PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 18067864-0 2007 Phorbol myristate acetate-induced Egr-1 expression is suppressed by phospholipase D isozymes in human glioma cells. Tetradecanoylphorbol Acetate 0-25 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 68-83 20158570-5 2011 Using 1-butanol, a PLD substrate, to block phosphatidic acid (PA) generation, the PMA-stimulated neutrophil respiratory burst was also partially inhibited, further indicating that PLD activation, possibly its hydrolytic product PA and diacylglycerol (DAG), is involved in PMA-stimulated respiratory burst. Tetradecanoylphorbol Acetate 82-85 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 180-183 20158570-5 2011 Using 1-butanol, a PLD substrate, to block phosphatidic acid (PA) generation, the PMA-stimulated neutrophil respiratory burst was also partially inhibited, further indicating that PLD activation, possibly its hydrolytic product PA and diacylglycerol (DAG), is involved in PMA-stimulated respiratory burst. Tetradecanoylphorbol Acetate 272-275 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 180-183 20158570-7 2011 To further study whether PLD contributes to the PMA stimulated respiratory burst through itself or its hydrolytic product, 1,2-dioctanoyl-sn-glycerol, an analogue of DAG , was used to prime cells at low concentration, and it reversed the inhibition of PMA-stimulated respiratory burst by U73122. Tetradecanoylphorbol Acetate 48-51 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 25-28 20158570-8 2011 The results indicate that U73122 may act as an inhibitor of PLD, and PLD activation is required in PMA-stimulated respiratory burst. Tetradecanoylphorbol Acetate 99-102 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 69-72 20158570-2 2011 However, the PLD activity was also increased by 10-fold in human neutrophils stimulated with 100 nM PMA. Tetradecanoylphorbol Acetate 100-103 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 13-16 20158570-5 2011 Using 1-butanol, a PLD substrate, to block phosphatidic acid (PA) generation, the PMA-stimulated neutrophil respiratory burst was also partially inhibited, further indicating that PLD activation, possibly its hydrolytic product PA and diacylglycerol (DAG), is involved in PMA-stimulated respiratory burst. Tetradecanoylphorbol Acetate 82-85 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 19-22 18067864-6 2007 Taken together, these results suggest that elevated expression and activity of PLD attenuate PMA-induced Egr-1 expression via PI3K pathway. Tetradecanoylphorbol Acetate 93-96 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 79-82 18067864-2 2007 Here, we found that overexpression of phospholipase D (PLD) isozymes decreased tumor promoter phorbol myristate acetate (PMA)-induced Egr-1 expression and transactivation in glioma cells. Tetradecanoylphorbol Acetate 94-119 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 38-53 18067864-2 2007 Here, we found that overexpression of phospholipase D (PLD) isozymes decreased tumor promoter phorbol myristate acetate (PMA)-induced Egr-1 expression and transactivation in glioma cells. Tetradecanoylphorbol Acetate 94-119 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 55-58 18067864-2 2007 Here, we found that overexpression of phospholipase D (PLD) isozymes decreased tumor promoter phorbol myristate acetate (PMA)-induced Egr-1 expression and transactivation in glioma cells. Tetradecanoylphorbol Acetate 121-124 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 38-53 18067864-2 2007 Here, we found that overexpression of phospholipase D (PLD) isozymes decreased tumor promoter phorbol myristate acetate (PMA)-induced Egr-1 expression and transactivation in glioma cells. Tetradecanoylphorbol Acetate 121-124 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 55-58 16870152-6 2006 The phorbol myristate acetate stimulated the PLD activities of pCDNA3.1-PLD-treated neutrophils but did not stimulate the activities of untreated or pCDNA3.1-treated neutrophils. Tetradecanoylphorbol Acetate 4-29 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 45-48 17130901-1 2007 Phospholipase D (PLD) is a ubiquitous enzyme that can be activated by extracellular adenosine 5"-triphosphate (ATP) or phorbol 12-myristate 13-acetate (PMA) in B-lymphocytes from subjects with chronic lymphocytic leukaemia (CLL). Tetradecanoylphorbol Acetate 119-150 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 0-15 17130901-1 2007 Phospholipase D (PLD) is a ubiquitous enzyme that can be activated by extracellular adenosine 5"-triphosphate (ATP) or phorbol 12-myristate 13-acetate (PMA) in B-lymphocytes from subjects with chronic lymphocytic leukaemia (CLL). Tetradecanoylphorbol Acetate 119-150 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 17-20 17130901-1 2007 Phospholipase D (PLD) is a ubiquitous enzyme that can be activated by extracellular adenosine 5"-triphosphate (ATP) or phorbol 12-myristate 13-acetate (PMA) in B-lymphocytes from subjects with chronic lymphocytic leukaemia (CLL). Tetradecanoylphorbol Acetate 152-155 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 0-15 17130901-1 2007 Phospholipase D (PLD) is a ubiquitous enzyme that can be activated by extracellular adenosine 5"-triphosphate (ATP) or phorbol 12-myristate 13-acetate (PMA) in B-lymphocytes from subjects with chronic lymphocytic leukaemia (CLL). Tetradecanoylphorbol Acetate 152-155 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 17-20 16870152-6 2006 The phorbol myristate acetate stimulated the PLD activities of pCDNA3.1-PLD-treated neutrophils but did not stimulate the activities of untreated or pCDNA3.1-treated neutrophils. Tetradecanoylphorbol Acetate 4-29 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 72-75 15591778-2 2004 Direct activation of protein kinase C (PKC) by phorbol-12-myristate-13-acetate (PMA) also stimulates PLD in this tissue. Tetradecanoylphorbol Acetate 47-78 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 101-104 16520553-1 2006 Elevated expression of protein casein kinase II (CKII) stimulated basal phospholipase D (PLD) activity as well as PMA-induced PLD activation in human U87 astroglioma cells. Tetradecanoylphorbol Acetate 114-117 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 126-129 16188211-12 2005 Two isoforms of PLD, oleate-dependent and TPA-dependent, are also present in LA-N-1 cell homogenates. Tetradecanoylphorbol Acetate 42-45 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 16-19 15389577-10 2005 We conclude that PMA-induced membrane ruffling is caused via ARF6-Rac1 and ARF1 pathways operating in parallel and that PLD may be inhibitory. Tetradecanoylphorbol Acetate 17-20 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 120-123 15591778-2 2004 Direct activation of protein kinase C (PKC) by phorbol-12-myristate-13-acetate (PMA) also stimulates PLD in this tissue. Tetradecanoylphorbol Acetate 80-83 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 101-104 15557817-6 2004 Phorbol 12-myristate 13-acetate (PMA), which is known to activate PKC, stimulated PLD activity significantly more in the core protein-transformed cells, in comparison with that of the control cells. Tetradecanoylphorbol Acetate 33-36 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 82-85 15557817-6 2004 Phorbol 12-myristate 13-acetate (PMA), which is known to activate PKC, stimulated PLD activity significantly more in the core protein-transformed cells, in comparison with that of the control cells. Tetradecanoylphorbol Acetate 0-31 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 82-85 15557817-7 2004 PLD activity assay using PKC isozyme-specific inhibitor and PKC translocation experiment showed that PKC-delta was mainly involved in the PMA- induced PLD activation in the core-transformed cells. Tetradecanoylphorbol Acetate 138-141 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 0-3 15557817-7 2004 PLD activity assay using PKC isozyme-specific inhibitor and PKC translocation experiment showed that PKC-delta was mainly involved in the PMA- induced PLD activation in the core-transformed cells. Tetradecanoylphorbol Acetate 138-141 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 151-154 15557821-4 2004 Down-regulation of PKC-alpha with phorbol myristate acetate pretreatment for 24 h abolished Der f 2-induced PLD activation. Tetradecanoylphorbol Acetate 34-59 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 108-111 12943994-1 2003 In this report we demonstrate that in human adrenocortical carcinoma NCI H295R cells, a model for adrenal glomerulosa cells, PLD was activated both by AngII and protein kinase C (PKC)-activating phorbol 12-myristate 13-acetate (PMA). Tetradecanoylphorbol Acetate 195-226 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 125-128 15020229-3 2004 Retinoic acid (RA)-induced differentiation increased PLD1 and PLD2 mRNA levels and PLD activity that was responsive to phorbol myristate acetate. Tetradecanoylphorbol Acetate 119-144 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 53-56 15030177-8 2004 Using [3H]choline chloride to prelabel MCs and measuring [3H]choline-containing metabolite release as PLD activity, PMA stimulated a significant increase of PLD activity under high glucose condition. Tetradecanoylphorbol Acetate 116-119 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 102-105 15030177-8 2004 Using [3H]choline chloride to prelabel MCs and measuring [3H]choline-containing metabolite release as PLD activity, PMA stimulated a significant increase of PLD activity under high glucose condition. Tetradecanoylphorbol Acetate 116-119 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 157-160 14984736-4 2004 When either isoform of PLD was stably expressed in HEK-293 cells, we observed an increased PLD activity in a cell-free system and a 12-O-tetradecanoyl-13-phorbol acetate (TPA)-stimulated increase in PLD activity in intact cells. Tetradecanoylphorbol Acetate 171-174 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 23-26 14984736-5 2004 This system was then used to elucidate the effects of PLD activity on TPA-stimulated signaling pathways. Tetradecanoylphorbol Acetate 70-73 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 54-57 15081395-5 2004 On the contrary, phorbol ester (12-O-tetradecanoylphorbol-13-acetate, TPA)-mediated PLD activity was enhanced by GTPgammaS and was only partially decreased by methyl-beta-cyclodextrin. Tetradecanoylphorbol Acetate 32-68 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 84-87 12706826-1 2003 Earlier studies showed that treatment of LA-N-1 cells with TPA, a tumoral promoter, leads to the stimulation of a G protein-regulated phospholipase D (PLD) in the nuclei. Tetradecanoylphorbol Acetate 59-62 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 134-149 12706826-1 2003 Earlier studies showed that treatment of LA-N-1 cells with TPA, a tumoral promoter, leads to the stimulation of a G protein-regulated phospholipase D (PLD) in the nuclei. Tetradecanoylphorbol Acetate 59-62 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 151-154 12074591-2 2002 In G361 cell line, which lacks PKCalpha, 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced PLD activation was potentiated by introducing PKCalpha by the adenovirus vector. Tetradecanoylphorbol Acetate 41-77 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 92-95 12606037-4 2003 Overexpression of arfaptin 1, a 39 kDa ARF-binding protein that inhibits in vitro activation of cholera toxin ADP-ribosyltransferase and phospholipase D (PLD) by ARF, inhibited PMA-stimulated MMP-9 and PLD activation. Tetradecanoylphorbol Acetate 177-180 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 154-157 12074591-2 2002 In G361 cell line, which lacks PKCalpha, 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced PLD activation was potentiated by introducing PKCalpha by the adenovirus vector. Tetradecanoylphorbol Acetate 79-82 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 92-95 12074591-3 2002 The kinase-negative PKCalpha elevated TPA-induced PLD activity less significantly than the wild type. Tetradecanoylphorbol Acetate 38-41 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 50-53 12074591-5 2002 Expression of PKCbetaII and the kinase-negative PKCbetaII enhanced TPA-stimulated PLD activity moderately in MeWo cell line, in which PKCbetaII is absent. Tetradecanoylphorbol Acetate 67-70 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 82-85 11944907-4 2002 Genistein inhibited PLD activity with an IC(50) value of 12.2 microM in fMLP- and 107 microM in phorbol myristate acetate (PMA)-stimulated cells. Tetradecanoylphorbol Acetate 96-121 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 20-23 11821392-5 2002 Treatment with pervanadate or phorbol myristate acetate inhibits PLD more in HEK 293 cells overexpressing alpha-synuclein than in control cells. Tetradecanoylphorbol Acetate 30-55 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 65-68 11944907-4 2002 Genistein inhibited PLD activity with an IC(50) value of 12.2 microM in fMLP- and 107 microM in phorbol myristate acetate (PMA)-stimulated cells. Tetradecanoylphorbol Acetate 123-126 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 20-23 11419696-3 2001 The PLD activation induced by BK was blocked by pretreatment of A-431 cells with staurosporine, or by prolonged treatment with phorbol-12-myristate-13-acetate (PMA). Tetradecanoylphorbol Acetate 127-158 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 4-7 11734063-4 2001 RESULTS: PLD2, but not PLD1, mRNA and protein were detected in these cells and endogenous PLD activity and ACh synthesis were stimulated by phorbol 12-myristate 13-acetate (PMA). Tetradecanoylphorbol Acetate 173-176 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 9-12 11734063-9 2001 Overexpression of human PLD1 by transient transfection increased PLD activity by 4.6-fold and ACh synthesis by 2.3-fold in the presence of PMA as compared to controls. Tetradecanoylphorbol Acetate 139-142 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 24-27 11380624-2 2001 In this study, we investigated the roles of Syk and other PTKs for the phorbol esters, 12-O-tetradecanoylphorbol 13-acetate (TPA)-induced PLD activation in K562 and DT40 cells. Tetradecanoylphorbol Acetate 87-123 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 138-141 11380624-2 2001 In this study, we investigated the roles of Syk and other PTKs for the phorbol esters, 12-O-tetradecanoylphorbol 13-acetate (TPA)-induced PLD activation in K562 and DT40 cells. Tetradecanoylphorbol Acetate 125-128 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 138-141 11380624-3 2001 RESULTS: TPA-induced PLD activation was remarkably reduced in both Syk dominant negative mutant K562 cells and Syk deficient DT40 B cells. Tetradecanoylphorbol Acetate 9-12 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 21-24 11380624-5 2001 Similarly, TPA-induced PLD activation was reduced in Btk deficient cells, but unaffected in Lyn deficient cells. Tetradecanoylphorbol Acetate 11-14 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 23-26 11380624-6 2001 Finally, in cells deficient in the PLC-gamma2, one of the phosphorylated substrates regulated by Syk and Btk, TPA-induced PLD activation, as well as phosphatidylinositol 4,5-bisphosphate (PIP2) hydrolysis was remarkably reduced. Tetradecanoylphorbol Acetate 110-113 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 122-125 11380624-7 2001 CONCLUSIONS: We demonstrated that the Syk, Btk and PLC-gamma2 pathways are required for TPA-induced PLD activation in DT40 cells. Tetradecanoylphorbol Acetate 88-91 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 100-103 11336804-8 2001 PLD activity was furthermore increased by 8Br-cAMP and following acute (30 min) stimulation of protein kinase C (PKC) with a phorbol ester (PMA). Tetradecanoylphorbol Acetate 140-143 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 0-3 11419696-3 2001 The PLD activation induced by BK was blocked by pretreatment of A-431 cells with staurosporine, or by prolonged treatment with phorbol-12-myristate-13-acetate (PMA). Tetradecanoylphorbol Acetate 160-163 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 4-7 11018470-2 2000 In SK-N-MC human neuroblastoma cells, phorbol ester (TPA) activation of PLD was enhanced by overexpressing myristoylated alanine-rich C kinase substrate (MARCKS). Tetradecanoylphorbol Acetate 53-56 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 72-75 11063592-3 2000 We now report that stimulation of PMN with proinflammatory agonist N-formyl peptides (FMLP), calcium ionophore A(23187), or phorbol mirystate acetate (PMA) is followed by marked downregulation of LXA(4) binding (B(max) decrease of approximately 45%) and decreased activation of phospholipases A(2) (PLA(2)) and D (PLD). Tetradecanoylphorbol Acetate 151-154 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 314-317 11018470-5 2000 TPA-stimulated PLD activity was higher in both intact and digitonin-permeabilized M22 cells than in vector controls. Tetradecanoylphorbol Acetate 0-3 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 15-18 11018470-9 2000 Disruption of cholesterol-rich DIFs with digitonin, cyclodextrin or filipin potentiated activation of PLD by TPA. Tetradecanoylphorbol Acetate 109-112 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 102-105 10926117-2 2000 When the cells were pretreated for 24 h with phorbol myristate acetate to down-regulate protein kinase C (PKC), PLD stimulation by Der p I was largely abolished. Tetradecanoylphorbol Acetate 45-70 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 112-115 10614786-2 1999 Three agonists known to stimulate PLD activity, fMet-Leu-Phe (fMLP), phorbol 12-myristate 13-acetate (PMA) and V4+-OOH, induced a differential translocation of ADP-ribosylation factor (ARF), RhoA, and protein kinase Calpha (PKCalpha), all cofactors for PLD activation. Tetradecanoylphorbol Acetate 69-100 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 34-37 10567919-8 2000 In PC12K cells, which express only PLD2, treatment with nerve growth factor causes neurite outgrowth and increases expression of PLD2 mRNA and protein within 6-12 h. A corresponding increase is observed in membrane PLD activity and in phorbol-12-myristate-13-acetate (PMA)-stimulated PLD activity in intact cells. Tetradecanoylphorbol Acetate 268-271 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 129-132 10798219-5 2000 Ceramide also inhibits phorbol myristate acetate-stimulated PLD. Tetradecanoylphorbol Acetate 23-48 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 60-63 10798219-7 2000 There was an increase in PLD activity in the presence of phorbol myristate acetate in cells supplemented with 16 microM SIT compared with those supplemented with cholesterol after five days of treatment. Tetradecanoylphorbol Acetate 57-82 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 25-28 10614786-2 1999 Three agonists known to stimulate PLD activity, fMet-Leu-Phe (fMLP), phorbol 12-myristate 13-acetate (PMA) and V4+-OOH, induced a differential translocation of ADP-ribosylation factor (ARF), RhoA, and protein kinase Calpha (PKCalpha), all cofactors for PLD activation. Tetradecanoylphorbol Acetate 69-100 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 253-256 10614786-2 1999 Three agonists known to stimulate PLD activity, fMet-Leu-Phe (fMLP), phorbol 12-myristate 13-acetate (PMA) and V4+-OOH, induced a differential translocation of ADP-ribosylation factor (ARF), RhoA, and protein kinase Calpha (PKCalpha), all cofactors for PLD activation. Tetradecanoylphorbol Acetate 102-105 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 34-37 10373471-3 1999 We investigated in intact (human adenocarcinoma A549 cells) the role of RhoA and ARF in activation of PLD by phorbol 12-myristate 13-acetate, bradykinin, and/or sphingosine 1-phosphate. Tetradecanoylphorbol Acetate 109-140 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 102-105 10373471-5 1999 Expression of C3 toxin or Rho19N increased basal and decreased phorbol 12-myristate 13-acetate-stimulated PLD activity. Tetradecanoylphorbol Acetate 63-94 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 106-109 10198344-0 1999 1,25-dihydroxyvitamin D3 and TPA activate phospholipase D in Caco-2 cells: role of PKC-alpha. Tetradecanoylphorbol Acetate 29-32 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 42-57 10318815-3 1999 We show that guanosine 5"-O-(3-thiotriphosphate)-stimulated phospholipase D (PLD) activity is inhibited in a time- and dose-dependent manner after an overnight treatment with LT. A similar dose response to the toxin was found when PLD activity was stimulated by phorbol 12-myristate 13-acetate via the protein kinase C pathway. Tetradecanoylphorbol Acetate 262-293 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 60-75 10318815-3 1999 We show that guanosine 5"-O-(3-thiotriphosphate)-stimulated phospholipase D (PLD) activity is inhibited in a time- and dose-dependent manner after an overnight treatment with LT. A similar dose response to the toxin was found when PLD activity was stimulated by phorbol 12-myristate 13-acetate via the protein kinase C pathway. Tetradecanoylphorbol Acetate 262-293 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 77-80 10198345-7 1999 We therefore determined whether these signal transduction elements were involved in PLD stimulation by 1,25(OH)2D3 or TPA. Tetradecanoylphorbol Acetate 118-121 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 84-87 10198344-1 1999 1,25-Dihydroxyvitamin D3 [1,25(OH)2D3] and 12-O-tetradecanoylphorbol 13-acetate (TPA) both activated phospholipase D (PLD) in Caco-2 cells. Tetradecanoylphorbol Acetate 43-79 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 101-116 10198345-14 1999 These findings, taken together with those of the accompanying paper, indicate that although 1,25(OH)2D3 and TPA each activate PLD in Caco-2 cells in part via PKC-alpha, their stimulation of PLD differs in a number of important aspects, including the requirement for pp60(c-src) and RhoA in the activation of PLD by 1,25(OH)2D3, but not TPA. Tetradecanoylphorbol Acetate 108-111 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 126-129 10198345-14 1999 These findings, taken together with those of the accompanying paper, indicate that although 1,25(OH)2D3 and TPA each activate PLD in Caco-2 cells in part via PKC-alpha, their stimulation of PLD differs in a number of important aspects, including the requirement for pp60(c-src) and RhoA in the activation of PLD by 1,25(OH)2D3, but not TPA. Tetradecanoylphorbol Acetate 108-111 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 190-193 10198345-14 1999 These findings, taken together with those of the accompanying paper, indicate that although 1,25(OH)2D3 and TPA each activate PLD in Caco-2 cells in part via PKC-alpha, their stimulation of PLD differs in a number of important aspects, including the requirement for pp60(c-src) and RhoA in the activation of PLD by 1,25(OH)2D3, but not TPA. Tetradecanoylphorbol Acetate 108-111 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 190-193 10198345-15 1999 Moreover, the requirement for different signal transduction elements by 1,25(OH)2D3 and TPA to induce the stimulation of PLD may potentially underlie differences in the physiological effects of these agents in Caco-2 cells. Tetradecanoylphorbol Acetate 88-91 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 121-124 10198344-1 1999 1,25-Dihydroxyvitamin D3 [1,25(OH)2D3] and 12-O-tetradecanoylphorbol 13-acetate (TPA) both activated phospholipase D (PLD) in Caco-2 cells. Tetradecanoylphorbol Acetate 43-79 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 118-121 10198344-1 1999 1,25-Dihydroxyvitamin D3 [1,25(OH)2D3] and 12-O-tetradecanoylphorbol 13-acetate (TPA) both activated phospholipase D (PLD) in Caco-2 cells. Tetradecanoylphorbol Acetate 81-84 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 101-116 10198344-1 1999 1,25-Dihydroxyvitamin D3 [1,25(OH)2D3] and 12-O-tetradecanoylphorbol 13-acetate (TPA) both activated phospholipase D (PLD) in Caco-2 cells. Tetradecanoylphorbol Acetate 81-84 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 118-121 10198344-4 1999 Chronic treatment with TPA (1 microM, 24 h) significantly reduced the expression of PKC-alpha, -betaI, and -delta and markedly reduced the ability of 1,25(OH)2D3 or TPA to acutely stimulate PLD. Tetradecanoylphorbol Acetate 23-26 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 190-193 10198344-4 1999 Chronic treatment with TPA (1 microM, 24 h) significantly reduced the expression of PKC-alpha, -betaI, and -delta and markedly reduced the ability of 1,25(OH)2D3 or TPA to acutely stimulate PLD. Tetradecanoylphorbol Acetate 165-168 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 190-193 10198344-5 1999 Removal of Ca2+ from the medium, as well as preincubation of cells with Go-6976, an inhibitor of Ca2+-dependent PKC isoforms, significantly reduced the stimulation of PLD by 1,25(OH)2D3 or TPA. Tetradecanoylphorbol Acetate 189-192 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 167-170 10198344-7 1999 In addition, the activation of PLD by 1,25(OH)2D3 or TPA was markedly reduced or accentuated in stably transfected cells with inhibited or amplified PKC-alpha expression, respectively. Tetradecanoylphorbol Acetate 53-56 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 31-34 10198344-8 1999 Taken together, these observations indicate that PKC-alpha is intimately involved in the stimulation of PLD in Caco-2 cells by 1,25(OH)2D3 or TPA. Tetradecanoylphorbol Acetate 142-145 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 104-107 10198345-5 1999 39): G993-G1004, 1999], activation of protein kinase C-alpha (PKC-alpha) was shown to be involved in the stimulation of phospholipase D (PLD) by 1,25-dihydroxyvitamin D3 [1, 25(OH)2D3] and 12-O-tetradecanoylphorbol 13-acetate (TPA) in Caco-2 cells. Tetradecanoylphorbol Acetate 189-225 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 137-140 10198345-5 1999 39): G993-G1004, 1999], activation of protein kinase C-alpha (PKC-alpha) was shown to be involved in the stimulation of phospholipase D (PLD) by 1,25-dihydroxyvitamin D3 [1, 25(OH)2D3] and 12-O-tetradecanoylphorbol 13-acetate (TPA) in Caco-2 cells. Tetradecanoylphorbol Acetate 227-230 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 137-140 10087440-6 1999 Activation of protein kinase C (PKC) by phorbol-12-myristate-13-acetate (PMA) increased PLD activity that was effectively blocked by the PKC inhibitors calphostin C (10(-8) to 10(-6) M) and GFX (10(-8) to 10(-6) M). Tetradecanoylphorbol Acetate 40-71 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 88-91 10049506-1 1999 In fibroblasts, the protein kinase C (PKC) activator phorbol 12-myristate 13-acetate (PMA) stimulates phospholipase D (PLD)-mediated hydrolysis of both phosphatidylcholine (PtdCho) and phosphatidylethanolamine (PtdEtn) by PKC-alpha-mediated nonphosphorylating and phosphorylating mechanisms. Tetradecanoylphorbol Acetate 53-84 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 102-117 10049506-1 1999 In fibroblasts, the protein kinase C (PKC) activator phorbol 12-myristate 13-acetate (PMA) stimulates phospholipase D (PLD)-mediated hydrolysis of both phosphatidylcholine (PtdCho) and phosphatidylethanolamine (PtdEtn) by PKC-alpha-mediated nonphosphorylating and phosphorylating mechanisms. Tetradecanoylphorbol Acetate 53-84 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 119-122 10049506-1 1999 In fibroblasts, the protein kinase C (PKC) activator phorbol 12-myristate 13-acetate (PMA) stimulates phospholipase D (PLD)-mediated hydrolysis of both phosphatidylcholine (PtdCho) and phosphatidylethanolamine (PtdEtn) by PKC-alpha-mediated nonphosphorylating and phosphorylating mechanisms. Tetradecanoylphorbol Acetate 86-89 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 102-117 10049506-1 1999 In fibroblasts, the protein kinase C (PKC) activator phorbol 12-myristate 13-acetate (PMA) stimulates phospholipase D (PLD)-mediated hydrolysis of both phosphatidylcholine (PtdCho) and phosphatidylethanolamine (PtdEtn) by PKC-alpha-mediated nonphosphorylating and phosphorylating mechanisms. Tetradecanoylphorbol Acetate 86-89 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 119-122 10527453-7 1999 However, despite the ability of phorbol myristate acetate (PMA) to stimulate phospholipase D (PLD) and synthesis of phosphatidylethanol (PEt) in these cells, PLD activity was not affected by IL-1beta. Tetradecanoylphorbol Acetate 32-57 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 77-92 10527453-7 1999 However, despite the ability of phorbol myristate acetate (PMA) to stimulate phospholipase D (PLD) and synthesis of phosphatidylethanol (PEt) in these cells, PLD activity was not affected by IL-1beta. Tetradecanoylphorbol Acetate 32-57 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 94-97 10527453-7 1999 However, despite the ability of phorbol myristate acetate (PMA) to stimulate phospholipase D (PLD) and synthesis of phosphatidylethanol (PEt) in these cells, PLD activity was not affected by IL-1beta. Tetradecanoylphorbol Acetate 59-62 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 77-92 10527453-7 1999 However, despite the ability of phorbol myristate acetate (PMA) to stimulate phospholipase D (PLD) and synthesis of phosphatidylethanol (PEt) in these cells, PLD activity was not affected by IL-1beta. Tetradecanoylphorbol Acetate 59-62 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 94-97 10087440-6 1999 Activation of protein kinase C (PKC) by phorbol-12-myristate-13-acetate (PMA) increased PLD activity that was effectively blocked by the PKC inhibitors calphostin C (10(-8) to 10(-6) M) and GFX (10(-8) to 10(-6) M). Tetradecanoylphorbol Acetate 73-76 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 88-91 9873009-2 1999 In this study, we show that the secretion of matrix metalloproteinase-9 (MMP-9) from HT 1080 human fibrosarcoma cells was stimulated by phorbol 12-myristate 13-acetate in a time- and dose-dependent manner that involved protein kinase C. The phorbol ester also increased PLD activity in the cells. Tetradecanoylphorbol Acetate 136-167 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 270-273 9784628-4 1998 Phorbol myristate acetate-induced PLD activation was also inhibited in C3 exoenzyme-treated cells, but the inhibition was only partial. Tetradecanoylphorbol Acetate 0-25 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 34-37 10453985-4 1999 On the other hand, sphingosine at concentrations of 100-250 microM strongly stimulated PLD activity as compared to the effect of phorbol ester, 12-O-tetradecanoylphorbol 13-acetate (TPA), known as a PLD activator. Tetradecanoylphorbol Acetate 144-180 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 199-202 10453985-5 1999 The effect of TPA on PLD is linked to the activation of protein kinase C. The present study also shows that sphingosine additively enhances TPA-mediated PLD activity. Tetradecanoylphorbol Acetate 14-17 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 21-24 10453985-5 1999 The effect of TPA on PLD is linked to the activation of protein kinase C. The present study also shows that sphingosine additively enhances TPA-mediated PLD activity. Tetradecanoylphorbol Acetate 14-17 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 153-156 10453985-5 1999 The effect of TPA on PLD is linked to the activation of protein kinase C. The present study also shows that sphingosine additively enhances TPA-mediated PLD activity. Tetradecanoylphorbol Acetate 140-143 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 21-24 10453985-5 1999 The effect of TPA on PLD is linked to the activation of protein kinase C. The present study also shows that sphingosine additively enhances TPA-mediated PLD activity. Tetradecanoylphorbol Acetate 140-143 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 153-156 9601059-5 1998 PMA-stimulated PLD activity was blocked by the PKC inhibitor bisindolylmaleimide. Tetradecanoylphorbol Acetate 0-3 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 15-18 9346296-2 1997 In the present study, performed in cell-free preparations, we have characterized and compared the regulation of HEK cell PLD activity by the stable GTP analogue, guanosine 5"-O-[gamma-thio]triphosphate (GTP[S]), and the phorbol ester, phorbol 12-myristate 13-acetate (PMA). Tetradecanoylphorbol Acetate 235-266 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 121-124 9593849-1 1998 The protein kinase C (PKC) activator phorbol 12-myristate 13-acetate (PMA) stimulates both the synthesis and phospholipase D (PLD)-mediated hydrolysis of phosphatidylcholine (PtdCho). Tetradecanoylphorbol Acetate 37-68 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 109-124 9593849-1 1998 The protein kinase C (PKC) activator phorbol 12-myristate 13-acetate (PMA) stimulates both the synthesis and phospholipase D (PLD)-mediated hydrolysis of phosphatidylcholine (PtdCho). Tetradecanoylphorbol Acetate 37-68 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 126-129 9593849-1 1998 The protein kinase C (PKC) activator phorbol 12-myristate 13-acetate (PMA) stimulates both the synthesis and phospholipase D (PLD)-mediated hydrolysis of phosphatidylcholine (PtdCho). Tetradecanoylphorbol Acetate 70-73 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 109-124 9593849-1 1998 The protein kinase C (PKC) activator phorbol 12-myristate 13-acetate (PMA) stimulates both the synthesis and phospholipase D (PLD)-mediated hydrolysis of phosphatidylcholine (PtdCho). Tetradecanoylphorbol Acetate 70-73 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 126-129 9516439-6 1998 In contrast, PMA-induced PLD stimulation was inhibited by TcdB-1470 and TcsL in a time- and concentration-dependent manner, without alteration in immunologically detectable PKC isozyme levels. Tetradecanoylphorbol Acetate 13-16 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 25-28 9516439-8 1998 In contrast, pretreatment with TcdB-1470 and TcsL, but not TcdB, strongly reduced PMA-stimulated PLD activity. Tetradecanoylphorbol Acetate 82-85 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 97-100 9593849-4 1998 In multidrug resistant MCF-7/MDR1 cells, which highly express PKC-alpha but lack the PtdCho-specific PLD activity, 100-nM PMA had relatively small stimulatory effects on the uptake of [14C]choline (approximately 1.5-fold) and [14C]PtdCho synthesis (1.5- to 2-fold). Tetradecanoylphorbol Acetate 122-125 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 101-104 9593849-5 1998 In NIH 3T3 fibroblasts and MCF-7/PKC-alpha cells, both expressing PKC-alpha and PLD activities at high levels, 10-100-nM PMA enhanced [14C]choline uptake only slightly (1.7- to 2.2-fold), while it had much greater (approximately 4-9-fold) stimulatory effects on PtdCho synthesis. Tetradecanoylphorbol Acetate 121-124 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 80-83 9516474-6 1998 PMA also activates phospholipase D (PLD) in these cells and ethanol, a compound that inhibits PLD-mediated phosphatidic acid (PA) formation, blocked AA release. Tetradecanoylphorbol Acetate 0-3 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 19-34 9516474-6 1998 PMA also activates phospholipase D (PLD) in these cells and ethanol, a compound that inhibits PLD-mediated phosphatidic acid (PA) formation, blocked AA release. Tetradecanoylphorbol Acetate 0-3 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 36-39 9516474-6 1998 PMA also activates phospholipase D (PLD) in these cells and ethanol, a compound that inhibits PLD-mediated phosphatidic acid (PA) formation, blocked AA release. Tetradecanoylphorbol Acetate 0-3 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 94-97 9428812-5 1998 Phorbol 12-myristate 13-acetate (PMA) induced a prolonged activation of PLD, as detected in both intact cells and membranes. Tetradecanoylphorbol Acetate 0-31 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 72-75 9428812-5 1998 Phorbol 12-myristate 13-acetate (PMA) induced a prolonged activation of PLD, as detected in both intact cells and membranes. Tetradecanoylphorbol Acetate 33-36 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 72-75 9428812-9 1998 Ro-31-8220 and bisindolylmaleimide I, inhibitors of protein kinase C, blocked activation by PLD by both PMA and LPA. Tetradecanoylphorbol Acetate 104-107 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 92-95 9512645-2 1998 Membrane-associated phospholipase D (PLD) activity in response to guanosine 5"-O-(3-thiotriphosphate) (GTP gamma S) or phorbol myristate acetate (PMA) was upregulated by these treatments. Tetradecanoylphorbol Acetate 119-144 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 20-35 9512645-2 1998 Membrane-associated phospholipase D (PLD) activity in response to guanosine 5"-O-(3-thiotriphosphate) (GTP gamma S) or phorbol myristate acetate (PMA) was upregulated by these treatments. Tetradecanoylphorbol Acetate 119-144 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 37-40 9512645-2 1998 Membrane-associated phospholipase D (PLD) activity in response to guanosine 5"-O-(3-thiotriphosphate) (GTP gamma S) or phorbol myristate acetate (PMA) was upregulated by these treatments. Tetradecanoylphorbol Acetate 146-149 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 20-35 9512645-2 1998 Membrane-associated phospholipase D (PLD) activity in response to guanosine 5"-O-(3-thiotriphosphate) (GTP gamma S) or phorbol myristate acetate (PMA) was upregulated by these treatments. Tetradecanoylphorbol Acetate 146-149 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 37-40 9366247-5 1997 Moreover, in contrast to sphingosine which activates phospholipase D (PLD) leading to an increase in phosphatidic acid levels, sphingomyelinase, but not ceramide analogs, reduced TPA-stimulated PLD activity. Tetradecanoylphorbol Acetate 179-182 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 53-68 9366247-5 1997 Moreover, in contrast to sphingosine which activates phospholipase D (PLD) leading to an increase in phosphatidic acid levels, sphingomyelinase, but not ceramide analogs, reduced TPA-stimulated PLD activity. Tetradecanoylphorbol Acetate 179-182 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 70-73 9346296-2 1997 In the present study, performed in cell-free preparations, we have characterized and compared the regulation of HEK cell PLD activity by the stable GTP analogue, guanosine 5"-O-[gamma-thio]triphosphate (GTP[S]), and the phorbol ester, phorbol 12-myristate 13-acetate (PMA). Tetradecanoylphorbol Acetate 268-271 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 121-124 9144337-5 1997 Brief treatment of parathyroid and HEK-CaR cells with an activator of protein kinase C (PKC), phorbol 12-myristate,13-acetate (PMA), stimulated PLD activity at both low and high Ca2+(o). Tetradecanoylphorbol Acetate 127-130 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 144-147 9143362-2 1997 Treatment of permeabilized cells with phorbol myristate acetate (PMA) strongly potentiated GTP gamma S-dependent PLD activity at free Ca2+ < 100 nM. Tetradecanoylphorbol Acetate 38-63 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 113-116 9143362-2 1997 Treatment of permeabilized cells with phorbol myristate acetate (PMA) strongly potentiated GTP gamma S-dependent PLD activity at free Ca2+ < 100 nM. Tetradecanoylphorbol Acetate 65-68 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 113-116 9143362-3 1997 In the absence of GTP gamma S, PMA stimulated only minor PLD activity. Tetradecanoylphorbol Acetate 31-34 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 57-60 9144337-6 1997 Moreover, high Ca2+(o)-stimulated PLD activity was abolished following down-regulation of PKC by overnight phorbol myristate acetate (PMA) pretreatment, suggesting that CaR-mediated activation of PLD depends largely upon stimulation of PKC. Tetradecanoylphorbol Acetate 134-137 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 34-37 9144337-6 1997 Moreover, high Ca2+(o)-stimulated PLD activity was abolished following down-regulation of PKC by overnight phorbol myristate acetate (PMA) pretreatment, suggesting that CaR-mediated activation of PLD depends largely upon stimulation of PKC. Tetradecanoylphorbol Acetate 107-132 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 34-37 9144337-6 1997 Moreover, high Ca2+(o)-stimulated PLD activity was abolished following down-regulation of PKC by overnight phorbol myristate acetate (PMA) pretreatment, suggesting that CaR-mediated activation of PLD depends largely upon stimulation of PKC. Tetradecanoylphorbol Acetate 134-137 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 196-199 9142932-9 1997 Furthermore, downregulation of PKC by 12-O-tetradecanoylphorbol-13-acetate (100 nM, 18 h) also blocked polycation-mediated PLD stimulation. Tetradecanoylphorbol Acetate 38-74 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 123-126 9163343-2 1997 As part of an effort to identify the defect(s) in JB6 P- cells that might prevent the promoting effect of PMA, stimulation of phospholipase D (PLD)-mediated hydrolysis of phosphatidylcholine (PtdCho) and phosphatidylethanolamine (PtdEtn) by PMA as well as the rate of phospholipid synthesis were compared in three P+ variants, two P- variants and a transformed variant of the JB6 cell line. Tetradecanoylphorbol Acetate 106-109 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 126-141 9163343-2 1997 As part of an effort to identify the defect(s) in JB6 P- cells that might prevent the promoting effect of PMA, stimulation of phospholipase D (PLD)-mediated hydrolysis of phosphatidylcholine (PtdCho) and phosphatidylethanolamine (PtdEtn) by PMA as well as the rate of phospholipid synthesis were compared in three P+ variants, two P- variants and a transformed variant of the JB6 cell line. Tetradecanoylphorbol Acetate 106-109 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 143-146 9163343-2 1997 As part of an effort to identify the defect(s) in JB6 P- cells that might prevent the promoting effect of PMA, stimulation of phospholipase D (PLD)-mediated hydrolysis of phosphatidylcholine (PtdCho) and phosphatidylethanolamine (PtdEtn) by PMA as well as the rate of phospholipid synthesis were compared in three P+ variants, two P- variants and a transformed variant of the JB6 cell line. Tetradecanoylphorbol Acetate 241-244 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 126-141 9163343-2 1997 As part of an effort to identify the defect(s) in JB6 P- cells that might prevent the promoting effect of PMA, stimulation of phospholipase D (PLD)-mediated hydrolysis of phosphatidylcholine (PtdCho) and phosphatidylethanolamine (PtdEtn) by PMA as well as the rate of phospholipid synthesis were compared in three P+ variants, two P- variants and a transformed variant of the JB6 cell line. Tetradecanoylphorbol Acetate 241-244 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 143-146 8915773-9 1996 Phorbol 12-myristate 13-acetate (PMA) also stimulated PLD activity in human bone cells. Tetradecanoylphorbol Acetate 0-31 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 54-57 8890188-5 1996 Furthermore, the toxin B pretreatment also suppressed PLD activation induced by 4beta-phorbol 12-myristate 13-acetate in HL60 cell lysates. Tetradecanoylphorbol Acetate 80-117 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 54-57 8910539-1 1996 Stimulation of phospholipase D (PLD)-mediated hydrolysis of phosphatidylcholine (PtdCho) by phorbol 12-myristate 13-acetate (PMA) has been shown to be mediated by the alpha- and betaI-isoforms of protein kinase C (PKC). Tetradecanoylphorbol Acetate 92-123 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 15-30 8910539-1 1996 Stimulation of phospholipase D (PLD)-mediated hydrolysis of phosphatidylcholine (PtdCho) by phorbol 12-myristate 13-acetate (PMA) has been shown to be mediated by the alpha- and betaI-isoforms of protein kinase C (PKC). Tetradecanoylphorbol Acetate 92-123 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 32-35 8910539-4 1996 Stable expression of PKC-alpha in MCF-7 cells, which was accompanied by increased levels of the betaI- and theta-isoforms as well, greatly enhanced both PMA-induced PLD-mediated formation of phosphatidylethanol (approximately 5-fold) and the hydrolysis of PtdEtn (2.5-2.9-fold) and PtdCho (5.5-7.2-fold). Tetradecanoylphorbol Acetate 153-156 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 165-168 8915773-9 1996 Phorbol 12-myristate 13-acetate (PMA) also stimulated PLD activity in human bone cells. Tetradecanoylphorbol Acetate 33-36 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 54-57 8915773-11 1996 Acute pretreatment of cells with PMA reduced concomitantly the amounts of PKC alpha, but not of PKC epsilon, and the subsequent activation of PLD elicited by PKC activators. Tetradecanoylphorbol Acetate 33-36 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 142-145 8915773-14 1996 Moreover, PMA and NaF showed a supraadditive effect on PLD activation in Saos-2 cells. Tetradecanoylphorbol Acetate 10-13 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 55-58 8906581-5 1996 12-0-Tetradecanoylphorbol 13-acetate (TPA) stimulation activated a phospholipase D (PLD) specific for phosphatidylcholine (PtdCho) in proliferating cells and a phospholipase C (PLC) specific for phosphatidylethanolamine (PtdEtn) in retinoic acid (RA) differentiated cells. Tetradecanoylphorbol Acetate 38-41 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 67-82 8798752-2 1996 In intact HL-60 cells, phorbol myristate acetate (PMA) activated PLD as measured by [3H]palmitate-labeled phosphatidylcholine conversion to phosphatidylethanol in the presence of 2% ethanol. Tetradecanoylphorbol Acetate 23-48 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 65-68 8798752-2 1996 In intact HL-60 cells, phorbol myristate acetate (PMA) activated PLD as measured by [3H]palmitate-labeled phosphatidylcholine conversion to phosphatidylethanol in the presence of 2% ethanol. Tetradecanoylphorbol Acetate 50-53 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 65-68 8798752-5 1996 Although ceramide inhibited PMA-induced activation of PLD, it did not inhibit translocation of protein kinase C (PKC) to the membrane in response to PMA. Tetradecanoylphorbol Acetate 28-31 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 54-57 8906565-9 1996 A monoclonal antibody to phosphatidylinositol 4-kinase inhibits PIP2 synthesis in permeabilized U937 cells and blocks PLD activation by GTP gamma S and TPA. Tetradecanoylphorbol Acetate 152-155 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 118-121 8906581-5 1996 12-0-Tetradecanoylphorbol 13-acetate (TPA) stimulation activated a phospholipase D (PLD) specific for phosphatidylcholine (PtdCho) in proliferating cells and a phospholipase C (PLC) specific for phosphatidylethanolamine (PtdEtn) in retinoic acid (RA) differentiated cells. Tetradecanoylphorbol Acetate 38-41 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 84-87 8768715-5 1996 The PMA-induced generation of these three PLD products was inhibited by mepacrine which, in parallel, significantly blocked PMA-induced desensitization of [3H]LTB4 binding, which suggested that elevation of one or more of these products played a role in desensitization. Tetradecanoylphorbol Acetate 4-7 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 42-45 8898346-6 1996 TPA, a phorbol ester which induced a rapid and important redistribution of PKC, although unable to elicit PLC or PLA2 stimulation, specifically provoked PLD activation in a PKC-dependent but Ca(2+)-independent manner. Tetradecanoylphorbol Acetate 0-3 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 153-156 8768715-2 1996 In this paper, we demonstrate that the release of arachidonate from human polymorphonuclear leukocytes (PMNL) via a pathway initiated by phospholipase D (PLD) mediates phorbol myristate acetate (PMA)-induced desensitization of leukotriene B4 (LTB4) receptors. Tetradecanoylphorbol Acetate 168-193 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 137-152 8768715-2 1996 In this paper, we demonstrate that the release of arachidonate from human polymorphonuclear leukocytes (PMNL) via a pathway initiated by phospholipase D (PLD) mediates phorbol myristate acetate (PMA)-induced desensitization of leukotriene B4 (LTB4) receptors. Tetradecanoylphorbol Acetate 168-193 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 154-157 8804365-7 1996 The PLD activity was inhibited by reducing extracellular calcium (150 nM, 50% inhibition), exposure to 12-O-tetradecanoylphorbol 13 acetate (200 nM, 24 hours, 100% inhibition), Roche 31,8220 (10 microM, 15 minutes, 72% inhibition), or genestein (100 microM, 30 minutes, 56% inhibition), which suggests a dependence on both protein kinase C and tyrosine phosphorylation. Tetradecanoylphorbol Acetate 103-139 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 4-7 8768715-2 1996 In this paper, we demonstrate that the release of arachidonate from human polymorphonuclear leukocytes (PMNL) via a pathway initiated by phospholipase D (PLD) mediates phorbol myristate acetate (PMA)-induced desensitization of leukotriene B4 (LTB4) receptors. Tetradecanoylphorbol Acetate 195-198 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 137-152 8768715-5 1996 The PMA-induced generation of these three PLD products was inhibited by mepacrine which, in parallel, significantly blocked PMA-induced desensitization of [3H]LTB4 binding, which suggested that elevation of one or more of these products played a role in desensitization. Tetradecanoylphorbol Acetate 124-127 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 42-45 8768715-2 1996 In this paper, we demonstrate that the release of arachidonate from human polymorphonuclear leukocytes (PMNL) via a pathway initiated by phospholipase D (PLD) mediates phorbol myristate acetate (PMA)-induced desensitization of leukotriene B4 (LTB4) receptors. Tetradecanoylphorbol Acetate 195-198 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 154-157 7672124-1 1995 The tumor promoter phorbol 12-myristate 13-acetate (PMA) and hormonal activators of protein kinase C (PKC) commonly stimulate phospholipase D (PLD)-mediated formation of phosphatidic acid from phosphatidylcholine (PtdCho) in fibroblasts and other cell types. Tetradecanoylphorbol Acetate 19-50 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 126-141 8626786-2 1996 In HL60 cells, the membrane-bound phospholipase D (PLD) was stimulated by 4beta-phorbol 12-myristate 13-acetate (PMA) in the presence of the cytosolic fraction from HL60 cells or rat brain. Tetradecanoylphorbol Acetate 74-111 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 34-49 8626786-2 1996 In HL60 cells, the membrane-bound phospholipase D (PLD) was stimulated by 4beta-phorbol 12-myristate 13-acetate (PMA) in the presence of the cytosolic fraction from HL60 cells or rat brain. Tetradecanoylphorbol Acetate 74-111 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 51-54 8626786-2 1996 In HL60 cells, the membrane-bound phospholipase D (PLD) was stimulated by 4beta-phorbol 12-myristate 13-acetate (PMA) in the presence of the cytosolic fraction from HL60 cells or rat brain. Tetradecanoylphorbol Acetate 113-116 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 34-49 8626786-2 1996 In HL60 cells, the membrane-bound phospholipase D (PLD) was stimulated by 4beta-phorbol 12-myristate 13-acetate (PMA) in the presence of the cytosolic fraction from HL60 cells or rat brain. Tetradecanoylphorbol Acetate 113-116 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 51-54 8579603-3 1996 The arsenical compound, phenylarsine oxide (PAO), which reacts with vicinal sulhydryl groups, activated twofold at one minute the PMA stimulated-PLD activity, whereas it inhibited half of the fMLP-activated PLD after a time lag of 30 sec. Tetradecanoylphorbol Acetate 130-133 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 145-148 8579603-3 1996 The arsenical compound, phenylarsine oxide (PAO), which reacts with vicinal sulhydryl groups, activated twofold at one minute the PMA stimulated-PLD activity, whereas it inhibited half of the fMLP-activated PLD after a time lag of 30 sec. Tetradecanoylphorbol Acetate 130-133 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 207-210 8555272-13 1996 Furthermore, PMA-stimulated PLD activity was also significantly reduced by a preincubation of PMN with C2-ceramide. Tetradecanoylphorbol Acetate 13-16 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 28-31 7672124-1 1995 The tumor promoter phorbol 12-myristate 13-acetate (PMA) and hormonal activators of protein kinase C (PKC) commonly stimulate phospholipase D (PLD)-mediated formation of phosphatidic acid from phosphatidylcholine (PtdCho) in fibroblasts and other cell types. Tetradecanoylphorbol Acetate 19-50 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 143-146 7672124-1 1995 The tumor promoter phorbol 12-myristate 13-acetate (PMA) and hormonal activators of protein kinase C (PKC) commonly stimulate phospholipase D (PLD)-mediated formation of phosphatidic acid from phosphatidylcholine (PtdCho) in fibroblasts and other cell types. Tetradecanoylphorbol Acetate 52-55 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 126-141 7672124-1 1995 The tumor promoter phorbol 12-myristate 13-acetate (PMA) and hormonal activators of protein kinase C (PKC) commonly stimulate phospholipase D (PLD)-mediated formation of phosphatidic acid from phosphatidylcholine (PtdCho) in fibroblasts and other cell types. Tetradecanoylphorbol Acetate 52-55 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 143-146 8527309-2 1995 The effect of 17 beta-oestradiol on PAF- and 12-O-tetradecanoylphorbol 13-acetate (TPA)-evoked PLD activity assayed as an accumulation of [3H]phosphatidylbutanol was examined in [3H]myristic acid labelled in a human endometrial epithelial cell line HEC-1B. Tetradecanoylphorbol Acetate 45-81 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 95-98 7544577-5 1995 Phorbol 12-myristate 13-acetate (PMA), a direct activator of protein kinase C, also increased PLD activity. Tetradecanoylphorbol Acetate 0-31 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 94-97 7544577-5 1995 Phorbol 12-myristate 13-acetate (PMA), a direct activator of protein kinase C, also increased PLD activity. Tetradecanoylphorbol Acetate 33-36 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 94-97 8588976-2 1995 In [3H]myristate-labelled endometrial stromal cells, bradykinin and tetradecanoylphorbol acetate (TPA) mediated activation of phospholipase D (PLD) as measured by the accumulation of [3H]phosphatidylbutanol ([3H]PtdBut). Tetradecanoylphorbol Acetate 68-96 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 126-141 8588976-2 1995 In [3H]myristate-labelled endometrial stromal cells, bradykinin and tetradecanoylphorbol acetate (TPA) mediated activation of phospholipase D (PLD) as measured by the accumulation of [3H]phosphatidylbutanol ([3H]PtdBut). Tetradecanoylphorbol Acetate 68-96 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 143-146 8588976-2 1995 In [3H]myristate-labelled endometrial stromal cells, bradykinin and tetradecanoylphorbol acetate (TPA) mediated activation of phospholipase D (PLD) as measured by the accumulation of [3H]phosphatidylbutanol ([3H]PtdBut). Tetradecanoylphorbol Acetate 98-101 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 126-141 8588976-2 1995 In [3H]myristate-labelled endometrial stromal cells, bradykinin and tetradecanoylphorbol acetate (TPA) mediated activation of phospholipase D (PLD) as measured by the accumulation of [3H]phosphatidylbutanol ([3H]PtdBut). Tetradecanoylphorbol Acetate 98-101 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 143-146 8588976-6 1995 Chronic pretreatment with 400 nM TPA abolished PLD activation to subsequent treatment with either TPA and bradykinin. Tetradecanoylphorbol Acetate 33-36 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 47-50 8588976-8 1995 The effect of bradykinin and TPA on PLD activity was synergistic, suggesting that the two agents may act via different mechanisms. Tetradecanoylphorbol Acetate 29-32 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 36-39 7779100-2 1995 The activation of membrane-bound PLD by PKC partially purified from rat brain was most effectively induced with phorbol 12-myristate 13-acetate (PMA) and Ca2+ (1 microM) which caused translocation of PKC to membranes. Tetradecanoylphorbol Acetate 112-143 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 33-36 7779100-2 1995 The activation of membrane-bound PLD by PKC partially purified from rat brain was most effectively induced with phorbol 12-myristate 13-acetate (PMA) and Ca2+ (1 microM) which caused translocation of PKC to membranes. Tetradecanoylphorbol Acetate 145-148 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 33-36 8527309-5 1995 In the 17 beta-oestradiol-treated cells, TPA-stimulated PLD activity was significantly elevated at 100 nM TPA (P < 0.05) and 1 microM TPA (P < 0.05) compared to responses in the untreated cells, suggesting that 17 beta-oestradiol may upregulate PKC activity. Tetradecanoylphorbol Acetate 106-109 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 56-59 8527309-5 1995 In the 17 beta-oestradiol-treated cells, TPA-stimulated PLD activity was significantly elevated at 100 nM TPA (P < 0.05) and 1 microM TPA (P < 0.05) compared to responses in the untreated cells, suggesting that 17 beta-oestradiol may upregulate PKC activity. Tetradecanoylphorbol Acetate 106-109 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 56-59 8527309-7 1995 TPA (10 nM) and PAF (100 nM) stimulated PLD activity. Tetradecanoylphorbol Acetate 0-3 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 40-43 8527309-2 1995 The effect of 17 beta-oestradiol on PAF- and 12-O-tetradecanoylphorbol 13-acetate (TPA)-evoked PLD activity assayed as an accumulation of [3H]phosphatidylbutanol was examined in [3H]myristic acid labelled in a human endometrial epithelial cell line HEC-1B. Tetradecanoylphorbol Acetate 83-86 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 95-98 8527309-8 1995 However, TPA-stimulated PLD activity levels fell 10-fold while PAF-mediated PLD activity remained elevated at 10 nM and 100 nM concentrations of 17 beta-oestradiol suggesting a different mechanism of activation. Tetradecanoylphorbol Acetate 9-12 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 24-27 8527309-3 1995 TPA stimulated PLD activity in a dose-dependent manner whereas PAF had no significant effect on PLD activity. Tetradecanoylphorbol Acetate 0-3 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 15-18 8527309-5 1995 In the 17 beta-oestradiol-treated cells, TPA-stimulated PLD activity was significantly elevated at 100 nM TPA (P < 0.05) and 1 microM TPA (P < 0.05) compared to responses in the untreated cells, suggesting that 17 beta-oestradiol may upregulate PKC activity. Tetradecanoylphorbol Acetate 41-44 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 56-59 7944397-4 1994 Of the two major substrates of PLD, phosphatidylcholine (PtdCho) and phosphatidylethanolamine (PtdEtn), vitamin K3 (10-100 microM) preferentially inhibited PtdEtn hydrolysis when stimulated by PMA or platelet-derived growth factor, the latter being a hormonal activator of PKC. Tetradecanoylphorbol Acetate 193-196 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 31-34 7890622-10 1995 In parallel, the anti-PtdIns 4-kinase fully inhibited the activation of PLD by GTP gamma S and caused a 60% inhibition of PLD activation by the phorbol ester 12-O-tetradecanoylphorbol-13-acetate, indicating that elevated PtdIns-4,5-P2 levels are required for PLD activation. Tetradecanoylphorbol Acetate 158-194 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 122-125 7890622-10 1995 In parallel, the anti-PtdIns 4-kinase fully inhibited the activation of PLD by GTP gamma S and caused a 60% inhibition of PLD activation by the phorbol ester 12-O-tetradecanoylphorbol-13-acetate, indicating that elevated PtdIns-4,5-P2 levels are required for PLD activation. Tetradecanoylphorbol Acetate 158-194 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 122-125 11550712-6 1994 The PKC inhibitor, staurosporine, as well as PKC downregulation, were both found to inhibit PMA-induced PLD activity without inhibiting other PMA-induced effects in chondrocytes. Tetradecanoylphorbol Acetate 92-95 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 104-107 7945188-6 1994 These data indicate that MCF-7/MDR cells contain a PtdEtn-specific PLD activity which can be selectively stimulated by PMA, sphingosine and H2O2. Tetradecanoylphorbol Acetate 119-122 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 67-70 8304417-2 1994 The ability of bFGF and 12-O-tetradecanoylphorbol-13-acetate (TPA) to stimulate PLD activity was completely abolished in cells pretreated with 400 nM TPA for 48 h to downregulate protein kinase C (PKC). Tetradecanoylphorbol Acetate 24-60 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 80-83 7935319-5 1994 In contrast to the desensitization observed with agonists, phorbol-12-myristate-13-acetate induces a sustained stimulation of PLD. Tetradecanoylphorbol Acetate 59-90 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 126-129 8011674-3 1994 LPS-mediated PLD activation of THP-1 or U-937 was inhibited by staurosporine (2 microM) and by protein kinase C (PKC) down-regulation with 12-O-tetradecanoylphorbol 13-acetate (TPA) suggesting a role for PKC. Tetradecanoylphorbol Acetate 139-175 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 13-16 8011674-3 1994 LPS-mediated PLD activation of THP-1 or U-937 was inhibited by staurosporine (2 microM) and by protein kinase C (PKC) down-regulation with 12-O-tetradecanoylphorbol 13-acetate (TPA) suggesting a role for PKC. Tetradecanoylphorbol Acetate 177-180 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 13-16 7909661-4 1994 Dexamethasone inhibited the DMSO-induced increase of CD11b cell surface expression as well as the oxidative response and PLD activation triggered by 4 beta-phorbol 12-myristate 13-acetate. Tetradecanoylphorbol Acetate 151-187 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 121-124 8129736-1 1994 Protein kinase C (PKC), an enzyme which is believed to mediate the stimulatory effects of the PKC activator phorbol 12-myristate 13-acetate (PMA) on phospholipase D (PLD) activity, has a zinc-dependent structure required for phorbol ester binding. Tetradecanoylphorbol Acetate 108-139 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 149-164 8129736-1 1994 Protein kinase C (PKC), an enzyme which is believed to mediate the stimulatory effects of the PKC activator phorbol 12-myristate 13-acetate (PMA) on phospholipase D (PLD) activity, has a zinc-dependent structure required for phorbol ester binding. Tetradecanoylphorbol Acetate 108-139 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 166-169 8129736-1 1994 Protein kinase C (PKC), an enzyme which is believed to mediate the stimulatory effects of the PKC activator phorbol 12-myristate 13-acetate (PMA) on phospholipase D (PLD) activity, has a zinc-dependent structure required for phorbol ester binding. Tetradecanoylphorbol Acetate 141-144 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 149-164 8129736-1 1994 Protein kinase C (PKC), an enzyme which is believed to mediate the stimulatory effects of the PKC activator phorbol 12-myristate 13-acetate (PMA) on phospholipase D (PLD) activity, has a zinc-dependent structure required for phorbol ester binding. Tetradecanoylphorbol Acetate 141-144 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 166-169 8129736-4 1994 In [14C]palmitic acid-labelled fibroblasts, in the presence of ethanol, phenanthroline also enhanced the stimulatory effect of PMA on the synthesis of phosphatidylethanol, a marker of PLD activity. Tetradecanoylphorbol Acetate 127-130 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 184-187 8304417-2 1994 The ability of bFGF and 12-O-tetradecanoylphorbol-13-acetate (TPA) to stimulate PLD activity was completely abolished in cells pretreated with 400 nM TPA for 48 h to downregulate protein kinase C (PKC). Tetradecanoylphorbol Acetate 62-65 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 80-83 8304417-2 1994 The ability of bFGF and 12-O-tetradecanoylphorbol-13-acetate (TPA) to stimulate PLD activity was completely abolished in cells pretreated with 400 nM TPA for 48 h to downregulate protein kinase C (PKC). Tetradecanoylphorbol Acetate 150-153 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 80-83 8383432-15 1993 Taken together, these data suggest that glucose differentially interferes with activation of PLD but not phospholipase C. And, the fact that PMA-induced activation of PLD is not altered by glucose further suggests that a protein kinase C independent step leading to the activation of PLD may be altered by glucose. Tetradecanoylphorbol Acetate 141-144 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 93-96 8344313-1 1993 The activation of phospholipase D (PLD) by platelet-derived growth factor (PDGF), prostaglandin F2 alpha and 12-O-tetradecanoylphorbol 13-acetate (TPA) was studied in NIH-3T3 fibroblasts. Tetradecanoylphorbol Acetate 109-145 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 18-33 8344313-1 1993 The activation of phospholipase D (PLD) by platelet-derived growth factor (PDGF), prostaglandin F2 alpha and 12-O-tetradecanoylphorbol 13-acetate (TPA) was studied in NIH-3T3 fibroblasts. Tetradecanoylphorbol Acetate 109-145 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 35-38 8344313-1 1993 The activation of phospholipase D (PLD) by platelet-derived growth factor (PDGF), prostaglandin F2 alpha and 12-O-tetradecanoylphorbol 13-acetate (TPA) was studied in NIH-3T3 fibroblasts. Tetradecanoylphorbol Acetate 147-150 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 18-33 8344313-1 1993 The activation of phospholipase D (PLD) by platelet-derived growth factor (PDGF), prostaglandin F2 alpha and 12-O-tetradecanoylphorbol 13-acetate (TPA) was studied in NIH-3T3 fibroblasts. Tetradecanoylphorbol Acetate 147-150 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 35-38 8344313-5 1993 In contrast, TPA-induced activation of PLD was sustained for at least 60 min of incubation. Tetradecanoylphorbol Acetate 13-16 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 39-42 8344313-6 1993 A combination of maximally effective concentrations of PDGF and TPA stimulated PLD activity in a non-additive manner, while the effect of prostaglandin F2 alpha was additional to that of either PDGF or TPA. Tetradecanoylphorbol Acetate 64-67 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 79-82 8344313-7 1993 The protein kinase inhibitor staurosporine inhibited PLD activation by PDGF or TPA with almost identical dose/response curves. Tetradecanoylphorbol Acetate 79-82 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 53-56 8344313-9 1993 The specific protein kinase C inhibitor GF109203X (a bisindolylmaleimide) inhibited PLD activation by prostaglandin F2 alpha and PDGF at concentrations higher than those required for inhibition of PLD activation induced by TPA. Tetradecanoylphorbol Acetate 223-226 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 84-87 8504157-3 1993 The PLD was shown to be activated by phorbol, 12-myristate, 13-acetate (PMA), calcium ionophore A23187, oxytocin, bombesin and bradykinin, but not by platelet-activating factor (PAF) and epidermal growth factor (EGF). Tetradecanoylphorbol Acetate 72-75 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 4-7 8504157-8 1993 The PA synthesis caused by the two stimulators was similarly inhibited by staurosporine and by a chronic treatment with PMA (100 nM for 24 h), suggesting that the activation of PLD is linked to the action of protein kinase C. With the cells labeled with radioactive choline and ethanolamine, we found that the amniotic PLD hydrolyzed almost equally phosphatidylcholine and phosphatidylethanolamine. Tetradecanoylphorbol Acetate 120-123 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 177-180 8504157-8 1993 The PA synthesis caused by the two stimulators was similarly inhibited by staurosporine and by a chronic treatment with PMA (100 nM for 24 h), suggesting that the activation of PLD is linked to the action of protein kinase C. With the cells labeled with radioactive choline and ethanolamine, we found that the amniotic PLD hydrolyzed almost equally phosphatidylcholine and phosphatidylethanolamine. Tetradecanoylphorbol Acetate 120-123 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 319-322 8383432-15 1993 Taken together, these data suggest that glucose differentially interferes with activation of PLD but not phospholipase C. And, the fact that PMA-induced activation of PLD is not altered by glucose further suggests that a protein kinase C independent step leading to the activation of PLD may be altered by glucose. Tetradecanoylphorbol Acetate 141-144 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 167-170 8383432-15 1993 Taken together, these data suggest that glucose differentially interferes with activation of PLD but not phospholipase C. And, the fact that PMA-induced activation of PLD is not altered by glucose further suggests that a protein kinase C independent step leading to the activation of PLD may be altered by glucose. Tetradecanoylphorbol Acetate 141-144 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 167-170 1330154-11 1992 Preincubation with the PKC inhibitor Ro-31-8220 abolished both TPA- and vasopressin-stimulated [3H]-PtdBuOH, suggesting that the intermediate activation of protein kinase C is involved in the regulation of PLD by vasopressin. Tetradecanoylphorbol Acetate 63-66 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 206-209 1472000-7 1992 Similarly to sphingosine, the protein phosphatase inhibitor okadaic acid also had either potentiating or inhibitory effects on PMA-stimulated PLD activity, depending on the length of incubation time and the concentration of PMA. Tetradecanoylphorbol Acetate 127-130 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 142-145 1472000-7 1992 Similarly to sphingosine, the protein phosphatase inhibitor okadaic acid also had either potentiating or inhibitory effects on PMA-stimulated PLD activity, depending on the length of incubation time and the concentration of PMA. Tetradecanoylphorbol Acetate 224-227 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 142-145 1472000-8 1992 Consistent with the presence of an inhibitory component in the overall action of PKC, the PKC inhibitor staurosporine and down-regulation of PKC activity by prolonged (24 h) treatment with PMA similarly enhanced PLD activity. Tetradecanoylphorbol Acetate 189-192 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 212-215 1482699-1 1992 In a previous study, ethanol was shown to enhance the stimulatory effect of phorbol 12-myristate 13-acetate (PMA), a prominent activator of protein kinase C (PKC), on phospholipase-D (PLD)-mediated hydrolysis of phosphatidylethanolamine (PtdEtn) in NIH 3T3 fibroblasts (Kiss et al. Tetradecanoylphorbol Acetate 76-107 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 167-182 1482699-1 1992 In a previous study, ethanol was shown to enhance the stimulatory effect of phorbol 12-myristate 13-acetate (PMA), a prominent activator of protein kinase C (PKC), on phospholipase-D (PLD)-mediated hydrolysis of phosphatidylethanolamine (PtdEtn) in NIH 3T3 fibroblasts (Kiss et al. Tetradecanoylphorbol Acetate 76-107 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 184-187 1482699-1 1992 In a previous study, ethanol was shown to enhance the stimulatory effect of phorbol 12-myristate 13-acetate (PMA), a prominent activator of protein kinase C (PKC), on phospholipase-D (PLD)-mediated hydrolysis of phosphatidylethanolamine (PtdEtn) in NIH 3T3 fibroblasts (Kiss et al. Tetradecanoylphorbol Acetate 109-112 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 167-182 1482699-1 1992 In a previous study, ethanol was shown to enhance the stimulatory effect of phorbol 12-myristate 13-acetate (PMA), a prominent activator of protein kinase C (PKC), on phospholipase-D (PLD)-mediated hydrolysis of phosphatidylethanolamine (PtdEtn) in NIH 3T3 fibroblasts (Kiss et al. Tetradecanoylphorbol Acetate 109-112 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 184-187 1419487-1 1992 The addition of granulocyte-macrophage colony-stimulating factor (GM-CSF) to human peripheral blood neutrophils primes phospholipase D (PLD) to subsequent stimulation by N-formyl-methionyl-leucyl-phenylalanine (fMLP) or phorbol myristate acetate (PMA). Tetradecanoylphorbol Acetate 220-245 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 119-134 1419487-1 1992 The addition of granulocyte-macrophage colony-stimulating factor (GM-CSF) to human peripheral blood neutrophils primes phospholipase D (PLD) to subsequent stimulation by N-formyl-methionyl-leucyl-phenylalanine (fMLP) or phorbol myristate acetate (PMA). Tetradecanoylphorbol Acetate 220-245 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 136-139 1419487-1 1992 The addition of granulocyte-macrophage colony-stimulating factor (GM-CSF) to human peripheral blood neutrophils primes phospholipase D (PLD) to subsequent stimulation by N-formyl-methionyl-leucyl-phenylalanine (fMLP) or phorbol myristate acetate (PMA). Tetradecanoylphorbol Acetate 247-250 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 119-134 1419487-1 1992 The addition of granulocyte-macrophage colony-stimulating factor (GM-CSF) to human peripheral blood neutrophils primes phospholipase D (PLD) to subsequent stimulation by N-formyl-methionyl-leucyl-phenylalanine (fMLP) or phorbol myristate acetate (PMA). Tetradecanoylphorbol Acetate 247-250 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 136-139 1908735-12 1991 Demethoxyviridin also inhibited PLD activation induced by sodium fluoride or phorbol myristate acetate (PMA) but the concentrations required were 100 times those needed to block fMet-Leu-Phe-stimulated PLD. Tetradecanoylphorbol Acetate 77-102 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 32-35 2037586-12 1991 The protein kinase C (PKC) inhibitors staurosporin and 1-O-hexadecyl-2-O-methylglycerol inhibited BK- and PMA-induced activation of PLD. Tetradecanoylphorbol Acetate 106-109 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 132-135 1599436-5 1992 Phorbol 12-myristate 13-acetate or Ca2+ alone can also stimulate PLD, but to a limited extent. Tetradecanoylphorbol Acetate 0-31 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 65-68 1659382-8 1991 Down-regulation of protein kinase C (PKC), by pre-treatment with phorbol 12-myristate 13-acetate, abolished both [3H]choline and [3H]PtdBut formation, suggesting that PLD-catalysed PtdCho hydrolysis may be dependent on PKC activation, supporting its dependence on prior PtdIns(4,5)P2 hydrolysis. Tetradecanoylphorbol Acetate 65-96 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 167-170 1908735-12 1991 Demethoxyviridin also inhibited PLD activation induced by sodium fluoride or phorbol myristate acetate (PMA) but the concentrations required were 100 times those needed to block fMet-Leu-Phe-stimulated PLD. Tetradecanoylphorbol Acetate 104-107 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 32-35 2222456-8 1990 Interestingly, TPA activated PLD in intact cells but not in lysates or subcellular fractions. Tetradecanoylphorbol Acetate 15-18 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 29-32 2222456-9 1990 These observations suggest that stimulation of PLD-catalyzed PEt synthesis by TPA is not solely mediated through PKC activation. Tetradecanoylphorbol Acetate 78-81 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 47-50 2130511-18 1990 Activation of phospholipase D (PLD) was demonstrated by the finding that phosphatidic acid increased in response to PMA or carbachol prior to the increase in PA. Tetradecanoylphorbol Acetate 116-119 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 14-29 2390085-0 1990 Phorbol-12-myristate-13-acetate activation of phospholipase D in human neutrophils leads to the production of phosphatides and diglycerides. Tetradecanoylphorbol Acetate 0-31 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 46-61 2390085-1 1990 The contribution of phospholipase D (PLD) to the production of phosphatides (PA) and diglycerides (DG) in phorbol-12-myristate-13-acetate (PMA)-stimulated human neutrophils was studied. Tetradecanoylphorbol Acetate 106-137 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 20-35 2390085-1 1990 The contribution of phospholipase D (PLD) to the production of phosphatides (PA) and diglycerides (DG) in phorbol-12-myristate-13-acetate (PMA)-stimulated human neutrophils was studied. Tetradecanoylphorbol Acetate 106-137 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 37-40 2390085-1 1990 The contribution of phospholipase D (PLD) to the production of phosphatides (PA) and diglycerides (DG) in phorbol-12-myristate-13-acetate (PMA)-stimulated human neutrophils was studied. Tetradecanoylphorbol Acetate 139-142 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 20-35 2390085-1 1990 The contribution of phospholipase D (PLD) to the production of phosphatides (PA) and diglycerides (DG) in phorbol-12-myristate-13-acetate (PMA)-stimulated human neutrophils was studied. Tetradecanoylphorbol Acetate 139-142 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 37-40 2390085-7 1990 The results of this study demonstrate that PMA activates PLD in neutrophils leading to the generation of PA and that the bulk of the DG mass accumulation is derived from the sequential actions of PLD and PPH on PC. Tetradecanoylphorbol Acetate 43-46 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 57-60 2130511-18 1990 Activation of phospholipase D (PLD) was demonstrated by the finding that phosphatidic acid increased in response to PMA or carbachol prior to the increase in PA. Tetradecanoylphorbol Acetate 116-119 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 31-34 2498324-11 1989 Based on experiments with both [3H]alkyl-PC and alkyl-[32P]PC, it is concluded that alkyl-PA and alkyl-PEt formed in response to PMA, OAG, or A23187 are derived exclusively from PLD action on alkyl-PC. Tetradecanoylphorbol Acetate 129-132 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 178-181 8070364-4 1994 PLD was also activated by 12-O-tetradecanoylphorbol-13-acetate (TPA). Tetradecanoylphorbol Acetate 26-62 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 0-3 8070364-4 1994 PLD was also activated by 12-O-tetradecanoylphorbol-13-acetate (TPA). Tetradecanoylphorbol Acetate 64-67 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 0-3 2498324-12 1989 Furthermore, subthreshold concentrations of PMA (0.5-2.0 nM) or OAG (1.0-25 microM) combined with subthreshold levels of A23187 (15-60 nM) induce the formation of alkyl-[32P]PA and alkyl-[32P]PEt, suggesting that receptor-mediated activation of PLD might involve cooperative interactions between Ca2+ and diglyceride. Tetradecanoylphorbol Acetate 44-47 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 245-248