PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 19826089-4 2009 We now report reconstitution of lysis-free membrane fusion that requires purified GTP-bound Ypt7p, HOPS complex, vacuolar SNAREs, ATP hydrolysis, and the SNARE disassembly catalysts Sec17p and Sec18p. Guanosine Triphosphate 82-85 AAA family ATPase SEC18 Saccharomyces cerevisiae S288C 193-199 9382859-2 1997 Purified Sec18p, Uso1p, and LMA1 in the presence of ATP and GTP satisfies the requirement for cytosol in fusion of ER-derived vesicles with Golgi membranes. Guanosine Triphosphate 60-63 AAA family ATPase SEC18 Saccharomyces cerevisiae S288C 9-15