PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 22430528-5 2012 Pretreatment with XG significantly attenuated the cell viability reduction induced by Abeta exposure in a dose dependent manner which was testified by 3-[4,5-Dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) and lactate dehydrogenase release assay. monooxyethylene trimethylolpropane tristearate 213-216 amyloid beta precursor protein Homo sapiens 86-91 27403417-4 2016 First, the experimental results show that metformin salvaged the neurons exposed to Abeta in a concentration-dependent manner with MTT and LDH assay. monooxyethylene trimethylolpropane tristearate 131-134 amyloid beta precursor protein Homo sapiens 84-89 23369217-4 2013 Decreased cell viability after amyloid beta exposure was demonstrated using MTT and live/dead assays. monooxyethylene trimethylolpropane tristearate 76-79 amyloid beta precursor protein Homo sapiens 31-43 23864927-4 2013 Cells treated with Abeta(25-35) exhibited decreased cell viability and underwent apoptosis as determined by MTT assay and TUNEL, respectively. monooxyethylene trimethylolpropane tristearate 108-111 amyloid beta precursor protein Homo sapiens 19-24 20455019-4 2010 Incubation with 10 muM Abeta for 24 h significantly inhibits cellular MTT-reduction without inducing morphological signs of enhanced cell death or increase in release of lactate dehydrogenase. monooxyethylene trimethylolpropane tristearate 70-73 amyloid beta precursor protein Homo sapiens 23-28 21504780-2 2011 We compared the activity of Abeta oligomers (synthetic and cell culture media derived) on the human SH-SY5Y neuroblastoma and C2C12 mouse myoblast cell lines in a novel, modified MTT assay. monooxyethylene trimethylolpropane tristearate 179-182 amyloid beta precursor protein Homo sapiens 28-33 20455019-6 2010 The inhibition of MTT-reduction by Abeta was due to an acceleration of MTT-formazan exocytosis. monooxyethylene trimethylolpropane tristearate 18-21 amyloid beta precursor protein Homo sapiens 35-40 14698355-7 2003 The results of the MTT assay of Abeta peptides on differentiated SH-SY5Y cells displayed a good correlation also with the in vivo results. monooxyethylene trimethylolpropane tristearate 19-22 amyloid beta precursor protein Homo sapiens 32-37 16981713-6 2006 Two scFvs with differing affinities for Abeta were studied, and both inhibited aggregation of Abeta42 as determined by thioflavin T binding assay and atomic force microscopy analysis and blocked Abeta-induced toxicity toward human neuroblastoma SH-SY5Y cells as determined by MTT and LDH release assays. monooxyethylene trimethylolpropane tristearate 276-279 amyloid beta precursor protein Homo sapiens 40-45 16981713-6 2006 Two scFvs with differing affinities for Abeta were studied, and both inhibited aggregation of Abeta42 as determined by thioflavin T binding assay and atomic force microscopy analysis and blocked Abeta-induced toxicity toward human neuroblastoma SH-SY5Y cells as determined by MTT and LDH release assays. monooxyethylene trimethylolpropane tristearate 276-279 amyloid beta precursor protein Homo sapiens 94-99 17157826-3 2007 The first method takes advantage of the unique ability of extracellularly applied Abeta oligomers to rapidly induce the exocytosis of formazan formed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT). monooxyethylene trimethylolpropane tristearate 215-218 amyloid beta precursor protein Homo sapiens 82-87 15094512-5 2004 Using the MTT assay, NT2 showed greatest susceptibility to soluble oligomeric Abeta. monooxyethylene trimethylolpropane tristearate 10-13 amyloid beta precursor protein Homo sapiens 78-83 12393269-8 2002 Thus, Abeta seems to modulate early endosomal trafficking via inhibition of the PI-3K pathway in the presence of MTT. monooxyethylene trimethylolpropane tristearate 113-116 amyloid beta precursor protein Homo sapiens 6-11 12393269-1 2002 Amyloid beta-peptide (Abeta) effectively inhibits the cellular reduction activity of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) in a variety of cultured cells. monooxyethylene trimethylolpropane tristearate 147-150 amyloid beta precursor protein Homo sapiens 22-27 12393269-3 2002 In the present study, we examined the effect of Abeta on the morphology of early endosomes, in which MTT is accumulated as MTT formazan after cellular reduction. monooxyethylene trimethylolpropane tristearate 101-104 amyloid beta precursor protein Homo sapiens 48-53 14573766-2 2003 to simultaneously enhance amyloid beta-protein (Abeta) fibrillogenesis and decrease cellular toxicity, as measured in a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction assay. monooxyethylene trimethylolpropane tristearate 182-185 amyloid beta precursor protein Homo sapiens 48-53 12393269-9 2002 Modulation of early endosomal trafficking appears to affect the cellular metabolism of MTT, causing suppression of cellular MTT reduction by Abeta. monooxyethylene trimethylolpropane tristearate 87-90 amyloid beta precursor protein Homo sapiens 141-146 12393269-9 2002 Modulation of early endosomal trafficking appears to affect the cellular metabolism of MTT, causing suppression of cellular MTT reduction by Abeta. monooxyethylene trimethylolpropane tristearate 124-127 amyloid beta precursor protein Homo sapiens 141-146 11223020-5 2001 Furthermore, only the pH 5.8 Abeta aggregates induced significant apoptotic death of PC12 cells, as indicated by a decrease in 3-(4,5-dimethylthiazol-2-yl)2,5-diphenyltetrazolium bromide (MTT) reduction and an increase in phosphatidylserine externalization. monooxyethylene trimethylolpropane tristearate 188-191 amyloid beta precursor protein Homo sapiens 29-34 11137881-4 2001 Abeta(1-42) decreased MTT reduction potently in the absence of cell death, but did not affect cellular ATP levels or lactate release. monooxyethylene trimethylolpropane tristearate 22-25 amyloid beta precursor protein Homo sapiens 0-5 11137881-6 2001 Abeta(1-42) enhanced transfer of MTT dye to the cell surface leading to cessation of MTT reduction and cell death. monooxyethylene trimethylolpropane tristearate 33-36 amyloid beta precursor protein Homo sapiens 0-5 11137881-6 2001 Abeta(1-42) enhanced transfer of MTT dye to the cell surface leading to cessation of MTT reduction and cell death. monooxyethylene trimethylolpropane tristearate 85-88 amyloid beta precursor protein Homo sapiens 0-5 9216088-3 1997 The time-dependent self-assembly of monomeric A beta into fibers was simultaneously monitored by electron microscopy, circular dichroism spectroscopy, analytical ultracentrifugation, and A beta-mediated cellular toxicity using the reduction of 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) to measure cell viability. monooxyethylene trimethylolpropane tristearate 306-309 amyloid beta precursor protein Homo sapiens 46-52 10072300-3 1999 Treatment of LAN5 human neuroblastoma cells with 10 microM beta-amyloid peptide fragment 25-35 (Abeta 25-35) for 72 h resulted in a 50% decrease in cell viability as determined by MTT incorporation and cell counts. monooxyethylene trimethylolpropane tristearate 180-183 amyloid beta precursor protein Homo sapiens 59-79 9332729-2 1997 Treatment of both hNT and NT2/D1 cells with 10(-5) M beta-amyloid peptide fragment 25-35 (A beta P) for 24 h resulted in a decrease in cell viability as determined by MTT incorporation and Trypan blue exclusion, and also induced an apoptotic morphology in hNT cells. monooxyethylene trimethylolpropane tristearate 167-170 amyloid beta precursor protein Homo sapiens 53-73 9832130-1 1998 Perhaps the most reproducible early event induced by the interaction of amyloid beta peptide (A beta) with the cell is the inhibition of cellular 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction. monooxyethylene trimethylolpropane tristearate 208-211 amyloid beta precursor protein Homo sapiens 94-100 9832130-2 1998 We recently demonstrated that cytotoxic amyloid peptides such as A beta and human amylin inhibit cellular MTT reduction by dramatically enhancing MTT formazan exocytosis. monooxyethylene trimethylolpropane tristearate 106-109 amyloid beta precursor protein Homo sapiens 65-71 9375659-2 1997 An early indicator of A beta toxicity is the inhibition of cellular 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction to MTT formazan, a widely used assay for measuring cell viability. monooxyethylene trimethylolpropane tristearate 130-133 amyloid beta precursor protein Homo sapiens 22-28 9375659-3 1997 In this report we show that A beta and other cytotoxic amyloid peptides such as human amylin dramatically enhance MTT formazan exocytosis, resulting in the inhibition of cellular MTT reduction. monooxyethylene trimethylolpropane tristearate 114-117 amyloid beta precursor protein Homo sapiens 28-34 9216088-10 1997 Wild-type A beta 1-42 and its oxidized derivative carrying a methionine sulfoxide residue at position 35 showed the highest rate of fiber formation and exerted toxic activity in the MTT assay at very low nanomolar concentrations. monooxyethylene trimethylolpropane tristearate 182-185 amyloid beta precursor protein Homo sapiens 10-16 8905718-6 1996 These results suggest that even though inhibition of MTT reduction represents an early indicator of the A beta 1-4C mediated cell injury, without other corroborating evidence, it should not be used as a measure of cell death. monooxyethylene trimethylolpropane tristearate 53-56 amyloid beta precursor protein Homo sapiens 104-110 8905718-1 1996 The reduction in 3-[4,5-dimethylthiazol]-2,5-diphenyltetrazolium bromide (MTT) to a coloured formazan compound by cultured cells has been extensively used as an in vitro model for understanding neurobiological mechanisms involved in amyloid beta-protein (A beta-mediated cell death. monooxyethylene trimethylolpropane tristearate 74-77 amyloid beta precursor protein Homo sapiens 255-261 8905718-2 1996 In primary cultures of astrocytes, very low concentrations of aggregated A beta 1-4C, but not A beta 4C-1, produced a significant inhibition in the reduction of the dye MTT. monooxyethylene trimethylolpropane tristearate 169-172 amyloid beta precursor protein Homo sapiens 73-79 8863516-2 1996 A modified 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, in which a yellow redox dye, MTT, is reduced to purple formazan, is very sensitive to the effect of A beta. monooxyethylene trimethylolpropane tristearate 73-76 amyloid beta precursor protein Homo sapiens 185-191 8863516-2 1996 A modified 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, in which a yellow redox dye, MTT, is reduced to purple formazan, is very sensitive to the effect of A beta. monooxyethylene trimethylolpropane tristearate 114-117 amyloid beta precursor protein Homo sapiens 185-191 8863516-3 1996 In primary hippocampal cultures, inhibition of MTT reduction starts within 2 h after the addition of low concentrations of A beta and reaches a plateau in 12 h. This effect of A beta is not blocked by Ca2+ channel blockers or in Ca(2+)-free medium. monooxyethylene trimethylolpropane tristearate 47-50 amyloid beta precursor protein Homo sapiens 123-129 8863516-3 1996 In primary hippocampal cultures, inhibition of MTT reduction starts within 2 h after the addition of low concentrations of A beta and reaches a plateau in 12 h. This effect of A beta is not blocked by Ca2+ channel blockers or in Ca(2+)-free medium. monooxyethylene trimethylolpropane tristearate 47-50 amyloid beta precursor protein Homo sapiens 176-182 8863516-5 1996 When A beta was washed out from the medium after 12 h, MTT reduction recovers and LDH release does not occur, suggesting that a long-lasting inhibition of the cellular redox system may be required to induce cell death. monooxyethylene trimethylolpropane tristearate 55-58 amyloid beta precursor protein Homo sapiens 5-11 32681443-6 2020 The proliferation and apoptosis of Abeta-treated SK-N-SH and SK-N-AS cells were determined via 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) or flow cytometry assays, respectively. monooxyethylene trimethylolpropane tristearate 162-165 amyloid beta precursor protein Homo sapiens 35-40 7790863-2 1995 Using rat pheochromocytoma PC12 or human epitheloid HeLa cell lines, submicromolar concentrations of the beta-AP fragments beta 1-40, beta 1-39, and beta 25-35, but not beta 1-28, were found to inhibit the reduction of the redox dye 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT). monooxyethylene trimethylolpropane tristearate 295-298 amyloid beta precursor protein Homo sapiens 105-112 7790863-3 1995 In both cell lines, the beta-AP-sensitive component represented approximately 70% of total cellular MTT reduction. monooxyethylene trimethylolpropane tristearate 100-103 amyloid beta precursor protein Homo sapiens 24-31 7790863-7 1995 These results support the hypothesis that the cellular reduction of MTT represents a specific indicator of the initial events underlying the mechanism of beta-AP toxicity. monooxyethylene trimethylolpropane tristearate 68-71 amyloid beta precursor protein Homo sapiens 154-161 33778168-5 2021 In addition, BP treatment was found to have a cytoprotective activity against Abeta-induced cell cytotoxicity as shown by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) cell toxicity assay. monooxyethylene trimethylolpropane tristearate 188-191 amyloid beta precursor protein Homo sapiens 78-83 34944492-8 2021 We used electron microscopy, circular dichroism, X-ray diffraction, solid-state NMR spectroscopy, ThT fluorescence and MTT cell toxicity assays to comprehensively investigate the physicochemical properties of the Abeta fibrils formed by the modified peptides as well as toxicity to a neuronal cell line. monooxyethylene trimethylolpropane tristearate 119-122 amyloid beta precursor protein Homo sapiens 213-218 34612337-7 2021 Moreover, the influence of SWCNT-COOH on cytotoxicity induced by Abeta was investigated by the MTT method. monooxyethylene trimethylolpropane tristearate 95-98 amyloid beta precursor protein Homo sapiens 65-70 35281253-7 2022 Furthermore, an MTT assay was applied to evaluate the anti-Abeta fibril formation function of heparin tetrasaccharide, and indicated that the heparin tetrasaccharide with the defined sequence represents a promising inhibitor of Abeta aggregation. monooxyethylene trimethylolpropane tristearate 16-19 amyloid beta precursor protein Homo sapiens 59-64 32871216-6 2020 MTT assay was used to examine the viability of Abeta-treated SH-SY5Y and SK-N-SH cells. monooxyethylene trimethylolpropane tristearate 0-3 amyloid beta precursor protein Homo sapiens 47-52 30279351-8 2018 Isothermal titration calorimetry (ITC) was used to measure binding affinity to Abeta, thereafter 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) based tissue viability assay and impedance-based viability assay on SH-SY5Y cells were applied to monitor Abeta toxicity and protective effects of the compounds. monooxyethylene trimethylolpropane tristearate 159-162 amyloid beta precursor protein Homo sapiens 79-84 28660722-5 2017 They productively reversed Abeta-induced mitochondrial dysfunctions such as mitochondrial membrane potential loss (JC-1 assay), toxicity (MTT assay), and ATP reduction (ATP assay) in addition to increased cell viabilities. monooxyethylene trimethylolpropane tristearate 138-141 amyloid beta precursor protein Homo sapiens 27-32