PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 9582501-7 1998 In fura-2 loaded BAG cells, BK (100 nM) induced a typical biphasic Ca2+ response composed of a rapid and transient increase of intracellular Ca2+ concentration [Ca2+]i which slowly declined to a level that remained above basal level for about 5 min. Fura-2 3-9 kininogen 1 Bos taurus 28-30 9767522-3 1998 METHODS: LPS- or BK-induced increments of intracellular Ca2+ were assessed by microspectrofluorimetry with fura-2 in passaged bovine aortic endothelial cells. Fura-2 107-113 kininogen 1 Bos taurus 17-19 7729011-4 1995 In cultured bovine aortic endothelial cells, extracellular application of bradykinin (BK, 10 to 20 mumol/L) caused transient (sometimes oscillatory) increase in [Ca2+]i, which was measured with the fluorescent Ca2+ indicator fura 2 and fluorescence imaging microscopy. Fura-2 225-231 kininogen 1 Bos taurus 74-84 8712453-4 1996 METHODS: The effect of halothane and isoflurane on the Ca2+ response to bradykinin of bovine aortic endothelial (BAE) cells was investigated using the fluorescent Ca2+ indicator fura-2. Fura-2 178-184 kininogen 1 Bos taurus 72-82 7729011-4 1995 In cultured bovine aortic endothelial cells, extracellular application of bradykinin (BK, 10 to 20 mumol/L) caused transient (sometimes oscillatory) increase in [Ca2+]i, which was measured with the fluorescent Ca2+ indicator fura 2 and fluorescence imaging microscopy. Fura-2 225-231 kininogen 1 Bos taurus 86-88 1996698-8 1991 These findings, together with our previously described results using fura-2, suggest that oxidant stress initially inhibits bradykinin-stimulated Ca2+ influx and later inhibits stimulated Ca2+ efflux. Fura-2 69-75 kininogen 1 Bos taurus 124-134 3263034-5 1988 In addition, bradykinin and melittin stimulated an increase in intracellular calcium concentration in fura-2 loaded endothelial cells and an increase in inositol 1,4,5-trisphosphate (Ins[1,4,5]P3) in cells prelabeled with 3H-myoinositol. Fura-2 102-108 kininogen 1 Bos taurus 13-23 2154452-1 1990 The effects of membrane potential on resting and bradykinin-stimulated changes in [Ca2+]i were measured in fura-2 loaded cultured endothelial cells from bovine atria by spectrofluorimetry. Fura-2 107-113 kininogen 1 Bos taurus 49-59 2909543-1 1989 Bradykinin-evoked rises in [Ca2+]i were measured in fura-2-loaded bovine pulmonary artery endothelial cell monolayers by dual wavelength excitation fluorimetry. Fura-2 52-58 kininogen 1 Bos taurus 0-10 2294097-1 1990 The effects of oxidant stress and inhibition of glutathione reductase on the bradykinin-stimulated changes in cytosolic free Ca2+ concentration ([Ca2+]i) of calf pulmonary artery endothelial cells were determined using the intracellular fluorescent probe, fura-2. Fura-2 256-262 kininogen 1 Bos taurus 77-87 2782436-4 1989 The effect of membrane depolarization with high K+ buffer on the bradykinin-stimulated changes in cytosolic Ca2+ was determined using the fluorescent Ca2+ indicator, fura-2. Fura-2 166-172 kininogen 1 Bos taurus 65-75 3263034-6 1988 Bradykinin stimulation produced transient increases in Ins(1,4,5)P3, fura-2 fluorescence and transient EDRF release. Fura-2 69-75 kininogen 1 Bos taurus 0-10 2444358-2 1987 After bradykinin (100 nM) exposure, endothelial cell associated fura-2 fluorescence peaked within 10-20 seconds and then declined to a steady level 2- to 3-fold above resting values. Fura-2 64-70 kininogen 1 Bos taurus 6-16 3136667-2 1988 In the present study, measurement of BK-induced changes in fura-2 fluorescence and 86Rb+ efflux were used to monitor changes in cytosolic Ca2+ and K+ permeability in cultured bovine aortic endothelial cells. Fura-2 59-65 kininogen 1 Bos taurus 37-39 2444358-4 1987 Chelation of extracellular calcium with EGTA before addition of bradykinin resulted in a substantial decrease in the fura-2 transient and elimination of the long-lasting component. Fura-2 117-123 kininogen 1 Bos taurus 64-74