PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 25409894-0 2015 Characterization of CYP2B6 in a CYP2B6-humanized mouse model: inducibility in the liver by phenobarbital and dexamethasone and role in nicotine metabolism in vivo. Phenobarbital 91-104 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 20-26 25409894-2 2015 The inducibility of CYP2B6 by phenobarbital (PB) and dexamethasone (DEX), known inducers of CYP2B6 in human liver, was examined in the TG mice, as well as in TG/Cyp2abfgs-null (or "CYP2B6-humanized") mice. Phenobarbital 30-43 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 20-26 25409894-2 2015 The inducibility of CYP2B6 by phenobarbital (PB) and dexamethasone (DEX), known inducers of CYP2B6 in human liver, was examined in the TG mice, as well as in TG/Cyp2abfgs-null (or "CYP2B6-humanized") mice. Phenobarbital 45-47 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 20-26 25409894-2 2015 The inducibility of CYP2B6 by phenobarbital (PB) and dexamethasone (DEX), known inducers of CYP2B6 in human liver, was examined in the TG mice, as well as in TG/Cyp2abfgs-null (or "CYP2B6-humanized") mice. Phenobarbital 45-47 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 92-98 25409894-2 2015 The inducibility of CYP2B6 by phenobarbital (PB) and dexamethasone (DEX), known inducers of CYP2B6 in human liver, was examined in the TG mice, as well as in TG/Cyp2abfgs-null (or "CYP2B6-humanized") mice. Phenobarbital 45-47 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 92-98 25409894-3 2015 Hepatic expression of CYP2B6 mRNA and protein was greatly induced by PB or DEX treatment in both TG and TG/Cyp2abfgs-null mice. Phenobarbital 69-71 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 22-28 25409894-5 2015 In PB-treated mice, the rates of hepatic microsomal hydroxybupropion formation (at 50 muM bupropion) were >4-fold higher in TG/Cyp2abfgs-null than in Cyp2abfgs-null mice (for both male and female mice); the rate difference was accompanied by a 5-fold higher catalytic efficiency in the TG/Cyp2abfgs-null mice and was abolished by an antibody to CYP2B6. Phenobarbital 3-5 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 348-354 25336106-6 2015 Among the cells tested, HepG2 cells were highly responsive to CYP inducers, such as 3-methylcholanthrene for CYP1A2 and phenobarbital for CYP2B6 and CYP3A4. Phenobarbital 120-133 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 138-144 20516253-6 2010 HDAC inhibitors activated the phenobarbital-responsive enhancer module of the CYP2B6 promoter in transient transfection reporter assays with Ym17 cells. Phenobarbital 30-43 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 78-84 24553381-5 2014 The results revealed that CYP1A2, CYP2B6, and CYP3A4 were induced (>2.0-fold) by omeprazole, phenobarbital, and rifampicin, respectively, in all the hepatocyte lots tested at enzyme activity level (23 lots) and mRNA level (8 lots). Phenobarbital 96-109 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 34-40 25600204-2 2015 To evaluate time-dependent cytochrome P450 induction precisely, induction of CYP1A2, CYP2B6, and CYP3A4 mRNA was confirmed (>2-fold) by the treatment with omeprazole, phenobarbital, and rifampicin, respectively, for 24 or 48 h on day 3 from the start of culture. Phenobarbital 170-183 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 85-91 24252946-5 2014 In human hepatocytes, metformin robustly suppressed the expression of CYP2B6 induced by both indirect (phenobarbital) and direct CITCO [6-(4-chlorophenyl)imidazo[2,1-b]1,3thiazole-5-carbaldehyde O-(3,4-dichlorobenzyl)oxime] activators of human CAR. Phenobarbital 103-116 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 70-76 23858795-9 2013 These results suggest that 3"-OH-CB146 is formed by PB-inducible cytochrome P450 (CYP2B enzymes) in animal and human livers and 4-OH-CB146 is a major metabolite in rat and human liver. Phenobarbital 52-54 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 82-87 21111054-4 2011 METHODS: To evaluate the xenobiotic-mediated induction of hepatocellular gene expression, the prototypical inducers rifampicin (10 muM) and phenobarbital (1 mM) were used for CYP3A4, CITCO (1 muM) and artemisinin (50 muM) were used for CYP2B6, and 3-methylcholanthrene (1 muM) and omeprazole (50 muM) were utilized for induction of CYP1A2. Phenobarbital 140-153 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 236-242 20583967-3 2010 The concentration-dependent response and time-course for the induction of CYP1A2, CYP2B6 and CYP3A4 by inducing agents beta-naphthoflavone, phenobarbital and rifampicin, respectively were examined in two or more donors using multiple end-points (mRNA, enzyme activity and Western blot analysis). Phenobarbital 140-153 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 82-88 18332078-8 2008 CYP3A4 and CYP2B6 induction in Fa2N-4 cells were also low for phenytoin, phenobarbital, and efavirenz, which are dual activators of PXR/CAR. Phenobarbital 73-86 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 11-17 18983865-6 2009 The strong and overlapping inductive role of phenobarbital strengthens the participation of CYP2B6 and CYP3A in diazepam N-demethylation and CYP3A in temazepam formation. Phenobarbital 45-58 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 92-98 20509749-3 2010 In liver microsomes from PB-treated pigs, these transcriptional activations were accompanied by an increase of various marker activities of human CYP2B6, CYP3As, CYP2C9, CYP2C19. Phenobarbital 25-27 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 146-152 20307138-6 2010 Female livers and livers exposed to inducers (phenobarbital and/or dexamethasone) were associated with higher CYP2B6. Phenobarbital 46-59 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 110-116 20019244-2 2010 In the present study, we carefully analyzed mRNA expression and activity of the major P450s and their responsiveness to three prototypical inducers, phenobarbital, rifampicin, and omeprazole, in differentiated HepaRG cell cultures over a 4-week period after low and high seeding. Phenobarbital 149-162 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 86-91 20019244-7 2010 Thus, CYP1A2, CYP2B6, and CYP3A4 were found to accurately respond to their respective prototypical inducers, i.e., omeprazole, phenobarbital, and rifampicin. Phenobarbital 127-140 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 14-20 19833192-7 2010 The results demonstrate that transcriptional activation of CYP2B6 gene is mediated mainly by the pregnane X receptor (PXR) and the Phenobarbital Responsive Element Module (PBREM). Phenobarbital 131-144 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 59-65 18474683-7 2008 OPZ also induced transcription of the human CYP2B6 promoter-reporter containing the phenobarbital (PB) responsive element in mouse liver using an in vivo transcription assay. Phenobarbital 84-97 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 44-50 18474683-7 2008 OPZ also induced transcription of the human CYP2B6 promoter-reporter containing the phenobarbital (PB) responsive element in mouse liver using an in vivo transcription assay. Phenobarbital 99-101 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 44-50 16135659-7 2005 All 23 single assays were validated by assessing the effects (induction or repression) of known inducers (ethanol, 3-methylcholanthrene, rifampicin, dexamethasone, phenobarbital) on P450 expression in human primary hepatocytes. Phenobarbital 164-177 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 182-186 18022748-6 2008 In human hepatocytes Pyrethrins and Phenobarbital induced both testosterone 6beta-hydroxylase activity and CYP3A4 mRNA levels and also increased CYP2B6 mRNA levels. Phenobarbital 36-49 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 145-151 17992728-13 2007 Kinetic analysis showed that the Km values for the formation of fenchone, 6-exo- hydroxyfenchol and 10-hydroxyfenchol in rats treated with phenobarbital were 0.06, 0.03 and 0.03 mM, and Vmax values were 2.94, 6.1 and 13.8 nmol min-1 nmol-1 P450, respectively. Phenobarbital 139-152 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 240-244 16623664-1 2006 CAR (constitutive active/androstane receptor) regulates both the distal enhancer PBREM (phenobarbital-responsive enhancer module) and the proximal element OARE [OA (okadaic acid) response element] to synergistically up-regulate the endogenous CYP2B6 (where CYP is cytochrome P450) gene in HepG2 cells. Phenobarbital 88-101 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 243-249 16623664-1 2006 CAR (constitutive active/androstane receptor) regulates both the distal enhancer PBREM (phenobarbital-responsive enhancer module) and the proximal element OARE [OA (okadaic acid) response element] to synergistically up-regulate the endogenous CYP2B6 (where CYP is cytochrome P450) gene in HepG2 cells. Phenobarbital 88-101 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 275-279 18094037-11 2008 Phenobarbital (constitutive androstane receptor activator) induced CYP3A4 (4.1-fold, only in jejunum), CYP2B6 (4.9-fold in colon and 2.3-fold in proximal jejunum), and MDR1/ABCB1 mRNA and CYP3A4 activity (2-fold only proximal jejunum). Phenobarbital 0-13 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 103-109 17904175-2 2007 Phenobarbital (PB) induction of human CYP 2B6 and mouse CYP 2b10 has been shown to be mediated by CAR. Phenobarbital 0-13 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 38-45 17904175-2 2007 Phenobarbital (PB) induction of human CYP 2B6 and mouse CYP 2b10 has been shown to be mediated by CAR. Phenobarbital 15-17 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 38-45 17638512-2 2007 One of the less well-studied human cytochrome P450s is (CYP)2B6, a homologue of the rodent phenobarbital-inducible CYP2B enzymes. Phenobarbital 91-104 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 56-63 17638512-2 2007 One of the less well-studied human cytochrome P450s is (CYP)2B6, a homologue of the rodent phenobarbital-inducible CYP2B enzymes. Phenobarbital 91-104 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 115-120 16988011-1 2006 Our previous studies have suggested a role for AMP-activated protein kinase (AMPK) in the induction of CYP2B6 by phenobarbital (PB) in hepatoma-derived cells (Rencurel et al., 2005). Phenobarbital 113-126 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 103-109 16988011-1 2006 Our previous studies have suggested a role for AMP-activated protein kinase (AMPK) in the induction of CYP2B6 by phenobarbital (PB) in hepatoma-derived cells (Rencurel et al., 2005). Phenobarbital 128-130 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 103-109 15703377-5 2005 To further validate S. cerevisiae as a model for exploring mammalian P450 turnover, the degradation of phenobarbital-inducible liver CYP2B1, an enzyme reportedly degraded via the rat hepatic autophagic-lysosomal pathway, was examined in a yeast strain (pep4delta) deficient in vacuolar degradation and its isogenic wild-type control (PEP4). Phenobarbital 103-116 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 69-73 15802384-1 2005 Constitutive active (or androstane) receptor (CAR, NR1I3), a member of the nuclear receptor family, is a major regulator for induction of cytochrome P450 2B (CYP2B) genes by phenobarbital. Phenobarbital 174-187 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 138-156 15802384-1 2005 Constitutive active (or androstane) receptor (CAR, NR1I3), a member of the nuclear receptor family, is a major regulator for induction of cytochrome P450 2B (CYP2B) genes by phenobarbital. Phenobarbital 174-187 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 158-163 15572372-0 2005 AMP-activated protein kinase mediates phenobarbital induction of CYP2B gene expression in hepatocytes and a newly derived human hepatoma cell line. Phenobarbital 38-51 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 65-70 16012075-8 2005 Phenobarbital-mediated responsiveness of cytochrome P450 2B6, a potential indicator of hepatocyte differentiation status, was markedly higher in overlaid relative to non-overlaid hepatocytes. Phenobarbital 0-13 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 41-60 15572372-9 2005 Expression of a constitutively active form of AMPK mimics the PB induction of CYP2B6 and CYP2B1 gene expression. Phenobarbital 62-64 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 78-84 15572372-12 2005 Our data strongly support a role for AMPK in the PB induction of CYP2B gene expression and provide new insights into the regulation of gene expression by barbiturate drugs. Phenobarbital 49-51 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 65-70 20021072-7 2005 In contrast, CYP2B6, the human orthologue of the rodent phenobarbital-inducible P450 2B, is known to be inducible by a range of substances, but our recent studies also show a high degree of genetic polymorphism. Phenobarbital 56-69 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 13-19 15563456-1 2005 The constitutive active receptor (CAR) regulates the induction of the cytochrome P450 2B6 (CYP2B6) gene by phenobarbital-type inducers, such as 1,4 bis[2-(3,5-dichloropyridyloxy)]benzene (TCPOBOP) via the distal phenobarbital-responsive enhancer module (PBREM, at -1732/-1685 bp). Phenobarbital 107-120 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 70-89 15563456-1 2005 The constitutive active receptor (CAR) regulates the induction of the cytochrome P450 2B6 (CYP2B6) gene by phenobarbital-type inducers, such as 1,4 bis[2-(3,5-dichloropyridyloxy)]benzene (TCPOBOP) via the distal phenobarbital-responsive enhancer module (PBREM, at -1732/-1685 bp). Phenobarbital 107-120 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 91-97 15563456-1 2005 The constitutive active receptor (CAR) regulates the induction of the cytochrome P450 2B6 (CYP2B6) gene by phenobarbital-type inducers, such as 1,4 bis[2-(3,5-dichloropyridyloxy)]benzene (TCPOBOP) via the distal phenobarbital-responsive enhancer module (PBREM, at -1732/-1685 bp). Phenobarbital 212-225 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 70-89 15563456-1 2005 The constitutive active receptor (CAR) regulates the induction of the cytochrome P450 2B6 (CYP2B6) gene by phenobarbital-type inducers, such as 1,4 bis[2-(3,5-dichloropyridyloxy)]benzene (TCPOBOP) via the distal phenobarbital-responsive enhancer module (PBREM, at -1732/-1685 bp). Phenobarbital 212-225 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 91-97 15382119-4 2004 We show that IL-1beta decreases CAR expression and decreases phenobarbital- or bilirubin-mediated induction of CYP2B6, CYP2C9, CYP3A4, UGT1A1, GSTA1, GSTA2, and SLC21A6 messenger RNA. Phenobarbital 61-74 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 111-117 14977870-7 2004 EC50 values for CYP2B6 and CYP3A4 induction by clotrimazole, phenobarbital, phenytoin, and rifampin were strongly correlated (r2 = 0.99) and were statistically indistinguishable for clotrimazole, phenytoin, and rifampin. Phenobarbital 61-74 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 16-22 15123723-4 2004 In primary human hepatocytes, expression of CYP2B6 reporter genes containing phenobarbital-responsive enhancer module (PBREM) or PBREM/xenobiotic-responsive enhancer module (XREM) response elements were activated up to 14- and 28-fold, respectively, by 50 microm PHY. Phenobarbital 77-90 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 44-50 14978227-7 2004 In the reporter gene assays employing the phenobarbital-responsible enhancer module (PBREM) from CYP2B6 and UGT1A1 genes, the splice variants, except for SV1, were inactive, whereas SV1 transactivated the CYP2B6 PBREM but not the UGT1A1 PBREM reporter. Phenobarbital 42-55 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 97-103 14978227-7 2004 In the reporter gene assays employing the phenobarbital-responsible enhancer module (PBREM) from CYP2B6 and UGT1A1 genes, the splice variants, except for SV1, were inactive, whereas SV1 transactivated the CYP2B6 PBREM but not the UGT1A1 PBREM reporter. Phenobarbital 42-55 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 205-211 12870655-7 2003 Additional experiments showed that phenobarbital increased CYP2B6 mRNA expression and that pregnane X receptor (PXR) but not constitutive androstane receptor (CAR) was detected in HL-60 cells. Phenobarbital 35-48 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 59-65 14683479-1 2003 Cytochrome p450 2B genes have been used extensively as prototypical models to study phenobarbital induction of p450 enzymes. Phenobarbital 84-97 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 11-15 14683479-1 2003 Cytochrome p450 2B genes have been used extensively as prototypical models to study phenobarbital induction of p450 enzymes. Phenobarbital 84-97 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 111-115 12815172-4 2003 Colchicine (COL) decreased both basal and rifampicin- and phenobarbital-inducible expression of CYP2B6, CYP2C8/9, and CYP3A4. Phenobarbital 58-71 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 96-102 12695351-4 2003 In contrast, treatment with 0.1 microM DEX enhanced CYP2B6 induction by different pregnane X receptor (PXR) activators, including rifampin, phenytoin, clotrimazole, and phenobarbital. Phenobarbital 169-182 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 52-58 12695351-5 2003 In Huh7 cells, cotransfection of human (h)GR and hPXR with CYP2B6-phenobarbital-responsive enhancer module (PBREM) reporter constructs revealed that all hPXR ligands induce CYP2B6 reporter gene activity, and this ligand-dependent activation is greatly enhanced by activated hGR. Phenobarbital 66-79 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 59-65 11854139-2 2002 In the present study, N-3-benzyl derivatives of nirvanol and phenobarbital were synthesized, their respective (+)- and (-)-enantiomers resolved chromatographically, and inhibitor potencies determined for these compounds toward CYP2C19 and other human liver cytochromes P450 (P450s). Phenobarbital 61-74 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 269-273 12511605-1 2003 The constitutive androstane receptor (CAR, NR1I3) transcriptionally activates cytochrome P450 2B6, 2C9, and 3A4 when activated by xenobiotics, such as phenobarbital. Phenobarbital 151-164 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 78-102 12739762-0 2003 Induction of cytochrome P450 2B6 and 3A4 expression by phenobarbital and cyclophosphamide in cultured human liver slices. Phenobarbital 55-68 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 13-32 12739762-8 2003 CYP2B6 and 3A4 mRNA, apoprotein, and enzyme-related activities were induced by phenobarbital and cyclophosphamide, whereas CYP2C9 apoprotein was not. Phenobarbital 79-92 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 0-6 11518807-4 2001 Thus, the phenobarbital-inducible enhancer units of the mouse Cyp2b10, rat CYP2B2, and human CYP2B6 genes were activated in reporter gene assays by the same compounds that activate the chicken CYP2H1 phenobarbital-inducible enhancer units. Phenobarbital 10-23 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 93-99 11502872-5 2001 PXR was shown to be capable of activating the phenobarbital-responsive enhancer module (PBREM) region of the CYP2B6 gene, a 51-base-pair enhancer element that mediates induction of CYP2B6 expression by CAR. Phenobarbital 46-59 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 109-115 11502872-5 2001 PXR was shown to be capable of activating the phenobarbital-responsive enhancer module (PBREM) region of the CYP2B6 gene, a 51-base-pair enhancer element that mediates induction of CYP2B6 expression by CAR. Phenobarbital 46-59 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 181-187 11518807-4 2001 Thus, the phenobarbital-inducible enhancer units of the mouse Cyp2b10, rat CYP2B2, and human CYP2B6 genes were activated in reporter gene assays by the same compounds that activate the chicken CYP2H1 phenobarbital-inducible enhancer units. Phenobarbital 200-213 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 93-99 11257517-7 2001 P450 enzyme activities were assessed using 8 different substrates and increases were found after treatment with phenobarbitone, rifampicin, and dioxin. Phenobarbital 112-126 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 0-4 11093784-2 2000 CAR is a member of the nuclear receptor family (NR1) mostly expressed in the liver, which heterodimerizes with retinoid X receptor (RXR) and was shown to transactivate both the phenobarbital responsive element module of the human CYP2B6 gene and the CYP3A4 xenobiotic response element. Phenobarbital 177-190 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 230-236 11181490-6 2001 The concentration dependence of CYP2C8 and CYP2C9 mRNAs in response to rifampicin and phenobarbital paralleled that of CYP3A4 and CYP2B6, the maximum accumulation being reached with 10 microM rifampicin and 100 microM phenobarbital. Phenobarbital 86-99 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 130-136 11181490-6 2001 The concentration dependence of CYP2C8 and CYP2C9 mRNAs in response to rifampicin and phenobarbital paralleled that of CYP3A4 and CYP2B6, the maximum accumulation being reached with 10 microM rifampicin and 100 microM phenobarbital. Phenobarbital 218-231 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 130-136 11231298-5 2001 Methylcholanthrene induced an increase in CYP1A1/2 enzyme activity (eightfold), phenobarbital induced CYP2B6 activity (1.7-fold), and dexamethasone induced CYP3A4 activity (fivefold). Phenobarbital 80-93 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 102-108 11093784-4 2000 We show that submicromolar concentrations of dexamethasone enhance phenobarbital-mediated induction of CYP3A4, CYP2B6, and CYP2C8 mRNA in cultured human hepatocytes. Phenobarbital 67-80 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 111-117 10788445-4 2000 This motif resembles phenobarbital response elements in the flanking regions of three phenobarbital-inducible genes, rat CYP2B2, mouse Cyp2b10, and human CYP2B6. Phenobarbital 21-34 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 154-160 10924340-4 2000 In parallel, IL-6 decreases both rifampicin- and phenobarbital-mediated induction of CYP2B6, CYP2C8, CYP2C9, and CYP3A4. Phenobarbital 49-62 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 85-91 10788445-4 2000 This motif resembles phenobarbital response elements in the flanking regions of three phenobarbital-inducible genes, rat CYP2B2, mouse Cyp2b10, and human CYP2B6. Phenobarbital 86-99 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 154-160 10729196-12 2000 Upon incubation with phenobarbital and rifampin (rifampicin), human hepatocytes increased CYP 2B6, 3A4, and 3A5 among others. Phenobarbital 21-34 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 90-97 10471061-11 1999 We also found that CYP2B6 is induced at protein and mRNA levels by phenobarbital (2 mM) and cyclophosphamide (1 mM), an anticancer drug known to be metabolized by CYP2B6. Phenobarbital 67-80 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 19-25 10471061-11 1999 We also found that CYP2B6 is induced at protein and mRNA levels by phenobarbital (2 mM) and cyclophosphamide (1 mM), an anticancer drug known to be metabolized by CYP2B6. Phenobarbital 67-80 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 163-169 2122480-0 1990 Studies on the mechanism of monoclonal antibody inhibition of enzyme activity of phenobarbital-induced cytochrome P-450. Phenobarbital 81-94 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 114-119 10037683-0 1999 The repressed nuclear receptor CAR responds to phenobarbital in activating the human CYP2B6 gene. Phenobarbital 47-60 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 85-91 10037683-1 1999 The endogenous CYP2B6 gene becomes phenobarbital (PB) inducible in androstenol-treated HepG2 cells either transiently or stably transfected with a nuclear receptor CAR expression vector. Phenobarbital 35-48 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 15-21 10037683-1 1999 The endogenous CYP2B6 gene becomes phenobarbital (PB) inducible in androstenol-treated HepG2 cells either transiently or stably transfected with a nuclear receptor CAR expression vector. Phenobarbital 50-52 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 15-21 10037683-6 1999 Thus, activation of the repressed nuclear receptor CAR appears to be a versatile mediator that regulates PB induction of the CYP2B and other genes. Phenobarbital 105-107 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 125-130 8091338-11 1993 Some P450 (3A, 1A, 2E, ...) are inducible by compounds such as phenobarbital, rifampicin, aromatic hydrocarbon, ethanol, or omeprazole. Phenobarbital 63-76 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 5-9 8395842-3 1993 Antibody inhibition experiments indicated that ethoxyresorufin and methoxyresorufin O-dealkylations were catalysed mainly by the P450 1A subfamily in untreated and BA-induced HepG2 cells, that additional unidentified P450 forms were considerably involved in methoxyresorufin and benzyloxyresorufin O-dealkylations and that the P450 2B subfamily was partially responsible for pentoxyresorufin O-dealkylation in PB-induced cells. Phenobarbital 410-412 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 129-133 2400826-1 1990 The adaptive response of the liver to phenobarbital is characterized by a strong cell hypertrophy and coordinate induction of specific P450 forms (IIB1, 2; IIC7, IIIA1). Phenobarbital 38-51 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 126-151 2337348-0 1990 Selective detection of mRNA forms encoding the major phenobarbital inducible cytochromes P450 and other members of the P450IIB family by the RNAse A protection assay. Phenobarbital 53-66 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 89-93 2122480-1 1990 Four monoclonal antibodies (MAbs) to phenobarbital-induced cytochrome P-450 (PB-P-450) show different patterns of inhibition of PB-P-450 catalyzed aryl hydrocarbon hydroxylase (AHH), 7-ethoxycoumarin deethylase, benzphetamine demethylase and ethylmorphine demethylase. Phenobarbital 37-50 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 70-75 2122480-1 1990 Four monoclonal antibodies (MAbs) to phenobarbital-induced cytochrome P-450 (PB-P-450) show different patterns of inhibition of PB-P-450 catalyzed aryl hydrocarbon hydroxylase (AHH), 7-ethoxycoumarin deethylase, benzphetamine demethylase and ethylmorphine demethylase. Phenobarbital 37-50 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 77-85 2122480-1 1990 Four monoclonal antibodies (MAbs) to phenobarbital-induced cytochrome P-450 (PB-P-450) show different patterns of inhibition of PB-P-450 catalyzed aryl hydrocarbon hydroxylase (AHH), 7-ethoxycoumarin deethylase, benzphetamine demethylase and ethylmorphine demethylase. Phenobarbital 37-50 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 128-136 2574176-2 1989 The phenobarbital-inducible P-450 forms IIB1 and IIB2 are identical in sequence except for 14 amino acid differences within the carboxyl-terminal half of the molecule. Phenobarbital 4-17 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 40-44 2540752-1 1989 The phosphorylation of the two major phenobarbital-inducible cytochrome P450 isoenzymes IIB1 and IIB2 was increased in hepatocytes by the action of the membrane permeating cAMP derivatives N6-dibutyryl-cAMP and 8-thiomethyl-cAMP. Phenobarbital 37-50 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 88-92 3050970-2 1988 The antibodies used in the immunohistochemical analyses were monoclonal antibodies (MAbs) 1-7-1 and 2-66-3, prepared against the 3-methylcholanthrene-induced and phenobarbital-induced rat liver P-450, respectively. Phenobarbital 162-175 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 194-199 2535970-0 1989 Phosphorylation of carcinogen metabolizing enzymes: regulation of the phosphorylation status of the major phenobarbital inducible cytochromes P-450 in hepatocytes. Phenobarbital 106-119 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 142-147 2535970-1 1989 We present data showing that the major phenobarbital inducible cytochromes P-450 (cytochrome P-450IIB1 and cytochrome P-450IIB2) were phosphorylated in intact hepatocytes. Phenobarbital 39-52 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 75-80 2535970-1 1989 We present data showing that the major phenobarbital inducible cytochromes P-450 (cytochrome P-450IIB1 and cytochrome P-450IIB2) were phosphorylated in intact hepatocytes. Phenobarbital 39-52 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 82-102 2963808-1 1987 P-450 human-2 is a human cytochrome P-450 that is immunochemically related to a constitutive male-specific cytochrome P-450 (P-450-male) and the phenobarbital-inducible P-450b/e in rat liver. Phenobarbital 145-158 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 0-5 2963808-1 1987 P-450 human-2 is a human cytochrome P-450 that is immunochemically related to a constitutive male-specific cytochrome P-450 (P-450-male) and the phenobarbital-inducible P-450b/e in rat liver. Phenobarbital 145-158 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 36-41 2963808-1 1987 P-450 human-2 is a human cytochrome P-450 that is immunochemically related to a constitutive male-specific cytochrome P-450 (P-450-male) and the phenobarbital-inducible P-450b/e in rat liver. Phenobarbital 145-158 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 36-41 2424910-0 1986 The cDNA and protein sequence of a phenobarbital-induced chicken cytochrome P-450. Phenobarbital 35-48 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 76-81 3690723-1 1987 Distinct and different molecular structural features are manifested by substrates, inhibitors and inducers of the two families of liver microsomal enzymes, the phenobarbital-induced cytochromes P-450 and the 3-methylcholanthrene-induced cytochromes P-448. Phenobarbital 160-173 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 194-199 3690723-4 1987 The implications are that the binding sites of cytochromes P-448 contain a number of hydrophobic aromatic amino acid residues orientated so as to allow occupation by similar substrates containing co-planar aromatic rings, whereas those of the phenobarbital-induced cytochromes P-450 contain hydrophilic amino acid residues capable of hydrogen bonding to greater than C = O moieties and at least one leucine or valine residue, as these contain the complementary isopropyl function. Phenobarbital 243-256 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 277-282 2424910-1 1986 Several cDNA clones complementary to a chicken phenobarbital-inducible cytochrome P-450 have been isolated and sequenced, representing the first non-mammalian eukaryotic cytochrome P-450 sequence to be analyzed. Phenobarbital 47-60 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 82-87 2424910-1 1986 Several cDNA clones complementary to a chicken phenobarbital-inducible cytochrome P-450 have been isolated and sequenced, representing the first non-mammalian eukaryotic cytochrome P-450 sequence to be analyzed. Phenobarbital 47-60 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 181-186 2424910-6 1986 The chicken cytochrome P-450 shows an overall homology of 45-54% compared with the mammalian phenobarbital-induced cytochrome P-450s. Phenobarbital 93-106 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 23-28 3921402-5 1985 However, phenobarbital-inducible proteins were identified on "Western blots" using antibodies to a rat liver phenobarbital inducible P-450 form. Phenobarbital 9-22 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 133-138 3921402-5 1985 However, phenobarbital-inducible proteins were identified on "Western blots" using antibodies to a rat liver phenobarbital inducible P-450 form. Phenobarbital 109-122 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 133-138 118106-5 1979 Addition of phenobarbital or methylcholanthrene at day 5 in culture caused an increase in cytochromes P-450 and P-448, respectively, only in hepatocytes cultured on collagen membranes and confluent fibroblasts. Phenobarbital 12-25 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 102-117 6438379-1 1984 Cytochrome P-450-dependent oxidative cleavage of 1-(tetrahydro-2-furanyl)-5-fluorouracil (FT) was investigated in a reconstituted system containing purified phenobarbital-inducible cytochrome P-450 (P-450(1)) or 3-methylcholanthrene-inducible cytochrome P-450 (P-448(1)). Phenobarbital 157-170 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 11-16 33551819-6 2020 As expected, CITCO, the direct activator, and PB, the indirect activator of CAR, induced CYP3A4 (31 and 40-fold), CYP2B6 (24 and 28-fold) and UGT1A1 (2.9 and 4.2-fold), respectively. Phenobarbital 46-48 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 114-120 31924695-0 2020 Ser100-Phosphorylated RORalpha Orchestrates CAR and HNF4alpha to Form Active Chromatin Complex in Response to Phenobarbital to Regulate Induction of CYP2B6. Phenobarbital 110-123 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 149-155 31924695-2 2020 Here, we found serine 100-phosphorylated RORalpha orchestrates constitutive androstane receptor (CAR) and hepatocyte nuclear factor 4 alpha (HNF4alpha) to induce CYP2B6 by phenobarbital (PB) in human primary hepatocytes (HPHs). Phenobarbital 172-185 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 162-168 31924695-2 2020 Here, we found serine 100-phosphorylated RORalpha orchestrates constitutive androstane receptor (CAR) and hepatocyte nuclear factor 4 alpha (HNF4alpha) to induce CYP2B6 by phenobarbital (PB) in human primary hepatocytes (HPHs). Phenobarbital 187-189 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 162-168 31924695-9 2020 Altogether, the results established that p-Ser100 RORalpha bridging the PBREM and OARE orchestrates CAR and HNF4alpha to form active chromatin complex during PB-induced CYP2B6 expression in human primary hepatocytes. Phenobarbital 72-74 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 169-175 31924695-10 2020 SIGNIFICANCE STATEMENT: CYP2B6 is a vital enzyme for the metabolic elimination of xenobiotics, and it is prone to induction by xenobiotics, including phenobarbital via constitutive androstane receptor (CAR) and hepatocyte nuclear factor 4 alpha (HNF4alpha). Phenobarbital 150-163 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 24-30 31270214-5 2019 In addition, the knockdown of ADAR1 resulted in the enhanced induction of CYP2B6 and CYP3A4 mRNA by 6-(4-chlorophenyl)imidazo[2,1-b][1,3]thiazole-5-carbaldehyde O-(3,4-dichlorobenzyl)oxime and phenobarbital, respectively. Phenobarbital 193-206 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 74-80 31436536-3 2019 Here, we show that in a human CAR (hCAR)-knockout (KO) HepaRG cell line, PB significantly induces the expression of CYP2B6 and CYP3A4, two shared target genes of hCAR and human PXR (hPXR). Phenobarbital 73-75 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 116-122 30592088-10 2019 Exposure to phenobarbital resulted in an approximately twofold increase in CYP 2B6 enzyme activity. Phenobarbital 12-25 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 75-82 29357810-6 2018 RESULTS: Although the activities of CYP2B6 and CYP3A were induced by treatment with PB and RIF, we found that the activity of phenacetin O-deethylase (PHOD), which is known as a marker of the activity of CYP1A2, was also enhanced by treatment with these non-CYP1A2 inducers in HepaRG cells. Phenobarbital 84-86 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 36-42