PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 10535385-6 1999 Experiments performed on cytochalasin D-treated cells using Mn2+ as an indicator of Ca2+ influx quenched fura-2 fluorescence signals following vasopressin administration. Fura-2 105-111 arginine vasopressin Rattus norvegicus 143-154 9808762-2 1998 Immunoblot analysis revealed that A7r5 vascular smooth muscle cells expressed conventional (alpha), novel (delta and epsilon), and atypical (iota/lambda and mu) isoforms of protein kinase C. Stimulation of fura-2-loaded cells with 20 nM vasopressin induced a rapid transient increase in the intracellular concentration of calcium that was followed by a slowly declining component which was above baseline throughout the period of observation. Fura-2 206-212 arginine vasopressin Rattus norvegicus 237-248 9065736-2 1997 Using fura-2-loaded cells, we observed that the release induced by AVP occurred concurrently with calcium (Ca2+) mobilization from internal stores and entry of external Ca2+, whereas AlF4(-)-dependent arachidonate release was much slower and was not accompanied by intracellular Ca2+ mobilization. Fura-2 6-12 arginine vasopressin Rattus norvegicus 67-70 8780286-2 1996 Using digital imaging microscopy with the Ca2+ indicator fura-2, the effects of halothane on the intracellular [Ca2+] dynamics induced by arginine vasopressin (AVP) in the perinuclear region and cytosol in single cultured smooth muscle cells of rat aorta were studied. Fura-2 57-63 arginine vasopressin Rattus norvegicus 147-158 8914016-2 1996 The AVP-induced cellular signal transduction, including inositol 1,4,5-trisphosphate (IP3) production, fura-2 intracellular calcium measurements and cellular alkalinization, was significantly greater in cells of SHR than those of WKY. Fura-2 103-109 arginine vasopressin Rattus norvegicus 4-7 8913359-3 1996 By videomicroscopically examining Fura-2-loaded cells, we demonstrate that vasopressin induces a dose-dependent and receptor-mediated calcium influx fully inhibited by either 1 microM nifedipine or a pertussis toxin pretreatment and potentiated by 1 microM BAY K 8644. Fura-2 34-40 arginine vasopressin Rattus norvegicus 75-86 8780286-2 1996 Using digital imaging microscopy with the Ca2+ indicator fura-2, the effects of halothane on the intracellular [Ca2+] dynamics induced by arginine vasopressin (AVP) in the perinuclear region and cytosol in single cultured smooth muscle cells of rat aorta were studied. Fura-2 57-63 arginine vasopressin Rattus norvegicus 160-163 8756565-3 1996 Video microscopy experiments performed on fura-2-loaded adrenal medullary or adrenal glomerulosa cell primary cultures showed that vasopressin dose dependently mobilized intracellular calcium, suggesting that functional vasopressin receptors are expressed in these tissues. Fura-2 42-48 arginine vasopressin Rattus norvegicus 131-142 8756565-3 1996 Video microscopy experiments performed on fura-2-loaded adrenal medullary or adrenal glomerulosa cell primary cultures showed that vasopressin dose dependently mobilized intracellular calcium, suggesting that functional vasopressin receptors are expressed in these tissues. Fura-2 42-48 arginine vasopressin Rattus norvegicus 220-231 8387925-5 1993 Fluorescent ratio imaging with fura-2 revealed that addition of vasopressin to the cells results in an increase of [Ca2+]i which peaks within 20 s and does not return to resting levels during the 100 s observation period. Fura-2 31-37 arginine vasopressin Rattus norvegicus 64-75 8293556-7 1994 By use of Mn2+ quenching of intracellular fura 2 to measure divalent cation entry, the maximal rate of vasopressin-stimulated Mn2+ entry was impaired in simvastatin-treated cells by 52%. Fura-2 42-48 arginine vasopressin Rattus norvegicus 103-114 8611034-2 1996 In fura-2-loaded cells, AVP induced a rapid (0.5-2 min) transient increase in [Ca2+]i that was followed by a smaller sustained increase in [Ca2+]i. Fura-2 3-9 arginine vasopressin Rattus norvegicus 24-27 8589290-6 1995 Fura-2 fluorescence ratio was not significantly changed with angiotensin II, Ap3A, or Ap4A but increased with vasopressin, ATP, Ap5A, and Ap6A by 0.08 +/- 0.02 (N = 13), 0.04 +/- 0.02 (N = 13), 0.03 +/- 0.01 (N = 14), and 0.03 +/- 0.01 (N = 10), respectively. Fura-2 0-6 arginine vasopressin Rattus norvegicus 110-121 7666368-2 1995 Arg8-vasopressin (AVP)-regulated Ca2+ transport were investigated in fura-2-loaded A7r5 cells using both single cell and population measurements. Fura-2 69-75 arginine vasopressin Rattus norvegicus 0-16 7666368-2 1995 Arg8-vasopressin (AVP)-regulated Ca2+ transport were investigated in fura-2-loaded A7r5 cells using both single cell and population measurements. Fura-2 69-75 arginine vasopressin Rattus norvegicus 18-21 8068023-2 1994 Similarly, in cells microinjected with Fura-2 salt, vasopressin stimulated Mn2+ entry while 2,5-di-(t-butyl)hydroquinone or thapsigargin did not. Fura-2 39-45 arginine vasopressin Rattus norvegicus 52-63 8382636-2 1993 The flux through ROCCs was followed by quenching of fura-2 fluorescence due to the influx of extracellular Mn2+ induced by vasopressin. Fura-2 52-58 arginine vasopressin Rattus norvegicus 123-134 2170382-3 1990 In the presence of extracellular Mn2+, the Ca2(+)-mobilizing hormone vasopressin produced a severalfold stimulation of the basal rate of fura-2 fluorescence quenching as a result of Mn2+ influx; this effect was blocked by the presence of Ni2+ in the incubation medium. Fura-2 137-143 arginine vasopressin Rattus norvegicus 69-80 1393572-4 1992 Using Fura-2 loaded cells and dynamic ratio imaging, we have determined that arginine vasopressin (AVP) or V1- but not V2-receptor agonists, mobilise pituicyte intracellular Ca2+ ([Ca2+]i) in the absence of extracellular Ca2+. Fura-2 6-12 arginine vasopressin Rattus norvegicus 77-109 1329268-2 1992 Both AII and AVP specifically induce a transient increases in cytosolic free calcium independent of extracellular calcium or calcium channels activated by high potassium depolarization in VSM cells loaded with Fura-2. Fura-2 210-216 arginine vasopressin Rattus norvegicus 13-16 1939188-0 1991 Oscillations of intracellular calcium induced by vasopressin in individual fura-2-loaded mesangial cells. Fura-2 75-81 arginine vasopressin Rattus norvegicus 49-60 2548389-11 1989 The time course of the initial hyperpolarization was shown to be similar to the calcium transient observed in fura-2-loaded A10 cell suspensions after the application of AVP. Fura-2 110-116 arginine vasopressin Rattus norvegicus 170-173 17125880-6 2007 Also, the temporal pattern of vasopressin-elicited intracellular calcium oscillations studied on fura2-loaded cells was altered, with the duration of each Ca2+ peak being longer. Fura-2 97-102 arginine vasopressin Rattus norvegicus 30-41 2731227-2 1989 When stimulated with the agonists, angiotensin II and arginine vasopressin, cells incubated with low concentrations of fura-2 or indo-1 (less than 1 microM) produced Ca2+ transients characterized by a small increase followed by a dramatic decrease in fluorescence below the original baseline. Fura-2 119-125 arginine vasopressin Rattus norvegicus 63-74 3014901-6 1986 With fura-2 as the intracellular Ca2+ indicator, [Ca2+]f increased from 74 +/- 7 to a peak of 578 +/- 39 nM (n = 17) with 100 nM AVP. Fura-2 5-11 arginine vasopressin Rattus norvegicus 129-132