PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 11530021-4 2001 These single-tryptophan mutations induce large changes in the free energy of urea unfolding, which showed values of 1.79-7.14 kcal/mol, compared with the value for wild-type DHFR of 6.08 kcal/mol. single-tryptophan 6-23 Dihydrofolate reductase Escherichia coli 174-178