PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 24764294-0 2014 Endothelial nitric-oxide synthase (eNOS) is activated through G-protein-coupled receptor kinase-interacting protein 1 (GIT1) tyrosine phosphorylation and Src protein. Tyrosine 125-133 nitric oxide synthase 3 Homo sapiens 0-33 24764294-0 2014 Endothelial nitric-oxide synthase (eNOS) is activated through G-protein-coupled receptor kinase-interacting protein 1 (GIT1) tyrosine phosphorylation and Src protein. Tyrosine 125-133 nitric oxide synthase 3 Homo sapiens 35-39 24764294-6 2014 GIT1 was phosphorylated on tyrosine by Src, and Y293F and Y554F mutations reduced GIT1 phosphorylation as well as the ability of GIT1 to bind to and activate eNOS. Tyrosine 27-35 nitric oxide synthase 3 Homo sapiens 158-162 18483407-2 2008 We found that fluid shear stress induces the association of eNOS with the proline-rich tyrosine kinase 2 (PYK2) in endothelial cells and that the eNOS immunoprecipitated from eNOS- and PYK2-overexpressing HEK293 cells was tyrosine-phosphorylated on Tyr657. Tyrosine 87-95 nitric oxide synthase 3 Homo sapiens 60-64 23727078-0 2013 Calcineurin-mediated dephosphorylation of eNOS at serine 116 affects eNOS enzymatic activity indirectly by facilitating c-Src binding and tyrosine 83 phosphorylation. Tyrosine 138-146 nitric oxide synthase 3 Homo sapiens 42-46 22323292-7 2012 Finally, Src FRET biosensor, eNOS small interfering RNA, and NO donor studies demonstrate NO-induced Src activation and Cav-1 phosphorylation at Tyr-14, resulting in increased eNOS/Cav-1 interaction and inhibition of eNOS activity. Tyrosine 145-148 nitric oxide synthase 3 Homo sapiens 176-180 22323292-7 2012 Finally, Src FRET biosensor, eNOS small interfering RNA, and NO donor studies demonstrate NO-induced Src activation and Cav-1 phosphorylation at Tyr-14, resulting in increased eNOS/Cav-1 interaction and inhibition of eNOS activity. Tyrosine 145-148 nitric oxide synthase 3 Homo sapiens 176-180 22323292-8 2012 Taken together, these data suggest that activation of eNOS promotes Src-dependent Cav-1-Tyr-14 phosphorylation and eNOS/Cav-1 binding, that is, eNOS feedback inhibition. Tyrosine 88-91 nitric oxide synthase 3 Homo sapiens 54-58 23727078-5 2013 Phospho-mimicking, however, does interfere with the interaction of eNOS with c-Src, an interaction which is known to activate eNOS by phosphorylation at tyrosine 83 (Y83). Tyrosine 153-161 nitric oxide synthase 3 Homo sapiens 67-71 23727078-5 2013 Phospho-mimicking, however, does interfere with the interaction of eNOS with c-Src, an interaction which is known to activate eNOS by phosphorylation at tyrosine 83 (Y83). Tyrosine 153-161 nitric oxide synthase 3 Homo sapiens 126-130 20363286-5 2010 The phosphorylation state of specific serine, threonine and tyrosine residues of the enzyme plays a pivotal role in regulation of eNOS activity. Tyrosine 60-68 nitric oxide synthase 3 Homo sapiens 130-134 15665327-0 2005 Flow shear stress stimulates Gab1 tyrosine phosphorylation to mediate protein kinase B and endothelial nitric-oxide synthase activation in endothelial cells. Tyrosine 34-42 nitric oxide synthase 3 Homo sapiens 91-124 17409706-3 2007 In vascular endothelial cells, microsphere embolism-induced eNOS expression was associated with protein tyrosine nitration, which is a marker of generation of peroxynitrite. Tyrosine 104-112 nitric oxide synthase 3 Homo sapiens 60-64 16118242-6 2005 While the shear-stress-induced tyrosine phosphorylation of PECAM-1 as well as the serine phosphorylation of Akt and eNOS were abolished by the pre-treatment of cells with the tyrosine kinase inhibitor PP1 the phosphorylation of AMPK was unaffected. Tyrosine 31-39 nitric oxide synthase 3 Homo sapiens 116-120 16118242-9 2005 These data indicate that the shear-stress-induced activation of Akt and eNOS in endothelial cells is modulated by the tyrosine phosphorylation of PECAM-1 whereas the shear-stress-induced phosphorylation of AMPK is controlled by an alternative signaling pathway. Tyrosine 118-126 nitric oxide synthase 3 Homo sapiens 72-76 16898954-1 2006 BACKGROUND: Endothelial nitric oxide synthase (eNOS) activity in endothelial cells is regulated by post-translational phosphorylation of critical serine, threonine and tyrosine residues in response to a variety of stimuli. Tyrosine 168-176 nitric oxide synthase 3 Homo sapiens 12-45 16898954-1 2006 BACKGROUND: Endothelial nitric oxide synthase (eNOS) activity in endothelial cells is regulated by post-translational phosphorylation of critical serine, threonine and tyrosine residues in response to a variety of stimuli. Tyrosine 168-176 nitric oxide synthase 3 Homo sapiens 47-51 16898954-3 2006 OBJECTIVES: We investigated the role of tyrosine phosphorylation in the regulation of platelet eNOS activity. Tyrosine 40-48 nitric oxide synthase 3 Homo sapiens 95-99 16898954-4 2006 METHODS: Tyrosine phosphorylation of eNOS and interaction with the tyrosine phosphatase SHP-1 were investigated by coimmunoprecipitation and immunoblotting. Tyrosine 9-17 nitric oxide synthase 3 Homo sapiens 37-41 16898954-6 2006 RESULTS: We found platelet eNOS was tyrosine phosphorylated under basal conditions. Tyrosine 36-44 nitric oxide synthase 3 Homo sapiens 27-31 16898954-8 2006 In vitro tyrosine dephosphorylation of platelet eNOS using a recombinant protein tyrosine phosphatase enhanced thrombin-induced activity compared to thrombin alone, but had no effect on endothelial eNOS activity either at basal or after stimulation with bradykinin. Tyrosine 9-17 nitric oxide synthase 3 Homo sapiens 48-52 16898954-12 2006 CONCLUSIONS: Our data suggest a novel role for tyrosine dephosphorylation in platelet eNOS activation, which may be mediated by SHP-1. Tyrosine 47-55 nitric oxide synthase 3 Homo sapiens 86-90 15817516-5 2005 Here we show that E2-mediated activation of endothelial nitric oxide synthase (eNOS) increases the production of nitric oxide (NO), which, under Abeta(E22Q)-induced oxidative damage, results in the formation of peroxynitrite and increased nitration of tyrosine residues. Tyrosine 252-260 nitric oxide synthase 3 Homo sapiens 44-77 15817516-5 2005 Here we show that E2-mediated activation of endothelial nitric oxide synthase (eNOS) increases the production of nitric oxide (NO), which, under Abeta(E22Q)-induced oxidative damage, results in the formation of peroxynitrite and increased nitration of tyrosine residues. Tyrosine 252-260 nitric oxide synthase 3 Homo sapiens 79-83 12590926-0 2003 Differential effects of mutations in human endothelial nitric oxide synthase at residues Tyr-357 and Arg-365 on L-arginine hydroxylation and GN-hydroxy-L-arginine oxidation. Tyrosine 89-92 nitric oxide synthase 3 Homo sapiens 43-76 15069547-0 2004 Tyrosine phosphorylation of NOS3 in a breast cancer cell line and Src-transformed cells. Tyrosine 0-8 nitric oxide synthase 3 Homo sapiens 28-32 15069547-1 2004 We investigated the tyrosine phosphorylation of NOS3 by active Src. Tyrosine 20-28 nitric oxide synthase 3 Homo sapiens 48-52 15069547-2 2004 In a cell line derived from human breast cancer, BT474, we found activation of c-Src and tyrosine phosphorylation of NOS3. Tyrosine 89-97 nitric oxide synthase 3 Homo sapiens 117-121 12874836-7 2003 Heating also increased cNOs phosphorylation at tyrosine residues. Tyrosine 47-55 nitric oxide synthase 3 Homo sapiens 23-27 15740982-4 2005 In these cells, eNOS activity is modulated by tyrosine- and serine-phosphorylation. Tyrosine 46-54 nitric oxide synthase 3 Homo sapiens 16-20 15740982-7 2005 Therefore, the present results indicate that the molecular mechanism of clinically observed anti-inflammatory action of ESW should include tyrosine-dephosphorylation of eNOS, a successive increase in NO production and suppression of NF-kappaB activation. Tyrosine 139-147 nitric oxide synthase 3 Homo sapiens 169-173 31479717-11 2019 Combining Tyr capsule(s) with WW abolished the increase in iNOS, eNOS, VEGFA, and CHF expressions promoted by WW (p < 0.05). Tyrosine 10-13 nitric oxide synthase 3 Homo sapiens 65-69 9852077-1 1998 Eight polar amino acid residues in the putative substrate-binding region from Thr-360 to Val-379 in human endothelial nitric-oxide synthase (eNOS) (Thr-360, Arg-365, Cys-368, Asp-369, Arg-372, Tyr-373, Glu-377, and Asp-378) were individually mutated. Tyrosine 193-196 nitric oxide synthase 3 Homo sapiens 106-139 9226298-6 1997 A pharmacologically identical activation of NOS III can be induced by protein phosphatase inhibitors suggesting that the tyrosine phosphorylation of NOS III or an associated regulatory protein is crucial for its Ca2(+)-independent activation. Tyrosine 121-129 nitric oxide synthase 3 Homo sapiens 44-51 9226298-6 1997 A pharmacologically identical activation of NOS III can be induced by protein phosphatase inhibitors suggesting that the tyrosine phosphorylation of NOS III or an associated regulatory protein is crucial for its Ca2(+)-independent activation. Tyrosine 121-129 nitric oxide synthase 3 Homo sapiens 149-156 32653904-8 2021 Two kinases i.e. abelson-tyrosine protein kinase (ABL)1 and Src were identified as eNOS Tyr81 kinases as their inhibition and downregulation significantly reduced the basal and Yoda1-induced tyrosine phosphorylation and activity of eNOS. Tyrosine 25-33 nitric oxide synthase 3 Homo sapiens 83-87 32653904-8 2021 Two kinases i.e. abelson-tyrosine protein kinase (ABL)1 and Src were identified as eNOS Tyr81 kinases as their inhibition and downregulation significantly reduced the basal and Yoda1-induced tyrosine phosphorylation and activity of eNOS. Tyrosine 25-33 nitric oxide synthase 3 Homo sapiens 232-236 11384194-3 2001 Our experiments clearly showed that human fibroblasts with and without peroxisomal deficiencies only contain the constitutively expressed endothelial nitric oxide synthase (eNOS) isoform and that the eNOS is tyrosine-phosphorylated. Tyrosine 208-216 nitric oxide synthase 3 Homo sapiens 200-204 11384194-6 2001 However the tyrosine-phosphorylated portion of eNOS is significantly lower in X-ALD and Zellweger cells. Tyrosine 12-20 nitric oxide synthase 3 Homo sapiens 47-51 10978247-6 2000 Western blots of immunoprecipitated eNOS showed the presence of a major tyrosine-phosphorylated protein band at a mass corresponding to approximately 125 kDa and 2 minor bands corresponding to approximately 105 and 75 kDa after treatment with vanadate/Bk. Tyrosine 72-80 nitric oxide synthase 3 Homo sapiens 36-40 10978247-9 2000 These results show that there is a clear link between tyrosine phosphorylation and stimulation of eNO production, which does not appear to involve direct modification of eNOS, changes in eNOS levels, or compartmentation, but rather appears to be due to changes in proteins associating with eNOS, thereby enhancing the state of activation of eNOS. Tyrosine 54-62 nitric oxide synthase 3 Homo sapiens 187-191 10978247-9 2000 These results show that there is a clear link between tyrosine phosphorylation and stimulation of eNO production, which does not appear to involve direct modification of eNOS, changes in eNOS levels, or compartmentation, but rather appears to be due to changes in proteins associating with eNOS, thereby enhancing the state of activation of eNOS. Tyrosine 54-62 nitric oxide synthase 3 Homo sapiens 187-191 10978247-9 2000 These results show that there is a clear link between tyrosine phosphorylation and stimulation of eNO production, which does not appear to involve direct modification of eNOS, changes in eNOS levels, or compartmentation, but rather appears to be due to changes in proteins associating with eNOS, thereby enhancing the state of activation of eNOS. Tyrosine 54-62 nitric oxide synthase 3 Homo sapiens 187-191 9837744-5 1998 Moreover, the astroglial NOS-III-like protein is constitutively tyrosine-phosphorylated and associated with caveolin-1. Tyrosine 64-72 nitric oxide synthase 3 Homo sapiens 25-32 35195045-2 2022 In cultured ECs tyrosine phosphorylation of PECAM-1 has been shown to activate eNOS in response to shear stress. Tyrosine 16-24 nitric oxide synthase 3 Homo sapiens 79-83