PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 7554227-8 1995 To establish that TPA (PKC) regulates assembly of vimentin into the cytoskeleton of astrocytes, we used pulse-chase (20/5 min) labeling with [35S]methionine, and immunoprecipitations with an anti-vimentin mAb from extractable and cytoskeletal fractions. Tetradecanoylphorbol Acetate 18-21 vimentin Gallus gallus 50-58 7554227-9 1995 These studies revealed that 20 min treatment with TPA leads to a 3-fold increase in the rate of newly synthesized full-length vimentin assembly (posttranslational assembly). Tetradecanoylphorbol Acetate 50-53 vimentin Gallus gallus 126-134 7554227-10 1995 Furthermore, TPA increased cotranslational assembly of vimentin. Tetradecanoylphorbol Acetate 13-16 vimentin Gallus gallus 55-63 6354733-0 1983 TPA induces simultaneous alterations in the synthesis and organization of vimentin. Tetradecanoylphorbol Acetate 0-3 vimentin Gallus gallus 74-82 6354733-1 1983 We have found an increased rate of vimentin synthesis in TPA-treated chicken embryonic fibroblasts, as shown by two-dimensional electrophoretic separation of newly synthesized polypeptides. Tetradecanoylphorbol Acetate 57-60 vimentin Gallus gallus 35-43 6354733-3 1983 Treatment with TPA also altered the organization of the vimentin-containing intermediate filaments, as revealed by immunofluorescence. Tetradecanoylphorbol Acetate 15-18 vimentin Gallus gallus 56-64 6354733-4 1983 Treatments which inhibited the TPA-induced rearrangements of vimentin filaments did not prevent the increase in the rate of vimentin synthesis, indicating that gross alterations in cytoskeletal organization were not the immediate cause of the altered vimentin synthesis. Tetradecanoylphorbol Acetate 31-34 vimentin Gallus gallus 61-69 6354733-6 1983 TPA as a positive modulator of vimentin synthesis may serve as a useful probe for an eventual understanding of the dynamics of the mechanisms that control the assembly and organization of vimentin filaments. Tetradecanoylphorbol Acetate 0-3 vimentin Gallus gallus 31-39 6354733-6 1983 TPA as a positive modulator of vimentin synthesis may serve as a useful probe for an eventual understanding of the dynamics of the mechanisms that control the assembly and organization of vimentin filaments. Tetradecanoylphorbol Acetate 0-3 vimentin Gallus gallus 188-196