PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 16472984-4 2006 RESULTS: Levels of IL-2 mRNA in phorbol 12-myristate 13-acetate/ionomycin activated splenocytes and cytokine in T-helper-1 cells were increased by 50 microM of alphaTOC but decreased by 1 mM of alphaTOC. Tetradecanoylphorbol Acetate 32-63 interleukin 2 Mus musculus 19-23 17630204-4 2007 Isoeugenol inhibited phorbol 12-myristate 13-acetate (PMA) plus ionomycin (Io)-induced IL-2 mRNA expression and protein secretion in B6C3F1 mouse splenocytes, and in EL4.IL-2 mouse T-cells, as determined by real-time RT-PCR and ELISA, respectively. Tetradecanoylphorbol Acetate 21-52 interleukin 2 Mus musculus 87-91 17630204-4 2007 Isoeugenol inhibited phorbol 12-myristate 13-acetate (PMA) plus ionomycin (Io)-induced IL-2 mRNA expression and protein secretion in B6C3F1 mouse splenocytes, and in EL4.IL-2 mouse T-cells, as determined by real-time RT-PCR and ELISA, respectively. Tetradecanoylphorbol Acetate 21-52 interleukin 2 Mus musculus 170-174 17630204-4 2007 Isoeugenol inhibited phorbol 12-myristate 13-acetate (PMA) plus ionomycin (Io)-induced IL-2 mRNA expression and protein secretion in B6C3F1 mouse splenocytes, and in EL4.IL-2 mouse T-cells, as determined by real-time RT-PCR and ELISA, respectively. Tetradecanoylphorbol Acetate 54-57 interleukin 2 Mus musculus 170-174 17531200-5 2007 Cabin1 (701-900) blocked both CN-mediated dephosphorylation and nuclear import of NFAT and thus inhibited IL-2 production in response to PMA/ionomycin stimulation. Tetradecanoylphorbol Acetate 137-140 interleukin 2 Mus musculus 106-110 17438683-9 2006 The levels of IL-10, and especially IL-2 were elevated by TPA, but they were markedly lower than that in control group after PD098059 pretreatment. Tetradecanoylphorbol Acetate 58-61 interleukin 2 Mus musculus 36-40 17438683-12 2006 TPA and A23187 can markedly rectify the disturbance of IL-2/IL-10 secretion ratio by increasing the IL-2 secretion after scald. Tetradecanoylphorbol Acetate 0-3 interleukin 2 Mus musculus 55-59 17438683-12 2006 TPA and A23187 can markedly rectify the disturbance of IL-2/IL-10 secretion ratio by increasing the IL-2 secretion after scald. Tetradecanoylphorbol Acetate 0-3 interleukin 2 Mus musculus 100-104 16531003-4 2006 Anethole inhibited phorbol 12-myristate 13-acetate (PMA) plus ionomycin (Io)-induced interleukin-2 (IL-2) mRNA expression and protein secretion in EL4 mouse T-cells as determined by quantitative/competitive RT-PCR and ELISA, respectively. Tetradecanoylphorbol Acetate 19-50 interleukin 2 Mus musculus 85-98 16531003-4 2006 Anethole inhibited phorbol 12-myristate 13-acetate (PMA) plus ionomycin (Io)-induced interleukin-2 (IL-2) mRNA expression and protein secretion in EL4 mouse T-cells as determined by quantitative/competitive RT-PCR and ELISA, respectively. Tetradecanoylphorbol Acetate 19-50 interleukin 2 Mus musculus 100-104 12091710-6 2002 Total peripheral T cell numbers were significantly reduced in CnA beta(-/-) mice and were defective in proliferative capacity and IL-2 production in response to PMA/ionomycin and T cell receptor cross-linking. Tetradecanoylphorbol Acetate 161-164 interleukin 2 Mus musculus 130-134 12183516-5 2002 Examination of the ability of splenocytes from uninfected and infected mice to produce IFN-gamma revealed that IL-2(-/-) mice were hyporesponsive to stimulation with anti-CD3 or parasite antigen compared with wild-type mice, and the addition of IL-2 alone or in combination with IL-12 or stimulation with phorbol myristate acetate and ionomycin did not restore the production of IFN-gamma. Tetradecanoylphorbol Acetate 305-330 interleukin 2 Mus musculus 111-115 11809870-3 2002 We demonstrate here that steady-state IL-2 mRNA expression was significantly enhanced by CBN in a concentration-dependent manner in EL4 cells activated with suboptimal concentrations of phorbol-12-myristate-13-acetate (2-10 nM). Tetradecanoylphorbol Acetate 186-217 interleukin 2 Mus musculus 38-42 10479400-8 1999 PD98059 also inhibited induction of IL-2 by phorbol 12-myristate 13-acetate (PMA), and AP1-linked reporter gene expression in response to PMA but not IL-1. Tetradecanoylphorbol Acetate 44-75 interleukin 2 Mus musculus 36-40 11125308-3 2000 Concanavalin A or phorbol myristate acetate/calcium ionophore/anti-CD3 stimulation of spleen cells from H2(b) congenic mice induced less IL-1, IL-2, IFN-gamma and MIF mRNA and/or protein than the equivalent cells from H2(d) mice. Tetradecanoylphorbol Acetate 18-43 interleukin 2 Mus musculus 143-147 10640296-1 2000 We previously reported that immunosuppressive cannabinoids inhibited interleukin (IL)-2 steady-state mRNA expression and secretion by phorbol-12-myristate-13-acetate plus ionomycin-activated mouse splenocytes and EL4 murine T-cells. Tetradecanoylphorbol Acetate 134-165 interleukin 2 Mus musculus 69-87 31207793-9 1997 Upon stimulation of EL4.IL-2 cells with phorbol 12-myristate-13-acetate, IL-2 secretion increased up to 1.9-fold in the presence of 106 L. bulgaricus Lr 79 cells per ml whereas this cytokine decreased in the presence of 107 or 108 lactobacilli per ml. Tetradecanoylphorbol Acetate 40-71 interleukin 2 Mus musculus 24-28 10472804-2 1999 AFB1 inhibited the phorbol-12myristate-13-acetate/i6nomycin (PMA/Io)-induced IL-2 mRNA expression in the murine thymocytes and Jurkat E6-1 cells as determined by qualitative RT-PCR, while no effect was observed in the EL4.IL-2 cells. Tetradecanoylphorbol Acetate 19-49 interleukin 2 Mus musculus 77-81 10472804-2 1999 AFB1 inhibited the phorbol-12myristate-13-acetate/i6nomycin (PMA/Io)-induced IL-2 mRNA expression in the murine thymocytes and Jurkat E6-1 cells as determined by qualitative RT-PCR, while no effect was observed in the EL4.IL-2 cells. Tetradecanoylphorbol Acetate 19-49 interleukin 2 Mus musculus 222-226 10472804-2 1999 AFB1 inhibited the phorbol-12myristate-13-acetate/i6nomycin (PMA/Io)-induced IL-2 mRNA expression in the murine thymocytes and Jurkat E6-1 cells as determined by qualitative RT-PCR, while no effect was observed in the EL4.IL-2 cells. Tetradecanoylphorbol Acetate 61-64 interleukin 2 Mus musculus 77-81 10472804-2 1999 AFB1 inhibited the phorbol-12myristate-13-acetate/i6nomycin (PMA/Io)-induced IL-2 mRNA expression in the murine thymocytes and Jurkat E6-1 cells as determined by qualitative RT-PCR, while no effect was observed in the EL4.IL-2 cells. Tetradecanoylphorbol Acetate 61-64 interleukin 2 Mus musculus 222-226 10410976-7 1999 Polyclonal activation of lung T cells from OA/OA mice with 12-myristate 13-acetate (PMA), ionomycin, anti-CD3 mAb, and anti-CD28 mAb resulted in higher percentages of IL-2+ (43%) and IL-5+ (7%) CD4 cells when compared to CD4+ T cells from non-OA sensitized, challenged mice. Tetradecanoylphorbol Acetate 84-87 interleukin 2 Mus musculus 167-171 9808167-5 1998 The signal transmitting function of CD73 is also conserved, as splenic T cells treated with anti-CD73 mAb plus phorbol 12-myristate 13-acetate proliferate and secrete IL-2. Tetradecanoylphorbol Acetate 111-142 interleukin 2 Mus musculus 167-171 9645418-2 1998 When the peripheral mononuclear cells were stimulated with phorbol myristate acetate and ionomycin in the presence of monensin, which blocks the secretion of cytokines, the positive rates for the cytoplasmic IL-2 and IFN-gamma were lower and those for the cytoplasmic IL-4 and IL-10 were higher in SLE than in normal subjects. Tetradecanoylphorbol Acetate 59-84 interleukin 2 Mus musculus 208-212 9925799-3 1999 When supernatants of cells stimulated with phorbol 12-myristate 13-acetate in the presence of propanil were assessed by enzyme-linked immunosorbent assay, IL-2 levels were dose-dependently decreased by 20 and 50 microM of propanil but not at 10 microM. Tetradecanoylphorbol Acetate 43-74 interleukin 2 Mus musculus 155-159 9678170-7 1998 Upon concurrent stimulation of EL4.IL-2 cells with phorbol 12-myristate-13-acetate, seven of the eight strains displayed significant enhancement of IL-2 and IL-5 production, with S. thermophilus being most effective. Tetradecanoylphorbol Acetate 51-82 interleukin 2 Mus musculus 35-39 9678170-7 1998 Upon concurrent stimulation of EL4.IL-2 cells with phorbol 12-myristate-13-acetate, seven of the eight strains displayed significant enhancement of IL-2 and IL-5 production, with S. thermophilus being most effective. Tetradecanoylphorbol Acetate 51-82 interleukin 2 Mus musculus 148-152 9406065-7 1997 Upon stimulation of EL-4.IL-2 with phorbol-12-myristate-13-acetate, there were variable increases in interleukin-2 secretion (up to 2.4-fold for 10(6) Bifidobacterium Bf-1/ml) and interleukin-5 secretion (up to 4.6-fold for 10(8) B. adolescentis M101-4). Tetradecanoylphorbol Acetate 35-66 interleukin 2 Mus musculus 25-29 9406065-7 1997 Upon stimulation of EL-4.IL-2 with phorbol-12-myristate-13-acetate, there were variable increases in interleukin-2 secretion (up to 2.4-fold for 10(6) Bifidobacterium Bf-1/ml) and interleukin-5 secretion (up to 4.6-fold for 10(8) B. adolescentis M101-4). Tetradecanoylphorbol Acetate 35-66 interleukin 2 Mus musculus 101-114 31207793-9 1997 Upon stimulation of EL4.IL-2 cells with phorbol 12-myristate-13-acetate, IL-2 secretion increased up to 1.9-fold in the presence of 106 L. bulgaricus Lr 79 cells per ml whereas this cytokine decreased in the presence of 107 or 108 lactobacilli per ml. Tetradecanoylphorbol Acetate 40-71 interleukin 2 Mus musculus 73-77 8947596-6 1996 However, the T cells of B10 mice produced high levels of IL-2 and IL-4 when stimulated by phorbol myristate acetate (PMA) and Ca2+ ionophore, proving the existence of a functionally intact signal transduction pathway downstream of protein kinase C (PKC). Tetradecanoylphorbol Acetate 117-120 interleukin 2 Mus musculus 57-61 9328180-1 1997 Phorbol ester-sensitive EL4 murine thymoma cells respond to phorbol 12-myristate 13-acetate with activation of ERK mitogen-activated protein kinases, synthesis of interleukin-2, and death, whereas phorbol ester-resistant variants of this cell line do not exhibit these responses. Tetradecanoylphorbol Acetate 60-91 interleukin 2 Mus musculus 163-176 9017603-6 1997 Translocation of mNFATx1 correlated well with activation of the murine IL-2 promoter; mNFATx1 translocated under conditions described above, in combination with phorbol 12-myristate 13-acetate, activated the transiently transfected murine IL-2 promoter. Tetradecanoylphorbol Acetate 161-192 interleukin 2 Mus musculus 71-75 9017603-6 1997 Translocation of mNFATx1 correlated well with activation of the murine IL-2 promoter; mNFATx1 translocated under conditions described above, in combination with phorbol 12-myristate 13-acetate, activated the transiently transfected murine IL-2 promoter. Tetradecanoylphorbol Acetate 161-192 interleukin 2 Mus musculus 239-243 8947596-6 1996 However, the T cells of B10 mice produced high levels of IL-2 and IL-4 when stimulated by phorbol myristate acetate (PMA) and Ca2+ ionophore, proving the existence of a functionally intact signal transduction pathway downstream of protein kinase C (PKC). Tetradecanoylphorbol Acetate 90-115 interleukin 2 Mus musculus 57-61 8943722-7 1996 However, IL-1 beta, IL-2 and TNF-alpha mRNA levels of lymph node cells from CD4- mice could be upregulated by phorbol myristate acetate in vitro. Tetradecanoylphorbol Acetate 110-135 interleukin 2 Mus musculus 20-24 8662742-6 1996 Likewise, an inhibition of phorbol 12-myristate 13-acetate (PMA)/ionomycin-induced interleukin 2 (IL-2) protein secretion, which correlated to decreased IL-2 gene transcription, was induced by both cannabinol and Delta9-THC. Tetradecanoylphorbol Acetate 27-58 interleukin 2 Mus musculus 83-96 8877730-5 1996 Murine wt p53 derived from pSG5p53cD strongly repressed the IL-2 and IL-4 promoters in both cell lines induced by the phorbol ester TPA and the Ca2+ ionophore ionomycin but not, however, in uninduced cells. Tetradecanoylphorbol Acetate 132-135 interleukin 2 Mus musculus 60-64 8662742-6 1996 Likewise, an inhibition of phorbol 12-myristate 13-acetate (PMA)/ionomycin-induced interleukin 2 (IL-2) protein secretion, which correlated to decreased IL-2 gene transcription, was induced by both cannabinol and Delta9-THC. Tetradecanoylphorbol Acetate 27-58 interleukin 2 Mus musculus 98-102 8662742-6 1996 Likewise, an inhibition of phorbol 12-myristate 13-acetate (PMA)/ionomycin-induced interleukin 2 (IL-2) protein secretion, which correlated to decreased IL-2 gene transcription, was induced by both cannabinol and Delta9-THC. Tetradecanoylphorbol Acetate 27-58 interleukin 2 Mus musculus 153-157 8662742-6 1996 Likewise, an inhibition of phorbol 12-myristate 13-acetate (PMA)/ionomycin-induced interleukin 2 (IL-2) protein secretion, which correlated to decreased IL-2 gene transcription, was induced by both cannabinol and Delta9-THC. Tetradecanoylphorbol Acetate 60-63 interleukin 2 Mus musculus 83-96 8662742-6 1996 Likewise, an inhibition of phorbol 12-myristate 13-acetate (PMA)/ionomycin-induced interleukin 2 (IL-2) protein secretion, which correlated to decreased IL-2 gene transcription, was induced by both cannabinol and Delta9-THC. Tetradecanoylphorbol Acetate 60-63 interleukin 2 Mus musculus 98-102 8662742-6 1996 Likewise, an inhibition of phorbol 12-myristate 13-acetate (PMA)/ionomycin-induced interleukin 2 (IL-2) protein secretion, which correlated to decreased IL-2 gene transcription, was induced by both cannabinol and Delta9-THC. Tetradecanoylphorbol Acetate 60-63 interleukin 2 Mus musculus 153-157 8662742-7 1996 Further, cannabinoid treatment also decreased PMA/ionomycin-induced nuclear factor binding to the AP-1 proximal site of the IL-2 promoter. Tetradecanoylphorbol Acetate 46-49 interleukin 2 Mus musculus 124-128 8144923-6 1994 dbcAMP inhibited the TPA plus ionomycin-induced transcription of IL-2 and IL-2R genes in EL4 cells, suggesting interference with biochemic events downstream to PI hydrolysis and upstream to transcription of early activation genes. Tetradecanoylphorbol Acetate 21-24 interleukin 2 Mus musculus 65-69 8543769-1 1995 Cyclic adenosine monophosphate (cAMP) inhibits phorbol 12-myristate 13-acetate (PMA)-induced IL-2 production while it inhibits IL-5 production at the transcriptional level in EL-4, a mouse lymphoma line. Tetradecanoylphorbol Acetate 47-78 interleukin 2 Mus musculus 93-97 8543769-1 1995 Cyclic adenosine monophosphate (cAMP) inhibits phorbol 12-myristate 13-acetate (PMA)-induced IL-2 production while it inhibits IL-5 production at the transcriptional level in EL-4, a mouse lymphoma line. Tetradecanoylphorbol Acetate 80-83 interleukin 2 Mus musculus 93-97 8710683-3 1996 Cell-conditioned media (CM) from EL-4 cells treated with 0.2 ng/ml phorbol myristate acetate (PMA) + 0.1 microgram/ml VES contained increased amounts of IL-2, as determined by the murine cytotoxic T cell IL-2-dependent CTLL-2 bioassay. Tetradecanoylphorbol Acetate 67-92 interleukin 2 Mus musculus 153-157 8710683-3 1996 Cell-conditioned media (CM) from EL-4 cells treated with 0.2 ng/ml phorbol myristate acetate (PMA) + 0.1 microgram/ml VES contained increased amounts of IL-2, as determined by the murine cytotoxic T cell IL-2-dependent CTLL-2 bioassay. Tetradecanoylphorbol Acetate 67-92 interleukin 2 Mus musculus 204-208 8710683-3 1996 Cell-conditioned media (CM) from EL-4 cells treated with 0.2 ng/ml phorbol myristate acetate (PMA) + 0.1 microgram/ml VES contained increased amounts of IL-2, as determined by the murine cytotoxic T cell IL-2-dependent CTLL-2 bioassay. Tetradecanoylphorbol Acetate 94-97 interleukin 2 Mus musculus 153-157 8710683-3 1996 Cell-conditioned media (CM) from EL-4 cells treated with 0.2 ng/ml phorbol myristate acetate (PMA) + 0.1 microgram/ml VES contained increased amounts of IL-2, as determined by the murine cytotoxic T cell IL-2-dependent CTLL-2 bioassay. Tetradecanoylphorbol Acetate 94-97 interleukin 2 Mus musculus 204-208 7730629-1 1995 cAMP inhibits PMA-induced IL-2 production at the transcriptional level in EL-4, a mouse lymphoma line. Tetradecanoylphorbol Acetate 14-17 interleukin 2 Mus musculus 26-30 7730629-4 1995 The IL-2 promoter carrying mutations in each element reduced response to PMA while it retained sensitivity to cAMP, thereby suggesting that multiple elements contribute to positive and negative responses to PMA and cAMP, respectively. Tetradecanoylphorbol Acetate 73-76 interleukin 2 Mus musculus 4-8 8083467-4 1994 In contrast, cAMP almost completely inhibited the PMA-dependent activation of the endogenous IL-2 gene, as well as the transfected IL-2 promoter. Tetradecanoylphorbol Acetate 50-53 interleukin 2 Mus musculus 93-97 8432620-1 1993 We investigated the role of protein kinase C activator phorbol 12-myristate 13-acetate (PMA) on IL-2-driven NK cell differentiation, by using an in vitro model previously set up by our laboratory. Tetradecanoylphorbol Acetate 55-86 interleukin 2 Mus musculus 96-100 8245456-8 1993 In contrast, Bt2cAMP almost completely inhibited the PMA-dependent activation of the endogenous IL-2 gene as well as the transfected IL-2 promoter. Tetradecanoylphorbol Acetate 53-56 interleukin 2 Mus musculus 96-100 8245456-8 1993 In contrast, Bt2cAMP almost completely inhibited the PMA-dependent activation of the endogenous IL-2 gene as well as the transfected IL-2 promoter. Tetradecanoylphorbol Acetate 53-56 interleukin 2 Mus musculus 133-137 8374508-6 1993 Vitamin E succinate plus suboptimal levels of the protein kinase C (PKC) activator phorbol myristate acetate (PMA) induced the highest levels of IL-2 by EL-4 cells. Tetradecanoylphorbol Acetate 83-108 interleukin 2 Mus musculus 145-149 8374508-6 1993 Vitamin E succinate plus suboptimal levels of the protein kinase C (PKC) activator phorbol myristate acetate (PMA) induced the highest levels of IL-2 by EL-4 cells. Tetradecanoylphorbol Acetate 110-113 interleukin 2 Mus musculus 145-149 8432620-1 1993 We investigated the role of protein kinase C activator phorbol 12-myristate 13-acetate (PMA) on IL-2-driven NK cell differentiation, by using an in vitro model previously set up by our laboratory. Tetradecanoylphorbol Acetate 88-91 interleukin 2 Mus musculus 96-100 8432620-5 1993 We now report that: (1) PMA inhibited the IL-2-induced NK cell differentiation and induced development of cells which lyse the NK-resistant target P815. Tetradecanoylphorbol Acetate 24-27 interleukin 2 Mus musculus 42-46 1558852-9 1992 Using monoclonal antibodies to neutralize IL-2 and block IL-2 receptors we showed that 1,25(OH)2D3 enhanced the IL-2-independent component of the A23187- and TPA-induced mitogenesis. Tetradecanoylphorbol Acetate 158-161 interleukin 2 Mus musculus 42-46 1382058-6 1992 Furthermore, co-stimulation of the phorbol 12-myristate 13-acetate and anti-CD3 mAb intensely provoked the transgenic thymocytes to release IL-2. Tetradecanoylphorbol Acetate 35-66 interleukin 2 Mus musculus 140-144 1331177-4 1992 In the mouse AT-20 pituitary tumor cell line, IL-2 mRNA expression was detected after stimulation with corticotropin-releasing hormone or phorbol myristate acetate. Tetradecanoylphorbol Acetate 138-163 interleukin 2 Mus musculus 46-50 1350288-6 1992 In contrast, CD4+ T cells from infected mice could be induced to proliferate by stimulation with PMA and ionomycin resulting in IL-2R up-regulation, IL-2 production, and proliferation. Tetradecanoylphorbol Acetate 97-100 interleukin 2 Mus musculus 128-132 1558852-9 1992 Using monoclonal antibodies to neutralize IL-2 and block IL-2 receptors we showed that 1,25(OH)2D3 enhanced the IL-2-independent component of the A23187- and TPA-induced mitogenesis. Tetradecanoylphorbol Acetate 158-161 interleukin 2 Mus musculus 57-61 1558852-9 1992 Using monoclonal antibodies to neutralize IL-2 and block IL-2 receptors we showed that 1,25(OH)2D3 enhanced the IL-2-independent component of the A23187- and TPA-induced mitogenesis. Tetradecanoylphorbol Acetate 158-161 interleukin 2 Mus musculus 57-61 1560050-1 1992 EL 4-6.1 cells, variants of the murine EL4 thymoma cell line, can be activated by interleukin 1 (IL-1) or phorbol 12-myristate-13-acetate (PMA), or PMA+IL-1 to secrete interleukin 2 (IL-2) and interleukin 4 (IL-4) and to express the IL-2 receptor (IL-2R). Tetradecanoylphorbol Acetate 139-142 interleukin 2 Mus musculus 168-181 1560050-1 1992 EL 4-6.1 cells, variants of the murine EL4 thymoma cell line, can be activated by interleukin 1 (IL-1) or phorbol 12-myristate-13-acetate (PMA), or PMA+IL-1 to secrete interleukin 2 (IL-2) and interleukin 4 (IL-4) and to express the IL-2 receptor (IL-2R). Tetradecanoylphorbol Acetate 139-142 interleukin 2 Mus musculus 183-187 1372331-4 1992 SP synergized with phorbol 12-myristate 13-acetate (PMA) in a dose-dependent manner to induce IL-2 production. Tetradecanoylphorbol Acetate 19-50 interleukin 2 Mus musculus 94-98 1531455-4 1992 In DBA/2 mice there was no age-related difference in IL-2 production with anti-CD3 mAb activation alone, whereas when the same cell population was activated with anti-CD3 mAb and TPA an age-associated decrease in IL-2 production occurred. Tetradecanoylphorbol Acetate 179-182 interleukin 2 Mus musculus 213-217 1372331-4 1992 SP synergized with phorbol 12-myristate 13-acetate (PMA) in a dose-dependent manner to induce IL-2 production. Tetradecanoylphorbol Acetate 52-55 interleukin 2 Mus musculus 94-98 1680700-6 1991 When concanavalin A (Con A)-activated spleen cells were restimulated with Con A and phorbol 12-myristate 13-acetate (PMA), higher levels of IL2, IL4 and IL5 mRNA were induced, as detected both by increased frequencies of positive cells, and by more mRNA per cell. Tetradecanoylphorbol Acetate 84-115 interleukin 2 Mus musculus 140-143 1655771-1 1991 We have used an interleukin-2 (IL-2) promoter-CAT fusion gene to study activation of IL-2 gene expression by IL-1, phytohemagglutinin (PHA), phorbol myristate acetate (PMA), and calcium ionophore in the murine thymoma line EL4 and the human lymphoma line Jurkat. Tetradecanoylphorbol Acetate 141-166 interleukin 2 Mus musculus 85-89 1655771-1 1991 We have used an interleukin-2 (IL-2) promoter-CAT fusion gene to study activation of IL-2 gene expression by IL-1, phytohemagglutinin (PHA), phorbol myristate acetate (PMA), and calcium ionophore in the murine thymoma line EL4 and the human lymphoma line Jurkat. Tetradecanoylphorbol Acetate 168-171 interleukin 2 Mus musculus 16-29 1655771-1 1991 We have used an interleukin-2 (IL-2) promoter-CAT fusion gene to study activation of IL-2 gene expression by IL-1, phytohemagglutinin (PHA), phorbol myristate acetate (PMA), and calcium ionophore in the murine thymoma line EL4 and the human lymphoma line Jurkat. Tetradecanoylphorbol Acetate 168-171 interleukin 2 Mus musculus 85-89 1530793-10 1992 In EL-4 cells, IL-1 potentiates PMA-mediated release of IL-2 at suboptimal concentrations of PMA. Tetradecanoylphorbol Acetate 32-35 interleukin 2 Mus musculus 56-60 1530793-10 1992 In EL-4 cells, IL-1 potentiates PMA-mediated release of IL-2 at suboptimal concentrations of PMA. Tetradecanoylphorbol Acetate 93-96 interleukin 2 Mus musculus 56-60 1530793-11 1992 Linoleic acid also augmented PMA-induced IL-2 release from the EL-4 cells. Tetradecanoylphorbol Acetate 29-32 interleukin 2 Mus musculus 41-45 1680700-6 1991 When concanavalin A (Con A)-activated spleen cells were restimulated with Con A and phorbol 12-myristate 13-acetate (PMA), higher levels of IL2, IL4 and IL5 mRNA were induced, as detected both by increased frequencies of positive cells, and by more mRNA per cell. Tetradecanoylphorbol Acetate 117-120 interleukin 2 Mus musculus 140-143 1833864-10 1991 The production of IL-2 by PMA-ionomycin stimulated T cells was not inhibited by discodermolide; however, the percentage of IL-2 receptor-bearing cells as measured by immunofluorescence with 7D4 antibody, specific for the 55-kDa chain (p55) comprising the murine IL-2 receptor, was reduced. Tetradecanoylphorbol Acetate 26-29 interleukin 2 Mus musculus 18-22 1850360-8 1991 The pattern of IL2-induced phosphorylation was different from that found following addition of phorbol 12-myristate 13-acetate (PMA) to the cells. Tetradecanoylphorbol Acetate 128-131 interleukin 2 Mus musculus 15-18 1831130-1 1991 Activation of murine spleen cells in vitro with soluble anti-CD3 monoclonal antibody and phorbol 12-myristate 13-acetate (PMA) induced an initial production of interleukin 2 (IL2), interferon-gamma (IFN-gamma), IL4 and IL5, followed by a refractory state during which the T cells did not produce lymphokines when stimulated with some common mitogens. Tetradecanoylphorbol Acetate 89-120 interleukin 2 Mus musculus 160-173 1831130-1 1991 Activation of murine spleen cells in vitro with soluble anti-CD3 monoclonal antibody and phorbol 12-myristate 13-acetate (PMA) induced an initial production of interleukin 2 (IL2), interferon-gamma (IFN-gamma), IL4 and IL5, followed by a refractory state during which the T cells did not produce lymphokines when stimulated with some common mitogens. Tetradecanoylphorbol Acetate 89-120 interleukin 2 Mus musculus 175-178 1831130-1 1991 Activation of murine spleen cells in vitro with soluble anti-CD3 monoclonal antibody and phorbol 12-myristate 13-acetate (PMA) induced an initial production of interleukin 2 (IL2), interferon-gamma (IFN-gamma), IL4 and IL5, followed by a refractory state during which the T cells did not produce lymphokines when stimulated with some common mitogens. Tetradecanoylphorbol Acetate 122-125 interleukin 2 Mus musculus 160-173 1831130-1 1991 Activation of murine spleen cells in vitro with soluble anti-CD3 monoclonal antibody and phorbol 12-myristate 13-acetate (PMA) induced an initial production of interleukin 2 (IL2), interferon-gamma (IFN-gamma), IL4 and IL5, followed by a refractory state during which the T cells did not produce lymphokines when stimulated with some common mitogens. Tetradecanoylphorbol Acetate 122-125 interleukin 2 Mus musculus 175-178 1907374-5 1991 However, the cells retained viability and functional potential because stimulation with phorbol 12-myristate 13-acetate and ionomycin bypassed the block in receptor-mediated signaling and induced IL-2 receptor expression and proliferation of the anergic cells. Tetradecanoylphorbol Acetate 88-119 interleukin 2 Mus musculus 196-200 1832383-8 1991 ED6 and LD6 mAb (in the presence of submitogenic doses of the phorbol 12-myristate 13-acetate) also induced the secretion of interleukin 2 by ED6/LD6+ T cell clones expressing TcR gamma/delta or alpha/beta. Tetradecanoylphorbol Acetate 62-93 interleukin 2 Mus musculus 125-138 1671835-1 1991 The ability of CD4+ T cells from CBA/Rij mice to produce interleukin (IL) 2 after stimulation with anti-CD3, concanavalin A, or the combination of phorbol 12-myristate 13-acetate and ionomycin declines during aging. Tetradecanoylphorbol Acetate 147-178 interleukin 2 Mus musculus 57-75 1692959-3 1990 Although IL-1 induction of the IL-2 promoter in these cells required costimulus with phorbol myristate acetate, the signal induced by IL-1 was qualitatively different. Tetradecanoylphorbol Acetate 85-110 interleukin 2 Mus musculus 31-35 1772973-2 1991 The lines used were: IM-9, a human lymphoblastoid B-cell line that spontaneously produces IgG; EL-4, a murine T-cell line that produces interleukin-2 (IL-2) on stimulation with phorbol myristate acetate; and CTLL-2, an IL-2-dependent murine T-cell line. Tetradecanoylphorbol Acetate 177-202 interleukin 2 Mus musculus 151-155 2092039-7 1990 The presence of IL-2 in the supernatants of splenocytes from GM-1/P (1mg/Kg, i.v., ,day-1) treated mice stimulated with Con A or Con A plus TPA for 48 hrs was evaluated by proliferation of an IL-2 dependent CTLL cell line. Tetradecanoylphorbol Acetate 140-143 interleukin 2 Mus musculus 16-20 2331149-3 1990 In addition, UDMH suppressed phorbol myristic acetate (PMA)-stimulated IL-2 production in EL-4 cells by up to 30% and slightly suppressed IL-2 production by Con A-stimulated murine splenocytes. Tetradecanoylphorbol Acetate 55-58 interleukin 2 Mus musculus 71-75 1971316-4 1990 However, after stimulation of the T cells with the combination of phorbol-12-myristate-13-acetate (PMA) and ionomycin to bypass CD3, 3 out of 6 old mice still exhibited 2-fold lower proliferative responses than T cells from young mice; IL-2 production by the CD4+ T cells was lower in all old mice tested. Tetradecanoylphorbol Acetate 66-97 interleukin 2 Mus musculus 236-240 1971316-4 1990 However, after stimulation of the T cells with the combination of phorbol-12-myristate-13-acetate (PMA) and ionomycin to bypass CD3, 3 out of 6 old mice still exhibited 2-fold lower proliferative responses than T cells from young mice; IL-2 production by the CD4+ T cells was lower in all old mice tested. Tetradecanoylphorbol Acetate 99-102 interleukin 2 Mus musculus 236-240 2105854-0 1990 IL-2, IL-6, and IFN-gamma have distinct effects on the IL-4 plus PMA-induced proliferation of thymocyte subpopulations. Tetradecanoylphorbol Acetate 65-68 interleukin 2 Mus musculus 0-4 2311647-4 1990 IL2 was most successfully induced with a high dose of the calcium ionophore A23187 combined with phorbol 12-myristate 13-acetate (PMA). Tetradecanoylphorbol Acetate 97-128 interleukin 2 Mus musculus 0-3 2311647-4 1990 IL2 was most successfully induced with a high dose of the calcium ionophore A23187 combined with phorbol 12-myristate 13-acetate (PMA). Tetradecanoylphorbol Acetate 130-133 interleukin 2 Mus musculus 0-3 2092039-8 1990 GM-1/P by itself was unable to stimulate IL-2 production; however it markedly increased IL-2 production induced by Con A or Con A plus TPA. Tetradecanoylphorbol Acetate 135-138 interleukin 2 Mus musculus 88-92 2492471-1 1989 Treatment of EL-4 lymphoma cells with tetradecanoylphorbol-acetate (TPA), a well-known activator of protein kinase C, induces the production of the T cell growth factor interleukin-2 (IL-2) and the expression of IL-2-specific mRNA within 4-8 h. This system is an ideal model for studies on the induction of a differentiated function in a homogeneous lymphoid cell population by a defined signal. Tetradecanoylphorbol Acetate 38-66 interleukin 2 Mus musculus 169-182 20702194-1 1990 Certain sublines of EL-4 thymoma cells can be stimulated to produce interleukin-2 (IL-2) by the mitogens phorbol-12-myristate-13-acetate and concanavalin A. EL-4 cells were cultured under conditions found to be most effective for stimulating the release of IL-2 into the culture medium. Tetradecanoylphorbol Acetate 105-136 interleukin 2 Mus musculus 68-81 20702194-1 1990 Certain sublines of EL-4 thymoma cells can be stimulated to produce interleukin-2 (IL-2) by the mitogens phorbol-12-myristate-13-acetate and concanavalin A. EL-4 cells were cultured under conditions found to be most effective for stimulating the release of IL-2 into the culture medium. Tetradecanoylphorbol Acetate 105-136 interleukin 2 Mus musculus 83-87 35068054-4 2022 Furthermore, PPP2CB deletion did not affect T cell receptor (TCR)-induced T cell activation or cytokine-induced T cell responses; however, it specifically enhanced phorbol myristate acetate (PMA) plus ionomycin-induced T cell activation with increased cellular proliferation, elevated CD69 and CD25 expression, and enhanced cytokines production (IFN-gamma, IL-2 and TNF). Tetradecanoylphorbol Acetate 164-189 interleukin 2 Mus musculus 357-361 2524528-5 1989 However, CKS-17-BSA inhibited production of IL-2 by murine thymoma cells treated with IL-1 or with 12-O-tetradecanoyl phorbol-13 acetate. Tetradecanoylphorbol Acetate 99-136 interleukin 2 Mus musculus 44-48 2522906-7 1989 Although both H-2-positive and -negative subclones expressed CD3 antigen and produced IL-2 after activation with the phorbol ester TPA, only the H-2-positive cell clones produced IL-2 and expressed IL-2 receptor after anti-CD3 antibody induction. Tetradecanoylphorbol Acetate 131-134 interleukin 2 Mus musculus 86-90 2542017-2 1989 This enhancer contains most, if not all of the sequence elements necessary for the T cell specific induction of the Il-2 gene by the phorbol ester TPA and the plant lectin Concanavalin A. Tetradecanoylphorbol Acetate 147-150 interleukin 2 Mus musculus 116-120 2565932-4 1989 Recombinant interleukin 2 (rIL-2), albeit ineffective by itself, leads to vigorous proliferation of DETC when used with either ConA or PMA + ionomycin + IL-1. Tetradecanoylphorbol Acetate 135-138 interleukin 2 Mus musculus 12-25 2492471-1 1989 Treatment of EL-4 lymphoma cells with tetradecanoylphorbol-acetate (TPA), a well-known activator of protein kinase C, induces the production of the T cell growth factor interleukin-2 (IL-2) and the expression of IL-2-specific mRNA within 4-8 h. This system is an ideal model for studies on the induction of a differentiated function in a homogeneous lymphoid cell population by a defined signal. Tetradecanoylphorbol Acetate 38-66 interleukin 2 Mus musculus 184-188 2492471-1 1989 Treatment of EL-4 lymphoma cells with tetradecanoylphorbol-acetate (TPA), a well-known activator of protein kinase C, induces the production of the T cell growth factor interleukin-2 (IL-2) and the expression of IL-2-specific mRNA within 4-8 h. This system is an ideal model for studies on the induction of a differentiated function in a homogeneous lymphoid cell population by a defined signal. Tetradecanoylphorbol Acetate 38-66 interleukin 2 Mus musculus 212-216 2492471-1 1989 Treatment of EL-4 lymphoma cells with tetradecanoylphorbol-acetate (TPA), a well-known activator of protein kinase C, induces the production of the T cell growth factor interleukin-2 (IL-2) and the expression of IL-2-specific mRNA within 4-8 h. This system is an ideal model for studies on the induction of a differentiated function in a homogeneous lymphoid cell population by a defined signal. Tetradecanoylphorbol Acetate 68-71 interleukin 2 Mus musculus 169-182 2492471-1 1989 Treatment of EL-4 lymphoma cells with tetradecanoylphorbol-acetate (TPA), a well-known activator of protein kinase C, induces the production of the T cell growth factor interleukin-2 (IL-2) and the expression of IL-2-specific mRNA within 4-8 h. This system is an ideal model for studies on the induction of a differentiated function in a homogeneous lymphoid cell population by a defined signal. Tetradecanoylphorbol Acetate 68-71 interleukin 2 Mus musculus 184-188 2492471-1 1989 Treatment of EL-4 lymphoma cells with tetradecanoylphorbol-acetate (TPA), a well-known activator of protein kinase C, induces the production of the T cell growth factor interleukin-2 (IL-2) and the expression of IL-2-specific mRNA within 4-8 h. This system is an ideal model for studies on the induction of a differentiated function in a homogeneous lymphoid cell population by a defined signal. Tetradecanoylphorbol Acetate 68-71 interleukin 2 Mus musculus 212-216 2492471-4 1989 Putrescine has rather a counter-regulatory effect as concluded from the observation that the TPA-induced TCGF production and IL-2-specific mRNA expression are augmented (superinduced) by the ODC inhibitor D,L-alpha-difluoromethylornithine (DFMO) and again suppressed after the administration of putrescine or polyamines to DFMO-treated cultures. Tetradecanoylphorbol Acetate 93-96 interleukin 2 Mus musculus 105-109 2492471-4 1989 Putrescine has rather a counter-regulatory effect as concluded from the observation that the TPA-induced TCGF production and IL-2-specific mRNA expression are augmented (superinduced) by the ODC inhibitor D,L-alpha-difluoromethylornithine (DFMO) and again suppressed after the administration of putrescine or polyamines to DFMO-treated cultures. Tetradecanoylphorbol Acetate 93-96 interleukin 2 Mus musculus 125-129 3135944-0 1988 Antioxidants inhibit proliferation and cell surface expression of receptors for interleukin-2 and transferrin in T lymphocytes stimulated with phorbol myristate acetate and ionomycin. Tetradecanoylphorbol Acetate 143-168 interleukin 2 Mus musculus 80-93 2522880-4 1989 In the presence of the tumor promoter phorbol 12-myristate 13-acetate IL 1 augments IL 2 secretion and IL 2R expression of EL4 5D3 but not of EL4 D6/76 cells. Tetradecanoylphorbol Acetate 38-69 interleukin 2 Mus musculus 84-88 2621323-1 1989 The ability of EL-4 thymoma cells to produce interleukin-2 (IL-2) following exposure to phorbol-12-myristate 13-acetate (PMA) and Concanavalin A (Con A) has been studied in vitro using medium containing either 10% or 1% fetal calf serum (FCS). Tetradecanoylphorbol Acetate 88-119 interleukin 2 Mus musculus 45-58 2621323-1 1989 The ability of EL-4 thymoma cells to produce interleukin-2 (IL-2) following exposure to phorbol-12-myristate 13-acetate (PMA) and Concanavalin A (Con A) has been studied in vitro using medium containing either 10% or 1% fetal calf serum (FCS). Tetradecanoylphorbol Acetate 88-119 interleukin 2 Mus musculus 60-64 2621323-1 1989 The ability of EL-4 thymoma cells to produce interleukin-2 (IL-2) following exposure to phorbol-12-myristate 13-acetate (PMA) and Concanavalin A (Con A) has been studied in vitro using medium containing either 10% or 1% fetal calf serum (FCS). Tetradecanoylphorbol Acetate 121-124 interleukin 2 Mus musculus 45-58 2621323-1 1989 The ability of EL-4 thymoma cells to produce interleukin-2 (IL-2) following exposure to phorbol-12-myristate 13-acetate (PMA) and Concanavalin A (Con A) has been studied in vitro using medium containing either 10% or 1% fetal calf serum (FCS). Tetradecanoylphorbol Acetate 121-124 interleukin 2 Mus musculus 60-64 3139753-1 1988 We have previously shown that Lyt-2- L3T4- T cells from the lymph nodes of MRL/lpr/lpr mice could respond to 12-O-tetradecanoylphorbol-2-acetate (TPA) and A23187 by proliferation, IL-2 secretion, and IL-2R (IL-2R) expression. Tetradecanoylphorbol Acetate 146-149 interleukin 2 Mus musculus 180-184 2439327-4 1987 To study the regulation of the murine IL2 gene in T-cell populations of differing stages of maturation, we have used a calcium ionophore in conjunction with the phorbol ester, TPA, to stimulate IL2 gene transcription while bypassing the requirement for triggering through a mature cell surface receptor. Tetradecanoylphorbol Acetate 176-179 interleukin 2 Mus musculus 194-197 3131020-0 1988 Partial restoration of Con A-induced proliferation, IL-2 receptor expression, and IL-2 synthesis in aged murine lymphocytes by phorbol myristate acetate and ionomycin. Tetradecanoylphorbol Acetate 127-152 interleukin 2 Mus musculus 82-86 3117882-1 1987 A B cell lymphoma A20.2J and splenic B cells produced an active material to support the proliferation of an interleukin 2 (IL-2)-dependent T cell line, CTLL-2, by stimulation with both calcium ionophore A23187 and phorbol myristate acetate (PMA). Tetradecanoylphorbol Acetate 214-239 interleukin 2 Mus musculus 108-121 3117882-1 1987 A B cell lymphoma A20.2J and splenic B cells produced an active material to support the proliferation of an interleukin 2 (IL-2)-dependent T cell line, CTLL-2, by stimulation with both calcium ionophore A23187 and phorbol myristate acetate (PMA). Tetradecanoylphorbol Acetate 214-239 interleukin 2 Mus musculus 123-127 3117882-1 1987 A B cell lymphoma A20.2J and splenic B cells produced an active material to support the proliferation of an interleukin 2 (IL-2)-dependent T cell line, CTLL-2, by stimulation with both calcium ionophore A23187 and phorbol myristate acetate (PMA). Tetradecanoylphorbol Acetate 241-244 interleukin 2 Mus musculus 108-121 3117882-1 1987 A B cell lymphoma A20.2J and splenic B cells produced an active material to support the proliferation of an interleukin 2 (IL-2)-dependent T cell line, CTLL-2, by stimulation with both calcium ionophore A23187 and phorbol myristate acetate (PMA). Tetradecanoylphorbol Acetate 241-244 interleukin 2 Mus musculus 123-127 3494526-1 1987 Increasing the osmolarity of the culture medium enhances the response of peanut agglutinin (PNA)-negative thymocytes to stimulation by 12-O-tetradecanoylphorbol-13-acetate (TPA), interleukin 1 (IL-1) and interleukin 2 (IL-2) in the presence of phytohemagglutinin (PHA). Tetradecanoylphorbol Acetate 173-176 interleukin 2 Mus musculus 219-223 3139787-1 1988 We have reported that human interferon-gamma (IFN-gamma) genomic DNA is expressed after transfection into a mouse T-lymphoblastoid cell line and expression can be enhanced by both interleukin 2 (IL2) and phorbol myristate acetate (PMA). Tetradecanoylphorbol Acetate 231-234 interleukin 2 Mus musculus 180-193 3139787-1 1988 We have reported that human interferon-gamma (IFN-gamma) genomic DNA is expressed after transfection into a mouse T-lymphoblastoid cell line and expression can be enhanced by both interleukin 2 (IL2) and phorbol myristate acetate (PMA). Tetradecanoylphorbol Acetate 231-234 interleukin 2 Mus musculus 195-198 3282677-1 1988 Memory lymphocytic choriomeningitis virus (LCMV)-immune cytotoxic T-lymphocyte precursors (CTLp) can be stimulated to proliferate and to mediate specific cytotoxic activity following incubation with phorbol myristate acetate (PMA), calcium ionophore (CaI), and interleukin 2 (IL-2). Tetradecanoylphorbol Acetate 199-224 interleukin 2 Mus musculus 276-280 2965729-7 1988 The presence of precursor cells for IL-2 production is supported by experiments showing that the combination of calcium ionophores and PMA elicits IL-2 production by spleen cells from both normal and T. cruzi-infected mice. Tetradecanoylphorbol Acetate 135-138 interleukin 2 Mus musculus 36-40 2965729-7 1988 The presence of precursor cells for IL-2 production is supported by experiments showing that the combination of calcium ionophores and PMA elicits IL-2 production by spleen cells from both normal and T. cruzi-infected mice. Tetradecanoylphorbol Acetate 135-138 interleukin 2 Mus musculus 147-151 3257768-10 1988 In response to PMA and ionomycin (without added IL-2), only RL-73- HSA-cells proliferated and this proliferation was correlated with IL-2 production. Tetradecanoylphorbol Acetate 15-18 interleukin 2 Mus musculus 133-137 3112932-1 1987 We investigated whether the protein kinase C-binding agents 12-O-tetradecanoyl-phorbol-13-acetate (TPA) and 1-oleoyl-2-acetyl-rac-glycerol (OAG) induced reactivity to interleukin 2 (IL-2) in mouse lymphocytes. Tetradecanoylphorbol Acetate 60-97 interleukin 2 Mus musculus 167-180 3112932-1 1987 We investigated whether the protein kinase C-binding agents 12-O-tetradecanoyl-phorbol-13-acetate (TPA) and 1-oleoyl-2-acetyl-rac-glycerol (OAG) induced reactivity to interleukin 2 (IL-2) in mouse lymphocytes. Tetradecanoylphorbol Acetate 60-97 interleukin 2 Mus musculus 182-186 3112932-1 1987 We investigated whether the protein kinase C-binding agents 12-O-tetradecanoyl-phorbol-13-acetate (TPA) and 1-oleoyl-2-acetyl-rac-glycerol (OAG) induced reactivity to interleukin 2 (IL-2) in mouse lymphocytes. Tetradecanoylphorbol Acetate 99-102 interleukin 2 Mus musculus 167-180 3112932-1 1987 We investigated whether the protein kinase C-binding agents 12-O-tetradecanoyl-phorbol-13-acetate (TPA) and 1-oleoyl-2-acetyl-rac-glycerol (OAG) induced reactivity to interleukin 2 (IL-2) in mouse lymphocytes. Tetradecanoylphorbol Acetate 99-102 interleukin 2 Mus musculus 182-186 3112932-2 1987 10(-8) M TPA was a strong inducer of reactivity to IL-2 measured by [3H]thymidine incorporation. Tetradecanoylphorbol Acetate 9-12 interleukin 2 Mus musculus 51-55 3112932-6 1987 The expression of reactivity to IL-2 induced by A 23187 + OAG was inhibited by 0.04 mM Mn2+; in contrast the TPA-mediated induction of IL-2 responsiveness was not affected by Mn2+. Tetradecanoylphorbol Acetate 109-112 interleukin 2 Mus musculus 135-139 3112932-7 1987 The data suggest differences in the mechanism of induction of IL-2 responsiveness by the two protein kinase C-binding agents, TPA and OAG. Tetradecanoylphorbol Acetate 126-129 interleukin 2 Mus musculus 62-66 3496224-3 1987 TPA stimulation of an interleukin 2 (IL 2)-producing variant, EL4+, induced a 3-4-fold increase in TCR beta, but not alpha, chain mRNA. Tetradecanoylphorbol Acetate 0-3 interleukin 2 Mus musculus 22-35 3496224-3 1987 TPA stimulation of an interleukin 2 (IL 2)-producing variant, EL4+, induced a 3-4-fold increase in TCR beta, but not alpha, chain mRNA. Tetradecanoylphorbol Acetate 0-3 interleukin 2 Mus musculus 37-41 3496224-6 1987 TPA stimulation of EL4+ cells also induced de novo expression of the IL 2 gene and subsequent secretion of this lymphokine. Tetradecanoylphorbol Acetate 0-3 interleukin 2 Mus musculus 69-73 3496224-7 1987 However, the increased expression of the TCR beta-chain gene and the induction of the IL 2 gene were not linked since expression of TCR beta-chain mRNA was increased to a similar degree in EL4+ and IL 2-nonproducing EL4- sublines, and cyclosporin A selectively blocked TPA-induced IL 2-gene expression in EL4+ cells without affecting the increase in TCR beta-chain mRNA. Tetradecanoylphorbol Acetate 269-272 interleukin 2 Mus musculus 86-90 3104323-3 1987 A combination of a phorbol ester, 12-O-tetradecanoylphorbol-13-acetate (TPA), and a calcium ionophore, A23187, induced the expression of IL2 receptors on resting 6-1 cells and induced DNA synthesis in the presence of IL2. Tetradecanoylphorbol Acetate 34-70 interleukin 2 Mus musculus 137-140 3104323-3 1987 A combination of a phorbol ester, 12-O-tetradecanoylphorbol-13-acetate (TPA), and a calcium ionophore, A23187, induced the expression of IL2 receptors on resting 6-1 cells and induced DNA synthesis in the presence of IL2. Tetradecanoylphorbol Acetate 34-70 interleukin 2 Mus musculus 217-220 3104323-3 1987 A combination of a phorbol ester, 12-O-tetradecanoylphorbol-13-acetate (TPA), and a calcium ionophore, A23187, induced the expression of IL2 receptors on resting 6-1 cells and induced DNA synthesis in the presence of IL2. Tetradecanoylphorbol Acetate 72-75 interleukin 2 Mus musculus 137-140 3104323-3 1987 A combination of a phorbol ester, 12-O-tetradecanoylphorbol-13-acetate (TPA), and a calcium ionophore, A23187, induced the expression of IL2 receptors on resting 6-1 cells and induced DNA synthesis in the presence of IL2. Tetradecanoylphorbol Acetate 72-75 interleukin 2 Mus musculus 217-220 3104323-7 1987 Resting KO.6 cells produced IL2 in response to a combination of TPA and A23187, whereas either one of the agents did not induce the production of IL2. Tetradecanoylphorbol Acetate 64-67 interleukin 2 Mus musculus 28-31 3104323-10 1987 On the contrary, signals for DNA synthesis generated by binding of IL2 to IL2 receptors are different from those for IL2 production and IL2 receptor expression, as the combination of TPA and A23187 could not induce DNA synthesis without IL2. Tetradecanoylphorbol Acetate 183-186 interleukin 2 Mus musculus 67-70 3104323-10 1987 On the contrary, signals for DNA synthesis generated by binding of IL2 to IL2 receptors are different from those for IL2 production and IL2 receptor expression, as the combination of TPA and A23187 could not induce DNA synthesis without IL2. Tetradecanoylphorbol Acetate 183-186 interleukin 2 Mus musculus 74-77 3104323-10 1987 On the contrary, signals for DNA synthesis generated by binding of IL2 to IL2 receptors are different from those for IL2 production and IL2 receptor expression, as the combination of TPA and A23187 could not induce DNA synthesis without IL2. Tetradecanoylphorbol Acetate 183-186 interleukin 2 Mus musculus 74-77 3104323-10 1987 On the contrary, signals for DNA synthesis generated by binding of IL2 to IL2 receptors are different from those for IL2 production and IL2 receptor expression, as the combination of TPA and A23187 could not induce DNA synthesis without IL2. Tetradecanoylphorbol Acetate 183-186 interleukin 2 Mus musculus 74-77 3104323-10 1987 On the contrary, signals for DNA synthesis generated by binding of IL2 to IL2 receptors are different from those for IL2 production and IL2 receptor expression, as the combination of TPA and A23187 could not induce DNA synthesis without IL2. Tetradecanoylphorbol Acetate 183-186 interleukin 2 Mus musculus 74-77 3108164-0 1987 Interleukin-2 responses of MRL/lpr mouse splenocytes and lymph node cells induced by TPA and A23187. Tetradecanoylphorbol Acetate 85-88 interleukin 2 Mus musculus 0-13 3104061-5 1987 Either accessory cells, RIF or the protein kinase C activator phorbol myristate acetate can substitute for each other and are equally active for the induction of IL 2 responsiveness in high-density Lyt-2+ T cells exposed to Con A. Tetradecanoylphorbol Acetate 62-87 interleukin 2 Mus musculus 162-166 3108164-1 1987 Treatment of splenocytes and lymph node cells of 5 month-old MRL/lpr mice with TPA induced IL-2-dependent proliferation of the cells in the presence of CA++. Tetradecanoylphorbol Acetate 79-82 interleukin 2 Mus musculus 91-95 3108164-3 1987 The combination of TPA and A23187 in the lpr cells induced both proliferation and production of IL-2 in a Ca++-dependent fashion. Tetradecanoylphorbol Acetate 19-22 interleukin 2 Mus musculus 96-100 3102248-10 1986 Second, with regard to the requirements for receptor expression, it was observed that either anti-Ig or phorbol diester (phorbol-12-myristate 13-acetate) can induce IL 2R and IL 2 responsiveness (proliferation assay) in LPS blasts but not in fresh B cells. Tetradecanoylphorbol Acetate 121-152 interleukin 2 Mus musculus 165-169 3097128-11 1986 PMA alone induced an IL 2-independent proliferative response. Tetradecanoylphorbol Acetate 0-3 interleukin 2 Mus musculus 21-25 3100060-5 1986 TPA strongly synergized with ionomycin both for Il-2 secretion and for Il-2 receptor expression whereas Il-1 did not. Tetradecanoylphorbol Acetate 0-3 interleukin 2 Mus musculus 48-52 3100060-5 1986 TPA strongly synergized with ionomycin both for Il-2 secretion and for Il-2 receptor expression whereas Il-1 did not. Tetradecanoylphorbol Acetate 0-3 interleukin 2 Mus musculus 71-75 3100060-7 1986 However, on EL4 cells which were also partially responsive to TPA alone, Il-1 showed strong synergy with TPA to induce Il-2 secretion and Il-2 receptor expression. Tetradecanoylphorbol Acetate 105-108 interleukin 2 Mus musculus 119-123 3100060-7 1986 However, on EL4 cells which were also partially responsive to TPA alone, Il-1 showed strong synergy with TPA to induce Il-2 secretion and Il-2 receptor expression. Tetradecanoylphorbol Acetate 105-108 interleukin 2 Mus musculus 138-142 3100060-8 1986 The effect of Il-1 on EL4 cells was dose dependent where increasingly higher concentrations of Il-1 in the presence of a fixed concentration of TPA caused higher percentage of EL4 cells to become Il-2 receptor positive. Tetradecanoylphorbol Acetate 144-147 interleukin 2 Mus musculus 196-200 3093371-2 1986 Induction of IL-2 receptor (IL-2R), IL-2 production and subsequent de novo DNA synthesis in MRL/l mice by the tumour-promoting phorbol ester 12-o-tetradecanoyl phorbol 13-acetate (TPA) and calcium ionophore (A23187) were examined. Tetradecanoylphorbol Acetate 141-178 interleukin 2 Mus musculus 13-17 2945862-4 1986 In a second assay, rIL 1 enhanced proliferation and IL 2 production by 2-4- cells in response to phorbol myristate acetate (PMA) and the calcium ionophore, ionomycin. Tetradecanoylphorbol Acetate 97-122 interleukin 2 Mus musculus 52-56 2945862-4 1986 In a second assay, rIL 1 enhanced proliferation and IL 2 production by 2-4- cells in response to phorbol myristate acetate (PMA) and the calcium ionophore, ionomycin. Tetradecanoylphorbol Acetate 124-127 interleukin 2 Mus musculus 52-56 2944961-6 1986 The proliferation of HTL in response to PMA + A23187 could be completely inhibited either by cyclosporine A (CsA) or by PC61.5, a monoclonal antibody directed against the murine IL 2 receptor; however, the proliferation of CTL in response to PMA alone was not affected either by CsA or by PC61.5. Tetradecanoylphorbol Acetate 40-43 interleukin 2 Mus musculus 178-182 2944961-6 1986 The proliferation of HTL in response to PMA + A23187 could be completely inhibited either by cyclosporine A (CsA) or by PC61.5, a monoclonal antibody directed against the murine IL 2 receptor; however, the proliferation of CTL in response to PMA alone was not affected either by CsA or by PC61.5. Tetradecanoylphorbol Acetate 242-245 interleukin 2 Mus musculus 178-182 3492276-0 1986 Phorbol myristate acetate-induced proliferation of an IL-2-dependent T-cell line: action of PMA is independent of IL-2 and cannot be mimicked by diacylglycerols. Tetradecanoylphorbol Acetate 0-25 interleukin 2 Mus musculus 54-58 3492276-0 1986 Phorbol myristate acetate-induced proliferation of an IL-2-dependent T-cell line: action of PMA is independent of IL-2 and cannot be mimicked by diacylglycerols. Tetradecanoylphorbol Acetate 0-25 interleukin 2 Mus musculus 114-118 3492276-1 1986 Phorbol myristate acetate (PMA) at concentrations of 2 X 10(-10) to 2 X 10(-7) M was able to sustain proliferation of the interleukin 2 (IL-2)-dependent murine T-cell line CTLL-K. Tetradecanoylphorbol Acetate 0-25 interleukin 2 Mus musculus 122-135 3492276-1 1986 Phorbol myristate acetate (PMA) at concentrations of 2 X 10(-10) to 2 X 10(-7) M was able to sustain proliferation of the interleukin 2 (IL-2)-dependent murine T-cell line CTLL-K. Tetradecanoylphorbol Acetate 0-25 interleukin 2 Mus musculus 137-141 3492276-1 1986 Phorbol myristate acetate (PMA) at concentrations of 2 X 10(-10) to 2 X 10(-7) M was able to sustain proliferation of the interleukin 2 (IL-2)-dependent murine T-cell line CTLL-K. Tetradecanoylphorbol Acetate 27-30 interleukin 2 Mus musculus 122-135 3492276-1 1986 Phorbol myristate acetate (PMA) at concentrations of 2 X 10(-10) to 2 X 10(-7) M was able to sustain proliferation of the interleukin 2 (IL-2)-dependent murine T-cell line CTLL-K. Tetradecanoylphorbol Acetate 27-30 interleukin 2 Mus musculus 137-141 3093570-5 1986 Interestingly, the in vitro growth of PMA + ionomycin-stimulated fetal thymocytes appeared to be IL 2 dependent in that it was inhibited by a monoclonal antibody to the IL 2 receptor. Tetradecanoylphorbol Acetate 38-41 interleukin 2 Mus musculus 97-101 3093570-5 1986 Interestingly, the in vitro growth of PMA + ionomycin-stimulated fetal thymocytes appeared to be IL 2 dependent in that it was inhibited by a monoclonal antibody to the IL 2 receptor. Tetradecanoylphorbol Acetate 38-41 interleukin 2 Mus musculus 169-173 3096619-11 1986 In addition, the data suggest distinct, but co-operative pathways of IL-2 receptor induction, controlled by elevated Ca2+ alone and by protein kinase C. Subsequent intracellular events of T cell activation by A23187/PMA may be quite similar to those triggered by Con A, since both kinds of stimulation are blocked by agents such as cyclosporin A, dbcAMP and K+ channel blockers. Tetradecanoylphorbol Acetate 216-219 interleukin 2 Mus musculus 69-73 3090144-5 1986 In the presence of either suboptimal levels of phorbol ester (PMA) or Ionomycin, the addition of IL 1 resulted in up to an 80-fold enhancement in the amount of IL 2 secreted. Tetradecanoylphorbol Acetate 62-65 interleukin 2 Mus musculus 160-164 3082993-1 1986 The murine T lymphoma EL4 subline produces large amounts of interleukin 2 (IL-2) upon stimulation with phorbol myristate acetate (PMA). Tetradecanoylphorbol Acetate 103-128 interleukin 2 Mus musculus 60-73 3011244-2 1986 A crude supernatant from phorbol myristate acetate exposed EL-4 cells rich in IL-2 plus other lymphokines (EL-4 IL-2), a concanavalin A-induced supernatant from murine splenocytes (Con A IL-2), and recombinant IL-2 (rIL-2) provided by Biogen were tested. Tetradecanoylphorbol Acetate 25-50 interleukin 2 Mus musculus 78-82 3011244-2 1986 A crude supernatant from phorbol myristate acetate exposed EL-4 cells rich in IL-2 plus other lymphokines (EL-4 IL-2), a concanavalin A-induced supernatant from murine splenocytes (Con A IL-2), and recombinant IL-2 (rIL-2) provided by Biogen were tested. Tetradecanoylphorbol Acetate 25-50 interleukin 2 Mus musculus 112-116 3011244-2 1986 A crude supernatant from phorbol myristate acetate exposed EL-4 cells rich in IL-2 plus other lymphokines (EL-4 IL-2), a concanavalin A-induced supernatant from murine splenocytes (Con A IL-2), and recombinant IL-2 (rIL-2) provided by Biogen were tested. Tetradecanoylphorbol Acetate 25-50 interleukin 2 Mus musculus 112-116 3011244-2 1986 A crude supernatant from phorbol myristate acetate exposed EL-4 cells rich in IL-2 plus other lymphokines (EL-4 IL-2), a concanavalin A-induced supernatant from murine splenocytes (Con A IL-2), and recombinant IL-2 (rIL-2) provided by Biogen were tested. Tetradecanoylphorbol Acetate 25-50 interleukin 2 Mus musculus 112-116 3082993-1 1986 The murine T lymphoma EL4 subline produces large amounts of interleukin 2 (IL-2) upon stimulation with phorbol myristate acetate (PMA). Tetradecanoylphorbol Acetate 103-128 interleukin 2 Mus musculus 75-79 3082993-1 1986 The murine T lymphoma EL4 subline produces large amounts of interleukin 2 (IL-2) upon stimulation with phorbol myristate acetate (PMA). Tetradecanoylphorbol Acetate 130-133 interleukin 2 Mus musculus 60-73 3082993-1 1986 The murine T lymphoma EL4 subline produces large amounts of interleukin 2 (IL-2) upon stimulation with phorbol myristate acetate (PMA). Tetradecanoylphorbol Acetate 130-133 interleukin 2 Mus musculus 75-79 3082993-6 1986 The reverted EL4 cells were again able to produce large amounts of IL-2 upon restimulation with PMA. Tetradecanoylphorbol Acetate 96-99 interleukin 2 Mus musculus 67-71 3082993-9 1986 PMA appears to act at the level of the expression of the IL-2 gene, since IL-2 mRNA was much more abundant in the IL-2-secreting PMA-treated cells than in untreated controls. Tetradecanoylphorbol Acetate 0-3 interleukin 2 Mus musculus 57-61 3082993-9 1986 PMA appears to act at the level of the expression of the IL-2 gene, since IL-2 mRNA was much more abundant in the IL-2-secreting PMA-treated cells than in untreated controls. Tetradecanoylphorbol Acetate 0-3 interleukin 2 Mus musculus 74-78 3082993-9 1986 PMA appears to act at the level of the expression of the IL-2 gene, since IL-2 mRNA was much more abundant in the IL-2-secreting PMA-treated cells than in untreated controls. Tetradecanoylphorbol Acetate 0-3 interleukin 2 Mus musculus 74-78 2427445-1 1986 The thymic leukemia cell line EL4 has been shown to produce the lymphokine Interleukin-2 (IL-2) following stimulation with phorbol ester (PMA). Tetradecanoylphorbol Acetate 138-141 interleukin 2 Mus musculus 75-88 2427445-1 1986 The thymic leukemia cell line EL4 has been shown to produce the lymphokine Interleukin-2 (IL-2) following stimulation with phorbol ester (PMA). Tetradecanoylphorbol Acetate 138-141 interleukin 2 Mus musculus 90-94 3081905-4 1986 We found that these "double-negative" thymocytes were unresponsive to Con A plus PMA but produced substantial amounts of IL-2 when stimulated with A23187 plus PMA. Tetradecanoylphorbol Acetate 159-162 interleukin 2 Mus musculus 121-125 3005278-4 1986 The effect of IL 2 on CT6 cell proliferation and cAMP production was mimicked by the phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA), which, like IL 2, causes a translocation and activation of protein kinase C. While PGE2 stimulated adenylate cyclase activity in membrane preparations, neither IL 2 nor TPA inhibited either basal or stimulated membrane adenylate cyclase activity. Tetradecanoylphorbol Acetate 137-140 interleukin 2 Mus musculus 14-18 3005278-4 1986 The effect of IL 2 on CT6 cell proliferation and cAMP production was mimicked by the phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA), which, like IL 2, causes a translocation and activation of protein kinase C. While PGE2 stimulated adenylate cyclase activity in membrane preparations, neither IL 2 nor TPA inhibited either basal or stimulated membrane adenylate cyclase activity. Tetradecanoylphorbol Acetate 137-140 interleukin 2 Mus musculus 155-159 3005278-4 1986 The effect of IL 2 on CT6 cell proliferation and cAMP production was mimicked by the phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA), which, like IL 2, causes a translocation and activation of protein kinase C. While PGE2 stimulated adenylate cyclase activity in membrane preparations, neither IL 2 nor TPA inhibited either basal or stimulated membrane adenylate cyclase activity. Tetradecanoylphorbol Acetate 137-140 interleukin 2 Mus musculus 155-159 3005278-4 1986 The effect of IL 2 on CT6 cell proliferation and cAMP production was mimicked by the phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA), which, like IL 2, causes a translocation and activation of protein kinase C. While PGE2 stimulated adenylate cyclase activity in membrane preparations, neither IL 2 nor TPA inhibited either basal or stimulated membrane adenylate cyclase activity. Tetradecanoylphorbol Acetate 312-315 interleukin 2 Mus musculus 14-18 3005278-4 1986 The effect of IL 2 on CT6 cell proliferation and cAMP production was mimicked by the phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA), which, like IL 2, causes a translocation and activation of protein kinase C. While PGE2 stimulated adenylate cyclase activity in membrane preparations, neither IL 2 nor TPA inhibited either basal or stimulated membrane adenylate cyclase activity. Tetradecanoylphorbol Acetate 312-315 interleukin 2 Mus musculus 155-159 3005278-4 1986 The effect of IL 2 on CT6 cell proliferation and cAMP production was mimicked by the phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA), which, like IL 2, causes a translocation and activation of protein kinase C. While PGE2 stimulated adenylate cyclase activity in membrane preparations, neither IL 2 nor TPA inhibited either basal or stimulated membrane adenylate cyclase activity. Tetradecanoylphorbol Acetate 312-315 interleukin 2 Mus musculus 155-159 3918270-6 1985 However, either one in combination with the phorbol ester 12-O-tetradecanoylphorbol 13-acetate (TPA), which is structurally related to 1,2-diacylglycerol, induces in lymphoid cell populations the expression of receptors for interleukin-2 (IL-2), the secretion of IL-2 and cell proliferation as measured by 3H-thymidine uptake. Tetradecanoylphorbol Acetate 58-94 interleukin 2 Mus musculus 224-237 3081352-2 1986 The combination of phorbol myristate acetate (PMA), ionomycin and recombinant interleukin 2 (IL2) is both sufficient and necessary to induce growth of about 1/6.2 (range 1/3-1/9) and 1/4.3 (range 1/2-1/7) immature thymocytes from adult and fetal mice, respectively, in serum-free cultures. Tetradecanoylphorbol Acetate 19-44 interleukin 2 Mus musculus 93-96 2999231-4 1985 Both calcium ionophores, A23187 and ionomycin, in conjunction with 12-O-tetradecanoylphorbol 13-acetate (TPA) mimicked the effect of antigen or interleukin 2 (IL 2) by inducing strong proliferative and alloantigen-specific cytotoxic responses. Tetradecanoylphorbol Acetate 67-103 interleukin 2 Mus musculus 144-157 2999231-4 1985 Both calcium ionophores, A23187 and ionomycin, in conjunction with 12-O-tetradecanoylphorbol 13-acetate (TPA) mimicked the effect of antigen or interleukin 2 (IL 2) by inducing strong proliferative and alloantigen-specific cytotoxic responses. Tetradecanoylphorbol Acetate 67-103 interleukin 2 Mus musculus 159-163 2999231-4 1985 Both calcium ionophores, A23187 and ionomycin, in conjunction with 12-O-tetradecanoylphorbol 13-acetate (TPA) mimicked the effect of antigen or interleukin 2 (IL 2) by inducing strong proliferative and alloantigen-specific cytotoxic responses. Tetradecanoylphorbol Acetate 105-108 interleukin 2 Mus musculus 144-157 2999231-4 1985 Both calcium ionophores, A23187 and ionomycin, in conjunction with 12-O-tetradecanoylphorbol 13-acetate (TPA) mimicked the effect of antigen or interleukin 2 (IL 2) by inducing strong proliferative and alloantigen-specific cytotoxic responses. Tetradecanoylphorbol Acetate 105-108 interleukin 2 Mus musculus 159-163 2999231-5 1985 In addition, Ca2+ ionophore and TPA induced IL 2 receptor expression and IL 2 secretion. Tetradecanoylphorbol Acetate 32-35 interleukin 2 Mus musculus 44-48 2999231-5 1985 In addition, Ca2+ ionophore and TPA induced IL 2 receptor expression and IL 2 secretion. Tetradecanoylphorbol Acetate 32-35 interleukin 2 Mus musculus 73-77 3932189-5 1985 The cells rendered IL-2-responsive by PMA are enriched in the more mature peanut agglutinin (PNA)-negative population. Tetradecanoylphorbol Acetate 38-41 interleukin 2 Mus musculus 19-23 3918270-6 1985 However, either one in combination with the phorbol ester 12-O-tetradecanoylphorbol 13-acetate (TPA), which is structurally related to 1,2-diacylglycerol, induces in lymphoid cell populations the expression of receptors for interleukin-2 (IL-2), the secretion of IL-2 and cell proliferation as measured by 3H-thymidine uptake. Tetradecanoylphorbol Acetate 58-94 interleukin 2 Mus musculus 239-243 3918270-6 1985 However, either one in combination with the phorbol ester 12-O-tetradecanoylphorbol 13-acetate (TPA), which is structurally related to 1,2-diacylglycerol, induces in lymphoid cell populations the expression of receptors for interleukin-2 (IL-2), the secretion of IL-2 and cell proliferation as measured by 3H-thymidine uptake. Tetradecanoylphorbol Acetate 58-94 interleukin 2 Mus musculus 263-267 3918270-6 1985 However, either one in combination with the phorbol ester 12-O-tetradecanoylphorbol 13-acetate (TPA), which is structurally related to 1,2-diacylglycerol, induces in lymphoid cell populations the expression of receptors for interleukin-2 (IL-2), the secretion of IL-2 and cell proliferation as measured by 3H-thymidine uptake. Tetradecanoylphorbol Acetate 96-99 interleukin 2 Mus musculus 224-237 3918270-6 1985 However, either one in combination with the phorbol ester 12-O-tetradecanoylphorbol 13-acetate (TPA), which is structurally related to 1,2-diacylglycerol, induces in lymphoid cell populations the expression of receptors for interleukin-2 (IL-2), the secretion of IL-2 and cell proliferation as measured by 3H-thymidine uptake. Tetradecanoylphorbol Acetate 96-99 interleukin 2 Mus musculus 239-243 3918270-6 1985 However, either one in combination with the phorbol ester 12-O-tetradecanoylphorbol 13-acetate (TPA), which is structurally related to 1,2-diacylglycerol, induces in lymphoid cell populations the expression of receptors for interleukin-2 (IL-2), the secretion of IL-2 and cell proliferation as measured by 3H-thymidine uptake. Tetradecanoylphorbol Acetate 96-99 interleukin 2 Mus musculus 263-267 6441115-10 1984 Murine thymocytes incubated with PMA for 30 min or with ConA for 4 hr (mitogen-pulsed T-cells) failed to bind the anti-IL-2 receptor antibody AMT-13 and to absorb IL-2 activity present in semipurified IL-2 preparations, but they proliferated vigorously in response to the same IL-2 preparations. Tetradecanoylphorbol Acetate 33-36 interleukin 2 Mus musculus 119-123 6441115-10 1984 Murine thymocytes incubated with PMA for 30 min or with ConA for 4 hr (mitogen-pulsed T-cells) failed to bind the anti-IL-2 receptor antibody AMT-13 and to absorb IL-2 activity present in semipurified IL-2 preparations, but they proliferated vigorously in response to the same IL-2 preparations. Tetradecanoylphorbol Acetate 33-36 interleukin 2 Mus musculus 163-167 6441115-10 1984 Murine thymocytes incubated with PMA for 30 min or with ConA for 4 hr (mitogen-pulsed T-cells) failed to bind the anti-IL-2 receptor antibody AMT-13 and to absorb IL-2 activity present in semipurified IL-2 preparations, but they proliferated vigorously in response to the same IL-2 preparations. Tetradecanoylphorbol Acetate 33-36 interleukin 2 Mus musculus 163-167 6610701-8 1984 The IL 2 defect could be significantly repaired by the addition of PMA to the cultures. Tetradecanoylphorbol Acetate 67-70 interleukin 2 Mus musculus 4-8 6334241-0 1984 Phorbol myristate acetate-stimulated production of interleukin 2 by T-cell lymphoma and constitutive production by derived hybridomas. Tetradecanoylphorbol Acetate 0-25 interleukin 2 Mus musculus 51-64 6232000-5 1984 In the presence of PHA and phorbol myristate acetate, FL cells produced a growth factor, tentatively identified as Interleukin 2 (IL-2) by its ability to promote the proliferation of an IL-2-dependent murine T-cell line. Tetradecanoylphorbol Acetate 27-52 interleukin 2 Mus musculus 115-128 6232000-5 1984 In the presence of PHA and phorbol myristate acetate, FL cells produced a growth factor, tentatively identified as Interleukin 2 (IL-2) by its ability to promote the proliferation of an IL-2-dependent murine T-cell line. Tetradecanoylphorbol Acetate 27-52 interleukin 2 Mus musculus 130-134 6232000-5 1984 In the presence of PHA and phorbol myristate acetate, FL cells produced a growth factor, tentatively identified as Interleukin 2 (IL-2) by its ability to promote the proliferation of an IL-2-dependent murine T-cell line. Tetradecanoylphorbol Acetate 27-52 interleukin 2 Mus musculus 186-190 6086044-8 1984 While spleen cells from such mice were, indeed, unable to produce IL 2 or to proliferate when stimulated with concanavalin A (Con A), the combination of Con A plus the comitogen phorbol myristate acetate (PMA) engendered substantial IL 2 production and normal cellular proliferation. Tetradecanoylphorbol Acetate 178-203 interleukin 2 Mus musculus 233-237 6086044-10 1984 We found that culturing lpr spleen cells with an anti-IL 2 receptor antibody abrogated the mitogenicity of Con A + PMA; that on stimulation with Con A + PMA, MRL-lpr/lpr T cells expressed IL 2 receptors, and that addition of recombinant IL 2 to the receptor positive population resulted in marked proliferation. Tetradecanoylphorbol Acetate 115-118 interleukin 2 Mus musculus 54-58 6086044-10 1984 We found that culturing lpr spleen cells with an anti-IL 2 receptor antibody abrogated the mitogenicity of Con A + PMA; that on stimulation with Con A + PMA, MRL-lpr/lpr T cells expressed IL 2 receptors, and that addition of recombinant IL 2 to the receptor positive population resulted in marked proliferation. Tetradecanoylphorbol Acetate 115-118 interleukin 2 Mus musculus 188-192 6086044-10 1984 We found that culturing lpr spleen cells with an anti-IL 2 receptor antibody abrogated the mitogenicity of Con A + PMA; that on stimulation with Con A + PMA, MRL-lpr/lpr T cells expressed IL 2 receptors, and that addition of recombinant IL 2 to the receptor positive population resulted in marked proliferation. Tetradecanoylphorbol Acetate 115-118 interleukin 2 Mus musculus 188-192 6425405-1 1984 To obtain an anti-interleukin 2 (IL 2) receptor antibody, we immunized rats with phorbol myristate acetate-pulsed mouse T lymphoblasts. Tetradecanoylphorbol Acetate 81-106 interleukin 2 Mus musculus 33-37 6335128-0 1984 Interleukin 2-dependent T cell line acquires responsiveness to phorbol myristate acetate and lipopolysaccharide in the course of long-term culture. Tetradecanoylphorbol Acetate 63-88 interleukin 2 Mus musculus 0-13 6335128-1 1984 We have observed that CT6 cell line, a murine interleukin 2 (IL 2)-dependent T cell line, was highly responsive to phorbol myristate acetate (PMA) and to a lesser extent but significantly responsive to lipopolysaccharide (LPS) in the short-term proliferation assay. Tetradecanoylphorbol Acetate 115-140 interleukin 2 Mus musculus 46-59 6335128-1 1984 We have observed that CT6 cell line, a murine interleukin 2 (IL 2)-dependent T cell line, was highly responsive to phorbol myristate acetate (PMA) and to a lesser extent but significantly responsive to lipopolysaccharide (LPS) in the short-term proliferation assay. Tetradecanoylphorbol Acetate 115-140 interleukin 2 Mus musculus 61-65 6335128-1 1984 We have observed that CT6 cell line, a murine interleukin 2 (IL 2)-dependent T cell line, was highly responsive to phorbol myristate acetate (PMA) and to a lesser extent but significantly responsive to lipopolysaccharide (LPS) in the short-term proliferation assay. Tetradecanoylphorbol Acetate 142-145 interleukin 2 Mus musculus 46-59 6335128-1 1984 We have observed that CT6 cell line, a murine interleukin 2 (IL 2)-dependent T cell line, was highly responsive to phorbol myristate acetate (PMA) and to a lesser extent but significantly responsive to lipopolysaccharide (LPS) in the short-term proliferation assay. Tetradecanoylphorbol Acetate 142-145 interleukin 2 Mus musculus 61-65 6228583-4 1984 The surviving spleen cells were then cultured for another 5 days under the same conditions plus 10% interleukin 2-rich supernatant from a clone of EL-4 thymoma cells stimulated with phorbol-12-myristate-13-acetate. Tetradecanoylphorbol Acetate 182-213 interleukin 2 Mus musculus 100-113 6604573-6 1983 TPA induces the production of interleukin-2 (IL-2) in lectin-treated PNA-negative populations but not in PNA-positive cells. Tetradecanoylphorbol Acetate 0-3 interleukin 2 Mus musculus 30-43 6226737-2 1983 Supernatant from phorbol myristate acetate-induced EL-4 cells was used as a source of IL 2 in these assays, which therefore were independent of the presence of the Lyt-2-, IL 2-producing cells known to be deficient in aging mice. Tetradecanoylphorbol Acetate 17-42 interleukin 2 Mus musculus 86-90 6604573-6 1983 TPA induces the production of interleukin-2 (IL-2) in lectin-treated PNA-negative populations but not in PNA-positive cells. Tetradecanoylphorbol Acetate 0-3 interleukin 2 Mus musculus 45-49 6408170-12 1983 Our results indicate that TPA mimics IL 1 in the induction of differentiation of the ST to a stage in which subpopulations of these cells are able to produce IL 2 and to respond to PHA. Tetradecanoylphorbol Acetate 26-29 interleukin 2 Mus musculus 158-162 6603367-1 1983 The effect of concanavalin A (Con A) and/or phorbol myristate acetate (PMA) on interleukin 2 (IL 2) production and tritiated thymidine incorporation was measured in young (6 weeks) and old (16-24 weeks) autoimmune mice by pulsing 5 X 10(6) unfractionated spleen cells with 5 micrograms of Con A and/or 5 ng of PMA for variable periods of time. Tetradecanoylphorbol Acetate 44-69 interleukin 2 Mus musculus 79-92 6603367-1 1983 The effect of concanavalin A (Con A) and/or phorbol myristate acetate (PMA) on interleukin 2 (IL 2) production and tritiated thymidine incorporation was measured in young (6 weeks) and old (16-24 weeks) autoimmune mice by pulsing 5 X 10(6) unfractionated spleen cells with 5 micrograms of Con A and/or 5 ng of PMA for variable periods of time. Tetradecanoylphorbol Acetate 71-74 interleukin 2 Mus musculus 79-92 6603367-1 1983 The effect of concanavalin A (Con A) and/or phorbol myristate acetate (PMA) on interleukin 2 (IL 2) production and tritiated thymidine incorporation was measured in young (6 weeks) and old (16-24 weeks) autoimmune mice by pulsing 5 X 10(6) unfractionated spleen cells with 5 micrograms of Con A and/or 5 ng of PMA for variable periods of time. Tetradecanoylphorbol Acetate 71-74 interleukin 2 Mus musculus 94-98 6411958-3 1983 Costimulation with 500 ng 12-O-tetradecanoylphorbol 13-acetate (TPA)/ml and 5 micrograms concanavalin A (Con A)/ml induced optimal levels of IL-2. Tetradecanoylphorbol Acetate 26-62 interleukin 2 Mus musculus 141-145 6411958-3 1983 Costimulation with 500 ng 12-O-tetradecanoylphorbol 13-acetate (TPA)/ml and 5 micrograms concanavalin A (Con A)/ml induced optimal levels of IL-2. Tetradecanoylphorbol Acetate 64-67 interleukin 2 Mus musculus 141-145 6411958-5 1983 Kinetic experiments indicated that IL-2 first became detectable at 2 hours in TPA-treated cultures, whereas in cultures stimulated with Con A alone IL-2 production was not evident until 8 hours. Tetradecanoylphorbol Acetate 78-81 interleukin 2 Mus musculus 35-39 6411958-6 1983 Flow cytometry indicated that TPA and its related phorbol esters cause a perturbation in the cycling of the cell which may be related to increased IL-2 production. Tetradecanoylphorbol Acetate 30-33 interleukin 2 Mus musculus 147-151 6813857-1 1982 An interleukin 2-independent murine T cell line (BFS) was isolated that produced immune interferon after stimulation with phorbol 12-myristate 13-acetate. Tetradecanoylphorbol Acetate 122-153 interleukin 2 Mus musculus 3-16 6401187-1 1983 In order to obtain an anti-interleukin 2 (IL 2) receptor antibody, we immunized mice with phorbol myristate acetate-pulsed rat T lymphoblasts. Tetradecanoylphorbol Acetate 90-115 interleukin 2 Mus musculus 27-40 6401187-1 1983 In order to obtain an anti-interleukin 2 (IL 2) receptor antibody, we immunized mice with phorbol myristate acetate-pulsed rat T lymphoblasts. Tetradecanoylphorbol Acetate 90-115 interleukin 2 Mus musculus 42-46 6982822-1 1982 Splenocytes of nu/nu mice treated with serum thymic factor (FTS) for 3 or more days followed by stimulation with either phorbol myristate acetate (PMA) or concanavalin A (Con A) produced interleukin 2 (IL 2) as determined in two indicator systems, namely, support of growth of IL 2-dependent T cells and promotion of Con A-initiated mitogenesis of thymocytes. Tetradecanoylphorbol Acetate 120-145 interleukin 2 Mus musculus 187-200 6982822-1 1982 Splenocytes of nu/nu mice treated with serum thymic factor (FTS) for 3 or more days followed by stimulation with either phorbol myristate acetate (PMA) or concanavalin A (Con A) produced interleukin 2 (IL 2) as determined in two indicator systems, namely, support of growth of IL 2-dependent T cells and promotion of Con A-initiated mitogenesis of thymocytes. Tetradecanoylphorbol Acetate 120-145 interleukin 2 Mus musculus 202-206 6982822-1 1982 Splenocytes of nu/nu mice treated with serum thymic factor (FTS) for 3 or more days followed by stimulation with either phorbol myristate acetate (PMA) or concanavalin A (Con A) produced interleukin 2 (IL 2) as determined in two indicator systems, namely, support of growth of IL 2-dependent T cells and promotion of Con A-initiated mitogenesis of thymocytes. Tetradecanoylphorbol Acetate 147-150 interleukin 2 Mus musculus 187-200 6982822-1 1982 Splenocytes of nu/nu mice treated with serum thymic factor (FTS) for 3 or more days followed by stimulation with either phorbol myristate acetate (PMA) or concanavalin A (Con A) produced interleukin 2 (IL 2) as determined in two indicator systems, namely, support of growth of IL 2-dependent T cells and promotion of Con A-initiated mitogenesis of thymocytes. Tetradecanoylphorbol Acetate 147-150 interleukin 2 Mus musculus 202-206 6982822-1 1982 Splenocytes of nu/nu mice treated with serum thymic factor (FTS) for 3 or more days followed by stimulation with either phorbol myristate acetate (PMA) or concanavalin A (Con A) produced interleukin 2 (IL 2) as determined in two indicator systems, namely, support of growth of IL 2-dependent T cells and promotion of Con A-initiated mitogenesis of thymocytes. Tetradecanoylphorbol Acetate 147-150 interleukin 2 Mus musculus 277-281 30815315-4 2019 Compounds 5, 7, and 9 significantly increased IL-2 secretion in phorbol 12-myristate 13-acetate (PMA)/ionomycin (Io)-induced EL-4 T cells. Tetradecanoylphorbol Acetate 64-95 interleukin 2 Mus musculus 46-50 6975322-1 1981 A variant line of murine T lymphoma EL4 produces high levels of the lymphokine Interleukin 2 (IL 2) when it is stimulated with phorbol myristate acetate. Tetradecanoylphorbol Acetate 127-152 interleukin 2 Mus musculus 79-92 6975322-1 1981 A variant line of murine T lymphoma EL4 produces high levels of the lymphokine Interleukin 2 (IL 2) when it is stimulated with phorbol myristate acetate. Tetradecanoylphorbol Acetate 127-152 interleukin 2 Mus musculus 94-98 6975322-6 1981 The IL 2 produced by injected oocytes from a given preparation of mRNA is about 1% of the amount produced by the EL4 cells stimulated originally with phorbol myristate acetate. Tetradecanoylphorbol Acetate 150-175 interleukin 2 Mus musculus 4-8 6975322-8 1981 We conclude that IL 2 is essentially protein in nature, that the protein is coded for by poly A+ mRNA, and that the amount of this mRNA increases significantly after stimulation of the variant EL4 cells with the inducer phorbol myristate acetate. Tetradecanoylphorbol Acetate 220-245 interleukin 2 Mus musculus 17-21 6170682-1 1981 Two growth factors, interleukin 2 (T cell growth factor) and colony-stimulating factor, are produced concomitantly by a murine EL-4 thymoma cell line after stimulation by phorbol myristate acetate. Tetradecanoylphorbol Acetate 171-196 interleukin 2 Mus musculus 20-33 6170682-1 1981 Two growth factors, interleukin 2 (T cell growth factor) and colony-stimulating factor, are produced concomitantly by a murine EL-4 thymoma cell line after stimulation by phorbol myristate acetate. Tetradecanoylphorbol Acetate 171-196 interleukin 2 Mus musculus 35-56 6968789-1 1980 Phorbol-12-myristate-13-acetate stimulates a subline of mouse EL-4 thymoma cells to produce, in vitro, in very high titer, T cell growth factor (Interleukin 2, IL 2). Tetradecanoylphorbol Acetate 0-31 interleukin 2 Mus musculus 123-143 6968789-1 1980 Phorbol-12-myristate-13-acetate stimulates a subline of mouse EL-4 thymoma cells to produce, in vitro, in very high titer, T cell growth factor (Interleukin 2, IL 2). Tetradecanoylphorbol Acetate 0-31 interleukin 2 Mus musculus 145-158 6968789-1 1980 Phorbol-12-myristate-13-acetate stimulates a subline of mouse EL-4 thymoma cells to produce, in vitro, in very high titer, T cell growth factor (Interleukin 2, IL 2). Tetradecanoylphorbol Acetate 0-31 interleukin 2 Mus musculus 160-164 6968790-7 1980 Peak IL-2 production by LBRM-33 cultures (stimulated at either optimal Con A or PHA concentrations or co-stimulated with suboptimal amounts of mitogen and phorbol myristate acetate) was consistently accompanied by LBRM-33 cell death. Tetradecanoylphorbol Acetate 155-180 interleukin 2 Mus musculus 5-9 30721636-9 2019 Both Con A and PMA + Ion-induced secretion of IL-2, IFN-gamma, and TNF-alpha were attenuated by PHI. Tetradecanoylphorbol Acetate 15-18 interleukin 2 Mus musculus 46-50 33162030-7 2021 Interleukin (IL)-2 production in cultured LPL upon stimulation with phorbol 12-myristate 13-acetate and ionomycin for 24 h was significantly lower in SPF mice than in GF mice. Tetradecanoylphorbol Acetate 68-99 interleukin 2 Mus musculus 0-18 30815315-4 2019 Compounds 5, 7, and 9 significantly increased IL-2 secretion in phorbol 12-myristate 13-acetate (PMA)/ionomycin (Io)-induced EL-4 T cells. Tetradecanoylphorbol Acetate 97-100 interleukin 2 Mus musculus 46-50 26319521-5 2015 At the same time, the results showed that infected mouse splenic NK cells expressed increased levels of CD25 and CD69 and produced more IL-2, IL-4, and IL-17 and less IFN-gamma after stimulation with PMA and ionomycin. Tetradecanoylphorbol Acetate 200-203 interleukin 2 Mus musculus 136-140 29433394-8 2018 The W-EA fraction significantly increased IL-2 secretion in EL-4 T cells activated with phorbol 12-myristate 13-acetate (PMA) and ionomycin (Io). Tetradecanoylphorbol Acetate 88-119 interleukin 2 Mus musculus 42-46 29433394-8 2018 The W-EA fraction significantly increased IL-2 secretion in EL-4 T cells activated with phorbol 12-myristate 13-acetate (PMA) and ionomycin (Io). Tetradecanoylphorbol Acetate 121-124 interleukin 2 Mus musculus 42-46 20728597-7 2010 Interestingly, maximal IL-2 production and CD4(+) T cell cycle progression are observed upon activation with soluble anti-CD3 and phorbol 12-myristate 13-acetate (PMA), a phorbol ester. Tetradecanoylphorbol Acetate 130-161 interleukin 2 Mus musculus 23-27 20728597-7 2010 Interestingly, maximal IL-2 production and CD4(+) T cell cycle progression are observed upon activation with soluble anti-CD3 and phorbol 12-myristate 13-acetate (PMA), a phorbol ester. Tetradecanoylphorbol Acetate 163-166 interleukin 2 Mus musculus 23-27