PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 35486871-11 2022 Mucin glycomic analysis revealed significantly more sialylated glycans in CF and the total abundance of non-sulfated O-glycans was correlated with the relative abundance of pathogens. Polysaccharides 63-70 LOC100508689 Homo sapiens 0-5 34217596-2 2022 An important factor in this symbiosis is the interplay between microbes and human-produced glycans in mucin and breast milk. Polysaccharides 91-98 LOC100508689 Homo sapiens 102-107 34803391-4 2021 Mucin-type O-glycans alter the diversity of gastrointestinal microorganisms, which in turn increases the level of O-glycosylation of host intestinal proteins via the utilization of glycans. Polysaccharides 181-188 LOC100508689 Homo sapiens 0-5 34939101-4 2022 Mucin glycan degradation is a complex process that requires a broad range of glycan degrading enzymes, as mucin glycans are intricate and diverse molecules. Polysaccharides 77-83 LOC100508689 Homo sapiens 0-5 34939101-4 2022 Mucin glycan degradation is a complex process that requires a broad range of glycan degrading enzymes, as mucin glycans are intricate and diverse molecules. Polysaccharides 77-83 LOC100508689 Homo sapiens 106-111 34576978-1 2021 Mucin-type O-glycosylation involves the attachment of glycans to an initial O-linked N-acetylgalactosamine (GalNAc) on serine and threonine residues on proteins. Polysaccharides 54-61 LOC100508689 Homo sapiens 0-5 34307449-6 2021 Glycans including sialic acid, gangliosides, histo-blood group antigens (HBGAs), and mucin cores have been reported to interact with RV VP8*s. The glycan binding specificities of VP8*s of different RV genotypes have been studied. Polysaccharides 0-7 LOC100508689 Homo sapiens 85-90 34117223-5 2021 A total of 83 O-GalNAc glycans presenting various natural glycan epitopes are obtained and used to generate a unique synthetic mucin O-glycan microarray. Polysaccharides 58-64 LOC100508689 Homo sapiens 127-132 35487177-1 2022 For the functional analysis of mucin related glycan, we synthesized core 3 and 5 structures of mucin type O-glycan and investigated their binding interaction with lectins using sugar chip technology. Polysaccharides 45-51 LOC100508689 Homo sapiens 31-36 35487177-1 2022 For the functional analysis of mucin related glycan, we synthesized core 3 and 5 structures of mucin type O-glycan and investigated their binding interaction with lectins using sugar chip technology. Polysaccharides 45-51 LOC100508689 Homo sapiens 95-100 1718585-7 1991 This is likely to be a core glycan since 3E1.2 reacts after treatment of the mucin with trifluoromethanesulfonic acid, which removes most backbone and peripheral carbohydrates. Polysaccharides 28-34 LOC100508689 Homo sapiens 77-82 1663364-7 1991 Indeed, the increased reactivity after sialidase treatment of ovine submaxillary mucin suggests the lectin reacts with peptide-linked N-acetylgalactosamine (GalNAc), since more than 98% of the glycan chains attached to this mucin are sialylated GalNAc. Polysaccharides 193-199 LOC100508689 Homo sapiens 81-86 1663364-8 1991 The binding of SSA-M to sialidase-treated porcine mucin was inhibited strongly by GalNAc and disaccharides containing galactose (lactose, melibiose, and N-acetyllactosamine) but not by free galactose (Gal), suggesting that the glycan for optimum binding is Gal beta(1-3)GalNAc. Polysaccharides 227-233 LOC100508689 Homo sapiens 50-55 34993358-0 2021 Mucin-mimetic glycan arrays integrating machine learning for analyzing receptor pattern recognition by influenza A viruses. Polysaccharides 14-20 LOC100508689 Homo sapiens 0-5 6093693-6 1984 The mucin fermenters isolated by enrichment had a very restricted ability to utilize complex polysaccharides and their constituent monosaccharides, suggesting that the presence of plant polysaccharides in the human colon is unlikely to prevent the use of colonic mucin as an energy source by bacteria. Polysaccharides 93-108 LOC100508689 Homo sapiens 4-9 3214155-12 1988 These data provide further evidence that sulfated polysaccharides such as mucin may be a source of sulfate for SRB in the human large gut. Polysaccharides 50-65 LOC100508689 Homo sapiens 74-79 6093693-6 1984 The mucin fermenters isolated by enrichment had a very restricted ability to utilize complex polysaccharides and their constituent monosaccharides, suggesting that the presence of plant polysaccharides in the human colon is unlikely to prevent the use of colonic mucin as an energy source by bacteria. Polysaccharides 186-201 LOC100508689 Homo sapiens 4-9 857775-2 1977 It has been shown that in its histochemical properties the secreted mucin is similar to mucous secretions of the enterodermal lining of the mammalian stomach and contains neutral polysaccharides, sulfo- and slilosaccharides. Polysaccharides 179-194 LOC100508689 Homo sapiens 68-73 18867702-0 1948 Bacterial hydrolysis and utilization of polysaccharide-like substances, mucin, in saliva. Polysaccharides 40-54 LOC100508689 Homo sapiens 72-77 13861311-0 1961 The effect of a sulphated polysaccharide upon the diffusion of pepsin through mucin. Polysaccharides 26-40 LOC100508689 Homo sapiens 78-83 46329-10 1975 The basic structure of these polysaccharides is thus similar to that of normal sulfated mucin. Polysaccharides 29-44 LOC100508689 Homo sapiens 88-93 33978739-6 2021 Our results provide new knowledge on unique glycan-binding specificities for the immune-receptor Dectin-1 towards beta-glucans and the interaction of rotavirus P[19] adhesive protein with mucin O-glycan cores. Polysaccharides 44-50 LOC100508689 Homo sapiens 188-193 34056092-6 2021 To test this hypothesis, we developed synthetic mucin mimics that recapitulate the dense display of glycans and morphology of mucin. Polysaccharides 100-107 LOC100508689 Homo sapiens 48-53 33087860-3 2021 Host-derived mucus glycans on gut-secreted mucin proteins serve as a continuous endogenous source of MACs for resident microbes; here we investigate the potential role of purified, orally administered mucus glycans in maintaining a healthy microbial community. Polysaccharides 19-26 LOC100508689 Homo sapiens 43-48 33090801-2 2020 However, this barrier is constantly challenged by mucin-degrading enzymes, which tend to target anionic glycan chains such as sulfate groups and sialic acid residues. Polysaccharides 104-110 LOC100508689 Homo sapiens 50-55 33295603-4 2021 Some of these limitations stem from the difficulty to track the biosynthetic process of mucin-type O-glycosylation, especially when glycans occur in dense clusters in repeat regions of proteins, such as the mucins or IgA1. Polysaccharides 132-139 LOC100508689 Homo sapiens 88-93 33346954-4 2020 This review is focused on the development of methodologies for preparing mucin-inspired synthetic oligomers and glycopolymers, including solid-phase synthesis, polymerization of glycosylated monomers, and post-polymerization grafting of glycans to polymer chains. Polysaccharides 237-244 LOC100508689 Homo sapiens 73-78 33090801-6 2020 Our results contribute to a better understanding on how different glycans contribute to the broad spectrum of functions mucin glycoproteins have. Polysaccharides 66-73 LOC100508689 Homo sapiens 120-125 33106676-4 2020 Beyond the mucin-type O-glycopeptides discussed here, O-Pair Search also accepts user-defined glycan databases, making it compatible with many types of O-glycosylation. Polysaccharides 94-100 LOC100508689 Homo sapiens 11-16 32817557-4 2020 Here, we present a panel of bacterial proteases that cleave mucin domains via distinct peptide- and glycan-based motifs, generating a diverse enzymatic toolkit for mucin-selective proteolysis. Polysaccharides 100-106 LOC100508689 Homo sapiens 60-65 32817557-4 2020 Here, we present a panel of bacterial proteases that cleave mucin domains via distinct peptide- and glycan-based motifs, generating a diverse enzymatic toolkit for mucin-selective proteolysis. Polysaccharides 100-106 LOC100508689 Homo sapiens 164-169 30910957-4 2019 Here, we demonstrate that secreted protease of C1 esterase inhibitor (StcE), a bacterial protease from Escherichia coli, cleaves mucin domains by recognizing a discrete peptide- and glycan-based motif. Polysaccharides 182-188 LOC100508689 Homo sapiens 129-134 31991210-8 2020 To check a potential function of the enzymes for the degradation of mucosal glycan structures, porcine stomach mucin was applied as a model substrate. Polysaccharides 76-82 LOC100508689 Homo sapiens 111-116 31275257-4 2019 In this study, we used functional metagenomics to identify new metabolic pathways in uncultured bacteria involved in harvesting mucin glycans. Polysaccharides 134-141 LOC100508689 Homo sapiens 128-133 32620774-4 2020 The HMO pathways, which include enzymes with a previously unknown structural fold and specificity, were upregulated together with additional glycan-utilization loci during growth on selected HMOs and in co-cultures with Akkermansia muciniphila on mucin, suggesting an additional role in enabling cross-feeding and access to mucin O-glycans. Polysaccharides 141-147 LOC100508689 Homo sapiens 232-237 32620774-4 2020 The HMO pathways, which include enzymes with a previously unknown structural fold and specificity, were upregulated together with additional glycan-utilization loci during growth on selected HMOs and in co-cultures with Akkermansia muciniphila on mucin, suggesting an additional role in enabling cross-feeding and access to mucin O-glycans. Polysaccharides 141-147 LOC100508689 Homo sapiens 247-252 32243763-5 2020 The host"s conversion of MUC2 to the outer mucus layer allows bacteria to degrade the mucin glycans and recover the energy content that is then shared with the host. Polysaccharides 92-99 LOC100508689 Homo sapiens 86-91 31900725-4 2020 The glycans mediated binding to these cells was effectively blocked by mucin and fetuin, exhibiting 97% and 94% inhibition respectively. Polysaccharides 4-11 LOC100508689 Homo sapiens 71-76 31313786-3 2019 Here, we report the synthesis of PEG-based glycopolymers with tunable glycan composition, which approximate the extended architecture of mucin glycoproteins, and tether them to the plasma membranes of red blood cells (RBC) to construct an artificial mucin brush-like glycocalyx. Polysaccharides 70-76 LOC100508689 Homo sapiens 137-142 30296390-4 2018 Finally, mucin glycans are critical for regulating microbial interactions, serving as receptor binding sites for adhesion, as nutrient sources, and as environmental signals. Polysaccharides 15-22 LOC100508689 Homo sapiens 9-14 30792861-7 2019 In return, the glycan repertoire of mucins can select for distinct mucosa-associated bacteria that are able to bind or degrade specific mucin glycans as a nutrient source. Polysaccharides 15-21 LOC100508689 Homo sapiens 36-41 30792861-7 2019 In return, the glycan repertoire of mucins can select for distinct mucosa-associated bacteria that are able to bind or degrade specific mucin glycans as a nutrient source. Polysaccharides 142-149 LOC100508689 Homo sapiens 36-41 30338216-7 2018 Previously performed untargeted nano-high-performance liquid chromatography-chip/time-of-flight mass spectrometry was used to detect and quantify glycans originating from colonic mucin. Polysaccharides 146-153 LOC100508689 Homo sapiens 179-184 30338216-8 2018 Colonic mucin-derived O-glycans from control infants composed 37.68% (+- 3.14% SD) of the total glycan structure pool, whereas colonic mucin-derived O-glycans made up of only 1.78% (+- 0.038% SD) of the total in B. infantis EVC001 samples. Polysaccharides 24-30 LOC100508689 Homo sapiens 8-13 30338216-9 2018 The relative abundance of these colonic mucin-derived O-glycans in the total glycan pool was higher among control, 26.98% (+- 8.48% SD), relative to B. infantis-colonized infants, 1.68% (+- 1.12% SD). Polysaccharides 56-62 LOC100508689 Homo sapiens 40-45 29787815-8 2018 SiaBb1 may assist in the degradation of mucin glycan. Polysaccharides 46-52 LOC100508689 Homo sapiens 40-45 29903935-1 2018 Changes in mucin-type O-linked glycosylation are seen in over 90% of breast cancers where increased sialylation is often observed and a change from branched glycans to linear glycans is often seen. Polysaccharides 157-164 LOC100508689 Homo sapiens 11-16 29903935-1 2018 Changes in mucin-type O-linked glycosylation are seen in over 90% of breast cancers where increased sialylation is often observed and a change from branched glycans to linear glycans is often seen. Polysaccharides 175-182 LOC100508689 Homo sapiens 11-16 29720519-2 2018 In this study, we characterized the glycan binding specificities of human and porcine P[6]/P[19] RV VP8*s and found that the P[II] genogroup RV VP8*s could commonly interact with mucin core 2, which may play an important role in RV evolution and cross-species transmission. Polysaccharides 36-42 LOC100508689 Homo sapiens 179-184 29802217-8 2018 Glycosylation is a defining process for mucins that is specific with respect to additions of glycans to mucin apoprotein backbones, and glycan additions influence the physical properties of the mucins via structural modifications as well as charge interactions. Polysaccharides 93-100 LOC100508689 Homo sapiens 40-45 29802217-8 2018 Glycosylation is a defining process for mucins that is specific with respect to additions of glycans to mucin apoprotein backbones, and glycan additions influence the physical properties of the mucins via structural modifications as well as charge interactions. Polysaccharides 93-99 LOC100508689 Homo sapiens 40-45 29761603-0 2018 A "Sticky" Mucin-Inspired DNA-Polysaccharide Binder for Silicon and Silicon-Graphite Blended Anodes in Lithium-Ion Batteries. Polysaccharides 30-44 LOC100508689 Homo sapiens 11-16 28814130-0 2017 Identification and characterization of a sulfoglycosidase from Bifidobacterium bifidum implicated in mucin glycan utilization. Polysaccharides 107-113 LOC100508689 Homo sapiens 101-106 29401627-2 2018 We used a combination of structural, biochemical, biophysical, and cell-based approaches to decipher the specificity of the interaction between mucin glycans and mammalian lectins expressed in the gut, including galectin (Gal)-3 and C-type lectin receptors. Polysaccharides 150-157 LOC100508689 Homo sapiens 144-149 29401627-4 2018 Modification of mucin glycosylation, via chemical treatment leading to a loss of terminal glycans, promoted the interaction of Gal-3 to poly- N-acetyllactosamine. Polysaccharides 90-97 LOC100508689 Homo sapiens 16-21 28721502-1 2018 Mucus production is initiated before birth and provides mucin glycans to the infant gut microbiota. Polysaccharides 62-69 LOC100508689 Homo sapiens 56-61 29581231-0 2018 Intestinal mucin activates human dendritic cells and IL-8 production in a glycan-specific manner. Polysaccharides 74-80 LOC100508689 Homo sapiens 11-16 29581231-8 2018 Instead, mucin glycans are important for the pro-inflammatory effects on DCs. Polysaccharides 15-22 LOC100508689 Homo sapiens 9-14 28814130-2 2017 However, our knowledge about mucin type O-glycan degradation by bifidobacteria remains fragmentary, especially regarding how they decompose sulfated glycans, which are abundantly found in mucin sugar-chains. Polysaccharides 149-156 LOC100508689 Homo sapiens 29-34 28814130-2 2017 However, our knowledge about mucin type O-glycan degradation by bifidobacteria remains fragmentary, especially regarding how they decompose sulfated glycans, which are abundantly found in mucin sugar-chains. Polysaccharides 149-156 LOC100508689 Homo sapiens 188-193 28814130-6 2017 This de-capping activity may promote utilization of sulfated glycans of mucin by other bacteria including bifidobacteria, thereby establishing the symbiotic relationship between human and gut microbes. Polysaccharides 61-68 LOC100508689 Homo sapiens 72-77 25124036-9 2014 Supposedly, lectin TFF2 is involved in protection of gastric epithelia via a functional relationship to defense against H. pylori launched by antibiotic alpha1,4-GlcNAc-capped mucin glycans. Polysaccharides 182-189 LOC100508689 Homo sapiens 176-181 28461410-0 2017 Structural Diversity of Human Gastric Mucin Glycans. Polysaccharides 44-51 LOC100508689 Homo sapiens 38-43 26318196-0 2015 Pathobiological implications of mucin glycans in cancer: Sweet poison and novel targets. Polysaccharides 38-45 LOC100508689 Homo sapiens 32-37 26318196-7 2015 Finally, this review discusses the scope of mucin glycan epitopes as potential diagnostic and therapeutic targets. Polysaccharides 50-56 LOC100508689 Homo sapiens 44-49 26556271-4 2015 Experiments in which mucin glycans were presented simultaneously with other carbohydrates show that degradation of these host carbohydrates is consistently repressed in the presence of alternative substrates, even by B. thetaiotaomicron previously acclimated to growth in pure mucin glycans. Polysaccharides 27-34 LOC100508689 Homo sapiens 21-26 26556271-4 2015 Experiments in which mucin glycans were presented simultaneously with other carbohydrates show that degradation of these host carbohydrates is consistently repressed in the presence of alternative substrates, even by B. thetaiotaomicron previously acclimated to growth in pure mucin glycans. Polysaccharides 283-290 LOC100508689 Homo sapiens 21-26 26556271-5 2015 Experiments with media containing systematically varied carbohydrate cues and genetic mutants reveal that transcriptional repression of genes involved in mucin glycan metabolism is imposed by simple sugars and, in one example that was tested, is mediated through a small intergenic region in a transcript-autonomous fashion. Polysaccharides 160-166 LOC100508689 Homo sapiens 154-159 26556271-6 2015 Repression of mucin glycan-responsive gene clusters in two other human gut bacteria, Bacteroides massiliensis and Bacteroides fragilis, exhibited variable and sometimes reciprocal responses compared to those of B. thetaiotaomicron, revealing that these symbionts vary in their preference for mucin glycans and that these differences occur at the level of controlling individual gene clusters. Polysaccharides 20-26 LOC100508689 Homo sapiens 14-19 26556271-6 2015 Repression of mucin glycan-responsive gene clusters in two other human gut bacteria, Bacteroides massiliensis and Bacteroides fragilis, exhibited variable and sometimes reciprocal responses compared to those of B. thetaiotaomicron, revealing that these symbionts vary in their preference for mucin glycans and that these differences occur at the level of controlling individual gene clusters. Polysaccharides 20-26 LOC100508689 Homo sapiens 292-297 26556271-6 2015 Repression of mucin glycan-responsive gene clusters in two other human gut bacteria, Bacteroides massiliensis and Bacteroides fragilis, exhibited variable and sometimes reciprocal responses compared to those of B. thetaiotaomicron, revealing that these symbionts vary in their preference for mucin glycans and that these differences occur at the level of controlling individual gene clusters. Polysaccharides 298-305 LOC100508689 Homo sapiens 14-19 25951175-4 2015 These glycans can be carried on many mucin-type glycoproteins including MUC1. Polysaccharides 6-13 LOC100508689 Homo sapiens 37-42 25852737-0 2015 Mucin glycan foraging in the human gut microbiome. Polysaccharides 6-12 LOC100508689 Homo sapiens 0-5 25727146-1 2015 Mucin-type O-glycans are a class of glycans initiated with N-acetylgalactosamine (GalNAc) alpha-linked primarily to Ser/Thr residues within glycoproteins and often extended or branched by sugars or saccharides. Polysaccharides 13-20 LOC100508689 Homo sapiens 0-5 28289177-0 2017 Structural diversity of human gastric mucin glycans. Polysaccharides 44-51 LOC100508689 Homo sapiens 38-43 28091546-5 2017 We show that several pathways for targeting O-mucin glycans are activated in B. thetaiotaomicron by GOS, as well as the galactan utilization sytem. Polysaccharides 52-59 LOC100508689 Homo sapiens 46-51 28091546-6 2017 Characterization of the endo-galactanase from this system identified activity on various longer GOS substrates while a subset of GOS compounds were identified as potential activators of mucin glycan metabolism in B. thetaiotaomicron. Polysaccharides 192-198 LOC100508689 Homo sapiens 186-191 28091546-7 2017 Our results show that GOS functions as an inducer of mucin-glycan pathways while providing a nutrient source in the form of beta-(1 4)-galactan. Polysaccharides 59-65 LOC100508689 Homo sapiens 53-58 27873468-0 2017 Synthetic Mucin-Like Glycopeptides as Versatile Tools to Measure Effects of Glycan Structure/Density/Position on the Interaction with Adhesion/Growth-Regulatory Galectins in Arrays. Polysaccharides 76-82 LOC100508689 Homo sapiens 10-15 25852737-8 2015 This is largely due to the limited number of functionally characterized enzymes and the lack of studies correlating the specificity of these enzymes with the ability of the strain to degrade and utilize mucin and mucin glycans. Polysaccharides 219-226 LOC100508689 Homo sapiens 213-218 25852737-9 2015 This review focuses on recent findings unraveling the molecular strategies used by mucin-degrading bacteria to utilize host glycans, adapt to the mucosal environment, and influence human health. Polysaccharides 124-131 LOC100508689 Homo sapiens 83-88 25124036-0 2014 Human trefoil factor 2 is a lectin that binds alpha-GlcNAc-capped mucin glycans with antibiotic activity against Helicobacter pylori. Polysaccharides 72-79 LOC100508689 Homo sapiens 66-71 25106027-3 2014 Mucin glycans act as binding sites or carbon sources for the intestinal microbes, thereby functioning as a host-specific determinant affecting the microbiota composition and human health. Polysaccharides 6-13 LOC100508689 Homo sapiens 0-5 25106027-4 2014 Reflecting the structural diversity of mucin glycans and their prime location, commensal and pathogenic microbes have evolved a range of adhesins allowing their interaction with the host. Polysaccharides 45-52 LOC100508689 Homo sapiens 39-44 24307362-3 2013 To enable future binding studies of mucin glycan and glycopeptide probes, a method that gives flexible and efficient access to all common mucin core-glycosylated amino acids was developed. Polysaccharides 42-48 LOC100508689 Homo sapiens 36-41 22180206-1 2011 Site-specific characterisation of mucin-type O-linked glycosylation is an analytical challenge due to glycan heterogeneity, lack of glycosylation site consensus sequence and high density of occupied glycosylation sites. Polysaccharides 102-108 LOC100508689 Homo sapiens 34-39 24204617-0 2013 Utilisation of mucin glycans by the human gut symbiont Ruminococcus gnavus is strain-dependent. Polysaccharides 21-28 LOC100508689 Homo sapiens 15-20 24204617-5 2013 Comparative genomic analysis of the two R. gnavus strains highlighted potential clusters and glycoside hydrolases (GHs) responsible for the breakdown and utilization of mucin-derived glycans. Polysaccharides 183-190 LOC100508689 Homo sapiens 169-174 23980712-5 2013 Moreover, our data indicate that the cell-repellent effect is dependent on mucin-associated glycans because their removal results in a loss of effective cell-repulsion. Polysaccharides 92-99 LOC100508689 Homo sapiens 75-80 23359164-3 2013 Given the complexity of mucin glycans, several sophisticated analytical tools such as HPLC, mass spectrometry, and lectin sandwich assays are employed for glyco-analysis of mucins. Polysaccharides 30-37 LOC100508689 Homo sapiens 24-29 23359164-5 2013 We described in this chapter the utility of the simple electrophoresis/immunoblotting method to examine the mucin glycan epitopes, using specific antibodies and lectins. Polysaccharides 114-120 LOC100508689 Homo sapiens 108-113 22444368-1 2012 Mucin glycoproteins present a complex structural landscape arising from the multiplicity of glycosylation patterns afforded by their numerous serine and threonine glycosylation sites, often in clusters, and with variations in respective glycans. Polysaccharides 237-244 LOC100508689 Homo sapiens 0-5 22183981-2 2012 Mucin-type O-glycosylation, consisting of glycans attached via O-linked N-acetylgalactosamine (GalNAc) to serine and threonine residues, is one of the most abundant forms of protein glycosylation in animals. Polysaccharides 42-49 LOC100508689 Homo sapiens 0-5 22183981-3 2012 Although most protein glycosylation is controlled by one or two genes encoding the enzymes responsible for the initiation of glycosylation, i.e. the step where the first glycan is attached to the relevant amino acid residue in the protein, mucin-type O-glycosylation is controlled by a large family of up to 20 homologous genes encoding UDP-GalNAc:polypeptide GalNAc-transferases (GalNAc-Ts) (EC 2.4.1.41). Polysaccharides 170-176 LOC100508689 Homo sapiens 240-245 22329400-4 2012 Results show that (i) the radiolabeled mucin glycoproteins of each of the cancer cell lines studied (T47D, MCF7, LS180, LNCaP, SKOV3, HL60, DU4475, and HepG2) is distinct either in terms of the specific glycans presented or their relative distribution. Polysaccharides 203-210 LOC100508689 Homo sapiens 39-44 22259131-1 2012 The characterization of mucin-type O-glycosylation is fraught with extreme difficulty at almost every level of analysis: from difficulties in obtaining glycopeptides suitable for study, their structural heterogeneity, lack of broad acting glycosidase tools capable of simplifying the glycans, and finally the vast complexity of performing analysis on multiply glycosylated glycopeptides. Polysaccharides 284-291 LOC100508689 Homo sapiens 24-29 23782552-7 2013 Moreover, antibiotics altered the active fraction of enzymes controlling the thickness, composition and consistency of the mucin glycans. Polysaccharides 129-136 LOC100508689 Homo sapiens 123-128 23763610-2 2013 Here, we describe the design and construction of peptide-free multivalent glycosylated nanoscale constructs as potential synthetic cancer vaccines that generate significant titers of antibodies selective for aberrant mucin glycans. Polysaccharides 223-230 LOC100508689 Homo sapiens 217-222 22950532-7 2012 Furthermore, we demonstrate the compatibility of this novel staining procedure with glycan analysis using porcine gastric mucin as a model mucin. Polysaccharides 84-90 LOC100508689 Homo sapiens 139-144 21514575-1 2011 For the investigation of glycosidases, and for the construction of glycan arrays the p-nitrophenyl- and p-aminophenyl glycosides of mucin O-glycan core structures 1-7 and the 2,6-ST-antigen have been chemically synthesized using d-galactose as a precursor for GalNAc residues. Polysaccharides 67-73 LOC100508689 Homo sapiens 132-137 21507958-3 2011 Bacteria colonizing the mucosal layer that overlies the gut epithelium are exposed to highly sulfated glycans (i.e. mucin and glycosaminoglycans). Polysaccharides 102-109 LOC100508689 Homo sapiens 116-121 21669976-2 2011 Here, we show the KCNE1 regulatory subunit is O-glycosylated with mucin-type glycans in vivo. Polysaccharides 77-84 LOC100508689 Homo sapiens 66-71 20395854-7 2010 RESULTS: The detection of a glycan variant on MUC5AC using the lectin wheat-germ agglutinin discriminated mucin-producing cystic tumors (mucinous cystic neoplasms+intraductal papillary mucinous neoplasms) from benign cystic lesions (serous cystadenomas+pseudocysts) with a 78% sensitivity at 80% specificity, and when used in combination with cyst fluid CA 19-9 gave a sensitivity of 87% at 86% specificity. Polysaccharides 28-34 LOC100508689 Homo sapiens 106-111 20615996-9 2011 This observation that normal human individuals carry a uniform MUC2 mucin glycan array in colon may indicate such a specific selection. Polysaccharides 74-80 LOC100508689 Homo sapiens 68-73 20974960-3 2010 Analysis of the 2,214,650-bp genome of Bifidobacterium bifidum PRL2010, a strain isolated from infant stool, revealed a nutrient-acquisition strategy that targets host-derived glycans, such as those present in mucin. Polysaccharides 176-183 LOC100508689 Homo sapiens 210-215 20863315-0 2010 Ionic liquids in oligosaccharide synthesis: towards mucin-type glycan probes. Polysaccharides 63-69 LOC100508689 Homo sapiens 52-57 20144722-1 2010 Newly emerging genetic studies have revealed that a subset of the family of glycosyltransferases responsible for the formation of mucin-type O glycans is essential for normal development. Polysaccharides 143-150 LOC100508689 Homo sapiens 130-135 19191477-8 2009 The structure of BT1043 complexed with N-acetyllactosamine reveals that recognition is mediated via hydrogen bonding interactions with the reducing end of beta-N-acetylglucosamine, suggesting a role in binding glycans liberated from the mucin polypeptide. Polysaccharides 210-217 LOC100508689 Homo sapiens 237-242 20306342-5 2010 Interestingly, the lectin showed high affinity for glycans which are part of ovarian cancer marker CA125, a high molecular weight mucin containing high mannose and complex bisecting type N-linked glycans as well core 1 and 2 type O-glycans. Polysaccharides 51-58 LOC100508689 Homo sapiens 130-135 19368346-9 2009 This work revealed the presence of three types of mucin with distinct glycan profiles in human pancreatic juice. Polysaccharides 70-76 LOC100508689 Homo sapiens 50-55 19191477-9 2009 This is in contrast to CBM 32 family members that target the terminal nonreducing galactose residue of mucin glycans. Polysaccharides 109-116 LOC100508689 Homo sapiens 103-108 12297228-2 2002 In an ex vivo system based on porcine colonic tissue various neutral and acidic polysaccharides were tested concerning their bioadhesive potential in order to form artificial mucin layers on colon epithelial membranes. Polysaccharides 80-95 LOC100508689 Homo sapiens 175-180 17303715-1 2007 Mucin glycan is the primary determinant of mucin functions. Polysaccharides 6-12 LOC100508689 Homo sapiens 0-5 17303715-1 2007 Mucin glycan is the primary determinant of mucin functions. Polysaccharides 6-12 LOC100508689 Homo sapiens 43-48 14749330-10 2004 The results show that the mucin polypeptide undergoes dimerization and then becomes substituted with GalNAc residues prior to glycan elaboration to produce a mature mucin dimer, which then undergoes multimerization. Polysaccharides 126-132 LOC100508689 Homo sapiens 26-31 12652802-4 2002 On the other hand, gland mucous cell-type mucin secreted from the normal gastric mucosa characteristically contains GlcNAc alpha 1-->4Gal beta-->R structure, and the alpha 4GnT is critical for the biosynthesis of this unique glycan. Polysaccharides 231-237 LOC100508689 Homo sapiens 42-47 12952970-1 2003 Mucin glycans were isolated from different regions of the normal human intestine (ileum, cecum, transverse and sigmoid colon, and rectum) of two individuals with ALeb blood group. Polysaccharides 6-13 LOC100508689 Homo sapiens 0-5 11577689-0 2001 Sd(a)-antigen-like structures carried on core 3 are prominent features of glycans from the mucin of normal human descending colon. Polysaccharides 74-81 LOC100508689 Homo sapiens 91-96 11577689-1 2001 This paper describes structural characterization by NMR, MS and degradative studies of mucin glycans from normal human descending colon obtained freshly at autopsy. Polysaccharides 93-100 LOC100508689 Homo sapiens 87-92 14533804-0 2001 Biosynthesis and function of beta 1,6 branched mucin-type glycans. Polysaccharides 58-65 LOC100508689 Homo sapiens 47-52 14533804-3 2001 For instance, synthesis of the core 2 beta1,6 branched structure in the mucin glycan chain by C2GnT enables the expression of functional structures at the termini of polylactosamine chains, such as blood group antigens and sialyl Lewis x. Polysaccharides 78-84 LOC100508689 Homo sapiens 72-77 14533804-5 2001 The family of enzymes which creates beta1,6 branched structure in mucin glycans is proving to be quite complex, since multiple isoforms appear to exist for these enzymes, and some of the enzymes are adept at forming more than one type of beta1,6 branched structure, as in the case of C2GnT-M. Polysaccharides 72-79 LOC100508689 Homo sapiens 66-71 11425189-7 2000 We conclude that this novel chemical deglycosylation method that causes selective cleavage of distinct glycans will be useful in unmasking various mucin gene products and glycoproteins containing similar O-glycosidic linkages in the tissue sections of formalin-fixed paraffin embedded normal and pathological tissues. Polysaccharides 103-110 LOC100508689 Homo sapiens 147-152 10584881-7 1999 The O-linked glycans (three mucin O-GalNAc type glycans with variable degrees of sialylation, one O-HexNAc monosaccharide glycan) have not previously been reported. Polysaccharides 13-20 LOC100508689 Homo sapiens 28-33 10584881-8 1999 The finding of mucin O-GalNAc type glycans was supported by the prediction of potential O-GalNAc glycosylation sites on the protein backbone by analysis of the AFP structure by molecular modelling. Polysaccharides 35-42 LOC100508689 Homo sapiens 15-20 9756896-11 1998 The data further suggest that hydroxyamino acid spacing may contribute to the regulation of glycan length, thereby, providing a mechanism for maintaining an optimally expanded, protease resistant, mucin conformation. Polysaccharides 92-98 LOC100508689 Homo sapiens 197-202 8962658-10 1996 Using semi-quantitative immunohistochemical methods we have shown that in women suffering recurrent spontaneous miscarriage, mid secretory phase levels of MUC1 core protein and mucin-associated glycans are reduced (Serle et al., Fertil. Polysaccharides 194-201 LOC100508689 Homo sapiens 177-182 14707484-5 2002 Many of the glycans detected on the MUC1 mucin were common to both cell types, as would be predicted from biosynthetic constraints. Polysaccharides 12-19 LOC100508689 Homo sapiens 41-46 11425189-0 2000 Alkali-catalyzed beta-elimination of periodate-oxidized glycans: a novel method of chemical deglycosylation of mucin gene products in paraffin embedded sections. Polysaccharides 56-63 LOC100508689 Homo sapiens 111-116 10070964-0 1999 Human colon adenocarcinomas express a MUC1-associated novel carbohydrate epitope on core mucin glycans defined by a monoclonal antibody (A10) raised against murine Ehrlich tumor cells. Polysaccharides 95-102 LOC100508689 Homo sapiens 89-94 10070964-9 1999 Deglycosylation studies with trifluoromethanesulfonic acid pointed to the involvement of core mucin glycans in the A10 epitope. Polysaccharides 100-107 LOC100508689 Homo sapiens 94-99 10070964-12 1999 Taken together, the present results point to A10 defining a core 6-related epitope on core mucin glycans expressed by colon cancer MUC1 not previously associated with human cancer. Polysaccharides 97-104 LOC100508689 Homo sapiens 91-96 9405387-0 1997 Sulfated lewis X determinants as a major structural motif in glycans from LS174T-HM7 human colon carcinoma mucin. Polysaccharides 61-68 LOC100508689 Homo sapiens 107-112 8928251-3 1996 Mucin is composed of a central peptide core with polysaccharide chains arranged radially from the core ("bottle brushappearance"). Polysaccharides 49-63 LOC100508689 Homo sapiens 0-5 8595262-4 1995 The minimal structure recognized by MeAI on the porcine mucin glycans is the O-glycan core Gal beta 1,3GalNAc-ol, whereas MeAII has a more extended site and interacts with a biantennary O-glycan possessing the terminal trisaccharide Fuc alpha 1,2 (GalNAc alpha 1,3) Gal beta 1,4. Polysaccharides 62-69 LOC100508689 Homo sapiens 56-61