PMID-sentid	Pub_year	Sent_text		comp_official_name	comp_offsetprotein_name	organism	prot_offset
14599984-4	2003	MTT colorimetric assay was used to value the killing effect of lymphokine activated killer cells (LAK) activated by IL-2 on the transfected cell line and the original line.	monooxyethylene trimethylolpropane tristearate	0-3	interleukin 2	Homo sapiens	116-120	
1827689-4	1991	We here describe a shorter assay in which IL-2 and IL-3 secretion during MLR was assessed by adding the respective lymphokine-dependent cell lines either to the MLR supernatants or directly to the original MLR cultures, using the colorimetric (3-[4,5 Dimethylthiazol-2-yl]-2.5-diphenyltetrazolium bromide) (MTT) technique or the 3H-thymidine incorporation assay.	monooxyethylene trimethylolpropane tristearate	307-310	interleukin 2	Homo sapiens	42-46	
12567781-3	2002	Hela cells were infected with Adv-IL2 in vitro, the expressed IL-2 and its bioactivity in Hela cell were determined by enzyme-linked immunosorbent assay (ELISA) and 3-(4,5-dimethylthiazol-2-yl) 2,5-diphenyltetrazolium bromide (MTT) respectively.	monooxyethylene trimethylolpropane tristearate	227-230	interleukin 2	Homo sapiens	62-66	
21180073-2	2002	METHODS: Immunocytochemistry and flow cytometric analysis were used to investigate the expression of interleukin-2 receptors (IL-2Rs) by using of specific IL-2R polyclonal antibody; MTT method and 3H-TdR incorporation method were used to examine the changes of proliferation of MCF-7 cells.	monooxyethylene trimethylolpropane tristearate	182-185	interleukin 2	Homo sapiens	101-114	
9875675-7	1998	Cells grown in IL-2 and IL-7 showed higher proliferation rates than cells grown in IL-12 according to the MTT assay.	monooxyethylene trimethylolpropane tristearate	106-109	interleukin 2	Homo sapiens	15-19	
9408607-8	1997	However, transduced cells were significantly more sensitive than parental cells to these effectors in 24-hour MTT assays, most likely due to IL-2 production by the transduced targets.	monooxyethylene trimethylolpropane tristearate	110-113	interleukin 2	Homo sapiens	141-145	
8401403-3	1993	The basic experimental conditions for the MTT assay were determined by using CTLL cells from human T-lymphoma, dependent on IL-2, and human peripheral blood lymphocytes.	monooxyethylene trimethylolpropane tristearate	42-45	interleukin 2	Homo sapiens	124-128	
8445249-2	1993	This approach has been used to analyze dose-response curves (IL-2 concentration vs. level of proliferation) measured by MTT and [3H]TdR assays.	monooxyethylene trimethylolpropane tristearate	120-123	interleukin 2	Homo sapiens	61-65	
8445249-3	1993	The values of functional dissociation constants, equivalent to IL-2 concentrations giving 50% of the maximal response, depended on the cell concentration and increased from 4 to 60 pM for the [3H]TdR assay and from 40 to 140 pM for the MTT assay when the cell concentration was increased from 2 x 10(3) to 4 x 10(4) cells/well.	monooxyethylene trimethylolpropane tristearate	236-239	interleukin 2	Homo sapiens	63-67	
9776586-5	1998	Due to the different measurement procedures, the volumes of the IL-2 dilutions were 75 microl in assays with MTT and 150 microl in assays with 3H-TdR.	monooxyethylene trimethylolpropane tristearate	109-112	interleukin 2	Homo sapiens	64-68	
9776586-7	1998	In the most optimal setting the MTT assay could detect 0.6 pg IL-2/well, corresponding to 8 pg/ml.	monooxyethylene trimethylolpropane tristearate	32-35	interleukin 2	Homo sapiens	62-66	
9498162-6	1997	The present study was carried out to evaluate the efficacy of low-dose IL-2 in association with both MLT and 5-MTT.	monooxyethylene trimethylolpropane tristearate	111-114	interleukin 2	Homo sapiens	71-75	
8712699-1	1996	This study was performed to investigate the direct effects of recombinant human tumor necrosis factor (rH-TNF) and recombinant human interleukin-2 (rH-IL-2), either alone or in combination, on the cytotoxicity of 5-FU measured by MTT assay against human gastric adenocarcinoma cell lines (MKN-28 and MKN-45), and also to determine the optimal schedule for their combination.	monooxyethylene trimethylolpropane tristearate	230-233	interleukin 2	Homo sapiens	133-146	
8295259-0	1993	Determination of IL-2 and cytotoxicity of killer cells in MTT colorimetry.	monooxyethylene trimethylolpropane tristearate	58-61	interleukin 2	Homo sapiens	17-21	
3879297-0	1985	[The measurement of interleukin 1 (IL 1) and interleukin 2 (IL 2) employing the MTT assay].	monooxyethylene trimethylolpropane tristearate	80-83	interleukin 2	Homo sapiens	60-64	
26048624-4	2015	Proliferation rate of IL-2-induced cells was measured by MTT assay.	monooxyethylene trimethylolpropane tristearate	57-60	interleukin 2	Homo sapiens	22-26	
14758053-5	2004	The effect of alpha-methyl-P-tyrosine (alpha-MT), an inhibitor of TH activity, on concanavalin A (Con A)-induced interleukin-2 (IL-2) production was determined by MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide] assay.	monooxyethylene trimethylolpropane tristearate	163-166	interleukin 2	Homo sapiens	128-132	
2969933-3	1988	IL-2 in microculture supernatants was detected in a bioassay combined with rapid colorimetric visualization (cleavage of MTT).	monooxyethylene trimethylolpropane tristearate	121-124	interleukin 2	Homo sapiens	0-4	
26668654-4	2015	The present study demonstrated that IL-2 expression levels were upregulated in the NP tissues of patients with a prolapsed lumbar intervertebral disc; and a subsequent MTT assay demonstrated that IL-2 inhibits the proliferation of HNPCs in a dose-dependent manner.	monooxyethylene trimethylolpropane tristearate	168-171	interleukin 2	Homo sapiens	196-200	
26645326-8	2015	We observed that the CD25 expression exclusively on the CD3(-)CD56(+)CD25(+) NK cells was positively correlated with their cytotoxic function evaluated by the MTT test (r = 0.68), the upregulation of granzyme B (r = 0.89), IL-2 (r = 0.78) and IFN-gamma (r = 0.57), however, it was not positively correlated with FasL and caspase-8.	monooxyethylene trimethylolpropane tristearate	159-162	interleukin 2	Homo sapiens	223-227