PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 11721009-0 2001 Molecular dynamics simulations of Gly-12-->Val mutant of p21(ras): dynamic inhibition mechanism. Valine 46-49 H3 histone pseudogene 16 Homo sapiens 60-63 15200054-3 2004 We have now synthesized peptides corresponding to these four domains and find that all of them block Val 12-ras-p21-induced oocyte maturation. Valine 101-104 H3 histone pseudogene 16 Homo sapiens 112-115 9398294-3 1997 The same phenomenon may also account for the diminished GTPase activity of the homologous transforming Gly42 --> Val mutation in p21(ras). Valine 116-119 H3 histone pseudogene 16 Homo sapiens 132-135 9311595-8 1997 These data demonstrate that peptide vaccination with a single mutant p21-ras-derived peptide induces CD4+ and CD8+ CTL specific for nested epitopes, including the Gly --> Val substitution at codon 12, and that both these T-cell sub-sets specifically recognize tumour cells harbouring the corresponding K-ras mutation. Valine 174-177 H3 histone pseudogene 16 Homo sapiens 69-72 1652268-6 1991 When ras proteins were preactivated with the non-hydrolysable GTP analog GppNHp, the time courses of both p21(Gly-12) and p21(Val-12) effects were fast and sustained, suggesting that in intact cells (i) the GDP/GTP exchange is faster for p21(Gly-12) compared to p21(Val-12) and (ii) inactivation of p21(Gly-12) is mediated by GAP-induced GTPase activity. Valine 266-269 H3 histone pseudogene 16 Homo sapiens 106-109 1639630-9 1992 TCC E and F were restricted by HLA-DQ molecules, the former being specific for a mutated p21 ras-derived peptide with Val in position 13 and the latter more broadly reactive. Valine 118-121 H3 histone pseudogene 16 Homo sapiens 89-92 1652268-6 1991 When ras proteins were preactivated with the non-hydrolysable GTP analog GppNHp, the time courses of both p21(Gly-12) and p21(Val-12) effects were fast and sustained, suggesting that in intact cells (i) the GDP/GTP exchange is faster for p21(Gly-12) compared to p21(Val-12) and (ii) inactivation of p21(Gly-12) is mediated by GAP-induced GTPase activity. Valine 126-129 H3 histone pseudogene 16 Homo sapiens 122-125 1652268-6 1991 When ras proteins were preactivated with the non-hydrolysable GTP analog GppNHp, the time courses of both p21(Gly-12) and p21(Val-12) effects were fast and sustained, suggesting that in intact cells (i) the GDP/GTP exchange is faster for p21(Gly-12) compared to p21(Val-12) and (ii) inactivation of p21(Gly-12) is mediated by GAP-induced GTPase activity. Valine 126-129 H3 histone pseudogene 16 Homo sapiens 122-125 1652268-6 1991 When ras proteins were preactivated with the non-hydrolysable GTP analog GppNHp, the time courses of both p21(Gly-12) and p21(Val-12) effects were fast and sustained, suggesting that in intact cells (i) the GDP/GTP exchange is faster for p21(Gly-12) compared to p21(Val-12) and (ii) inactivation of p21(Gly-12) is mediated by GAP-induced GTPase activity. Valine 126-129 H3 histone pseudogene 16 Homo sapiens 122-125 1652268-6 1991 When ras proteins were preactivated with the non-hydrolysable GTP analog GppNHp, the time courses of both p21(Gly-12) and p21(Val-12) effects were fast and sustained, suggesting that in intact cells (i) the GDP/GTP exchange is faster for p21(Gly-12) compared to p21(Val-12) and (ii) inactivation of p21(Gly-12) is mediated by GAP-induced GTPase activity. Valine 126-129 H3 histone pseudogene 16 Homo sapiens 122-125 9021406-5 1997 C27 also exhibited a much stronger response to a mutated p21 Ras whole-protein molecule-carrying Val at position 12, as compared with the wild-type protein. Valine 97-100 H3 histone pseudogene 16 Homo sapiens 57-60 8747433-1 1995 Molecular dynamics calculations have been performed to determine the average structures of ras-gene-encoded p21 proteins bound to GTP, i.e., the normal (wild-type) protein and two oncogenic forms of this protein, the Val 12- and Leu 61-p21 proteins. Valine 217-220 H3 histone pseudogene 16 Homo sapiens 108-111 1652268-6 1991 When ras proteins were preactivated with the non-hydrolysable GTP analog GppNHp, the time courses of both p21(Gly-12) and p21(Val-12) effects were fast and sustained, suggesting that in intact cells (i) the GDP/GTP exchange is faster for p21(Gly-12) compared to p21(Val-12) and (ii) inactivation of p21(Gly-12) is mediated by GAP-induced GTPase activity. Valine 266-269 H3 histone pseudogene 16 Homo sapiens 122-125 1652268-6 1991 When ras proteins were preactivated with the non-hydrolysable GTP analog GppNHp, the time courses of both p21(Gly-12) and p21(Val-12) effects were fast and sustained, suggesting that in intact cells (i) the GDP/GTP exchange is faster for p21(Gly-12) compared to p21(Val-12) and (ii) inactivation of p21(Gly-12) is mediated by GAP-induced GTPase activity. Valine 266-269 H3 histone pseudogene 16 Homo sapiens 122-125 1652268-6 1991 When ras proteins were preactivated with the non-hydrolysable GTP analog GppNHp, the time courses of both p21(Gly-12) and p21(Val-12) effects were fast and sustained, suggesting that in intact cells (i) the GDP/GTP exchange is faster for p21(Gly-12) compared to p21(Val-12) and (ii) inactivation of p21(Gly-12) is mediated by GAP-induced GTPase activity. Valine 266-269 H3 histone pseudogene 16 Homo sapiens 122-125 1652268-6 1991 When ras proteins were preactivated with the non-hydrolysable GTP analog GppNHp, the time courses of both p21(Gly-12) and p21(Val-12) effects were fast and sustained, suggesting that in intact cells (i) the GDP/GTP exchange is faster for p21(Gly-12) compared to p21(Val-12) and (ii) inactivation of p21(Gly-12) is mediated by GAP-induced GTPase activity. Valine 266-269 H3 histone pseudogene 16 Homo sapiens 122-125 2014702-1 1991 A hypothesis presented in the paper discusses the role of valine substitution in p21 protein of H-ras, a product of ras family oncogene. Valine 58-64 H3 histone pseudogene 16 Homo sapiens 81-84 2014702-5 1991 Based on similarity of valine substitution in p21 protein and hemoglobin, a relationship between certain forms of malignant transformation and malaria at molecular level is suggested. Valine 23-29 H3 histone pseudogene 16 Homo sapiens 46-49 2682005-7 1989 Glycine----arginine mutation and glycine----valine mutation of codon 12 in ras p21 was observed in 40 and 31% of colon cancers, respectively. Valine 44-50 H3 histone pseudogene 16 Homo sapiens 79-82 2483936-3 1989 The mRNA expression of the transgenes was detected in two tumors obtained after introduction of the DNA fragment containing the activated human c-Ha-ras gene for p21 with valine at the 12th codon or with leucine at the 61st codon. Valine 171-177 H3 histone pseudogene 16 Homo sapiens 162-165 2508560-8 1989 This mutation is theoretically equivalent to reversion of the Gly to Val transforming mutation of the cellular form of the ras gene product p21, a protein proposed to be structurally similar to EF-Tu in the GDP binding domain. Valine 69-72 H3 histone pseudogene 16 Homo sapiens 140-143 2785386-4 1989 Results suggest that restriction of conformational adaptation may contribute to the transforming capacity of the Val-12 p21 protein. Valine 113-116 H3 histone pseudogene 16 Homo sapiens 120-123 2686707-10 1989 This segment contains a Val residue where a Gly occurs in the p21 protein. Valine 24-27 H3 histone pseudogene 16 Homo sapiens 62-65 2686707-12 1989 If these structures that are superimposable on EFtu are introduced into the p21 protein structure, bad contacts occur between the sidechain of the residue (here Val) at position 12 and another phosphate binding loop region around position 61. Valine 161-164 H3 histone pseudogene 16 Homo sapiens 76-79 2669815-2 1989 For example, the P21 proteins with Arg 12 or Val 13 are both known to be actively transforming. Valine 45-48 H3 histone pseudogene 16 Homo sapiens 17-20 2642607-2 1989 To understand the structural reasons behind cell transformation arising from this single amino acid substitution, we have determined the crystal structure of the GDP-bound form of the mutant protein, p21(Val-12), encoded by this oncogene. Valine 204-207 H3 histone pseudogene 16 Homo sapiens 200-203 2642607-3 1989 We report here the overall structure of p21(Val-12) at 2.2 A resolution and compare it with the structure of the normal c-Ha-ras protein. Valine 44-47 H3 histone pseudogene 16 Homo sapiens 40-43 2567085-6 1989 Since one would anticipate that the valine/cysteine substitution at position 12 of the ras p21 would occur at only low frequencies in human tumors, our results with DWP are consistent with this hypothesis. Valine 36-42 H3 histone pseudogene 16 Homo sapiens 91-94 3082814-1 1986 A 576 base-pair DNA encoding human c-Ha-ras protein (p21) with a valine codon at position 12 has been synthesized by ligation of chemically synthesized oligodeoxyribonucleotides. Valine 65-71 H3 histone pseudogene 16 Homo sapiens 53-56 3277185-4 1988 However, substitution of variant amino acids from the 80 C-terminal residues (amino acids 138-218) of R-ras p23 for the corresponding p21 amino acids (residues 112-189) inactivated the transforming activity of position-12 valine-substituted p21. Valine 222-228 H3 histone pseudogene 16 Homo sapiens 134-137 3277185-4 1988 However, substitution of variant amino acids from the 80 C-terminal residues (amino acids 138-218) of R-ras p23 for the corresponding p21 amino acids (residues 112-189) inactivated the transforming activity of position-12 valine-substituted p21. Valine 222-228 H3 histone pseudogene 16 Homo sapiens 241-244 3313005-10 1987 R-ras p21-like proteins, made by eliminating the first 26 R-ras codons, displayed evident mobility differences between the pro form and mature form, along with a valine 12 substitution-dependent change in electrophoretic mobility. Valine 162-168 H3 histone pseudogene 16 Homo sapiens 6-9 3332015-4 1986 One of the two tumors thus obtained was formed after the injection of 6.3 kb DNA fragment containing the gene for p21 with valine at the twelfth position. Valine 123-129 H3 histone pseudogene 16 Homo sapiens 114-117 7133135-2 1982 This substitution results in the incorporation of valine instead of glycine as the twelfth amino acid residue of the T24 oncogene-encoded p21 protein. Valine 50-56 H3 histone pseudogene 16 Homo sapiens 138-141 3919305-3 1985 Recently, we and others have observed that normal p21, encoded by the Ha-ras gene, has a GTP hydrolytic activity that is reduced by the oncogenic substitutions Val 12 or Thr 59. Valine 160-163 H3 histone pseudogene 16 Homo sapiens 50-53 6308640-3 1983 These findings predicted that the resulting oncogene would code for a structurally altered p21 protein containing valine instead of glycine as its 12th amino acid residue. Valine 114-120 H3 histone pseudogene 16 Homo sapiens 91-94 6308640-8 1983 Computer analysis of the secondary structure of c-has/bas encoded p21 proteins indicates that substitution of the glycine residue located at position 12, not only by aspartic acid or valine but also by any other amino acid, would result in the same structural alteration. Valine 183-189 H3 histone pseudogene 16 Homo sapiens 66-69