol Acetate 0-3 sphingosine kinase 1 Homo sapiens 99-103 12124383-8 2002 PMA also induced phosphorylation of GFP-hSK1. Tetradecanoylphorbol Acetate 0-3 sphingosine kinase 1 Homo sapiens 40-44