PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 21897005-1 2011 Prostate specific antigen (PSA) exhibits pronounced heterogeneity in both primary structure and glycan composition, resulting in the existence of different molecular forms. Polysaccharides 96-102 kallikrein related peptidase 3 Homo sapiens 0-31 21133419-4 2011 We demonstrate that TSA increased the sensitivity of the microarray over 100 times for glycan profiling using the model protein prostate specific antigen (PSA). Polysaccharides 87-93 kallikrein related peptidase 3 Homo sapiens 128-153 21133419-4 2011 We demonstrate that TSA increased the sensitivity of the microarray over 100 times for glycan profiling using the model protein prostate specific antigen (PSA). Polysaccharides 87-93 kallikrein related peptidase 3 Homo sapiens 155-158 21133419-5 2011 The glycan profile of PSA enriched from LNCAP cells, obtained at a subnanogram level with the aid of TSA, was consistent with the previous reports. Polysaccharides 4-10 kallikrein related peptidase 3 Homo sapiens 22-25 20726802-0 2010 Glycan characterization of PSA 2-DE subforms from serum and seminal plasma. Polysaccharides 0-6 kallikrein related peptidase 3 Homo sapiens 27-30 20726802-6 2010 Furthermore, the analysis of F3, the more abundant PSA subform, showed a higher proportion of alpha 2-3 sialic acid and a decrease in core fucosylated glycans in the PCa sample. Polysaccharides 151-158 kallikrein related peptidase 3 Homo sapiens 51-54 17956937-3 2008 In the present study, the glycan profiles of free and complexed forms of PSA from cancer patient serum and of seminal plasma PSA were compared by analyzing the glycopeptides obtained by lysylendopeptidase digestion of the electrophoretically separated PSA with mass spectrometry. Polysaccharides 26-32 kallikrein related peptidase 3 Homo sapiens 73-76 19128049-7 2009 The glycans identified in these studies will allow for more defined targeting of prostate disease-specific changes for PAP, PSA and other secreted prostatic glycoproteins. Polysaccharides 4-11 kallikrein related peptidase 3 Homo sapiens 124-127 19152248-1 2009 The present investigation was a lectin-based diagnosis of malignant prostate cancer (PC) by the interaction of phytohemagglutinin (PHA lectin) from Phaseolus vulgaris with the glycan part of serum prostate specific antigen (PSA) of patients with prostatic disorder. Polysaccharides 176-182 kallikrein related peptidase 3 Homo sapiens 197-222 19126965-7 2008 In this review, we focus on the glycan changes of two serum glycoproteins, prostate specific antigen--currently used as a tumour marker of prostate cancer--and human pancreatic ribonuclease in pancreatic adenocarcinoma. Polysaccharides 32-38 kallikrein related peptidase 3 Homo sapiens 75-100 12626390-5 2003 Glycans from normal PSA (that correspond to low and high pI PSA fractions) were sialylated biantennary complex structures, half of them being disialylated in the low pI PSA fraction and mostly monosialylated in the high pI PSA. Polysaccharides 0-7 kallikrein related peptidase 3 Homo sapiens 20-23 16177264-0 2006 Different glycan structures in prostate-specific antigen from prostate cancer sera in relation to seminal plasma PSA. Polysaccharides 10-16 kallikrein related peptidase 3 Homo sapiens 113-116 16177264-4 2006 We therefore undertook a detailed glycan analysis of PSA derived from sera from PCa patients and, importantly, established that the glycosylation of the PCa serum PSA was significantly different from the PSA from the LNCaP cell line. Polysaccharides 34-40 kallikrein related peptidase 3 Homo sapiens 163-166 16177264-4 2006 We therefore undertook a detailed glycan analysis of PSA derived from sera from PCa patients and, importantly, established that the glycosylation of the PCa serum PSA was significantly different from the PSA from the LNCaP cell line. Polysaccharides 34-40 kallikrein related peptidase 3 Homo sapiens 163-166 12626390-6 2003 PSA from LNCaP cells was purified to homogeneity, and its glycan analysis showed a significantly different pattern, especially in the outer ends of the biantennary complex structures. Polysaccharides 58-64 kallikrein related peptidase 3 Homo sapiens 0-3 14733546-1 2004 Chemical syntheses are reported for prostate specific antigen (PSA) N-linked glycopeptide fragments consisting of an uneicosapeptide (residues 27-47 of PSA) with di-, tri-, and tetrabranched N-acetyllactosamine-type glycans. Polysaccharides 216-223 kallikrein related peptidase 3 Homo sapiens 36-61 14733546-1 2004 Chemical syntheses are reported for prostate specific antigen (PSA) N-linked glycopeptide fragments consisting of an uneicosapeptide (residues 27-47 of PSA) with di-, tri-, and tetrabranched N-acetyllactosamine-type glycans. Polysaccharides 216-223 kallikrein related peptidase 3 Homo sapiens 63-66 12626390-5 2003 Glycans from normal PSA (that correspond to low and high pI PSA fractions) were sialylated biantennary complex structures, half of them being disialylated in the low pI PSA fraction and mostly monosialylated in the high pI PSA. Polysaccharides 0-7 kallikrein related peptidase 3 Homo sapiens 60-63 12626390-5 2003 Glycans from normal PSA (that correspond to low and high pI PSA fractions) were sialylated biantennary complex structures, half of them being disialylated in the low pI PSA fraction and mostly monosialylated in the high pI PSA. Polysaccharides 0-7 kallikrein related peptidase 3 Homo sapiens 60-63 12626390-5 2003 Glycans from normal PSA (that correspond to low and high pI PSA fractions) were sialylated biantennary complex structures, half of them being disialylated in the low pI PSA fraction and mostly monosialylated in the high pI PSA. Polysaccharides 0-7 kallikrein related peptidase 3 Homo sapiens 60-63 32816187-10 2021 Our results demonstrated that a multiplex diagnostic combining G81 glycan-binding auto-IgG, GDF-15/NAG-1 and PSA (>= 2.1 ng PSA/ml for cancer) increased the specificity of prostate cancer diagnosis by 8%. Polysaccharides 67-73 kallikrein related peptidase 3 Homo sapiens 124-127 12385925-1 2002 OBJECTIVES: To determine whether supplemental amounts of a polysaccharide/oligosaccharide complex obtained from a shiitake mushroom extract (SME) would lower the prostate-specific antigen (PSA) level in patients with prostate cancer. Polysaccharides 59-73 kallikrein related peptidase 3 Homo sapiens 189-192 34625256-3 2021 In this paper we report on the development and implementation of a high-throughput capillary electrophoresis based glycan analysis workflow for urinary PSA analysis. Polysaccharides 115-121 kallikrein related peptidase 3 Homo sapiens 152-155 33288424-0 2021 Impedimetric aptamer-based glycan PSA score for discrimination of prostate cancer from other prostate diseases. Polysaccharides 27-33 kallikrein related peptidase 3 Homo sapiens 34-37 33288424-8 2021 The final output of the proposed platform is the ratio between PSAG-1 reactive PSA and total PSA, defined as the glycan score. Polysaccharides 113-119 kallikrein related peptidase 3 Homo sapiens 63-66 30640124-0 2019 Focusing aptamer selection on the glycan structure of prostate-specific antigen: Toward more specific detection of prostate cancer. Polysaccharides 34-40 kallikrein related peptidase 3 Homo sapiens 54-79 30640124-3 2019 In this work, we describe a simple method for directing the selection of aptamers toward the glycan structure of the glycoproteins, with prostate-specific antigen (PSA) as a model target. Polysaccharides 93-99 kallikrein related peptidase 3 Homo sapiens 137-168 30640124-4 2019 Using this strategy, we identified one aptamer (PSA-1) that binds the glycan moiety of PSA with reasonable affinity (a dissociation constant of 177 +- 65 nM). Polysaccharides 70-76 kallikrein related peptidase 3 Homo sapiens 48-51 30640124-4 2019 Using this strategy, we identified one aptamer (PSA-1) that binds the glycan moiety of PSA with reasonable affinity (a dissociation constant of 177 +- 65 nM). Polysaccharides 70-76 kallikrein related peptidase 3 Homo sapiens 87-90 30640124-7 2019 Although validation on a larger cohort is needed, this is the first demonstration of an aptamer-based sensor to detect PSA by focusing in its glycan moiety. Polysaccharides 142-148 kallikrein related peptidase 3 Homo sapiens 119-122 30380399-5 2019 Mass spectrometry analysis focussed on the identification of proteins carrying glycans with immunomodulatory epitopes, including fibronectin, lactoferrin, clusterin, zinc-alpha2-glycoprotein, prostate acid phosphatase and prostate-specific antigen; these should be submitted to further detailed analysis. Polysaccharides 79-86 kallikrein related peptidase 3 Homo sapiens 222-247 29100363-5 2017 In this study, we purified PSA from expressed prostate secretions (EPS)-urine samples from 32 BPH and 30 PCa patients and provided detailed PSA glycan profiles in Chinese population. Polysaccharides 144-150 kallikrein related peptidase 3 Homo sapiens 27-30 29104305-1 2017 The main aim of the research was to design a functional impedimetric biosensor able to glycoprofile prostate specific antigen (PSA), a biomarker for prostate cancer (PCa), with high specificity using lectins as glycan recognising proteins. Polysaccharides 211-217 kallikrein related peptidase 3 Homo sapiens 100-125 29104305-1 2017 The main aim of the research was to design a functional impedimetric biosensor able to glycoprofile prostate specific antigen (PSA), a biomarker for prostate cancer (PCa), with high specificity using lectins as glycan recognising proteins. Polysaccharides 211-217 kallikrein related peptidase 3 Homo sapiens 127-130 29100363-6 2017 We found that most of the PSA glycans from EPS-urine were complex type biantennary glycans. Polysaccharides 30-37 kallikrein related peptidase 3 Homo sapiens 26-29 29100363-6 2017 We found that most of the PSA glycans from EPS-urine were complex type biantennary glycans. Polysaccharides 83-90 kallikrein related peptidase 3 Homo sapiens 26-29 29100363-7 2017 We observed two major patterns in PSA glycan profiles. Polysaccharides 38-44 kallikrein related peptidase 3 Homo sapiens 34-37 28134773-0 2017 Wisteria floribunda Agglutinin and Its Reactive-Glycan-Carrying Prostate-Specific Antigen as a Novel Diagnostic and Prognostic Marker of Prostate Cancer. Polysaccharides 48-54 kallikrein related peptidase 3 Homo sapiens 64-89 28495148-5 2017 Although urine is a simpler matrix for analyzing PSA glycosylation compared to serum, an immunopurification step was necessary to specifically detect the glycans on the PSA molecule. Polysaccharides 154-161 kallikrein related peptidase 3 Homo sapiens 169-172 29109592-6 2017 It also covers electrochemical methods based on the enzyme-like activity of PSA, on DNA-, aptamer- and biofuel cell-based methods, and on the detection of PSA via its glycan part. Polysaccharides 167-173 kallikrein related peptidase 3 Homo sapiens 155-158 26920336-3 2016 The biosensor showed presence of alpha-L-fucose and alpha-(2,6)-linked terminal sialic acid within PSA s glycan with high abundance, while only traces of alpha-(2,3)-linked terminal sialic acid were found. Polysaccharides 105-111 kallikrein related peptidase 3 Homo sapiens 99-102 26840176-0 2016 Development of glycan specific lectin based immunoassay for detection of prostate specific antigen. Polysaccharides 15-21 kallikrein related peptidase 3 Homo sapiens 73-98 24854630-8 2014 ELISA using PSA, PAP, and MUC1 capture antibodies and a specific core 2 O-linked sLe(x) detection antibody demonstrated elevation of this glycan structure in cancer compared to normal tissues for MUC1 (P = 0.01), PSA (P = 0.03) and near significant differences in PAP sLe(x) levels (P = 0.06). Polysaccharides 138-144 kallikrein related peptidase 3 Homo sapiens 12-15 27065039-1 2016 Glycans of prostate-specific antigen (PSA) in prostate cancer were found to be different from that in benign disease. Polysaccharides 0-7 kallikrein related peptidase 3 Homo sapiens 11-42 25327667-5 2014 PSA has a single glycosylation site at Asn69, with glycans constituting approximately 8% of the protein by weight. Polysaccharides 51-58 kallikrein related peptidase 3 Homo sapiens 0-3 26647853-2 2016 The biosensor exhibits three unique characteristics: (1) analysis of PSA with limit of detection (LOD) down to 4 aM; (2) analysis of the glycan part of PSA with LOD down to 4 aM level and; (3) both assays (i.e., PSA quantification and PSA glycoprofiling) can be performed on the same interface due to label-free analysis. Polysaccharides 137-143 kallikrein related peptidase 3 Homo sapiens 152-155 26647853-2 2016 The biosensor exhibits three unique characteristics: (1) analysis of PSA with limit of detection (LOD) down to 4 aM; (2) analysis of the glycan part of PSA with LOD down to 4 aM level and; (3) both assays (i.e., PSA quantification and PSA glycoprofiling) can be performed on the same interface due to label-free analysis. Polysaccharides 137-143 kallikrein related peptidase 3 Homo sapiens 152-155 26647853-2 2016 The biosensor exhibits three unique characteristics: (1) analysis of PSA with limit of detection (LOD) down to 4 aM; (2) analysis of the glycan part of PSA with LOD down to 4 aM level and; (3) both assays (i.e., PSA quantification and PSA glycoprofiling) can be performed on the same interface due to label-free analysis. Polysaccharides 137-143 kallikrein related peptidase 3 Homo sapiens 152-155 25727153-4 2015 The single site of N-glycosylation on PSA has been the target of multiple glycan characterization studies. Polysaccharides 74-80 kallikrein related peptidase 3 Homo sapiens 38-41 24854630-8 2014 ELISA using PSA, PAP, and MUC1 capture antibodies and a specific core 2 O-linked sLe(x) detection antibody demonstrated elevation of this glycan structure in cancer compared to normal tissues for MUC1 (P = 0.01), PSA (P = 0.03) and near significant differences in PAP sLe(x) levels (P = 0.06). Polysaccharides 138-144 kallikrein related peptidase 3 Homo sapiens 213-216 24393138-0 2014 Glycan analysis of Prostate Specific Antigen (PSA) directly from the intact glycoprotein by HR-ESI/TOF-MS. Glycans are important modulators of the biological function of proteins and are normally characterized from proteolytic glycopeptides or from (N-)glycans released enzymatically by glycosidase treatment or chemically by hydrazinolysis. Polysaccharides 0-6 kallikrein related peptidase 3 Homo sapiens 19-44 24393138-0 2014 Glycan analysis of Prostate Specific Antigen (PSA) directly from the intact glycoprotein by HR-ESI/TOF-MS. Glycans are important modulators of the biological function of proteins and are normally characterized from proteolytic glycopeptides or from (N-)glycans released enzymatically by glycosidase treatment or chemically by hydrazinolysis. Polysaccharides 0-6 kallikrein related peptidase 3 Homo sapiens 46-49 24393138-0 2014 Glycan analysis of Prostate Specific Antigen (PSA) directly from the intact glycoprotein by HR-ESI/TOF-MS. Glycans are important modulators of the biological function of proteins and are normally characterized from proteolytic glycopeptides or from (N-)glycans released enzymatically by glycosidase treatment or chemically by hydrazinolysis. Polysaccharides 107-114 kallikrein related peptidase 3 Homo sapiens 19-44 24393138-0 2014 Glycan analysis of Prostate Specific Antigen (PSA) directly from the intact glycoprotein by HR-ESI/TOF-MS. Glycans are important modulators of the biological function of proteins and are normally characterized from proteolytic glycopeptides or from (N-)glycans released enzymatically by glycosidase treatment or chemically by hydrazinolysis. Polysaccharides 107-114 kallikrein related peptidase 3 Homo sapiens 46-49 24393138-2 2014 Interpretation of isotopically resolved mass spectra of prostate specific antigen (PSA) using bioinformatics tools gives within a few hours the glycan compositions of 38 glycoforms. Polysaccharides 144-150 kallikrein related peptidase 3 Homo sapiens 56-81 24393138-2 2014 Interpretation of isotopically resolved mass spectra of prostate specific antigen (PSA) using bioinformatics tools gives within a few hours the glycan compositions of 38 glycoforms. Polysaccharides 144-150 kallikrein related peptidase 3 Homo sapiens 83-86 23764502-8 2013 The glycans on PSA are believed to be biantennary N-linked, and it has been observed that prostate cancer tissues and cell lines contain more antennae than their benign counterparts. Polysaccharides 4-11 kallikrein related peptidase 3 Homo sapiens 15-18 24367138-9 2013 Characteristic PCa PSA-IgM glycoforms pose the question of the possible role of glycosylation as a framework for immune surveillance and may be of interest in light of recent data indicating mannose-containing glycans as cancer biomarker. Polysaccharides 210-217 kallikrein related peptidase 3 Homo sapiens 19-22