PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 33168626-7 2021 In the current study, we found that caspase activation by staurosporine- and actinomycin D-induced PARP1 auto-poly(ADP-ribosyl)ation and fragmentation, generating poly(ADP-ribosyl)ated 89-kDa and 24-kDa PARP1 fragments. Staurosporine 58-71 poly(ADP-ribose) polymerase 1 Homo sapiens 99-104 33168626-7 2021 In the current study, we found that caspase activation by staurosporine- and actinomycin D-induced PARP1 auto-poly(ADP-ribosyl)ation and fragmentation, generating poly(ADP-ribosyl)ated 89-kDa and 24-kDa PARP1 fragments. Staurosporine 58-71 poly(ADP-ribose) polymerase 1 Homo sapiens 203-208 25010987-7 2014 Functionally, an elevation in caspase-2 level by HuR knockdown correlated with an increased sensitivity of cells to apoptosis induced by staurosporine- and pore-forming toxins as implicated by their significant accumulation in the sub G1 phase and an increase in caspase-2, -3 and poly ADP-ribose polymerase cleavage, respectively. Staurosporine 137-150 poly(ADP-ribose) polymerase 1 Homo sapiens 263-307 25680752-2 2015 In this study, we observed that STS treatment induced drastic morphologic changes, such as elongation of a very large number of nonbranched, actin-based long cell protrusions that reached up to 30 microm in an hour without caspase activation or PARP cleavage in fibroblasts and epithelial cells. Staurosporine 32-35 poly(ADP-ribose) polymerase 1 Homo sapiens 245-249 25481756-4 2015 In this study, we demonstrated that CCHFV infection suppresses cleavage of poly(ADP-ribose) polymerase (PARP), triggered by staurosporine early post-infection. Staurosporine 124-137 poly(ADP-ribose) polymerase 1 Homo sapiens 104-108 19216720-8 2009 Meanwhile, the use of 3-aminobenzamide, a PARP-1 inhibitor known to prevent AIF (apoptosis-inducing factor) release, significantly decreases staurosporine-induced death in these p53mt carcinoma cells, suggesting a preferential implication of caspase-independent apoptosis. Staurosporine 141-154 poly(ADP-ribose) polymerase 1 Homo sapiens 42-48 23097134-4 2013 Using human neuroblastoma cell lines, SK-N-MC, SH-SY5Y, and SK-N-SH, we show that non-toxic dose (2 mM) of VPA enhanced staurosporine (STS)-induced cell death as assessed by MTT assay, PARP cleavage, hypodiploidy, and caspase 3 activity. Staurosporine 120-133 poly(ADP-ribose) polymerase 1 Homo sapiens 185-189 23097134-4 2013 Using human neuroblastoma cell lines, SK-N-MC, SH-SY5Y, and SK-N-SH, we show that non-toxic dose (2 mM) of VPA enhanced staurosporine (STS)-induced cell death as assessed by MTT assay, PARP cleavage, hypodiploidy, and caspase 3 activity. Staurosporine 135-138 poly(ADP-ribose) polymerase 1 Homo sapiens 185-189 24421315-7 2014 PDGF-C prevented staurosporine-induced macrophage apoptosis by inhibiting the activation of caspase-3, -7, -8, and -9 and cleavage of poly(ADP-ribose) polymerase. Staurosporine 17-30 poly(ADP-ribose) polymerase 1 Homo sapiens 134-161 23333591-3 2013 STS treatment, without T. gondii infection, reduced the viability of THP-1 cells in proportion to STS concentration and triggered many cellular death events such as caspase-3 and -9 activation, Bax translocation, cytochrome c release from host cell mitochondria into cytosol, and PARP cleavage in the host cell. Staurosporine 0-3 poly(ADP-ribose) polymerase 1 Homo sapiens 280-284 21843636-5 2011 While PPARbeta/delta-dependent attenuation of staurosporine- or UVB-induced poly (ADP-ribose) polymerase (PARP) cleavage was not observed, PPARbeta/delta- and PPARgamma-dependent repression of UVB-induced expression and secretion of inflammatory cytokines was found in HaCaT keratinocytes over-expressing PPARbeta/delta or PPARgamma. Staurosporine 46-59 poly(ADP-ribose) polymerase 1 Homo sapiens 106-110 19609857-4 2009 G292 cells revealed the expression of cleaved PARP after incubation with staurosporine for 3 hours. Staurosporine 73-86 poly(ADP-ribose) polymerase 1 Homo sapiens 46-50 17093075-7 2006 Moreover, in cultures, the PN donor 3-morpholinosydnonimine (SIN-1) blocked staurosporine-induced caspase-3 activation and its downstream effects including PARP-1 [poly-(ADP-ribose) polymerase-1] cleavage and phosphotidylserine inversion, suggesting that peroxynitrite can inhibit caspase-3-mediated apoptosis. Staurosporine 76-89 poly(ADP-ribose) polymerase 1 Homo sapiens 164-194 18534571-3 2008 Staurosporine increased caspase 3-like activity, DNA fragmentation, PARP cleavage, and the number of TUNEL positive cells consistent with the induction of apoptosis. Staurosporine 0-13 poly(ADP-ribose) polymerase 1 Homo sapiens 68-72 18339380-10 2009 PARP proteins (75 kDa, 63 kDa, and 60 kDa) were evaluated after inducing apoptosis by hydrogen peroxide and staurosporine exposure. Staurosporine 108-121 poly(ADP-ribose) polymerase 1 Homo sapiens 0-4 18594935-5 2008 However, upon treatment with staurosporine, BRAt cells showed increased levels of active caspase-3 and increased cleavage of caspase-3 substrates, PARP and DFF45. Staurosporine 29-42 poly(ADP-ribose) polymerase 1 Homo sapiens 147-151 12443983-6 2002 Finally, citicoline also reduces the expression levels of active caspase-3 and specific PARP-cleaved products of 89 kDa resulting from staurosporine exposure when citicoline is added to the culture medium 24 h before staurosporine. Staurosporine 135-148 poly(ADP-ribose) polymerase 1 Homo sapiens 88-92 14570914-6 2003 Proteolytically derived fragments crossreactive with polyclonal anti-band 3 antibody appeared with simultaneous cleavage of poly (ADP-ribose) polymerase and procaspase 3 in staurosporine (STS)-treated HEK293 cells transiently transfected with CDB3 (5). Staurosporine 173-186 poly(ADP-ribose) polymerase 1 Homo sapiens 124-152 14570914-6 2003 Proteolytically derived fragments crossreactive with polyclonal anti-band 3 antibody appeared with simultaneous cleavage of poly (ADP-ribose) polymerase and procaspase 3 in staurosporine (STS)-treated HEK293 cells transiently transfected with CDB3 (5). Staurosporine 188-191 poly(ADP-ribose) polymerase 1 Homo sapiens 124-152 12581734-2 2003 Cells were exposed to increasing doses (0.15-1 microM) of staurosporine for periods up to 19 h. Apoptosis was efficiently induced in MCF-7c3 cells, as demonstrated by cytochrome c release, processing of procaspase-3, procaspase-8, and Bid, increase in caspase-3-like DEVDase activity, cleavage of the enzyme poly(ADP-ribose) polymerase, DNA fragmentation, changes in nuclear morphology, and TUNEL assay and flow cytometry. Staurosporine 58-71 poly(ADP-ribose) polymerase 1 Homo sapiens 308-335 12603826-3 2003 Staurosporine, used as a positive control for apoptosis, caused Poly(ADP-ribose)-polymerase (PARP) cleavage and nuclear fragmentation, but no PARP cleavage and little fragmentation were seen after OGD. Staurosporine 0-13 poly(ADP-ribose) polymerase 1 Homo sapiens 64-91 12603826-3 2003 Staurosporine, used as a positive control for apoptosis, caused Poly(ADP-ribose)-polymerase (PARP) cleavage and nuclear fragmentation, but no PARP cleavage and little fragmentation were seen after OGD. Staurosporine 0-13 poly(ADP-ribose) polymerase 1 Homo sapiens 93-97 12443983-6 2002 Finally, citicoline also reduces the expression levels of active caspase-3 and specific PARP-cleaved products of 89 kDa resulting from staurosporine exposure when citicoline is added to the culture medium 24 h before staurosporine. Staurosporine 217-230 poly(ADP-ribose) polymerase 1 Homo sapiens 88-92 12373608-6 2002 Caspase dependent PARP cleavage was detected in MCF-7 cells at 3 h, whereas only partial PARP cleavage was detected in T47D cells and then only after 24 h. Moreover, staurosporine led to cytochrome c release at 2 h in MCF-7 cells and 6 h in T47D cells. Staurosporine 166-179 poly(ADP-ribose) polymerase 1 Homo sapiens 18-22 12373608-6 2002 Caspase dependent PARP cleavage was detected in MCF-7 cells at 3 h, whereas only partial PARP cleavage was detected in T47D cells and then only after 24 h. Moreover, staurosporine led to cytochrome c release at 2 h in MCF-7 cells and 6 h in T47D cells. Staurosporine 166-179 poly(ADP-ribose) polymerase 1 Homo sapiens 89-93 11853409-6 2002 SV and staurosporine-induced cell death was delayed in fibroblasts lacking PARP-1 activity, suggesting that PARP-1 activation contributes to apoptotic cell death induced by these stimuli. Staurosporine 7-20 poly(ADP-ribose) polymerase 1 Homo sapiens 75-81 12154052-10 2002 Both DNAS1L3-mediated and staurosporine-induced internucleosomal DNA fragmentation were Ca(2+) dependent, but only the DNAS1L3-mediated DNA cleavage was blocked by expression of a caspase-3-resistant mutant of poly(ADP-ribose) polymerase-1. Staurosporine 26-39 poly(ADP-ribose) polymerase 1 Homo sapiens 210-239 11853409-6 2002 SV and staurosporine-induced cell death was delayed in fibroblasts lacking PARP-1 activity, suggesting that PARP-1 activation contributes to apoptotic cell death induced by these stimuli. Staurosporine 7-20 poly(ADP-ribose) polymerase 1 Homo sapiens 108-114 10713065-3 2000 Staurosporine treatment caused time- and concentration-dependent increases in the activities of caspase-3 and caspase-9 but not caspase-1, increased proteolysis of poly(ADP-ribose) polymerase, and induced morphological changes consistent with apoptosis. Staurosporine 0-13 poly(ADP-ribose) polymerase 1 Homo sapiens 164-191 10762713-4 2000 Cells exposed to staurosporine (0.1 microM) for 30 min showed an increase in caspase 3-activity and by 1 h an increase in PARP 116-kDa band and an 85-kDa cleavage product, which further increased in density with time after treatment. Staurosporine 17-30 poly(ADP-ribose) polymerase 1 Homo sapiens 122-126 10708590-7 2000 Western analysis showed cleavage of caspase-9, an initiator caspase, of caspase-3, an executioner caspase, and of a caspase substrate, poly-(ADP-ribose)-polymerase (PARP) in staurosporine-treated cells. Staurosporine 174-187 poly(ADP-ribose) polymerase 1 Homo sapiens 135-163 10708590-7 2000 Western analysis showed cleavage of caspase-9, an initiator caspase, of caspase-3, an executioner caspase, and of a caspase substrate, poly-(ADP-ribose)-polymerase (PARP) in staurosporine-treated cells. Staurosporine 174-187 poly(ADP-ribose) polymerase 1 Homo sapiens 165-169 10713065-4 2000 Overexpression of glycogen synthase kinase-3beta to levels 3.5 times that in control cells did not alter basal indices of apoptosis but potentiated staurosporine-induced activation of caspase-3, caspase-9, proteolysis of poly(ADP-ribose) polymerase, and morphological changes indicative of apoptosis. Staurosporine 148-161 poly(ADP-ribose) polymerase 1 Homo sapiens 221-248 10581530-6 1999 Cleavage of poly(ADP-ribose) polymerase (PARP) illustrated that the staurosporine treatment induced apoptosis in the cells within 6 h. Analysis of PARP cleavage indicated that treatment with DFMO accelerated the kinetics of progression of apoptosis but did not influence the sensitivity of cells to 10 nM-1 microM staurosporine. Staurosporine 68-81 poly(ADP-ribose) polymerase 1 Homo sapiens 12-39 10679755-3 2000 RESULTS: The LNCaP and TsuPr(1) lines exhibited quintessential apoptotic features in response to the pleiotropic apoptotic inducer staurosporine (STS): rapid cytochrome c translocation to the cytosol, proteolytic processing and catalytic activation of caspase-3 and -7, proteolytic inactivation of the death substrates DNA fragmentation factor (DFF) and poly-ADP-ribose polymerase (PARP), and TUNEL-positive polyfragmented nuclei. Staurosporine 131-144 poly(ADP-ribose) polymerase 1 Homo sapiens 354-380 10679755-3 2000 RESULTS: The LNCaP and TsuPr(1) lines exhibited quintessential apoptotic features in response to the pleiotropic apoptotic inducer staurosporine (STS): rapid cytochrome c translocation to the cytosol, proteolytic processing and catalytic activation of caspase-3 and -7, proteolytic inactivation of the death substrates DNA fragmentation factor (DFF) and poly-ADP-ribose polymerase (PARP), and TUNEL-positive polyfragmented nuclei. Staurosporine 131-144 poly(ADP-ribose) polymerase 1 Homo sapiens 382-386 10581530-6 1999 Cleavage of poly(ADP-ribose) polymerase (PARP) illustrated that the staurosporine treatment induced apoptosis in the cells within 6 h. Analysis of PARP cleavage indicated that treatment with DFMO accelerated the kinetics of progression of apoptosis but did not influence the sensitivity of cells to 10 nM-1 microM staurosporine. Staurosporine 68-81 poly(ADP-ribose) polymerase 1 Homo sapiens 41-45 10581530-6 1999 Cleavage of poly(ADP-ribose) polymerase (PARP) illustrated that the staurosporine treatment induced apoptosis in the cells within 6 h. Analysis of PARP cleavage indicated that treatment with DFMO accelerated the kinetics of progression of apoptosis but did not influence the sensitivity of cells to 10 nM-1 microM staurosporine. Staurosporine 68-81 poly(ADP-ribose) polymerase 1 Homo sapiens 147-151 10497198-10 1999 Furthermore, caspase-7 activation was concommitant with PARP cleavage in the caspase-3-deficient cell line MCF-7 in response to staurosporine treatment. Staurosporine 128-141 poly(ADP-ribose) polymerase 1 Homo sapiens 56-60 10037154-3 1999 The presence of EGF substantially reduced the in vitro Ac-DEVD-AMC hydrolytic activity and almost completely suppressed the intracellular cleavage of poly(ADP-ribose) polymerase in staurosporine- or wortmannin-treated cells. Staurosporine 181-194 poly(ADP-ribose) polymerase 1 Homo sapiens 150-177 16465208-6 1997 Here, we demonstrate that CPP32/Yama/Apopain, a member of the ICE/Ced-3 gene family, is processed during staurosporine-induced apoptosis in HeLa cells and that concomitant with CPP32 activation, two other proteins, poly (ADP-ribose) polymerase (PARP) and the U1-70 K small ribonucleoprotein, also undergo proteolysis. Staurosporine 105-118 poly(ADP-ribose) polymerase 1 Homo sapiens 215-243 16465208-6 1997 Here, we demonstrate that CPP32/Yama/Apopain, a member of the ICE/Ced-3 gene family, is processed during staurosporine-induced apoptosis in HeLa cells and that concomitant with CPP32 activation, two other proteins, poly (ADP-ribose) polymerase (PARP) and the U1-70 K small ribonucleoprotein, also undergo proteolysis. Staurosporine 105-118 poly(ADP-ribose) polymerase 1 Homo sapiens 245-249 10438458-7 1999 Expression of this mutant PARP increased the rate of staurosporine and tumor necrosis factor-alpha-induced apoptosis, at least in part by reducing the time interval required for the onset of caspase-3 activation and internucleosomal DNA fragmentation, as well as the generation of 50-kilobase pair DNA breaks, thought to be associated with early chromatin unfolding. Staurosporine 53-66 poly(ADP-ribose) polymerase 1 Homo sapiens 26-30 16465208-0 1997 Bcl-2 prevents activation of CPP32 cysteine protease and cleavage of poly (ADP-ribose) polymerase and U1-70 kD proteins in staurosporine-mediated apoptosis. Staurosporine 123-136 poly(ADP-ribose) polymerase 1 Homo sapiens 69-97