PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 18634106-7 1997 This was due to the fact that leucine was released into the broth from plasmid containing cells, which enabled some cells to grow without the plasmid containing the LEU2 auxotrophic complementation selection marker. Leucine 30-37 3-isopropylmalate dehydrogenase Saccharomyces cerevisiae S288C 165-169 2696664-5 1989 It is established that the frequency of spontaneous reversions of the leu2 gene in Saccharomyces cerevisiae strain NA3-24 increases when the cells are cultivated on the culture medium with different concentrations of leucine. Leucine 217-224 3-isopropylmalate dehydrogenase Saccharomyces cerevisiae S288C 70-74 7483835-3 1995 One-step gene disruption of ORF5, via in vivo homologous recombination between native plasmid k2 and a transfer vector employing the Saccharomyces cerevisiae LEU2 gene fused to the k2 UCS5 element, yielded Leu+ transformants at high frequencies. Leucine 206-209 3-isopropylmalate dehydrogenase Saccharomyces cerevisiae S288C 158-162 8162183-1 1994 Phenotypically and genotypically (leu2-3, 112) Leu- cells of Saccharomyces cerevisiae gave rise to small colonies on medium devoid of leucine. Leucine 134-141 3-isopropylmalate dehydrogenase Saccharomyces cerevisiae S288C 34-38 2063623-2 1991 It was shown that a decrease in the amount of adenine (from 500 to 0 mg l-1) or leucine (from 300 to 0.3 mg l-1) in the medium, simultaneously with the transition from repression to derepression of the biosynthesis of these metabolites, resulted in a 15- to 150-fold increase in the reversion rate of genes ade 2 and leu2, respectively, for different strains. Leucine 80-87 3-isopropylmalate dehydrogenase Saccharomyces cerevisiae S288C 317-321 24173418-0 1983 Leucine biosynthesis in yeast : Identification of two genes (LEU4, LEU5) that affect alpha-Isopropylmalate synthase activity and evidence that LEU1 and LEU2 gene expression is controlled by alpha-Isopropylmalate and the product of a regulatory gene. Leucine 0-7 3-isopropylmalate dehydrogenase Saccharomyces cerevisiae S288C 152-156 2976118-6 1988 SGY-484 was transformed to Leu+ with either the C. albicans or Saccharomyces cerevisiae LEU2 gene. Leucine 27-30 3-isopropylmalate dehydrogenase Saccharomyces cerevisiae S288C 88-92 2823102-1 1987 Although the majority of genes for amino acid biosynthesis which have been examined are under general amino acid control, LEU1 and LEU2 of Saccharomyces cerevisiae respond specifically to leucine. Leucine 188-195 3-isopropylmalate dehydrogenase Saccharomyces cerevisiae S288C 131-135 2897248-2 1987 1983) was shown to be regulated, when transferred into Saccharomyces cerevisiae, by leucine and threonine in the medium, as in the case of LEU2 gene of S. cerevisiae. Leucine 84-91 3-isopropylmalate dehydrogenase Saccharomyces cerevisiae S288C 139-143 2897248-5 1987 Comparison of the regulatory region of C-LEU2 with those of LEU1 and LEU2 suggested a few short consensus sequences which are involved in regulation of gene expression by leucine and threonine in the medium. Leucine 171-178 3-isopropylmalate dehydrogenase Saccharomyces cerevisiae S288C 41-45 2897248-5 1987 Comparison of the regulatory region of C-LEU2 with those of LEU1 and LEU2 suggested a few short consensus sequences which are involved in regulation of gene expression by leucine and threonine in the medium. Leucine 171-178 3-isopropylmalate dehydrogenase Saccharomyces cerevisiae S288C 69-73 6391880-5 1984 The resulting plasmid was able to complement a yeast GAL1-mutant and galactokinase synthesis in yeast was controlled, via the LEU2 regulatory system, by the levels of leucine and threonine in the growth medium. Leucine 167-174 3-isopropylmalate dehydrogenase Saccharomyces cerevisiae S288C 126-130 3549446-1 1987 The LEU3 gene of the yeast Saccharomyces cerevisiae, which is involved in the regulation of at least two LEU structural genes (LEU1 and LEU2), has been cloned by complementation of leu3 mutations and shown to reside within a 5.6-kb fragment. Leucine 4-7 3-isopropylmalate dehydrogenase Saccharomyces cerevisiae S288C 136-140 3549446-3 1987 It also restored approximately wild-type levels of isopropylmalate isomerase (LEU1) and beta-isopropylmalate dehydrogenase (LEU2), which were strongly reduced when exogenous leucine was supplied. Leucine 174-181 3-isopropylmalate dehydrogenase Saccharomyces cerevisiae S288C 124-128 24173418-4 1983 The properties of mutants affecting the biosynthesis of leucine and its regulation suggest that the expression of LEU1 and LEU2 (structural genes encoding isopropylmalate isomerase and beta-isopropylmalate dehydrogenase, respectively) is controlled by a complex of a-isopropylmalate and a regulatory element (the LEU3 gene product). Leucine 56-63 3-isopropylmalate dehydrogenase Saccharomyces cerevisiae S288C 123-127 6796588-5 1982 Despite the different genetic arrangements, regulation of LEU2 gene expression by leucine and leucine plus threonine was normal. Leucine 82-89 3-isopropylmalate dehydrogenase Saccharomyces cerevisiae S288C 58-62 6406836-3 1983 When the fusion was placed into yeast cells, beta-galactosidase was expressed under the same regulatory pattern as the original leu2 gene product: its synthesis was repressed in the presence of leucine and threonine. Leucine 194-201 3-isopropylmalate dehydrogenase Saccharomyces cerevisiae S288C 128-132 7035828-2 1981 Five of forty hybrid plasmids containint Physarum sequences transform leu2- yeast to Leu+ at high frequency. Leucine 85-88 3-isopropylmalate dehydrogenase Saccharomyces cerevisiae S288C 70-74 21919885-3 2012 One corresponded to LEU2 and functions by removing the dependency of the leu2 mutant host strain on uptake of extracellular leucine. Leucine 124-131 3-isopropylmalate dehydrogenase Saccharomyces cerevisiae S288C 20-24 7000755-1 1980 The repression of beta-isopropylmalate dehydrogenase, the LEU2 gene product, by leucine and leucine plus threonine was unaffected by the transposition of LEU2 from its original locus on chromosome III to a new locus within the ribosomal deoxyribonucleic acid gene cluster on chromosome XII. Leucine 80-87 3-isopropylmalate dehydrogenase Saccharomyces cerevisiae S288C 58-62 7000755-1 1980 The repression of beta-isopropylmalate dehydrogenase, the LEU2 gene product, by leucine and leucine plus threonine was unaffected by the transposition of LEU2 from its original locus on chromosome III to a new locus within the ribosomal deoxyribonucleic acid gene cluster on chromosome XII. Leucine 92-99 3-isopropylmalate dehydrogenase Saccharomyces cerevisiae S288C 58-62 28595601-5 2017 Among the defective markers commonly used in Saccharomyces cerevisiae is leu2-d, an allele of LEU2 which is involved in leucine metabolism. Leucine 120-127 3-isopropylmalate dehydrogenase Saccharomyces cerevisiae S288C 73-77 28595601-5 2017 Among the defective markers commonly used in Saccharomyces cerevisiae is leu2-d, an allele of LEU2 which is involved in leucine metabolism. Leucine 120-127 3-isopropylmalate dehydrogenase Saccharomyces cerevisiae S288C 94-98 403073-3 1977 A double mutant (prc 1- leu2-) lacking carboxypeptidase Y and auxotrophic for leucine is able to grow on the peptide benzyloxycarbonylglycylleucine (Cbz-Gly-Leu) as sole nitrogen source, indicating the existence of a second carboxypeptidase. Leucine 78-85 3-isopropylmalate dehydrogenase Saccharomyces cerevisiae S288C 24-28 18071269-3 2007 The ero1-1 leu2 strain does not grow in standard synthetic complete medium at 30 degrees C, a defect that can be remedied by increasing the L-leucine concentration in the medium or by transforming the ero1-1 leu2 strain with the LEU2 wild-type allele. Leucine 140-149 3-isopropylmalate dehydrogenase Saccharomyces cerevisiae S288C 11-15 18071269-5 2007 The leucine transporter Bap2p exhibits a dramatic decrease in stability in an ero1-1 strain, which may account for the pronounced leucine demand observed in the ero1-1 leu2 mutant. Leucine 4-11 3-isopropylmalate dehydrogenase Saccharomyces cerevisiae S288C 168-172 17688423-4 2007 The effect of LEU2 expression on growth and lipid accumulation could be reproduced by adding large amounts of leucine to the culture medium, indicating that the effect was not due to Leu2p (beta-isopropylmalate dehydrogenase) itself, but rather to leucine biosynthesis. Leucine 110-117 3-isopropylmalate dehydrogenase Saccharomyces cerevisiae S288C 14-18 17573937-3 2007 Two of the "rescuing" genes, BAP2 and TAT1, are related to transport of leucine, and one, LEU2, to synthesis of leucine, showing that sensitivity to SC medium is associated with the leu2 mutation. Leucine 72-79 3-isopropylmalate dehydrogenase Saccharomyces cerevisiae S288C 182-186 17573937-3 2007 Two of the "rescuing" genes, BAP2 and TAT1, are related to transport of leucine, and one, LEU2, to synthesis of leucine, showing that sensitivity to SC medium is associated with the leu2 mutation. Leucine 112-119 3-isopropylmalate dehydrogenase Saccharomyces cerevisiae S288C 90-94 17573937-3 2007 Two of the "rescuing" genes, BAP2 and TAT1, are related to transport of leucine, and one, LEU2, to synthesis of leucine, showing that sensitivity to SC medium is associated with the leu2 mutation. Leucine 112-119 3-isopropylmalate dehydrogenase Saccharomyces cerevisiae S288C 182-186 15548001-3 2004 Using the yeast three-hybrid assay, the glycosynthase activity of the E197A mutant of the Cel7B from Humicola insolens was linked to transcription of a LEU2 reporter gene, making cell growth dependent on glycosynthase activity in the absence of leucine. Leucine 245-252 3-isopropylmalate dehydrogenase Saccharomyces cerevisiae S288C 152-156