PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 2426695-5 1986 The estradiol receptor was able to bind to aprotinin-agarose only under transforming conditions; i.e., the interaction was hormone- and temperature-dependent and inhibited by molybdate. molybdate 175-184 estrogen receptor 1 Homo sapiens 4-22 11795466-5 2001 The GST.90-ER interaction was consistently reduced in the presence of increasing concentrations of potassium chloride or when cytosolic ER-Hsp90 complexes were previously stabilized by molybdate, indicating that GST.90-ER complexes behave like cytosolic Hsp90-ER complexes. molybdate 185-194 estrogen receptor 1 Homo sapiens 11-13 3527482-4 1986 Estrogen and molybdate substantially stabilized estrogen receptor during the assay, but improper handling of tissue before the assay may cause similar, substantial decreases in estrogen receptor by either method. molybdate 13-22 estrogen receptor 1 Homo sapiens 48-65 7476980-3 1995 Using MCF-7 human breast cancer cell extracts and circular permutation analysis, it was determined that molybdate-stabilized, unoccupied cytosolic ER was unable to bind to ERE-containing DNA fragments when maintained at 4 C, but that thermal activation enabled the cytosolic receptor to bind and bend ERE-containing DNA fragments to the same extent as ER present in whole cell extracts. molybdate 104-113 estrogen receptor 1 Homo sapiens 147-149 1944301-9 1991 The binding of molybdate-stabilized nonactivated 8S ER to VitA2ERE was studied to determine the effect of temperature on ER binding. molybdate 15-24 estrogen receptor 1 Homo sapiens 52-54 1944301-9 1991 The binding of molybdate-stabilized nonactivated 8S ER to VitA2ERE was studied to determine the effect of temperature on ER binding. molybdate 15-24 estrogen receptor 1 Homo sapiens 63-65 3571221-0 1987 Transformation of the 8-9 S molybdate-stabilized estrogen receptor from low-affinity to high-affinity state without dissociation into subunits. molybdate 28-37 estrogen receptor 1 Homo sapiens 49-66 4077842-0 1985 Effect of molybdate on the molecular organization of the estrogen receptor system of porcine uterus. molybdate 10-19 estrogen receptor 1 Homo sapiens 57-74 4077842-1 1985 Molybdate was shown to have complex effects in modulating the molecular organization of the constituents of the estrogen receptor (ER) system of porcine uterus. molybdate 0-9 estrogen receptor 1 Homo sapiens 112-129 4077842-1 1985 Molybdate was shown to have complex effects in modulating the molecular organization of the constituents of the estrogen receptor (ER) system of porcine uterus. molybdate 0-9 estrogen receptor 1 Homo sapiens 131-133 4077842-5 1985 Molybdate regulates the specific interaction of vero-ER with ERBFs in a complex way. molybdate 0-9 estrogen receptor 1 Homo sapiens 53-55 4077842-6 1985 The apparent Kd value (6.7 X 10(-10) M) of vero-ER with "8S" ER-FF in the presence of molybdate (30 mM) was decreased remarkably as compared with that (2.7 X 10(-9) M) in the absence of molybdate. molybdate 86-95 estrogen receptor 1 Homo sapiens 48-50 4077842-6 1985 The apparent Kd value (6.7 X 10(-10) M) of vero-ER with "8S" ER-FF in the presence of molybdate (30 mM) was decreased remarkably as compared with that (2.7 X 10(-9) M) in the absence of molybdate. molybdate 186-195 estrogen receptor 1 Homo sapiens 48-50 4077842-7 1985 In contrast, the apparent Kd value (3.7 X 10(-9) M) of vero-ER with "5S" ER-FF observed in the presence of molybdate (30 mM) was increased over ten-fold as compared with that in the absence of molybdate. molybdate 107-116 estrogen receptor 1 Homo sapiens 60-62 4077842-7 1985 In contrast, the apparent Kd value (3.7 X 10(-9) M) of vero-ER with "5S" ER-FF observed in the presence of molybdate (30 mM) was increased over ten-fold as compared with that in the absence of molybdate. molybdate 193-202 estrogen receptor 1 Homo sapiens 60-62 4077842-8 1985 Meanwhile, the affinity (Kd, 5 X 10(-9) M) of vero-ER for "6S" ER-FF was scarcely influenced by molybdate. molybdate 96-105 estrogen receptor 1 Homo sapiens 51-53 4077842-9 1985 These results reveal the mechanism by which molybdate selectively stabilizes "8S" ER. molybdate 44-53 estrogen receptor 1 Homo sapiens 82-84 3980464-7 1985 Starting from unfractionated cytosol containing molybdate-stabilized nontransformed 8S estrogen receptor with estradiol 7 alpha-(CH2)10-CO-NH-(CH2)2-O-(CH2)2-O-(CH2)2-NH-CO-(CH2)3-NH-biotin, preliminary experiments using avidin-agarose chromatography and then a specific elution step by exchange with free [3H]estradiol, allowed a 500-1,500-fold purification. molybdate 48-57 estrogen receptor 1 Homo sapiens 87-104 3988760-7 1985 These findings show that the molybdate-stabilized estrogen receptor exists in a single discrete form, but otherwise exhibits multiple forms that are probably artifactual. molybdate 29-38 estrogen receptor 1 Homo sapiens 50-67 6697008-0 1984 The effect of molybdate on the intracellular distribution of estrogen receptor in mammary tumors. molybdate 14-23 estrogen receptor 1 Homo sapiens 61-78 6697008-7 1984 Estrogen receptor-negative tumors however yield fewer nuclei, particularly when they are processed in the presence of molybdate. molybdate 118-127 estrogen receptor 1 Homo sapiens 0-17 6697008-8 1984 We conclude that when homogenization of tumors is carried out in the presence of molybdate, a significant fraction of estrogen receptor which would normally be present in purified nuclei is lost, perhaps by leakage through the nuclear membrane. molybdate 81-90 estrogen receptor 1 Homo sapiens 118-135 6745845-6 1984 Of the 8 tumors for which receptor values in the presence of molybdate (M+) and its absence (M-) can be compared, detectable ERc of 6 and PRc of 7 tumors increased with molybdate, and ERc of 2 and PRc of 1 tumor showed no change. molybdate 169-178 estrogen receptor 1 Homo sapiens 125-128