PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 29230506-4 2018 MATERIALS AND METHODS: TNFalpha-stimulated CF cells CuFi-1 and its normal counterpart NuLi-1 were pre-treated with increasing concentrations of iPA and cell viability and proliferation were assessed by MTT and BrdU assays. monooxyethylene trimethylolpropane tristearate 202-205 tumor necrosis factor Homo sapiens 23-31 33854314-7 2021 Cytotoxicity studies using MTT assay demonstrated enhanced growth inhibitory effect on lung tumor cells A549 and significant reduction of proinflammatory cytokines such as tumor necrosis factor-alpha, interleukin-6 and interleukin-10 compared to the pure drug. monooxyethylene trimethylolpropane tristearate 27-30 tumor necrosis factor Homo sapiens 172-199 33624201-4 2021 The viability and proliferation rate of SCAP treated with TNF-alpha were assessed using a colorimetric MTT assay. monooxyethylene trimethylolpropane tristearate 103-106 tumor necrosis factor Homo sapiens 58-67 30591106-3 2018 The inflammatory cell model was established by tumor necrosis factor-alpha (TNF-alpha) treating CD44+CD133+ HT29 cells and the optimal dose and reaction time of TNF-alpha were confirmed by MTT assay. monooxyethylene trimethylolpropane tristearate 189-192 tumor necrosis factor Homo sapiens 161-170 26405983-9 2015 The proliferation rate of HepG2 cells detected by MTT showed that a treatment of AdshRNA-RhoA and TNF-alpha together could strengthen the suppression ability of TNF-alpha to HepG2 cells, resulting in approximately 14.2% more than those treated with only TNF-alpha. monooxyethylene trimethylolpropane tristearate 50-53 tumor necrosis factor Homo sapiens 98-107 26804809-8 2016 Motility at scratch assay and proliferation at MTT assay of Caco2 cells displayed differential modulation by TNF-alpha and infliximab, directly or through supernatants of human intestinal fibroblasts and human peripheral blood mononuclear cells exposed to them. monooxyethylene trimethylolpropane tristearate 47-50 tumor necrosis factor Homo sapiens 109-118 26463665-7 2015 The TNF-mediated chemosensitization was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) cytotoxicity assay. monooxyethylene trimethylolpropane tristearate 116-119 tumor necrosis factor Homo sapiens 4-7 26082376-7 2015 MTT assay revealed that TNF-alpha promoted hMSC proliferation. monooxyethylene trimethylolpropane tristearate 0-3 tumor necrosis factor Homo sapiens 24-33 29085463-10 2017 The MTT cell proliferation and cytotoxicity assay revealed that TNFalpha + TGFbeta-1 treatment significantly increased cell proliferation and daunorubicin resistance, but not gemcitabine resistance. monooxyethylene trimethylolpropane tristearate 4-7 tumor necrosis factor Homo sapiens 64-72 28096970-4 2016 MATERIALS AND METHODS: The role of Akt activation in TNF-alpha cytotoxicity was investigated by MTT cell viability assay following treatment of the cells with the chemical inhibitor of Akt activation with or without TNF-alpha treatment. monooxyethylene trimethylolpropane tristearate 96-99 tumor necrosis factor Homo sapiens 53-62 26405983-9 2015 The proliferation rate of HepG2 cells detected by MTT showed that a treatment of AdshRNA-RhoA and TNF-alpha together could strengthen the suppression ability of TNF-alpha to HepG2 cells, resulting in approximately 14.2% more than those treated with only TNF-alpha. monooxyethylene trimethylolpropane tristearate 50-53 tumor necrosis factor Homo sapiens 161-170 26405983-9 2015 The proliferation rate of HepG2 cells detected by MTT showed that a treatment of AdshRNA-RhoA and TNF-alpha together could strengthen the suppression ability of TNF-alpha to HepG2 cells, resulting in approximately 14.2% more than those treated with only TNF-alpha. monooxyethylene trimethylolpropane tristearate 50-53 tumor necrosis factor Homo sapiens 161-170 21880146-6 2011 TNF-alpha induced growth inhibition/apoptosis was measured by MTT, TUNEL assay and caspase activation. monooxyethylene trimethylolpropane tristearate 62-65 tumor necrosis factor Homo sapiens 0-9 23433788-3 2013 RESULTS: Using MTT and flow cytometry, IL-13, TNF-alpha and IL-1beta pretreatment decreased Fas-induced cell death. monooxyethylene trimethylolpropane tristearate 15-18 tumor necrosis factor Homo sapiens 46-55 21520161-5 2012 The toxicity of TNF-alpha in LNCaP and PC3 cells, with or without kinase and NF-kappaB inhibitors, was assessed by changes on viability (MTT assay) and apoptosis (loss of DNA, annexin-V binding, and caspase cleavage/activation). monooxyethylene trimethylolpropane tristearate 137-140 tumor necrosis factor Homo sapiens 16-25 26044863-6 2015 The purified human TNF-alpha was tested for its biological activity and structural analysis, using MTT assay (EC(50) of 4.1 x10E-12 M in L929 cell death assay) and circular dichroism spectropolarimetry, respectively. monooxyethylene trimethylolpropane tristearate 99-102 tumor necrosis factor Homo sapiens 19-28 21621154-12 2011 MTT and flow cytometry showed that the hTNFalpha gene markedly exerted a cell killing effect. monooxyethylene trimethylolpropane tristearate 0-3 tumor necrosis factor Homo sapiens 39-48 19304513-3 2009 The effect of SA-TNF-alpha fusion protein against L929 cells was evaluated by MTT assay. monooxyethylene trimethylolpropane tristearate 78-81 tumor necrosis factor Homo sapiens 17-26 21600167-2 2011 Through TNF-alpha induced apoptosis, the cell viability was observed by MTT assay, morphological observation and assays of apoptotic proteins as indicators of apoptosis. monooxyethylene trimethylolpropane tristearate 72-75 tumor necrosis factor Homo sapiens 8-17 20627024-5 2010 The inhibitory effect of fusion protein upon cytotoxicity of TNF-alpha was detected by methyl thiazolyl tetrazolium (MTT) assay before and after the fusion protein incubated in MMP or peritoneal fluid from endometriosis patients. monooxyethylene trimethylolpropane tristearate 117-120 tumor necrosis factor Homo sapiens 61-70 18338637-3 2007 The viability of cells treated with TNFalpha was measured by MTT method. monooxyethylene trimethylolpropane tristearate 61-64 tumor necrosis factor Homo sapiens 36-44 17974273-6 2007 Furthermore, tumor necrosis factor alpha (TNFalpha)-induced cytotoxicity was evaluated by 3-(4, 5-dimethythiazolyl) -2, -diphenyl-2H-tetrazolium-bromide (MTT) assay; Matrix metalloproteinase secretion was detected by zymography; Angiogenic ability in vitro was analyzed by culturing T3A cells in three-dimensional Matrigel plug. monooxyethylene trimethylolpropane tristearate 154-157 tumor necrosis factor Homo sapiens 13-40 17764703-1 2007 When cardiomyocytes were subjected to hypoxia, tumor necrosis factor-alpha (TNF-alpha; 3-50 ng/ml) or adenosine (1-100 microM), decreased hypoxic damage as was detected by lactate dehydrogenase (LDH) release, MTT (3-[4,5-Dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) absorbance, ROS (reactive oxygen species) measurement or desmin immunostaining. monooxyethylene trimethylolpropane tristearate 209-212 tumor necrosis factor Homo sapiens 47-74 17764703-1 2007 When cardiomyocytes were subjected to hypoxia, tumor necrosis factor-alpha (TNF-alpha; 3-50 ng/ml) or adenosine (1-100 microM), decreased hypoxic damage as was detected by lactate dehydrogenase (LDH) release, MTT (3-[4,5-Dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) absorbance, ROS (reactive oxygen species) measurement or desmin immunostaining. monooxyethylene trimethylolpropane tristearate 209-212 tumor necrosis factor Homo sapiens 76-85 17974273-6 2007 Furthermore, tumor necrosis factor alpha (TNFalpha)-induced cytotoxicity was evaluated by 3-(4, 5-dimethythiazolyl) -2, -diphenyl-2H-tetrazolium-bromide (MTT) assay; Matrix metalloproteinase secretion was detected by zymography; Angiogenic ability in vitro was analyzed by culturing T3A cells in three-dimensional Matrigel plug. monooxyethylene trimethylolpropane tristearate 154-157 tumor necrosis factor Homo sapiens 42-50 15136050-6 2004 In this study, we found that aspirin inhibited TNF-alpha (10 ng/ml)-induced MCP-1 and IL-8 expression at the RNA and protein levels in human umbilical vein endothelial cells (HUVECs), monocyte adhesion and transmigration, and that its inhibitory effects were not due to decreased HUVEC viability as assessed by MTT test. monooxyethylene trimethylolpropane tristearate 311-314 tumor necrosis factor Homo sapiens 47-56 17286914-3 2007 The interaction between TBP and TRBP was determined by non-denatured PAGE and the inhibitory effect of TBP and TRBP on TNF-alpha cytotoxicity against U937 was carried out by MTT colorimetry. monooxyethylene trimethylolpropane tristearate 174-177 tumor necrosis factor Homo sapiens 119-128 16511915-5 2006 Cell proliferation of FLS induced by TNF-alpha was determined by MTT assay. monooxyethylene trimethylolpropane tristearate 65-68 tumor necrosis factor Homo sapiens 37-46 16511915-8 2006 MTT assay revealed that simvastatin could inhibit proliferation of FLS induced by TNF-alpha. monooxyethylene trimethylolpropane tristearate 0-3 tumor necrosis factor Homo sapiens 82-91 16546723-2 2006 METHODS: The PLC-gamma1 pathway was blocked by U73122 in SWO cells, and the inhibitory effect of TNF-alpha on SWO glioma cell proliferation with or without U73122 treatment was investigated by MTT assay. monooxyethylene trimethylolpropane tristearate 193-196 tumor necrosis factor Homo sapiens 97-106 17013529-4 2006 MTT cell proliferation assay and flow cytometric analysis showed a significant increase in apoptotic and necrotic cells and decrease of proliferation in all cell lines after combine therapy with hTNFalpha expression plus thymidine kinase/GCV suicide system. monooxyethylene trimethylolpropane tristearate 0-3 tumor necrosis factor Homo sapiens 195-204 17454250-4 2007 MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) cell viability decreased significantly (p < .05) from 0.00005 to 0.05 mg/ml after 24 h. The proinflammatory mediators of inflammation cytokines interleukin (IL)-6, IL-8, tumor necrosis factor (TNF)-alpha, IL-10, and IL-1beta were also assessed. monooxyethylene trimethylolpropane tristearate 0-3 tumor necrosis factor Homo sapiens 240-273 16419152-4 2005 The cytotoxicity of the combination of TRAIL, TNF alpha, Fas ligand, and E3330 increased synergistically in a dose-dependent manner compared to either E3330 alone in all HCC cell lines by MTT assay. monooxyethylene trimethylolpropane tristearate 188-191 tumor necrosis factor Homo sapiens 46-55 15163401-4 2003 The bioactivities of wild type TNF-alpha and the TNF-alpha mutein 471 were detected by MTT colorimetry. monooxyethylene trimethylolpropane tristearate 87-90 tumor necrosis factor Homo sapiens 49-58 12717360-11 2003 The percent cytotoxicity in MTT assays using TNF, melphalan, and TNF + melphalan against NIH1286 were 0%, 51-59%, and 74-81%, respectively, and against HDMVEC were 28%, 16-23%, and 6-13%, respectively. monooxyethylene trimethylolpropane tristearate 28-31 tumor necrosis factor Homo sapiens 45-48 12717360-11 2003 The percent cytotoxicity in MTT assays using TNF, melphalan, and TNF + melphalan against NIH1286 were 0%, 51-59%, and 74-81%, respectively, and against HDMVEC were 28%, 16-23%, and 6-13%, respectively. monooxyethylene trimethylolpropane tristearate 28-31 tumor necrosis factor Homo sapiens 65-68 11191284-6 2000 The combination of TNFalpha+IFNgamma induced a significant decrease in cell viability as judged by methyltetrazoleum (MTT) metabolism which decreased to median 68% of unexposed cultures (P < 0.01). monooxyethylene trimethylolpropane tristearate 118-121 tumor necrosis factor Homo sapiens 19-27 11466216-8 2001 Although TNF alpha had no effect on [(3)H]thymidine incorporation irrespective of the presence of the growth factor, MTT metabolism was higher in F5,6 granulosa cells cultured for 24 h with both TNF alpha and TGF alpha than with either cytokine alone. monooxyethylene trimethylolpropane tristearate 117-120 tumor necrosis factor Homo sapiens 195-204 12973924-2 2003 The HL-60 cell lines were stimulated in vitro with LPS intravenously for different time to establish the cellular model of inflammation and simultaneously induce in vivo inflammation animal model by LPS The cytotoxic effects of soluble TNF alpha were checked using MTT colorimetric method to determine the rate of cell proliferation. monooxyethylene trimethylolpropane tristearate 265-268 tumor necrosis factor Homo sapiens 236-245 7886023-3 1994 After 4 days of culture TNF-alpha alone induced a loss of trophoblast viability as measured by both metabolic capacity (MTT reduction) and DNA content. monooxyethylene trimethylolpropane tristearate 120-123 tumor necrosis factor Homo sapiens 24-33 10444519-11 1999 Coexposure to TNF-alpha and NTBI, but not to TBI, induced MDA accumulation and greater cytotoxicity (MTT and lactate dehydrogenase release) than TNF-alpha alone. monooxyethylene trimethylolpropane tristearate 101-104 tumor necrosis factor Homo sapiens 14-23 10374580-3 1998 Activities of tumor necrosis factor (TNF) and interleukin 6 (IL-6) in its supernatant were determined with isotope labelling method and MTT colorimetry, respectively. monooxyethylene trimethylolpropane tristearate 136-139 tumor necrosis factor Homo sapiens 14-35 9577951-5 1998 Similar findings were observed with respect to 45Ca2+ uptake and MTT metabolism in TNF-alpha-pretreated cells that were subjected to prolonged hypoxia. monooxyethylene trimethylolpropane tristearate 65-68 tumor necrosis factor Homo sapiens 83-92 8679222-5 1996 In parallel experiments, TNF alpha, rTNF alpha-p55, and htr-9 induced human TSMC proliferation as measured by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. monooxyethylene trimethylolpropane tristearate 177-180 tumor necrosis factor Homo sapiens 25-34 11044363-6 2000 Human lines UM-SCC-9, 11B and 38, previously shown to exhibit constitutive activation of NF-kappaB, were found to be highly resistant to growth inhibition by up to 10(4)U/ml of TNF-alpha in 5 day MTT assay. monooxyethylene trimethylolpropane tristearate 196-199 tumor necrosis factor Homo sapiens 177-186 10871651-4 2000 IFN-gamma and increasing doses of TNF-alpha resulted in decreasing viability of trophoblast on uncoated as well as fibronectin-coated dishes, as shown by 3-[4, 5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) assays, but for each TNF/IFN treatment condition viability on fibronectin was higher (P < 0.001). monooxyethylene trimethylolpropane tristearate 218-221 tumor necrosis factor Homo sapiens 34-43 10683966-0 1997 [Improved MTT colorimetric assay for serum TNF activity]. monooxyethylene trimethylolpropane tristearate 10-13 tumor necrosis factor Homo sapiens 43-46 10683966-4 1997 We used this improved MTT colorimetric assay for measuring TNF activity of peripheral blood serum of healthy persons. monooxyethylene trimethylolpropane tristearate 22-25 tumor necrosis factor Homo sapiens 59-62 10743050-3 1997 MTT colorimetric method was used to study the cytotoxicity of TNF-m on the three gastric cancer cell lines in vitro. monooxyethylene trimethylolpropane tristearate 0-3 tumor necrosis factor Homo sapiens 62-65 34367277-4 2021 Methods: First, we determined the dose of TNF-alpha in HepG2 cells that has no cytotoxic effect by using MTT assays and that inhibits Epo synthesis by qRT-PCR and ELISA. monooxyethylene trimethylolpropane tristearate 105-108 tumor necrosis factor Homo sapiens 42-51 1478693-4 1992 Enhancement of TNF-alpha lysis by LPS occurred in several assays for determining TNF-alpha, including MTT cleavage, crystal violet staining and lactate dehydrogenase release. monooxyethylene trimethylolpropane tristearate 102-105 tumor necrosis factor Homo sapiens 15-24 33034821-4 2021 The effect of TNF-alpha on hepatoma cells was shown using MTT and Annexin-V/PI staining with flow cytometer analyses. monooxyethylene trimethylolpropane tristearate 58-61 tumor necrosis factor Homo sapiens 14-23 8243565-5 1993 TNF-alpha-induced apoptosis (10(4) U/mL) as measured by multiparameter flow cytometry (propidium iodide [PI]) paralleled the functional decrease in cell viability (MTT assay) of 20 +/- 3% after 24 hours up to a maximum of 50 +/- 4% after 48 hours. monooxyethylene trimethylolpropane tristearate 164-167 tumor necrosis factor Homo sapiens 0-9 8360707-3 1993 Most of glioblastoma cell lines were resistant to cytotoxic activity of TNF in a MTT assay at concentrations below 16 U/ml for 72 h exposure. monooxyethylene trimethylolpropane tristearate 81-84 tumor necrosis factor Homo sapiens 72-75