PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 17389736-11 2007 Fura-2 fluorescence-indicated that Ca(2+) entry in response to AngII stimulation was also dramatically inhibited by TRPC1 antibody and TRPC1-specific RNA interference. Fura-2 0-6 angiotensinogen Rattus norvegicus 63-68 24036592-7 2014 Functionally, the [Ca(2+)]i response to exogenous angiotensin II was enhanced in fura-2-loaded glomus cells dissociated from hypoxic rats when compared with those of the normoxic control animals. Fura-2 81-87 angiotensinogen Rattus norvegicus 50-64 17699555-9 2007 Fura-2 fluorescence measurements revealed that the ANG II-induced Ca(2+) influxes were markedly inhibited in MCs with TRPC6 knockdown, reminiscent of the impaired Ca(2+) entry in response to ANG II in high glucose-treated MCs. Fura-2 0-6 angiotensinogen Rattus norvegicus 51-57 15572518-5 2005 The effect of Src kinase inhibition on ANG II-induced intracellular free Ca(2+) concentration ([Ca(2+)](i)) responses was probed in fura 2-loaded preglomerular microvascular smooth muscle cells (PVSMCs) obtained from explants and studied after 3-5 days in culture. Fura-2 132-138 angiotensinogen Rattus norvegicus 39-45 15639476-4 2005 Ang II-induced calcium influx was studied in fura-2-loaded single vascular smooth muscle cells isolated from thoracic aorta of control and diabetic rats. Fura-2 45-51 angiotensinogen Rattus norvegicus 0-6 12388389-5 2003 Also, application of ANG II significantly increased intracellular Ca(2+) concentrations, which were measured with fura 2 dye. Fura-2 114-120 angiotensinogen Rattus norvegicus 21-27 12218532-5 2002 METHODS: The effect of 0.5-3% isoflurane was studied (1) on AngII (10 m)-induced intracellular Ca mobilization, intracellular Ca release from internal stores, and Ca influx in Fura-2 loaded cultured aortic vascular smooth muscle cell isolated from 6-week-old Wistar Kyoto and spontaneously hypertensive rats, using fluorescent imaging microscopy; and (2) on the organization of cytoskeletal elements, using immunofluorescence labeling. Fura-2 176-182 angiotensinogen Rattus norvegicus 60-65 12411465-8 2002 Moreover, angiotensin II stimulation of divalent cation influx (Mn2+ quench of fura-2 fluorescence) was decreased significantly by AG1478. Fura-2 79-85 angiotensinogen Rattus norvegicus 10-24 11009559-1 2000 Angiotensin II (Ang II)-induced Ca(2+) signaling was studied in isolated rat renal arterioles using fura-2. Fura-2 100-106 angiotensinogen Rattus norvegicus 0-22 10620186-10 2000 Confocal [Ca2+]i measurements using fura-2 or fluo-3 or fluo-4 on the single cell level show that some of the Ang II-mediated [Ca2+]i response originated from podocytes. Fura-2 36-42 angiotensinogen Rattus norvegicus 110-116 10413074-6 1999 In cultured rat aortic smooth muscle cells loaded with fura-2, increases in intracellular free-Ca2+ concentration ([Ca2+]i) induced by angiotensin II were markedly inhibited in cells incubated with simvastatin and atorvastatin but not pravastatin. Fura-2 55-61 angiotensinogen Rattus norvegicus 135-149 10513576-2 1999 Ni2+ (1 mM) significantly inhibited the transient increase in intracellular Ca2+ concentration ([Ca2+]i) induced by Ang II (100 nM) in aortic smooth muscle cells, as measured using fura-2. Fura-2 181-187 angiotensinogen Rattus norvegicus 116-122 12013483-12 1996 To determine whether the depression of vascular contraction by these agents was correlated with cell calcium changes, the angiotensin II-induced calcium mobilization was determined in Fura-2 loaded cells, before and after treatment with these inhibitors. Fura-2 184-190 angiotensinogen Rattus norvegicus 122-136 9689597-2 1998 To characterize signal transduction mechanisms of Ang II, we examined changes in intracellular Ca2+ concentration ([Ca2+]i) of fura-2-loaded cultured adult rat cardiac fibroblasts by fluorescence photometry. Fura-2 127-133 angiotensinogen Rattus norvegicus 50-56 8752105-2 1996 Bath perfusion or rapid puffer application of angiotensin II (Ang II) (1-100 nM) increased [Ca2+]i in both polygonal and stellate astroglia when measured using fura-2 imaging fluorescence microscopy. Fura-2 160-166 angiotensinogen Rattus norvegicus 46-60 8752105-2 1996 Bath perfusion or rapid puffer application of angiotensin II (Ang II) (1-100 nM) increased [Ca2+]i in both polygonal and stellate astroglia when measured using fura-2 imaging fluorescence microscopy. Fura-2 160-166 angiotensinogen Rattus norvegicus 62-68 10090417-4 1999 The mechanisms of angiotensin II-evoked calcium signaling were examined using fura-2-based fluorescent digital microscopy. Fura-2 78-84 angiotensinogen Rattus norvegicus 18-32 9444969-2 1998 In each strain the Ang II induced changes of [Ca2+]i were measured in cultured glomerular mesangial cells (MC) using the calcium-sensitive fluorescent dye, fura2. Fura-2 156-161 angiotensinogen Rattus norvegicus 19-25 8997399-1 1996 This study provides an initial characterization of basic morphological properties of cultures of vascular smooth muscle cells (VSMC) from rat preglomerular resistance vessels and of the functional coupling of angiotensin II (ANG II) receptors to cytosolic free calcium concentration ([Ca2+]i (fura 2 fluorescence photometry). Fura-2 293-299 angiotensinogen Rattus norvegicus 225-231 8761891-5 1996 Fura-2 was used to measure basal and angiotensin II-stimulated intracellular free calcium concentration. Fura-2 0-6 angiotensinogen Rattus norvegicus 37-51 8821822-4 1996 Fura-2 fluorescence was measured in an inverted microscope at 37 degrees C. Angiotensin II (0.1 nmol/liter) and ATP (0.1 mumol/liter) induced a rapid transient increase of [Ca2+]i, which was followed by a sustained plateau (N = 37 and N = 24). Fura-2 0-6 angiotensinogen Rattus norvegicus 76-90 7478924-9 1995 A half-maximal initial increase in the fura-2 fluorescence ratio was reached at 22 nmol/l, 0.9 micromol/l, 2.0 micromol/l and 4.0 micromol/l with Ang II, Ap3A, ATP and Ap4A, respectively. Fura-2 39-45 angiotensinogen Rattus norvegicus 146-152 1418854-7 1992 AII produced a concentration-related increase in calcium mobilization (fura-2 fluorescence) which was blocked by losartan (IC50 = 8.4 x 10(-8) mol/L) but not by PD123177 (10(-6) mol/L). Fura-2 71-77 angiotensinogen Rattus norvegicus 0-3 7479311-8 1995 In intact cells, angiotensin II caused a rapid increase in intracellular calcium (Ca2+) using Fura-2 as a Ca2+ indicator. Fura-2 94-100 angiotensinogen Rattus norvegicus 17-31 8329674-1 1993 The isolated rat renal arteriole technique was adapted for use in a fluorescence ratio imaging system in which angiotensin II (AII)-induced changes in lumen diameter and smooth muscle cell (SMC) cytosolic calcium [(Ca2+]i were serially determined for 4 min with Fura-2. Fura-2 262-268 angiotensinogen Rattus norvegicus 111-125 8329674-1 1993 The isolated rat renal arteriole technique was adapted for use in a fluorescence ratio imaging system in which angiotensin II (AII)-induced changes in lumen diameter and smooth muscle cell (SMC) cytosolic calcium [(Ca2+]i were serially determined for 4 min with Fura-2. Fura-2 262-268 angiotensinogen Rattus norvegicus 127-130 2260972-6 1990 The present study was undertaken to determine the effect of HgCl2 on ANG II-induced [Ca++]i increase using Fura-2. Fura-2 107-113 angiotensinogen Rattus norvegicus 69-75 1329268-2 1992 Both AII and AVP specifically induce a transient increases in cytosolic free calcium independent of extracellular calcium or calcium channels activated by high potassium depolarization in VSM cells loaded with Fura-2. Fura-2 210-216 angiotensinogen Rattus norvegicus 5-8 2731227-2 1989 When stimulated with the agonists, angiotensin II and arginine vasopressin, cells incubated with low concentrations of fura-2 or indo-1 (less than 1 microM) produced Ca2+ transients characterized by a small increase followed by a dramatic decrease in fluorescence below the original baseline. Fura-2 119-125 angiotensinogen Rattus norvegicus 35-49 1701016-9 1990 Ca2+ mobilization, measured by fura-2 fluorescence, was coincident with the increase in tyrosine phosphorylation in response to angiotensin II or thapsigargin. Fura-2 31-37 angiotensinogen Rattus norvegicus 128-142 2912686-2 1989 In 18 out of 44 individual rat granulosa cells loaded with fura-2, Ang II caused a rapid and transient increase in intracellular free calcium ion concentration, [Ca2+]i. Fura-2 59-65 angiotensinogen Rattus norvegicus 67-73 3399509-1 1988 The cytosolic calcium (Ca2+i) response to angiotensin II (Ang II) was examined in single rat zona glomerulosa cells by monitoring fura-2 fluorescence with microspectrofluorimetry. Fura-2 130-136 angiotensinogen Rattus norvegicus 42-56 3399509-1 1988 The cytosolic calcium (Ca2+i) response to angiotensin II (Ang II) was examined in single rat zona glomerulosa cells by monitoring fura-2 fluorescence with microspectrofluorimetry. Fura-2 130-136 angiotensinogen Rattus norvegicus 58-64