PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 21821009-4 2011 With prolonged exposure to nocodazole, a microtubule-depolymerizing agent, p62-deficient HCT116 cells exhibited an accumulation of a polyploid population with a limited appearance of apoptotic cells, which was attributable to the attenuated stabilization of p53. Nocodazole 27-37 tumor protein p53 Homo sapiens 258-261 8521385-0 1995 Microtubule-active drugs taxol, vinblastine, and nocodazole increase the levels of transcriptionally active p53. Nocodazole 49-59 tumor protein p53 Homo sapiens 108-111 8521385-2 1995 We examined the ability of three microtubule-active agents, taxol, vinblastine, and nocodazole, to increase p53 levels and activate p53-dependent processes. Nocodazole 84-94 tumor protein p53 Homo sapiens 108-111 8521385-2 1995 We examined the ability of three microtubule-active agents, taxol, vinblastine, and nocodazole, to increase p53 levels and activate p53-dependent processes. Nocodazole 84-94 tumor protein p53 Homo sapiens 132-135 25762091-5 2015 RESULTS: Here, we show that expression of Rap2B was induced by nocodazole in a p53-dependent manner. Nocodazole 63-73 tumor protein p53 Homo sapiens 79-82 8416202-3 1993 When the cells were arrested in mitotic metaphase by the use of nocodazole, p34cdc2 kinase activity was induced and p53 was apparently phosphorylated. Nocodazole 64-74 tumor protein p53 Homo sapiens 116-119 30482390-2 2019 The present study identified a novel pathway of p53 accumulation by SIRT2 suppression in HCT116(p53+/+) cells in which SIRT2 suppression led to escape from mitotic cell death caused by spindle assembly checkpoint activation induced by microtubule inhibitors such as nocodazole but not apoptosis or G1 or G2 arrest. Nocodazole 266-276 tumor protein p53 Homo sapiens 48-51 30482390-2 2019 The present study identified a novel pathway of p53 accumulation by SIRT2 suppression in HCT116(p53+/+) cells in which SIRT2 suppression led to escape from mitotic cell death caused by spindle assembly checkpoint activation induced by microtubule inhibitors such as nocodazole but not apoptosis or G1 or G2 arrest. Nocodazole 266-276 tumor protein p53 Homo sapiens 96-99 30482390-6 2019 Thus, the P/CAF-MDM2-p53-p21 axis enables the escape from mitotic cell death and confers resistance to nocodazole in HCT116(p53+/+) cells with SIRT2 suppression. Nocodazole 103-113 tumor protein p53 Homo sapiens 21-24 30482390-6 2019 Thus, the P/CAF-MDM2-p53-p21 axis enables the escape from mitotic cell death and confers resistance to nocodazole in HCT116(p53+/+) cells with SIRT2 suppression. Nocodazole 103-113 tumor protein p53 Homo sapiens 124-127 30126368-6 2018 Flow cytometry results revealed inhibition of ultraviolet radiation (UV)- and ionizing radiation (IR)- induced apoptosis and disruption of TP53-mediated cell cycle arrest after UV, IR and Nocodazole treatment. Nocodazole 188-198 tumor protein p53 Homo sapiens 139-143 18400748-4 2008 We found that after prolonged nocodazole exposure, the postmitotic checkpoint was facilitated by p53. Nocodazole 30-40 tumor protein p53 Homo sapiens 97-100 21559451-8 2011 Nocodazole-mediated cell-cycle arrest was accompanied by higher rate of apoptosis and upregulation of p53. Nocodazole 0-10 tumor protein p53 Homo sapiens 102-105 19934326-2 2009 Nocodazole-mediated aneuploidy was increased in p53-defective (p53Mut) cells; however, it was not increased in p53 wild-type (p53WT) cells. Nocodazole 0-10 tumor protein p53 Homo sapiens 48-51 19934326-2 2009 Nocodazole-mediated aneuploidy was increased in p53-defective (p53Mut) cells; however, it was not increased in p53 wild-type (p53WT) cells. Nocodazole 0-10 tumor protein p53 Homo sapiens 63-66 19332559-3 2009 p53 has long been known to be activated by spindle poisons, such as nocodazole and Taxol, although the underlying mechanism remains elusive. Nocodazole 68-78 tumor protein p53 Homo sapiens 0-3 21708134-3 2011 Treatment with the cell cycle inhibitors, aphidicolin or nocodazole also revealed increased NDRG1 phosphorylation in p53-deficient cells. Nocodazole 57-67 tumor protein p53 Homo sapiens 117-120 22022534-5 2011 METHODOLOGY: During induction of senescence by low levels of endogenous p53 and ectopic p21, cells were co-treated with nocodazole, which eliminated proliferating cells. Nocodazole 120-130 tumor protein p53 Homo sapiens 72-75 16945015-3 2006 Thus, nocodazole-treated cells were fluorescently labeled for different cell cycle-associated properties, including DNA content, retinoblastoma (Rb) and histone H3 phosphorylation, p53 and p21(WAF1) expression, nuclear and cell sizes, and cell morphology, and automatically imaged, analyzed, and correlated using HCA. Nocodazole 6-16 tumor protein p53 Homo sapiens 181-184 17015431-5 2006 Nocodazole, a microtubule poison that provokes centrosome/mitotic apparatus dysfunction, induces Lats2 translocation from centrosomes to the nucleus and p53 accumulation. Nocodazole 0-10 tumor protein p53 Homo sapiens 153-156 17015431-7 2006 Abrogation of Lats2 promotes accumulation of polyploid cells upon exposure to nocodazole, which can be prevented by direct activation of p53. Nocodazole 78-88 tumor protein p53 Homo sapiens 137-140 14583461-11 2003 Abnormal S10P in p53-/- cells was also observed under completely different experimental conditions where cells were treated with nocodazole to induce G(2)-M arrest and elevation of S10P (which is linked with G(2)-M of the cell cycle). Nocodazole 129-139 tumor protein p53 Homo sapiens 17-20 16319535-5 2006 When mitotic spindle is disrupted by nocodazole, ATM is displaced from centrosomes and colocalizes with phospho-Ser15-p53 under the form of spots dispersed in the mitotic cytoplasm. Nocodazole 37-47 tumor protein p53 Homo sapiens 118-121 16319535-6 2006 After release from nocodazole-block, as soon as cells exit mitosis, p53 is redirected to the nucleus and its Ser15 phosphorylation is substituted by phosphorylation at Ser46. Nocodazole 19-29 tumor protein p53 Homo sapiens 68-71 16687915-4 2006 If a cell stays in mitosis too long, (e.g. mitotic arrest caused by Taxol or nocodazole), then p53 accumulates. Nocodazole 77-87 tumor protein p53 Homo sapiens 95-98 14583461-12 2003 On removal of nocodazole, the p53+/+ cells exhibited rapid reduction in S10P levels and cell cycle recovery. Nocodazole 14-24 tumor protein p53 Homo sapiens 30-33 11313973-4 2001 By prolonged nocodazole treatment, U251MG human glioma cell, which has a p53 mutation, underwent transient arrest at mitosis, and subsequently exited from mitotic arrest (termed "mitotic slippage") followed by DNA replication without cytokinesis, resulting in hyperploid formation. Nocodazole 13-23 tumor protein p53 Homo sapiens 73-76 12860987-1 2003 Modification-specific antibodies were used to characterize the phosphorylation and acetylation of human p53 in response to genotoxic (UV, IR, and adriamycin) and non-genotoxic (PALA, taxol, nocodazole) stress in cultured human cells at 14 known modification sites. Nocodazole 190-200 tumor protein p53 Homo sapiens 104-107 12860987-5 2003 The non-genotoxic agents PALA, taxol and nocodazole induced p53 accumulation and phosphorylation at Ser6, Ser33, Ser46, and Ser392. Nocodazole 41-51 tumor protein p53 Homo sapiens 60-63 12221076-0 2002 Nocodazole-induced p53-dependent c-Jun N-terminal kinase activation reduces apoptosis in human colon carcinoma HCT116 cells. Nocodazole 0-10 tumor protein p53 Homo sapiens 19-22 12221076-4 2002 With the loss of the p53 gene, the levels of phosphorylation of Ser-63 of c-Jun and Thr-183/Tyr-185 of JNK1/2 in p53-/- cells did not increase as markedly as in p53+/+ cells in response to a 1-h treatment with nocodazole or other microtubule-disrupting drugs such as vinblastine and colchicine. Nocodazole 210-220 tumor protein p53 Homo sapiens 21-24 12221076-4 2002 With the loss of the p53 gene, the levels of phosphorylation of Ser-63 of c-Jun and Thr-183/Tyr-185 of JNK1/2 in p53-/- cells did not increase as markedly as in p53+/+ cells in response to a 1-h treatment with nocodazole or other microtubule-disrupting drugs such as vinblastine and colchicine. Nocodazole 210-220 tumor protein p53 Homo sapiens 113-116 12221076-4 2002 With the loss of the p53 gene, the levels of phosphorylation of Ser-63 of c-Jun and Thr-183/Tyr-185 of JNK1/2 in p53-/- cells did not increase as markedly as in p53+/+ cells in response to a 1-h treatment with nocodazole or other microtubule-disrupting drugs such as vinblastine and colchicine. Nocodazole 210-220 tumor protein p53 Homo sapiens 113-116 12221076-7 2002 Inhibition of p53 expression by its antisense oligonucleotide also attenuated nocodazole-induced JNK activation in p53+/+ cells. Nocodazole 78-88 tumor protein p53 Homo sapiens 14-17 12221076-7 2002 Inhibition of p53 expression by its antisense oligonucleotide also attenuated nocodazole-induced JNK activation in p53+/+ cells. Nocodazole 78-88 tumor protein p53 Homo sapiens 115-118 12221076-8 2002 Surprisingly, cotransfection of p53+/+ cells with dominant negative mutants of JNK isoforms and treatment of p53+/+ cells with the JNK inhibitor SP600125 actually further enhanced apoptosis in p53+/+ cells by up to 2-fold in response to nocodazole. Nocodazole 237-247 tumor protein p53 Homo sapiens 32-35 12221076-8 2002 Surprisingly, cotransfection of p53+/+ cells with dominant negative mutants of JNK isoforms and treatment of p53+/+ cells with the JNK inhibitor SP600125 actually further enhanced apoptosis in p53+/+ cells by up to 2-fold in response to nocodazole. Nocodazole 237-247 tumor protein p53 Homo sapiens 109-112 12221076-8 2002 Surprisingly, cotransfection of p53+/+ cells with dominant negative mutants of JNK isoforms and treatment of p53+/+ cells with the JNK inhibitor SP600125 actually further enhanced apoptosis in p53+/+ cells by up to 2-fold in response to nocodazole. Nocodazole 237-247 tumor protein p53 Homo sapiens 109-112 11841447-5 2002 p53-transfected and control cells were treated with etoposide and trapped at mitosis with nocodazole. Nocodazole 90-100 tumor protein p53 Homo sapiens 0-3 11376010-2 2001 Here we report that a transient inhibition of spindle assembly induced by nocodazole, a tubulin-depolymerizing drug, triggers a stable activation of p53, which can transduce a cell cycle inhibitory signal even when the spindle-damaging agent is removed and the spindle is allowed to reassemble. Nocodazole 74-84 tumor protein p53 Homo sapiens 149-152 11376010-3 2001 Cells transiently exposed to nocodazole continue to express high levels of p53 and p21 in the cell cycle that follows the transient exposure to nocodazole and become arrested in G(1), regardless of whether they carry a diploid or polyploid genome after mitotic exit. Nocodazole 29-39 tumor protein p53 Homo sapiens 75-78 11376010-3 2001 Cells transiently exposed to nocodazole continue to express high levels of p53 and p21 in the cell cycle that follows the transient exposure to nocodazole and become arrested in G(1), regardless of whether they carry a diploid or polyploid genome after mitotic exit. Nocodazole 144-154 tumor protein p53 Homo sapiens 75-78 11313973-6 2001 By employing LN382 glioma cell that has a temperature-sensitive p53 mutation, we found that the activation of p53 prevents hyperploid formation after the prolonged nocodazole treatment. Nocodazole 164-174 tumor protein p53 Homo sapiens 64-67 11313973-6 2001 By employing LN382 glioma cell that has a temperature-sensitive p53 mutation, we found that the activation of p53 prevents hyperploid formation after the prolonged nocodazole treatment. Nocodazole 164-174 tumor protein p53 Homo sapiens 110-113 9840938-4 1998 To learn if transcriptional activation of downstream genes by p53 plays a role in this putative checkpoint, three cell lines were exposed to nocodazole. Nocodazole 141-151 tumor protein p53 Homo sapiens 62-65 9840938-7 1998 Incubation with nocodazole of cells containing wild-type p53 results in accumulation of both 2N and 4N populations of cells. Nocodazole 16-26 tumor protein p53 Homo sapiens 57-60 11244509-6 2001 p53 phosphorylation also varied in a MTI-dependent manner, as Taxol and Vincristine induced more p53 phospho-forms than nocodazole. Nocodazole 120-130 tumor protein p53 Homo sapiens 0-3 11244509-9 2001 Analysis of ectopically expressed p53 phospho-mutant proteins from Taxol- and nocodazole-treated cells indicated that multiple p53 amino terminal residues, including serine-15 and threonine-18, were required for Taxol-mediated phosphorylation of p53. Nocodazole 78-88 tumor protein p53 Homo sapiens 34-37 11244509-9 2001 Analysis of ectopically expressed p53 phospho-mutant proteins from Taxol- and nocodazole-treated cells indicated that multiple p53 amino terminal residues, including serine-15 and threonine-18, were required for Taxol-mediated phosphorylation of p53. Nocodazole 78-88 tumor protein p53 Homo sapiens 127-130 11244509-9 2001 Analysis of ectopically expressed p53 phospho-mutant proteins from Taxol- and nocodazole-treated cells indicated that multiple p53 amino terminal residues, including serine-15 and threonine-18, were required for Taxol-mediated phosphorylation of p53. Nocodazole 78-88 tumor protein p53 Homo sapiens 127-130