PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 19022160-0 2008 Calcium-dependent NOX5 nicotinamide adenine dinucleotide phosphate oxidase contributes to vascular oxidative stress in human coronary artery disease. Calcium 0-7 NADPH oxidase 5 Homo sapiens 18-22 19022160-3 2008 Several NOX homologues have been identified, but their relative contribution to vascular ROS production in coronary artery disease (CAD) is unclear; NOX5 is a unique homolog in that it is calcium dependent and thus could be activated by vasoconstrictor hormones. Calcium 188-195 NADPH oxidase 5 Homo sapiens 149-153 19022160-8 2008 Calcium-dependent NADPH-driven production of ROS in vascular membranes, reflecting NOX5 activity, was increased 7-fold in CAD and correlated significantly with NOX5 mRNA levels among subjects. Calcium 0-7 NADPH oxidase 5 Homo sapiens 83-87 19022160-8 2008 Calcium-dependent NADPH-driven production of ROS in vascular membranes, reflecting NOX5 activity, was increased 7-fold in CAD and correlated significantly with NOX5 mRNA levels among subjects. Calcium 0-7 NADPH oxidase 5 Homo sapiens 160-164 19022160-10 2008 CONCLUSIONS: These studies identify NOX5 as a novel, calcium-dependent source of ROS in atherosclerosis. Calcium 53-60 NADPH oxidase 5 Homo sapiens 36-40 17275676-3 2007 Whereas NOX1, NOX2, and NOX4 are expressed in endothelial cells, a functional role of NOX5 containing additional N-terminal calcium-binding domains of varying sequences has not been reported in these cells. Calcium 124-131 NADPH oxidase 5 Homo sapiens 86-90 18160052-3 2008 Confocal studies demonstrated that the initial calcium influx generated by H(2)O(2) is amplified by a feedback mechanism involving NOX5-dependent superoxide production and H(2)O(2). Calcium 47-54 NADPH oxidase 5 Homo sapiens 131-135 17164239-2 2007 In contrast to other Nox isoforms, the activity of Nox5 does not require the presence of accessory proteins and is entirely dependent on the elevation of intracellular calcium. Calcium 168-175 NADPH oxidase 5 Homo sapiens 51-55 17164239-3 2007 Previous studies have shown that the EC(50) of Nox5 for calcium is relatively high and raises the question of whether Nox5 can be sufficiently activated in cells that do not experience extreme elevations of intracellular calcium. Calcium 56-63 NADPH oxidase 5 Homo sapiens 47-51 17164239-7 2007 The ability of PMA to increase Nox5 activity was abolished by calcium chelation and was a direct effect on enzyme activity, since PMA increased the calcium sensitivity of Nox5 in a cell-free assay. Calcium 62-69 NADPH oxidase 5 Homo sapiens 31-35 17164239-7 2007 The ability of PMA to increase Nox5 activity was abolished by calcium chelation and was a direct effect on enzyme activity, since PMA increased the calcium sensitivity of Nox5 in a cell-free assay. Calcium 62-69 NADPH oxidase 5 Homo sapiens 171-175 17164239-7 2007 The ability of PMA to increase Nox5 activity was abolished by calcium chelation and was a direct effect on enzyme activity, since PMA increased the calcium sensitivity of Nox5 in a cell-free assay. Calcium 148-155 NADPH oxidase 5 Homo sapiens 31-35 17164239-7 2007 The ability of PMA to increase Nox5 activity was abolished by calcium chelation and was a direct effect on enzyme activity, since PMA increased the calcium sensitivity of Nox5 in a cell-free assay. Calcium 148-155 NADPH oxidase 5 Homo sapiens 171-175 17164239-12 2007 In summary, the phosphorylation of Nox5 at key residues facilitates enzyme activation at lower levels of intracellular calcium and may provide an avenue for enzyme activation in response to a greater variety of extracellular stimuli. Calcium 119-126 NADPH oxidase 5 Homo sapiens 35-39 17275676-10 2007 These data show that endothelial cells express different NOX5 variants including NOX5S lacking calcium-binding domains. Calcium 95-102 NADPH oxidase 5 Homo sapiens 57-61 34269800-2 2021 This study tested the hypothesis that lysophosphatidylcholine (LPC), a proatherogenic component of oxLDL, induces endothelial calcium influx, which drives NOX5-dependent reactive oxygen species (ROS) production, oxidative stress, and endothelial cell dysfunction. Calcium 126-133 NADPH oxidase 5 Homo sapiens 155-159 12686516-7 2003 The calcium ionophore ionomycin dramatically stimulated ferricytochrome c reduction in cell media, further supporting the importance of NOX5 for ROS production. Calcium 4-11 NADPH oxidase 5 Homo sapiens 136-140 34139309-3 2021 We studied the effect of NO and heme-NO on the transmembrane signaling enzyme NADPH oxidase 5 (NOX5), a heme protein which produces superoxide in response to increases in intracellular calcium. Calcium 185-192 NADPH oxidase 5 Homo sapiens 78-93 34139309-3 2021 We studied the effect of NO and heme-NO on the transmembrane signaling enzyme NADPH oxidase 5 (NOX5), a heme protein which produces superoxide in response to increases in intracellular calcium. Calcium 185-192 NADPH oxidase 5 Homo sapiens 95-99 34269800-8 2021 NOX1/4 dual inhibition and selective NOX1 inhibition only decreased ROS generation at 4 h. LPC increased HAEC intracellular calcium, important for NOX5 activation, and this was blocked by nifedipine and thapsigargin. Calcium 125-132 NADPH oxidase 5 Homo sapiens 148-152 34269800-11 2021 CONCLUSION: These results suggest that NOX5, by mechanisms linked to increased intracellular calcium, is key to early LPC-induced endothelial oxidative stress and pro-inflammatory processes. Calcium 93-100 NADPH oxidase 5 Homo sapiens 39-43 32738790-1 2020 NADPH oxidase 5 (NOX5) is a transmembrane signaling enzyme that produces superoxide in response to elevated cytosolic calcium. Calcium 118-125 NADPH oxidase 5 Homo sapiens 0-15 32564697-4 2020 We examined how VSMC phenotypic switching influences vascular calcification and the possible role of the uniquely calcium-dependent ROS-forming NADPH oxidase 5 (Nox5). Calcium 114-121 NADPH oxidase 5 Homo sapiens 144-159 32564697-4 2020 We examined how VSMC phenotypic switching influences vascular calcification and the possible role of the uniquely calcium-dependent ROS-forming NADPH oxidase 5 (Nox5). Calcium 114-121 NADPH oxidase 5 Homo sapiens 161-165 32738790-1 2020 NADPH oxidase 5 (NOX5) is a transmembrane signaling enzyme that produces superoxide in response to elevated cytosolic calcium. Calcium 118-125 NADPH oxidase 5 Homo sapiens 17-21 32155782-7 2020 Protein kinase C (PKC) activation and intracellular calcium level (Ca++) mobilization increased ROS production and NOX5 overexpression, which promoted a COX-2/PGE2 response in vitro. Calcium 52-59 NADPH oxidase 5 Homo sapiens 115-119 31785178-0 2020 On the mechanism of calcium-dependent activation of NADPH oxidase 5 (NOX5). Calcium 20-27 NADPH oxidase 5 Homo sapiens 52-67 31785178-0 2020 On the mechanism of calcium-dependent activation of NADPH oxidase 5 (NOX5). Calcium 20-27 NADPH oxidase 5 Homo sapiens 69-73 31785178-4 2020 We show that the calmodulin-like regulatory EF-domain of NOX5 is partially unfolded and detached from the rest of the protein in the absence of calcium. Calcium 144-151 NADPH oxidase 5 Homo sapiens 57-61 31785178-7 2020 Altogether, our work shows that calcium induces an unfolded-to-folded transition of the EF-domain that promotes direct interaction with a conserved regulatory region, resulting in NOX5 activation. Calcium 32-39 NADPH oxidase 5 Homo sapiens 180-184 31203730-0 2020 Metal binding and conformational studies of the calcium binding domain of NADPH oxidase 5 reveal its similarity and difference to calmodulin. Calcium 48-55 NADPH oxidase 5 Homo sapiens 74-89 31203730-1 2020 The superoxide-generating activity of Nox5 is regulated by Ca2+ flux, primarily through its self-contained calcium binding domain (EFD). Calcium 107-114 NADPH oxidase 5 Homo sapiens 38-42 30936599-0 2019 Inhibition of CatSper and Hv1 Channels and NOX5 Enzyme Affect Progesterone-Induced Increase of Intracellular Calcium Concentration and ROS Generation in Human Sperm. Calcium 109-116 NADPH oxidase 5 Homo sapiens 43-47 30936599-2 2019 Calcium activates NADPH oxidase-5 (NOX5) that leads to ROS generation. Calcium 0-7 NADPH oxidase 5 Homo sapiens 18-33 30936599-2 2019 Calcium activates NADPH oxidase-5 (NOX5) that leads to ROS generation. Calcium 0-7 NADPH oxidase 5 Homo sapiens 35-39 27391469-3 2016 Contrary to other members of NADPH-oxidases family, NOX5 has its own regulatory calcium-binding domain and thus could be activated directly by calcium ions. Calcium 80-87 NADPH oxidase 5 Homo sapiens 52-56 29907654-16 2018 Our findings define a novel function for vascular Nox5, linking calcium and reactive oxygen species to the pro-contractile molecular machinery in vascular smooth muscle cells. Calcium 64-71 NADPH oxidase 5 Homo sapiens 50-54 29907654-1 2018 BACKGROUND: NADPH Oxidase 5 (Nox5) is a calcium-sensitive superoxide-generating Nox. Calcium 40-47 NADPH oxidase 5 Homo sapiens 29-33 27614387-3 2016 Nox1-3 are dependent on different cytosolic subunits, Nox4 seems to be constitutively active and Nox5 is directly activated by calcium. Calcium 127-134 NADPH oxidase 5 Homo sapiens 97-101 27391469-3 2016 Contrary to other members of NADPH-oxidases family, NOX5 has its own regulatory calcium-binding domain and thus could be activated directly by calcium ions. Calcium 143-150 NADPH oxidase 5 Homo sapiens 52-56 27391469-6 2016 Our data strongly suggest that oxidation of calcium-binding domain of NOX5 could be implicated in its inactivation, serving as a possible defense mechanism against oxidative stress. Calcium 44-51 NADPH oxidase 5 Homo sapiens 70-74 24992705-1 2014 Nox5 is an EF-hand containing, calcium-dependent isoform of the NADPH oxidase family of reactive oxygen species (ROS) generating enzymes. Calcium 31-38 NADPH oxidase 5 Homo sapiens 0-4 26901778-8 2016 Moreover, the acid-induced increase in Rho kinase activity and in NOX5-S mRNA expression was blocked by the removal of calcium in both FLO-1 and OE33 cells. Calcium 119-126 NADPH oxidase 5 Homo sapiens 66-70 26901778-9 2016 The calcium ionophore A23187 significantly increased the Rho kinase activity and NOX5-S mRNA expression. Calcium 4-11 NADPH oxidase 5 Homo sapiens 81-85 26901778-12 2016 It is possible that persistent acid reflux present in BE patients may increase the intracellular calcium, activate ROCK2 and thereby upregulate NOX5-S. High levels of reactive oxygen species derived from NOX5-S may cause DNA damage and thereby contribute to the progression from BE to EA. Calcium 97-104 NADPH oxidase 5 Homo sapiens 204-208 25797883-10 2015 Transfection of NOX5-specific siRNA and chemical inhibition of NOX5 abrogated calcium-induced superoxide production and increased caspase 3-mediated apoptosis in Karpas-299 cells. Calcium 78-85 NADPH oxidase 5 Homo sapiens 16-20 25797883-10 2015 Transfection of NOX5-specific siRNA and chemical inhibition of NOX5 abrogated calcium-induced superoxide production and increased caspase 3-mediated apoptosis in Karpas-299 cells. Calcium 78-85 NADPH oxidase 5 Homo sapiens 63-67 26456056-7 2015 In cell lysates using a co-IP approach, Hsp90 binds to Nox5 but not Nox4, and the degree of binding can be influenced by calcium-dependent stimuli. Calcium 121-128 NADPH oxidase 5 Homo sapiens 55-59 24992705-6 2014 Focusing on M77K, Nox5 activity was dramatically reduced in unstimulated cells and following challenge with both calcium- and phosphorylation-dependent stimuli despite equivalent levels of expression. Calcium 113-120 NADPH oxidase 5 Homo sapiens 18-22 23439561-8 2013 Knockdown of NADPH oxidase, NOX5-S, a variant lacking calcium-binding domains, by NOX5 siRNA significantly inhibited acid-induced increase in mPGES1 expression, thymidine incorporation, and PGE2 production. Calcium 54-61 NADPH oxidase 5 Homo sapiens 28-32 24180474-10 2014 Calcium-dependent Nox5 has been implicated in oxidative damage in human atherosclerosis. Calcium 0-7 NADPH oxidase 5 Homo sapiens 18-22 24505490-2 2014 The activity of Nox5 and production of reactive oxygen species is regulated by intracellular calcium levels and phosphorylation. Calcium 93-100 NADPH oxidase 5 Homo sapiens 16-20 23439561-8 2013 Knockdown of NADPH oxidase, NOX5-S, a variant lacking calcium-binding domains, by NOX5 siRNA significantly inhibited acid-induced increase in mPGES1 expression, thymidine incorporation, and PGE2 production. Calcium 54-61 NADPH oxidase 5 Homo sapiens 82-86 22503554-2 2012 NOX1, NOX2, and NOX4 are important sources of reactive oxygen species in the heart, but knowledge of the calcium-dependent NOX5 in the heart is lacking. Calcium 105-112 NADPH oxidase 5 Homo sapiens 123-127 21319793-1 2011 Nox5 belongs to the calcium-regulated subfamily of NADPH oxidases (Nox). Calcium 20-27 NADPH oxidase 5 Homo sapiens 0-4 22387196-2 2012 Nox5 was the last of the conventional Nox isoforms to be identified and is a calcium-dependent enzyme that does not depend on accessory subunits for activation. Calcium 77-84 NADPH oxidase 5 Homo sapiens 0-4 22490336-2 2012 The calcium binding domain (CaBD) of NOX5 contains four EF-hands, each binding one calcium ion. Calcium 4-11 NADPH oxidase 5 Homo sapiens 37-41 22490336-2 2012 The calcium binding domain (CaBD) of NOX5 contains four EF-hands, each binding one calcium ion. Calcium 83-90 NADPH oxidase 5 Homo sapiens 37-41 21642394-2 2011 Nox5 is a novel Nox isoform whose activity is regulated by intracellular calcium levels. Calcium 73-80 NADPH oxidase 5 Homo sapiens 0-4 21642394-3 2011 We have reported that the activity and calcium-sensitivity of Nox5 can also be modulated by direct phosphorylation. Calcium 39-46 NADPH oxidase 5 Homo sapiens 62-66 21642394-7 2011 The ability of CAMKII to increase Nox5 activity was also observed with fixed calcium concentrations in an isolated enzyme activity assay. Calcium 77-84 NADPH oxidase 5 Homo sapiens 34-38 21297032-2 2011 Instead, the primary driving force for Nox5 activity is calcium. Calcium 56-63 NADPH oxidase 5 Homo sapiens 39-43 21297032-4 2011 This apparent calcium-independent activation was found to be mediated by the PKC-dependent phosphorylation of specific serine and threonine residues on Nox5 which increased the calcium sensitivity of the enzyme and enabled activation at resting levels of calcium. Calcium 14-21 NADPH oxidase 5 Homo sapiens 152-156 21297032-4 2011 This apparent calcium-independent activation was found to be mediated by the PKC-dependent phosphorylation of specific serine and threonine residues on Nox5 which increased the calcium sensitivity of the enzyme and enabled activation at resting levels of calcium. Calcium 177-184 NADPH oxidase 5 Homo sapiens 152-156 21297032-4 2011 This apparent calcium-independent activation was found to be mediated by the PKC-dependent phosphorylation of specific serine and threonine residues on Nox5 which increased the calcium sensitivity of the enzyme and enabled activation at resting levels of calcium. Calcium 177-184 NADPH oxidase 5 Homo sapiens 152-156 21319793-2 2011 Like other calcium-regulated Noxes, Nox5 has an EF-hand-containing calcium-binding domain at its N-terminus, a transmembrane heme-containing region, and a C-terminal dehydrogenase (DH) domain that binds FAD and NADPH. Calcium 11-18 NADPH oxidase 5 Homo sapiens 36-40 21319793-2 2011 Like other calcium-regulated Noxes, Nox5 has an EF-hand-containing calcium-binding domain at its N-terminus, a transmembrane heme-containing region, and a C-terminal dehydrogenase (DH) domain that binds FAD and NADPH. Calcium 67-74 NADPH oxidase 5 Homo sapiens 36-40 21319793-5 2011 Oligomerization of full-length Nox5 was demonstrated using co-immunoprecipitation of coexpressed, differentially tagged forms of Nox5 and occurred in a manner independent of calcium ion. Calcium 174-181 NADPH oxidase 5 Homo sapiens 31-35 21319793-6 2011 Several approaches were used to show that the DH domain mediates oligomerization: Nox5 could be isolated as a multimer when the calcium-binding domain and/or the N-terminal polybasic region (PBR-N) was deleted, but deletion of the DH domain eliminated oligomerization. Calcium 128-135 NADPH oxidase 5 Homo sapiens 82-86 21319793-10 2011 As a result of oligomerization, the short, calcium-independent splice form, Nox5S, may function as an endogenous inhibitor of calcium-stimulated ROS generation by full-length Nox5. Calcium 43-50 NADPH oxidase 5 Homo sapiens 76-80 21319793-10 2011 As a result of oligomerization, the short, calcium-independent splice form, Nox5S, may function as an endogenous inhibitor of calcium-stimulated ROS generation by full-length Nox5. Calcium 126-133 NADPH oxidase 5 Homo sapiens 76-80 20028137-0 2010 Identification of the binding site for the regulatory calcium-binding domain in the catalytic domain of NOX5. Calcium 54-61 NADPH oxidase 5 Homo sapiens 104-108 20648216-0 2010 Conformational States and kinetics of the calcium binding domain of NADPH oxidase 5. Calcium 42-49 NADPH oxidase 5 Homo sapiens 68-83