PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 15572240-0 2004 Review of some unusual effects of calcium binding to fibrinogen. Calcium 34-41 fibrinogen beta chain Homo sapiens 53-63 16643450-3 2006 Low calcium ionophore (A23187) concentrations induced Fg binding but not annexin V binding. Calcium 4-11 fibrinogen beta chain Homo sapiens 54-56 16358236-7 2005 On multivariate Cox"s regression analyses, including traditional risk factors and history of previous CV events, Fib (hazard ratio (HR) associated with 100 mg/dL increase in plasma Fib 1.29 95% confidence interval (CI) 1.03-1.63 (p=0.03)) and calcium x phosphate product (HR associated with a 5 mg2/dL2 increase 1.25 95% CI 1.05-1.49 (p=0.01)) emerged as independent CV event predictors. Calcium 243-250 fibrinogen beta chain Homo sapiens 113-116 15837518-3 2005 Both strongly and weakly bound calcium ions are important for maintenance of fibrinogen"s structure and functions. Calcium 31-38 fibrinogen beta chain Homo sapiens 77-87 15572240-1 2004 Calcium binding curves of human and bovine fibrinogen were obtained by using a calcium sensitive electrode. Calcium 0-7 fibrinogen beta chain Homo sapiens 43-53 15572240-1 2004 Calcium binding curves of human and bovine fibrinogen were obtained by using a calcium sensitive electrode. Calcium 79-86 fibrinogen beta chain Homo sapiens 43-53 15572240-7 2004 In the absence of Ca2+ clotting elicited a 40% increase in the enthalpy of thermal denaturation of the D domain of fibrinogen, but the position of the peak increased only by 0.4 degrees C. However, with clotting in the presence of 10(-3) M calcium the former increased by 70-75% and the latter by 11.0 degrees C, while these parameters of the E-domain remained unchanged. Calcium 240-247 fibrinogen beta chain Homo sapiens 115-125 15572240-9 2004 These had to be corrected, because the drop in free calcium was partly compensated by release of some calcium that was already bound to fibrinogen. Calcium 102-109 fibrinogen beta chain Homo sapiens 136-146 11707691-11 2001 Taken together, these observations suggest that the antiplatelet activity of GTC is be mediated by inhibition of cytoplasmic calcium increase, which leads to the inhibition of fibrinogen-GPIIb/IIIa binding via the activation of Ca(2+)-ATPase and inhibition of IP(3) formation. Calcium 125-132 fibrinogen beta chain Homo sapiens 176-186 14708628-7 2003 Surprisingly, fibrin and fibrinogen caused significant detachment of keratinocytes which was prevented by the addition of calcium. Calcium 122-129 fibrinogen beta chain Homo sapiens 25-35 12842247-0 2003 Relation of C-reactive protein and fibrinogen to coronary artery calcium in subjects with systemic hypertension. Calcium 65-72 fibrinogen beta chain Homo sapiens 35-45 12356313-0 2002 2.8 A crystal structures of recombinant fibrinogen fragment D with and without two peptide ligands: GHRP binding to the "b" site disrupts its nearby calcium-binding site. Calcium 149-156 fibrinogen beta chain Homo sapiens 40-50 12356313-5 2002 Of major interest in our structures is that a previously identified calcium site in plasma fibrinogen is absent when Gly-His-Arg-Pro-amide is bound. Calcium 68-75 fibrinogen beta chain Homo sapiens 91-101 11955079-3 2002 We have examined variant fibrinogens with alanine at position gamma318 and/or gamma320 and found that calcium binding, fibrin polymerization, and fibrinogen-mediated platelet aggregation, but not FXIIIa-catalyzed cross-linking, were abnormal. Calcium 102-109 fibrinogen beta chain Homo sapiens 25-35 12871330-8 2003 Immunoprecipitation of the digests with antifibrinogen antibody showed 70 +/- 8% of fibrinogen-bound FGF-2 in the presence of calcium but only 15 +/- 4% in its absence, indicating that cleavage of D1 to D2 and D3 is critical in binding. Calcium 126-133 fibrinogen beta chain Homo sapiens 44-54 11435303-3 2001 Polymerization of purified fibrinogen was strongly impaired in the presence of calcium or ethylenediaminetetraacetic acid (EDTA). Calcium 79-86 fibrinogen beta chain Homo sapiens 27-37 11313254-0 2001 Fibrinogen binding to the integrin alpha(IIb)beta(3) modulates store-mediated calcium entry in human platelets. Calcium 78-85 fibrinogen beta chain Homo sapiens 0-10 10748039-2 2000 We synthesized a variant, recombinant fibrinogen modeled after the heterozygous dysfibrinogen Vlissingen/Frankfurt IV, a deletion of two residues, gammaAsn-319 and gammaAsp-320, located within the high affinity calcium-binding pocket. Calcium 211-218 fibrinogen beta chain Homo sapiens 38-48 11282030-5 2001 The affinity of this receptor for its ligands vitronectin, fibronectin and fibrinogen can be modulated by the divalent cations magnesium, calcium and manganese. Calcium 138-145 fibrinogen beta chain Homo sapiens 75-85 10748039-6 2000 Plasmin cleavage of this fibrinogen was not changed by the presence of calcium or Gly-Pro-Arg-Pro, indicating that both the calcium-binding site and the "a" polymerization site were non-functional. Calcium 124-131 fibrinogen beta chain Homo sapiens 25-35 10843900-8 2000 This increase in intracellular free calcium concentration induced by MAb 1.9 was found to be dependent on the binding of fibrinogen to activated GPIIb/IIIa integrins, suggesting that MAb 1.9 causes Ca(2+) flux through the fibrinogen receptor complex. Calcium 36-43 fibrinogen beta chain Homo sapiens 121-131 10064005-4 1999 Polymerization of fibrin monomers derived from purified fibrinogen was delayed in the presence of either calcium or EDTA. Calcium 105-112 fibrinogen beta chain Homo sapiens 56-66 10843900-8 2000 This increase in intracellular free calcium concentration induced by MAb 1.9 was found to be dependent on the binding of fibrinogen to activated GPIIb/IIIa integrins, suggesting that MAb 1.9 causes Ca(2+) flux through the fibrinogen receptor complex. Calcium 36-43 fibrinogen beta chain Homo sapiens 222-232 10064005-9 1999 Three high-affinity calcium binding sites were found in both normal and variant fibrinogen. Calcium 20-27 fibrinogen beta chain Homo sapiens 80-90 9296274-10 1997 Chronic treatment with calcium antagonist nilvadipine brought about significant reductions in BPs and rheological parameters of whole-blood viscosities at low and middle shear rates, corrected blood viscosity (Ht 45%) at a low shear rate, and plasma viscosity without changes in serum albumin and plasma fibrinogen levels, as compared with normotensive individuals. Calcium 23-30 fibrinogen beta chain Homo sapiens 304-314 9401066-10 1997 Further analysis of the D domain of purified fibrinogen established that calcium binding to the high affinity site remained unaffected by the bulky carbohydrate side chain or negatively charged sialic acid residues. Calcium 73-80 fibrinogen beta chain Homo sapiens 45-55 9295325-0 1997 The polymerization pocket "a" within the carboxyl-terminal region of the gamma chain of human fibrinogen is adjacent to but independent from the calcium-binding site. Calcium 145-152 fibrinogen beta chain Homo sapiens 94-104 9295325-1 1997 The carboxyl-terminal region of the gamma chain of fibrinogen is involved in calcium binding, fibrin polymerization, factor XIIIa-mediated cross-linking, and binding to the platelet fibrin(ogen) receptor. Calcium 77-84 fibrinogen beta chain Homo sapiens 51-61 9182584-4 1997 Binding of phosphacan and neurocan to intact tenascin-C, and of phosphacan to the fibrinogen globe, is significantly increased in the presence of calcium. Calcium 146-153 fibrinogen beta chain Homo sapiens 82-92 8883274-0 1996 Normal binding of calcium to five fibrinogen variants with mutations in the carboxy terminal part of the gamma-chain. Calcium 18-25 fibrinogen beta chain Homo sapiens 34-44 8883274-2 1996 Each of the two carboxy terminal domains of normal fibrinogen contains one high-affinity calcium binding site that seems to be situated close to the polymerization site in the gamma-chain. Calcium 89-96 fibrinogen beta chain Homo sapiens 51-61 8883274-9 1996 In contrast, the abnormal fibrin polymerization of fibrinogen gamma 330 Asp-->Val was barely improved at increasing calcium concentrations. Calcium 119-126 fibrinogen beta chain Homo sapiens 51-61 8883274-5 1996 We investigated binding of calcium to fibrinogen and the effect of calcium on the clotting properties of five heterozygous fibrinogen variants showing normal thrombin-induced fibrinopeptide release but abnormal polymerization of fibrin monomers. Calcium 27-34 fibrinogen beta chain Homo sapiens 38-48 7631313-7 1995 At ionic strength 0.15 and at different calcium concentrations analysis by permeability showed the same results for fibrinogen Tampere as for normal gels. Calcium 40-47 fibrinogen beta chain Homo sapiens 116-126 7631313-10 1995 In fibrin gels from fibrinogen Tampere, the gamma-chain crosslinking was normal but the crosslinking of alpha-chains was delayed at ionic strength 0.2 and also at lower ionic strengths on lowering the calcium concentration. Calcium 201-208 fibrinogen beta chain Homo sapiens 20-30 8611703-0 1996 Changes in cytosolic calcium concentrations and cell morphology in single platelets adhered to fibrinogen-coated surface under flow. Calcium 21-28 fibrinogen beta chain Homo sapiens 95-105 8735140-0 1996 Binding of calcium ions and their effect on clotting of fibrinogen Milano III, a variant with truncated A alpha-chains. Calcium 11-18 fibrinogen beta chain Homo sapiens 56-66 8735140-2 1996 Normal human fibrinogen has three high-affinity calcium binding sites. Calcium 48-55 fibrinogen beta chain Homo sapiens 13-23 8735140-5 1996 In several abnormally clotting fibrinogen variants, the polymerisation defect was partially normalised following addition of calcium ions. Calcium 125-132 fibrinogen beta chain Homo sapiens 31-41 8735140-6 1996 In this study, we show normal binding of calcium to fibrinogen Milano III, a homozygous fibrinogen variant with truncated A alpha-chains (A alpha 452 Gly-Pro-Asp-->Trp-Ser-Stop). Calcium 41-48 fibrinogen beta chain Homo sapiens 52-62 7495409-2 1995 At man and dog the addition of calcium stabilized from 0.06 mM Ca2+ (2.0 molecules Ca2+/molecule Fibrinogen) certain X- Y- and D-fragments which were not or not in this extent generated by degradation without calcium. Calcium 31-38 fibrinogen beta chain Homo sapiens 97-107 7495075-0 1995 Calcium ion-dependent monoclonal antibody against human fibrinogen: preparation, characterization, and application to fibrinogen purification. Calcium 0-7 fibrinogen beta chain Homo sapiens 56-66 7495075-1 1995 We have produced a high-affinity monoclonal antibody classified as IgG1 with kappa-type light chains that recognizes the calcium ion(Ca2+)-dependent conformation of the D-domain of human fibrinogen. Calcium 121-128 fibrinogen beta chain Homo sapiens 187-197 7495075-0 1995 Calcium ion-dependent monoclonal antibody against human fibrinogen: preparation, characterization, and application to fibrinogen purification. Calcium 0-7 fibrinogen beta chain Homo sapiens 118-128 8400260-8 1993 We conclude that the substitution gamma 337 Asn-->Lys in fibrinogen Bern I is responsible for defective polymerization of fibrin monomers and for impaired protection by calcium against plasmic degradation. Calcium 169-176 fibrinogen beta chain Homo sapiens 57-67 7742784-2 1995 Since sialic acids at the terminal of the carbohydrate chains bound to fibrinogen are part of the low affinity calcium binding site necessary for polymerization, they are closely involved in the network structure of fibrin clots. Calcium 111-118 fibrinogen beta chain Homo sapiens 71-81 1431204-4 1992 EDTA is known to induce an irreversible dissociation of the platelet membrane glycoprotein(GP)IIb-IIIa, the alpha IIb beta 3 platelet-specific intergrin, a calcium-dependent heterodimer that serves as an inducible receptor for fibrinogen and is essential for platelet aggregation. Calcium 156-163 fibrinogen beta chain Homo sapiens 227-237 8401394-0 1993 Alteration of fibrinogen secondary structure by cis-diamminedichloroplatinum(II) and calcium protection. Calcium 85-92 fibrinogen beta chain Homo sapiens 14-24 8401394-8 1993 In a buffer containing calcium, however, the smaller fluorescence quenching and the smaller helix decline were observed in cis-DDP-treated-Fbg, even though the same level of S-S cleavage occurred. Calcium 23-30 fibrinogen beta chain Homo sapiens 139-142 8401394-9 1993 Calcium binding would protect Fbg in terms of structural alteration as well as S-S cleavage by both dithiothreitol (DTT) and cis-DDP. Calcium 0-7 fibrinogen beta chain Homo sapiens 30-33 1644841-1 1992 The platelet integrin, glycoprotein IIb-IIIa (GPIIb-IIIa), is a calcium-dependent heterodimer that binds fibrinogen, von Willebrand factor, and fibronectin after platelet activation. Calcium 64-71 fibrinogen beta chain Homo sapiens 105-115 1421580-4 1992 Stimulation of these cells either by adhesion to fibrinogen or with antiserum directed against alpha IIb beta 3 results in induction of calcium oscillations, followed by tyrosine phosphorylation of at least one protein of molecular weight approximately 125 kDa. Calcium 136-143 fibrinogen beta chain Homo sapiens 49-59 1324718-5 1992 The possibility of Ca(2+)-dependent changes in the rigidity or conformation of fibrinogen was excluded by examining the submicrosecond time-resolved fluorescence depolarization of 1-methylpyrene conjugates of the protein in the presence of different calcium concentrations. Calcium 250-257 fibrinogen beta chain Homo sapiens 79-89 1289495-4 1992 The result indicates that calcium, which has three high affinity sites on Fbg, protects disulfide bridges from the rupture. Calcium 26-33 fibrinogen beta chain Homo sapiens 74-77 1736899-0 1992 Purification and characterization by fast-atom-bombardment mass spectrometry of the polymorphonuclear-leucocyte-elastase-generated A alpha (1-21) fragment of fibrinogen from human blood after incubation with calcium ionophore A23187. Calcium 208-215 fibrinogen beta chain Homo sapiens 158-168 1575681-0 1992 Spiking in cytosolic calcium concentration in single fibrinogen-bound fura-2-loaded human platelets. Calcium 21-28 fibrinogen beta chain Homo sapiens 53-63 1733971-2 1992 Prolonged thrombin time was completely corrected by the addition of millimolar concentrations of calcium in a new abnormal fibrinogen, Osaka V. Analysis of lysyl endopeptidase digests of A alpha-, B beta-, or gamma-chains by high performance liquid chromatography, and the following amino acid sequence analysis of relevant peptides revealed that about 50% of the gamma-chain has a replacement of gamma-arginine 375 by glycine. Calcium 97-104 fibrinogen beta chain Homo sapiens 123-133 1733971-3 1992 When fibrinogen was digested with plasmin in the presence of millimolar concentration of calcium, the amount of fragment D1 was about 50% of the normal control, and the rest was further cleaved to fragment D2, D3, or D62 with an apparent Mr of 62,000. Calcium 89-96 fibrinogen beta chain Homo sapiens 5-15 1451594-4 1992 When platelets were stimulated with ADP, calcium ionophore A23187, or thrombin, fibrinogen binding to the platelet surface increased markedly. Calcium 41-48 fibrinogen beta chain Homo sapiens 80-90 1744509-6 1991 Similar experiments performed in the presence of EGTA and calcium showed proportional expression of the GPIIb-IIIa complex and platelet fibrinogen. Calcium 58-65 fibrinogen beta chain Homo sapiens 136-146 2346725-11 1990 It also had a high binding affinity and was capable of inhibiting the binding of radiolabelled fibrinogen to the activated platelets at physiological calcium concentrations. Calcium 150-157 fibrinogen beta chain Homo sapiens 95-105 2071611-0 1991 A congenitally abnormal fibrinogen (Vlissingen) with a 6-base deletion in the gamma-chain gene, causing defective calcium binding and impaired fibrin polymerization. Calcium 114-121 fibrinogen beta chain Homo sapiens 24-34 35583079-3 2022 Fine X-ray photoelectron spectroscopy and Fourier-transform infrared spectroscopy analyses reported calcium-dependent shifts of the binding energy in nitrogen and oxygen involved groups and wavenumbers in the amide I and II bands of the adsorbent fibrinogen, demonstrating that locally delivered calcium can react with the carboxy-terminal regions of the Aalpha chains and influence their interaction with the N-termini of the Bbeta chains in fibrinogen. Calcium 100-107 fibrinogen beta chain Homo sapiens 443-453 35583079-0 2022 The antimicrobial effect of calcium-doped titanium is activated by fibrinogen adsorption. Calcium 28-35 fibrinogen beta chain Homo sapiens 67-77 35583079-2 2022 Herein, we report an unexpected antimicrobial effect of calcium-doped titanium, which itself has no apparent killing effect on the growth of pathogenic bacteria (Pseudomonas aeruginosa, Pa, ATCC 27853) while presenting strong inhibition efficiency on bacterial colonization after fibrinogen adsorption onto the material. Calcium 56-63 fibrinogen beta chain Homo sapiens 280-290 2334711-0 1990 Disulfide bond reduction in fibrinogen: calcium protection and effect on clottability. Calcium 40-47 fibrinogen beta chain Homo sapiens 28-38 2334711-6 1990 N-Terminal analysis of thrombin-treated samples showed that thrombin cleavage occurred at the normal A alpha 16-A alpha 17 site in fibrinogen that was partially reduced in the presence of calcium. Calcium 188-195 fibrinogen beta chain Homo sapiens 131-141 35583079-3 2022 Fine X-ray photoelectron spectroscopy and Fourier-transform infrared spectroscopy analyses reported calcium-dependent shifts of the binding energy in nitrogen and oxygen involved groups and wavenumbers in the amide I and II bands of the adsorbent fibrinogen, demonstrating that locally delivered calcium can react with the carboxy-terminal regions of the Aalpha chains and influence their interaction with the N-termini of the Bbeta chains in fibrinogen. Calcium 100-107 fibrinogen beta chain Homo sapiens 247-257 35583079-3 2022 Fine X-ray photoelectron spectroscopy and Fourier-transform infrared spectroscopy analyses reported calcium-dependent shifts of the binding energy in nitrogen and oxygen involved groups and wavenumbers in the amide I and II bands of the adsorbent fibrinogen, demonstrating that locally delivered calcium can react with the carboxy-terminal regions of the Aalpha chains and influence their interaction with the N-termini of the Bbeta chains in fibrinogen. Calcium 296-303 fibrinogen beta chain Homo sapiens 247-257 2549051-0 1989 Fibrinogen sialic acid residues are low affinity calcium-binding sites that influence fibrin assembly. Calcium 49-56 fibrinogen beta chain Homo sapiens 0-10 2573600-5 1989 Calcium-dependent binding was completely inhibited by unlabeled fibrinogen, partially inhibited by a monoclonal antibody (7E3) against glycoprotein IIb-IIIa, but not inhibited by fibrinogen fragments D or E, an anti-glycoprotein IIIa polyclonal antibody, or the Arg-Gly-Asp-Ser tetrapeptide. Calcium 0-7 fibrinogen beta chain Homo sapiens 64-74 2691968-4 1989 The platelet receptor for fibrinogen is a macromolecular complex, GPIIb-IIIa, made of two transmembrane glycoproteins, GPIIb (Mr = 142,000) and GPIIIa (Mr = 99,000), assembled into a heterodimer whose conformation depends upon the binding of calcium (Ca2+) to GPIIb. Calcium 242-249 fibrinogen beta chain Homo sapiens 26-36 2549051-1 1989 Calcium ions occupy low (n congruent to 10; Kd congruent to 1 mM) and high (n = 3; Kd congruent to 1 microM) affinity sites on fibrinogen and facilitate fibrin monomer polymerization. Calcium 0-7 fibrinogen beta chain Homo sapiens 127-137 2923917-0 1989 The influence of calcium ions on fibrinogen conformation. Calcium 17-24 fibrinogen beta chain Homo sapiens 33-43 2923917-2 1989 The results obtained suggest that fibrinogen is a flexible molecule and its conformation is influenced by the concentration of calcium ions. Calcium 127-134 fibrinogen beta chain Homo sapiens 34-44 2923917-3 1989 These effects are mediated through binding to the low-affinity calcium binding sites of fibrinogen. Calcium 63-70 fibrinogen beta chain Homo sapiens 88-98 2484687-4 1989 SIN-1A acts as an early step of platelet activation by inhibiting calcium influx and phospholipase activity, which leads to inhibition of thromboxane formation and fibrinogen binding. Calcium 66-73 fibrinogen beta chain Homo sapiens 164-174 2833330-1 1988 Lack of requirement for fibrinogen during adenosine diphosphate-induced responses and enhanced fibrinogen binding in a medium with low calcium levels. Calcium 135-142 fibrinogen beta chain Homo sapiens 95-105 2954261-4 1987 The abnormal fibrinogen polymerized in the presence of calcium and can be further cross-linked by factor XIIIa. Calcium 55-62 fibrinogen beta chain Homo sapiens 13-23 3497679-0 1987 Effect of calcium ion concentration on the ability of fibrinogen and von Willebrand factor to support the ADP-induced aggregation of human platelets. Calcium 10-17 fibrinogen beta chain Homo sapiens 54-64 2958955-1 1987 The abnormal fibrinogen Haifa is characterized by the fact that calcium present during enzymatic digestion by plasmin does not protect the Haifa D gamma chain against further plasmin attack as it does in normal molecules. Calcium 64-71 fibrinogen beta chain Homo sapiens 13-23 3548842-1 1987 The platelet membrane glycoproteins IIb (GpIIb) and GpIIIa form calcium-dependent heterodimers containing binding sites for fibrinogen, von Willebrand factor, and fibronectin. Calcium 64-71 fibrinogen beta chain Homo sapiens 124-134 3365374-1 1988 Kinetic analysis of the release of fibrinopeptides by thrombin and of the calcium uptake upon clotting at high fibrinogen concentrations. Calcium 74-81 fibrinogen beta chain Homo sapiens 111-121 3216814-3 1988 In addition, the effect of galactose and calcium ions on the PC activity of these strains in experiments employing human fibrinogen permitted the recognition of these groups of S. aureus strains. Calcium 41-48 fibrinogen beta chain Homo sapiens 121-131 3497679-3 1987 In the low calcium medium, either vWf or fibrinogen supported biphasic aggregation in response to ADP, with thromboxane formation and release of granule contents. Calcium 11-18 fibrinogen beta chain Homo sapiens 41-51 3620452-6 1987 A delay in the onset of aggregation was also shown in calcium-lacking buffers by use of either reaggregating fibrin or fibrinogen aggregated with low (0.01-0.05 unit/mL) thrombin concentrations. Calcium 54-61 fibrinogen beta chain Homo sapiens 119-129 4052633-7 1985 In the presence of calcium, fibrinogen at concentrations from 0 to 12 mumol/L reduced the rate of inhibition in a competitive manner, giving an apparent Kd for fibrinogen of 6.0 mumol/L. Calcium 19-26 fibrinogen beta chain Homo sapiens 28-38 3743572-6 1986 Binding was specific for fibrinogen, was not observed with thrombasthenic platelets and was dependent upon the presence of extracellular calcium. Calcium 137-144 fibrinogen beta chain Homo sapiens 25-35 2941443-2 1986 Platelet membrane glycoproteins (GP) IIb and IIIa constitute a receptor for fibrinogen that, together with fibrinogen and calcium, is largely responsible for mediating the formation of the primary hemostatic plug. Calcium 122-129 fibrinogen beta chain Homo sapiens 76-86 2437513-1 1986 A simple method of concentrating a patient"s own fibrinogen for use with a thrombin/calcium solution--to produce a coagulum-type tissue glue for otologic surgery--is presented. Calcium 84-91 fibrinogen beta chain Homo sapiens 49-59 2937452-0 1986 Localization of segments essential for polymerization and for calcium binding in the gamma-chain of human fibrinogen. Calcium 62-69 fibrinogen beta chain Homo sapiens 106-116 4052633-7 1985 In the presence of calcium, fibrinogen at concentrations from 0 to 12 mumol/L reduced the rate of inhibition in a competitive manner, giving an apparent Kd for fibrinogen of 6.0 mumol/L. Calcium 19-26 fibrinogen beta chain Homo sapiens 160-170 3161453-1 1985 Human, dog, and rabbit fibrinogen served as substrates for calcium-activated, phospholipid-dependent protein kinase, cAMP-dependent protein kinase, casein kinase TS, and casein kinase S. The chains of phosphorylated fibrinogen were separated by polyacrylamide gel electrophoresis and the phosphorylation patterns, obtained on autoradiography of the gels, were found to be characteristic for each of the four protein kinases. Calcium 59-66 fibrinogen beta chain Homo sapiens 23-33 2932434-2 1985 This part of the molecule is retained on the gamma chain of fragment D (FgD) when fibrinogen is digested by plasmin in the presence of calcium to produce the fragment D-fragment E (FgD X FgE) complex but is lost if FgD is prepared in the absence of calcium. Calcium 135-142 fibrinogen beta chain Homo sapiens 82-92 2932434-2 1985 This part of the molecule is retained on the gamma chain of fragment D (FgD) when fibrinogen is digested by plasmin in the presence of calcium to produce the fragment D-fragment E (FgD X FgE) complex but is lost if FgD is prepared in the absence of calcium. Calcium 249-256 fibrinogen beta chain Homo sapiens 82-92 3160702-0 1985 Localization of a fibrinogen calcium binding site between gamma-subunit positions 311 and 336 by terbium fluorescence. Calcium 29-36 fibrinogen beta chain Homo sapiens 18-28 3983914-1 1985 Factor XIII induced gelation of human fibrinogen in the presence of calcium ions. Calcium 68-75 fibrinogen beta chain Homo sapiens 38-48 3929831-3 1985 When clotting is effected by thrombin in the presence of calcium, the endotherm for the D nodules of fibrinogen broadens significantly and then becomes narrow again, while increasing in size. Calcium 57-64 fibrinogen beta chain Homo sapiens 101-111 3158281-1 1985 Calcium limits the plasmic proteolysis of fibrinogen fragment D by binding to a specific site on the carboxy-terminal segment of the D gamma chain. Calcium 0-7 fibrinogen beta chain Homo sapiens 42-52 3983914-11 1985 Preincubation of fibrinogen with calcium ions prevented this effect of DTT. Calcium 33-40 fibrinogen beta chain Homo sapiens 17-27 6612699-1 1983 Human fibrinogen has three calcium-binding sites. Calcium 27-34 fibrinogen beta chain Homo sapiens 6-16 6729777-5 1984 In addition, the effects of citrate on the clotting of purified, calcium-free fibrinogen from cirrhotic patients correlated with the sialic acid content. Calcium 65-72 fibrinogen beta chain Homo sapiens 78-88 6320488-1 1983 Platelets deprived of calcium and incubated at 37 degrees C for 10 min lose their ability to bind fibrinogen or aggregate with ADP when adequate concentrations of calcium are restored. Calcium 22-29 fibrinogen beta chain Homo sapiens 98-108 6320488-2 1983 Since the calcium complex of glycoproteins (GP) IIb and IIIa is the presumed receptor for fibrinogen, it seemed appropriate to examine the behavior of these glycoproteins in incubated nonaggregable platelets. Calcium 10-17 fibrinogen beta chain Homo sapiens 90-100 6238443-2 1984 On digestion with leukocyte elastase, in the presence of calcium ions, the anticoagulant potency of fibrinogen digests first increases, then decreases sharply, and in late stages increases again. Calcium 57-64 fibrinogen beta chain Homo sapiens 100-110 6658718-5 1983 The accelerating effect of calcium on the thrombin clotting time was more pronounced for newborn cord plasma and for purified cord fibrinogen preparations as compared with adult fibrinogen. Calcium 27-34 fibrinogen beta chain Homo sapiens 131-141 6658718-5 1983 The accelerating effect of calcium on the thrombin clotting time was more pronounced for newborn cord plasma and for purified cord fibrinogen preparations as compared with adult fibrinogen. Calcium 27-34 fibrinogen beta chain Homo sapiens 178-188 6309797-5 1983 The data in the present study suggest that there are two divalent cation binding sites that must be occupied for fibrinogen to bind: one site is specific for calcium and is saturated at 10(-6) M Ca2+; the other site is less specific and is saturated at a 10(-3) M concentration of either Ca2+ or Mg2+. Calcium 158-165 fibrinogen beta chain Homo sapiens 113-123 6410532-6 1983 More fibrinogen associated with platelets in the medium without calcium than in the calcium-containing medium. Calcium 64-71 fibrinogen beta chain Homo sapiens 5-15 7209542-0 1981 Influence of calcium ion on the binding of fibrin amino terminal peptides to fibrinogen. Calcium 13-20 fibrinogen beta chain Homo sapiens 77-87 6216917-0 1982 Anticoagulant and calcium-binding properties of high molecular weight derivatives of human fibrinogen (plasmin fragments Y). Calcium 18-25 fibrinogen beta chain Homo sapiens 91-101 6216917-1 1982 The present study was undertaken as a step to delineate further the localization of the calcium-binding sites in fibrinogen and to assess the anticlotting properties of fibrinogen degradation products. Calcium 88-95 fibrinogen beta chain Homo sapiens 113-123 7138883-0 1982 A fibrinogen fragment D (D intermediate) with calcium binding but without anticlotting properties. Calcium 46-53 fibrinogen beta chain Homo sapiens 2-12 6460044-3 1982 The formation of this GPIIb-GPIIIa fibrinogen complex is calcium dependent, fibrinogen specific, saturable, and inhibited by specific amino sugars and amino acids. Calcium 57-64 fibrinogen beta chain Homo sapiens 35-45 6460044-3 1982 The formation of this GPIIb-GPIIIa fibrinogen complex is calcium dependent, fibrinogen specific, saturable, and inhibited by specific amino sugars and amino acids. Calcium 57-64 fibrinogen beta chain Homo sapiens 76-86 7209542-1 1981 The affinity of the amino terminal tetrapeptide of the beta chain of fibrin, Gly-His-Arg-Pro, for fibrinogen dramatically increases in the presence of 2 millimolar calcium ion. Calcium 164-171 fibrinogen beta chain Homo sapiens 98-108 6288701-5 1982 Fibrinogen was digested with plasmin in the presence of 5 mM calcium and fragment D and E of Mr 100,000 and 50,000, respectively, were isolated. Calcium 61-68 fibrinogen beta chain Homo sapiens 0-10 6954514-5 1982 The inhibition was dependent upon fibrinogen concentration and occurred in the presence of calcium or magnesium. Calcium 91-98 fibrinogen beta chain Homo sapiens 34-44 7066337-0 1982 Potential role of the A alpha chain in the binding of calcium to human fibrinogen. Calcium 54-61 fibrinogen beta chain Homo sapiens 71-81 6456216-1 1981 Influence of calcium on the generation of fibrinogen degradation products with anticlotting properties. Calcium 13-20 fibrinogen beta chain Homo sapiens 42-52 6456216-2 1981 Degradation of human fibrinogen by elastase-like proteinase, chymotrypsin-like proteinase and plasmin, was done in the presence and absence of calcium ions, respectively. Calcium 143-150 fibrinogen beta chain Homo sapiens 21-31 6456216-7 1981 The fibrinogen fragments obtained in the presence of calcium are different in their molecular weights and anticoagulant activities compared to those obtained in the absence of calcium ions. Calcium 53-60 fibrinogen beta chain Homo sapiens 4-14 6456216-7 1981 The fibrinogen fragments obtained in the presence of calcium are different in their molecular weights and anticoagulant activities compared to those obtained in the absence of calcium ions. Calcium 176-183 fibrinogen beta chain Homo sapiens 4-14 6456216-8 1981 This effect of calcium is most striking during fibrinogen cleavage by chymotrypsin-like proteinase. Calcium 15-22 fibrinogen beta chain Homo sapiens 47-57 7040863-3 1981 Biogenic glue consists of fibrinogen, factor XIII, and thrombin, which form a fibrin clot in the presence of calcium. Calcium 109-116 fibrinogen beta chain Homo sapiens 26-36 6453618-0 1981 Anticoagulant and calcium-binding properties of high molecular weight derivatives of human fibrinogen, produced by plasmin (fragments X). Calcium 18-25 fibrinogen beta chain Homo sapiens 91-101 6452172-2 1981 The previously described protective effect of calcium ions on the gamma-chain carboxy-terminals of fibrinogen against attack has been confirmed by working at high plasmin concentrations and/or in the presence of 2 M urea. Calcium 46-53 fibrinogen beta chain Homo sapiens 99-109 478136-0 1979 Purification of fibrinogen and the separation of its degradation products in the presence of calcium ions [proceedings]. Calcium 93-100 fibrinogen beta chain Homo sapiens 16-26 7391026-1 1980 Human fibrinogen was treated with thrombin in the presence of fibrinoligase and calcium ion at pH 8.5, ionic strength 0.45, and the ensuring polymerization was interrupted at various time intervals (t) both before and after the clotting time (tc) by solubilization with a solution of sodium dodecyl sulfate and urea. Calcium 80-87 fibrinogen beta chain Homo sapiens 6-16 148750-0 1978 Stabilization of the plasmin digestion products of fibrinogen and fibrin by calcium ions. Calcium 76-83 fibrinogen beta chain Homo sapiens 51-61 465525-0 1979 The binding of calcium to fibrinogen: influence on the clotting process. Calcium 15-22 fibrinogen beta chain Homo sapiens 26-36 687825-2 1978 With this technique the effect of calcium on the three distinguishable phases of clot formation, (1) proteolysis of fibrinogen, (2) fibrinogen-fibrin monomer complex formation, and (3) fibrin monomer polymerization, were investigated. Calcium 34-41 fibrinogen beta chain Homo sapiens 116-126 687825-2 1978 With this technique the effect of calcium on the three distinguishable phases of clot formation, (1) proteolysis of fibrinogen, (2) fibrinogen-fibrin monomer complex formation, and (3) fibrin monomer polymerization, were investigated. Calcium 34-41 fibrinogen beta chain Homo sapiens 132-142 687825-5 1978 These data show that calcium decreases the time required for fibrin formation from fibrinogen by markedly accelerating the phase of fibrin monomer polymerization. Calcium 21-28 fibrinogen beta chain Homo sapiens 83-93 421898-0 1979 Calcium-binding properties of human fibrin(ogen) and degradation products. Calcium 0-7 fibrinogen beta chain Homo sapiens 36-48 741431-0 1978 Calcium binding to human fibrinogen - localization of two calcium specific sites. Calcium 0-7 fibrinogen beta chain Homo sapiens 25-35 741431-0 1978 Calcium binding to human fibrinogen - localization of two calcium specific sites. Calcium 58-65 fibrinogen beta chain Homo sapiens 25-35 11344575-4 1974 The thrombin time of plasma or purified fibrinogen was prolonged and only partially corrected by the addition of calcium. Calcium 113-120 fibrinogen beta chain Homo sapiens 40-50 20154-0 1977 The binding of calcium to fibrinogen: some structural features. Calcium 15-22 fibrinogen beta chain Homo sapiens 26-36 20154-1 1977 Experiments are described which suggest that structural features are related to the existence of three high affinity calcium-binding sites in the fibrinogen molecule. Calcium 117-124 fibrinogen beta chain Homo sapiens 146-156 20154-4 1977 At pH 5.0 calcium-free fibrinogen is slightly acid-denatured. Calcium 10-17 fibrinogen beta chain Homo sapiens 23-33 20154-9 1977 Resistance to proteolysis by plasmin is observed when calcium is bound to fibrinogen. Calcium 54-61 fibrinogen beta chain Homo sapiens 74-84 20154-12 1977 And that the calcium divalent cation stabilizes a more compact structure of the fibrinogen molecule. Calcium 13-20 fibrinogen beta chain Homo sapiens 80-90 142315-0 1977 Protective effect of calcium in the plasmin degradation of fibrinogen and fibrin fragments D. Calcium 21-28 fibrinogen beta chain Homo sapiens 59-69 1258156-0 1976 [Anticlotting activity of fragment D from fibrinogen and fibrin and its dependence on calcium presence when fragments are obtained from fibrinogen]. Calcium 86-93 fibrinogen beta chain Homo sapiens 136-146 1133167-8 1975 If clotting was carried out with calcium concentrations twice that required for normal clots or at pH 6.4, fibrin from fibrinogen Baltimore was completely cross-linked. Calcium 33-40 fibrinogen beta chain Homo sapiens 119-129 4856883-6 1974 The abnormally long thrombin time of fibrinogen Cleveland II was partially corrected by addition of calcium ions. Calcium 100-107 fibrinogen beta chain Homo sapiens 37-47 14830360-0 1951 [The effect, in the presence and absence of ionized calcium, of certain antagonists of the thrombin conversion of fibrinogen]. Calcium 52-59 fibrinogen beta chain Homo sapiens 114-124 4985342-0 1970 [Role of calcium in the structure of fibrinogen]. Calcium 9-16 fibrinogen beta chain Homo sapiens 37-47 13130687-0 1954 The accelerating effect of calcium and other cations on the conversion of fibrinogen to fibrin. Calcium 27-34 fibrinogen beta chain Homo sapiens 74-84 14298739-0 1964 [EFFECT OF CALCIUM, IONIC STRENGTH AND TEMPERATURE ON THE ENZYMATIC PHASE OF FIBRINOGEN CONVERSION TO FIBRIN]. Calcium 11-18 fibrinogen beta chain Homo sapiens 77-87 19872865-6 1935 Calcium, a platelet factor, and a plasma factor (prothrombin) interact to form thrombin, and this then acts upon fibrinogen to form fibrin. Calcium 0-7 fibrinogen beta chain Homo sapiens 113-123 18129500-0 1949 Influence of serum proteins and calcium ions upon the velocity of the reaction thrombin fibrinogen; second phase of blood coagulation. Calcium 32-39 fibrinogen beta chain Homo sapiens 88-98 26729371-8 2016 Ionised calcium concentrations and its interaction term with platelet count were both significantly associated with the MA on the TEG (slope of the regression line 1 1 per 0 1 mmol L(-1) increment, 95%CI 0 3 to 1 9, P = 0 011), after adjusting for fibrinogen concentrations, platelet counts, INR and aPTT. Calcium 8-15 fibrinogen beta chain Homo sapiens 248-258 33276061-4 2021 RESULTS: Large increases in Tm indicated that calcium led to protein stabilization (0 vs. 2 mM Ca2+) for fibrinogen (54.0 vs. 62.3 C) and fibrin (62.3 vs. 72.2 C). Calcium 46-53 fibrinogen beta chain Homo sapiens 105-115 28057772-6 2017 We also purified fibrinogen from healthy controls and patients on hemodialysis using the calcium-dependent IF-1 mAb against fibrinogen for additional investigation using mass spectrometric analysis and electron microscopy. Calcium 89-96 fibrinogen beta chain Homo sapiens 124-134 27310988-0 2016 Association of serum calcium concentrations with fibrinogen and homocysteine in nondiabetic Korean subjects. Calcium 21-28 fibrinogen beta chain Homo sapiens 49-59 27310988-2 2016 This study investigated whether serum calcium, within a normal range, is significantly associated with serum fibrinogen and homocysteine, markers of increased cardiovascular disease risk in nondiabetic Korean subjects.A cross-sectional analysis was performed on 1096 subjects (mean age, 55.1 +- 11.1 years; 36.1% women) undergoing a general health checkup. Calcium 38-45 fibrinogen beta chain Homo sapiens 109-119 29869737-5 2018 The polymerization of the purified fibrinogen was strongly impaired in the presence of calcium. Calcium 87-94 fibrinogen beta chain Homo sapiens 35-45 26826315-5 2016 Functional binding assays demonstrated calcium ion dependent interaction of Fibulin-1 for fibrinogen, fibronectin, and Factor H. Calcium 39-46 fibrinogen beta chain Homo sapiens 90-100 25955503-4 2015 The results revealed a typical behavior due to coagulation induced by calcium ions and the clotting time is Fbg concentration-dependent. Calcium 70-77 fibrinogen beta chain Homo sapiens 108-111 18564219-5 2008 That singular observation led to our hypothesis that native plasma factor XIII (FXIII), which is known to catalyze covalent cross-linking of fibrinogen in the presence of calcium ions, can also incorporate alpha2AP into fibrinogen in the circulation. Calcium 171-178 fibrinogen beta chain Homo sapiens 141-151 20731339-0 2010 Calcium dependence of fibrin nanomechanics: the gamma1 calcium mediates the unfolding of fibrinogen induced by force applied to the "A-a" bond. Calcium 0-7 fibrinogen beta chain Homo sapiens 89-99 20731339-0 2010 Calcium dependence of fibrin nanomechanics: the gamma1 calcium mediates the unfolding of fibrinogen induced by force applied to the "A-a" bond. Calcium 55-62 fibrinogen beta chain Homo sapiens 89-99 20731339-6 2010 Rather, calcium bound at the gamma1 site makes the structure of the hole more resilient to such forced unfolding, leading to survival of the "A-a" knob-hole bond during larger extensions of the fibrinogen molecule but at the cost of rupture of the bond at lower forces. Calcium 8-15 fibrinogen beta chain Homo sapiens 194-204 18294856-7 2008 AFM data show that varying pH and calcium ion concentrations alters the mechanical resilience of fibrinogen. Calcium 34-41 fibrinogen beta chain Homo sapiens 97-107 17854865-4 2008 Polymerization of fibrin monomers derived from patient fibrinogen was severely impaired with a partial correction in the presence of calcium ions, causing very low clottability and delayed cross-linking of patient fibrin catalyzed by activated factor XIII. Calcium 133-140 fibrinogen beta chain Homo sapiens 55-65 17854865-6 2008 Additionally, tPA-mediated plasmin generation on fibrin was impaired and calcium-ion-dependent integrity of the gamma-chain D domain of Kagoshima fibrinogen was perturbed. Calcium 73-80 fibrinogen beta chain Homo sapiens 146-156