PMID-sentid	Pub_year	Sent_text		comp_official_name	comp_offsetprotein_name	organism	prot_offset
22902860-0	2012	Degradation of the starch components amylopectin and amylose by barley alpha-amylase 1: role of surface binding site 2.	Starch	19-25	LOC548210	Hordeum vulgare	71-86	
7901200-0	1993	Site-directed mutagenesis of histidine 93, aspartic acid 180, glutamic acid 205, histidine 290, and aspartic acid 291 at the active site and tryptophan 279 at the raw starch binding site in barley alpha-amylase 1.	Starch	167-173	LOC548210	Hordeum vulgare	197-212	
25661878-8	2015	Taken together, the findings indicate that the two SBSs act in concert to localize AMY1 to the starch granule surface and that SBS2 works synergistically with the active site in the degradation of amylopectin.	Starch	95-101	LOC548210	Hordeum vulgare	83-87	
19606835-0	2009	Two secondary carbohydrate binding sites on the surface of barley alpha-amylase 1 have distinct functions and display synergy in hydrolysis of starch granules.	Starch	143-149	LOC548210	Hordeum vulgare	66-81	
19606835-5	2009	Compared to that of wild-type AMY1, the K(d) of starch granule binding by the SBS1 W278A, W279A, and W278A/W279A mutants thus increased 15-35 times; furthermore, the k(cat)/K(m) of W278A/W279A was 2%, whereas both affinity and activity for Y380A at SBS2 were 10% of the wild-type values.	Starch	48-54	LOC548210	Hordeum vulgare	30-34	
14660599-5	2004	Y105A dominates in dual subsite -6/+4 [Y105A/T212(Y/W)]AMY1 mutants having almost retained and low activity on starch and oligosaccharides, respectively.	Starch	111-117	LOC548210	Hordeum vulgare	55-59	
16403494-9	2006	Remarkably, at low concentration (2 nM), AMY1-SBD hydrolysed barley starch granules 15-fold faster than rAMY1, while higher amounts of AMY-SBD caused molecular overcrowding of the starch granule surface.	Starch	68-74	LOC548210	Hordeum vulgare	41-45	
16403494-9	2006	Remarkably, at low concentration (2 nM), AMY1-SBD hydrolysed barley starch granules 15-fold faster than rAMY1, while higher amounts of AMY-SBD caused molecular overcrowding of the starch granule surface.	Starch	180-186	LOC548210	Hordeum vulgare	41-45	
16403494-7	2006	AMY1-SBD showed a 2-fold increased activity for soluble starch at low (0.5%) but not at high (1%) concentration.	Starch	56-62	LOC548210	Hordeum vulgare	0-4	
12423336-4	2002	Compared to wild-type, Met53Glu/Asp AMY1 displayed 117/90% activity towards insoluble Blue Starch, and Met53Ala/Ser/Gly 76/58/38%, but Met53Tyr/Trp only 0.9/0.1%, even though both Asp and Trp occur frequently at this position in family 13.	Starch	91-97	LOC548210	Hordeum vulgare	36-40	
12906821-0	2003	Sugar tongs get a grip on the starch granule in barley alpha-amylase 1.	Starch	30-36	LOC548210	Hordeum vulgare	55-70