PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 6089174-3 1984 Comparison of the gene with that of the phenobarbital-inducible cytochrome P-450e showed that the gene structures for the two types of cytochrome P-450 differ greatly; the location, number, and size of intervening sequences are very dissimilar. Phenobarbital 40-53 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 64-81 2985574-3 1985 The insertion sites of the introns in this gene are well-conserved as compared with those of another MC-inducible cytochrome P-450c gene, but are completely different from those of a phenobarbital-inducible cytochrome P-450e gene. Phenobarbital 183-196 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 207-224 3972824-4 1985 Our data demonstrate that phenobarbital administration to rats resulted in marked increases in levels of hepatic mRNA for both cytochrome P-450b and cytochrome P-450e, with a 4- to 5-fold greater accumulation of P-450b mRNA vis a vis P-450e mRNA. Phenobarbital 26-39 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 149-166 3838174-2 1985 A cDNA clone for cytochrome P-450e, a phenobarbitone-inducible species in rat liver, has been isolated and characterized. Phenobarbital 38-52 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 17-34 3838174-4 1985 Inhibitors of heme synthesis such as cobalt chloride and 3-amino-1,2,4-triazole block the induction of cytochrome P-450e by phenobarbitone at the level of transcription. Phenobarbital 124-138 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 103-120 3488743-5 1986 Of the eleven MAbs, three also bound strongly to the phenobarbital-inducible rat liver cytochrome P-450 PB-4. Phenobarbital 53-66 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 87-108 3928374-5 1985 Analysis of clones from cDNA banks showed that two types of sequences are induced by phenobarbital corresponding to cytochrome P-450b and cytochrome P-450e respectively. Phenobarbital 85-98 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 138-155 2989270-19 1985 The simple repeated sequences of (CA)n which is present at -254 position in cytochrome P-450e gene is also observed at the equivalent position in cytochrome P-450b gene, but the repetitiveness is greatly reduced in cytochrome P-450b gene ((CA)5 for P-450b versus (CA)19 for P-450e), and this may somehow be related to the difference in the level of cytochrome P-450b and P-450e in the inductive phase of phenobarbital administration. Phenobarbital 404-417 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 76-93 6885818-7 1983 Although there are a small number of differences, DNA sequence analysis of the eight exons of the gene present in these genomic clones indicates that they encode residues 58 to 491 (the COOH terminus) of cytochrome P-450e, a major phenobarbital-induced isozyme. Phenobarbital 231-244 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 204-221 6885818-8 1983 It appears that the other cloned genes may contain only a small region of very strong homology to the cytochrome P-450e gene, a region which includes the exon encoding a tridecapeptide which is also present in two dissimilar forms of rabbit liver cytochrome P-450, one constitutive and one induced by phenobarbital. Phenobarbital 301-314 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 102-119 6885818-10 1983 One of these genes is likely to encode cytochrome P-450b, the major phenobarbital induced form of cytochrome P-450 whose mRNA is greater than 95% homologous to that encoding cytochrome P-450e. Phenobarbital 68-81 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 174-191 21988088-2 2011 As observed in liver, pretreatment of phenobarbital (PB) or phenytoin were found to increase the expression of CYP2B1, CYP2B2 and associated enzyme activity in PBL. Phenobarbital 38-51 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 119-125 21988088-2 2011 As observed in liver, pretreatment of phenobarbital (PB) or phenytoin were found to increase the expression of CYP2B1, CYP2B2 and associated enzyme activity in PBL. Phenobarbital 53-55 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 119-125 19737542-7 2010 The rat CYP2B2 gene and its closely related mouse homolog, Cyp2b10, are both strongly induced in liver by phenobarbital. Phenobarbital 106-119 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 8-14 21763762-7 2011 NDEA treatment following PB exposure increased CYP2B1 mRNA expression under all tested concentrations and also increased CYP2B2 expression at 21 and 105 mug/mL. Phenobarbital 25-27 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 121-127 19737542-8 2010 We found that there is phenobarbital-dependent recruitment of WRN to the promoter of the CYP2B2 gene as demonstrated by chromatin immunoprecipitation analysis. Phenobarbital 23-36 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 89-95 18486294-5 2008 Evaluation of the effects of co-treatment of rats with PB and DIM by a full factorial ANOVA showed that DIM decreased the PB-induced CYP2B1 and CYP2B2 mRNA expression levels, and the rates of 2- and 4-hydroxylation of E2, and total E2 metabolite formation. Phenobarbital 55-57 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 144-150 18670162-2 2008 In Experiment 1, oral administration of PB (0, 25, 50, 100 or 150 mg/kg/day) for 2 weeks induced increases in hepatic cytochrome P450 content and CYP2B expression, prolongation of coagulation time (activated partial thromboplastin time (APTT) and Thrombotest (TBT)) and an increase in anti-thrombin III (AT III) concentration in a dose-dependent manner. Phenobarbital 40-42 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 146-151 18670162-3 2008 In Experiment 2, PB administration (100 mg/kg/day) for up to 14 days produced time-dependent increases in hepatic cytochrome P450 content and CYP2B (CYP2B1 and CYP2B2) expression. Phenobarbital 17-19 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 142-147 18670162-3 2008 In Experiment 2, PB administration (100 mg/kg/day) for up to 14 days produced time-dependent increases in hepatic cytochrome P450 content and CYP2B (CYP2B1 and CYP2B2) expression. Phenobarbital 17-19 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 160-166 18486294-5 2008 Evaluation of the effects of co-treatment of rats with PB and DIM by a full factorial ANOVA showed that DIM decreased the PB-induced CYP2B1 and CYP2B2 mRNA expression levels, and the rates of 2- and 4-hydroxylation of E2, and total E2 metabolite formation. Phenobarbital 122-124 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 144-150 18528841-5 2008 PF2D was also found to be useful for the semiquantification of some representative cytochrome P450 family proteins (e.g., cytochrome P450 2B2) that were induced by PB treatment compared with untreated controls. Phenobarbital 164-166 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 122-141 18164277-0 2008 Cell shape, cell-cell contact, cell-extracellular matrix contact and cell polarity are all required for the maximum induction of CYP2B1 and CYP2B2 gene expression by phenobarbital in adult rat cultured hepatocytes. Phenobarbital 166-179 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 140-146 18164277-1 2008 The effect of cell shape, cell density, contact with extracellular matrix and cell polarity on the phenobarbital (PB)-induced gene expression of CYP2B1 and CYP2B2 (CYP2B1/2B2) in adult rat hepatocytes was investigated. Phenobarbital 99-112 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 156-162 18164277-1 2008 The effect of cell shape, cell density, contact with extracellular matrix and cell polarity on the phenobarbital (PB)-induced gene expression of CYP2B1 and CYP2B2 (CYP2B1/2B2) in adult rat hepatocytes was investigated. Phenobarbital 99-112 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 164-174 18164277-1 2008 The effect of cell shape, cell density, contact with extracellular matrix and cell polarity on the phenobarbital (PB)-induced gene expression of CYP2B1 and CYP2B2 (CYP2B1/2B2) in adult rat hepatocytes was investigated. Phenobarbital 114-116 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 156-162 18164277-1 2008 The effect of cell shape, cell density, contact with extracellular matrix and cell polarity on the phenobarbital (PB)-induced gene expression of CYP2B1 and CYP2B2 (CYP2B1/2B2) in adult rat hepatocytes was investigated. Phenobarbital 114-116 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 164-174 18164277-3 2008 Hepatocytes cultured on EHS gel showed a spherical cell shape and highly enhanced the induction of CYP2B1/2B2 gene expression by PB. Phenobarbital 129-131 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 99-109 18164277-8 2008 On the other hand, when hepatocytes were cultured on dishes coated with lower concentrations of laminin, they became round and greater induction of CYP2B1/2B2 gene expression by PB was observed. Phenobarbital 178-180 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 148-158 10529397-0 1999 Phosphorylation/Dephosphorylation steps are crucial for the induction of CYP2B1 and CYP2B2 gene expression by phenobarbital. Phenobarbital 110-123 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 84-90 15656786-0 2005 The CYP2B2 phenobarbital response unit contains binding sites for hepatocyte nuclear factor 4, PBX-PREP1, the thyroid hormone receptor beta and the liver X receptor. Phenobarbital 11-24 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 4-10 15656786-1 2005 A 163 bp enhancer in the CYP2B2 5" flank confers PB (phenobarbital) inducibility and constitutes a PBRU (PB response unit). Phenobarbital 49-51 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 25-31 15656786-1 2005 A 163 bp enhancer in the CYP2B2 5" flank confers PB (phenobarbital) inducibility and constitutes a PBRU (PB response unit). Phenobarbital 53-66 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 25-31 12920159-4 2003 CYP2B2 expression was limited to the livers of PB-treated male and female rats and was not detected in spleen. Phenobarbital 47-49 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 0-6 11377393-0 2001 Functional analysis of the phenobarbital-responsive unit in rat CYP2B2. Phenobarbital 27-40 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 64-70 11377393-1 2001 An 163-bp fragment of the rat cytochrome P450 gene, CYP2B2 has been shown to contain sequences that mediate phenobarbital (PB) responsiveness of this gene. Phenobarbital 108-121 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 52-58 11377393-1 2001 An 163-bp fragment of the rat cytochrome P450 gene, CYP2B2 has been shown to contain sequences that mediate phenobarbital (PB) responsiveness of this gene. Phenobarbital 123-125 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 52-58 11082433-0 2000 Sequence analyses of CYP2B genes and catalytic profiles for P450s in Qdj:Sprague-dawley rats that lack response to the phenobarbital-mediated induction of CYP2B2. Phenobarbital 119-132 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 155-161 11082433-1 2000 The Qdj:Sprague-Dawley (SD) rat is a mutant strain lacking in phenobarbital (PB)-mediated induction of CYP2B2. Phenobarbital 62-75 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 103-109 11082433-1 2000 The Qdj:Sprague-Dawley (SD) rat is a mutant strain lacking in phenobarbital (PB)-mediated induction of CYP2B2. Phenobarbital 77-79 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 103-109 10945841-5 2000 with four daily doses of PB demonstrated markedly induced levels of CYP2B1, CYP2B2, and CYP3A1 mRNA in the striatum and cerebellum. Phenobarbital 25-27 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 76-82 10945841-6 2000 In contrast, 1 or 2 days of PB treatment resulted in unchanged or even slightly decreased levels of CYP2B1 and CYP2B2 in the brain, although the latter treatments produced marked induction of the corresponding genes in the liver. Phenobarbital 28-30 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 111-117 10945841-8 2000 Substantial activation of cerebral CYP2B1, CYP2B2, and CYP3A1 mRNA levels also resulted when animals were treated with the neuroactive drugs diphenylhydantoin and amitryptiline, and with the potential PB-like xenobiotic inducers trans-stilbene oxide and diallyl sulfide, whereas dichlorodiphenyltrichloroethane was less efficacious. Phenobarbital 201-203 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 43-49 10788445-4 2000 This motif resembles phenobarbital response elements in the flanking regions of three phenobarbital-inducible genes, rat CYP2B2, mouse Cyp2b10, and human CYP2B6. Phenobarbital 21-34 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 121-127 10788445-4 2000 This motif resembles phenobarbital response elements in the flanking regions of three phenobarbital-inducible genes, rat CYP2B2, mouse Cyp2b10, and human CYP2B6. Phenobarbital 86-99 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 121-127 16421897-8 2005 Notably, phenobarbital resulted in significant induction of CYP2B1, CYP2B2, CYP2C6, CYP2C13, CYP2E1, CYP3A1, and CYP3A2. Phenobarbital 9-22 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 68-74 15345328-1 2004 A sequence critical for phenobarbital (PB) induction, the PB response unit (PBRU), situated upstream of the rat CYP2B1 and CYP2B2 genes, includes two nuclear receptor binding sites, NR1 and NR2. Phenobarbital 24-37 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 123-129 15345328-1 2004 A sequence critical for phenobarbital (PB) induction, the PB response unit (PBRU), situated upstream of the rat CYP2B1 and CYP2B2 genes, includes two nuclear receptor binding sites, NR1 and NR2. Phenobarbital 39-41 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 123-129 12028663-7 2002 The major phenobarbital (PB)-inducible forms of P450, CYP2B1, CYP2B2, CYP3A2 and CYP2C6, were substantially induced by 3-MeSO(2)-TetraBrB, but CYP1A1 and CYP1A2 were not. Phenobarbital 10-23 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 62-68 12028663-7 2002 The major phenobarbital (PB)-inducible forms of P450, CYP2B1, CYP2B2, CYP3A2 and CYP2C6, were substantially induced by 3-MeSO(2)-TetraBrB, but CYP1A1 and CYP1A2 were not. Phenobarbital 25-27 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 62-68 11518807-4 2001 Thus, the phenobarbital-inducible enhancer units of the mouse Cyp2b10, rat CYP2B2, and human CYP2B6 genes were activated in reporter gene assays by the same compounds that activate the chicken CYP2H1 phenobarbital-inducible enhancer units. Phenobarbital 10-23 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 75-81 11518807-4 2001 Thus, the phenobarbital-inducible enhancer units of the mouse Cyp2b10, rat CYP2B2, and human CYP2B6 genes were activated in reporter gene assays by the same compounds that activate the chicken CYP2H1 phenobarbital-inducible enhancer units. Phenobarbital 200-213 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 75-81 10529397-1 1999 The effects of several protein kinase activators and protein phosphatase inhibitors on the phenobarbital (PB)-induced gene expression of CYP2B1 and CYP2B2 (CYP2B1/2B2) in adult rat hepatocytes were investigated. Phenobarbital 91-104 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 148-154 10529397-1 1999 The effects of several protein kinase activators and protein phosphatase inhibitors on the phenobarbital (PB)-induced gene expression of CYP2B1 and CYP2B2 (CYP2B1/2B2) in adult rat hepatocytes were investigated. Phenobarbital 91-104 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 156-166 10529397-1 1999 The effects of several protein kinase activators and protein phosphatase inhibitors on the phenobarbital (PB)-induced gene expression of CYP2B1 and CYP2B2 (CYP2B1/2B2) in adult rat hepatocytes were investigated. Phenobarbital 106-108 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 148-154 10529397-1 1999 The effects of several protein kinase activators and protein phosphatase inhibitors on the phenobarbital (PB)-induced gene expression of CYP2B1 and CYP2B2 (CYP2B1/2B2) in adult rat hepatocytes were investigated. Phenobarbital 106-108 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 156-166 10796078-0 1999 Positive regulation of the rat CYP2B2 phenobarbital response unit by the nuclear receptor hexamer half-site.nuclear factor 1 complex. Phenobarbital 38-51 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 31-37 10423399-4 1999 The constitutive expression of CYP2B1 and CYP2B2 in liver is low, but inducible by PB, whereas the pulmonary expression of CYP2B1 is not induced by PB. Phenobarbital 83-85 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 42-48 10796078-1 1999 A distal 163-bp fragment mediates phenobarbital responsiveness of the rat CYP2B2 gene. Phenobarbital 34-47 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 74-80 10796078-6 1999 We introduced into the rat CYP2B2 hexamer half-site the specific mutational change previously introduced into the Cyp2b10 sequence, where its effect was to increase the basal level of expression and to abolish phenobarbital responsiveness. Phenobarbital 210-223 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 27-33 10036216-1 1999 Phenobarbital (PB) and many structurally unrelated chemicals induce the protein and mRNA of P450 cytochromes CYP2B1, CYP2B2, CYP3A1, and specific phase II enzymes to a greater extent in Fischer 344 (F344) than in Wistar Furth (WF) female rats. Phenobarbital 0-13 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 117-123 10072770-0 1999 Phenobarbital responsiveness conferred by the 5"-flanking region of the rat CYP2B2 gene in transgenic mice. Phenobarbital 0-13 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 76-82 10036216-1 1999 Phenobarbital (PB) and many structurally unrelated chemicals induce the protein and mRNA of P450 cytochromes CYP2B1, CYP2B2, CYP3A1, and specific phase II enzymes to a greater extent in Fischer 344 (F344) than in Wistar Furth (WF) female rats. Phenobarbital 15-17 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 117-123 9628589-2 1998 A 163-bp fragment at about -2.2 Kb in CYP2B2 has been shown to mediate phenobarbital induction in primary rat hepatocytes (Trottier, et al. Phenobarbital 71-84 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 38-44 9678251-0 1998 Identification of sequences involved in both basal expression and phenobarbital induction of a CYP2B2 gene using in vitro transcription system. Phenobarbital 66-79 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 95-101 9678251-7 1998 The use of synthetic oligonucleotides as competitor in vitro transcription assays indicated the importance for transcriptional control of the CYP2B2 gene of sequences located between -183 and -199 and -31 and -72 that were identified previously [1] as binding rat liver nuclear proteins that are enriched or activated in vivo by phenobarbital. Phenobarbital 329-342 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 142-148 9628589-14 1998 These studies indicate that the CYP2B2 phenobarbital-responsive enhancer contains multiple constitutive and phenobarbital-responsive elements. Phenobarbital 39-52 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 32-38 9628589-14 1998 These studies indicate that the CYP2B2 phenobarbital-responsive enhancer contains multiple constitutive and phenobarbital-responsive elements. Phenobarbital 108-121 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 32-38 9890442-3 1999 Culturing in the absence of insulin produced 1.5-2-fold increases in the induction magnitude of CYP2B1 and CYP2B2 mRNA expression resulting from PB exposures, without altering the bell-shaped dose-response curve characteristic of this agent. Phenobarbital 145-147 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 107-113 9525968-0 1998 The CYP2B2 phenobarbital response unit contains an accessory factor element and a putative glucocorticoid response element essential for conferring maximal phenobarbital responsiveness. Phenobarbital 11-24 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 4-10 9630454-1 1998 Phenobarbital (PB)-mediated induction of five forms of cytochrome P450 (CYP2B1, CYP2B2, CYP3A1, CYP2A1, and CYP2C6) and epoxide hydrolase is highly suppressed, at the transcriptional level, in Wistar Furth (WF) relative to Fischer 344 (F344) female rats. Phenobarbital 0-13 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 80-86 9630454-1 1998 Phenobarbital (PB)-mediated induction of five forms of cytochrome P450 (CYP2B1, CYP2B2, CYP3A1, CYP2A1, and CYP2C6) and epoxide hydrolase is highly suppressed, at the transcriptional level, in Wistar Furth (WF) relative to Fischer 344 (F344) female rats. Phenobarbital 15-17 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 80-86 9525968-0 1998 The CYP2B2 phenobarbital response unit contains an accessory factor element and a putative glucocorticoid response element essential for conferring maximal phenobarbital responsiveness. Phenobarbital 156-169 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 4-10 9525968-2 1998 One of the major unsolved problems in xenobiotic metabolism is the molecular mechanism whereby phenobarbital induces hepatic enzymes, particularly CYP2B1 and CYP2B2 in rat liver. Phenobarbital 95-108 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 158-164 9525968-3 1998 By using primary rat hepatocytes for transfection analyses, we previously identified in the CYP2B2 5"-flank a 163-base pair Sau3AI fragment that confers phenobarbital inducibility on a cat reporter gene and that has the properties of a transcriptional enhancer. Phenobarbital 153-166 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 92-98 9525968-9 1998 Taken together, the results indicate that phenobarbital induction of CYP2B2 requires interactions among multiple regulatory proteins and cis-acting elements constituting a phenobarbital response unit. Phenobarbital 42-55 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 69-75 9525968-9 1998 Taken together, the results indicate that phenobarbital induction of CYP2B2 requires interactions among multiple regulatory proteins and cis-acting elements constituting a phenobarbital response unit. Phenobarbital 172-185 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 69-75 9262383-4 1997 PB induction responses were assessed by use of specific hybridization probes to CYP2B1 and CYP2B2 mRNAs. Phenobarbital 0-2 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 91-97 9664231-4 1998 At equivalent 100 microM concentrations, the C6 and C8 MDB congeners were more effective than the prototypical inducer phenobarbital (PB) with respect to induction potency of CYP2B1, CYP2B2, and CYP3A1 gene expression. Phenobarbital 134-136 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 183-189 9280408-0 1997 Effect of ovariectomy and androgen on phenobarbital induction of hepatic CYP2B1 and CYP2B2 in Sprague-Dawley rats. Phenobarbital 38-51 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 84-90 9280408-1 1997 The purpose of this study was to investigate the impact of prepubertal ovariectomy and postpubertal administration of testosterone on inducibility of rat hepatic CYP2B1 and CYP2B2 by phenobarbital. Phenobarbital 183-196 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 173-179 8912644-0 1996 In vivo phenobarbital treatment increases protein binding to a putative AP-1 site in the CYP2B2 promoter. Phenobarbital 8-21 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 89-95 9063451-9 1997 A 2-3-fold increase over the basal level of chloramphenicol acetyltransferase activity was observed in phenobarbital-treated co-cultures transfected with the phenobarbital-responsive element construct, although phenobarbital had no effect on large CYP2B1 or CYP2B2 promoter fragments. Phenobarbital 103-116 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 258-264 9063451-9 1997 A 2-3-fold increase over the basal level of chloramphenicol acetyltransferase activity was observed in phenobarbital-treated co-cultures transfected with the phenobarbital-responsive element construct, although phenobarbital had no effect on large CYP2B1 or CYP2B2 promoter fragments. Phenobarbital 158-171 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 258-264 9063451-9 1997 A 2-3-fold increase over the basal level of chloramphenicol acetyltransferase activity was observed in phenobarbital-treated co-cultures transfected with the phenobarbital-responsive element construct, although phenobarbital had no effect on large CYP2B1 or CYP2B2 promoter fragments. Phenobarbital 158-171 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 258-264 8990265-6 1997 Phenobarbital-inducible CYP2B1 and CYP2B2 were constitutively expressed in dwarf rats, although substantially absent in normal SD rats. Phenobarbital 0-13 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 35-41 9101035-12 1997 The increase of bromide formation after CDBM administration in phenobarbital (PB)-pretreated rats indicated that cytochrome P-450 2B1 and 2B2 (CYP2B1 and CYP2B2) play a role as catalysts of the CDBM biotransformation. Phenobarbital 63-76 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 154-160 9101035-12 1997 The increase of bromide formation after CDBM administration in phenobarbital (PB)-pretreated rats indicated that cytochrome P-450 2B1 and 2B2 (CYP2B1 and CYP2B2) play a role as catalysts of the CDBM biotransformation. Phenobarbital 78-80 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 154-160 8954103-5 1996 Since the PB-induced UDP-glucuronosyltransferase gene expression was not reduced by insulin, the suppressive effect of insulin was considered to be specific to the CYP2B1/2B2 gene. Phenobarbital 10-12 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 164-174 8954103-6 1996 These results demonstrate that insulin in media masks the PB-induced expression of the CYP2B1/2B2 gene in conventional monolayer hepatocytes and that the use of insulin-free media with primary hepatocytes provides a useful tool for investigating the molecular mechanism of CYP2B1/2B2 gene expression. Phenobarbital 58-60 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 87-97 8954103-6 1996 These results demonstrate that insulin in media masks the PB-induced expression of the CYP2B1/2B2 gene in conventional monolayer hepatocytes and that the use of insulin-free media with primary hepatocytes provides a useful tool for investigating the molecular mechanism of CYP2B1/2B2 gene expression. Phenobarbital 58-60 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 273-283 8954103-0 1996 Insulin suppresses the induction of CYP2B1 and CYP2B2 gene expression by phenobarbital in adult rat cultured hepatocytes. Phenobarbital 73-86 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 47-53 8954103-1 1996 The effect of insulin on the phenobarbital (PB)-induced gene expression of CYP2B1 and CYP2B2 (CYP2B1/2B2) in adult rat hepatocytes was investigated. Phenobarbital 29-42 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 86-92 8954103-1 1996 The effect of insulin on the phenobarbital (PB)-induced gene expression of CYP2B1 and CYP2B2 (CYP2B1/2B2) in adult rat hepatocytes was investigated. Phenobarbital 29-42 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 94-104 8954103-1 1996 The effect of insulin on the phenobarbital (PB)-induced gene expression of CYP2B1 and CYP2B2 (CYP2B1/2B2) in adult rat hepatocytes was investigated. Phenobarbital 44-46 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 86-92 8954103-1 1996 The effect of insulin on the phenobarbital (PB)-induced gene expression of CYP2B1 and CYP2B2 (CYP2B1/2B2) in adult rat hepatocytes was investigated. Phenobarbital 44-46 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 94-104 8954103-3 1996 Although the induction by PB was not seen in monolayer hepatocytes cultured on type I collagen under standard culture conditions, the induced expression of the CYP2B1/2B2 gene was observed in monolayer hepatocytes by removing insulin from the medium. Phenobarbital 26-28 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 160-170 8954103-4 1996 Further, we succeeded in maintaining the prolonged induction of CYP2B1/2B2 by PB in monolayer hepatocytes by using a medium containing dexamethasone but not insulin. Phenobarbital 78-80 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 64-74 8912644-5 1996 Likewise, a putative AP-1 site, identified at -1441 in the CYP2B2 5"-flanking region, also formed a sequence specific DNA/protein complex which was enhanced after PB exposure. Phenobarbital 163-165 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 59-65 8643092-0 1996 Phenobarbital induction of hepatic CYP2B1 and CYP2B2: pretranscriptional and post-transcriptional effects of gender, adult age, and phenobarbital dose. Phenobarbital 0-13 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 46-52 8937440-0 1996 New proteins in the rat CYP2B subfamily: presence in liver microsomes of the constitutive CYP2B3 protein and the phenobarbital-inducible protein product of alternatively spliced CYP2B2 mRNA. Phenobarbital 113-126 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 178-184 8798596-0 1996 Phenobarbital induction mediated by a distal CYP2B2 sequence in rat liver transiently transfected in situ. Phenobarbital 0-13 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 45-51 8798596-12 1996 In this system, a distal CYP2B2 element mediates a response to phenobarbital, and proximal elements, including the Barbie box, are not required for the induction. Phenobarbital 63-76 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 25-31 8769571-1 1996 Cytochromes P450 2B1 and 2B2 (CYP2B1 and CYP2B2) are well-known phenobarbital-inducible genes in rat liver. Phenobarbital 64-77 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 41-47 8643092-2 1996 In this regard, we examined the interactions of gender, adult age, and barbiturate dose on the course of phenobarbital induction of hepatic CYP2B1 and CYP2B2. Phenobarbital 105-118 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 151-157 8643092-3 1996 We observed that femaleness and youth were associated with the greatest inhibition, so that both the rate and initiation of CYP2B1 and CYP2B2 induction were suppressed most in the young adult (65 days of age) females, followed by the mature adult (150 days of age) females and then by the young adult males, with the mature adult males exhibiting the least suppression of phenobarbital induction. Phenobarbital 372-385 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 135-141 8643092-9 1996 The considerably elevated growth hormone pulse amplitudes observed in the young rats of both genders seem to be an additional inhibitory signal antagonizing phenobarbital induction of CYP2B1 and CYP2B2. Phenobarbital 157-170 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 195-201 8643092-13 1996 Unlike use of the 10 mg/kg dose, CYP2B1 and CYP2B2 induction by phenobarbital at 1 mg/kg was unaffected by age or gender. Phenobarbital 64-77 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 44-50 8408026-0 1993 Phenobarbital induction and tissue-specific expression of the rat CYP2B2 gene in transgenic mice. Phenobarbital 0-13 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 66-72 8573202-1 1996 Cytochrome P450 (CYP) 2B1 and 2B2 are encoded by two closely related genes, CYP2B1 and CYP2B2, that are expressed at low levels in adult rat liver but are induced markedly by the administration of the drug phenobarbital (PB) or other structurally unrelated hydrophobic compounds to animals. Phenobarbital 206-219 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 87-93 8615872-10 1995 In parallel experiments, western blot analysis of immunoreactive CYP2B1 and CYP2B2 protein showed that PB, the PCB mixtures, and congeners induced both proteins. Phenobarbital 103-105 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 76-82 7646073-0 1995 Phenobarbital induction of CYP2B1, CYP2B2, and CYP3A1 in rat liver: genetic differences in a common regulatory mechanism. Phenobarbital 0-13 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 35-41 7646073-3 1995 Immunoblot analysis revealed that the strain-specific differences of phenobarbital responsiveness (10-fold for CYP2B1, CYP2B2, and CYP3A1 in females) are much smaller in male animals and are also greatly diminished by hypophysectomy. Phenobarbital 69-82 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 119-125 7646073-4 1995 Partial depletion of thyroid hormone and growth hormone levels by methimazole treatment was equally as effective as hypophysectomy in elevating phenobarbital-induced levels of CYP2B1, CYP2B2, and CYP3A1 in Wistar Furth rats, while the Fischer strain was unaffected. Phenobarbital 144-157 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 184-190 7773300-4 1995 In the present report, we have characterized culture conditions further by examining individual and interactive effects of dexamethasone (Dex) and PB on CYP2B1, CYP2B2, and CYP3A1 expression. Phenobarbital 147-149 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 161-167 7773300-7 1995 In contrast, at levels > 10(-7) M, Dex profoundly inhibited PB induction of the CYP2B1 and CYP2B2 genes. Phenobarbital 63-65 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 94-100 7773300-9 1995 Similarly, concentrations of PB > 0.5 mM resulted in substantially reduced levels of CYP2B1 and CYP2B2 induction than those attainable at lower PB concentrations. Phenobarbital 29-31 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 99-105 7773300-10 1995 These results suggest that Dex and PB function cooperatively to regulate the CYP2B1 and CYP2B2 genes, and that composite interactions may either negatively or positively regulate expression, in a concentration-dependent manner. Phenobarbital 35-37 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 88-94 7979401-1 1994 The phenobarbital (PB)-mediated expression of five forms of cytochrome P450 (CYP2A1, CYP2B1, CYP2B2, CYP2C6, and CYP3A1) and epoxide hydrolase has been examined in male and female rats from three inbred strains [Fischer (F344), Wistar Furth (WF), and Wistar Kyoto (WK)]. Phenobarbital 4-17 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 93-99 7979401-1 1994 The phenobarbital (PB)-mediated expression of five forms of cytochrome P450 (CYP2A1, CYP2B1, CYP2B2, CYP2C6, and CYP3A1) and epoxide hydrolase has been examined in male and female rats from three inbred strains [Fischer (F344), Wistar Furth (WF), and Wistar Kyoto (WK)]. Phenobarbital 19-21 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 93-99 8068204-0 1994 Interaction of proteins with a cytochrome P450 2B2 gene promoter: identification of two DNA sequences that bind proteins that are enriched or activated in response to phenobarbital. Phenobarbital 167-180 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 31-50 8068204-8 1994 These two DNA sequences and their cognate binding proteins may play a role in the induction of CYP2B2 gene expression in response to phenobarbital. Phenobarbital 133-146 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 95-101 8018775-10 1994 After treatment of rats with PB both CYP2B1 and CYP2B2 were observed in the liver, while only the former was found in the lung and kidney. Phenobarbital 29-31 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 48-54 8138968-1 1994 The present study describes the effects of 2,2",4,4",5,5"-hexachlorobiphenyl, a "phenobarbital-like" inducer of hepatic cytochrome P450, on the CYP2B1 and CYP2B2 enzymes in the phenotypically obese fa/fa Zucker rat. Phenobarbital 81-94 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 155-161 8408026-5 1993 These results demonstrate that, in vivo, phenobarbital induction and tissue-specific control requires interaction of regulatory elements far upstream of the core CYP2B2 promoter region and upstream of motifs indicated previously as determinants of phenobarbital responsiveness. Phenobarbital 41-54 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 162-168 8408026-5 1993 These results demonstrate that, in vivo, phenobarbital induction and tissue-specific control requires interaction of regulatory elements far upstream of the core CYP2B2 promoter region and upstream of motifs indicated previously as determinants of phenobarbital responsiveness. Phenobarbital 248-261 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 162-168 8573202-1 1996 Cytochrome P450 (CYP) 2B1 and 2B2 are encoded by two closely related genes, CYP2B1 and CYP2B2, that are expressed at low levels in adult rat liver but are induced markedly by the administration of the drug phenobarbital (PB) or other structurally unrelated hydrophobic compounds to animals. Phenobarbital 221-223 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 87-93 8577819-7 1995 Based on relative levels of immunoreactive protein, the phenobarbital-inducible isoforms, CYP2B1 and CYP2B2, are most susceptible to T3-mediated suppression. Phenobarbital 56-69 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 101-107 7607552-1 1995 Cytochrome P450 2B1, encoded by CYP2B1, and cytochrome P450 2B2, encoded by CYP2B2, are inducible in rat liver by phenobarbital (PB). Phenobarbital 114-127 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 44-63 7607552-1 1995 Cytochrome P450 2B1, encoded by CYP2B1, and cytochrome P450 2B2, encoded by CYP2B2, are inducible in rat liver by phenobarbital (PB). Phenobarbital 114-127 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 76-82 7607552-1 1995 Cytochrome P450 2B1, encoded by CYP2B1, and cytochrome P450 2B2, encoded by CYP2B2, are inducible in rat liver by phenobarbital (PB). Phenobarbital 129-131 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 44-63 7607552-1 1995 Cytochrome P450 2B1, encoded by CYP2B1, and cytochrome P450 2B2, encoded by CYP2B2, are inducible in rat liver by phenobarbital (PB). Phenobarbital 129-131 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 76-82 7759530-4 1995 PB-inducible responses were measured in hepatocytes by hybridization to cytochrome P450 (CYP) CYP2B1, CYP2B2, and CYP3A1 mRNAs. Phenobarbital 0-2 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 102-108 1445240-1 1992 The major phenobarbital-inducible rat hepatic cytochromes P-450, CYP2B1 and CYP2B2, are the paradigmatic members of a cytochrome P-450 gene subfamily that contains at least seven additional members. Phenobarbital 10-23 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 76-82 8232228-6 1993 Transfection of promoter constructs containing a reporter gene whose expression is driven by a 1.4-kilobase 5" flanking segment of the CYP2B1 or CYP2B2 genes, which are highly inducible by phenobarbital in rat liver, led to > 3-fold increases in reporter gene activity in the presence of the drug. Phenobarbital 189-202 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 145-151 8232228-8 1993 The RU486 inhibition of the phenobarbital induction of both the endogenous CPY2C6 gene and the transfected CYP2B1 and CYP2B2 promoter constructs leads us to propose a model whereby the drug acts indirectly to cause the accumulation of an endogenous steroid, and this molecule, acting via its receptor, would be the direct inducer of cytochromes P450. Phenobarbital 28-41 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 118-124 8512318-1 1993 The present study describes the mechanism of the dampened induction of the CYP2B1 and CYP2B2 genes following phenobarbital treatment in the phenotypically obese fa/fa Zucker rat. Phenobarbital 109-122 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 86-92 2233688-0 1990 Developmental expression and in situ localization of the phenobarbital-inducible rat hepatic mRNAs for cytochromes CYP2B1, CYP2B2, CYP2C6, and CYP3A1. Phenobarbital 57-70 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 123-129 1513322-1 1992 The absence of phenobarbital (PB)-inducible cytochrome P450 2B2 (CYP2B2) in hepatic microsomes from Marshall 520 (M520) and Wistar Munich (WM) inbred strains of rat was previously reported [Biochem. Phenobarbital 30-32 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 65-71 1513322-4 1992 In the present study, solution hybridization was used to quantify PB-induced CYP2B2 and CYP2B1 mRNAs in livers from M520, WM, and outbred Sprague-Dawley rats, as well as additional inbred strains that express all known electrophoretic phenotypes for both of these closely related isozymes. Phenobarbital 66-68 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 77-83 2233688-6 1990 An exception to this trend was observed for rats of gestational day 22, which exhibited transiently increased constitutive levels of CYP2B1, CYP2B2, and CYP3A1 mRNAs, such that PB-induced levels were not elevated over those observed in untreated animals. Phenobarbital 177-179 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 141-147 2233688-9 1990 These results were in contrast to data obtained previously for PB-inducible CYP2B1 and CYP2B2 mRNAs, which were distributed homogeneously across the hepatic lobule except for cells in the immediate vicinity of the periportal tract. Phenobarbital 63-65 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 87-93 2323573-1 1990 Cytochrome P450e (P450IIB2) is a phenobarbital(PB)-inducible member of the rat liver P450IIB subfamily. Phenobarbital 33-46 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 0-16 2377462-1 1990 The rat cytochrome P450 CYP2B2 gene encodes one of the two major phenobarbital-inducible forms of hepatic microsomal cytochrome P-450. Phenobarbital 65-78 cytochrome P450, family 2, subfamily b, polypeptide 2 Rattus norvegicus 24-30