PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 15549291-6 2005 The resulting two-fold recombinant strain exhibited an in vitro catalytic alcohol dehydrogenase activity of 6.5 units mg-1 protein in reducing methyl acetoacetate to methyl (R)-3-hydroxy butanoate with NADPH as the cofactor and 0.7 units mg-1 protein with NADH. NAD 256-260 Alcohol dehydrogenase Escherichia coli 74-95 23435875-3 2013 In this study, E. coli SZ420 was further engineered for reduction of xylose to xylitol by (1) deleting the alcohol dehydrogenase gene (adhE) to divert NADH from the ethanol pathway; (2) deleting the glucose-specific PTS permease gene (ptsG) to eliminate catabolite repression and allow simultaneous uptake of glucose and xylose; (3) cloning the aldose reductase gene (xylI) of Candida boidinii to reduce xylose to xylitol. NAD 151-155 Alcohol dehydrogenase Escherichia coli 107-128 15549291-11 2005 Thus, the functional overexpression of alcohol dehydrogenase in the presence of formate dehydrogenase was sufficient to enable and sustain the desired reduction reaction via the relatively low specific activity of alcohol dehydrogenase with NADH, instead of NADPH, as a cofactor. NAD 241-245 Alcohol dehydrogenase Escherichia coli 39-60 15549291-11 2005 Thus, the functional overexpression of alcohol dehydrogenase in the presence of formate dehydrogenase was sufficient to enable and sustain the desired reduction reaction via the relatively low specific activity of alcohol dehydrogenase with NADH, instead of NADPH, as a cofactor. NAD 241-245 Alcohol dehydrogenase Escherichia coli 214-235 8020473-0 1994 A few amino acid substitutions are responsible for the higher thermostability of a novel NAD(+)-dependent bacillar alcohol dehydrogenase. NAD 89-95 Alcohol dehydrogenase Escherichia coli 115-136 8020473-1 1994 The gene adh-hT encoding a thermostable and thermophilic NAD(+)-dependent alcohol dehydrogenase (ADH) from the novel and more thermophilic Bacillus stearothermophilus LLD-R strain was cloned and its nucleotide sequence determined. NAD 57-63 Alcohol dehydrogenase Escherichia coli 9-12 8020473-1 1994 The gene adh-hT encoding a thermostable and thermophilic NAD(+)-dependent alcohol dehydrogenase (ADH) from the novel and more thermophilic Bacillus stearothermophilus LLD-R strain was cloned and its nucleotide sequence determined. NAD 57-63 Alcohol dehydrogenase Escherichia coli 74-95 8020473-1 1994 The gene adh-hT encoding a thermostable and thermophilic NAD(+)-dependent alcohol dehydrogenase (ADH) from the novel and more thermophilic Bacillus stearothermophilus LLD-R strain was cloned and its nucleotide sequence determined. NAD 57-63 Alcohol dehydrogenase Escherichia coli 97-100