PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 1440710-3 1992 METHODS: The fluorescent indicators fura-2 and bis-carboxyethyl-carboxyfluorescein were used to simultaneously measure [Ca2+]i and pHi with a fluorometer. Fura-2 36-42 glucose-6-phosphate isomerase Rattus norvegicus 131-134 2775282-5 1989 It is concluded that [Ca2+]i and pHi could be measured simultaneously in isolated myocyte using dual-loading of fura-2 and BCECF. Fura-2 112-118 glucose-6-phosphate isomerase Rattus norvegicus 33-36 1942669-1 1991 We describe a new system to measure changes in cytosolic free Ca concentration ([Ca2+]i) and pH (pHi) simultaneously using the dyes fura-2 and BCECF, and a random access camera with computer-operated synchronously-changing excitation and emission filters. Fura-2 132-138 glucose-6-phosphate isomerase Rattus norvegicus 97-100 2318379-0 1990 Effect of buffer systems and pHi on the measurement of [Ca2+]i with fura 2. Fura-2 68-74 glucose-6-phosphate isomerase Rattus norvegicus 29-32 2318379-7 1990 It was found that measurements of [Ca2+]i with fura 2 were influenced by shifts in pHi that occur when cells are incubated in either HEPES-buffered or CO2/HCO3- media of differing pHo values. Fura-2 47-53 glucose-6-phosphate isomerase Rattus norvegicus 83-86 2764121-4 1989 Changes in pHi and Cai2+ were measured with the fluorescent probes, 2,7-biscarboxyethyl-5(6)-carboxyfluorescein (BCECF) and fura-2. Fura-2 124-130 glucose-6-phosphate isomerase Rattus norvegicus 11-14 9679168-6 1998 The measurements of changes in pHi evoked by changes in pHo were employed in subsequent experiments to correct for the effects of changes in pHi on the Kd of fura-2 for Ca2+. Fura-2 158-164 glucose-6-phosphate isomerase Rattus norvegicus 141-144 9344760-3 1997 Compared with hypothyroid animals, myocytes isolated from hyperthyroid rat hearts demonstrated a significant: (1) increase in extent of shortening; (2) decrease in the time to peak contraction; (3) increase in the peak amplitude of the fura-2 fluorescence ratio; (4) decrease in pHi (DeltapHi=0. Fura-2 236-242 glucose-6-phosphate isomerase Rattus norvegicus 279-282 8368272-3 1993 Changes in pHi and Cai were measured with the fluorescent probes 2",7"-bis(carboxyethyl)-5,6-carboxyfluorescein and fura 2. Fura-2 116-122 glucose-6-phosphate isomerase Rattus norvegicus 11-14 1335472-9 1992 When the relationship between pHi and intracellular Ca2+ was determined with the fluorescent Ca2+ indicator, Fura-2, it was found that increasing pHo and treatment with ammonium chloride increased intracellular Ca2+, while sodium propionate and reducing pHi had no effect on intracellular Ca2+. Fura-2 109-115 glucose-6-phosphate isomerase Rattus norvegicus 30-33