PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 21297999-2 2011 This process can be recapitulated using recombinant Apaf-1 and CC in the presence of nucleotides ATP or dATP [(d)ATP] or using fresh cytosol and CC without the need of exogenous nucleotides. 2'-deoxyadenosine triphosphate 104-108 cytochrome c, somatic Homo sapiens 63-65 21463108-6 2011 Both dATP and CdATP cause an initial accumulation of DNA strand breaks in lymphocytes and this results in the activation of p53, the release of cytochrome c from mitochondria, and apoptosis. 2'-deoxyadenosine triphosphate 5-9 cytochrome c, somatic Homo sapiens 144-156 19333786-5 2008 The model predicts that the activation begins with binding of dATP to Apaf-1, which initiates the interaction between Apaf-1 and cytochrome c, thus forming a complex that oligomerizes into an active caspase-9 holoenzyme via a linear binding model with cooperative interaction rather than through network formation. 2'-deoxyadenosine triphosphate 62-66 cytochrome c, somatic Homo sapiens 129-141 18439902-2 2008 The Apaf-1/cytochrome c complex then oligomerizes either into heptameric caspase-9-activating apoptosome, which subsequently activates caspase-3 and caspase-7, or bigger inactive aggregates, depending on the availability of nucleotide dATP/ATP. 2'-deoxyadenosine triphosphate 235-239 cytochrome c, somatic Homo sapiens 11-23 15794635-4 2005 In the presence of cytochrome c (CytC), dATP or ATP binds to Apaf-1 and triggers heptamerization of Apaf-1 leading to the activation of Csp9. 2'-deoxyadenosine triphosphate 40-44 cytochrome c, somatic Homo sapiens 19-31 16271896-1 2005 Apaf-1 and cytochrome c coassemble in the presence of dATP to form the apoptosome. 2'-deoxyadenosine triphosphate 54-58 cytochrome c, somatic Homo sapiens 11-23 15794635-4 2005 In the presence of cytochrome c (CytC), dATP or ATP binds to Apaf-1 and triggers heptamerization of Apaf-1 leading to the activation of Csp9. 2'-deoxyadenosine triphosphate 40-44 cytochrome c, somatic Homo sapiens 33-37 11035811-0 2000 Cytochrome c binding to Apaf-1: the effects of dATP and ionic strength. 2'-deoxyadenosine triphosphate 47-51 cytochrome c, somatic Homo sapiens 0-12 15101558-6 2004 By contrast, cytochrome c plus dATP could induce a significant increase of caspase-3-like activity in cytosol only in some NSCLC cell lines and in subsets of studied NSCLC tissues and lungs, while procaspase-3 and -7 were detectably processed only in NSCLC tissues which showed a high (cytochrome c+dATP)-induced caspase-3-like activity. 2'-deoxyadenosine triphosphate 31-35 cytochrome c, somatic Homo sapiens 286-298 15101558-6 2004 By contrast, cytochrome c plus dATP could induce a significant increase of caspase-3-like activity in cytosol only in some NSCLC cell lines and in subsets of studied NSCLC tissues and lungs, while procaspase-3 and -7 were detectably processed only in NSCLC tissues which showed a high (cytochrome c+dATP)-induced caspase-3-like activity. 2'-deoxyadenosine triphosphate 299-303 cytochrome c, somatic Homo sapiens 13-25 11264002-1 2001 Apaf-1 plays a crucial role in the cytochrome c/dATP-dependent activation of caspase-9 and -3. 2'-deoxyadenosine triphosphate 48-52 cytochrome c, somatic Homo sapiens 35-47 11230124-1 2001 During apoptosis, release of cytochrome c initiates dATP-dependent oligomerization of Apaf-1 and formation of the apoptosome. 2'-deoxyadenosine triphosphate 52-56 cytochrome c, somatic Homo sapiens 29-41 11035811-3 2000 Here, we show through fluorescence polarization experiments that cytochrome c can bind to Apaf-1 in the absence of dATP; when dATP is added to the cytochrome c.Apaf-1 complex, further assembly occurs to produce the apoptosome. 2'-deoxyadenosine triphosphate 115-119 cytochrome c, somatic Homo sapiens 65-77 11035811-3 2000 Here, we show through fluorescence polarization experiments that cytochrome c can bind to Apaf-1 in the absence of dATP; when dATP is added to the cytochrome c.Apaf-1 complex, further assembly occurs to produce the apoptosome. 2'-deoxyadenosine triphosphate 126-130 cytochrome c, somatic Homo sapiens 65-77 11035811-3 2000 Here, we show through fluorescence polarization experiments that cytochrome c can bind to Apaf-1 in the absence of dATP; when dATP is added to the cytochrome c.Apaf-1 complex, further assembly occurs to produce the apoptosome. 2'-deoxyadenosine triphosphate 126-130 cytochrome c, somatic Homo sapiens 147-159 12439591-6 2002 The caspase cascade is activated by the release of cytochrome c, which is initiated by the formation of apoptosomes consisting of procaspase-9, Apaf-1 and cytochrome c in the presence of dATP, and results in the activation of caspase-9 and caspase-3, thereby leading to apoptosis. 2'-deoxyadenosine triphosphate 187-191 cytochrome c, somatic Homo sapiens 51-63 11520805-2 2001 Once released, cytochrome c cooperates with apoptotic protease-activating factor-1 and deoxyadenosine triphosphate in caspase-9 activation and initiation of the apoptotic protease cascade. 2'-deoxyadenosine triphosphate 87-114 cytochrome c, somatic Homo sapiens 15-27 10940292-5 2000 Procaspase-9 also synergistically promotes dATP binding to Apaf-1 in a cytochrome c-dependent manner. 2'-deoxyadenosine triphosphate 43-47 cytochrome c, somatic Homo sapiens 71-83 10940292-8 2000 These data indicate that the key event in Apaf-1-mediated caspase-9 activation is cytochrome c-induced dATP binding to Apaf-1. 2'-deoxyadenosine triphosphate 103-107 cytochrome c, somatic Homo sapiens 82-94 10722681-2 2000 Apaf-1 is an important apoptotic signaling molecule that can activate procaspase-9 in a cytochrome c/dATP-dependent fashion. 2'-deoxyadenosine triphosphate 101-105 cytochrome c, somatic Homo sapiens 88-100 10934467-1 2000 Release of cytochrome c from mitochondria by apoptotic signals induces ATP/dATP-dependent formation of the oligomeric Apaf-1-caspase-9 apoptosome. 2'-deoxyadenosine triphosphate 75-79 cytochrome c, somatic Homo sapiens 11-23 10393175-4 1999 We used this Apaf-1 to show that Apaf-1 requires dATP/ATP hydrolysis to interact with cytochrome c, self-associate and bind to procaspase-9. 2'-deoxyadenosine triphosphate 49-53 cytochrome c, somatic Homo sapiens 86-98 10687948-2 2000 When cells receive apoptotic stimuli, mitochondria releases cytochrome c which then binds to Apaf-1, the mammalian Ced-4 homologue, together with dATP. 2'-deoxyadenosine triphosphate 146-150 cytochrome c, somatic Homo sapiens 60-72 10617581-2 2000 We previously demonstrated that that the 5"-triphosphate metabolite of 2CdA (2CdATP), similar to dATP, can cooperate with cytochrome c and apoptosis protein-activating factor-1 (APAF-1) to trigger a caspase pathway in a HeLa cell-free system. 2'-deoxyadenosine triphosphate 79-83 cytochrome c, somatic Homo sapiens 122-134 10544189-9 1999 We conclude that HSP27 inhibits etoposide-induced apoptosis by preventing cytochrome c and dATP-triggered activity of caspase-9, downstream of cytochrome c release. 2'-deoxyadenosine triphosphate 91-95 cytochrome c, somatic Homo sapiens 143-155 10206961-3 1999 The hydrolysis of ATP/dATP and the binding of cytochrome c promote APAF-1 oligomerization, forming a large multimeric APAF-1.cytochrome c complex. 2'-deoxyadenosine triphosphate 22-26 cytochrome c, somatic Homo sapiens 125-137 10364241-4 1999 However, when dATP is added to the cytochrome c-bound Apaf-1 complex, complete oligomerization occurs, suggesting that oligomerization is driven by hydrolysis of dATP. 2'-deoxyadenosine triphosphate 14-18 cytochrome c, somatic Homo sapiens 35-47 10364241-4 1999 However, when dATP is added to the cytochrome c-bound Apaf-1 complex, complete oligomerization occurs, suggesting that oligomerization is driven by hydrolysis of dATP. 2'-deoxyadenosine triphosphate 162-166 cytochrome c, somatic Homo sapiens 35-47 9753651-4 1998 The dATP-dependent and swelling-independent release of cytochrome c from mitochondria is not inhibitable by the protease inhibitor z-VAD, suggesting that it is not mediated by upstream caspases. 2'-deoxyadenosine triphosphate 4-8 cytochrome c, somatic Homo sapiens 55-67 10611963-9 1999 This pathway is regulated at several steps, including the release of cytochrome c from the mitochondria, the binding and hydrolysis of dATP/ATP by Apaf-1, and the inhibition of caspase activation by the proteins that belong to the inhibitors of apoptosis (IAP). 2'-deoxyadenosine triphosphate 135-139 cytochrome c, somatic Homo sapiens 69-81 9753651-0 1998 dATP causes specific release of cytochrome C from mitochondria. 2'-deoxyadenosine triphosphate 0-4 cytochrome c, somatic Homo sapiens 32-44 9753651-3 1998 By contrast, it was observed that dATP is a potent inducer that caused release of cytochrome c in a swelling independent manner, i.e. even in the presence of osmotic support by PEG-1000; in addition this release of cytochrome c is inhibitable by cyclosporin A. 2'-deoxyadenosine triphosphate 34-38 cytochrome c, somatic Homo sapiens 82-94 9753651-3 1998 By contrast, it was observed that dATP is a potent inducer that caused release of cytochrome c in a swelling independent manner, i.e. even in the presence of osmotic support by PEG-1000; in addition this release of cytochrome c is inhibitable by cyclosporin A. 2'-deoxyadenosine triphosphate 34-38 cytochrome c, somatic Homo sapiens 215-227 9689121-3 1998 The present experiments demonstrate that the 5"-triphosphate metabolite of 2CdA (2CdA-5"-triphosphate), similar to dATP, can cooperate with cytochrome c and Apaf-1 to activate caspase-3 in a cell free system. 2'-deoxyadenosine triphosphate 115-119 cytochrome c, somatic Homo sapiens 140-152 9390557-6 1997 Mutation of the active site of caspase-9 attenuated the activation of caspase-3 and cellular apoptotic response in vivo, indicating that caspase-9 is the most upstream member of the apoptotic protease cascade that is triggered by cytochrome c and dATP. 2'-deoxyadenosine triphosphate 247-251 cytochrome c, somatic Homo sapiens 230-242 24626292-4 2014 Further analysis demonstrated formation of high-Mr Apaf-1 complexes associated with cleaved caspase-9 in the (cyt-c + dATP)-responsive COLO-699 and CALU-1 cells. 2'-deoxyadenosine triphosphate 118-122 cytochrome c, somatic Homo sapiens 110-115 24626292-10 2014 Taken together, the present study provides evidence that the apoptosome apparatus is functional in the majority of NSCLCs and that its sensitivity to the (cyt-c + dATP)-mediated activation is often enhanced in NSCLCs compared to lungs. 2'-deoxyadenosine triphosphate 163-167 cytochrome c, somatic Homo sapiens 155-160