PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 11964167-1 2002 1alpha,25-Dihydroxyvitamin D3-mediated transcriptional control of the bone-specific osteocalcin (OC) gene requires the integration of regulatory signals at the vitamin D-responsive element (VDRE) and flanking tissue-specific sequences. Vitamin D 19-28 bone gamma-carboxyglutamate protein Rattus norvegicus 84-95 15211579-3 2004 These treatments caused stable incorporation of p65 into the transcription complex bound to the vitamin D response element (VDRE) of the osteocalcin promoter. Vitamin D 96-105 bone gamma-carboxyglutamate protein Rattus norvegicus 137-148 12746335-6 2003 In tests with other vitamin D responsive elements, such as that from the rat osteocalcin gene, lampVDR showed little or no activity. Vitamin D 20-29 bone gamma-carboxyglutamate protein Rattus norvegicus 77-88 11893738-0 2002 Histone acetylation in vivo at the osteocalcin locus is functionally linked to vitamin D-dependent, bone tissue-specific transcription. Vitamin D 79-88 bone gamma-carboxyglutamate protein Rattus norvegicus 35-46 11893738-6 2002 Acetylation of H4 at the OC gene is selectively increased following vitamin D(3) enhancement of OC transcription, with the most prominent changes occurring in the region between the vitamin D(3) enhancer and basal promoter. Vitamin D 68-77 bone gamma-carboxyglutamate protein Rattus norvegicus 25-27 11893738-6 2002 Acetylation of H4 at the OC gene is selectively increased following vitamin D(3) enhancement of OC transcription, with the most prominent changes occurring in the region between the vitamin D(3) enhancer and basal promoter. Vitamin D 68-77 bone gamma-carboxyglutamate protein Rattus norvegicus 96-98 11893738-6 2002 Acetylation of H4 at the OC gene is selectively increased following vitamin D(3) enhancement of OC transcription, with the most prominent changes occurring in the region between the vitamin D(3) enhancer and basal promoter. Vitamin D 182-191 bone gamma-carboxyglutamate protein Rattus norvegicus 25-27 11893738-7 2002 Thus, our results suggest functional linkage of H3 and H4 acetylation in specific regions of the OC promoter to chromatin remodeling that accompanies tissue-specific transcriptional activation and vitamin D enhancement of OC gene expression. Vitamin D 197-206 bone gamma-carboxyglutamate protein Rattus norvegicus 97-99 11893738-7 2002 Thus, our results suggest functional linkage of H3 and H4 acetylation in specific regions of the OC promoter to chromatin remodeling that accompanies tissue-specific transcriptional activation and vitamin D enhancement of OC gene expression. Vitamin D 197-206 bone gamma-carboxyglutamate protein Rattus norvegicus 222-224 12960019-2 2003 To further define the residues in the vitamin D receptor (VDR) DNA binding domain (DBD) that mediate its interaction as a retinoid X receptor (RXR) heterodimer with the rat osteocalcin vitamin D-responsive element (VDRE), chimeric receptors were created in which the core DBD of VDR was replaced with that of the homodimerizing glucocorticoid receptor (GR). Vitamin D 38-47 bone gamma-carboxyglutamate protein Rattus norvegicus 173-184 11964167-1 2002 1alpha,25-Dihydroxyvitamin D3-mediated transcriptional control of the bone-specific osteocalcin (OC) gene requires the integration of regulatory signals at the vitamin D-responsive element (VDRE) and flanking tissue-specific sequences. Vitamin D 19-28 bone gamma-carboxyglutamate protein Rattus norvegicus 97-99 11179723-0 2001 Contributions of nuclear architecture and chromatin to vitamin D-dependent transcriptional control of the rat osteocalcin gene. Vitamin D 55-64 bone gamma-carboxyglutamate protein Rattus norvegicus 110-121 11891855-2 2002 In osseous cells expressing OC, the promoter region contains two nuclease hypersensitive sites that encompass the elements that regulate basal tissue-specific and vitamin D-enhanced OC transcription. Vitamin D 163-172 bone gamma-carboxyglutamate protein Rattus norvegicus 28-30 11891855-2 2002 In osseous cells expressing OC, the promoter region contains two nuclease hypersensitive sites that encompass the elements that regulate basal tissue-specific and vitamin D-enhanced OC transcription. Vitamin D 163-172 bone gamma-carboxyglutamate protein Rattus norvegicus 182-184 11668178-4 2002 Expression of both C/EBPbeta and C/EBPdelta increases from the growth to maturation developmental stages and, like the bone-specific osteocalcin (OC) gene, is also stimulated 3-6-fold by vitamin D(3), a regulator of osteoblast differentiation. Vitamin D 187-196 bone gamma-carboxyglutamate protein Rattus norvegicus 133-144 11179723-1 2001 The vitamin D response element in the bone tissue-specific osteocalcin gene has served as a prototype for understanding molecular mechanisms regulating physiologic responsiveness of vitamin D-dependent genes in bone cells. Vitamin D 4-13 bone gamma-carboxyglutamate protein Rattus norvegicus 59-70 11179723-1 2001 The vitamin D response element in the bone tissue-specific osteocalcin gene has served as a prototype for understanding molecular mechanisms regulating physiologic responsiveness of vitamin D-dependent genes in bone cells. Vitamin D 182-191 bone gamma-carboxyglutamate protein Rattus norvegicus 59-70 11179723-4 2001 We present evidence for molecular mechanisms regulating vitamin D-dependent mediated transcription of the osteocalcin gene that involve chromatin structure of the gene and nuclear architecture. Vitamin D 56-65 bone gamma-carboxyglutamate protein Rattus norvegicus 106-117 10523637-0 1999 Multiple Cbfa/AML sites in the rat osteocalcin promoter are required for basal and vitamin D-responsive transcription and contribute to chromatin organization. Vitamin D 83-92 bone gamma-carboxyglutamate protein Rattus norvegicus 35-46 10896790-2 2000 Recent studies have reported diametrically opposing responses in the vitamin D regulation of the mouse vs the human and rat osteocalcin genes. Vitamin D 69-78 bone gamma-carboxyglutamate protein Rattus norvegicus 124-135 8923469-0 1996 Distinct conformations of vitamin D receptor/retinoid X receptor-alpha heterodimers are specified by dinucleotide differences in the vitamin D-responsive elements of the osteocalcin and osteopontin genes. Vitamin D 26-35 bone gamma-carboxyglutamate protein Rattus norvegicus 170-181 10321839-1 1999 The sequences in the rat osteocalcin gene that lie 3" to the vitamin D response element (VDRE) contain a GGTTTGG motif (-420 to -414) that is essential for transcriptional activation of osteocalcin-CAT (OC-CAT) fusion genes by 1,25(OH)2D3. Vitamin D 61-70 bone gamma-carboxyglutamate protein Rattus norvegicus 25-36 10321839-1 1999 The sequences in the rat osteocalcin gene that lie 3" to the vitamin D response element (VDRE) contain a GGTTTGG motif (-420 to -414) that is essential for transcriptional activation of osteocalcin-CAT (OC-CAT) fusion genes by 1,25(OH)2D3. Vitamin D 61-70 bone gamma-carboxyglutamate protein Rattus norvegicus 186-197 9886808-0 1999 AP-1 and vitamin D receptor (VDR) signaling pathways converge at the rat osteocalcin VDR element: requirement for the internal activating protein-1 site for vitamin D-mediated trans-activation. Vitamin D 9-18 bone gamma-carboxyglutamate protein Rattus norvegicus 73-84 9886808-3 1999 The hormonal form of vitamin D, 1,25-dihydroxyvitamin D3, stimulates transcription of the tissue-specific osteocalcin (OC) gene in osteoblastic cells. Vitamin D 21-30 bone gamma-carboxyglutamate protein Rattus norvegicus 106-117 9886808-3 1999 The hormonal form of vitamin D, 1,25-dihydroxyvitamin D3, stimulates transcription of the tissue-specific osteocalcin (OC) gene in osteoblastic cells. Vitamin D 21-30 bone gamma-carboxyglutamate protein Rattus norvegicus 119-121 9886808-4 1999 The rat OC vitamin D response element contains an internal acitvating protein-1 (AP-1) site. Vitamin D 11-20 bone gamma-carboxyglutamate protein Rattus norvegicus 8-10 9886808-5 1999 Here, we report for the first time that this AP-1 site is critical for the transcriptional enhancement of rat osteocalcin gene expression mediated by vitamin D. Vitamin D 150-159 bone gamma-carboxyglutamate protein Rattus norvegicus 110-121 9886808-8 1999 These results suggest a functional interaction between the hormone receptor and nuclear oncoproteins at the rat OC vitamin D response element. Vitamin D 115-124 bone gamma-carboxyglutamate protein Rattus norvegicus 112-114 9010346-10 1996 In addition, the genomic action of each vitamin D compound was assessed in a rat osteosarcoma cell line (ROS 17/2.8) by measuring its ability to transactivate a gene construct containing the vitamin D response element of the osteocalcin gene linked to the growth hormone reporter gene. Vitamin D 40-49 bone gamma-carboxyglutamate protein Rattus norvegicus 225-236 9010346-10 1996 In addition, the genomic action of each vitamin D compound was assessed in a rat osteosarcoma cell line (ROS 17/2.8) by measuring its ability to transactivate a gene construct containing the vitamin D response element of the osteocalcin gene linked to the growth hormone reporter gene. Vitamin D 191-200 bone gamma-carboxyglutamate protein Rattus norvegicus 225-236 10022617-5 1999 Indeed, the absence of hypersensitivity was accompanied by inhibition of basal and vitamin D-dependent enhancement of OC gene transcription. Vitamin D 83-92 bone gamma-carboxyglutamate protein Rattus norvegicus 118-120 9548563-0 1998 Multiple levels of steroid hormone-dependent control of osteocalcin during osteoblast differentiation: glucocorticoid regulation of basal and vitamin D stimulated gene expression. Vitamin D 142-151 bone gamma-carboxyglutamate protein Rattus norvegicus 56-67 9548563-1 1998 We have examined the contribution of transcriptional mechanisms to the pleiotropic effects of glucocorticoids on basal and vitamin D stimulated expression of the developmentally regulated bone-specific osteocalcin (OC) gene. Vitamin D 123-132 bone gamma-carboxyglutamate protein Rattus norvegicus 202-213 9548563-1 1998 We have examined the contribution of transcriptional mechanisms to the pleiotropic effects of glucocorticoids on basal and vitamin D stimulated expression of the developmentally regulated bone-specific osteocalcin (OC) gene. Vitamin D 123-132 bone gamma-carboxyglutamate protein Rattus norvegicus 215-217 9013768-0 1997 DNA sequences downstream from the vitamin D response element of the rat osteocalcin gene are required for ligand-dependent transactivation. Vitamin D 34-43 bone gamma-carboxyglutamate protein Rattus norvegicus 72-83 9013768-1 1997 The sequences in the rat osteocalcin gene that lie 3" to the vitamin D response element (VDRE) have been shown to augment transcriptional activation by 1,25-dihydroxyvitamin D3 [1,25-(OH)2D3]. Vitamin D 61-70 bone gamma-carboxyglutamate protein Rattus norvegicus 25-36 7588324-5 1995 Vitamin D-induced transcription was assayed in transfected ROS 17/2.8 osteosarcoma cells using chloraminphenicol acetyltransferase constructs containing the vitamin D-responsive element (VDRE) at its native locus in the rat OC promoter as well as fused to a heterologous promoter. Vitamin D 0-9 bone gamma-carboxyglutamate protein Rattus norvegicus 224-226 8603577-0 1996 Basal and vitamin D-responsive activity of the rat osteocalcin promoter in stably transfected osteosarcoma cells: requirement of upstream sequences for control by the proximal regulatory domain. Vitamin D 10-19 bone gamma-carboxyglutamate protein Rattus norvegicus 51-62 8603577-1 1996 Osteocalcin (OC) is a bone-specific vitamin D- responsive protein that is developmentally expressed during osteoblast differentiation. Vitamin D 36-45 bone gamma-carboxyglutamate protein Rattus norvegicus 0-11 8603577-1 1996 Osteocalcin (OC) is a bone-specific vitamin D- responsive protein that is developmentally expressed during osteoblast differentiation. Vitamin D 36-45 bone gamma-carboxyglutamate protein Rattus norvegicus 13-15 8603577-11 1996 Cells carrying the 1.1-kb promoter show DNase I hypersensitivity at both the basal promoter and the vitamin D response element-containing domains, locations that also exhibit DNase I hypersensitivity in the endogenous OC promoter. Vitamin D 100-109 bone gamma-carboxyglutamate protein Rattus norvegicus 218-220 8900396-1 1996 The binding of the 1,25-dihydroxyvitamin D3 receptor to the vitamin D response elements (VDREs) in the rat osteocalcin (OSC-DRE), mouse osteopontin (MOP-DRE), rat calbindin D-9k (CaBP-DRE), and human parathyroid hormone genes (PTH-DRE) was studied. Vitamin D 33-42 bone gamma-carboxyglutamate protein Rattus norvegicus 107-118 7588324-6 1995 Both ST and OA inhibit VDRE-mediated and vitamin D-dependent enhancement of OC gene transcription as well as OC biosynthesis, as assessed by RIAs. Vitamin D 41-50 bone gamma-carboxyglutamate protein Rattus norvegicus 76-78 7598807-4 1995 As well as containing a vitamin D response element, the upstream region of the osteocalcin promoter also has potent basal activity in the osteoblast-like ROS17/2.8 cell line. Vitamin D 24-33 bone gamma-carboxyglutamate protein Rattus norvegicus 79-90 7640305-1 1995 The effects of two vitamin D analogs, 1,25-dihydroxyvitamin D-2 and 24-epi-1,25-dihydroxyvitamin D-2, were examined on osteocalcin gene expression in the rat osteosarcoma cell line ROS 17/28. Vitamin D 19-28 bone gamma-carboxyglutamate protein Rattus norvegicus 119-130 7626514-4 1995 The expressed hVDR displays strict dependence on the family of retinoid X receptors (RXRs) for binding to the vitamin D-responsive element (VDRE) in the rat osteocalcin gene. Vitamin D 110-119 bone gamma-carboxyglutamate protein Rattus norvegicus 157-168 8286356-0 1994 DNase I hypersensitive sites in promoter elements associated with basal and vitamin D dependent transcription of the bone-specific osteocalcin gene. Vitamin D 76-85 bone gamma-carboxyglutamate protein Rattus norvegicus 131-142 7708726-3 1995 Most important, the two specific complexes formed by porcine nuclear extract with the vitamin D response elements from either the osteocalcin gene or the rat 24-hydroxylase gene are shifted to a larger complex by both an anti-vitamin D receptor antibody and an anti-RXR antibody, leaving no doubt that in vivo the nuclear accessory factor for the vitamin D receptor in the intestine is an RXR protein. Vitamin D 86-95 bone gamma-carboxyglutamate protein Rattus norvegicus 130-141 7813631-9 1995 Although differentiation-related genes (alkaline phosphatase and osteocalcin) were up-regulated by vitamin D, culture on the collagen matrix could not overcome the inhibition of mineralization. Vitamin D 99-108 bone gamma-carboxyglutamate protein Rattus norvegicus 65-76 7809144-0 1994 In vivo occupancy of the vitamin D responsive element in the osteocalcin gene supports vitamin D-dependent transcriptional upregulation in intact cells. Vitamin D 25-34 bone gamma-carboxyglutamate protein Rattus norvegicus 61-72 7809144-0 1994 In vivo occupancy of the vitamin D responsive element in the osteocalcin gene supports vitamin D-dependent transcriptional upregulation in intact cells. Vitamin D 87-96 bone gamma-carboxyglutamate protein Rattus norvegicus 61-72 7809144-6 1994 Our results establish in intact cells the requirement for both ligand- and receptor-dependent occupancy of the VDRE for vitamin D responsive enhancement of osteocalcin gene transcription. Vitamin D 120-129 bone gamma-carboxyglutamate protein Rattus norvegicus 156-167 8194478-0 1994 Inhibition of 1,25-dihydroxyvitamin D3 stimulated osteocalcin gene transcription by tumor necrosis factor-alpha: structural determinants within the vitamin D response element. Vitamin D 28-37 bone gamma-carboxyglutamate protein Rattus norvegicus 50-61 8179318-2 1994 The purified receptor is homogeneous, and is bound by 1,25-dihydroxyvitamin D3 with a Kd of 5 x 10(-10) M. The isolated receptor binds to the osteocalcin vitamin D response element in the presence of porcine intestinal nuclear extract stripped of endogenous vitamin D receptor as well. Vitamin D 68-77 bone gamma-carboxyglutamate protein Rattus norvegicus 142-153 8014199-6 1994 NMP-2 exhibits recognition for a promoter domain contiguous to the vitamin D-responsive element of the osteocalcin gene, although the vitamin D receptor does not appear to be a component of the nuclear matrix proteins. Vitamin D 67-76 bone gamma-carboxyglutamate protein Rattus norvegicus 103-114 8286356-4 1994 Together, these elements regulate basal and vitamin D enhanced osteocalcin gene transcription. Vitamin D 44-53 bone gamma-carboxyglutamate protein Rattus norvegicus 63-74 8286356-6 1994 Both in confluent cultures and in response to vitamin D, when osteocalcin transcription was upregulated, the hypersensitive bands were significantly intensified. Vitamin D 46-55 bone gamma-carboxyglutamate protein Rattus norvegicus 62-73 8218210-3 1993 In this study, we established that the -1097 to +23 promoter (pOCZCat) of the rat OC gene confers glucocorticoid responsiveness to both basal and vitamin D-induced OC expression. Vitamin D 146-155 bone gamma-carboxyglutamate protein Rattus norvegicus 63-65 8243336-1 1993 The osteocalcin (OC) gene was initially described as a single copy gene encoding the bone specific vitamin K dependent and vitamin D regulated protein. Vitamin D 123-132 bone gamma-carboxyglutamate protein Rattus norvegicus 4-15 8243336-1 1993 The osteocalcin (OC) gene was initially described as a single copy gene encoding the bone specific vitamin K dependent and vitamin D regulated protein. Vitamin D 123-132 bone gamma-carboxyglutamate protein Rattus norvegicus 17-19 8218210-3 1993 In this study, we established that the -1097 to +23 promoter (pOCZCat) of the rat OC gene confers glucocorticoid responsiveness to both basal and vitamin D-induced OC expression. Vitamin D 146-155 bone gamma-carboxyglutamate protein Rattus norvegicus 82-84 8218210-10 1993 The presence of multiple GR binding sites in the rat OC gene proximal promoter indicates that regulation of basal and vitamin D-enhanced transcription by glucocorticoids may involve the integrated activities of multiple, independent GREs. Vitamin D 118-127 bone gamma-carboxyglutamate protein Rattus norvegicus 53-55 8381969-0 1993 Postproliferative transcription of the rat osteocalcin gene is reflected by vitamin D-responsive developmental modifications in protein-DNA interactions at basal and enhancer promoter elements. Vitamin D 76-85 bone gamma-carboxyglutamate protein Rattus norvegicus 43-54 8394128-6 1993 Transient transfection of osteosarcoma cells with a reporter vector containing a vitamin D responsive element derived from the rat osteocalcin gene yields equivalent transcriptional activation in the presence of either 1,25(OH)2D3 or OCT. Further experiments performed at various 1,25(OH)2D3 concentrations to assess the relationship between receptor phosphorylation and transcriptional activity in intact cells showed a positive correlation between these two parameters, indicating that the 1,25(OH)2D3 hormone stimulates VDR phosphorylation and transcriptional activation in parallel. Vitamin D 81-90 bone gamma-carboxyglutamate protein Rattus norvegicus 131-142 8381969-7 1993 For example, in proliferating osteoblasts, a vitamin D receptor-antibody-sensitive complex is formed that is different from the DNA binding complex induced by vitamin D postproliferatively when the OC gene is transcribed. Vitamin D 45-54 bone gamma-carboxyglutamate protein Rattus norvegicus 198-200 8377726-6 1993 We conclude that PTHrP(1-34) causes similar effects on bone, in organ cultures, to those caused by bPTH(1-34), namely an increase in both bone resorption and formation and a decrease in the vitamin D-stimulated BGP synthesis. Vitamin D 190-199 bone gamma-carboxyglutamate protein Rattus norvegicus 211-214 8380156-9 1993 When tested in a COS-1 cell transfection assay system using a rat osteocalcin vitamin D responsive element coupled to a growth hormone reporter gene, 1 alpha,25-(OH)2DHT3 showed a biological activity only 10 times lower than 1 alpha,25-(OH)2D3. Vitamin D 78-87 bone gamma-carboxyglutamate protein Rattus norvegicus 66-77 1864858-0 1991 The cellular and extracellular distribution of osteocalcin and dentin phosphoprotein in teeth of vitamin D-deficient rats. Vitamin D 97-106 bone gamma-carboxyglutamate protein Rattus norvegicus 47-58 1472030-7 1992 Our results represent the first demonstration of the effect of active vitamin D and corticosteroid on the expression of osteocalcin mRNA in bone in vivo. Vitamin D 70-79 bone gamma-carboxyglutamate protein Rattus norvegicus 120-131 1321435-0 1992 Vitamin D-responsive protein-DNA interactions at multiple promoter regulatory elements that contribute to the level of rat osteocalcin gene expression. Vitamin D 0-9 bone gamma-carboxyglutamate protein Rattus norvegicus 123-134 1321435-1 1992 The observation that vitamin D-mediated enhancement of osteocalcin (OC) gene expression is dependent on and reciprocally related to the level of basal gene expression suggests that an interaction of the vitamin D responsive element (VDRE) with basal regulatory elements of the OC gene promoter contributes to both basal and vitamin D-enhanced transcription. Vitamin D 21-30 bone gamma-carboxyglutamate protein Rattus norvegicus 55-66 1321435-1 1992 The observation that vitamin D-mediated enhancement of osteocalcin (OC) gene expression is dependent on and reciprocally related to the level of basal gene expression suggests that an interaction of the vitamin D responsive element (VDRE) with basal regulatory elements of the OC gene promoter contributes to both basal and vitamin D-enhanced transcription. Vitamin D 21-30 bone gamma-carboxyglutamate protein Rattus norvegicus 68-70 1321435-1 1992 The observation that vitamin D-mediated enhancement of osteocalcin (OC) gene expression is dependent on and reciprocally related to the level of basal gene expression suggests that an interaction of the vitamin D responsive element (VDRE) with basal regulatory elements of the OC gene promoter contributes to both basal and vitamin D-enhanced transcription. Vitamin D 21-30 bone gamma-carboxyglutamate protein Rattus norvegicus 277-279 1321435-1 1992 The observation that vitamin D-mediated enhancement of osteocalcin (OC) gene expression is dependent on and reciprocally related to the level of basal gene expression suggests that an interaction of the vitamin D responsive element (VDRE) with basal regulatory elements of the OC gene promoter contributes to both basal and vitamin D-enhanced transcription. Vitamin D 203-212 bone gamma-carboxyglutamate protein Rattus norvegicus 55-66 1321435-1 1992 The observation that vitamin D-mediated enhancement of osteocalcin (OC) gene expression is dependent on and reciprocally related to the level of basal gene expression suggests that an interaction of the vitamin D responsive element (VDRE) with basal regulatory elements of the OC gene promoter contributes to both basal and vitamin D-enhanced transcription. Vitamin D 203-212 bone gamma-carboxyglutamate protein Rattus norvegicus 68-70 1321435-1 1992 The observation that vitamin D-mediated enhancement of osteocalcin (OC) gene expression is dependent on and reciprocally related to the level of basal gene expression suggests that an interaction of the vitamin D responsive element (VDRE) with basal regulatory elements of the OC gene promoter contributes to both basal and vitamin D-enhanced transcription. Vitamin D 203-212 bone gamma-carboxyglutamate protein Rattus norvegicus 277-279 1321435-1 1992 The observation that vitamin D-mediated enhancement of osteocalcin (OC) gene expression is dependent on and reciprocally related to the level of basal gene expression suggests that an interaction of the vitamin D responsive element (VDRE) with basal regulatory elements of the OC gene promoter contributes to both basal and vitamin D-enhanced transcription. Vitamin D 203-212 bone gamma-carboxyglutamate protein Rattus norvegicus 55-66 1321435-1 1992 The observation that vitamin D-mediated enhancement of osteocalcin (OC) gene expression is dependent on and reciprocally related to the level of basal gene expression suggests that an interaction of the vitamin D responsive element (VDRE) with basal regulatory elements of the OC gene promoter contributes to both basal and vitamin D-enhanced transcription. Vitamin D 203-212 bone gamma-carboxyglutamate protein Rattus norvegicus 68-70 1321435-1 1992 The observation that vitamin D-mediated enhancement of osteocalcin (OC) gene expression is dependent on and reciprocally related to the level of basal gene expression suggests that an interaction of the vitamin D responsive element (VDRE) with basal regulatory elements of the OC gene promoter contributes to both basal and vitamin D-enhanced transcription. Vitamin D 203-212 bone gamma-carboxyglutamate protein Rattus norvegicus 277-279 1321435-3 1992 In addition, a vitamin D-responsive increase in OC gene transcription is accompanied by enhanced non-vitamin D receptor-mediated protein-DNA interactions in the "TATA" box region (nucleotides -44 to +23), which also contains a potential glucocorticoid responsive element. Vitamin D 15-24 bone gamma-carboxyglutamate protein Rattus norvegicus 48-50 1321435-7 1992 A model is presented for the contribution of both the VDRE and proximal promoter elements to the enhancement of OC gene transcription in response to vitamin D. Vitamin D 149-158 bone gamma-carboxyglutamate protein Rattus norvegicus 112-114 1466160-4 1992 The concentrations of two vitamin D-dependent calcium-binding proteins were also decreased: a low duodenal calbindin-D 9K concentration corresponding to the low intestinal active calcium absorption and a low serum osteocalcin concentration, corresponding to a low bone formation and highly correlated with serum IGF-I concentration. Vitamin D 26-35 bone gamma-carboxyglutamate protein Rattus norvegicus 214-225 1309841-7 1992 After transient transfection of PHOC-CAT into ROS 17/2.8 osteosarcoma cells, reporter CAT activity was up-regulated by vitamin D at concentrations above 10(-12) M. In screening studies, TNF-alpha (-57%) and IL-6 (-37%) inhibited vitamin D-stimulated osteocalcin transcription, whereas IL-1 alpha, IL-1 beta, and IL-8 had no effect. Vitamin D 119-128 bone gamma-carboxyglutamate protein Rattus norvegicus 250-261 1757481-0 1991 Influence of dexamethasone on the vitamin D-mediated regulation of osteocalcin gene expression. Vitamin D 34-43 bone gamma-carboxyglutamate protein Rattus norvegicus 67-78 1757481-3 1991 However, dexamethasone significantly inhibits these parameters of the vitamin D-induced upregulation of osteocalcin gene expression in both proliferating and in confluent ROS 17/2.8 cells. Vitamin D 70-79 bone gamma-carboxyglutamate protein Rattus norvegicus 104-115 1757481-4 1991 In this study, we observed that the extent to which abrogation of the vitamin D response occurs is dependent on basal levels of osteocalcin gene expression as reflected by a complete inhibition of the vitamin D-induced upregulation in a ROS 17/2.8K subline with low basal expression and only a partial reduction of the vitamin D stimulation in a ROS 17/2.8C subline with eightfold higher levels of basal expression. Vitamin D 70-79 bone gamma-carboxyglutamate protein Rattus norvegicus 128-139 1757481-4 1991 In this study, we observed that the extent to which abrogation of the vitamin D response occurs is dependent on basal levels of osteocalcin gene expression as reflected by a complete inhibition of the vitamin D-induced upregulation in a ROS 17/2.8K subline with low basal expression and only a partial reduction of the vitamin D stimulation in a ROS 17/2.8C subline with eightfold higher levels of basal expression. Vitamin D 201-210 bone gamma-carboxyglutamate protein Rattus norvegicus 128-139 1757481-4 1991 In this study, we observed that the extent to which abrogation of the vitamin D response occurs is dependent on basal levels of osteocalcin gene expression as reflected by a complete inhibition of the vitamin D-induced upregulation in a ROS 17/2.8K subline with low basal expression and only a partial reduction of the vitamin D stimulation in a ROS 17/2.8C subline with eightfold higher levels of basal expression. Vitamin D 201-210 bone gamma-carboxyglutamate protein Rattus norvegicus 128-139 1757481-6 1991 The complexity of the glucocorticoid effect on vitamin D-mediated transcriptional properties of the osteocalcin gene is indicated by persistence of sequence-specific protein-DNA interactions at two principal osteocalcin gene promoter regulatory elements, the osteocalcin (CCAAT) box which modulates basal level of transcription, and the vitamin D responsive element, where vitamin D-mediated enhancement of osteocalcin gene transcription is controlled. Vitamin D 47-56 bone gamma-carboxyglutamate protein Rattus norvegicus 100-111 1757481-6 1991 The complexity of the glucocorticoid effect on vitamin D-mediated transcriptional properties of the osteocalcin gene is indicated by persistence of sequence-specific protein-DNA interactions at two principal osteocalcin gene promoter regulatory elements, the osteocalcin (CCAAT) box which modulates basal level of transcription, and the vitamin D responsive element, where vitamin D-mediated enhancement of osteocalcin gene transcription is controlled. Vitamin D 47-56 bone gamma-carboxyglutamate protein Rattus norvegicus 208-219 1757481-6 1991 The complexity of the glucocorticoid effect on vitamin D-mediated transcriptional properties of the osteocalcin gene is indicated by persistence of sequence-specific protein-DNA interactions at two principal osteocalcin gene promoter regulatory elements, the osteocalcin (CCAAT) box which modulates basal level of transcription, and the vitamin D responsive element, where vitamin D-mediated enhancement of osteocalcin gene transcription is controlled. Vitamin D 47-56 bone gamma-carboxyglutamate protein Rattus norvegicus 208-219 1757481-6 1991 The complexity of the glucocorticoid effect on vitamin D-mediated transcriptional properties of the osteocalcin gene is indicated by persistence of sequence-specific protein-DNA interactions at two principal osteocalcin gene promoter regulatory elements, the osteocalcin (CCAAT) box which modulates basal level of transcription, and the vitamin D responsive element, where vitamin D-mediated enhancement of osteocalcin gene transcription is controlled. Vitamin D 47-56 bone gamma-carboxyglutamate protein Rattus norvegicus 208-219 1757481-6 1991 The complexity of the glucocorticoid effect on vitamin D-mediated transcriptional properties of the osteocalcin gene is indicated by persistence of sequence-specific protein-DNA interactions at two principal osteocalcin gene promoter regulatory elements, the osteocalcin (CCAAT) box which modulates basal level of transcription, and the vitamin D responsive element, where vitamin D-mediated enhancement of osteocalcin gene transcription is controlled. Vitamin D 337-346 bone gamma-carboxyglutamate protein Rattus norvegicus 100-111 1757481-6 1991 The complexity of the glucocorticoid effect on vitamin D-mediated transcriptional properties of the osteocalcin gene is indicated by persistence of sequence-specific protein-DNA interactions at two principal osteocalcin gene promoter regulatory elements, the osteocalcin (CCAAT) box which modulates basal level of transcription, and the vitamin D responsive element, where vitamin D-mediated enhancement of osteocalcin gene transcription is controlled. Vitamin D 337-346 bone gamma-carboxyglutamate protein Rattus norvegicus 100-111 1864858-11 1991 These data suggest that vitamin D acts directly on odontogenic cells at various synthetic (osteocalcin) or secretory (phosphoprotein) levels indicating that odontoblasts are target-cells for vitamin D. Vitamin D 24-33 bone gamma-carboxyglutamate protein Rattus norvegicus 91-102 2784002-0 1989 Structure of the rat osteocalcin gene and regulation of vitamin D-dependent expression. Vitamin D 56-65 bone gamma-carboxyglutamate protein Rattus norvegicus 21-32 1653893-0 1991 The vitamin D-responsive element in the rat bone Gla protein gene is an imperfect direct repeat that cooperates with other cis-elements in 1,25-dihydroxyvitamin D3- mediated transcriptional activation. Vitamin D 4-13 bone gamma-carboxyglutamate protein Rattus norvegicus 44-60 2101404-0 1990 Osteocalcin is vitamin D-dependent during the perinatal period in the rat. Vitamin D 15-24 bone gamma-carboxyglutamate protein Rattus norvegicus 0-11 2101404-1 1990 The vitamin D-dependence of renal calbindin D-28K and osteocalcin during the perinatal period was studied in fetuses (days 18 and 21) and neonates (days 2, 12, 17 and 22) of rats fed either a standard diet (0.85% Ca-0.7% P; "high Ca-P diet" rats) or a mildly Ca-P restricted diet (0.2% Ca-0.2% P; "low Ca-P diet" rats). Vitamin D 4-13 bone gamma-carboxyglutamate protein Rattus norvegicus 54-65 2101404-8 1990 These studies indicate that osteocalcin is vitamin D-dependent in the fetal and neonatal rat. Vitamin D 43-52 bone gamma-carboxyglutamate protein Rattus norvegicus 28-39 2308930-0 1990 Vitamin D-mediated modifications in protein-DNA interactions at two promoter elements of the osteocalcin gene. Vitamin D 0-9 bone gamma-carboxyglutamate protein Rattus norvegicus 93-104 2308930-5 1990 We have also observed that vitamin D stimulation of osteocalcin gene expression results in a 5-fold increase in protein binding to the region of the osteocalcin box, a 24-nucleotide segment in the proximal promoter with a CCAAT motif as the central core. Vitamin D 27-36 bone gamma-carboxyglutamate protein Rattus norvegicus 52-63 2308930-5 1990 We have also observed that vitamin D stimulation of osteocalcin gene expression results in a 5-fold increase in protein binding to the region of the osteocalcin box, a 24-nucleotide segment in the proximal promoter with a CCAAT motif as the central core. Vitamin D 27-36 bone gamma-carboxyglutamate protein Rattus norvegicus 149-160 2308930-6 1990 Our results demonstrate protein-DNA interactions in a vitamin D-responsive element and in a second sequence, the osteocalcin box, both of which are involved in the physiologic regulation of the osteocalcin gene in response to 1,25-dihydroxyvitamin D3. Vitamin D 54-63 bone gamma-carboxyglutamate protein Rattus norvegicus 194-205 3105848-1 1987 Vitamin D-deficient, second generation, rachitic rats showed significant decrease in bone Gla protein (BGP) levels in circulation and in the skeleton. Vitamin D 0-9 bone gamma-carboxyglutamate protein Rattus norvegicus 85-101 2605954-6 1989 First, contained within the 600 nucleotides immediately upstream from the transcription initiation site are sequences which support Vitamin D dependent transcription of the rat osteocalcin gene. Vitamin D 132-141 bone gamma-carboxyglutamate protein Rattus norvegicus 177-188 3494594-1 1987 Osteocalcin, the vitamin K-dependent protein in bone containing gamma-carboxyglutamic acid, has been found to be significantly decreased in the osteomalacic bone of chicks made vitamin D deficient for 6 weeks. Vitamin D 177-186 bone gamma-carboxyglutamate protein Rattus norvegicus 0-11 3494594-5 1987 In the vitamin D-deficient animals, osteomalacia was evident histologically by 7 weeks, at which time serum 1,25-(OH)2D3 was not detectable, bone osteocalcin was decreased by 50%, and serum osteocalcin was decreased by 20%. Vitamin D 7-16 bone gamma-carboxyglutamate protein Rattus norvegicus 146-157 3494594-5 1987 In the vitamin D-deficient animals, osteomalacia was evident histologically by 7 weeks, at which time serum 1,25-(OH)2D3 was not detectable, bone osteocalcin was decreased by 50%, and serum osteocalcin was decreased by 20%. Vitamin D 7-16 bone gamma-carboxyglutamate protein Rattus norvegicus 190-201 3494594-7 1987 These data are consistent with the conclusion that the metabolism of osteocalcin is affected by serum 1,25-(OH)2D3 and that the diminished level of osteocalcin in the bone of vitamin D-deficient animals is the result of a direct action of the metabolites and is not secondary to a decrease in the mineralization of bone tissue. Vitamin D 175-184 bone gamma-carboxyglutamate protein Rattus norvegicus 148-159 3105848-1 1987 Vitamin D-deficient, second generation, rachitic rats showed significant decrease in bone Gla protein (BGP) levels in circulation and in the skeleton. Vitamin D 0-9 bone gamma-carboxyglutamate protein Rattus norvegicus 103-106