PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 8157576-4 1994 It is generally assumed that the mecA gene product (PBP 2A) acts as a surrogate enzyme which takes over the task of cell wall synthesis from the normal complement of staphylococcal PBPs, since the latter are inhibited by relatively low (e.g. methicillin) concentrations of beta-lactam antibiotics. Methicillin 242-253 AT695_RS11765 Staphylococcus aureus 52-55 30008711-5 2018 Detection of meticillin resistance is relatively straightforward since it is defined by a single determinant, penicillin-binding protein 2a", which exists in a limited number of genetic variants carried on various Staphylococcal Cassette Chromosomes mec. Methicillin 13-23 AT695_RS11765 Staphylococcus aureus 110-136 34301077-2 2021 MRSA strains are resistant to methicillin due to the formation of beta-lactamase enzymes, as well as the acquisition of the mecA gene, which encodes the penicillin-binding protein (PBP2a) that reduces the affinity for beta-lactam drugs. Methicillin 30-41 AT695_RS11765 Staphylococcus aureus 153-179 2610497-0 1989 New mechanism for methicillin resistance in Staphylococcus aureus: clinical isolates that lack the PBP 2a gene and contain normal penicillin-binding proteins with modified penicillin-binding capacity. Methicillin 18-29 AT695_RS11765 Staphylococcus aureus 99-102 2610497-6 1989 Strain CDC-1, prototype of heterogeneous methicillin-resistant S. aureus, contained penicillin-binding protein (PBP) 2a; its DNA could transform a methicillin-susceptible and novobiocin-resistant recipient to methicillin resistance with ca. Methicillin 41-52 AT695_RS11765 Staphylococcus aureus 84-110 2610497-6 1989 Strain CDC-1, prototype of heterogeneous methicillin-resistant S. aureus, contained penicillin-binding protein (PBP) 2a; its DNA could transform a methicillin-susceptible and novobiocin-resistant recipient to methicillin resistance with ca. Methicillin 41-52 AT695_RS11765 Staphylococcus aureus 112-115 2610497-6 1989 Strain CDC-1, prototype of heterogeneous methicillin-resistant S. aureus, contained penicillin-binding protein (PBP) 2a; its DNA could transform a methicillin-susceptible and novobiocin-resistant recipient to methicillin resistance with ca. Methicillin 147-158 AT695_RS11765 Staphylococcus aureus 84-110 2610497-6 1989 Strain CDC-1, prototype of heterogeneous methicillin-resistant S. aureus, contained penicillin-binding protein (PBP) 2a; its DNA could transform a methicillin-susceptible and novobiocin-resistant recipient to methicillin resistance with ca. Methicillin 147-158 AT695_RS11765 Staphylococcus aureus 112-115 2536684-4 1989 Next, we isolated a methicillin-susceptible mutant from a highly and homogeneously resistant strain which had a Tn551 insertion near or within the PBP 2a gene and thus did not produce PBP 2a. Methicillin 20-31 AT695_RS11765 Staphylococcus aureus 147-150 3435121-3 1987 In the heterogeneous methicillin-resistant strain DU4916-K7, less PBP 2" was produced at 40 degrees C than at 30 degrees C, but inclusion of 5% (wt/vol) NaCl in the medium at 40 degrees C boosted PBP 2" production and allowed growth of the organism in the presence of 10 micrograms of methicillin per ml. Methicillin 21-32 AT695_RS11765 Staphylococcus aureus 66-69 3435121-3 1987 In the heterogeneous methicillin-resistant strain DU4916-K7, less PBP 2" was produced at 40 degrees C than at 30 degrees C, but inclusion of 5% (wt/vol) NaCl in the medium at 40 degrees C boosted PBP 2" production and allowed growth of the organism in the presence of 10 micrograms of methicillin per ml. Methicillin 21-32 AT695_RS11765 Staphylococcus aureus 196-199 3435121-4 1987 When exponential-phase cultures were challenged with methicillin, growth and peptidoglycan synthesis were much more resistant at 30 degrees C than at 40 degrees C. Inclusion of NaCl in medium rendered growth and peptidoglycan synthesis more methicillin resistant at 40 degrees C. Hence, there was a good correlation between PBP 2" production and methicillin-resistant peptidoglycan synthesis under these conditions. Methicillin 53-64 AT695_RS11765 Staphylococcus aureus 324-327 3435121-6 1987 Pregrowth of cells with methicillin, which was expected to boost PBP 2" production, seemed to increase the susceptibilities of growth and peptidoglycan synthesis to methicillin. Methicillin 24-35 AT695_RS11765 Staphylococcus aureus 65-68 3435121-6 1987 Pregrowth of cells with methicillin, which was expected to boost PBP 2" production, seemed to increase the susceptibilities of growth and peptidoglycan synthesis to methicillin. Methicillin 165-176 AT695_RS11765 Staphylococcus aureus 65-68 3435121-10 1987 It appears that NaCl enhances methicillin resistance by stimulating PBP 2" production and providing osmotic support but opposes it by stimulating autolytic activity which is exacerbated by the very low cross-linking of peptidoglycan in methicillin-resistant strains grown in the presence of methicillin. Methicillin 30-41 AT695_RS11765 Staphylococcus aureus 68-71 25182856-2 2014 Resistance to methicillin is related to production of penicillin binding protein 2a (PBP2a). Methicillin 14-25 AT695_RS11765 Staphylococcus aureus 54-80 21106176-3 2010 Oxacillin remains active against these strains, but hospital associated staphylococci and more recently community acquired staphylococci have developed crossed resistance between methicillin (MRSA), oxacillin and other beta-lactams by production of a penicillin binding protein (PBP) with low affinity for beta-lactams, PBP2a. Methicillin 179-190 AT695_RS11765 Staphylococcus aureus 251-277 21106176-3 2010 Oxacillin remains active against these strains, but hospital associated staphylococci and more recently community acquired staphylococci have developed crossed resistance between methicillin (MRSA), oxacillin and other beta-lactams by production of a penicillin binding protein (PBP) with low affinity for beta-lactams, PBP2a. Methicillin 179-190 AT695_RS11765 Staphylococcus aureus 279-282 22587932-1 2012 Discrimination of methicillin resistant (MRSA) and sensitive (MSSA) strains of Staphylococcus aureus, was achieved by the specially selected lytic bacteriophage with a wide host range of S. aureus strains and a penicillin-binding protein (PBP 2a) specific antibody. Methicillin 18-29 AT695_RS11765 Staphylococcus aureus 211-237 20129965-0 2010 Direct detection of methicillin resistance in Staphylococcus aureus in blood culture broth by use of a penicillin binding protein 2a latex agglutination test. Methicillin 20-31 AT695_RS11765 Staphylococcus aureus 103-129 20129965-1 2010 We studied the utility of performing a penicillin binding protein 2a latex agglutination (PBP-LA) assay directly on Bactec blood culture broth samples containing Staphylococcus aureus to rapidly detect methicillin resistance. Methicillin 202-213 AT695_RS11765 Staphylococcus aureus 39-65