PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 9740224-2 1998 Nickel is the most common contact sensitizer in humans and nickel-specific CD4+ T helper cells have been extensively characterized. Nickel 59-65 CD4 molecule Homo sapiens 75-78 8566016-4 1995 They were shown to recognize nickel in the context of major histocompatibility complex (MHC) class II molecules and to belong to the CD4 subset. Nickel 29-35 CD4 molecule Homo sapiens 133-136 9714417-0 1998 Restricted and individual usage of T-cell receptor beta-gene variables in nickel-induced CD4+ and CD8+ cells. Nickel 74-80 CD4 molecule Homo sapiens 89-92 9714417-2 1998 We examined the T-cell receptor (TCR) beta-chain variable gene segment (Vbeta) use of nickel-induced CD4+ and CD8+ T cells in the peripheral blood of nickel-sensitive and nonsensitized subjects. Nickel 86-92 CD4 molecule Homo sapiens 101-104 9714417-7 1998 In conclusion, an individual pattern of restricted Vbeta genes was induced with nickel in CD4+ and CD8+ T cells in each nickel allergy patient. Nickel 80-86 CD4 molecule Homo sapiens 90-93 9714417-7 1998 In conclusion, an individual pattern of restricted Vbeta genes was induced with nickel in CD4+ and CD8+ T cells in each nickel allergy patient. Nickel 120-126 CD4 molecule Homo sapiens 90-93 2976621-0 1988 Nickel antigen induces IL-2 secretion and IL-2 receptor expression mainly on CD4+ T cells, but no measurable gamma interferon secretion in peripheral blood mononuclear cell cultures in delayed type hypersensitivity to nickel. Nickel 0-6 CD4 molecule Homo sapiens 77-80 1345823-0 1992 Th1 lymphokine production profiles of nickel-specific CD4+T-lymphocyte clones from nickel contact allergic and non-allergic individuals. Nickel 38-44 CD4 molecule Homo sapiens 54-57 1345823-0 1992 Th1 lymphokine production profiles of nickel-specific CD4+T-lymphocyte clones from nickel contact allergic and non-allergic individuals. Nickel 83-89 CD4 molecule Homo sapiens 54-57 31249573-0 2019 Identification and Characterization of Circulating Naive CD4+ and CD8+ T Cells Recognizing Nickel. Nickel 91-97 CD4 molecule Homo sapiens 57-60 30779159-6 2019 RESULTS: Patch test positivity for nickel, PPD and MI was associated with changes in the phenotype of peripheral blood T cells: increases in naive cells, decreases in regulatory T cell frequency and the CD4+ /CD8hi ratio, and increased expression of the skin-homing marker cutaneous lymphocyte antigen (CLA), particularly for those patients with a +++ patch test score. Nickel 35-41 CD4 molecule Homo sapiens 203-206 32298488-0 2020 TCRs with segment TRAV9-2 or a CDR3 histidine are overrepresented among nickel-specific CD4+ T cells. Nickel 72-78 CD4 molecule Homo sapiens 88-91 32298488-2 2020 In vitro, CD4+ T cells from all donors respond to nickel but the involved alphabeta T cell receptor (TCR) repertoire has not been comprehensively analyzed. Nickel 50-56 CD4 molecule Homo sapiens 10-13 32298488-3 2020 METHODS: We introduce CD154 (CD40L) upregulation as a fast, unbiased, and quantitative method to detect nickel-specific CD4+ T cells ex vivo in blood of clinically characterized allergic and non-allergic donors. Nickel 104-110 CD4 molecule Homo sapiens 29-32 32298488-8 2020 Among nickel-specific CD4+ T cells of allergic and non-allergic donors, TCRs expressing the alpha-chain segment TRAV9-2 or a histidine in their alpha- or beta-chain complementarity determining region 3 (CDR3) were highly overrepresented. Nickel 6-12 CD4 molecule Homo sapiens 22-25 32298488-9 2020 CONCLUSIONS: Induced CD154 expression represents a reliable method to study nickel-specific CD4+ T cells. Nickel 76-82 CD4 molecule Homo sapiens 92-95 31249573-4 2019 The purpose of this study was to identify and quantify naive CD4+ and CD8+ T cells recognizing nickel in the general population. Nickel 95-101 CD4 molecule Homo sapiens 61-64 31249573-5 2019 Using a T-cell priming in vitro assay based on autologous co-cultures between naive T cells and dendritic cells loaded with nickel, we were able to detect a naive CD4+ and CD8+ T-cell repertoire for nickel in 10/11 and 7/8 of the tested donors. Nickel 124-130 CD4 molecule Homo sapiens 163-166 31249573-5 2019 Using a T-cell priming in vitro assay based on autologous co-cultures between naive T cells and dendritic cells loaded with nickel, we were able to detect a naive CD4+ and CD8+ T-cell repertoire for nickel in 10/11 and 7/8 of the tested donors. Nickel 199-205 CD4 molecule Homo sapiens 163-166 31249573-6 2019 We calculated a mean frequency of 0.49 nickel-specific naive CD4+ T cells and 0.37 nickel-specific naive CD8+ T cells per million of circulating naive T cells. Nickel 39-45 CD4 molecule Homo sapiens 61-64 31249573-8 2019 Interestingly, nickel-specific naive CD4+ and CD8+ T cells showed a low rate of cross-reactivity with cobalt, another metallic hapten, frequently mixed with nickel in many alloys. Nickel 15-21 CD4 molecule Homo sapiens 37-40 31249573-8 2019 Interestingly, nickel-specific naive CD4+ and CD8+ T cells showed a low rate of cross-reactivity with cobalt, another metallic hapten, frequently mixed with nickel in many alloys. Nickel 157-163 CD4 molecule Homo sapiens 37-40 23692034-0 2013 CD4(+) T cells producing interleukin (IL)-17, IL-22 and interferon-gamma are major effector T cells in nickel allergy. Nickel 103-109 CD4 molecule Homo sapiens 0-3 30356071-0 2018 Enrichment and Quantification of Epitope-specific CD4+ T Lymphocytes using Ferromagnetic Iron-gold and Nickel Nanowires. Nickel 103-109 CD4 molecule Homo sapiens 50-53 23692034-6 2013 In nickel-allergic patients, there was massive cellular infiltration dominated by CD4(+) T cells producing IL-17, IL-22 and IFN-gamma in nickel-challenged skin but not in vehicle-challenged skin. Nickel 3-9 CD4 molecule Homo sapiens 82-85 23692034-7 2013 CONCLUSION: CD4(+) T cells producing IL-17, IL-22 and IFN-gamma are important effector cells in the eczematous reactions of nickel-induced allergic contact dermatitis in humans. Nickel 124-130 CD4 molecule Homo sapiens 12-15 31148973-8 2019 Exposure to nickel induced expression of CD69 on a significantly higher proportion of CD4+ lymphocytes (22.1+-6.2%) of the ACD patients compared to controls (2.8+-2.5%;p<0.0001). Nickel 12-18 CD4 molecule Homo sapiens 86-89 30109436-8 2018 RESULTS: Nickel-sensitive patients showed a statistical increase in CD4+ reactivity compared to CD8+ reactivity. Nickel 9-15 CD4 molecule Homo sapiens 68-71 30109436-9 2018 The ratio of CD4+/CD8+ T lymphocytes was 1.28 in nickel-sensitive patients versus 0.76 in the control (p = 0.009). Nickel 49-55 CD4 molecule Homo sapiens 13-16 25601470-4 2015 Nickel-induced oral tolerance is another immune tolerance model that is induced by the contact allergen Nickel and leads to the generation of Nickel-specific CD4(+) CD25(+) T regulatory cells after oral exposure. Nickel 0-6 CD4 molecule Homo sapiens 158-161 25601470-4 2015 Nickel-induced oral tolerance is another immune tolerance model that is induced by the contact allergen Nickel and leads to the generation of Nickel-specific CD4(+) CD25(+) T regulatory cells after oral exposure. Nickel 104-110 CD4 molecule Homo sapiens 158-161 25601470-4 2015 Nickel-induced oral tolerance is another immune tolerance model that is induced by the contact allergen Nickel and leads to the generation of Nickel-specific CD4(+) CD25(+) T regulatory cells after oral exposure. Nickel 104-110 CD4 molecule Homo sapiens 158-161 16033398-4 2005 These regulatory T cells were anergic to nickel but inhibited nickel-specific CD4+CD25- effector T cells in coculture experiments. Nickel 62-68 CD4 molecule Homo sapiens 78-81 17263646-2 2007 The exposure of affected individuals to nickel leads to a delayed-type hypersensitivity reaction, which is induced by antigen-specific CD4 and CD8 T cells. Nickel 40-46 CD4 molecule Homo sapiens 135-138 17263646-7 2007 Data obtained with nickel-specific CD4 T cell clones showed that antigen-mediated proliferation is an absolute prerequisite for HIV expansion. Nickel 19-25 CD4 molecule Homo sapiens 35-38 20696642-3 2010 To investigate the prediction ability of a flow cytometric assay of CD69 up-regulation on CD4+ CLA+ T cells in nickel-sensitive and non-nickel-sensitive patients. Nickel 111-117 CD4 molecule Homo sapiens 90-93 16033398-0 2005 Regulation of nickel-induced T-cell responsiveness by CD4+CD25+ cells in contact allergic patients and healthy individuals. Nickel 14-20 CD4 molecule Homo sapiens 54-57 16033398-1 2005 In this study, we investigated the capacity of CD4+CD25+ regulatory T cells to suppress nickel-specific effector T cells, both in nickel-allergic patients and healthy controls. Nickel 88-94 CD4 molecule Homo sapiens 47-50 16033398-2 2005 CD4+ cells isolated from allergic patients showed an increased proliferative response to nickel, whereas CD4+ cells from negative controls did not respond to allergen. Nickel 89-95 CD4 molecule Homo sapiens 0-3 16033398-3 2005 When CD4+CD25+ cells were depleted, nickel-specific responsiveness was strongly increased both in allergic and in non-allergic individuals, with the most pronounced effect in allergic patients. Nickel 36-42 CD4 molecule Homo sapiens 5-8 16033398-5 2005 CD4+CD25+ cells from nickel-allergic patients showed only a limited capacity to suppress effector T-cell responsiveness, because an increased nickel reactivity could still be detected in these cocultures. Nickel 21-27 CD4 molecule Homo sapiens 0-3 16033398-5 2005 CD4+CD25+ cells from nickel-allergic patients showed only a limited capacity to suppress effector T-cell responsiveness, because an increased nickel reactivity could still be detected in these cocultures. Nickel 142-148 CD4 molecule Homo sapiens 0-3 16033398-7 2005 Overall, these results support the view that CD4+CD25+ cells can control the activation of nickel-specific effector T cells in non-allergic individuals, whereas this regulatory capacity is impaired in allergic patients. Nickel 91-97 CD4 molecule Homo sapiens 45-48 14634084-2 2003 CD4(+) T cells isolated from the peripheral blood of six healthy, nonallergic individuals showed a limited capacity to proliferate in response to nickel in vitro, but responsiveness was strongly augmented (mean increment +/- SD, 240 +/- 60%) when cells were depleted of CD25(+) Treg. Nickel 146-152 CD4 molecule Homo sapiens 0-3 15767023-4 2005 Among these, CD4(+)CD25+ T cells from peripheral blood of non allergic subjects strongly regulate immune responses to nickel in a cytokine-independent, cell-contact-dependent mechanism. Nickel 118-124 CD4 molecule Homo sapiens 13-16 15767023-5 2005 In contrast, CD4(+)CD25+ obtained from the blood of nickel-allergic individuals have limited or absent suppressive activity on specific T cell responses in vitro. Nickel 52-58 CD4 molecule Homo sapiens 13-16 15270870-0 2004 Nickel-responding T cells are CD4+ CLA+ CD45RO+ and express chemokine receptors CXCR3, CCR4 and CCR10. Nickel 0-6 CD4 molecule Homo sapiens 30-33 15270870-5 2004 RESULTS: Only CD4+ CLA+ CD45RO+ and not CD8+ T cells proliferate and produce both type-1 (IFN-gamma) and type-2 (IL-5) cytokines in response to nickel. Nickel 144-150 CD4 molecule Homo sapiens 14-17 15270870-6 2004 Moreover, cells expressing the marker CLA in combination with CD4, CD45RO or CD69 are increased after nickel-specific stimulation. Nickel 102-108 CD4 molecule Homo sapiens 62-65 14634084-6 2003 The site of a negative patch test in response to nickel showed an infiltrate of CD4(+)CLA(+) cells and CD25(+) cells, which accounted for approximately 20% of the total T cells isolated from the tissue. Nickel 49-55 CD4 molecule Homo sapiens 80-83 14634084-9 2003 Finally, CD25(+) T cells isolated from peripheral blood of nickel-allergic patients showed a limited or absent capacity to suppress metal-specific CD4(+) and CD8(+) T cell responses. Nickel 59-65 CD4 molecule Homo sapiens 147-150 15270870-10 2004 CONCLUSIONS: These findings demonstrate that freshly isolated nickel-reactive T cells can be characterized as CD4+ CLA+ memory T cells which express the chemokine receptors CXCR3, CCR4 and CCR10, but not CCR6. Nickel 62-68 CD4 molecule Homo sapiens 110-113 12060402-0 2002 Nickel-specific CD4(+) and CD8(+) T cells display distinct migratory responses to chemokines produced during allergic contact dermatitis. Nickel 0-6 CD4 molecule Homo sapiens 16-19 10644023-5 2000 After iontophoresis with nickel sulfate, only individuals sensitized to nickel reacted with a positive clinical response, increase in cutaneous blood flow, decline in epidermal CD-1a-positive cells, increase in epidermal proliferation (Ki-67-positive cells), pronounced infiltration of cells positive for CD4, CD11, or CLA, and cellular activation (expression of ICAM1, HLA-DR). Nickel 25-31 CD4 molecule Homo sapiens 305-308 11145678-1 2001 We have investigated the chemokine receptor expression and migratory behavior of a new subset of nickel-specific skin-homing regulatory CD4(+) T cells (Th(IL-10)) releasing high levels of IL-10, low IFN-gamma, and undetectable IL-4. Nickel 97-103 CD4 molecule Homo sapiens 136-139 10903743-5 2000 Nickel-specific CD4+ and CD8+ T cell lines established from ACD skin produced IFN-gamma and IL-4 and expressed moderate to high levels of CXCR3. Nickel 0-6 CD4 molecule Homo sapiens 16-19 10903743-6 2000 Finally, CXCR3 agonistic chemokines released by stimulated keratinocytes triggered calcium mobilization in skin-derived nickel-specific CD4+ T cells and promoted their migration, with supernatant from keratinocyte cultures stimulated with IFN-gamma and IL-4 attracting more efficaciously than supernatant from keratinocytes activated with IFN-gamma alone. Nickel 120-126 CD4 molecule Homo sapiens 136-139 10651989-0 2000 Human CD4+ T lymphocytes with remarkable regulatory functions on dendritic cells and nickel-specific Th1 immune responses. Nickel 85-91 CD4 molecule Homo sapiens 6-9 10651989-3 2000 Here, we examined the properties of a subset of nickel-specific CD4+ T cells displaying the cytokine profile (IL-10 , IL-5 , IFN-gamma+/-, IL-4+/-) of T regulatory cells 1 (Tr1) and with the potential to down-modulate immune responses to nickel. Nickel 48-54 CD4 molecule Homo sapiens 64-67 10651989-3 2000 Here, we examined the properties of a subset of nickel-specific CD4+ T cells displaying the cytokine profile (IL-10 , IL-5 , IFN-gamma+/-, IL-4+/-) of T regulatory cells 1 (Tr1) and with the potential to down-modulate immune responses to nickel. Nickel 238-244 CD4 molecule Homo sapiens 64-67