PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 20185827-6 2010 Finally, we found that OGG1, NTH1, and REF1/APE1 each contribute to the BRCA1 protection against oxidative stress due to hydrogen peroxide and that hydrogen peroxide stimulates the expression of BRCA1 and the three BER enzymes. Hydrogen Peroxide 121-138 apurinic/apyrimidinic endodeoxyribonuclease 1 Homo sapiens 39-43 20185827-6 2010 Finally, we found that OGG1, NTH1, and REF1/APE1 each contribute to the BRCA1 protection against oxidative stress due to hydrogen peroxide and that hydrogen peroxide stimulates the expression of BRCA1 and the three BER enzymes. Hydrogen Peroxide 121-138 apurinic/apyrimidinic endodeoxyribonuclease 1 Homo sapiens 44-48 20054488-4 2009 Also the overexpression of Ref-1 significantly suppressed Pb-induced superoxide and hydrogen peroxide elevation in the endothelial cells. Hydrogen Peroxide 84-101 apurinic/apyrimidinic endodeoxyribonuclease 1 Homo sapiens 27-32 19060414-0 2008 Silencing of Ref-1 expression by retrovirus-mediated shRNA sensitizes HEK293 cells to hydrogen peroxide-induced apoptosis. Hydrogen Peroxide 86-103 apurinic/apyrimidinic endodeoxyribonuclease 1 Homo sapiens 13-18 19567405-5 2009 In cells treated with H(2)O(2), RECQL4 co-localizes with APE1, and FEN1, key participants in base excision repair. Hydrogen Peroxide 22-30 apurinic/apyrimidinic endodeoxyribonuclease 1 Homo sapiens 57-61 19060414-3 2008 Our results showed that downregulation of Ref-1 rendered cells more sensitive to H(2)O(2)-induced apoptosis. Hydrogen Peroxide 81-89 apurinic/apyrimidinic endodeoxyribonuclease 1 Homo sapiens 42-47 17443679-5 2007 Interestingly, GD cells are more sensitive to H(2)O(2) induced cell death, suggesting a dysregulation in the adaptive response to oxidative stress in which APE1/Ref-1 plays a central role. Hydrogen Peroxide 46-54 apurinic/apyrimidinic endodeoxyribonuclease 1 Homo sapiens 156-160 18061304-9 2008 In the lung adenocarcinoma cell line, H460, treatment with hydrogen peroxide in the presence of a catalase inhibitor, aminotriazole, increased APE1/ref-1 expression, suggesting oxidative stress might have contributed to the induction of APE1/ref-1. Hydrogen Peroxide 59-76 apurinic/apyrimidinic endodeoxyribonuclease 1 Homo sapiens 143-147 18061304-9 2008 In the lung adenocarcinoma cell line, H460, treatment with hydrogen peroxide in the presence of a catalase inhibitor, aminotriazole, increased APE1/ref-1 expression, suggesting oxidative stress might have contributed to the induction of APE1/ref-1. Hydrogen Peroxide 59-76 apurinic/apyrimidinic endodeoxyribonuclease 1 Homo sapiens 148-153 18061304-9 2008 In the lung adenocarcinoma cell line, H460, treatment with hydrogen peroxide in the presence of a catalase inhibitor, aminotriazole, increased APE1/ref-1 expression, suggesting oxidative stress might have contributed to the induction of APE1/ref-1. Hydrogen Peroxide 59-76 apurinic/apyrimidinic endodeoxyribonuclease 1 Homo sapiens 237-241 18061304-9 2008 In the lung adenocarcinoma cell line, H460, treatment with hydrogen peroxide in the presence of a catalase inhibitor, aminotriazole, increased APE1/ref-1 expression, suggesting oxidative stress might have contributed to the induction of APE1/ref-1. Hydrogen Peroxide 59-76 apurinic/apyrimidinic endodeoxyribonuclease 1 Homo sapiens 242-247 18324520-7 2008 It was found that exposure of HeLa cells to H(2)O(2) and to histone deacetylase inhibitors increases acetylation of APE1 and induction of PTEN. Hydrogen Peroxide 44-52 apurinic/apyrimidinic endodeoxyribonuclease 1 Homo sapiens 116-120 17566060-8 2007 Ref-1 depletion also inhibited 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced anchorage-independent growth of JB6P+ by 40% and reduced the colony numbers of JB6P+/H2O2 and JB6P+/Cd cells. Hydrogen Peroxide 167-171 apurinic/apyrimidinic endodeoxyribonuclease 1 Homo sapiens 0-5 17566060-9 2007 Mechanistic studies revealed that Ref-1 reduction was associated with an increase of intracellular ROS levels and a marked decrease of activator protein-1 (AP-1) transcription activities in JB6P+/H2O2 cells. Hydrogen Peroxide 196-200 apurinic/apyrimidinic endodeoxyribonuclease 1 Homo sapiens 34-39 17443679-5 2007 Interestingly, GD cells are more sensitive to H(2)O(2) induced cell death, suggesting a dysregulation in the adaptive response to oxidative stress in which APE1/Ref-1 plays a central role. Hydrogen Peroxide 46-54 apurinic/apyrimidinic endodeoxyribonuclease 1 Homo sapiens 161-166 16730871-6 2006 Cells transiently transfected with the wild-type APE1 decreased the PARP1 activation after H2O2 treatment, while such suppression did not occur with the expression of the R177A APE1 mutant. Hydrogen Peroxide 91-95 apurinic/apyrimidinic endodeoxyribonuclease 1 Homo sapiens 49-53 16399009-15 2006 An increase in endogenous H2O2 level during senescence may be the result of a programmed down-regulation of APX enzyme activity, which seems to be the prerequisite factor for initiating senescence process in gladiolus tepal. Hydrogen Peroxide 26-30 apurinic/apyrimidinic endodeoxyribonuclease 1 Homo sapiens 108-111 10666449-7 2000 Exposure of B lymphocytes to H(2)O(2)induced a rapid and sustained increase in Ref-1 protein levels in the nucleus as evaluated by both western blot analysis and by pulse-chase experiments. Hydrogen Peroxide 29-37 apurinic/apyrimidinic endodeoxyribonuclease 1 Homo sapiens 79-84 16373707-5 2005 Ref-1 overexpression also protected melanoma cells from cisplatin- or H2O2-induced apoptosis, whereas increased apoptosis was observed with Ref-1 antisense construct infection. Hydrogen Peroxide 70-74 apurinic/apyrimidinic endodeoxyribonuclease 1 Homo sapiens 0-5 16077024-8 2005 The biological relevance of our data is reinforced by the observation that APE1/Ref-1 stimulation by ATP protects ARO cells by H2O2-induced cell death. Hydrogen Peroxide 127-131 apurinic/apyrimidinic endodeoxyribonuclease 1 Homo sapiens 75-79 16077024-8 2005 The biological relevance of our data is reinforced by the observation that APE1/Ref-1 stimulation by ATP protects ARO cells by H2O2-induced cell death. Hydrogen Peroxide 127-131 apurinic/apyrimidinic endodeoxyribonuclease 1 Homo sapiens 80-85 15661660-6 2005 The reduction in Ape1 significantly reduces cell viability in cultures 24 h after a 1-h exposure to 25-300 microM H2O2, compared to SCsiRNA treated controls. Hydrogen Peroxide 114-118 apurinic/apyrimidinic endodeoxyribonuclease 1 Homo sapiens 17-21 15661660-8 2005 Reduced Ape1 levels also increase caspase-3 activity in the cells, 2-3-fold, 60min after a 1-h exposure to 100 microM H2O2 in the cultures. Hydrogen Peroxide 118-122 apurinic/apyrimidinic endodeoxyribonuclease 1 Homo sapiens 8-12 15661660-9 2005 Exposing neuronal cultures with reduced expression of Ape1 to 65 microM H2O2 (hippocampal) or 300 microM H2O2 (sensory) for 1h results in a 3-fold and 1.5-fold increase in the phosphorylation of histone H2A.X compared to cells exposed to SCsiRNA. Hydrogen Peroxide 72-76 apurinic/apyrimidinic endodeoxyribonuclease 1 Homo sapiens 54-58 15661660-9 2005 Exposing neuronal cultures with reduced expression of Ape1 to 65 microM H2O2 (hippocampal) or 300 microM H2O2 (sensory) for 1h results in a 3-fold and 1.5-fold increase in the phosphorylation of histone H2A.X compared to cells exposed to SCsiRNA. Hydrogen Peroxide 105-109 apurinic/apyrimidinic endodeoxyribonuclease 1 Homo sapiens 54-58 15661660-10 2005 Overexpressing wild-type Ape1 in hippocampal and sensory cells using adenoviral expression constructs results in significant increase in cell viability after exposure to various concentrations of H2O2. Hydrogen Peroxide 196-200 apurinic/apyrimidinic endodeoxyribonuclease 1 Homo sapiens 25-29 12384995-0 2002 H(2)O(2) induces translocation of APE/Ref-1 to mitochondria in the Raji B-cell line. Hydrogen Peroxide 0-8 apurinic/apyrimidinic endodeoxyribonuclease 1 Homo sapiens 34-37 12384995-0 2002 H(2)O(2) induces translocation of APE/Ref-1 to mitochondria in the Raji B-cell line. Hydrogen Peroxide 0-8 apurinic/apyrimidinic endodeoxyribonuclease 1 Homo sapiens 38-43 15362040-0 2004 Helicobacter pylori and H2O2 increase AP endonuclease-1/redox factor-1 expression in human gastric epithelial cells. Hydrogen Peroxide 24-28 apurinic/apyrimidinic endodeoxyribonuclease 1 Homo sapiens 38-70 26268226-7 2015 RESULTS: Bcl2, an anti-apoptotic molecule and oncoprotein, effectively inhibits the endonuclease activity of mitochondrial APE1 (mtAPE1), leading to significant retardation of mtDNA repair and enhanced frequency of mtDNA mutations following exposure of cells to hydrogen peroxide (H2O2) or nitrosamine 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK, a carcinogen in cigarette smoke). Hydrogen Peroxide 262-279 apurinic/apyrimidinic endodeoxyribonuclease 1 Homo sapiens 123-127 9766671-7 1998 To further prove the involvement of APE in adaptation against induced chromosomal breakage, we transfected human APE cDNA driven by an inducible promoter into CHO cells and observed that transient induction of APE reduced the clastogenic effect of H2O2. Hydrogen Peroxide 248-252 apurinic/apyrimidinic endodeoxyribonuclease 1 Homo sapiens 113-116 33679014-6 2021 H2O2 treatment also protected the sensitive cultivars from drought stress by increasing CAT, POX, APX, MDHAR and GR enzymes. Hydrogen Peroxide 0-4 apurinic/apyrimidinic endodeoxyribonuclease 1 Homo sapiens 98-101 32627281-8 2020 Longer exposure to H2 O2 resulted in extensive damage to DNA that could not be repaired, probably due to suboptimal induction of APE1, an enzyme necessary for base excision repair (BER). Hydrogen Peroxide 19-24 apurinic/apyrimidinic endodeoxyribonuclease 1 Homo sapiens 129-133 29230937-5 2018 Additionally, the transgenic plants reduced H2 O2 and O2 - accumulation under salinity, which could be due to up-regulation of ROS scavenger activities such as SOD, APX and CAT as well as CmHSP70, CmHSP90. Hydrogen Peroxide 44-49 apurinic/apyrimidinic endodeoxyribonuclease 1 Homo sapiens 165-168 10441516-3 1999 Recently, it was shown that the level of APE mRNA and protein is enhanced upon treatment of cells with oxidative agents, such as hydrogen peroxide (H(2)O(2)), which gives rise to an adaptive response of cells to oxidative stress. Hydrogen Peroxide 129-146 apurinic/apyrimidinic endodeoxyribonuclease 1 Homo sapiens 41-44 8592168-3 1995 The cell lines having lower APEX expression showed higher sensitivity to MMS and H2O2 which are known to induce AP sites and single strand breaks on DNA, respectively. Hydrogen Peroxide 81-85 apurinic/apyrimidinic endodeoxyribonuclease 1 Homo sapiens 28-32 33410354-13 2021 Prolonged hypoxia and the addition of H2O2 induced the expression of Ref-1. Hydrogen Peroxide 38-42 apurinic/apyrimidinic endodeoxyribonuclease 1 Homo sapiens 69-74 33065487-4 2020 Thus, we aimed to investigate whether the silencing of APE1 by shRNA can interfere with the survival of AGS gastric cancer cells after treatment with hydrogen peroxide (H2O2) and/or H. pylori extract (HPE) and its relation with the expression of DDR genes (ATM, ATR, and H2AX) and miRNAs that target DDR genes. Hydrogen Peroxide 150-167 apurinic/apyrimidinic endodeoxyribonuclease 1 Homo sapiens 55-59 33065487-4 2020 Thus, we aimed to investigate whether the silencing of APE1 by shRNA can interfere with the survival of AGS gastric cancer cells after treatment with hydrogen peroxide (H2O2) and/or H. pylori extract (HPE) and its relation with the expression of DDR genes (ATM, ATR, and H2AX) and miRNAs that target DDR genes. Hydrogen Peroxide 169-173 apurinic/apyrimidinic endodeoxyribonuclease 1 Homo sapiens 55-59 33065487-5 2020 In the AGS cells expressing APE1, isolated or combined treatment with H2O2 and HPE promoted a slight increase in the cell proliferation and increased the levels of intracellular ROS and DNA double strand breaks (DSBs) indicated by H2AX foci, a reduction in the proportion of cells in the G0/G1 phase and an increase in the initial apoptosis rate. Hydrogen Peroxide 70-74 apurinic/apyrimidinic endodeoxyribonuclease 1 Homo sapiens 28-32 26268226-7 2015 RESULTS: Bcl2, an anti-apoptotic molecule and oncoprotein, effectively inhibits the endonuclease activity of mitochondrial APE1 (mtAPE1), leading to significant retardation of mtDNA repair and enhanced frequency of mtDNA mutations following exposure of cells to hydrogen peroxide (H2O2) or nitrosamine 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK, a carcinogen in cigarette smoke). Hydrogen Peroxide 281-285 apurinic/apyrimidinic endodeoxyribonuclease 1 Homo sapiens 123-127 23369758-6 2013 In addition, we found that the APE1-148Glu variant increased the 8-OHdG levels of cultured human melanocytes treated with H2O2, without any impact on the endonuclease activity. Hydrogen Peroxide 122-126 apurinic/apyrimidinic endodeoxyribonuclease 1 Homo sapiens 31-35 26081414-7 2015 Moreover, treatment of pancreatic cancer cells with H2O2 to induce oxidative stress resulted in upregulated ROS levels, decreased Ape1 at both the mRNA and protein level, and alterations in WNT/beta-catenin pathway components. Hydrogen Peroxide 52-56 apurinic/apyrimidinic endodeoxyribonuclease 1 Homo sapiens 130-134 26271980-1 2015 OBJECTIVE: To investigate the effects of Astragalus polysaccharide (APS) on the expressions of apurinic/apyrimidinic endonuclease/redox factor-1 (APE/Ref-1) and thioredoxin (TRX) in MRC-5 human embryo lung fibroblasts induced by hydrogen peroxide (H2O2) and explore the mechanism of APS protecting MRC-5 cells from oxidative damage. Hydrogen Peroxide 229-246 apurinic/apyrimidinic endodeoxyribonuclease 1 Homo sapiens 146-149 26271980-1 2015 OBJECTIVE: To investigate the effects of Astragalus polysaccharide (APS) on the expressions of apurinic/apyrimidinic endonuclease/redox factor-1 (APE/Ref-1) and thioredoxin (TRX) in MRC-5 human embryo lung fibroblasts induced by hydrogen peroxide (H2O2) and explore the mechanism of APS protecting MRC-5 cells from oxidative damage. Hydrogen Peroxide 248-252 apurinic/apyrimidinic endodeoxyribonuclease 1 Homo sapiens 146-149 22148505-7 2012 Within 5 min of exposure to hydrogen peroxide, a single disulfide bond formed between C65 and C138 followed by the formation of three additional disulfide bonds within 15 min; 10 total disulfide bonds formed within 1 h. A single mixed-disulfide bond involving C99 of APE1 was observed for the reaction of oxidized APE1 with thioredoxin (TRX). Hydrogen Peroxide 28-45 apurinic/apyrimidinic endodeoxyribonuclease 1 Homo sapiens 267-271 22841870-5 2012 Here, we report that in cultured primary neurons, diminution of cellular ATP by either oligomycin or H(2)O(2) is accompanied by depletion of nuclear Ape1, while other BER proteins are unaffected and retain their nuclear localization under these conditions. Hydrogen Peroxide 101-109 apurinic/apyrimidinic endodeoxyribonuclease 1 Homo sapiens 149-153 22709585-6 2012 Localization experiments show that APE1 increases in the mitochondria of wild-type Q7 cells but not in the mutant huntingtin Q111 cells after treatment with hydrogen peroxide. Hydrogen Peroxide 157-174 apurinic/apyrimidinic endodeoxyribonuclease 1 Homo sapiens 35-39 22877972-7 2012 The oxidative stress generated with 750 muM H2O2 caused two times greater damages in mtDNA compared to nDNA, which included the nonexpressed beta-globin region (1.507+-0.110 lesions/10 kb) and the expressed APEX1 gene (1.623+-0.243 lesions/10 kb) with respect to the control region. Hydrogen Peroxide 44-48 apurinic/apyrimidinic endodeoxyribonuclease 1 Homo sapiens 207-212 22148505-7 2012 Within 5 min of exposure to hydrogen peroxide, a single disulfide bond formed between C65 and C138 followed by the formation of three additional disulfide bonds within 15 min; 10 total disulfide bonds formed within 1 h. A single mixed-disulfide bond involving C99 of APE1 was observed for the reaction of oxidized APE1 with thioredoxin (TRX). Hydrogen Peroxide 28-45 apurinic/apyrimidinic endodeoxyribonuclease 1 Homo sapiens 314-318