PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 17553346-5 2007 The anti-TNF-alpha biological activity assay of fusion protein was performed with L929 cells and detected with MTT colorimetry. monooxyethylene trimethylolpropane tristearate 111-114 tumor necrosis factor Mus musculus 9-18 8613675-8 1996 The assay can be performed in a few hours and has the advantage over the current MTT and 51Cr-released assays that kinetic studies of TNF-alpha toxicity are possible since it permits multiple, sequenced measurements of cell viability during the incubation of the sample. monooxyethylene trimethylolpropane tristearate 81-84 tumor necrosis factor Mus musculus 134-143 17802880-4 2007 The TNF-alpha secretion inhibitory activity of the mouse peritoneal macrophages was evaluated by MTT methods. monooxyethylene trimethylolpropane tristearate 97-100 tumor necrosis factor Mus musculus 4-13 15196381-3 2004 sTNFRI in cell culture supernatant was detected by ELISA and sTNFRI blockage of TNF-alpha cytotoxicity in L929 cells evaluated by MTT assay. monooxyethylene trimethylolpropane tristearate 130-133 tumor necrosis factor Mus musculus 80-89 12561198-7 2002 The activity of inhibiting TNF-alpha was detected with L929 cell and MTT method. monooxyethylene trimethylolpropane tristearate 69-72 tumor necrosis factor Mus musculus 27-36 11135695-5 2000 One hour predisposed MTT trial in control with parental antibody HAb25 affirmed that hscFv25-TNFalpha was cytotoxic to targeted cell SMMC-7721 with the IC(50) to be 7.1 microg/ml. monooxyethylene trimethylolpropane tristearate 21-24 tumor necrosis factor Mus musculus 93-101 15088640-7 2004 Also more TNF-alpha in supernatant that was measured by MTT via L929 cell was produced by macrophages under the inducing of 17beta-estradiol. monooxyethylene trimethylolpropane tristearate 56-59 tumor necrosis factor Mus musculus 10-19 8920943-1 1996 Tumor necrosis factor alpha mediated cell death in L929 cells correlates with a late increase in reduction of the superoxide scavenger 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), suggesting an increase in MTT reduction per viable cell. monooxyethylene trimethylolpropane tristearate 197-200 tumor necrosis factor Mus musculus 0-27 8920943-1 1996 Tumor necrosis factor alpha mediated cell death in L929 cells correlates with a late increase in reduction of the superoxide scavenger 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), suggesting an increase in MTT reduction per viable cell. monooxyethylene trimethylolpropane tristearate 229-232 tumor necrosis factor Mus musculus 0-27 8920943-3 1996 Within 36 hrs TNF promoted a 7-fold increase in the reduction of MTT in TNF-sensitive cells. monooxyethylene trimethylolpropane tristearate 65-68 tumor necrosis factor Mus musculus 14-17 8920943-3 1996 Within 36 hrs TNF promoted a 7-fold increase in the reduction of MTT in TNF-sensitive cells. monooxyethylene trimethylolpropane tristearate 65-68 tumor necrosis factor Mus musculus 72-75 8920943-6 1996 The remaining TNF-resistant clone was sensitive to ceramide, displaying an increase in MTT reduction. monooxyethylene trimethylolpropane tristearate 87-90 tumor necrosis factor Mus musculus 14-17 8317331-6 1993 Furthermore, the MTT assay was more sensitive to TNF-alpha than the 3H-TdR assay. monooxyethylene trimethylolpropane tristearate 17-20 tumor necrosis factor Mus musculus 49-58 29890232-6 2018 In this animal model, it was proposed that Mtt venom induces inflammation with the release of mediators such as TNF-alpha, in response to the toxins. monooxyethylene trimethylolpropane tristearate 43-46 tumor necrosis factor Mus musculus 112-121 8019909-8 1993 We evaluate cytotoxicity of 1 U/ml TNF on L929 murine fibroblasts after 24 hours incubation with actinomycin D by the MTT and Cr51 release. monooxyethylene trimethylolpropane tristearate 118-121 tumor necrosis factor Mus musculus 35-38 33290843-6 2021 The biological activity of the soluble recombinant TNF-alpha was investigated using MTT assay. monooxyethylene trimethylolpropane tristearate 84-87 tumor necrosis factor Mus musculus 51-60 33290843-11 2021 The significant expression level of the recombinant protein in the soluble form, its high purity, and assessment of its biological activity showed that the expressed protein could be used in tests of ELISA and MTT to assess the activity of anti-TNF-alpha agents. monooxyethylene trimethylolpropane tristearate 210-213 tumor necrosis factor Mus musculus 245-254 27879679-5 2016 Further, H-TL1 attenuated the cytotoxicity of TNF-alpha in L929 cells as determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. monooxyethylene trimethylolpropane tristearate 154-157 tumor necrosis factor Mus musculus 46-55 28586021-4 2017 The biological activity of TNF-alpha in the extracellular fluid was then measured using an MTT assay. monooxyethylene trimethylolpropane tristearate 91-94 tumor necrosis factor Mus musculus 27-36 22916222-8 2012 TM2E3 and TRPM2-specific shRNA also protect the cells from tumor necrosis factor (TNF)-alpha-induced cell death in MTT assay. monooxyethylene trimethylolpropane tristearate 115-118 tumor necrosis factor Mus musculus 59-92 21951285-10 2011 MTT assay showed that TNF-alpha inhibits proliferation/survival of mesenchymal stem cells when the NF-kappaB signaling pathway is blocked. monooxyethylene trimethylolpropane tristearate 0-3 tumor necrosis factor Mus musculus 22-31