PMID-sentid	Pub_year	Sent_text		comp_official_name	comp_offsetprotein_name	organism	prot_offset
10863933-1	2000	The 52-residue alpha/beta chimera of the epidermal growth factor-like domain in neu differentiation factor (NDFealpha/beta) has been synthesized and folded to form a three disulfide bridge (Cys182-Cys196, Cys190-Cys210, Cys212-Cys221) containing peptide.	Disulfides	172-181	neuregulin 1	Homo sapiens	80-106	
8547341-4	1996	Detailed chemical characterization of the recombinant molecule by peptide mapping in conjunction with Edman sequencing and mass spectrometry reveals that the bacterially produced recombinant neu differentiation factor preparation is properly folded and contains the correct disulfide structure.	Disulfides	274-283	neuregulin 1	Homo sapiens	191-217	
21500299-4	2011	Protein disulfide isomerase (PDI) was most effective at the reduced/oxidized glutathione ratio of 2:1 for refolding the denatured sample NRG1-beta1 with the native disulfide bonds.	Disulfides	8-17	neuregulin 1	Homo sapiens	137-141	
10670717-0	2000	Peptide map procedure using immobilized protease cartridges in tandem for disulfide linkage identification of neu differentiation factor epidermal growth factor domain.	Disulfides	74-83	neuregulin 1	Homo sapiens	110-136	
10670717-1	2000	Immobilized proteolytic enzyme cartridges were used to rapidly digest neu differentiation factor EGF domain in order to obtain improved peptide maps useful for assignment of disulfide linkages.	Disulfides	174-183	neuregulin 1	Homo sapiens	70-96	
10670717-3	2000	The entire process can be automated using a commercially available workstation; and the total time required for both proteolytic digestion and the HPLC separation can be shortened to within 1 h. Using these immobilized columns, we demonstrated that disulfide structure assignment of the EGF domains of recombinant human neu differentiation factor can be performed by isolation of individual disulfide-containing peptides followed by assignment of disulfide linkages with prompt fragmentation of peptides using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.	Disulfides	249-258	neuregulin 1	Homo sapiens	320-346