PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 20363286-5 2010 The phosphorylation state of specific serine, threonine and tyrosine residues of the enzyme plays a pivotal role in regulation of eNOS activity. Threonine 46-55 nitric oxide synthase 3 Homo sapiens 130-134 20211863-8 2010 Ectopic expression of PMCA in endothelial cells resulted in an increase in phosphorylation of the residue Thr-495 of endogenous eNOS. Threonine 106-109 nitric oxide synthase 3 Homo sapiens 128-132 19036824-0 2009 Norfuraneol dephosphorylates eNOS at threonine 495 and enhances eNOS activity in human endothelial cells. Threonine 37-46 nitric oxide synthase 3 Homo sapiens 29-33 16270749-1 2005 Activity of endothelial nitric oxide synthase (eNOS) is modulated by protein-protein interaction and phosphorylation at specific serine or threonine residues. Threonine 139-148 nitric oxide synthase 3 Homo sapiens 12-45 16898954-1 2006 BACKGROUND: Endothelial nitric oxide synthase (eNOS) activity in endothelial cells is regulated by post-translational phosphorylation of critical serine, threonine and tyrosine residues in response to a variety of stimuli. Threonine 154-163 nitric oxide synthase 3 Homo sapiens 12-45 16898954-1 2006 BACKGROUND: Endothelial nitric oxide synthase (eNOS) activity in endothelial cells is regulated by post-translational phosphorylation of critical serine, threonine and tyrosine residues in response to a variety of stimuli. Threonine 154-163 nitric oxide synthase 3 Homo sapiens 47-51 16126727-8 2005 The mutant thrombin evoked a Ca2+ spark and progressive phosphorylation of Src family kinases at tyrosine 416 and NOS3 only at threonine 495. Threonine 127-136 nitric oxide synthase 3 Homo sapiens 114-118 17922342-0 2007 Cigarette smoke-induced alterations in endothelial nitric oxide synthase phosphorylation: role of protein kinase C. Endothelial nitric oxide synthase (eNOS) is regulated by phosphorylation of Ser(1177) and Thr(495), which affects NO bioavailability. Threonine 206-209 nitric oxide synthase 3 Homo sapiens 116-149 16966328-3 2006 In the present study, the homologous serines of both nNOS (Ser-1176) and endothelial nitric-oxide synthase (eNOS) (Ser-942) were mutated to threonine and alanine. Threonine 140-149 nitric oxide synthase 3 Homo sapiens 73-106 16497987-4 2006 Ox-PAPC treatment of EC induced a dose- and time-dependent activation of eNOS, as measured by phosphorylation of serine 1177, dephosphorylation of threonine 495, and the conversion of L-arginine to L-citrulline. Threonine 147-156 nitric oxide synthase 3 Homo sapiens 73-77 16270749-1 2005 Activity of endothelial nitric oxide synthase (eNOS) is modulated by protein-protein interaction and phosphorylation at specific serine or threonine residues. Threonine 139-148 nitric oxide synthase 3 Homo sapiens 47-51 15319431-7 2004 In particular, AbetaP enhances eNOS phosphorylation on threonine 495 and serine 116 and reduces acetylcholine-induced phosphorylation on serine 1177. Threonine 55-64 nitric oxide synthase 3 Homo sapiens 31-35 15721870-3 2005 METHODS AND RESULTS: In unstimulated human endothelial cells, eNOS Thr(495) was constitutively phosphorylated. Threonine 67-70 nitric oxide synthase 3 Homo sapiens 62-66 15721870-5 2005 ox-LDL, but not native LDL, induced a time- and concentration-dependent decrease in the phosphorylation of eNOS on Thr(495). Threonine 115-118 nitric oxide synthase 3 Homo sapiens 107-111 15721870-9 2005 In COS-7 cells, a T495A eNOS mutant generated significantly more O(2)(-) than a T495D mutant did, indicating that the dephosphorylation of Thr(495) alone can increase O(2)(-) production by eNOS. Threonine 139-142 nitric oxide synthase 3 Homo sapiens 24-28 15721870-9 2005 In COS-7 cells, a T495A eNOS mutant generated significantly more O(2)(-) than a T495D mutant did, indicating that the dephosphorylation of Thr(495) alone can increase O(2)(-) production by eNOS. Threonine 139-142 nitric oxide synthase 3 Homo sapiens 189-193 15721870-10 2005 However, although the dephosphorylation of Thr(495) in histamine-stimulated endothelial cells enhanced the binding of calmodulin to eNOS, calmodulin no longer bound to eNOS from ox-LDL-treated endothelial cells. Threonine 43-46 nitric oxide synthase 3 Homo sapiens 132-136 12482742-4 2003 Two amino acids seem to be particularly important in regulating eNOS activity and these are a serine residue in the reductase domain (Ser(1177)) and a threonine residue (Thr(495)) located within the CaM-binding domain. Threonine 151-160 nitric oxide synthase 3 Homo sapiens 64-68 12952971-0 2003 Phosphorylation of threonine 497 in endothelial nitric-oxide synthase coordinates the coupling of L-arginine metabolism to efficient nitric oxide production. Threonine 19-28 nitric oxide synthase 3 Homo sapiens 36-69 12482742-4 2003 Two amino acids seem to be particularly important in regulating eNOS activity and these are a serine residue in the reductase domain (Ser(1177)) and a threonine residue (Thr(495)) located within the CaM-binding domain. Threonine 170-173 nitric oxide synthase 3 Homo sapiens 64-68 12482742-5 2003 Simultaneous alterations in the phosphorylation of Ser(1177) and Thr(495) in response to a variety of stimuli are regulated by a number of kinases and phosphatases that continuously associate with and dissociate from the eNOS signaling complex. Threonine 65-68 nitric oxide synthase 3 Homo sapiens 221-225 11397791-0 2001 Phosphorylation of Thr(495) regulates Ca(2+)/calmodulin-dependent endothelial nitric oxide synthase activity. Threonine 19-22 nitric oxide synthase 3 Homo sapiens 66-99 11397791-2 2001 In the present study, we assessed whether the agonist-induced (Ca(2+)-dependent, high-output) activation of eNOS is associated with changes in the phosphorylation of Thr(495) in the calmodulin (CaM)-binding domain. Threonine 166-169 nitric oxide synthase 3 Homo sapiens 108-112 11397791-3 2001 eNOS Thr(495) was constitutively phosphorylated in porcine aortic endothelial cells and was rapidly dephosphorylated after bradykinin stimulation. Threonine 5-8 nitric oxide synthase 3 Homo sapiens 0-4 11397791-11 2001 These results suggest that the dual phosphorylation of Ser(1177) and Thr(495) determines the activity of eNOS in agonist-stimulated endothelial cells. Threonine 69-72 nitric oxide synthase 3 Homo sapiens 105-109 10671564-2 2000 We demonstrate here that NOS-III, but neither NOS-I nor -II, is rapidly and strongly activated and phosphorylated on both Ser and Thr in the presence of cGMP-dependent protein kinase II (cGK II) and the catalytic subunit of cAMP-dependent protein kinase (cAK) in vitro. Threonine 130-133 nitric oxide synthase 3 Homo sapiens 25-32 33221570-7 2021 Mechanistically, this occurs via the mitochondrial redistribution of uncoupled eNOS secondary to a PKC-dependent phosphorylation of eNOS at Threonine 495 (T495). Threonine 140-149 nitric oxide synthase 3 Homo sapiens 79-83 35617030-7 2022 Mechanistic studies showed that NACT increased inhibitory eNOS phosphorylation of threonine 495 in a ROCK-dependent manner and augmented vascular superoxide and hydrogen peroxide production and NADPH oxidase activity. Threonine 82-91 nitric oxide synthase 3 Homo sapiens 58-62 33578126-7 2021 These effects are dependent on RhoA-ROCK signaling and are mediated via increased phosphorylation of eNOS at Threonine (T)-495. Threonine 109-118 nitric oxide synthase 3 Homo sapiens 101-105 33539947-7 2021 Activation of eNOS in MPO-containing ECs exposed to H2O2 involved a rapid elevation in cytosolic calcium and increased eNOS phosphorylation at Ser-1179 and de-phosphorylation at Thr-497. Threonine 178-181 nitric oxide synthase 3 Homo sapiens 14-18 33539947-8 2021 These signaling events were attenuated by intracellular calcium chelation, removal of extracellular calcium and inhibition of phospholipase C. This study shows that stimulation of endothelial-transcytosed MPO activates eNOS by promoting phospholipase C-dependent calcium signaling and altered eNOS phosphorylation at Ser-1179 and Thr-497. Threonine 330-333 nitric oxide synthase 3 Homo sapiens 219-223 33539947-8 2021 These signaling events were attenuated by intracellular calcium chelation, removal of extracellular calcium and inhibition of phospholipase C. This study shows that stimulation of endothelial-transcytosed MPO activates eNOS by promoting phospholipase C-dependent calcium signaling and altered eNOS phosphorylation at Ser-1179 and Thr-497. Threonine 330-333 nitric oxide synthase 3 Homo sapiens 293-297 9852077-1 1998 Eight polar amino acid residues in the putative substrate-binding region from Thr-360 to Val-379 in human endothelial nitric-oxide synthase (eNOS) (Thr-360, Arg-365, Cys-368, Asp-369, Arg-372, Tyr-373, Glu-377, and Asp-378) were individually mutated. Threonine 78-81 nitric oxide synthase 3 Homo sapiens 106-139 34050207-0 2021 Hypertonic stress modulates eNOS function through O-GlcNAc modification at Thr-866. Threonine 75-78 nitric oxide synthase 3 Homo sapiens 28-32 33221570-7 2021 Mechanistically, this occurs via the mitochondrial redistribution of uncoupled eNOS secondary to a PKC-dependent phosphorylation of eNOS at Threonine 495 (T495). Threonine 140-149 nitric oxide synthase 3 Homo sapiens 132-136 28581447-8 2017 Under basal conditions, adenosine stimulated NO production, eNOS phosphorylation at serine 1177 from 5 minutes to 4 hours and inhibited eNOS phosphorylation at threonine 495 from 5 minutes to 6 hours, but increased phosphorylation of ERK1/2, p38MAPK, and p70S6K only after exposure for 5 minutes. Threonine 160-169 nitric oxide synthase 3 Homo sapiens 136-140 24008236-0 2013 Endothelial nitric oxide synthase phosphorylation at Threonine 495 and mitochondrial reactive oxygen species formation in response to a high H2O2 concentration. Threonine 53-62 nitric oxide synthase 3 Homo sapiens 0-33 27660293-14 2016 Last, silencing CSE by siRNA significantly increased endothelial nitric oxide synthase phosphorylation at threonine 495 levels in human cardiac microvascular endothelial cells. Threonine 106-115 nitric oxide synthase 3 Homo sapiens 53-86 25804308-6 2015 Furthermore, Ang II increased eNOS-Ser(1177) but decreased eNOS-Thr(495), indicating concomitant activation of eNOS. Threonine 64-67 nitric oxide synthase 3 Homo sapiens 59-63 25804308-6 2015 Furthermore, Ang II increased eNOS-Ser(1177) but decreased eNOS-Thr(495), indicating concomitant activation of eNOS. Threonine 64-67 nitric oxide synthase 3 Homo sapiens 59-63 23959544-6 2014 Phosphorylation levels of Ser(1177) and Thr(495) in eNOS were determined after exposure to amlodipine, S(-)-amlodipine, and Ro 31-8220. Threonine 40-43 nitric oxide synthase 3 Homo sapiens 52-56 23959544-10 2014 Western blot assay showed that both amlodipine and Ro31-8220 induced Ser(1177) phosphorylation and weakened Thr(495) phosphorylation in eNOS. Threonine 108-111 nitric oxide synthase 3 Homo sapiens 136-140 23959544-15 2014 It affects eNOS phosphorylation at Ser(1177) and Thr(495) by the PKC pathway, further enhancing eNOS activation. Threonine 49-52 nitric oxide synthase 3 Homo sapiens 96-100 26286023-2 2015 Several mechanisms regulate eNOS activity and function, including phosphorylation at Ser and Thr residues and protein-protein interactions. Threonine 93-96 nitric oxide synthase 3 Homo sapiens 28-32 24573187-4 2014 In addition, apelin-13 dose dependently stimulated the phosphorylation of AMP-activated protein kinase (AMPK) and endothelial nitric oxide synthase (eNOS) at Thr-172 and Ser-1179, respectively. Threonine 158-161 nitric oxide synthase 3 Homo sapiens 114-147 24008236-2 2013 We investigated cellular pathways involved in endothelial nitric oxide synthase (eNOS) phosphorylation at Threonine 495 (Thr(495)) in human umbilical vein endothelial cells (HUVECs) exposed to H2O2. Threonine 106-115 nitric oxide synthase 3 Homo sapiens 46-79 24008236-2 2013 We investigated cellular pathways involved in endothelial nitric oxide synthase (eNOS) phosphorylation at Threonine 495 (Thr(495)) in human umbilical vein endothelial cells (HUVECs) exposed to H2O2. Threonine 106-109 nitric oxide synthase 3 Homo sapiens 46-79 21383022-0 2011 Protein kinase CbetaII-mediated phosphorylation of endothelial nitric oxide synthase threonine 495 mediates the endothelial dysfunction induced by FK506 (tacrolimus). Threonine 85-94 nitric oxide synthase 3 Homo sapiens 51-84 22683691-4 2012 However, the proportion of eNOS which was phosphorylated at serine 1117 and threonine 495 residues was decreased. Threonine 76-85 nitric oxide synthase 3 Homo sapiens 27-31 21757745-11 2011 PAF activated eNOS by increasing phosphorylation of Ser-1177 and inducing dephosphorylation of Thr-495, increasing NO production, and elevating permeability to FITC-dextran 70 in monolayers of cells expressing wild-type and cytosolic eNOS. Threonine 95-98 nitric oxide synthase 3 Homo sapiens 14-18