PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 21699887-7 2011 RESULTS: An activation step consisting in prolidase incubation with 1 mmol/l MnCl(2) and 0.75 mmol/l reduced glutathione at 50 C for 20 min was necessary to obtain the maximum activity and to accurately determine, for the recombinant enzyme, V(max) (489 U/mg), K(m) (5.4 mM) and Mn(2+) affinity (54 mM(-1)). Glutathione 109-120 peptidase D Homo sapiens 42-51