PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 15231710-5 2004 Moreover, Western blot experiments indicated that when Egr-1 expression was up-regulated by treating FRTL-5 cells with H2O2, an increase in PTEN expression was also observed. Hydrogen Peroxide 119-123 early growth response 1 Rattus norvegicus 55-60 30939252-10 2019 These data show that H2O2 enhances the expression of Egr-1, which was associated with increased phosphorylation of Akt, and H2O2 triggers its effects on Egr-1 expression through c-Src-mediated Akt and CREB-dependent signaling events in VSMCs. Hydrogen Peroxide 124-128 early growth response 1 Rattus norvegicus 153-158 19681663-0 2010 ERKs and JNKs mediate hydrogen peroxide-induced Egr-1 expression and nuclear accumulation in H9c2 cells. Hydrogen Peroxide 22-39 early growth response 1 Rattus norvegicus 48-53 19681663-4 2010 Egr-1 mRNA levels were found to be maximally induced after 2 h in H2O2-treated H9c2 cells. Hydrogen Peroxide 66-70 early growth response 1 Rattus norvegicus 0-5 19681663-5 2010 Egr-1 respective response at the protein level, was found to be maximally induced after 2 h of treatment with 200 microM H2O2, remaining elevated for 6 h, and declining thereafter. Hydrogen Peroxide 121-125 early growth response 1 Rattus norvegicus 0-5 19681663-6 2010 H2O2-induced upregulation of Egr-1 mRNA and protein levels was ablated in the presence of agents inhibiting ERKs pathway (PD98059) and JNKs (SP600125, AS601245). Hydrogen Peroxide 0-4 early growth response 1 Rattus norvegicus 29-34 19681663-7 2010 Immunofluorescent experiments revealed H2O2-induced Egr-1 nuclear sequestration to be also ERK- and JNK-dependent. Hydrogen Peroxide 39-43 early growth response 1 Rattus norvegicus 52-57 19681663-8 2010 Overall, our results show for the first time the fundamental role of ERKs and JNKs in regulating Egr-1 response to H2O2 treatment in cardiac cells at multiple levels: mRNA, protein and subcellular distribution. Hydrogen Peroxide 115-119 early growth response 1 Rattus norvegicus 97-102 30939252-4 2019 Recent studies have shown that the reactive oxygen species hydrogen peroxide (H2O2) increases Egr-1 expression in VSMCs; however, signaling events leading to H2O2-induced Egr-1 expression are not fully understood. Hydrogen Peroxide 78-82 early growth response 1 Rattus norvegicus 94-99 30939252-4 2019 Recent studies have shown that the reactive oxygen species hydrogen peroxide (H2O2) increases Egr-1 expression in VSMCs; however, signaling events leading to H2O2-induced Egr-1 expression are not fully understood. Hydrogen Peroxide 78-82 early growth response 1 Rattus norvegicus 171-176 30939252-4 2019 Recent studies have shown that the reactive oxygen species hydrogen peroxide (H2O2) increases Egr-1 expression in VSMCs; however, signaling events leading to H2O2-induced Egr-1 expression are not fully understood. Hydrogen Peroxide 158-162 early growth response 1 Rattus norvegicus 94-99 30939252-4 2019 Recent studies have shown that the reactive oxygen species hydrogen peroxide (H2O2) increases Egr-1 expression in VSMCs; however, signaling events leading to H2O2-induced Egr-1 expression are not fully understood. Hydrogen Peroxide 158-162 early growth response 1 Rattus norvegicus 171-176 30939252-5 2019 Therefore, we aimed to determine the signaling pathways implicated in H2O2-induced Egr-1 expression in rat VSMCs. Hydrogen Peroxide 70-74 early growth response 1 Rattus norvegicus 83-88 30939252-6 2019 Pharmacological blockade of the phosphatidylinositol 3-kinase/Akt pathway by wortmannin or SC66 significantly inhibited the protein and mRNA levels of Egr-1 induced by H2O2. Hydrogen Peroxide 168-172 early growth response 1 Rattus norvegicus 151-156 30939252-7 2019 H2O2-induced Egr-1 expression was associated with increased phosphorylation of cyclic AMP response element-binding (CREB) protein, and pharmacological inhibition or silencing of Akt attenuated both H2O2-induced CREB phosphorylation and Egr-1 expression. Hydrogen Peroxide 0-4 early growth response 1 Rattus norvegicus 13-18 30939252-7 2019 H2O2-induced Egr-1 expression was associated with increased phosphorylation of cyclic AMP response element-binding (CREB) protein, and pharmacological inhibition or silencing of Akt attenuated both H2O2-induced CREB phosphorylation and Egr-1 expression. Hydrogen Peroxide 0-4 early growth response 1 Rattus norvegicus 236-241 30939252-7 2019 H2O2-induced Egr-1 expression was associated with increased phosphorylation of cyclic AMP response element-binding (CREB) protein, and pharmacological inhibition or silencing of Akt attenuated both H2O2-induced CREB phosphorylation and Egr-1 expression. Hydrogen Peroxide 198-202 early growth response 1 Rattus norvegicus 13-18 30939252-8 2019 Moreover, RNA interference-mediated depletion of CREB almost completely suppressed the stimulatory effect of H2O2 on Egr-1 expression. Hydrogen Peroxide 109-113 early growth response 1 Rattus norvegicus 117-122 30939252-9 2019 Pharmacological blockade or silencing of c-Src resulted in significant suppression of H2O2-induced Egr-1 expression as well as Akt and CREB phosphorylation. Hydrogen Peroxide 86-90 early growth response 1 Rattus norvegicus 99-104 30939252-10 2019 These data show that H2O2 enhances the expression of Egr-1, which was associated with increased phosphorylation of Akt, and H2O2 triggers its effects on Egr-1 expression through c-Src-mediated Akt and CREB-dependent signaling events in VSMCs. Hydrogen Peroxide 21-25 early growth response 1 Rattus norvegicus 53-58 30939252-10 2019 These data show that H2O2 enhances the expression of Egr-1, which was associated with increased phosphorylation of Akt, and H2O2 triggers its effects on Egr-1 expression through c-Src-mediated Akt and CREB-dependent signaling events in VSMCs. Hydrogen Peroxide 21-25 early growth response 1 Rattus norvegicus 153-158 27176126-8 2016 In addition, KY-0520 and KY-0538 reduced the phosphorylation of ERK1/2 and p38-MAPK proteins, and inhibited the translocation of Egr-1 from the cytoplasm to nucleus in H2O2-treated H9c2 cells. Hydrogen Peroxide 168-172 early growth response 1 Rattus norvegicus 129-134 24365880-12 2014 In H9C2 rat cardiomyoblast cell line, EGR-1 siRNA downregulated hydrogen peroxide-induced EGR-1 mRNA expression and subsequently reduced tumor necrosis factor alpha mRNA level. Hydrogen Peroxide 64-81 early growth response 1 Rattus norvegicus 38-43 24365880-12 2014 In H9C2 rat cardiomyoblast cell line, EGR-1 siRNA downregulated hydrogen peroxide-induced EGR-1 mRNA expression and subsequently reduced tumor necrosis factor alpha mRNA level. Hydrogen Peroxide 64-81 early growth response 1 Rattus norvegicus 90-95 10190566-4 1999 H2O2 preferentially induced the expression of c-fos, c-jun, c-myc and egr-1, while JunB and JunD levels remained almost unchanged. Hydrogen Peroxide 0-4 early growth response 1 Rattus norvegicus 70-75