PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 807304-5 1975 Three factors have contributed to the subsequent resolution of the Z-fraction and partial characterization of that protein within the fraction with ligand-binding properties (Z-protein): (1) the use of hexachlorophene as ligand; (2) the inclusion of glycerol, 20%, during isolation to prevent aggregation and loss of binding-activity; and (3) the development of a charcoal binding assay. Hexachlorophene 202-217 fatty acid binding protein 1 Rattus norvegicus 175-184 807304-11 1975 With use of the charcoal method, apparent dissociation constants for the interaction between Z-protein and hexachlorophene, bilirubin and L-thyroxine, were found to be 20, 50, and 350 muM, respectively. Hexachlorophene 107-122 fatty acid binding protein 1 Rattus norvegicus 93-102