PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 2868714-3 1986 The properties of the technique are illustrated by an investigation of cleavage reactions of the disulfide bonds in bovine insulin, cyclic somatostatin, and conotoxin G1 utilizing the reducing agent dithiothreitol. Disulfides 97-106 insulin Bos taurus 123-169 3180835-0 1988 The occurrence of glutathione-insulin transhydrogenase (protein-disulfide interchange enzyme) in the lens. Disulfides 64-73 insulin Bos taurus 30-37 3899711-0 1985 Nonspecific reaction of a thiol: protein disulfide oxidoreductase with the disulfide bonds of insulin. Disulfides 41-50 insulin Bos taurus 94-101 681081-1 1978 An insulin fragment containing residues A 18-21 and B 19-26 linked by the disulfide bond between residues A 20 and B 19 was synthesized. Disulfides 74-83 insulin Bos taurus 3-10 28194735-6 2017 Since disulfide bond can be selectively cleaved and modified upon methoxy addition, subsequent MS2 CID of the methoxy addition product provides enhanced sequence coverage as demonstrated by the analysis of bovine insulin. Disulfides 6-15 insulin Bos taurus 213-220 29142703-0 2015 Mechanisms and energetics of free radical initiated disulfide bond cleavage in model peptides and insulin by mass spectrometry. Disulfides 52-61 insulin Bos taurus 98-105 29997815-2 2016 The site-selective functionalization of (a) the peptide hormone somatostatin, (b) the interchain disulfide of bovine insulin and (c) functionalization of the proteins GFP and lysozyme with allyl sulfones proceeds in aqueous solution. Disulfides 97-106 insulin Bos taurus 117-124 29142703-3 2015 We show that even multiple disulfide bonds in intact bovine insulin are fragmented in the MS2 stage, releasing the A- and B-chains with a high yield, which has been challenging to achieve by other ion activation methods. Disulfides 27-36 insulin Bos taurus 60-67 9314099-0 1997 Fluoresceinthiocarbamyl-insulin: a potential analytical tool for the assay of disulfide bond reduction. Disulfides 78-87 insulin Bos taurus 24-31 25510931-8 2015 Insulin ions show dramatically reduced HDX levels and exchange rates, which can be attributed to decreased conformational flexibility resulting from the disulfide bonds. Disulfides 153-162 insulin Bos taurus 0-7 22125416-0 2011 Cleavage of Multiple Disulfide Bonds in Insulin via Gold Cationization and Collision-induced Dissociation. Disulfides 21-30 insulin Bos taurus 40-47 22125416-5 2011 However, the incorporation of two gold cations, regardless of oxidation state, resulted in efficient cleavage of the disulfide bonds connecting the two chains of insulin. Disulfides 117-126 insulin Bos taurus 162-169 20835491-0 2010 The application of negative ion electrospray mass spectrometry for the sequencing of underivatized disulfide-containing proteins: insulin and lysozyme. Disulfides 99-108 insulin Bos taurus 130-137 20027620-3 2010 The experimental results show that there are two anodic peaks for the oxidative damage of bovine insulin, which arise from the oxidation of the exposed disulfide bond S--S(CYS7A,CYS7B), forming sulfenic acid RSOH (1.20 V, vs. SCE), sulfinic acid RSO(2)H and sulfonic acid RSO(3)H (1.35 V, vs. SCE). Disulfides 152-161 insulin Bos taurus 97-104 20196567-6 2010 These findings were demonstrated in 14 peptides containing disulfide bonds and further by bovine insulin, which has three disulfide bonds. Disulfides 122-131 insulin Bos taurus 97-104 19383476-0 2009 Bovine insulin filaments induced by reducing disulfide bonds show a different morphology, secondary structure, and cell toxicity from intact insulin amyloid fibrils. Disulfides 45-54 insulin Bos taurus 7-14 19383476-2 2009 Insulin is a 51-residue polypeptide hormone, with its two polypeptide chains linked by one intrachain and two interchain disulfide bonds, and has long been known to self-assemble in vitro into amyloid fibrils. Disulfides 121-130 insulin Bos taurus 0-7 16774767-2 2006 Insulin is a small, predominantly alpha-helical protein consisting of 51 residues in two disulfide-linked polypeptide chains that readily assembles into amyloid fibrils under conditions of low pH and elevated temperature. Disulfides 89-98 insulin Bos taurus 0-7 15739495-2 1997 Insulin contains 51 amino acids and two intrachain disulfide bridges. Disulfides 51-60 insulin Bos taurus 0-7 15739495-4 1997 The results show that the conformation of insulin has changed before cleavage of the disulfide bonds to A and B chains. Disulfides 85-94 insulin Bos taurus 42-49 9314099-6 1997 On this basis, di-fluoresceinthiocarbamyl-insulin constitutes an analytical tool to test the capacity of biochemical preparations in the reduction of disulfide bonds. Disulfides 150-159 insulin Bos taurus 42-49 1570358-9 1992 Insulin proteolysis was inhibited by specific insulin protease inhibitors and stimulated by disulfide reducing agents. Disulfides 92-101 insulin Bos taurus 0-7 8274532-2 1993 The turbidimetric assay of thioredoxin with insulin as the disulfide substrate was optimized; by incorporation of the lag time (tau) into the calculations, linearity was maintained for a wider range of thioredoxin concentrations, and a distinction could be made between reduced and non-reduced forms. Disulfides 59-68 insulin Bos taurus 44-51 2201313-1 1990 We report the synthesis and biological evaluation of a two-chain, disulfide-linked, insulin-like compound consisting of the B-chain of bovine insulin and an A-chain corresponding to the A- and D- domains of human insulin-like growth factor-I (IGF-I) in which the A-domain amino-acid residues -Phe49-Arg50-Ser51-found in IGF-I have been replaced by -Ala-Gly-Val-, the homologous region of sheep insulin. Disulfides 66-75 insulin Bos taurus 142-149