PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 22626465-9 2012 This may be associated with the significant suppression of PGE(2) /IL-8 secretion via the down-regulated expression of COX-2 and IL-8 at the gene and/or protein levels. Prostaglandins E 59-62 C-X-C motif chemokine ligand 8 Homo sapiens 67-71 22517618-5 2012 Using selective EP receptor agonists and a selective EP(4) antagonist, we show that PGE(2) mediates the repression of IL-1beta-induced release of CXCL8, CCL2, and CSF2 via activation of the EP(2) and EP(4) receptors. Prostaglandins E 84-87 C-X-C motif chemokine ligand 8 Homo sapiens 146-151 22924768-1 2013 BACKGROUND AND PURPOSE: Recent studies suggested a role for PGE(2) in the expression of the chemokine IL-8. Prostaglandins E 60-63 C-X-C motif chemokine ligand 8 Homo sapiens 103-107 22924768-5 2013 KEY RESULTS: In HEK-EP(1) and HEK-EP(1) + EP(4) but not HEK or HEK-EP(4) cells, PGE(2) activated the IL-8 promoter and induced IL-8 mRNA and protein synthesis. Prostaglandins E 84-87 C-X-C motif chemokine ligand 8 Homo sapiens 106-110 22924768-5 2013 KEY RESULTS: In HEK-EP(1) and HEK-EP(1) + EP(4) but not HEK or HEK-EP(4) cells, PGE(2) activated the IL-8 promoter and induced IL-8 mRNA and protein synthesis. Prostaglandins E 84-87 C-X-C motif chemokine ligand 8 Homo sapiens 132-136 22626465-9 2012 This may be associated with the significant suppression of PGE(2) /IL-8 secretion via the down-regulated expression of COX-2 and IL-8 at the gene and/or protein levels. Prostaglandins E 59-62 C-X-C motif chemokine ligand 8 Homo sapiens 129-133 21798286-4 2011 However, HuMCs from the remaining donors and the LAD2 human MC line responded to PGE(2) and sulprostone with marked enhancement of antigen-mediated degranulation and IL-8 production in a similar manner to that observed in mouse BMMCs. Prostaglandins E 81-84 C-X-C motif chemokine ligand 8 Homo sapiens 166-170 22531917-3 2012 Here we determined the mechanisms whereby PGE(2) regu-lates IL-8 in Caco2 colonic epithelial cells and in cells over-expressing the EP2/4 receptors (EP2S/EP4S). Prostaglandins E 42-45 C-X-C motif chemokine ligand 8 Homo sapiens 60-64 22531917-7 2012 PGE(2) coupling through EP2/4 receptors can therefore acts in an opposing manner to either decrease (EP2) or promote IL-8 expression by recruiting CREB-binding protein (CBP) (EP4), which formed a multiprotein IL-8 enhanceosome. Prostaglandins E 0-3 C-X-C motif chemokine ligand 8 Homo sapiens 117-121 22531917-7 2012 PGE(2) coupling through EP2/4 receptors can therefore acts in an opposing manner to either decrease (EP2) or promote IL-8 expression by recruiting CREB-binding protein (CBP) (EP4), which formed a multiprotein IL-8 enhanceosome. Prostaglandins E 0-3 C-X-C motif chemokine ligand 8 Homo sapiens 209-213 22080750-2 2012 This study investigated whether PGE(2) can induce production of interleukin (IL)-8, the major chemokine for neutrophil activation, from human pulmonary microvascular endothelial cells (HPMVECs). Prostaglandins E 32-36 C-X-C motif chemokine ligand 8 Homo sapiens 64-82 22080750-3 2012 PGE(2) significantly enhanced IL-8 protein production with increases in IL-8 mRNA expression and intracellular cAMP levels. Prostaglandins E 0-3 C-X-C motif chemokine ligand 8 Homo sapiens 30-34 22080750-3 2012 PGE(2) significantly enhanced IL-8 protein production with increases in IL-8 mRNA expression and intracellular cAMP levels. Prostaglandins E 0-3 C-X-C motif chemokine ligand 8 Homo sapiens 72-76 22080750-9 2012 In conclusion, PGE(2) enhances IL-8 production via EP4 receptor coupled to G(s) protein in HPMVECs. Prostaglandins E 15-18 C-X-C motif chemokine ligand 8 Homo sapiens 31-35 22080750-11 2012 Because neutrophils play a critical role in the inflammation of acute lung injury/acute respiratory distress syndrome, IL-8 released from the pulmonary microvasculature in response to PGE(2) may contribute to pathophysiology of this disease. Prostaglandins E 184-187 C-X-C motif chemokine ligand 8 Homo sapiens 119-123 21356375-7 2011 IL-8 was increased by PGE(2) and hypoxia in secretory but not proliferative explants, which suggests that exposure to progesterone is essential. Prostaglandins E 22-25 C-X-C motif chemokine ligand 8 Homo sapiens 0-4 21356375-9 2011 Epithelial cells treated simultaneously with PGE(2) and hypoxia demonstrated synergistic increases in IL-8. Prostaglandins E 45-48 C-X-C motif chemokine ligand 8 Homo sapiens 102-106 21356375-10 2011 Inhibition of HIF-1 by short hairpin RNA abolished hypoxic IL-8 induction, and inhibition of NF-kappaB by an adenoviral dominant negative inhibitor decreased PGE(2)-induced IL-8 expression (P > 0.05). Prostaglandins E 158-161 C-X-C motif chemokine ligand 8 Homo sapiens 173-177 18506884-6 2008 The mutually antagonistic relationship between IFN-gamma and PGE(2) extends to downstream cytokine and chemokine release; PGE(2) counters the effects of IFN-gamma, on the release of IP-10, IL-8, TNF-alpha and IL-1beta. Prostaglandins E 122-125 C-X-C motif chemokine ligand 8 Homo sapiens 189-193 18698005-3 2008 In the presence of exogenous PGE(2), peripheral blood mononuclear cells produced higher interleukin-17 (IL-17), C-C chemokine ligand 20 (CCL20)/macrophage inflammatory protein 3alpha (MIP-3alpha), CXC chemokine ligand 8 (CXCL8)/IL-8, and lower interferon-gamma and IL-22 levels than in control cultures. Prostaglandins E 29-32 C-X-C motif chemokine ligand 8 Homo sapiens 197-219 18698005-3 2008 In the presence of exogenous PGE(2), peripheral blood mononuclear cells produced higher interleukin-17 (IL-17), C-C chemokine ligand 20 (CCL20)/macrophage inflammatory protein 3alpha (MIP-3alpha), CXC chemokine ligand 8 (CXCL8)/IL-8, and lower interferon-gamma and IL-22 levels than in control cultures. Prostaglandins E 29-32 C-X-C motif chemokine ligand 8 Homo sapiens 221-226 18698005-3 2008 In the presence of exogenous PGE(2), peripheral blood mononuclear cells produced higher interleukin-17 (IL-17), C-C chemokine ligand 20 (CCL20)/macrophage inflammatory protein 3alpha (MIP-3alpha), CXC chemokine ligand 8 (CXCL8)/IL-8, and lower interferon-gamma and IL-22 levels than in control cultures. Prostaglandins E 29-32 C-X-C motif chemokine ligand 8 Homo sapiens 228-232 18772138-2 2008 We have previously shown that in human T lymphocytes the CHOP phosphorylation induced by prostaglandin E(2) (PGE(2))-increased interleukin-8 (IL-8) gene expression. Prostaglandins E 109-112 C-X-C motif chemokine ligand 8 Homo sapiens 127-140 18772138-2 2008 We have previously shown that in human T lymphocytes the CHOP phosphorylation induced by prostaglandin E(2) (PGE(2))-increased interleukin-8 (IL-8) gene expression. Prostaglandins E 109-112 C-X-C motif chemokine ligand 8 Homo sapiens 142-146 18772138-3 2008 Given the CHOP positive role in the regulation of transcription, here we have investigated the molecular mechanism(s) by which CHOP increases IL-8 gene activity under PGE(2) stimulus. Prostaglandins E 167-170 C-X-C motif chemokine ligand 8 Homo sapiens 142-146 18772138-5 2008 CHOP silencing confirmed its role in the IL-8 transcriptional regulation and protein production, whereas c-Jun small interfering RNA experiments showed that the PGE(2)-induced activation of IL-8 promoter is mainly c-Jun-independent. Prostaglandins E 161-164 C-X-C motif chemokine ligand 8 Homo sapiens 190-194 18772138-6 2008 Moreover, PGE(2) induced CHOP-DNA complexes only when the entire IL-8/AP-1 promoter or the wild type sequences encompassing the AP-1 upstream region were employed. Prostaglandins E 10-13 C-X-C motif chemokine ligand 8 Homo sapiens 65-69 18772138-10 2008 Taken together, our results suggest that the increased expression of CHOP in response to PGE(2) exerts a positive transcriptional regulation of the IL-8 promoter mediated by direct binding to a novel consensus site. Prostaglandins E 89-92 C-X-C motif chemokine ligand 8 Homo sapiens 148-152 20067543-2 2009 Here we demonstrate that tumour-derived prostaglandin E2 (PGE(2)) and transforming growth factor-beta (TGF-beta) increase interleukin-8 (IL-8) but synergistically inhibit interferon-alpha (IFN-alpha) and tumour necrosis factor (TNF) production of Toll-like receptor 7 (TLR7)- and Toll-like receptor 9 (TLR9)-stimulated PDC. Prostaglandins E 58-61 C-X-C motif chemokine ligand 8 Homo sapiens 122-135 20067543-2 2009 Here we demonstrate that tumour-derived prostaglandin E2 (PGE(2)) and transforming growth factor-beta (TGF-beta) increase interleukin-8 (IL-8) but synergistically inhibit interferon-alpha (IFN-alpha) and tumour necrosis factor (TNF) production of Toll-like receptor 7 (TLR7)- and Toll-like receptor 9 (TLR9)-stimulated PDC. Prostaglandins E 58-61 C-X-C motif chemokine ligand 8 Homo sapiens 137-141 19175605-0 2009 Prostaglandin E(2) couples through EP(4) prostanoid receptors to induce IL-8 production in human colonic epithelial cell lines. Prostaglandins E 0-15 C-X-C motif chemokine ligand 8 Homo sapiens 72-76 19175605-8 2009 KEY RESULTS: PGE(2) had the highest affinity for the EP(4) receptor subtype and promoted a robust stimulation of cAMP-dependent IL-8 synthesis. Prostaglandins E 13-16 C-X-C motif chemokine ligand 8 Homo sapiens 128-132 19175605-10 2009 CONCLUSIONS AND IMPLICATIONS: These findings suggest that initiation and progression of colonic inflammation induced by IL-8 could be mediated, at least in part, by PGE(2) acting via the EP(4) receptor subtype. Prostaglandins E 165-168 C-X-C motif chemokine ligand 8 Homo sapiens 120-124 18586957-3 2008 IL-1beta-induced IL-8 release was also repressed by PGE(2) and forskolin, whereas the beta(2)-adrenoceptor agonists were ineffective. Prostaglandins E 52-55 C-X-C motif chemokine ligand 8 Homo sapiens 17-21 17078003-16 2006 These results suggest that the COX-2-PGE(2) pathway may be involved in IL-8 production in gastric epithelial cells. Prostaglandins E 37-40 C-X-C motif chemokine ligand 8 Homo sapiens 71-75 17378789-7 2007 In these cases, the pain score decreased from 6.7 +/- 0.5 (mean +/- standard error of the mean) to 3.3 +/- 0.3 (P < 0.05), and the CSF concentrations of IL-8 and PGE(2) decreased significantly compared with pre-treatment levels (IL-8, 183.3 +/- 21.2 to 116.5 +/- 10.6 pg/ml; PGE(2), 43.8 +/- 10.3 to 14.7 +/- 3.0 pg/ml). Prostaglandins E 165-168 C-X-C motif chemokine ligand 8 Homo sapiens 156-160 17378789-7 2007 In these cases, the pain score decreased from 6.7 +/- 0.5 (mean +/- standard error of the mean) to 3.3 +/- 0.3 (P < 0.05), and the CSF concentrations of IL-8 and PGE(2) decreased significantly compared with pre-treatment levels (IL-8, 183.3 +/- 21.2 to 116.5 +/- 10.6 pg/ml; PGE(2), 43.8 +/- 10.3 to 14.7 +/- 3.0 pg/ml). Prostaglandins E 278-281 C-X-C motif chemokine ligand 8 Homo sapiens 156-160 17078003-2 2006 The effect of PGE(2) on the proinflammatory chemokine interleukin-8 (IL-8) in the gastric epithelial cells has not been defined yet. Prostaglandins E 14-17 C-X-C motif chemokine ligand 8 Homo sapiens 54-67 15659384-2 2005 Here, we demonstrate that, in human T cells, PGE(2) induced IL-8 mRNA transcription through prostaglandin E(2) receptors 1- and 4-dependent signal transduction pathways leading to the activation of the transcription factor C/EBP homologous protein (CHOP), never before implicated in IL-8 transcription. Prostaglandins E 45-48 C-X-C motif chemokine ligand 8 Homo sapiens 60-64 17078003-2 2006 The effect of PGE(2) on the proinflammatory chemokine interleukin-8 (IL-8) in the gastric epithelial cells has not been defined yet. Prostaglandins E 14-17 C-X-C motif chemokine ligand 8 Homo sapiens 69-73 15659384-5 2005 Our data suggest that PGE(2) acts as a potent pro-inflammatory mediator by inducing IL-8 gene transcription in activated T cells through different signal transduction pathways leading to CHOP activation. Prostaglandins E 22-25 C-X-C motif chemokine ligand 8 Homo sapiens 84-88 15659384-2 2005 Here, we demonstrate that, in human T cells, PGE(2) induced IL-8 mRNA transcription through prostaglandin E(2) receptors 1- and 4-dependent signal transduction pathways leading to the activation of the transcription factor C/EBP homologous protein (CHOP), never before implicated in IL-8 transcription. Prostaglandins E 45-48 C-X-C motif chemokine ligand 8 Homo sapiens 283-287 15659384-6 2005 These findings show the complexity with which PGE(2) regulates IL-8 synthesis by inhibiting or enhancing its production depending on the cell types and environmental conditions. Prostaglandins E 46-49 C-X-C motif chemokine ligand 8 Homo sapiens 63-67 15577845-10 2004 PGE(2) , EP2, or EP3 agonists reduced TNF-alpha-induced CCL27 secretion and mRNA levels in parallel to NF-kappaB activity and CCL2, CCL5, CXCL8, and CXCL10 mRNA levels. Prostaglandins E 0-3 C-X-C motif chemokine ligand 8 Homo sapiens 138-143 10623427-12 1999 These results indicate that IL-8 synergizes and LIF potentiates the TNF-alpha PGE(2)effect which appears to be mediated mostly by increasing cPLA2 activity level. Prostaglandins E 78-81 C-X-C motif chemokine ligand 8 Homo sapiens 28-32 12215436-4 2002 PGE(2) (50 nm) attenuated LPS-induced mRNA and protein expression of chemokines including monocyte chemoattractant protein-1, interleukin-8, macrophage inflammatory protein-1alpha and -1beta, and interferon-inducible protein-10. Prostaglandins E 0-3 C-X-C motif chemokine ligand 8 Homo sapiens 126-139 11084285-4 2000 Release of IL-8 was down-regulated by transforming growth factor beta2 (TGF-beta2), by the protein tyrosine-kinase inhibitor genistein, and via rises in intracellular cyclic AMP, generated by prostaglandin E(2), rolipram, pentoxifylline, forskolin, or dibutyryl-cyclic AMP. Prostaglandins E 192-207 C-X-C motif chemokine ligand 8 Homo sapiens 11-15 10893219-4 2000 Maximal IL-8 release was achieved with 10 ng/ml of TGF-beta1 after 16 h of incubation, which was inhibited by the transcription inhibitor actinomycin D and the corticosteroid dexamethasone but was not affected by the nonselective COX inhibitor indomethacin and the selective COX-2 inhibitor NS-398 despite their inhibition on TGF-beta1-induced PGE(2) release. Prostaglandins E 344-347 C-X-C motif chemokine ligand 8 Homo sapiens 8-12 10671814-4 2000 However, the increases in PGE(2) production by IL-6 and IL-8 were found at relatively high concentrations, i.e. at 100 and 200 ng/ml. Prostaglandins E 26-29 C-X-C motif chemokine ligand 8 Homo sapiens 56-60 10433808-3 1999 We have investigated the effects of 5 lipid mediators (PAF, PGE(2), LTB(4), 12-HETE and 15-HETE) on the spontaneous and cytokine-induced IL-8 synthesis by human bone marrow stromal cells. Prostaglandins E 60-63 C-X-C motif chemokine ligand 8 Homo sapiens 137-141 9222021-5 1997 Locally acting prostaglandins modulate vascular permeability, and a synergistic action of prostaglandin E (PGE) with IL-8 has been described. Prostaglandins E 90-105 C-X-C motif chemokine ligand 8 Homo sapiens 117-121 9222021-5 1997 Locally acting prostaglandins modulate vascular permeability, and a synergistic action of prostaglandin E (PGE) with IL-8 has been described. Prostaglandins E 107-110 C-X-C motif chemokine ligand 8 Homo sapiens 117-121 7989497-8 1994 The perivascular location of IL-8 throughout the stages of the human menstrual cycle is consistent with its proposed biological role as a modulator of endometrial function, especially synergism with prostaglandin E and the transmigration of leukocytes. Prostaglandins E 199-214 C-X-C motif chemokine ligand 8 Homo sapiens 29-33 9414445-9 1996 In contrast, the pro-inflammatory action of PGE may be involved in a synergistic action with chemokines such as IL-8 and play a role in parturition. Prostaglandins E 44-47 C-X-C motif chemokine ligand 8 Homo sapiens 112-116 8027281-6 1994 The IL-8 produced in uterine tissues might act synergistically with prostaglandin E (PGE), a likely site for this interaction being blood vessels where PGE production is also repressed by progesterone. Prostaglandins E 68-83 C-X-C motif chemokine ligand 8 Homo sapiens 4-8 8027281-6 1994 The IL-8 produced in uterine tissues might act synergistically with prostaglandin E (PGE), a likely site for this interaction being blood vessels where PGE production is also repressed by progesterone. Prostaglandins E 85-88 C-X-C motif chemokine ligand 8 Homo sapiens 4-8 8027281-6 1994 The IL-8 produced in uterine tissues might act synergistically with prostaglandin E (PGE), a likely site for this interaction being blood vessels where PGE production is also repressed by progesterone. Prostaglandins E 152-155 C-X-C motif chemokine ligand 8 Homo sapiens 4-8 1347804-6 1992 Since prostaglandin E and interleukin-8 have synergistic effects, we suggest that interleukin-8 activity is the final common step of prostaglandin and antiprogestagen action in parturition. Prostaglandins E 6-21 C-X-C motif chemokine ligand 8 Homo sapiens 82-95