PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 32343311-6 2020 When the U2AF heterodimer is bound to a strong, uridine-rich splice site, U2AF2 switches to a lower FRET value characteristic of an open, side-by-side arrangement of the RRMs. Uridine 48-55 U2 small nuclear RNA auxiliary factor 2 Homo sapiens 74-79 16818232-2 2006 Since Py tracts are relatively poorly conserved in higher eukaryotes, U2AF(65) is faced with the problem of specifying uridine-rich sequences, yet tolerating a variety of nucleotide substitutions found in natural Py tracts. Uridine 119-126 U2 small nuclear RNA auxiliary factor 2 Homo sapiens 70-77 21074620-7 2011 The complementary aspects of combining NMR and SAS data for studying multi-domain proteins, i.e. where weakly interacting domains are connected by flexible linkers, are illustrated with the structural analysis of the tandem RNA recognition motif (RRM) domains (RRM1-RRM2) of the human splicing factor U2AF65 bound to a nine-uridine (U9) RNA oligonucleotide. Uridine 324-331 U2 small nuclear RNA auxiliary factor 2 Homo sapiens 301-307 18549497-4 2008 The PY tract is a uridine-rich region at the 3" end of introns that acts as a binding site for U2AF65, a key factor in splicing machinery recruitment. Uridine 18-25 U2 small nuclear RNA auxiliary factor 2 Homo sapiens 95-101 16818232-3 2006 To better understand these apparently contradictory RNA binding characteristics, the X-ray structure of the U2AF(65) RNA binding domain bound to a Py tract composed of seven uridines has been determined at 2.5 A resolution. Uridine 174-182 U2 small nuclear RNA auxiliary factor 2 Homo sapiens 108-116 7761834-3 1995 The uridine-rich degenerate sequences selected by U2AF65 are similar to those present in the diverse array of natural metazoan Py-tracts. Uridine 4-11 U2 small nuclear RNA auxiliary factor 2 Homo sapiens 50-56 12558503-2 2003 We report that U2AF(65) can also promote the recruitment of U1 snRNP to weak 5" splice sites that are followed by uridine-rich sequences. Uridine 114-121 U2 small nuclear RNA auxiliary factor 2 Homo sapiens 15-22 35524551-8 2022 Moreover, enhanced UV-crosslinking and immunoprecipitation of endogenous U2AF2 in human erythroleukemia cells showed uridine-sensitive binding sites, with lower sequence conservation at the central nucleotide positions of otherwise uridine-rich, U2AF2-bound splice sites. Uridine 117-124 U2 small nuclear RNA auxiliary factor 2 Homo sapiens 73-78 35524551-8 2022 Moreover, enhanced UV-crosslinking and immunoprecipitation of endogenous U2AF2 in human erythroleukemia cells showed uridine-sensitive binding sites, with lower sequence conservation at the central nucleotide positions of otherwise uridine-rich, U2AF2-bound splice sites. Uridine 117-124 U2 small nuclear RNA auxiliary factor 2 Homo sapiens 246-251 35524551-8 2022 Moreover, enhanced UV-crosslinking and immunoprecipitation of endogenous U2AF2 in human erythroleukemia cells showed uridine-sensitive binding sites, with lower sequence conservation at the central nucleotide positions of otherwise uridine-rich, U2AF2-bound splice sites. Uridine 232-239 U2 small nuclear RNA auxiliary factor 2 Homo sapiens 73-78 35524551-8 2022 Moreover, enhanced UV-crosslinking and immunoprecipitation of endogenous U2AF2 in human erythroleukemia cells showed uridine-sensitive binding sites, with lower sequence conservation at the central nucleotide positions of otherwise uridine-rich, U2AF2-bound splice sites. Uridine 232-239 U2 small nuclear RNA auxiliary factor 2 Homo sapiens 246-251