PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 30942074-4 2019 In the real-time fluorescence imaging of apoptotic HeLa cells induced by staurosporine using GNP-Se-Casp, the fluorescence signals corresponding to casp-8 and casp-9 sequentially turn on, followed by the appearance of the fluorescence of casp-3, which visualizes the upstream and downstream relationships of casp-3, -8, and -9. Staurosporine 73-86 caspase 3 Homo sapiens 238-244 30942074-4 2019 In the real-time fluorescence imaging of apoptotic HeLa cells induced by staurosporine using GNP-Se-Casp, the fluorescence signals corresponding to casp-8 and casp-9 sequentially turn on, followed by the appearance of the fluorescence of casp-3, which visualizes the upstream and downstream relationships of casp-3, -8, and -9. Staurosporine 73-86 caspase 3 Homo sapiens 308-326 30488656-11 2019 Treatment of patient cells with MG-132 or staurosporine to induce activation of the intrinsic apoptosis pathway revealed significantly decreased cell viability with increased caspase-3 and caspase-7 activation. Staurosporine 42-55 caspase 3 Homo sapiens 175-184 29107935-8 2018 These compounds also activated caspase-3 but an apoptosis-like type of cell death was unlike since PARP protein was not cleaved and caspase activation was substantially lower than its activation induced by staurosporine, a known caspase-3 activator in T24 cells. Staurosporine 206-219 caspase 3 Homo sapiens 229-238 30289248-11 2019 Meanwhile, caspase-3 in staurosporine-treated A549 cells are also determined by the approach, and the obtained results agree well with the fluorescent intensity of confocal images, manifesting that the proposed PEC method can monitor apoptosis in a label-free strategy. Staurosporine 24-37 caspase 3 Homo sapiens 11-20 29039468-6 2017 However, the Abeta42-induced activation of the IAPW differed from that induced by treatment with other agents, such as staurosporine (STS) in that lower amounts of cytochrome c were released from the mitochondria, the majority of procaspase-9 in the Apaf-1 complex was not processed and caspase-3 was activated to a lesser extent in the peptide-treated cells. Staurosporine 119-132 caspase 3 Homo sapiens 287-296 29039468-6 2017 However, the Abeta42-induced activation of the IAPW differed from that induced by treatment with other agents, such as staurosporine (STS) in that lower amounts of cytochrome c were released from the mitochondria, the majority of procaspase-9 in the Apaf-1 complex was not processed and caspase-3 was activated to a lesser extent in the peptide-treated cells. Staurosporine 134-137 caspase 3 Homo sapiens 287-296 28942148-7 2017 High glucose-derived EMPs significantly increased both basal (1.5 +- 0.1 vs 1.0 +- 0.1 ng/mL) and staurosporine-stimulated (2.2 +- 0.2 vs 1.4 +- 0.1 ng/mL) active caspase-3 compared with normal glucose EMPs. Staurosporine 98-111 caspase 3 Homo sapiens 163-172 25761242-3 2015 Upon application of staurosporine and to some extent after treatment with phorbol-12-myristate-13-acetate (PMA), a specific activator of protein kinase c, the caspase-3 sensitive peptide linker DEVD is cleaved. Staurosporine 20-33 caspase 3 Homo sapiens 159-168 28787459-3 2017 Using cultured hippocampal slices and primary neurons we show that a typical apoptosis inducer-staurosporine (STP) was able to cause concentration-dependent apoptotic cell death in brain slices; Enhanced synaptic activity by bicuculline (Bic)/4-Aminopyridine (AP) treatment effectively prevented neurons from STP-induced cell apoptosis, as indicated by increased cell survival and suppressed caspase-3 activity. Staurosporine 95-108 caspase 3 Homo sapiens 392-401 26963176-5 2016 WGMBs are functionalized with antibodies for the specific and label-free detection of procaspase-3 released from human embryonic kidney HEK293 and neuroglioma H4 cells after introducing staurosporine and rotenone toxins, respectively. Staurosporine 186-199 caspase 3 Homo sapiens 86-98 26493876-5 2015 HeLa cells electroporated with S1 -P1 are able to deliver the cargo in the presence of staurosporin (STS), which induces apoptosis with the consequent activation of the cytoplasmic C3 enzyme. Staurosporine 87-99 caspase 3 Homo sapiens 181-183 26493876-5 2015 HeLa cells electroporated with S1 -P1 are able to deliver the cargo in the presence of staurosporin (STS), which induces apoptosis with the consequent activation of the cytoplasmic C3 enzyme. Staurosporine 101-104 caspase 3 Homo sapiens 181-183 25998538-8 2015 Furthermore, alphaB-crystallin/HSPB5 decreases the staurosporine-mediated cleavage of caspase 3 through PI3K/Akt signaling preventing apoptosis of astrocytes. Staurosporine 51-64 caspase 3 Homo sapiens 86-95 25464147-5 2015 After predetermining the gamma(458) of Venus to ECFP, we used sp-ECR method to monitor the staurosporine (STS)-induced dynamical caspase-3 activation in single live A549 cells expressing SCAT3 by spectrally resolving the absolute FRET efficiency of SCAT3, and found that STS-induced caspase-3 activation in single cells is a very rapid process within 20 min. Staurosporine 91-104 caspase 3 Homo sapiens 129-138 25464147-5 2015 After predetermining the gamma(458) of Venus to ECFP, we used sp-ECR method to monitor the staurosporine (STS)-induced dynamical caspase-3 activation in single live A549 cells expressing SCAT3 by spectrally resolving the absolute FRET efficiency of SCAT3, and found that STS-induced caspase-3 activation in single cells is a very rapid process within 20 min. Staurosporine 91-104 caspase 3 Homo sapiens 283-292 25464147-5 2015 After predetermining the gamma(458) of Venus to ECFP, we used sp-ECR method to monitor the staurosporine (STS)-induced dynamical caspase-3 activation in single live A549 cells expressing SCAT3 by spectrally resolving the absolute FRET efficiency of SCAT3, and found that STS-induced caspase-3 activation in single cells is a very rapid process within 20 min. Staurosporine 106-109 caspase 3 Homo sapiens 129-138 24422709-6 2014 Staurosporine-stimulated activation of caspase-3 was also similar between the low (2.3 +- 0.2 ng/mL), moderate (2.1 +- 0.3 ng/mL), and high (2.2 +- 0.2 ng/mL) CRP groups. Staurosporine 0-13 caspase 3 Homo sapiens 39-48 24885713-9 2014 RESULTS: Caspase-3 expression in staurosporine-incubated cells increased by 471% +- 182% compared to control (P=0.014). Staurosporine 33-46 caspase 3 Homo sapiens 9-18 24378649-4 2014 Here, we found firstly that staurosporine-induced apoptosis, detected as cleavage of caspase 3 is more than 3-times higher in beta-arrestin 1-lacking astrocytes than in control cells. Staurosporine 28-41 caspase 3 Homo sapiens 85-94 24421315-7 2014 PDGF-C prevented staurosporine-induced macrophage apoptosis by inhibiting the activation of caspase-3, -7, -8, and -9 and cleavage of poly(ADP-ribose) polymerase. Staurosporine 17-30 caspase 3 Homo sapiens 92-117 24219236-7 2014 In addition, both compounds were able to reduce caspase-3 activity induced by the apoptotic enhancer staurosporine, but undecylprodigiosin failed to inhibit FCCP effects and it did not act over the Nrf2 pathway as was the case for anhydroexfoliamycin. Staurosporine 101-114 caspase 3 Homo sapiens 48-57 24091666-11 2013 Flow cytometric analysis proved that pJAB1 significantly enhanced apoptosis induced by staurosporine, which at least partially depended on the activation of caspase-9 and caspase-3. Staurosporine 87-100 caspase 3 Homo sapiens 171-180 24128664-5 2014 When exposed to staurosporine, fibroblasts from patients also showed higher caspase-3 activity; a higher percentage of cells with translocated phosphatidylserine and condensed chromatin; and higher p53 expression compared to fibroblasts from controls. Staurosporine 16-29 caspase 3 Homo sapiens 76-85 23643731-6 2013 Following induction of mitochondrial biogenesis, L6 myoblasts displayed decreased sensitivity to apoptotic cell death as well as reduced caspase-3 and caspase-9 activation following exposure to staurosporine (STS) and C2-ceramide. Staurosporine 194-207 caspase 3 Homo sapiens 137-146 23643731-6 2013 Following induction of mitochondrial biogenesis, L6 myoblasts displayed decreased sensitivity to apoptotic cell death as well as reduced caspase-3 and caspase-9 activation following exposure to staurosporine (STS) and C2-ceramide. Staurosporine 209-212 caspase 3 Homo sapiens 137-146 23097134-4 2013 Using human neuroblastoma cell lines, SK-N-MC, SH-SY5Y, and SK-N-SH, we show that non-toxic dose (2 mM) of VPA enhanced staurosporine (STS)-induced cell death as assessed by MTT assay, PARP cleavage, hypodiploidy, and caspase 3 activity. Staurosporine 120-133 caspase 3 Homo sapiens 218-227 23667252-10 2013 It also increases staurosporine-induced caspase-3/7 activity, which is rescued by dendrin depletion in YAP knockdown cells. Staurosporine 18-31 caspase 3 Homo sapiens 40-49 23333591-3 2013 STS treatment, without T. gondii infection, reduced the viability of THP-1 cells in proportion to STS concentration and triggered many cellular death events such as caspase-3 and -9 activation, Bax translocation, cytochrome c release from host cell mitochondria into cytosol, and PARP cleavage in the host cell. Staurosporine 0-3 caspase 3 Homo sapiens 165-181 23097134-4 2013 Using human neuroblastoma cell lines, SK-N-MC, SH-SY5Y, and SK-N-SH, we show that non-toxic dose (2 mM) of VPA enhanced staurosporine (STS)-induced cell death as assessed by MTT assay, PARP cleavage, hypodiploidy, and caspase 3 activity. Staurosporine 135-138 caspase 3 Homo sapiens 218-227 23122728-4 2013 We detected a robust increase in calcium levels in response to staurosporine treatment in primary human fibroblasts and HeLa cells in the presence of the caspase inhibitor Z-VAD, indicating that calcium release during the initiation of apoptosis occurs independently of caspase 3. Staurosporine 63-76 caspase 3 Homo sapiens 270-279 22460504-3 2012 Caspase-3 activation and DNA laddering induced by staurosporine were abolished by blockers of K(+) and Cl(-) channels or cytosolic Ca(2+) chelation. Staurosporine 50-63 caspase 3 Homo sapiens 0-9 22884615-6 2012 Also, 27-hydroxycholesterol significantly decreased the staurosporine-mediated induction of caspase-3 and -7, known to be important in apoptotic events. Staurosporine 56-69 caspase 3 Homo sapiens 92-108 22460504-4 2012 Staurosporine induced decreases in the intracellular free K(+) and Cl(-) concentrations ([K(+)](i) and [Cl(-)](i)) in an early stage prior to caspase-3 activation. Staurosporine 0-13 caspase 3 Homo sapiens 142-151 22285488-2 2012 Here we show that cleavage of Scythe by caspase-3 occurs after activation of both the extrinsic (i.e. Fas/APO-1-mediated) and the intrinsic (i.e. staurosporine-induced) apoptosis pathway. Staurosporine 146-159 caspase 3 Homo sapiens 40-49 22159547-6 2012 Dose-response profiles of caspase-3 activity as a function of staurosporine concentration were generated using both the digital microfluidic method and conventional techniques (i.e., pipetting, aspiration, and 96-well plates.) Staurosporine 62-75 caspase 3 Homo sapiens 26-35 22184125-4 2012 We first show that both recombinant sAPPalpha and N1, but not its inactive parent fragment N2, reduce staurosporine-stimulated caspase-3 activation and TUNEL-positive cell death by lowering p53 promoter transactivation and activity in human cells. Staurosporine 102-115 caspase 3 Homo sapiens 127-136 20869113-4 2010 Furthermore, we demonstrated that TRH and its analogues decreased the staurosporine (0.5 muM)-induced LDH release, caspase-3 activity and DNA fragmentation, which indicate the anti-apoptotic proprieties of these peptides. Staurosporine 70-83 caspase 3 Homo sapiens 115-124 21437645-3 2011 Although caspase-9 activation and cleavage of procaspase-3 were significant following staurosporine treatment, neither was observed following H(2)O(2) treatment, indicating a non-apoptotic death. Staurosporine 86-99 caspase 3 Homo sapiens 46-58 21219955-8 2011 By contrast, staurosporine affected both clean and infected cells, causing apoptotic damage (cell shrinkage, nuclear apoptotic alterations, caspase-3 activation and caspase-promoted proteolysis, without PI permeability, and without effect on XTT reduction). Staurosporine 13-26 caspase 3 Homo sapiens 140-149 20864561-8 2010 CD31(+) T cells from middle-aged and older men demonstrated greater apoptotic susceptibility, as staurosporine-stimulated intracellular caspase-3 activation was ~ 40% higher (P < 0.05) than young. Staurosporine 97-110 caspase 3 Homo sapiens 136-145 21237154-5 2011 These data suggest that TRAIL and staurosporine induce JNK activation in a caspase-3-independent manner and that caspase-3-mediated JIP1 cleavage plays a role in JNK inactivation via scaffold disassembly during the execution phase of apoptosis. Staurosporine 34-47 caspase 3 Homo sapiens 75-84 21625174-3 2011 Intracellular active caspase-3 concentrations in response to staurosporine stimulation were approximately 35% higher (p < 0.05) in EPCs from older (3.15 +- 0.29 pg/ml) compared with young (2.33 +- 0.24 pg/ml) men. Staurosporine 61-74 caspase 3 Homo sapiens 21-30 20057362-11 2010 Staurosporine induced a ~30% greater (P < 0.05) increase in active caspase-3 in EPCs from overweight/obese (2.8 +/- 0.2 ng/ml) compared with normal weight (2.2 +/- 0.2) subjects. Staurosporine 0-13 caspase 3 Homo sapiens 70-79 19840952-4 2010 METHODS AND RESULTS: CX3CL1 significantly reduces staurosporine-induced apoptosis of CASMC, as quantified by caspase 3 immunostaining and Annexin-V flow cytometry. Staurosporine 50-63 caspase 3 Homo sapiens 109-118 20385969-8 2010 Finally, TDGF-1 protected H295R cells from apoptosis induced by staurosporine, causing a decrease in caspase-3 activity, a reduction in the inactivation of poly(ADP-ribose) polymerase, and an inhibition of DNA fragmentation, detected by the TUNEL reaction and fluorescence microscopy that was blocked by LY294002. Staurosporine 64-77 caspase 3 Homo sapiens 101-110 19216720-5 2009 RESULTS: Staurosporine can induce death of HeLa cells via a cytochrome c/caspase-9/caspase-3 mitochondrial-dependent apoptotic pathway and via a delayed caspase-independent pathway. Staurosporine 9-22 caspase 3 Homo sapiens 83-92 20010439-8 2010 In particular, the triple combination of cytochalasin D+LY294002+olomoucine was almost as effective as staurosporine in inducing caspase-3 activity and apoptosis. Staurosporine 103-116 caspase 3 Homo sapiens 129-138 19582370-3 2009 Neither treatment induced caspase-7 activity, but caspase-3 was activated by staurosporine but not H2O2. Staurosporine 77-90 caspase 3 Homo sapiens 50-59 19135127-3 2009 Cadmium-induced activation of caspase-3 was significantly attenuated in HEK-sbGHS-R1a cells compared to wild-type HEK293 cells, while the apoptotic responses to the protein kinase C inhibitor staurosporine were similar. Staurosporine 192-205 caspase 3 Homo sapiens 30-39 19187443-7 2009 Interestingly, we also show that EC33 and pl302 lower staurosporine-stimulated activation of caspase-3 in wild-type fibroblasts but not in betaAPP/beta-amyloid precursor protein-like protein 2 (APLP2) double knockout fibroblasts, suggesting that protecting endogenous fl-Abeta physiological production triggers neuroprotective phenotype. Staurosporine 54-67 caspase 3 Homo sapiens 93-102 19261878-7 2009 Moreover, HDACIs also prevented caspase-3 cleavage in postnatal cortical neurons treated with staurosporine, 3-nitropropionic acid and a Bcl-2 inhibitor, all of which require the presence of Bax but not p53 to promote apoptosis. Staurosporine 94-107 caspase 3 Homo sapiens 32-41 19229559-6 2009 In lymphoblastoid cells carrying SCA8 large alleles, treatment of MG-132 or staurosporine significantly increases the cell death or caspase 3 activity. Staurosporine 76-89 caspase 3 Homo sapiens 132-141 18832097-4 2009 In the LNCaP prostate cancer cell line, induction of apoptosis and caspase-3/7 activities by staurosporine (STS) abolished [(3)H]1,25-dihydroxy vitamin D(3) binding and VDR protein, suggesting that the VDR may be targeted for inactivation by caspases during apoptosis. Staurosporine 93-106 caspase 3 Homo sapiens 67-76 18983913-4 2009 Treatment with the cytokine efficaciously protected mesoangioblasts from apoptosis induced by serum starvation or staurosporine treatment assessed by various means such as activation of caspase-3, determination of cytoplasmic histone-associated-DNA-fragments and PE-AnnexinV staining. Staurosporine 114-127 caspase 3 Homo sapiens 186-195 18832097-4 2009 In the LNCaP prostate cancer cell line, induction of apoptosis and caspase-3/7 activities by staurosporine (STS) abolished [(3)H]1,25-dihydroxy vitamin D(3) binding and VDR protein, suggesting that the VDR may be targeted for inactivation by caspases during apoptosis. Staurosporine 108-111 caspase 3 Homo sapiens 67-76 17340627-6 2007 Expression of active Akt targeted to mitochondria was found to be sufficient to significantly reduce staurosporine-induced activation of caspase-3 and caspase-9, the release of cytochrome c from mitochondria, and Bax oligomerization at mitochondria. Staurosporine 101-114 caspase 3 Homo sapiens 137-146 18594935-5 2008 However, upon treatment with staurosporine, BRAt cells showed increased levels of active caspase-3 and increased cleavage of caspase-3 substrates, PARP and DFF45. Staurosporine 29-42 caspase 3 Homo sapiens 89-98 18594935-5 2008 However, upon treatment with staurosporine, BRAt cells showed increased levels of active caspase-3 and increased cleavage of caspase-3 substrates, PARP and DFF45. Staurosporine 29-42 caspase 3 Homo sapiens 125-134 18953090-10 2008 Moreover, neurosteroids appear to attenuate the staurosporine-induced cell damage in a caspase-3 independent way and their neuroprotective mechanism of action involves the increase in ERK-MAPK phosphorylation. Staurosporine 48-61 caspase 3 Homo sapiens 87-96 18534571-3 2008 Staurosporine increased caspase 3-like activity, DNA fragmentation, PARP cleavage, and the number of TUNEL positive cells consistent with the induction of apoptosis. Staurosporine 0-13 caspase 3 Homo sapiens 24-33 18478334-4 2008 It was found that hypertonic stress reduces staurosporine-induced AVD and cell death (associated with caspase-3/7 activation and DNA fragmentation), and that this effect was actually due to activation of the HICC. Staurosporine 44-57 caspase 3 Homo sapiens 102-111 18405662-7 2008 However, we demonstrate that both staurosporine-stimulated caspase-3 activation, p53 and neprilysin expression and activity were not affected by TMP21 over-expression or depletion. Staurosporine 34-47 caspase 3 Homo sapiens 59-68 18497968-7 2008 AKAP149 was also cleaved by caspases during Fas- and staurosporine-induced apoptosis in Jurkat T and HeLa cells, which were blocked by specific inhibitors of caspase-3 and -8. Staurosporine 53-66 caspase 3 Homo sapiens 158-174 18840413-7 2009 STP-induced HT29 cell apoptosis was associated with caspase-3 activation independent of caspase-8 or caspase-9 activity; accordingly, inhibitors of the latter caspases were without effect on STP-induced apoptosis. Staurosporine 0-3 caspase 3 Homo sapiens 52-61 18840413-8 2009 STP similarly induced GSH efflux and apoptosis in a non-malignant human NCM460 colonic cell line in association with caspase-3 activation. Staurosporine 0-3 caspase 3 Homo sapiens 117-126 18787932-8 2009 Induction of apoptosis in U-937 cells by staurosporine or TNFalpha resulted in an increase in cyclin A1 protein expression, which correlated well with cyclin A1 protein modification and the activation of caspase-3. Staurosporine 41-54 caspase 3 Homo sapiens 204-213 18638274-4 2008 We demonstrated that epidermal growth factor (EGF) stimulation of fibroblast NR6WT expressing human EGF receptors blocks staurosporine-induced apoptosis by inhibiting the activation of caspase-3. Staurosporine 121-134 caspase 3 Homo sapiens 185-194 18638274-8 2008 These findings indicate that EGF receptor activation provides survival response against staurosporine-induced apoptosis through signal pathways of PI3K and Rac, which then may prevent the activation of caspase-3. Staurosporine 88-101 caspase 3 Homo sapiens 202-211 18189315-7 2008 DHEAS, DHEA, and PGL significantly antagonized effects of staurosporine on both caspase-3 activity and mitochondrial membrane potential. Staurosporine 58-71 caspase 3 Homo sapiens 80-89 17950294-13 2008 In addition, DHA in comparison to AA augmented staurosporine-mediated increase in caspase-3 activity. Staurosporine 47-60 caspase 3 Homo sapiens 82-91 18082144-4 2008 Furthermore, we show three applications using the microarray: (1) detection of autophosphorylation activity of DBtagged human protein kinases and inhibition of their activity by staurosporine, (2) specific cleavage of DBtagged proteins by a virus protease and caspase 3, and (3) detection of a protein-protein interaction between the DBtagged UBE2N and UBE2v1. Staurosporine 178-191 caspase 3 Homo sapiens 260-269 17901567-3 2007 Twenty-four hour incubation with staurosporine (1 microM) enhanced the caspase-3 activity, decreased mitochondrial membrane potential and increased the number of apoptotic cells as visualized by Hoechst staining. Staurosporine 33-46 caspase 3 Homo sapiens 71-80 17901567-4 2007 1,25-Dihydroxyvitamin D3 and PRI-2191 attenuated the staurosporine-induced caspase-3 activity at 5, 50 and 500 nM, whereas PRI-1890 and PRI-1901 were active only at higher concentrations. Staurosporine 53-66 caspase 3 Homo sapiens 75-84 16780893-7 2006 Moreover, it inhibits the cleavage of procaspase-3 mediated by proapoptotic member Bax or apoptosis inductor staurosporine. Staurosporine 109-122 caspase 3 Homo sapiens 38-50 17121821-0 2007 The C-terminal products of cellular prion protein processing, C1 and C2, exert distinct influence on p53-dependent staurosporine-induced caspase-3 activation. Staurosporine 115-128 caspase 3 Homo sapiens 137-146 17121821-7 2007 We show that C1 potentiates staurosporine-induced caspase-3 activation through a p53-dependent mechanism. Staurosporine 28-41 caspase 3 Homo sapiens 50-59 16968671-6 2007 Staurosporine activated apoptotic pathways (i.e. caspase-3 and caspase-9) increasing reactive oxygen species in myoblasts and, to a minor extent, in myotubes. Staurosporine 0-13 caspase 3 Homo sapiens 49-58 17075901-3 2007 Staurosporine-induced cytochrome c release, caspase-3 activation, and production of reactive oxygen species (ROS) were significantly attenuated in S1 cells as compared to V cells and reduced by antioxidants, trolox and GSH-ethyl ester (GSH-EE). Staurosporine 0-13 caspase 3 Homo sapiens 44-53 17075901-5 2007 Mcl-1 was then cleaved to a shortened form in a caspase-3 dependent manner; its release was attenuated far more in S1 than in V cells after staurosporine treatment. Staurosporine 140-153 caspase 3 Homo sapiens 48-57 17093075-7 2006 Moreover, in cultures, the PN donor 3-morpholinosydnonimine (SIN-1) blocked staurosporine-induced caspase-3 activation and its downstream effects including PARP-1 [poly-(ADP-ribose) polymerase-1] cleavage and phosphotidylserine inversion, suggesting that peroxynitrite can inhibit caspase-3-mediated apoptosis. Staurosporine 76-89 caspase 3 Homo sapiens 98-107 17093075-7 2006 Moreover, in cultures, the PN donor 3-morpholinosydnonimine (SIN-1) blocked staurosporine-induced caspase-3 activation and its downstream effects including PARP-1 [poly-(ADP-ribose) polymerase-1] cleavage and phosphotidylserine inversion, suggesting that peroxynitrite can inhibit caspase-3-mediated apoptosis. Staurosporine 76-89 caspase 3 Homo sapiens 281-290 17276981-6 2007 In support of an apoptosis rather than necrosis process, Aph-1 and Pen-2 also lower staurosporine- and etoposide-induced caspase-3 expression and diminished caspase-3 activity and poly(ADP-ribose) polymerase inactivation. Staurosporine 84-97 caspase 3 Homo sapiens 121-130 17276981-8 2007 Furthermore, the Aph-1- and Pen-2-associated reduction of staurosporine-induced caspase-3 activation was fully abolished by p53 deficiency. Staurosporine 58-71 caspase 3 Homo sapiens 80-89 17276981-11 2007 Furthermore, Aph-1- and Pen-2-associated protection against staurosporine-induced caspase-3 activation was not affected by the gamma-secretase inhibitors N-[N-(3,5-difluorophenacetyl)-l-alanyl]-S-phenylglycine t-butyl ester and difluoromethylketone. Staurosporine 60-73 caspase 3 Homo sapiens 82-91 17003475-5 2006 Primary pulmonary endothelial cells internalized human A1AT, which co-localized with and inhibited staurosporine-induced caspase-3 activation. Staurosporine 99-112 caspase 3 Homo sapiens 121-130 17032165-4 2006 IL-18 reduces NK cell self-destruction during NK-targeted cell killing, and in the presence of staurosporin, a potent apoptotic inducer, IL-18 reduces caspase-3 activity. Staurosporine 95-107 caspase 3 Homo sapiens 151-160 16428383-6 2006 Consistent with these data, NEU3 markedly inhibited staurosporine-induced caspase-3 activity and enhanced IL-6-dependent inhibition, which was abolished by LY294002, a PI3K inhibitor. Staurosporine 52-65 caspase 3 Homo sapiens 74-83 16620791-4 2006 Using the activation of caspase-3 as indicator of apoptosis, we found that in two cell lines, Jurkat and Mono-Mac 6, staurosporine and DTT elicited apoptosis with a different pattern: staurosporine acted rapidly and at nanomolar concentrations while DTT acted slowly and at higher concentrations (1mM). Staurosporine 117-130 caspase 3 Homo sapiens 24-33 16620791-4 2006 Using the activation of caspase-3 as indicator of apoptosis, we found that in two cell lines, Jurkat and Mono-Mac 6, staurosporine and DTT elicited apoptosis with a different pattern: staurosporine acted rapidly and at nanomolar concentrations while DTT acted slowly and at higher concentrations (1mM). Staurosporine 184-197 caspase 3 Homo sapiens 24-33 16699130-2 2006 In this study, the authors describe a multiplexed assay for caspase 3 activation, nuclear condensation, and cell viability in a neuronal precursor cell line Ntera-2, injured with staurosporine and etoposide. Staurosporine 179-192 caspase 3 Homo sapiens 60-69 16523241-5 2006 In addition, senescent cells whose FAK expression was downregulated by siRNA showed the increased level of apoptosis by staurosporine treatment via caspase-3 activation but not by hydrogen peroxide treatment. Staurosporine 120-133 caspase 3 Homo sapiens 148-157 16420423-4 2006 Staurosporin application, routinely used to induce apoptosis in mammalian neurons through the activating cleavage of caspase-3, did not result in the appearance of a smaller subunit corresponding to the active caspase in the snail. Staurosporine 0-12 caspase 3 Homo sapiens 117-126 16253284-7 2006 That protection results in a significant reduction of caspase-3 activity induced by staurosporine which by its turn seems to result from a protection observed in the membrane receptor pathway (caspase-8) together with a protection observed in the mitochondrial pathway (caspase-9). Staurosporine 84-97 caspase 3 Homo sapiens 54-63 15660100-5 2005 We found that NO donors ((+/-)-S-nitroso-N-acetylpenicillamine, S-nitrosoglutathione, and NONOates) dose-dependently inhibited caspase-3 and -9 activity induced by STS and camptothecin. Staurosporine 164-167 caspase 3 Homo sapiens 127-143 16199031-3 2005 We compared the apoptotic changes of the nuclear matrix in staurosporine-treated caspase-3-deficient MCF-7 cells transfected with intact CASP-3 gene (MCF-7c3) or an empty vector (MCF-7v) as a control. Staurosporine 59-72 caspase 3 Homo sapiens 81-90 16199031-3 2005 We compared the apoptotic changes of the nuclear matrix in staurosporine-treated caspase-3-deficient MCF-7 cells transfected with intact CASP-3 gene (MCF-7c3) or an empty vector (MCF-7v) as a control. Staurosporine 59-72 caspase 3 Homo sapiens 137-143 16019536-3 2005 We now report that a staurosporine derivative, N-benzoylated staurosporine or PKC412, induces cell death in myeloma cell lines (RPMI8226S, U266, MM1S and MM1R) with loss of mitochondrial membrane potential Delta psi m, caspase 3 and PARP cleavage. Staurosporine 21-34 caspase 3 Homo sapiens 219-228 16019536-3 2005 We now report that a staurosporine derivative, N-benzoylated staurosporine or PKC412, induces cell death in myeloma cell lines (RPMI8226S, U266, MM1S and MM1R) with loss of mitochondrial membrane potential Delta psi m, caspase 3 and PARP cleavage. Staurosporine 61-74 caspase 3 Homo sapiens 219-228 16225760-0 2005 Effect of protein kinase C alpha, caspase-3, and survivin on apoptosis of oral cancer cells induced by staurosporine. Staurosporine 103-116 caspase 3 Homo sapiens 34-43 16225760-9 2005 After treatment with staurosporine, a time-dependent reduction of survivin and an activation of caspase-3 were observed in TSCCa cells. Staurosporine 21-34 caspase 3 Homo sapiens 96-105 16079129-5 2005 We show that overexpression of wild-type PINK1 strongly reduced both basal and staurosporine-induced caspase 3 activity. Staurosporine 79-92 caspase 3 Homo sapiens 101-110 15909127-6 2005 Both H(2)O(2) and staurosporine increased DNA fragmentation and caspase-3 activity and pre-treatment of cells with alpha -lipoic acid and/or alpha -tocopherol failed to prevent stress-induced apoptosis. Staurosporine 18-31 caspase 3 Homo sapiens 64-73 16416674-6 2005 Here, we report c-Abl dependent caspase-3 and caspase-8 activity in response to staurosporine. Staurosporine 80-93 caspase 3 Homo sapiens 32-41 15316934-2 2005 Expression of the WT, but not of the mutant, caspase-3 was associated with increased caspase activity and susceptibility to staurosporine (STS)-induced apoptosis. Staurosporine 124-137 caspase 3 Homo sapiens 45-54 15306200-4 2004 Western blotting showed that both PKC412 (10 microM) and staurosporine (100 nM) cleaved pro-caspase-3 to active forms. Staurosporine 57-70 caspase 3 Homo sapiens 92-101 15140747-5 2004 In KCNA5-transfected COS-7 cells, staurosporine (ST)-mediated increases in caspase-3 activity and the percentage of cells undergoing apoptosis were both enhanced, whereas basal apoptosis (without ST stimulation) was unchanged compared with cells transfected with an empty vector. Staurosporine 34-47 caspase 3 Homo sapiens 75-84 15140747-5 2004 In KCNA5-transfected COS-7 cells, staurosporine (ST)-mediated increases in caspase-3 activity and the percentage of cells undergoing apoptosis were both enhanced, whereas basal apoptosis (without ST stimulation) was unchanged compared with cells transfected with an empty vector. Staurosporine 49-51 caspase 3 Homo sapiens 75-84 15306200-5 2004 An in vitro caspase assay also showed that PKC412 and staurosporine elevated caspase-3 activities. Staurosporine 54-67 caspase 3 Homo sapiens 77-86 15306200-6 2004 Carbobenzoxy-Val-Ala-Asp-fluoromethyl ketone (Z-VAD-FMK), a caspase inhibitor with a broad spectrum, inhibited caspase-3 activities stimulated by PKC412 and staurosporine; however, only PKC412-induced apoptosis, but not staurosporine-induced apoptosis, was prevented by Z-VAD-FMK. Staurosporine 157-170 caspase 3 Homo sapiens 111-120 15306200-6 2004 Carbobenzoxy-Val-Ala-Asp-fluoromethyl ketone (Z-VAD-FMK), a caspase inhibitor with a broad spectrum, inhibited caspase-3 activities stimulated by PKC412 and staurosporine; however, only PKC412-induced apoptosis, but not staurosporine-induced apoptosis, was prevented by Z-VAD-FMK. Staurosporine 220-233 caspase 3 Homo sapiens 111-120 15010857-7 2004 Staurosporine also caused loss of mitochondrial transmembrane potential, release of cytochrome c from mitochondria and activation of caspase-3. Staurosporine 0-13 caspase 3 Homo sapiens 133-142 14963413-4 2004 Prolonged incubation (>2 h) with staurosporine caused a decrease in intracellular pH, which, however, was not required for the progression of the apoptotic process, because inhibitors of proton extrusion pathways, which lowered cytoplasmic pH, failed to inhibit both caspase-3 activation and DNA laddering. Staurosporine 36-49 caspase 3 Homo sapiens 270-279 15093730-5 2004 The activation of the caspase-3 was also inhibited by NMDA, and the neurons were more resistant towards death caused by high concentrations of glutamate and staurosporine. Staurosporine 157-170 caspase 3 Homo sapiens 22-31 12721754-4 2003 RESULTS: A low concentration (</=10 n M) of staurosporine induced G1 arrest of U251MG cells, whereas a high concentration (>/=30 n M) induced G2/M arrest and finally induced apoptosis via a caspase-3-activated pathway from both the G2/M and G1 phases. Staurosporine 47-60 caspase 3 Homo sapiens 196-205 14689449-10 2004 In contrast, ASMase, other lysosomal hydrolases, and caspase 3 remained absent from rafts even after staurosporine treatment. Staurosporine 101-114 caspase 3 Homo sapiens 53-62 14689449-11 2004 The staurosporine-induced concomitant increase of ceramide in the raft fraction and caspase 3 in the cytosol could be mimicked by the addition of exogenous bacterial SMase. Staurosporine 4-17 caspase 3 Homo sapiens 84-93 15033698-11 2003 Caspase 3 activity induced by LPS (24 h) or by staurosporin (6 h) was found to be dramatically downregulated by the G-CSF preincubation protocol. Staurosporine 47-59 caspase 3 Homo sapiens 0-9 14698040-3 2004 Staurosporine (10-100nM) caused the activation of caspase-3. Staurosporine 0-13 caspase 3 Homo sapiens 50-59 14698040-6 2004 VEGF or EGF (10-100ng/mL) added together with staurosporine decreased the activation of caspase-3. Staurosporine 46-59 caspase 3 Homo sapiens 88-97 14570914-6 2003 Proteolytically derived fragments crossreactive with polyclonal anti-band 3 antibody appeared with simultaneous cleavage of poly (ADP-ribose) polymerase and procaspase 3 in staurosporine (STS)-treated HEK293 cells transiently transfected with CDB3 (5). Staurosporine 173-186 caspase 3 Homo sapiens 157-169 14570914-6 2003 Proteolytically derived fragments crossreactive with polyclonal anti-band 3 antibody appeared with simultaneous cleavage of poly (ADP-ribose) polymerase and procaspase 3 in staurosporine (STS)-treated HEK293 cells transiently transfected with CDB3 (5). Staurosporine 188-191 caspase 3 Homo sapiens 157-169 12547651-5 2003 In contrast, treatment with pro-apoptosis agent staurosporine (STS) induced both cytochrome c release and caspase-3 activation after 2h. Staurosporine 48-61 caspase 3 Homo sapiens 106-115 12547651-5 2003 In contrast, treatment with pro-apoptosis agent staurosporine (STS) induced both cytochrome c release and caspase-3 activation after 2h. Staurosporine 63-66 caspase 3 Homo sapiens 106-115 12051665-4 2002 As a positive control for apoptosis, ECV304 cells were treated with staurosporine, which indeed caused significant activation of caspase-3 activity, DNA laddering, and cytochrome c release. Staurosporine 68-81 caspase 3 Homo sapiens 129-138 12598452-13 2003 Caspase-3 activity was detected as early as 3 hours after exposure with staurosporine, peaking at 12 hours of incubation. Staurosporine 72-85 caspase 3 Homo sapiens 0-9 12598452-17 2003 Staurosporine induced apoptosis of endothelial cells involves activation of caspase-3, and could be a useful model to study strategies of cell death inhibition. Staurosporine 0-13 caspase 3 Homo sapiens 76-85 12565805-5 2003 Moreover, the ability of human lens epithelial cells in culture to respond to the apoptosis-inducing agent staurosporin by activation of caspase-3 was investigated. Staurosporine 107-119 caspase 3 Homo sapiens 137-146 12234376-6 2002 Western immunoblot analysis revealed that caspase 3 levels were higher after staurosporine treatment in BRCA1+ cells than in wild type cells, while full length DNA Fragmentation Factor 45 levels were lower in BRCA1+ cells. Staurosporine 77-90 caspase 3 Homo sapiens 42-51 12482880-6 2003 Although PCA inhibited the enzymatic activities of active recombinant caspase-3, caspase-8, and caspase-9 at similar concentrations, PCA exerted weak inhibitory effects on activation of caspase-9 and caspase-3 in staurosporine-treated cells but strongly inhibited caspase-8 activation in FasL-treated cells. Staurosporine 213-226 caspase 3 Homo sapiens 70-79 12581734-0 2003 Staurosporine-induced death of MCF-7 human breast cancer cells: a distinction between caspase-3-dependent steps of apoptosis and the critical lethal lesions. Staurosporine 0-13 caspase 3 Homo sapiens 86-95 12581734-2 2003 Cells were exposed to increasing doses (0.15-1 microM) of staurosporine for periods up to 19 h. Apoptosis was efficiently induced in MCF-7c3 cells, as demonstrated by cytochrome c release, processing of procaspase-3, procaspase-8, and Bid, increase in caspase-3-like DEVDase activity, cleavage of the enzyme poly(ADP-ribose) polymerase, DNA fragmentation, changes in nuclear morphology, and TUNEL assay and flow cytometry. Staurosporine 58-71 caspase 3 Homo sapiens 203-215 12581734-2 2003 Cells were exposed to increasing doses (0.15-1 microM) of staurosporine for periods up to 19 h. Apoptosis was efficiently induced in MCF-7c3 cells, as demonstrated by cytochrome c release, processing of procaspase-3, procaspase-8, and Bid, increase in caspase-3-like DEVDase activity, cleavage of the enzyme poly(ADP-ribose) polymerase, DNA fragmentation, changes in nuclear morphology, and TUNEL assay and flow cytometry. Staurosporine 58-71 caspase 3 Homo sapiens 206-215 12581734-3 2003 For all of these measures except cytochrome c release, little or no activity was detected in MCF-7v cells, confirming that caspase-3 is essential for efficient induction of apoptosis by staurosporine, but not for mitochondrial steps that occur earlier in the pathway. Staurosporine 186-199 caspase 3 Homo sapiens 123-132 12542519-5 2003 Staurosporine, a potent inhibitor of phospholipid Ca2+-dependent protein kinase, increased externalized phosphatidylserine levels on SZ95 sebocytes, detected by annexin V/propidium iodide flow cytometry, as early as after 1 h, whereas dose-dependent reduction of bcl-2 mRNA and protein expression, enhanced DNA fragmentation, and increased caspase 3 levels, detected by caspase 3 inhibitor/propidium iodide flow cytometry, were found after 6 h of treatment. Staurosporine 0-13 caspase 3 Homo sapiens 340-349 12542519-5 2003 Staurosporine, a potent inhibitor of phospholipid Ca2+-dependent protein kinase, increased externalized phosphatidylserine levels on SZ95 sebocytes, detected by annexin V/propidium iodide flow cytometry, as early as after 1 h, whereas dose-dependent reduction of bcl-2 mRNA and protein expression, enhanced DNA fragmentation, and increased caspase 3 levels, detected by caspase 3 inhibitor/propidium iodide flow cytometry, were found after 6 h of treatment. Staurosporine 0-13 caspase 3 Homo sapiens 370-379 12490620-7 2003 The AQZs inhibit endogenous caspase-3 activity toward a cell permeable, exogenously added substrate in staurosporine-treated SH-SY5Y cells. Staurosporine 103-116 caspase 3 Homo sapiens 28-37 12443983-0 2002 Citicoline increases glutathione redox ratio and reduces caspase-3 activation and cell death in staurosporine-treated SH-SY5Y human neuroblastoma cells. Staurosporine 96-109 caspase 3 Homo sapiens 57-66 12443983-7 2002 These findings demonstrate that citicoline affects the staurosporine-induced apoptosis cell-signalling pathway by interacting with the glutathione system and by inhibiting caspase-3 in SH-SY5Y human neuroblastoma cells. Staurosporine 55-68 caspase 3 Homo sapiens 172-181 12373608-4 2002 Staurosporine induced DEVDase activity in T47D cells suggesting the involvement of caspase-3 and/or caspase-7, yet there was no DEVDase activity in MCF-7 cells, probably ruling out the involvement caspase-7. Staurosporine 0-13 caspase 3 Homo sapiens 83-92 12154052-10 2002 Both DNAS1L3-mediated and staurosporine-induced internucleosomal DNA fragmentation were Ca(2+) dependent, but only the DNAS1L3-mediated DNA cleavage was blocked by expression of a caspase-3-resistant mutant of poly(ADP-ribose) polymerase-1. Staurosporine 26-39 caspase 3 Homo sapiens 180-189 11943195-4 2002 In response to apoptotic stimuli such as staurosporine or hyperosmotic stress, caspase-3 activity was significantly greater in the N63-82Q cells compared to the N63-18Q cells. Staurosporine 41-54 caspase 3 Homo sapiens 79-88 12032672-6 2002 In contrast, staurosporine-induced apoptosis in these cells was accompanied by proteolytic cleavage of pro-caspase-3 and induction of caspase-3 enzymatic activity. Staurosporine 13-26 caspase 3 Homo sapiens 103-116 12032672-6 2002 In contrast, staurosporine-induced apoptosis in these cells was accompanied by proteolytic cleavage of pro-caspase-3 and induction of caspase-3 enzymatic activity. Staurosporine 13-26 caspase 3 Homo sapiens 107-116 12065637-5 2002 In SH/tTG cells, osmotic stress and staurosporine treatments resulted in significantly greater caspase-3 activation and apoptotic nuclear changes then in SH/pcDNA or SH/C277S cells. Staurosporine 36-49 caspase 3 Homo sapiens 95-104 11495916-5 2001 Heat shock and staurosporine treatments increased nuclear GSK-3 beta prior to activation of caspase-9 and caspase-3, and this nuclear accumulation of GSK-3 beta was unaltered by pretreatment with a general caspase inhibitor. Staurosporine 15-28 caspase 3 Homo sapiens 106-115 11479289-4 2001 We found that CaMKLK is a substrate for caspase-3 and -8, both in vitro and in NG108 cells during staurosporine- and serum withdrawal-induced apoptosis. Staurosporine 98-111 caspase 3 Homo sapiens 40-56 11570504-12 2001 Pretreatment of staurosporine-induced Jurkat cells with FAM-VAD-FMK inhibited affinity labeling of caspase-3, -6, and -7, blocked caspase-specific cell staining, and led to the inhibition of apoptosis. Staurosporine 16-29 caspase 3 Homo sapiens 99-120 11463339-8 2001 It was also able to monitor caspase-3 activation in cells provoked into apoptosis by staurosporine (1 or 2 microM). Staurosporine 85-98 caspase 3 Homo sapiens 28-37 11426445-1 2001 Exposure of rat hippocampal neurons or human D283 medulloblastoma cells to the apoptosis-inducing kinase inhibitor staurosporine induced rapid cytochrome c release from mitochondria and activation of the executioner caspase-3. Staurosporine 115-128 caspase 3 Homo sapiens 216-225 11058115-3 2000 We report here that caspase-3 and calpain can form p140 from p261 in vitro and in vivo and that during early stages of apoptosis induced in Jurkat cells by staurosporine or anti-Fas-activating antibody, p261 is cleaved into p140 by caspase-3. Staurosporine 156-169 caspase 3 Homo sapiens 20-29 11162250-4 2001 Staurosporine-induced Tau cleavage was blocked by 20 microM z-Asp-Glu-Val-Asp-chloromethylketone, a caspase-3 inhibitor, and in vitro, Tau was selectively cleaved by caspase-3 or calpain, a calcium-activated protease, but not by caspases-1, -8, or -9. Staurosporine 0-13 caspase 3 Homo sapiens 100-109 11162250-4 2001 Staurosporine-induced Tau cleavage was blocked by 20 microM z-Asp-Glu-Val-Asp-chloromethylketone, a caspase-3 inhibitor, and in vitro, Tau was selectively cleaved by caspase-3 or calpain, a calcium-activated protease, but not by caspases-1, -8, or -9. Staurosporine 0-13 caspase 3 Homo sapiens 166-175 11296547-3 2001 We found that staurosporine-mediated chondrocyte death depended on the concentration and time of incubation, and coincided with increased Bax:Bcl-X mRNA expression, cytochrome C release, and activation of caspase-3. Staurosporine 14-27 caspase 3 Homo sapiens 205-214 11296547-5 2001 Cell protection coincided with inhibition of the staurosporine-mediated induction of caspase-3 activation. Staurosporine 49-62 caspase 3 Homo sapiens 85-94 11146107-0 2000 Staurosporine- and H-7-induced cell death in SH-SY5Y neuroblastoma cells is associated with caspase-2 and caspase-3 activation, but not with activation of the FAS/FAS-L-caspase-8 signaling pathway. Staurosporine 0-13 caspase 3 Homo sapiens 106-115 11146107-3 2000 The process is inhibited with DEVD-fmk, a potent caspase-3 (and caspase-8) inhibitor, thus indicating that staurosporine- and H-7-induced cell death in SH-SY5Y is mediated by caspase activation. Staurosporine 107-120 caspase 3 Homo sapiens 49-58 11187905-6 2000 However, activation of both caspase-8 and caspase-3 was achieved in BR97 cells treated with staurosporine. Staurosporine 92-105 caspase 3 Homo sapiens 42-51 11154867-2 2001 It has been demonstrated that the protease caspase-3, a downstream molecule of the CD95 pathway, is activated in UV-exposed HaCaT cells, and that the DNA-dependent protein kinase catalytic subunit (DNA-PKcs) is cleaved by interleukin-1beta converting enzyme (ICE)-like protease during apoptosis induced by X-rays, staurosporine and etoposide. Staurosporine 314-327 caspase 3 Homo sapiens 43-52 10998058-4 2000 The absence of HsRad51 cleavage in staurosporine-treated apoptotic MCF-7 cells, which lack caspase-3, indicates that caspase-3 is essential for HsRad51 cleavage in vivo. Staurosporine 35-48 caspase 3 Homo sapiens 91-100 10998058-4 2000 The absence of HsRad51 cleavage in staurosporine-treated apoptotic MCF-7 cells, which lack caspase-3, indicates that caspase-3 is essential for HsRad51 cleavage in vivo. Staurosporine 35-48 caspase 3 Homo sapiens 117-126 10933864-5 2000 Using the assay we detected 6.6 ng active caspase 3 per 10(6) apoptotic staurosporine-treated Jurkat cells. Staurosporine 72-85 caspase 3 Homo sapiens 42-51 10934034-8 2000 In the presence of z-DEVD-FMK or Ac-YVAD-CMK, caspase-3 was processed to both the p17 and p20 fragments in staurosporine-treated cells, but only to p20 fragment in the presence of z-VAD-FMK. Staurosporine 107-120 caspase 3 Homo sapiens 46-55 10934034-10 2000 In addition, caspase-3 null MCF-7 cells also undergo pre-apoptotic nuclear change when treated with staurosporine in the presence of caspase inhibitors, indicating that caspase-3 is not required for the early nuclear morphological change in cells undergoing apoptosis. Staurosporine 100-113 caspase 3 Homo sapiens 13-22 10713065-3 2000 Staurosporine treatment caused time- and concentration-dependent increases in the activities of caspase-3 and caspase-9 but not caspase-1, increased proteolysis of poly(ADP-ribose) polymerase, and induced morphological changes consistent with apoptosis. Staurosporine 0-13 caspase 3 Homo sapiens 96-105 10762713-4 2000 Cells exposed to staurosporine (0.1 microM) for 30 min showed an increase in caspase 3-activity and by 1 h an increase in PARP 116-kDa band and an 85-kDa cleavage product, which further increased in density with time after treatment. Staurosporine 17-30 caspase 3 Homo sapiens 77-86 10955723-6 2000 The staurosporine-induced DNA ladder formation was accompanied by an increase in caspase-3 activity in both lines which, however, was considerably lower in Jurkat JM cells after gemcitabine or cycloheximide exposure. Staurosporine 4-17 caspase 3 Homo sapiens 81-90 10734071-0 2000 Caspase-8 activation and bid cleavage contribute to MCF7 cellular execution in a caspase-3-dependent manner during staurosporine-mediated apoptosis. Staurosporine 115-128 caspase 3 Homo sapiens 81-90 10708590-7 2000 Western analysis showed cleavage of caspase-9, an initiator caspase, of caspase-3, an executioner caspase, and of a caspase substrate, poly-(ADP-ribose)-polymerase (PARP) in staurosporine-treated cells. Staurosporine 174-187 caspase 3 Homo sapiens 72-81 10713065-4 2000 Overexpression of glycogen synthase kinase-3beta to levels 3.5 times that in control cells did not alter basal indices of apoptosis but potentiated staurosporine-induced activation of caspase-3, caspase-9, proteolysis of poly(ADP-ribose) polymerase, and morphological changes indicative of apoptosis. Staurosporine 148-161 caspase 3 Homo sapiens 184-193 10713065-5 2000 Inhibition of glycogen synthase kinase-3beta by lithium attenuated the enhanced staurosporine-induced activation of caspase-3 in cells overexpressing glycogen synthase kinase-3beta. Staurosporine 80-93 caspase 3 Homo sapiens 116-125 10514472-6 1999 Upon staurosporine treatment, cytochrome c was released concomitantly with activation of caspase 3 and loss of mitochondrial membrane potential (Deltapsi(m)). Staurosporine 5-18 caspase 3 Homo sapiens 89-98 10663630-7 2000 Caspase-3 activity increased more than 20-fold in cells treated with actinomycin D, staurosporine, or cisplatin but increased less than fivefold in ACNU-treated cells. Staurosporine 84-97 caspase 3 Homo sapiens 0-9 10582585-0 1999 Staurosporine-induced activation of caspase-3 is potentiated by presenilin 1 familial Alzheimer"s disease mutations in human neuroglioma cells. Staurosporine 0-13 caspase 3 Homo sapiens 36-45 10582585-5 1999 Staurosporine treatment of these cells resulted in increased cell death and up to a 10-fold increase in caspase-3 activation in mutant versus wt PS1-expressing cell lines. Staurosporine 0-13 caspase 3 Homo sapiens 104-113 10679755-3 2000 RESULTS: The LNCaP and TsuPr(1) lines exhibited quintessential apoptotic features in response to the pleiotropic apoptotic inducer staurosporine (STS): rapid cytochrome c translocation to the cytosol, proteolytic processing and catalytic activation of caspase-3 and -7, proteolytic inactivation of the death substrates DNA fragmentation factor (DFF) and poly-ADP-ribose polymerase (PARP), and TUNEL-positive polyfragmented nuclei. Staurosporine 131-144 caspase 3 Homo sapiens 252-268 10567423-7 1999 Tumor necrosis factor-alpha- or staurosporine-induced apoptosis in caspase-3-deficient MCF-7 cells failed to demonstrate cleavage of IP(3)R1. Staurosporine 32-45 caspase 3 Homo sapiens 67-76 10438458-7 1999 Expression of this mutant PARP increased the rate of staurosporine and tumor necrosis factor-alpha-induced apoptosis, at least in part by reducing the time interval required for the onset of caspase-3 activation and internucleosomal DNA fragmentation, as well as the generation of 50-kilobase pair DNA breaks, thought to be associated with early chromatin unfolding. Staurosporine 53-66 caspase 3 Homo sapiens 191-200 10497198-10 1999 Furthermore, caspase-7 activation was concommitant with PARP cleavage in the caspase-3-deficient cell line MCF-7 in response to staurosporine treatment. Staurosporine 128-141 caspase 3 Homo sapiens 77-86 10486259-6 1999 Finally, we show that staurosporine-mediated caspase-3 activation is interrupted by maitotoxin pretreatment. Staurosporine 22-35 caspase 3 Homo sapiens 45-54 10477698-3 1999 The apoptotic pathway distal to the DISC is intact because ceramide analogs, staurosporine, and granzyme B activate caspase-3 and induce apoptosis. Staurosporine 77-90 caspase 3 Homo sapiens 116-125 10409669-10 1999 However, transient transfection of caspase-3 into MCF-7 cells restores Bcl-2 cleavage after staurosporine treatment. Staurosporine 92-105 caspase 3 Homo sapiens 35-44 10728572-5 1999 In contrast, staurosporin treated PBL showed a decrease in delta psi m with cytochrome-c release and a clear caspase 3 activation. Staurosporine 13-25 caspase 3 Homo sapiens 109-118 10375546-9 1999 In Cos-7 cells, caspase-1 and caspase-3 substrates were cleaved upon induction of apoptosis with staurosporine, a protein-kinase inhibitor, whereas caspase-3 but not caspase-1 substrate was cleaved upon treatment of cells with the DNA-damaging agent mitomycin c. Staurosporine 97-110 caspase 3 Homo sapiens 30-39 9949201-7 1999 Treatment with apoptosis inhibitors rescued FRDA but not control fibroblasts from oxidant stress, and staurosporine-induced caspase 3 activity was higher in FRDA fibroblasts, consistent with the possibility that an apoptotic step upstream of caspase 3 is activated in FRDA fibroblasts. Staurosporine 102-115 caspase 3 Homo sapiens 124-133 9949201-7 1999 Treatment with apoptosis inhibitors rescued FRDA but not control fibroblasts from oxidant stress, and staurosporine-induced caspase 3 activity was higher in FRDA fibroblasts, consistent with the possibility that an apoptotic step upstream of caspase 3 is activated in FRDA fibroblasts. Staurosporine 102-115 caspase 3 Homo sapiens 242-251 9927051-2 1999 The inhibitor of phosphomevalonate decarboxylase, sodium phenylacetate, and the protein kinase inhibitor staurosporine induced (a) release of cytochrome c from the mitochondria to the cytosol; (b) reduction in mitochondrial transmembrane potential; (c) proteolytic processing of caspase-3 and -7 but not -2; (d) cleavage of the DEVD substrate and the death substrates poly(ADP-ribose) polymerase and DNA fragmentation factor; and (e) apoptosis. Staurosporine 105-118 caspase 3 Homo sapiens 279-295 10205158-5 1999 Induction of apoptosis with staurosporine led to the activation of mitochondrial pro-caspase-3 and its dissociation from the Hsps which were released from mitochondria. Staurosporine 28-41 caspase 3 Homo sapiens 81-94 10037154-4 1999 Staurosporine but not wortmannin caused the intracellular proteolytic processing of pro-caspase-3 and this event was transiently inhibited by EGF. Staurosporine 0-13 caspase 3 Homo sapiens 84-97 9799125-9 1998 Taken together, these results show that ginsenoside Rh2 and staurosporine increase caspase-3 activity, which in turn directly cleaves p21WAF1/CIP1 during the early stages of apoptosis. Staurosporine 60-73 caspase 3 Homo sapiens 83-92 9624143-3 1998 Tumor necrosis factor- or staurosporine-induced apoptosis of caspase-3-deficient MCF-7 cells resulted in cleavage of the death substrates PARP, Rb, PAK2, DNA-PKcs, gelsolin, and DFF-45, but not alpha-fodrin. Staurosporine 26-39 caspase 3 Homo sapiens 61-70 9624143-6 1998 In addition, tumor necrosis factor- or staurosporine-induced apoptosis of MCF-7 cells stably expressing pro-caspase-3 also resulted in alpha-fodrin cleavage. Staurosporine 39-52 caspase 3 Homo sapiens 104-117 9515027-0 1998 Staurosporine-induced apoptosis in cardiomyocytes: A potential role of caspase-3. Staurosporine 0-13 caspase 3 Homo sapiens 71-80 10200498-1 1998 Treatment of HL-60 cells with staurosporine (STS) induced mitochondrial cytochrome c efflux into the cytosol, which was followed by caspase-3 activation and apoptosis. Staurosporine 30-43 caspase 3 Homo sapiens 132-141 10200498-1 1998 Treatment of HL-60 cells with staurosporine (STS) induced mitochondrial cytochrome c efflux into the cytosol, which was followed by caspase-3 activation and apoptosis. Staurosporine 45-48 caspase 3 Homo sapiens 132-141 9515027-12 1998 Moreover, the appearance of the 17-kD subunit of active caspase-3 in staurosporine-treated myocytes was demonstrated by immunocytochemical analysis. Staurosporine 69-82 caspase 3 Homo sapiens 56-65 9515027-15 1998 Our results suggest that staurosporine-induced cardiac myocyte apoptosis involves activation of caspases, mainly caspase-3, but not activation of the SAPK signaling pathway. Staurosporine 25-38 caspase 3 Homo sapiens 113-122 9368003-8 1997 In addition, PRK2 is cleaved rapidly during Fas- and staurosporine-induced apoptosis in vivo by caspase-3 or a closely related caspase. Staurosporine 53-66 caspase 3 Homo sapiens 96-105 9288776-5 1997 Synergy between butyrate and staurosporine was due to the presence of a factor in the cytosol of butyrate-primed cells which enhanced over 7-fold the activation of caspase-3 induced by the addition of cytochrome c and dATP to isolated cytosol. Staurosporine 29-42 caspase 3 Homo sapiens 164-173 9166725-4 1997 Furthermore, the hydrolysis of the CPP32 substrate acetyl-DEVD-7-amido-4-methylcoumarin was detected as early as 3 h and became maximal at 6 h after staurosporine challenge, suggesting a delayed and sustained period of CPP32-like activation. Staurosporine 149-162 caspase 3 Homo sapiens 35-40 9166725-4 1997 Furthermore, the hydrolysis of the CPP32 substrate acetyl-DEVD-7-amido-4-methylcoumarin was detected as early as 3 h and became maximal at 6 h after staurosporine challenge, suggesting a delayed and sustained period of CPP32-like activation. Staurosporine 149-162 caspase 3 Homo sapiens 219-224 9166725-7 1997 Following staurosporine challenge there was a condensing of CPP32 immunofluorescence from the cytoplasm to a region adjacent to the plasma membrane. Staurosporine 10-23 caspase 3 Homo sapiens 60-65 16465208-6 1997 Here, we demonstrate that CPP32/Yama/Apopain, a member of the ICE/Ced-3 gene family, is processed during staurosporine-induced apoptosis in HeLa cells and that concomitant with CPP32 activation, two other proteins, poly (ADP-ribose) polymerase (PARP) and the U1-70 K small ribonucleoprotein, also undergo proteolysis. Staurosporine 105-118 caspase 3 Homo sapiens 37-44 9120020-7 1997 Next, we showed that apoptosis induced by TNF and staurosporine were blocked by z-DEVD-CH2F, an inhibitor of CPP32-like cysteine protease, suggesting the involvement of CPP32-like protease in both apoptosis signaling pathways. Staurosporine 50-63 caspase 3 Homo sapiens 109-114 9120020-7 1997 Next, we showed that apoptosis induced by TNF and staurosporine were blocked by z-DEVD-CH2F, an inhibitor of CPP32-like cysteine protease, suggesting the involvement of CPP32-like protease in both apoptosis signaling pathways. Staurosporine 50-63 caspase 3 Homo sapiens 169-174 16465208-6 1997 Here, we demonstrate that CPP32/Yama/Apopain, a member of the ICE/Ced-3 gene family, is processed during staurosporine-induced apoptosis in HeLa cells and that concomitant with CPP32 activation, two other proteins, poly (ADP-ribose) polymerase (PARP) and the U1-70 K small ribonucleoprotein, also undergo proteolysis. Staurosporine 105-118 caspase 3 Homo sapiens 32-36 16465208-0 1997 Bcl-2 prevents activation of CPP32 cysteine protease and cleavage of poly (ADP-ribose) polymerase and U1-70 kD proteins in staurosporine-mediated apoptosis. Staurosporine 123-136 caspase 3 Homo sapiens 29-34 16465208-6 1997 Here, we demonstrate that CPP32/Yama/Apopain, a member of the ICE/Ced-3 gene family, is processed during staurosporine-induced apoptosis in HeLa cells and that concomitant with CPP32 activation, two other proteins, poly (ADP-ribose) polymerase (PARP) and the U1-70 K small ribonucleoprotein, also undergo proteolysis. Staurosporine 105-118 caspase 3 Homo sapiens 26-31 16465208-6 1997 Here, we demonstrate that CPP32/Yama/Apopain, a member of the ICE/Ced-3 gene family, is processed during staurosporine-induced apoptosis in HeLa cells and that concomitant with CPP32 activation, two other proteins, poly (ADP-ribose) polymerase (PARP) and the U1-70 K small ribonucleoprotein, also undergo proteolysis. Staurosporine 105-118 caspase 3 Homo sapiens 177-182 34878630-8 2022 Caspase 3/7 activity was suppressed after administration of staurosporine in MEN1 knocked down HPSC2.2 and MEN1-/- MEFs as well. Staurosporine 60-73 caspase 3 Homo sapiens 0-11 35186954-5 2021 Drug-induced apoptosis was analyzed after exposure to staurosporine by caspase 3/7 activity and by annexin-V/7-actinomycin D (7-AAD) staining, followed by flow cytometry. Staurosporine 54-67 caspase 3 Homo sapiens 71-82 35186954-12 2021 NPPB and NFA also suppressed staurosporine-induced caspase 3/7 activation, while DCPIB and Tamoxifen showed cytotoxic effects per se. Staurosporine 29-42 caspase 3 Homo sapiens 51-62 32714930-7 2020 At the same time, staurosporine, nifedipine, and tunicamycin elicited an intermediate activation of caspase-3. Staurosporine 18-31 caspase 3 Homo sapiens 100-109 32982774-7 2020 Collectively, these results suggest that the cellular redox status may be one of the critical factors of the apoptotic pathway leading to caspase-3 activation by staurosporine. Staurosporine 162-175 caspase 3 Homo sapiens 138-147 32982774-0 2020 Role of Glutathione Depletion and Reactive Oxygen Species Generation on Caspase-3 Activation: A Study With the Kinase Inhibitor Staurosporine. Staurosporine 128-141 caspase 3 Homo sapiens 72-81 32982774-5 2020 The introduction of staurosporine significantly decreased the concentration of cellular glutathione and increased the presence of ROS after 3 h. These findings were concurrent with the activation of caspase-3. Staurosporine 20-33 caspase 3 Homo sapiens 199-208 32214021-8 2020 Staurosporin increased caspase-3/7 activity (689%) and decreased cell survival by 32%. Staurosporine 0-12 caspase 3 Homo sapiens 23-32 32032391-8 2020 Less active caspase-8 and caspase-9, and consequently less active caspase-3, was observed in infected compared to uninfected staurosporine-exposed cells. Staurosporine 125-138 caspase 3 Homo sapiens 66-75