PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 2525477-2 1989 In I83 cells activated by a 1-h pulse of 12-O-tetradecanoylphorbol 13-acetate, the BSF-MP6-dependent DNA synthesis was strongly enhanced by 50-100 U/ml of recombinant IL 4. Tetradecanoylphorbol Acetate 41-77 interleukin 4 Homo sapiens 167-171 2535858-6 1989 The IL-4 gene is inducible after treatment of human T cell clone by phorbol-12-myristate-13-acetate (TPA) and calcium ionophore A23187. Tetradecanoylphorbol Acetate 68-99 interleukin 4 Homo sapiens 4-8 2467815-3 1989 IL 4 inhibits both the spontaneous and the phorbol myristate acetate (PMA)-induced hyperexpression of CD5 on tonsil B cells. Tetradecanoylphorbol Acetate 43-68 interleukin 4 Homo sapiens 0-4 2467815-3 1989 IL 4 inhibits both the spontaneous and the phorbol myristate acetate (PMA)-induced hyperexpression of CD5 on tonsil B cells. Tetradecanoylphorbol Acetate 70-73 interleukin 4 Homo sapiens 0-4 2467815-4 1989 In contrast, IL 4 only suppresses the PMA-induced hyperexpression of CD5 on B-CLL, whereas the spontaneous CD5 expression is essentially unaffected. Tetradecanoylphorbol Acetate 38-41 interleukin 4 Homo sapiens 13-17 2643668-11 1989 An approximately ten-fold greater level of IL-4 production was observed when they were stimulated with A23187 in combination with PMA. Tetradecanoylphorbol Acetate 130-133 interleukin 4 Homo sapiens 43-47 2783393-2 1989 IL-4 induced marked proliferation of thymocytes after PHA and TPA stimulation, in contrast to the marginal response of T cells from adult peripheral blood. Tetradecanoylphorbol Acetate 62-65 interleukin 4 Homo sapiens 0-4 2523864-1 1989 Recombinant IL-2 (rIL-2) and IL-4 (rIL-4) promote proliferation of human CD4+ T cells activated in the presence of PHA, TPA or OKT-3 monoclonal antibody (MAb), whereas the production of interferon-gamma (IFN) can be induced only by rIL-2. Tetradecanoylphorbol Acetate 120-123 interleukin 4 Homo sapiens 29-33 2535858-6 1989 The IL-4 gene is inducible after treatment of human T cell clone by phorbol-12-myristate-13-acetate (TPA) and calcium ionophore A23187. Tetradecanoylphorbol Acetate 101-104 interleukin 4 Homo sapiens 4-8 2535858-7 1989 The 2.3-kb 5"-flanking region of the human IL-4 gene transiently transfected into Jurkat human T cell leukemia cells is activated efficiently in response to TPA and A23187 stimulation and, although less efficiently, by human T cell leukemia virus type I-encoded p40x or BPV-encoded E2 protein. Tetradecanoylphorbol Acetate 157-160 interleukin 4 Homo sapiens 43-47 3126369-5 1988 B-CLL cells could become responsive with the inclusion of the phorbol ester TPA (12-O-tetradecanoylphorbol-13-acetate) as co-stimulant such that half of the populations were now activated by IL4, particularly when BCGF was also present. Tetradecanoylphorbol Acetate 76-79 interleukin 4 Homo sapiens 191-194 3126369-5 1988 B-CLL cells could become responsive with the inclusion of the phorbol ester TPA (12-O-tetradecanoylphorbol-13-acetate) as co-stimulant such that half of the populations were now activated by IL4, particularly when BCGF was also present. Tetradecanoylphorbol Acetate 81-117 interleukin 4 Homo sapiens 191-194 30566262-8 2019 Up-regulation of cytokines (TSLP, IL-4, IL-5, IL-13, RANTES) in human mast cells treated with phorbol 12-myristate 13-acetate and calcium ionophore was also suppressed by boehmite. Tetradecanoylphorbol Acetate 94-125 interleukin 4 Homo sapiens 34-38 31335245-1 2019 Context: We reported that D-4F, an apolipoprotein A-I (Apo A-I) mimetic polypeptide with 18 d-amino acids, suppressed IL-4 induced macrophage alternative activation and TGF-beta1 expression in phorbol 12-myristate 13-acetate (PMA) treated human acute monocytic leukemia cells (THP-1). Tetradecanoylphorbol Acetate 193-224 interleukin 4 Homo sapiens 118-122 31335245-1 2019 Context: We reported that D-4F, an apolipoprotein A-I (Apo A-I) mimetic polypeptide with 18 d-amino acids, suppressed IL-4 induced macrophage alternative activation and TGF-beta1 expression in phorbol 12-myristate 13-acetate (PMA) treated human acute monocytic leukemia cells (THP-1). Tetradecanoylphorbol Acetate 226-229 interleukin 4 Homo sapiens 118-122 28053321-3 2017 The functionality of these IL4 haplotypes in the response of immune cells to phorbol 12-myristate 13-acetate (PMA) with Ionomycin and IL-1beta (as inflammatory stimuli) was evaluated. Tetradecanoylphorbol Acetate 77-108 interleukin 4 Homo sapiens 27-30 30537802-7 2018 In CD4+ T cells treated with PMA+MLIF, the expression levels of IFN-gamma, TNF-alpha and IL-4 were strongly inhibited (p<0.001, p<0.001 and p<0.0094), compared to PMA treatment alone, for both, rhinitis and asthma. Tetradecanoylphorbol Acetate 29-32 interleukin 4 Homo sapiens 89-93 30537802-7 2018 In CD4+ T cells treated with PMA+MLIF, the expression levels of IFN-gamma, TNF-alpha and IL-4 were strongly inhibited (p<0.001, p<0.001 and p<0.0094), compared to PMA treatment alone, for both, rhinitis and asthma. Tetradecanoylphorbol Acetate 172-175 interleukin 4 Homo sapiens 89-93 28053321-3 2017 The functionality of these IL4 haplotypes in the response of immune cells to phorbol 12-myristate 13-acetate (PMA) with Ionomycin and IL-1beta (as inflammatory stimuli) was evaluated. Tetradecanoylphorbol Acetate 110-113 interleukin 4 Homo sapiens 27-30 25449852-5 2014 Circulating Th1, Th2 and Th17 effector cells were identified by intracellular staining for IFN-gamma, IL-4 and IL-17, respectively, upon in vitro stimulation with PMA and calcium ionophore. Tetradecanoylphorbol Acetate 163-166 interleukin 4 Homo sapiens 102-106 27119568-3 2016 In this study, we demonstrated that exposure of anti-CD3/CD28 or phorbol 12-myristate 13-acetate (PMA)/ionomycin-activated HuT-78 T lymphocyte cells to 10-20 mM d-lactate significantly increased IL-4 and IL-13 production. Tetradecanoylphorbol Acetate 65-96 interleukin 4 Homo sapiens 195-199 27119568-3 2016 In this study, we demonstrated that exposure of anti-CD3/CD28 or phorbol 12-myristate 13-acetate (PMA)/ionomycin-activated HuT-78 T lymphocyte cells to 10-20 mM d-lactate significantly increased IL-4 and IL-13 production. Tetradecanoylphorbol Acetate 98-101 interleukin 4 Homo sapiens 195-199 24831767-7 2014 RESULTS: Monocytes derived from peripheral blood mononuclear cells cultured in interleukin-4 and granulocyte macrophage colony stimulating factor differentiated into immature dendritic cells that phenotypically differentiated into mature dendritic cells in response to conditioned media from phorbol myristate acetate-activated THP-1 monocytes. Tetradecanoylphorbol Acetate 292-317 interleukin 4 Homo sapiens 79-92 22416475-5 2012 Th1/Th2 and Tc1/Tc2 were determined by analyzing intracellular cytokine staining for IFN-gamma and IL-4 in blood CD4+ and CD8+ T cells using flow cytometry after stimulation with PMA and ionomycin. Tetradecanoylphorbol Acetate 179-182 interleukin 4 Homo sapiens 99-103 22249152-4 2012 The kinetics of IL-4delta2 and IL-4 production by phorbol myristate acetate (PMA)/ionomycin-activated cells differed, with IL-4delta2 increasing at 48-72h and IL-4 peaking at 24h. Tetradecanoylphorbol Acetate 50-75 interleukin 4 Homo sapiens 26-35 22249152-4 2012 The kinetics of IL-4delta2 and IL-4 production by phorbol myristate acetate (PMA)/ionomycin-activated cells differed, with IL-4delta2 increasing at 48-72h and IL-4 peaking at 24h. Tetradecanoylphorbol Acetate 50-75 interleukin 4 Homo sapiens 16-20 22249152-4 2012 The kinetics of IL-4delta2 and IL-4 production by phorbol myristate acetate (PMA)/ionomycin-activated cells differed, with IL-4delta2 increasing at 48-72h and IL-4 peaking at 24h. Tetradecanoylphorbol Acetate 77-80 interleukin 4 Homo sapiens 26-35 22249152-4 2012 The kinetics of IL-4delta2 and IL-4 production by phorbol myristate acetate (PMA)/ionomycin-activated cells differed, with IL-4delta2 increasing at 48-72h and IL-4 peaking at 24h. Tetradecanoylphorbol Acetate 77-80 interleukin 4 Homo sapiens 16-20 22160839-4 2012 Th1 and Th2 cells, levels of INF-gamma, IL-2, IL-4 and the activities of T-bet and GATA3 were significantly increased after incubation with PMA and ionomycin. Tetradecanoylphorbol Acetate 140-143 interleukin 4 Homo sapiens 46-50 22160839-5 2012 However, the number of Th1 cells, Th1/ Th2, the levels of INF-gamma and INF-gamma/IL-4, and the activities and protein levels of T-bet and GATA3 were decreased after incubation with PMA and ionomycin in the presence of morphine. Tetradecanoylphorbol Acetate 182-185 interleukin 4 Homo sapiens 82-86 17662038-4 2008 There was a significant increase in the percentage of CD4+ and CD8+ T cells producing IL-4 and IL-13 and decrease in the percentage of CD4+ and CD8+ T cells producing IFN-gamma upon in vitro stimulation with phorbol 12-myristate 13-acetate and ionomycin in children with AD compared to healthy ones. Tetradecanoylphorbol Acetate 208-239 interleukin 4 Homo sapiens 86-90 21966389-4 2011 Potentiation of anti-IgE- induced and calcium ionophore/PMA-induced degranulation was augmented in mast cells cultured with IL-4, and this effect was reduced or abolished by pre-treatment with A(2B)siRNA and selective A(2B) receptor antagonists, respectively. Tetradecanoylphorbol Acetate 56-59 interleukin 4 Homo sapiens 124-128 19420081-8 2009 After stimulation with phorbol myristate acetate-ionomycin, high gamma interferon and low IL-4 levels were detected. Tetradecanoylphorbol Acetate 23-48 interleukin 4 Homo sapiens 90-94 21683432-3 2011 METHODS: Mitogen (concanavalin A/phorbol 12-myristate 13-acetate)-stimulated IL-4, IL-5, IL-13, and IFN-gamma levels were measured in supernatants from cord blood mononuclear cells and PBMCs at birth, 3 months, and 12 months. Tetradecanoylphorbol Acetate 33-64 interleukin 4 Homo sapiens 77-81 20354949-7 2010 Moreover, acteoside inhibited histamine release, TNF- alpha, and IL-4 production in a dose-dependent manner from calcium ionophore A23187 plus phorbol 12-myristate 13-acetate (PMA) or compound 48/80-stimulated KU812 cells. Tetradecanoylphorbol Acetate 176-179 interleukin 4 Homo sapiens 65-69 18691343-2 2009 Intracellular interleukin-4 (Th2 cytokine) and interferon-gamma (Th1 cytokine) production was assessed in CD4+ T lymphocytes activated by phorbol 12-myristate 13-acetate and ionomycin using flow cytometry. Tetradecanoylphorbol Acetate 138-169 interleukin 4 Homo sapiens 14-27 18684225-3 2008 Concanavalin A induced expression of IL-2 mRNA and production of IL-2, and the combination of thapsigargin and phorbol myristate acetate induced expression of IL-4 and IL-10 mRNAs and production of IL-4 and IL-10. Tetradecanoylphorbol Acetate 111-136 interleukin 4 Homo sapiens 159-163 18684225-3 2008 Concanavalin A induced expression of IL-2 mRNA and production of IL-2, and the combination of thapsigargin and phorbol myristate acetate induced expression of IL-4 and IL-10 mRNAs and production of IL-4 and IL-10. Tetradecanoylphorbol Acetate 111-136 interleukin 4 Homo sapiens 198-202 18325701-7 2008 RESULTS: HcFs inhibited the expression of IL-4 and IL-5 in response to phorbol 12-myristate 13-acetate (PMA) and calcium ionophore (CaI) in Jurkat T cells and the human mast cell line, HMC-1. Tetradecanoylphorbol Acetate 71-102 interleukin 4 Homo sapiens 42-46 18325701-7 2008 RESULTS: HcFs inhibited the expression of IL-4 and IL-5 in response to phorbol 12-myristate 13-acetate (PMA) and calcium ionophore (CaI) in Jurkat T cells and the human mast cell line, HMC-1. Tetradecanoylphorbol Acetate 104-107 interleukin 4 Homo sapiens 42-46 18054415-6 2007 Intracellular expression of IFN-gamma and IL-4 by CD3+CD8(-) (Th1 and Th2, respectively) and CD3+CD8+ (Tc1 and Tc2, respectively) was estimated by flow cytometry in peripheral blood cells after stimulation with PMA and ionomycin. Tetradecanoylphorbol Acetate 211-214 interleukin 4 Homo sapiens 42-46 18207029-4 2008 Culture in the presence of TNF-alpha induced some differentiation, but only treatment with PMA and ionomycin (with or without prior culture in GM-CSF and IL-4) induced morphological and phenotypic changes consistent with DC-like maturation, and even these maximally differentiated KG-1 cells showed lower levels of surface marker expression, macromolecular endocytosis, and ability to stimulate in allogeneic MLR compared with in vitro monocyte-derived DCs. Tetradecanoylphorbol Acetate 91-94 interleukin 4 Homo sapiens 154-158 15654514-4 2004 RESULTS: HMC-1 produced substantial amounts of GM-CSF and IL-8 and smaller amounts of TNF-alpha, IL-4 and IL-6 after being stimulated with PMA together with A23187. Tetradecanoylphorbol Acetate 139-142 interleukin 4 Homo sapiens 97-101 16125466-3 2005 The proportion of CD4+ and CD8+ T cells that produced IFN-gamma and IL-4 after stimulation with PMA (Phorbol 12-myristate 13-acetate) and ionomycin was significantly reduced in VL patients compared to sub-clinical and asymptomatic infections or healthy controls. Tetradecanoylphorbol Acetate 96-99 interleukin 4 Homo sapiens 68-72 16125466-3 2005 The proportion of CD4+ and CD8+ T cells that produced IFN-gamma and IL-4 after stimulation with PMA (Phorbol 12-myristate 13-acetate) and ionomycin was significantly reduced in VL patients compared to sub-clinical and asymptomatic infections or healthy controls. Tetradecanoylphorbol Acetate 101-132 interleukin 4 Homo sapiens 68-72 15855253-4 2005 Intracellular expression of IFNgamma and IL-4 was evaluated by multicolor flow-cytometry analysis in peripheral lymphocytes in vitro stimulated by phorbol-12-myristate-13-acetate (PMA) (25 ng/ml) and ionomycin (1 mug/ml) in presence of monensin (5 microm). Tetradecanoylphorbol Acetate 147-178 interleukin 4 Homo sapiens 41-45 15855253-4 2005 Intracellular expression of IFNgamma and IL-4 was evaluated by multicolor flow-cytometry analysis in peripheral lymphocytes in vitro stimulated by phorbol-12-myristate-13-acetate (PMA) (25 ng/ml) and ionomycin (1 mug/ml) in presence of monensin (5 microm). Tetradecanoylphorbol Acetate 180-183 interleukin 4 Homo sapiens 41-45 15778356-4 2005 In contrast, IL-4-cultured NK cells produced significant levels of TNF-alpha and GM-CSF only when stimulated with PMA and ionomycin. Tetradecanoylphorbol Acetate 114-117 interleukin 4 Homo sapiens 13-17 17079162-4 2007 Fisetin decreased phorbol-12-myristate 13-acetate plus calcium ionophore A23187 (PMACI)-stimulated gene expression and production of tumor necrosis factor-alpha (TNF-alpha), interleukin (IL)-1beta, IL-4, IL-6, and IL-8 in HMC-1 cells. Tetradecanoylphorbol Acetate 18-49 interleukin 4 Homo sapiens 198-202 17200786-0 2007 IL-4 modulates tissue factor expression by human B lymphocytes in response to phorbol myristate acetate. Tetradecanoylphorbol Acetate 78-103 interleukin 4 Homo sapiens 0-4 16143405-7 2006 Upon activation with PMA and ionomycin, the purified CD4+CCR4+ T lymphocytes produced IL-4 and no IFN-gamma. Tetradecanoylphorbol Acetate 21-24 interleukin 4 Homo sapiens 86-90 15969673-9 2005 Furthermore, phorbol-12-myristate-13-acetate and calcium ionophore stimulated the release of IL-4 after 48 h treatment in the presence of anti-IL-4 receptor antibody. Tetradecanoylphorbol Acetate 13-44 interleukin 4 Homo sapiens 93-97 15969673-9 2005 Furthermore, phorbol-12-myristate-13-acetate and calcium ionophore stimulated the release of IL-4 after 48 h treatment in the presence of anti-IL-4 receptor antibody. Tetradecanoylphorbol Acetate 13-44 interleukin 4 Homo sapiens 143-147 15849849-4 2005 In vitro IFN-gamma and IL-4 secretion by peripheral blood mononuclear cells after stimulation with ionomycin and phorbol 12 myristate 13 acetate was measured by enzyme-linked immunosorbent assay (ELISA) tests. Tetradecanoylphorbol Acetate 113-144 interleukin 4 Homo sapiens 23-27 15792026-5 2004 The intracellular expression of IL-4 and IFN-gamma in CD4+ or CD8+ cells after stimulation with ionomycin/PMA was estimated by flow cytometer (FACSCalibur, Becton Dickinson) and serum levels of both cytokines were assessed with ELISA (R & D Systems) in all subjects. Tetradecanoylphorbol Acetate 106-109 interleukin 4 Homo sapiens 32-36 14994387-4 2004 IFN-gamma and IL-4 production by CD4+ T cells stimulated with phorbol myristate acetate (PMA) and calcium ionophore A23187 was measured by intracellular cytokine staining and flow cytometry. Tetradecanoylphorbol Acetate 62-87 interleukin 4 Homo sapiens 14-18 14994387-4 2004 IFN-gamma and IL-4 production by CD4+ T cells stimulated with phorbol myristate acetate (PMA) and calcium ionophore A23187 was measured by intracellular cytokine staining and flow cytometry. Tetradecanoylphorbol Acetate 89-92 interleukin 4 Homo sapiens 14-18 15067204-4 2004 Intracellular staining of interleukin-4 (IL-4) and interferon-gamma (IFN-gamma) production in CD4+ lymphocytes after phorbol myristate acetate and ionomycin stimulation was performed at the same time points. Tetradecanoylphorbol Acetate 117-142 interleukin 4 Homo sapiens 26-39 15556684-3 2004 Using enzyme-linked immunosorbent assay, we measured unstimulated and concanavalin A/phorbol myristate acetate-stimulated production of interleukin-4 (IL-4), IL-5, IL-10, IL-13 and interferon- gamma (IFN-gamma) by decidual explants from 59 healthy women delivered by unlabored cesarean section and from corresponding CBMCs in 39 of the 59. Tetradecanoylphorbol Acetate 85-110 interleukin 4 Homo sapiens 136-149 15556684-3 2004 Using enzyme-linked immunosorbent assay, we measured unstimulated and concanavalin A/phorbol myristate acetate-stimulated production of interleukin-4 (IL-4), IL-5, IL-10, IL-13 and interferon- gamma (IFN-gamma) by decidual explants from 59 healthy women delivered by unlabored cesarean section and from corresponding CBMCs in 39 of the 59. Tetradecanoylphorbol Acetate 85-110 interleukin 4 Homo sapiens 151-155 15320911-4 2004 Intracellular expression of interferon (IFN)-gamma, interleukin (IL)-2 and IL-4 in PMA/ionomycin-stimulated peripheral blood mononuclear cells (PBMC) was used to evaluate T helper frequencies. Tetradecanoylphorbol Acetate 83-86 interleukin 4 Homo sapiens 75-79 15153483-8 2004 The T(CM) were capable of 20-40 mean population doublings in vitro, and the expanded cells produced IFN-gamma, IL-2, and TNF-alpha in response to Ag, and a subset of cells also secreted IL-4 with PMA/ionomycin treatment. Tetradecanoylphorbol Acetate 196-199 interleukin 4 Homo sapiens 186-190 12794411-8 2003 After phorbol 12-myristate 13-acetate stimulation, the number of IL-4-producing CD4+ cells increased three-fold in the trauma patients compared with healthy control subjects. Tetradecanoylphorbol Acetate 6-37 interleukin 4 Homo sapiens 65-69 12950238-3 2003 In this study, the intracellular interleukin-4 and interferon-gamma production in CD4+ T-lymphocytes activated by phorbol 12-myristate 13-acetate and ionomycin was assessed via flow cytometry in order to determine the clinical significance of the Th1/Th2 ratio in 42 patients with ITP. Tetradecanoylphorbol Acetate 114-145 interleukin 4 Homo sapiens 33-46 12167345-3 2002 After stimulation with phorbol 12-myristate 13-acetate and ionomycin, an increase in the percentage of IFN-gamma and IL-4 producing CD8(+) T cells was observed during aging. Tetradecanoylphorbol Acetate 23-54 interleukin 4 Homo sapiens 117-121 12133875-7 2002 Intracellular expression of IFN-gamma, IL-2, and IL-4 was detected in CD4(+) and CD8(+) T cells after stimulation with phorbol 12-myristate 13-acetate and ionomycin. Tetradecanoylphorbol Acetate 119-150 interleukin 4 Homo sapiens 49-53 12473063-5 2002 Furthermore, in supernatants from whole blood samples stimulated with phorbol 12-myristate 13-acetate and ionomycin, production of IFN-gamma was significantly decreased, while IL-4 production remained unchanged in AD patients compared with healthy controls. Tetradecanoylphorbol Acetate 70-101 interleukin 4 Homo sapiens 176-180 11886168-2 2002 In this study, IL-4 did not induce virus production, but inhibited phorbol esters (PMA)-stimulated HIV expression in chronically infected promonocytic U1 cells. Tetradecanoylphorbol Acetate 83-86 interleukin 4 Homo sapiens 15-19 11726648-6 2002 Using human monocytes and T lymphocytes from same donors, we have found that monocyte 12/15-LO products mediate the indirect inhibitory effect of IL-4 on anti-CD3- or phytohemagglutinin/phorbol 12-myristate 13-acetate-stimulated IL-2 production by T lymphocytes. Tetradecanoylphorbol Acetate 186-217 interleukin 4 Homo sapiens 146-150 12402613-14 2002 When cells were stimulated by ionomicin and phorbol 12-myristate 13-acetate (PMA), more IFN-gamma appeared and high levels of IL-4 persisted in 75% of the samples, which looked like intent to correct the TH2 immunodeviation toward TH0. Tetradecanoylphorbol Acetate 44-75 interleukin 4 Homo sapiens 126-130 12402613-14 2002 When cells were stimulated by ionomicin and phorbol 12-myristate 13-acetate (PMA), more IFN-gamma appeared and high levels of IL-4 persisted in 75% of the samples, which looked like intent to correct the TH2 immunodeviation toward TH0. Tetradecanoylphorbol Acetate 77-80 interleukin 4 Homo sapiens 126-130 11678905-4 2001 Following re-stimulation in vitro with PMA and ionomycin, CD4+ T cells produced IFNgamma, TNFalpha, TNFbeta, IL-2, IL-4, IL-10 and IL-13. Tetradecanoylphorbol Acetate 39-42 interleukin 4 Homo sapiens 115-119 11781358-5 2002 Whereas a biased Th1 to Th2 cytokine profile has been suggested to underlie the pathogenesis of IMD in both species, we found defective production of IFN-gamma in our patients after in vitro stimulation of their PBMCs by phorbol-myristate acetate and ionomycin and both IFN-gamma and IL-4 deficiencies in V(alpha)24(+) NK T-enriched cells. Tetradecanoylphorbol Acetate 221-246 interleukin 4 Homo sapiens 284-288 11693999-1 2001 In this study, we report on the interferon-gamma (IFN-gamma) and interleukin-4 (IL-4) cytokine responses to phorbol myristate acetate (PMA) + ionomycin-stimulated CD3+ lymphocytes in asthmatic subjects when compared with normal donors. Tetradecanoylphorbol Acetate 108-133 interleukin 4 Homo sapiens 65-78 11693999-1 2001 In this study, we report on the interferon-gamma (IFN-gamma) and interleukin-4 (IL-4) cytokine responses to phorbol myristate acetate (PMA) + ionomycin-stimulated CD3+ lymphocytes in asthmatic subjects when compared with normal donors. Tetradecanoylphorbol Acetate 108-133 interleukin 4 Homo sapiens 80-84 11693999-1 2001 In this study, we report on the interferon-gamma (IFN-gamma) and interleukin-4 (IL-4) cytokine responses to phorbol myristate acetate (PMA) + ionomycin-stimulated CD3+ lymphocytes in asthmatic subjects when compared with normal donors. Tetradecanoylphorbol Acetate 135-138 interleukin 4 Homo sapiens 65-78 11693999-1 2001 In this study, we report on the interferon-gamma (IFN-gamma) and interleukin-4 (IL-4) cytokine responses to phorbol myristate acetate (PMA) + ionomycin-stimulated CD3+ lymphocytes in asthmatic subjects when compared with normal donors. Tetradecanoylphorbol Acetate 135-138 interleukin 4 Homo sapiens 80-84 11094642-5 2000 In addition to these phenotypical changes, IL-4 primed the phorbol-12-myristate-13-acetate (PMA)-induced luminol-dependent chemiluminescence response (LDCL) by normal human monocytes; this priming effect was abrogated in the presence of Lyprinol, or of BW B70C. Tetradecanoylphorbol Acetate 59-90 interleukin 4 Homo sapiens 43-47 11384880-11 2001 Furthermore, we showed that the PKC activator phorbol-12-myristate-13-acetate (PMA) also potentiated the IL-4-induced 3beta-HSD activity, thus suggesting that one signaling molecule that is involved in the signal transduction of the IL-4 action on 3beta-HSD type 1 expression is also a substrate for PKC. Tetradecanoylphorbol Acetate 46-77 interleukin 4 Homo sapiens 105-109 11384880-11 2001 Furthermore, we showed that the PKC activator phorbol-12-myristate-13-acetate (PMA) also potentiated the IL-4-induced 3beta-HSD activity, thus suggesting that one signaling molecule that is involved in the signal transduction of the IL-4 action on 3beta-HSD type 1 expression is also a substrate for PKC. Tetradecanoylphorbol Acetate 46-77 interleukin 4 Homo sapiens 233-237 11384880-11 2001 Furthermore, we showed that the PKC activator phorbol-12-myristate-13-acetate (PMA) also potentiated the IL-4-induced 3beta-HSD activity, thus suggesting that one signaling molecule that is involved in the signal transduction of the IL-4 action on 3beta-HSD type 1 expression is also a substrate for PKC. Tetradecanoylphorbol Acetate 79-82 interleukin 4 Homo sapiens 105-109 11384880-11 2001 Furthermore, we showed that the PKC activator phorbol-12-myristate-13-acetate (PMA) also potentiated the IL-4-induced 3beta-HSD activity, thus suggesting that one signaling molecule that is involved in the signal transduction of the IL-4 action on 3beta-HSD type 1 expression is also a substrate for PKC. Tetradecanoylphorbol Acetate 79-82 interleukin 4 Homo sapiens 233-237 11174192-10 2001 Ionomycin induced IL-4 secretion by BAL basophils, and this response was reduced with the addition of phorbol myristate acetate. Tetradecanoylphorbol Acetate 102-127 interleukin 4 Homo sapiens 18-22 11174192-11 2001 In contrast, phorbol myristate acetate promoted the secretion of IL-4 by BAL cells enriched for lymphocytes; both findings are identical to those reported for basophils and lymphocytes purified from blood. Tetradecanoylphorbol Acetate 13-38 interleukin 4 Homo sapiens 65-69 12539551-4 2001 The expression of IL-4 and IL-5 mRNA and protein of asthmatic T lymphocytes stimulated with PMA was significantly higher than that of asthmatic T lymphocytes stimulated without PMA respectively (P < 0.01) and that of normal T lymphocytes stimulated with PMA respectively (P < 0.01). Tetradecanoylphorbol Acetate 92-95 interleukin 4 Homo sapiens 18-22 12539551-4 2001 The expression of IL-4 and IL-5 mRNA and protein of asthmatic T lymphocytes stimulated with PMA was significantly higher than that of asthmatic T lymphocytes stimulated without PMA respectively (P < 0.01) and that of normal T lymphocytes stimulated with PMA respectively (P < 0.01). Tetradecanoylphorbol Acetate 177-180 interleukin 4 Homo sapiens 18-22 12539551-4 2001 The expression of IL-4 and IL-5 mRNA and protein of asthmatic T lymphocytes stimulated with PMA was significantly higher than that of asthmatic T lymphocytes stimulated without PMA respectively (P < 0.01) and that of normal T lymphocytes stimulated with PMA respectively (P < 0.01). Tetradecanoylphorbol Acetate 177-180 interleukin 4 Homo sapiens 18-22 12539551-5 2001 The expression of IL-4 and IL-5 mRNA and protein of asthmatic T lymphocytes stimulated with PMA and Ro31-8220 was significantly lower than that of asthmatic T lymphocytes stimulated only with PMA respectively (P < 0.01). Tetradecanoylphorbol Acetate 92-95 interleukin 4 Homo sapiens 18-22 11094642-5 2000 In addition to these phenotypical changes, IL-4 primed the phorbol-12-myristate-13-acetate (PMA)-induced luminol-dependent chemiluminescence response (LDCL) by normal human monocytes; this priming effect was abrogated in the presence of Lyprinol, or of BW B70C. Tetradecanoylphorbol Acetate 92-95 interleukin 4 Homo sapiens 43-47 10735602-7 2000 However, by in vitro activation with phorbol 12-myristate 13-acetate (PMA) and ionomycin, peripheral CD4 cells demonstrated a significant decrease of IFN-gamma-producing T helper 1 (Th1) cells and an increase of IL-4-producing T helper 2 (Th2) cells after immunotherapy. Tetradecanoylphorbol Acetate 37-68 interleukin 4 Homo sapiens 212-216 11074886-14 2000 Release of IL-2 and IL-4 from peripheral blood mononuclear cell fractions stimulated with phorbolmyristateacetate and ionomycin was significantly increased in patients with trauma but not from those stimulated with toxic shock syndrome toxin-1. Tetradecanoylphorbol Acetate 90-113 interleukin 4 Homo sapiens 20-24 10808178-6 2000 Although the majority of lymphocytes remained in the peritoneum, significant numbers of T lymphocytes were located in the spleen, where human IL-4, IL-5, and IFN-gamma messenger RNA expression was detected after stimulation with PHA and phorbol myristate acetate. Tetradecanoylphorbol Acetate 237-262 interleukin 4 Homo sapiens 142-146 10735602-7 2000 However, by in vitro activation with phorbol 12-myristate 13-acetate (PMA) and ionomycin, peripheral CD4 cells demonstrated a significant decrease of IFN-gamma-producing T helper 1 (Th1) cells and an increase of IL-4-producing T helper 2 (Th2) cells after immunotherapy. Tetradecanoylphorbol Acetate 70-73 interleukin 4 Homo sapiens 212-216 10674396-14 2000 The potential role of PKC was suggested by the observation that the well known PKC activator phorbol-12-myristate-13-acetate (PMA) potentiated the IL-4-induced 3beta-HSD activity. Tetradecanoylphorbol Acetate 93-124 interleukin 4 Homo sapiens 147-151 10674396-14 2000 The potential role of PKC was suggested by the observation that the well known PKC activator phorbol-12-myristate-13-acetate (PMA) potentiated the IL-4-induced 3beta-HSD activity. Tetradecanoylphorbol Acetate 126-129 interleukin 4 Homo sapiens 147-151 10520184-7 1999 The production of IFN-gamma and IL-4 following CD3-CD28 stimulation of RA PB MNC correlated significantly in a ratio 1 : 1 with production following ionomycin-PMA stimulation. Tetradecanoylphorbol Acetate 159-162 interleukin 4 Homo sapiens 32-36 9150836-4 1997 Only one neonate of group B showed detectable in vitro synthesis of IL4 (45 pg/ml) after PHA + TPA stimulation. Tetradecanoylphorbol Acetate 95-98 interleukin 4 Homo sapiens 68-71 9847438-5 1998 For this purpose, human lymphocytes were induced to express IL-4Ralpha chain by means of protein kinase C (PKC) activation with phorbol myristate acetate (PMA) or by triggering the Janus kinase-Stat pathway with IL-4 in the presence or absence of pharmacologic doses of dexamethasone. Tetradecanoylphorbol Acetate 128-153 interleukin 4 Homo sapiens 60-64 9847438-5 1998 For this purpose, human lymphocytes were induced to express IL-4Ralpha chain by means of protein kinase C (PKC) activation with phorbol myristate acetate (PMA) or by triggering the Janus kinase-Stat pathway with IL-4 in the presence or absence of pharmacologic doses of dexamethasone. Tetradecanoylphorbol Acetate 155-158 interleukin 4 Homo sapiens 60-64 9856820-5 1998 Increased production of IL-4 and IL-13 in both CD4+ CD45RO+ T cells and CD8+ CD45RO+ T cells after 4 h in vitro stimulation with phorbol 12-myristate 13-acetate and ionomycin, was more prominent in AD-H patients than in AD-L patients or healthy controls, whereas IFN-gamma-producing CD4+ CD45RO+ T cells and CD8+ CD45RO+ T cells were relatively diminished in AD-H patients. Tetradecanoylphorbol Acetate 129-160 interleukin 4 Homo sapiens 24-28 9856820-6 1998 CD4+ T cells and CD8 + T cells from AD-H patients, cultured for 48 h with phorbol 12-myristate 13-acetate and ionomycin, released larger amounts of IL-4 and IL-13 but smaller amounts of IFN-gamma than both types of cells from AD-L patients or healthy controls. Tetradecanoylphorbol Acetate 74-105 interleukin 4 Homo sapiens 148-152 9542602-3 1998 In most patients, we found reduced frequencies of T cells recalled to express CD69 and the cytokines interleukin (IL)-4 and interferon-gamma (IFN-gamma) after stimulation of peripheral blood mononuclear cells with phorbol 12-myristate 13-acetate (PMA) and ionomycin, as compared with normal donors. Tetradecanoylphorbol Acetate 214-245 interleukin 4 Homo sapiens 101-119 9503684-5 1997 Furthermore, the frequency of T cells which produced IL-4, IL-5 and IFN-gamma stimulated with phorbol myristate acetate and ionomycin increased and reduced in parallel with MFI of EG2-positive cells. Tetradecanoylphorbol Acetate 94-119 interleukin 4 Homo sapiens 53-57 9355960-7 1997 The production of IL-4 and IgE was enhanced in the cells treated with 12-O-tetradecanoyl phorbol-13-acetate. Tetradecanoylphorbol Acetate 70-107 interleukin 4 Homo sapiens 18-22 10388538-8 1999 These results suggest that the inhibition of PMA-induced HA adhesion by IFN-gamma and IL-4 may influence B cell migration through their ability to downregulate CD44-HA interactions. Tetradecanoylphorbol Acetate 45-48 interleukin 4 Homo sapiens 86-90 10191628-4 1999 Flow cytometric detection of intracellular cytokines in tonsillar mononuclear cells stimulated with PMA and ionomycin revealed that CD3 cells produced IL-1 alpha, IL-2, IL-4, IL-8, IFN-gamma and TNF-alpha, and CD19 cells produced IL-1 alpha, IL-6, IL-8 and TFN-alpha. Tetradecanoylphorbol Acetate 100-103 interleukin 4 Homo sapiens 169-173 9620661-3 1998 In fact, the secretion of IL-4 by basophils stimulated with ionomycin alone was down-regulated (30-70%) with the simultaneous addition of PMA. Tetradecanoylphorbol Acetate 138-141 interleukin 4 Homo sapiens 26-30 9620661-4 1998 In peripheral blood lymphocytes (PBL), however, the combination of ionomycin and PMA were highly synergistic, resulting in maximum IL-4 release but at a slower rate. Tetradecanoylphorbol Acetate 81-84 interleukin 4 Homo sapiens 131-135 9158101-4 1997 Upon stimulation of KU812 cells with either phorbol myristate acetate (PMA) or ionomycin (Iono), IL-4, but not IL-13, was produced in response to Iono, while IL-13, but not IL-4, was inducible by PMA. Tetradecanoylphorbol Acetate 44-69 interleukin 4 Homo sapiens 97-101 9158101-4 1997 Upon stimulation of KU812 cells with either phorbol myristate acetate (PMA) or ionomycin (Iono), IL-4, but not IL-13, was produced in response to Iono, while IL-13, but not IL-4, was inducible by PMA. Tetradecanoylphorbol Acetate 71-74 interleukin 4 Homo sapiens 97-101 9158101-4 1997 Upon stimulation of KU812 cells with either phorbol myristate acetate (PMA) or ionomycin (Iono), IL-4, but not IL-13, was produced in response to Iono, while IL-13, but not IL-4, was inducible by PMA. Tetradecanoylphorbol Acetate 196-199 interleukin 4 Homo sapiens 97-101 8774354-5 1996 In addition to these phenotypical changes, IL-4 primed the phorbol-12-myristate-13-acetate (PMA)-induced luminol-dependent chemiluminescence response (LDCL) by normal human monocytes, this priming effect being abrogated in the presence of BW B70C. Tetradecanoylphorbol Acetate 59-90 interleukin 4 Homo sapiens 43-47 8977524-7 1996 A23187, which synergizes with PMA in the induction of IL-4 and IFN-gamma, inhibited PMA-induced IL-10 production in a dose-dependent manner. Tetradecanoylphorbol Acetate 30-33 interleukin 4 Homo sapiens 54-58 8977524-7 1996 A23187, which synergizes with PMA in the induction of IL-4 and IFN-gamma, inhibited PMA-induced IL-10 production in a dose-dependent manner. Tetradecanoylphorbol Acetate 84-87 interleukin 4 Homo sapiens 54-58 8871052-6 1996 In three out of nine clones tested, the stimulation with anti-CD2/CD28/phorbol myristate acetate (PMA) induced a shift of the IFN-gamma/IL-4 ratio towards a Th2-type cytokine profile. Tetradecanoylphorbol Acetate 71-96 interleukin 4 Homo sapiens 136-140 8871052-6 1996 In three out of nine clones tested, the stimulation with anti-CD2/CD28/phorbol myristate acetate (PMA) induced a shift of the IFN-gamma/IL-4 ratio towards a Th2-type cytokine profile. Tetradecanoylphorbol Acetate 98-101 interleukin 4 Homo sapiens 136-140 8774354-5 1996 In addition to these phenotypical changes, IL-4 primed the phorbol-12-myristate-13-acetate (PMA)-induced luminol-dependent chemiluminescence response (LDCL) by normal human monocytes, this priming effect being abrogated in the presence of BW B70C. Tetradecanoylphorbol Acetate 92-95 interleukin 4 Homo sapiens 43-47 7560644-2 1995 The proportions of TCCs from patients with AD producing interleukin-4 in response to stimulation with phorbol 12-myristate 13-acetate plus anti-CD3 antibody were higher, whereas the proportions of interferon-gamma--producing TCCs were lower than those of control subjects. Tetradecanoylphorbol Acetate 102-133 interleukin 4 Homo sapiens 56-69 8625537-9 1996 Similar but transient inhibition of most T-cell products (IL-2, IL-3, IL-4, IL-5, IL-10, TNF-beta and GM-CSF) was noted in the PMA/ionomycin-containing cultures. Tetradecanoylphorbol Acetate 127-130 interleukin 4 Homo sapiens 70-74 8543752-4 1995 The release of IL-4 and IFN-gamma from peripheral blood mononuclear cells stimulated by polyclonal agents (calcium ionophore A23187 and phorbol myristate acetate) was measured by ELISA. Tetradecanoylphorbol Acetate 136-161 interleukin 4 Homo sapiens 15-19 7594459-3 1995 These activated T cells produced IL-2, IL-4, IL-5, and IFN-gamma upon stimulation with PMA and ionomycin. Tetradecanoylphorbol Acetate 87-90 interleukin 4 Homo sapiens 39-43 8555492-9 1996 In contrast to Con A and alpha CD3/alpha CD28 activation, phorbol myristate acetate plus A23187-induced IL-4 mRNA expression was insensitive to the inhibitory effect of db-cAMP and PGE2. Tetradecanoylphorbol Acetate 58-83 interleukin 4 Homo sapiens 104-108 8565269-5 1996 Data showed a significantly higher production of IL-4 (P = 0.05) and IL-10 (P < 0.005) as determined by ELISA in phytohaemagglutinin (PHA)/phorbol myristate acetate (PMA)-stimulated mononuclear cells of atopic donors compared with controls, although spontaneous IL-4 production without stimulation was never detected within either atopic or control groups. Tetradecanoylphorbol Acetate 142-167 interleukin 4 Homo sapiens 49-53 8565269-5 1996 Data showed a significantly higher production of IL-4 (P = 0.05) and IL-10 (P < 0.005) as determined by ELISA in phytohaemagglutinin (PHA)/phorbol myristate acetate (PMA)-stimulated mononuclear cells of atopic donors compared with controls, although spontaneous IL-4 production without stimulation was never detected within either atopic or control groups. Tetradecanoylphorbol Acetate 169-172 interleukin 4 Homo sapiens 49-53 8550072-5 1995 With adult purified T cells, high levels of IL-10 and IL-4 were measured following CD3 plus CD28 stimulation, and the amounts of both T-helper type-2 (Th2) cytokines decreased following the addition of phorbol myristate acetate (PMA), whereas the synthesis of the Th1 cytokines IL-2 and IFN-gamma was enhanced. Tetradecanoylphorbol Acetate 202-227 interleukin 4 Homo sapiens 54-58 7923875-2 1994 Upon stimulation with allergen or anti-CD3+ phorbol myristate acetate (PMA) IL-4 was released with or without IL-5, while no (or extremely low concentrations of) IL-2 and interferon-gamma (IFN-gamma) were detectable. Tetradecanoylphorbol Acetate 44-69 interleukin 4 Homo sapiens 76-80 7748537-4 1995 The release of interleukin-4 (IL-4) by PBMC stimulated with the isolated L. donovani antigen fractions was measured after treatment with phorbol-myristate-acetate and ionomycin. Tetradecanoylphorbol Acetate 137-162 interleukin 4 Homo sapiens 15-28 7748537-4 1995 The release of interleukin-4 (IL-4) by PBMC stimulated with the isolated L. donovani antigen fractions was measured after treatment with phorbol-myristate-acetate and ionomycin. Tetradecanoylphorbol Acetate 137-162 interleukin 4 Homo sapiens 30-34 7613170-6 1995 However, IL-4 secretion was only detected in cell cultures stimulated with PMA and ionomycin. Tetradecanoylphorbol Acetate 75-78 interleukin 4 Homo sapiens 9-13 7836748-5 1995 The most potent effects were observed with IL-4 and the phorbol ester, O-tetradecanoylphorbol-13-acetate (TPA), an activator of protein kinase C. IL-4 also partly inhibited TGF-beta 1 and forskolin-induced apoptosis. Tetradecanoylphorbol Acetate 106-109 interleukin 4 Homo sapiens 146-150 7923875-2 1994 Upon stimulation with allergen or anti-CD3+ phorbol myristate acetate (PMA) IL-4 was released with or without IL-5, while no (or extremely low concentrations of) IL-2 and interferon-gamma (IFN-gamma) were detectable. Tetradecanoylphorbol Acetate 71-74 interleukin 4 Homo sapiens 76-80 7913409-3 1994 On stimulation with phytohemagglutinin and phorbol 12-myristate 13-acetate, transplant-derived peripheral blood mononuclear cells demonstrate statistically significant depressed production of interleukin 3 (IL-3), IL-4, granulocyte-macrophage-colony-stimulating factor, and gamma-interferon as compared to normal controls, during the first 6 months following engraftment, which recover to normal levels 6 months or more posttransplant. Tetradecanoylphorbol Acetate 43-74 interleukin 4 Homo sapiens 214-218 8145051-10 1994 Activation with phorbol myristate acetate in combination with Ca-ionophore induced IL-4 secretion in both populations, but preferentially in the CDw60+ subset, whereas the vast majority of interferon gamma was produced by the CDw60-CD8+ cells. Tetradecanoylphorbol Acetate 16-41 interleukin 4 Homo sapiens 83-87 8018594-4 1994 In contrast, in cells stimulated with phorbol myristate acetate (PMA)/A23187, PGE2 enhanced the production of IL-4 and IL-5, and only partially inhibited the production of other cytokines. Tetradecanoylphorbol Acetate 38-63 interleukin 4 Homo sapiens 110-114 8018594-4 1994 In contrast, in cells stimulated with phorbol myristate acetate (PMA)/A23187, PGE2 enhanced the production of IL-4 and IL-5, and only partially inhibited the production of other cytokines. Tetradecanoylphorbol Acetate 65-68 interleukin 4 Homo sapiens 110-114 7909823-6 1994 Primed cells are enriched in CD45R0hi and CD31- cells, and upon stimulation with PMA+ ionomycin they release significant amounts of IL-2, IFN-gamma, IL-4, IL-5, and IL-10. Tetradecanoylphorbol Acetate 81-84 interleukin 4 Homo sapiens 149-153 8233727-11 1993 Considering the low proportion of lymphocytes, stimulation with phorbol myristate acetate in combination with ionomycin resulted in considerable production of the following lymphokines: IL-2, IL-3, IL-4, IL-10, interferon-gamma, tumor necrosis factor-alpha. Tetradecanoylphorbol Acetate 64-89 interleukin 4 Homo sapiens 198-202 7807282-6 1994 Whereas all activators induced significant IFN-gamma secretion, only ionomycin plus PMA stimulation induced large IL-4 secretion. Tetradecanoylphorbol Acetate 84-87 interleukin 4 Homo sapiens 114-118 8267044-6 1993 Interleukin-4 significantly enhanced the stimulatory actions of phorbol 12-myristate 13-acetate, ionomycin, and epidermal growth factor but not interleukin-1 beta on prostaglandin E2 production. Tetradecanoylphorbol Acetate 64-95 interleukin 4 Homo sapiens 0-13 8136266-2 1993 We found that interleukin 4 (IL-4), a T lymphocyte-derived cytokine known to regulate a number of monocyte functions, inhibited the production of TF by monocytes in response to endotoxin and phorbol myristate acetate (PMA) in vitro. Tetradecanoylphorbol Acetate 191-216 interleukin 4 Homo sapiens 14-27 8136266-2 1993 We found that interleukin 4 (IL-4), a T lymphocyte-derived cytokine known to regulate a number of monocyte functions, inhibited the production of TF by monocytes in response to endotoxin and phorbol myristate acetate (PMA) in vitro. Tetradecanoylphorbol Acetate 191-216 interleukin 4 Homo sapiens 29-33 8136266-2 1993 We found that interleukin 4 (IL-4), a T lymphocyte-derived cytokine known to regulate a number of monocyte functions, inhibited the production of TF by monocytes in response to endotoxin and phorbol myristate acetate (PMA) in vitro. Tetradecanoylphorbol Acetate 218-221 interleukin 4 Homo sapiens 14-27 8136266-2 1993 We found that interleukin 4 (IL-4), a T lymphocyte-derived cytokine known to regulate a number of monocyte functions, inhibited the production of TF by monocytes in response to endotoxin and phorbol myristate acetate (PMA) in vitro. Tetradecanoylphorbol Acetate 218-221 interleukin 4 Homo sapiens 29-33 8294141-4 1993 In addition PBMC incubated with LPGAP released interleukin-4 (IL-4) after pulsing with ionomycin and phorbol myristate acetate. Tetradecanoylphorbol Acetate 101-126 interleukin 4 Homo sapiens 47-60 8135784-1 1994 The P sequence of the human interleukin-4 (IL-4) gene, which was defined as a responsive element for phorbol 12-myristate 13-acetate and calcium ionophore (A23187) in Jurkat T cells, shares sequence similarity with the NF-kappa B and the NF-AT binding sites. Tetradecanoylphorbol Acetate 101-132 interleukin 4 Homo sapiens 28-41 8135784-1 1994 The P sequence of the human interleukin-4 (IL-4) gene, which was defined as a responsive element for phorbol 12-myristate 13-acetate and calcium ionophore (A23187) in Jurkat T cells, shares sequence similarity with the NF-kappa B and the NF-AT binding sites. Tetradecanoylphorbol Acetate 101-132 interleukin 4 Homo sapiens 43-47 7905497-4 1994 We report that prolonged exposure of immunologically naive and unstimulated human neonatal CD4 T cells to IL-4 or to IL-4 plus either IL-2 or IL-12 markedly affects their cytokine production on primary stimulation with PMA and ionomycin. Tetradecanoylphorbol Acetate 219-222 interleukin 4 Homo sapiens 106-110 7905497-4 1994 We report that prolonged exposure of immunologically naive and unstimulated human neonatal CD4 T cells to IL-4 or to IL-4 plus either IL-2 or IL-12 markedly affects their cytokine production on primary stimulation with PMA and ionomycin. Tetradecanoylphorbol Acetate 219-222 interleukin 4 Homo sapiens 117-121 7905497-7 1994 In response to primary stimulation with PMA and ionomycin, cells primed with IL-4 + IL-2 produce IL-4, IL-5, and IL-10 and the same levels of IL-2 and IFN-gamma as IL-4-primed cells. Tetradecanoylphorbol Acetate 40-43 interleukin 4 Homo sapiens 77-81 7905497-7 1994 In response to primary stimulation with PMA and ionomycin, cells primed with IL-4 + IL-2 produce IL-4, IL-5, and IL-10 and the same levels of IL-2 and IFN-gamma as IL-4-primed cells. Tetradecanoylphorbol Acetate 40-43 interleukin 4 Homo sapiens 97-101 7905497-7 1994 In response to primary stimulation with PMA and ionomycin, cells primed with IL-4 + IL-2 produce IL-4, IL-5, and IL-10 and the same levels of IL-2 and IFN-gamma as IL-4-primed cells. Tetradecanoylphorbol Acetate 40-43 interleukin 4 Homo sapiens 97-101 8457630-3 1993 Here we report that HUT78 cells can be induced to produce IL-4 either by CD3 stimulation under cross-linking conditions or by soluble CD3 mAbs in the presence of PMA. Tetradecanoylphorbol Acetate 162-165 interleukin 4 Homo sapiens 58-62 8388009-4 1993 Cultures stimulated with anti-CD3 or with phorbol myristate acetate plus ionophore significantly increased interleukin-4 production, and levels were consistently highest in cells from atopic subjects. Tetradecanoylphorbol Acetate 42-67 interleukin 4 Homo sapiens 107-120 8097058-4 1993 This paper shows that IL-4 alone was able to induce aggregation of B-CLL cells and to strongly enhance TPA+BSF-MP6-induced aggregation. Tetradecanoylphorbol Acetate 103-106 interleukin 4 Homo sapiens 22-26 8093439-3 1993 However, whereas T cell clones showed enhanced IL-4 production when phorbol 12-myristate 13-acetate (PMA) was used in addition to anti-CD2 and anti-CD28, IL-4 production by fresh T cells was inhibited by the presence of PMA. Tetradecanoylphorbol Acetate 68-99 interleukin 4 Homo sapiens 47-51 8417800-12 1993 Furthermore, it appeared that the induction of IL-4 mRNA was dependent on protein synthesis because cycloheximide (CHX) blocked the Con A- and Con A+PMA-induced expression of IL-4 mRNA. Tetradecanoylphorbol Acetate 149-152 interleukin 4 Homo sapiens 47-51 8417800-12 1993 Furthermore, it appeared that the induction of IL-4 mRNA was dependent on protein synthesis because cycloheximide (CHX) blocked the Con A- and Con A+PMA-induced expression of IL-4 mRNA. Tetradecanoylphorbol Acetate 149-152 interleukin 4 Homo sapiens 175-179 8093439-3 1993 However, whereas T cell clones showed enhanced IL-4 production when phorbol 12-myristate 13-acetate (PMA) was used in addition to anti-CD2 and anti-CD28, IL-4 production by fresh T cells was inhibited by the presence of PMA. Tetradecanoylphorbol Acetate 68-99 interleukin 4 Homo sapiens 154-158 8419187-4 1993 After stimulation with phorbol 12-myristate 13-acetate (PMA) and calcium ionophore A23187, they produced higher levels of IL-4 (306 vs. 55 +/- 4 pg/ml) and IL-5 (2900 vs. 213 +/- 72 pg/ml) and lower levels of IL-2 (17 vs. 63 +/- 17 IU/ml) and interferon-gamma (IFN-gamma) (16 vs. 299 +/- 70 IU/ml) than controls CD4+ CD45R0+ cells. Tetradecanoylphorbol Acetate 23-54 interleukin 4 Homo sapiens 122-126 8419187-4 1993 After stimulation with phorbol 12-myristate 13-acetate (PMA) and calcium ionophore A23187, they produced higher levels of IL-4 (306 vs. 55 +/- 4 pg/ml) and IL-5 (2900 vs. 213 +/- 72 pg/ml) and lower levels of IL-2 (17 vs. 63 +/- 17 IU/ml) and interferon-gamma (IFN-gamma) (16 vs. 299 +/- 70 IU/ml) than controls CD4+ CD45R0+ cells. Tetradecanoylphorbol Acetate 56-59 interleukin 4 Homo sapiens 122-126 8093439-3 1993 However, whereas T cell clones showed enhanced IL-4 production when phorbol 12-myristate 13-acetate (PMA) was used in addition to anti-CD2 and anti-CD28, IL-4 production by fresh T cells was inhibited by the presence of PMA. Tetradecanoylphorbol Acetate 101-104 interleukin 4 Homo sapiens 47-51 1377987-5 1992 In addition, the pyruvate kinase C (PKC) pathway does not seem to participate in the process either because in our system activation of PKC by 4 beta-phorbol 12 beta-myristate 13 alpha-acetate (PMA) is insufficient by itself to induce HLA-DR. We found, however, that a second messenger pathway can be mediated by a G protein system since IL-4 concomitantly induces class II and p21ras expression which can be successfully blocked by a highly specific anti-p21ras monoclonal antibody. Tetradecanoylphorbol Acetate 194-197 interleukin 4 Homo sapiens 338-342 1358967-8 1992 In functional assays, IL-4 production could only be induced in CD45RA-CD27- CD4 clones by stimulation with PMA and ionomycin. Tetradecanoylphorbol Acetate 107-110 interleukin 4 Homo sapiens 22-26 1498085-5 1992 Phorbol myristate acetate enhanced its expression in DND39 cells as well as in the purified B cells in combination with IL-4. Tetradecanoylphorbol Acetate 0-25 interleukin 4 Homo sapiens 120-124 1398515-5 1992 Supernatants of TPA-stimulated Co cells contained the cytokines IL2, IL3, IL4 and IL8, whereas these cytokines were not detected in the supernatants of untreated cells. Tetradecanoylphorbol Acetate 16-19 interleukin 4 Homo sapiens 74-77 1512301-6 1992 After stimulation with calcium ionophore A23187 and phorbol myristic acetate (PMA), the production of IL-4 was markedly increased in both patients and normals. Tetradecanoylphorbol Acetate 78-81 interleukin 4 Homo sapiens 102-106 1373168-7 1992 IL-4 most likely utilizes a protein kinase C-independent signal transduction pathway to modify CD20 molecule inasmuch as staurosporine, an inhibitor of protein kinase C, antagonizes phorbol esters (PMA) but not IL-4-induced CD20 down-regulation. Tetradecanoylphorbol Acetate 198-201 interleukin 4 Homo sapiens 0-4 1734879-1 1992 Preexposure of human monocytes to recombinant human interleukin-4 (IL-4) suppressed the respiratory burst response to a number of different stimuli, including phorbol myristate acetate, zymosan, platelet-activating factor and the chemotactic peptide, f-met-leu-phe. Tetradecanoylphorbol Acetate 159-184 interleukin 4 Homo sapiens 52-65 1370514-8 1992 Stimulation with Con A also induced very low or no measurable levels of IL-2 and IFN-gamma, whereas activation with TPA and the calcium ionophore A23187 resulted in the production of high levels of IL-4, IL-5, IL-2, and IFN-gamma. Tetradecanoylphorbol Acetate 116-119 interleukin 4 Homo sapiens 198-202 1734879-1 1992 Preexposure of human monocytes to recombinant human interleukin-4 (IL-4) suppressed the respiratory burst response to a number of different stimuli, including phorbol myristate acetate, zymosan, platelet-activating factor and the chemotactic peptide, f-met-leu-phe. Tetradecanoylphorbol Acetate 159-184 interleukin 4 Homo sapiens 67-71 1345920-8 1992 Finally, IL-4 production could only be induced by stimulation with PMA and ionomycin in either resting or activated CD31- CD4 cells. Tetradecanoylphorbol Acetate 67-70 interleukin 4 Homo sapiens 9-13 1371491-8 1992 When the cells were stimulated by phorbol ester (phorbol 12-myristate 13-acetate, PMA) plus calcium ionophore (ionomycin), FK506 and CsA inhibited, in a dose-dependent manner, the production of IL-2, IL-4, IL-5, IFN-gamma and TNF-alpha. Tetradecanoylphorbol Acetate 49-80 interleukin 4 Homo sapiens 200-204 1371491-8 1992 When the cells were stimulated by phorbol ester (phorbol 12-myristate 13-acetate, PMA) plus calcium ionophore (ionomycin), FK506 and CsA inhibited, in a dose-dependent manner, the production of IL-2, IL-4, IL-5, IFN-gamma and TNF-alpha. Tetradecanoylphorbol Acetate 82-85 interleukin 4 Homo sapiens 200-204 1761792-1 1991 T cell growth factors such as interleukin-2 (IL-2) and interleukin-4 (IL-4) act as potent comitogenic factors for purified human T cells in the presence of phorbol myristate acetate (PMA). Tetradecanoylphorbol Acetate 156-181 interleukin 4 Homo sapiens 55-68 1761792-1 1991 T cell growth factors such as interleukin-2 (IL-2) and interleukin-4 (IL-4) act as potent comitogenic factors for purified human T cells in the presence of phorbol myristate acetate (PMA). Tetradecanoylphorbol Acetate 156-181 interleukin 4 Homo sapiens 70-74 1761792-1 1991 T cell growth factors such as interleukin-2 (IL-2) and interleukin-4 (IL-4) act as potent comitogenic factors for purified human T cells in the presence of phorbol myristate acetate (PMA). Tetradecanoylphorbol Acetate 183-186 interleukin 4 Homo sapiens 55-68 1761792-1 1991 T cell growth factors such as interleukin-2 (IL-2) and interleukin-4 (IL-4) act as potent comitogenic factors for purified human T cells in the presence of phorbol myristate acetate (PMA). Tetradecanoylphorbol Acetate 183-186 interleukin 4 Homo sapiens 70-74 2166625-8 1990 Additive effects of IL-4 and IFN were on the other hand seen on HLA-DR and HLA-DQ expression as well as on O2- production in response to stimulation with phorbol ester (PMA). Tetradecanoylphorbol Acetate 169-172 interleukin 4 Homo sapiens 20-32 1718026-5 1991 By stimulation with phytohaemagglutinin (PHA) plus phorbol myristate acetate (PMA), three of the seven helper-type clones produced interleukin 2 (IL-2) in addition to IL-4. Tetradecanoylphorbol Acetate 78-81 interleukin 4 Homo sapiens 167-171 1911538-3 1991 When the human IgM-producing cell line SSK41 is stimulated with interleukin 4 (IL-4) in the presence of phorbol myristate acetate (PMA) or Staphylococcus aureus Cowan I, expression of germline C gamma 3 transcripts was specifically augmented within 4 h. Upstream DNA fragments flanking the I gamma 3 exon were fused with a reporter gene and tested for IL-4-induced promoter/enhancer activity by transfection of SSK41 cells. Tetradecanoylphorbol Acetate 131-134 interleukin 4 Homo sapiens 64-77 2295806-1 1990 12-O-Tetradecanoylphorbol-13-acetate (TPA), a tumor-promoting phorbol ester, induced the proliferation of connective tissue-type mast cells (CTMC) synergistically with IL-3 in a methylcellulose culture, as well as with IL-4. Tetradecanoylphorbol Acetate 0-36 interleukin 4 Homo sapiens 219-223 2295806-1 1990 12-O-Tetradecanoylphorbol-13-acetate (TPA), a tumor-promoting phorbol ester, induced the proliferation of connective tissue-type mast cells (CTMC) synergistically with IL-3 in a methylcellulose culture, as well as with IL-4. Tetradecanoylphorbol Acetate 38-41 interleukin 4 Homo sapiens 219-223 34592416-5 2021 The PMA-treated S1P2knockout THP-1 Mphis showed decreases in IL-4/IL-13-induced phosphorylation of Janus-activated kinase (JAK) 1, JAK2, and STAT6 as well as mRNA expression of the M2 marker ARG1 compared with wild-type THP-1 Mphis. Tetradecanoylphorbol Acetate 4-7 interleukin 4 Homo sapiens 61-65 34592416-6 2021 Pretreatment of PMA-treated THP-1 Mphis with the S1P2 antagonist JTE-013, the Rho inhibitor Rhosin or the Rho kinase inhibitor Y27632 inhibited the IL-4/IL-13-induced increase in STAT6 phosphorylation. Tetradecanoylphorbol Acetate 16-19 interleukin 4 Homo sapiens 148-152