PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 10187851-0 1999 FAK is the upstream signal protein of the phosphatidylinositol 3-kinase-Akt survival pathway in hydrogen peroxide-induced apoptosis of a human glioblastoma cell line. Hydrogen Peroxide 96-113 AKT serine/threonine kinase 1 Homo sapiens 72-75 10428852-4 1999 Exogenous H(2)O(2) (50-200 microM) also stimulates Akt/PKB phosphorylation (maximal 8- +/- 0.2-fold increase), suggesting that Akt/PKB activation is redox-sensitive. Hydrogen Peroxide 10-18 AKT serine/threonine kinase 1 Homo sapiens 51-54 10428852-4 1999 Exogenous H(2)O(2) (50-200 microM) also stimulates Akt/PKB phosphorylation (maximal 8- +/- 0.2-fold increase), suggesting that Akt/PKB activation is redox-sensitive. Hydrogen Peroxide 10-18 AKT serine/threonine kinase 1 Homo sapiens 55-58 10428852-4 1999 Exogenous H(2)O(2) (50-200 microM) also stimulates Akt/PKB phosphorylation (maximal 8- +/- 0.2-fold increase), suggesting that Akt/PKB activation is redox-sensitive. Hydrogen Peroxide 10-18 AKT serine/threonine kinase 1 Homo sapiens 127-130 10428852-4 1999 Exogenous H(2)O(2) (50-200 microM) also stimulates Akt/PKB phosphorylation (maximal 8- +/- 0.2-fold increase), suggesting that Akt/PKB activation is redox-sensitive. Hydrogen Peroxide 10-18 AKT serine/threonine kinase 1 Homo sapiens 131-134 10428852-5 1999 Both angiotensin II and H(2)O(2) stimulation of Akt/PKB are abrogated by the phosphatidylinositol 3-kinase (PI3-K) inhibitors wortmannin and LY294002 (2(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one), suggesting that PI3-K is an upstream mediator of Akt/PKB activation in VSMCs. Hydrogen Peroxide 24-32 AKT serine/threonine kinase 1 Homo sapiens 48-51 10428852-5 1999 Both angiotensin II and H(2)O(2) stimulation of Akt/PKB are abrogated by the phosphatidylinositol 3-kinase (PI3-K) inhibitors wortmannin and LY294002 (2(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one), suggesting that PI3-K is an upstream mediator of Akt/PKB activation in VSMCs. Hydrogen Peroxide 24-32 AKT serine/threonine kinase 1 Homo sapiens 52-55 10428852-5 1999 Both angiotensin II and H(2)O(2) stimulation of Akt/PKB are abrogated by the phosphatidylinositol 3-kinase (PI3-K) inhibitors wortmannin and LY294002 (2(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one), suggesting that PI3-K is an upstream mediator of Akt/PKB activation in VSMCs. Hydrogen Peroxide 24-32 AKT serine/threonine kinase 1 Homo sapiens 250-253 10428852-5 1999 Both angiotensin II and H(2)O(2) stimulation of Akt/PKB are abrogated by the phosphatidylinositol 3-kinase (PI3-K) inhibitors wortmannin and LY294002 (2(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one), suggesting that PI3-K is an upstream mediator of Akt/PKB activation in VSMCs. Hydrogen Peroxide 24-32 AKT serine/threonine kinase 1 Homo sapiens 254-257 10428852-6 1999 Furthermore, diphenylene iodonium, an inhibitor of flavin-containing oxidases, or overexpression of catalase to block angiotensin II-induced intracellular H(2)O(2) production significantly inhibits angiotensin II-induced Akt/PKB phosphorylation, indicating a role for ROS in agonist-induced Akt/PKB activation. Hydrogen Peroxide 155-163 AKT serine/threonine kinase 1 Homo sapiens 221-224 10428852-6 1999 Furthermore, diphenylene iodonium, an inhibitor of flavin-containing oxidases, or overexpression of catalase to block angiotensin II-induced intracellular H(2)O(2) production significantly inhibits angiotensin II-induced Akt/PKB phosphorylation, indicating a role for ROS in agonist-induced Akt/PKB activation. Hydrogen Peroxide 155-163 AKT serine/threonine kinase 1 Homo sapiens 225-228 10428852-6 1999 Furthermore, diphenylene iodonium, an inhibitor of flavin-containing oxidases, or overexpression of catalase to block angiotensin II-induced intracellular H(2)O(2) production significantly inhibits angiotensin II-induced Akt/PKB phosphorylation, indicating a role for ROS in agonist-induced Akt/PKB activation. Hydrogen Peroxide 155-163 AKT serine/threonine kinase 1 Homo sapiens 291-294 10428852-6 1999 Furthermore, diphenylene iodonium, an inhibitor of flavin-containing oxidases, or overexpression of catalase to block angiotensin II-induced intracellular H(2)O(2) production significantly inhibits angiotensin II-induced Akt/PKB phosphorylation, indicating a role for ROS in agonist-induced Akt/PKB activation. Hydrogen Peroxide 155-163 AKT serine/threonine kinase 1 Homo sapiens 295-298 10187851-2 1999 Hydrogen peroxide (1 mM) markedly induced tyrosine phosphorylation of focal adhesion kinase (FAK) and serine phosphorylation of Akt at 1 h after stimulation. Hydrogen Peroxide 0-17 AKT serine/threonine kinase 1 Homo sapiens 128-131 10187851-7 1999 Thus, it became apparent that FAK is the upstream signal protein of the PI 3-kinase-Akt survival pathway in hydrogen peroxide-induced apoptosis in T98G cells. Hydrogen Peroxide 108-125 AKT serine/threonine kinase 1 Homo sapiens 84-87 33775716-0 2021 The signaling pathway PI3K/Akt/Nrf2/HO-1 plays a role in the mitochondrial protection promoted by astaxanthin in the SH-SY5Y cells exposed to hydrogen peroxide. Hydrogen Peroxide 142-159 AKT serine/threonine kinase 1 Homo sapiens 27-30 33775716-9 2021 Inhibition of the PI3K/Akt or of the HO-1 enzyme abolished the AST-induced mitochondrial protection in cells challenged with H2O2. Hydrogen Peroxide 125-129 AKT serine/threonine kinase 1 Homo sapiens 23-26 33775716-11 2021 Thus, we suggest that AST promotes mitochondrial protection by a mechanism dependent on the PI3K/Akt/Nrf2/HO-1 signaling pathway in SH-SY5Y cells exposed to H2O2. Hydrogen Peroxide 157-161 AKT serine/threonine kinase 1 Homo sapiens 97-100 34854518-2 2022 In the present study, we found that SC66, a pyridine-based allosteric Akt inhibitor, suppressed basal and H2 O2 -induced autophagy concurrent with decreased phosphorylation and activity of AMPK. Hydrogen Peroxide 106-111 AKT serine/threonine kinase 1 Homo sapiens 70-73 33793833-0 2021 Fluvastatin protects neuronal cells from hydrogen peroxide-induced toxicity with decreasing oxidative damage and increasing PI3K/Akt/mTOR signalling. Hydrogen Peroxide 41-58 AKT serine/threonine kinase 1 Homo sapiens 129-132 34394836-9 2021 In conclusion, our findings suggested that LEO can ameliorate the oxidative stress damage and insufficient angiogenesis of HUVECs induced by H2O2 through activating the PI3K/Akt-eNOS signaling pathway. Hydrogen Peroxide 141-145 AKT serine/threonine kinase 1 Homo sapiens 174-177 34873406-9 2021 In addition, Que increased the activation of phosphorylation of PI3K and AKT decreased by H2O2. Hydrogen Peroxide 90-94 AKT serine/threonine kinase 1 Homo sapiens 73-76 34769014-5 2021 We further identify that ApoE2 and ApoE3 regulate Akt/FoxO3a/Bim signaling pathway in the presence of H2O2. Hydrogen Peroxide 102-106 AKT serine/threonine kinase 1 Homo sapiens 50-53 34558653-11 2021 In addition, RK1 treatment notably induced Nrf2 nuclear translocation, increased the protein expression levels of Nrf2 and HO-1, and the ratio of phosphorylated-AKT to AKT in the PIG1 cells exposed to H2O2. Hydrogen Peroxide 201-205 AKT serine/threonine kinase 1 Homo sapiens 161-164 34558653-11 2021 In addition, RK1 treatment notably induced Nrf2 nuclear translocation, increased the protein expression levels of Nrf2 and HO-1, and the ratio of phosphorylated-AKT to AKT in the PIG1 cells exposed to H2O2. Hydrogen Peroxide 201-205 AKT serine/threonine kinase 1 Homo sapiens 168-171 34091997-5 2021 Mechanistically, AQP3-mediated H2 O2 uptake increased intracellular ROS levels to inactivate PTEN and activate the AKT/mTOR pathway subsequently inhibit autophagy and 1promote proliferation in LUAD cells. Hydrogen Peroxide 31-36 AKT serine/threonine kinase 1 Homo sapiens 115-118 34077701-0 2021 Formononetin attenuates H2O2-induced cell death through decreasing ROS level by PI3K/Akt-Nrf2-activated antioxidant gene expression and suppressing MAPK-regulated apoptosis in neuronal SH-SY5Y cells. Hydrogen Peroxide 24-28 AKT serine/threonine kinase 1 Homo sapiens 85-88 35400456-0 2022 Combined RNA-seq and molecular biology technology revealed the protective effect of Cyclocarya paliurus polysaccharide on H2O2-induced oxidative damage in L02 cells thought regulating mitochondrial function, oxidative stress and PI3K/Akt and MAPK signaling pathways. Hydrogen Peroxide 122-126 AKT serine/threonine kinase 1 Homo sapiens 234-237 34316226-13 2021 H2O2 exposure stimulated activation of caspase 3 and significantly reduced phosphorylated AKT at all concentrations used. Hydrogen Peroxide 0-4 AKT serine/threonine kinase 1 Homo sapiens 90-93 35499687-3 2022 Moreover, PI3K/AKT pathway participates in the process of myocardial injury induced by a number of substances such as H2O2, Mercury, lipopolysaccharides, adriamycin, doxorubicin and epirubicin. Hydrogen Peroxide 118-122 AKT serine/threonine kinase 1 Homo sapiens 15-18 35543385-9 2022 Further, Klotho pretreatment enhanced Akt phosphorylation and expression as well as nuclear translocation of Nrf2 in H2O2-treated ARPE-19 cells. Hydrogen Peroxide 117-121 AKT serine/threonine kinase 1 Homo sapiens 38-41 35198094-11 2022 Meanwhile, we observed that the expression of TGF-beta2, p-AKT, and p-GSK-3beta was upregulated in H2O2-treated ARPE-19 cells and downregulated in luteolin-treated cells, revealing that luteolin inhibited the activation of the AKT/GSK-3beta pathway. Hydrogen Peroxide 99-103 AKT serine/threonine kinase 1 Homo sapiens 59-62 35085541-7 2022 Further results indicated that inhibition of IRE1 suppressed H2O2 induced cell senescence, IL-1beta-induced cellular reactive oxygen species (ROS) level and the activation of NF-kappaB, PI3K/Akt and MAPK signaling pathways. Hydrogen Peroxide 61-65 AKT serine/threonine kinase 1 Homo sapiens 191-194 35222803-10 2022 As demonstrated by these data, KGF-2 resisted H2O2-mediated apoptosis and oxidative stress in HLECs through Nrf2/HO-1 and PI3K/Akt pathways, suggesting a potential protective effect against the formation of cataracts. Hydrogen Peroxide 46-50 AKT serine/threonine kinase 1 Homo sapiens 127-130 35387349-0 2022 Metformin Promotes Differentiation and Attenuates H2O2-Induced Oxidative Damage of Osteoblasts via the PI3K/AKT/Nrf2/HO-1 Pathway. Hydrogen Peroxide 50-54 AKT serine/threonine kinase 1 Homo sapiens 108-111 35387349-11 2022 In conclusion, our study revealed that metformin promoted osteogenic differentiation and H2O2-induced oxidative damage of osteoblasts via the PI3K/AKT/Nrf2/HO-1 pathway. Hydrogen Peroxide 89-93 AKT serine/threonine kinase 1 Homo sapiens 147-150 35198094-11 2022 Meanwhile, we observed that the expression of TGF-beta2, p-AKT, and p-GSK-3beta was upregulated in H2O2-treated ARPE-19 cells and downregulated in luteolin-treated cells, revealing that luteolin inhibited the activation of the AKT/GSK-3beta pathway. Hydrogen Peroxide 99-103 AKT serine/threonine kinase 1 Homo sapiens 227-230 35204748-6 2022 Moreover, phosphorylation of the apoptotic p53 protein was reduced while the activity of the AKT/mTOR signaling pathway was increased, which was further enhanced by oxidative stress (H2O2) in granulocytes and erythroleukemic K562 cells. Hydrogen Peroxide 183-187 AKT serine/threonine kinase 1 Homo sapiens 93-96 33521822-9 2021 In addition, cell experiments showed that EC exhibited antioxidant effects in an H2O2-mediated OS model in ovarian granulosa cells by regulating the expression of PI3K/AKT/Nrf2 signaling pathway and multiple downstream antioxidant enzymes. Hydrogen Peroxide 81-85 AKT serine/threonine kinase 1 Homo sapiens 168-171 34949154-11 2022 DHCR24 was down-regulated in H2O2-induced ALI, and overexpression of DHCR24 could inhibit H2O2-induced oxidative stress and apoptosis of A549 cells by activating the Phosphatidylinositol-3-Kinase/Protein Kinase B (PI3K/AKT) signaling pathway. Hydrogen Peroxide 90-94 AKT serine/threonine kinase 1 Homo sapiens 219-222 32794021-9 2021 Cytotoxicity of hydrogen peroxide significantly increased in HUVEC cells pre-treated with Hcy for 24 h. CONCLUSIONS: These data indicate that Hcy induces an increase in cellular ferritin level, and the process is mediated by alterations in Akt-FOXO3a signaling pathway. Hydrogen Peroxide 16-33 AKT serine/threonine kinase 1 Homo sapiens 240-243 33915898-6 2021 Western blotting detection indicated that H2O2 elicited hyperphosphorylation of PI3K/Akt, ERK1/2, JNK, p38 MAPK, and p65 NF-kappaB. Hydrogen Peroxide 42-46 AKT serine/threonine kinase 1 Homo sapiens 85-88 33226571-6 2021 Our results also showed that melatonin increased the activity of PI3K/AKT and DNA-PKcs knockdown in melatonin-treated HUVECs that lead to inactivation of PI3K/AKT signaling under oxidative stress with H2O2. Hydrogen Peroxide 201-205 AKT serine/threonine kinase 1 Homo sapiens 70-73 33545736-10 2021 N-Acetyl cysteine (NAC), a ROS scavenger, markedly abolished H2O2-stimulated PI3K/Akt/GSK3beta signaling, caspase-3 activation, and phosphatidylserine exposure. Hydrogen Peroxide 61-65 AKT serine/threonine kinase 1 Homo sapiens 82-85 33226571-6 2021 Our results also showed that melatonin increased the activity of PI3K/AKT and DNA-PKcs knockdown in melatonin-treated HUVECs that lead to inactivation of PI3K/AKT signaling under oxidative stress with H2O2. Hydrogen Peroxide 201-205 AKT serine/threonine kinase 1 Homo sapiens 159-162 33226571-7 2021 Furthermore, blockade of PI3K/AKT signal with LY294002 significantly reduced melatonin-induced apoptosis inhibition in H2O2-treated HUVECs. Hydrogen Peroxide 119-123 AKT serine/threonine kinase 1 Homo sapiens 30-33 33226571-8 2021 Taken together, our findings identify a miR-101/DNA-PKcs/PI3K/AKT signaling pathway in melatonin-induced endothelial cell apoptosis inhibition under oxidative stress with H2O2. Hydrogen Peroxide 171-175 AKT serine/threonine kinase 1 Homo sapiens 62-65 33111210-0 2021 6,4"-dihydroxy-7-methoxyflavanone protects against H2O2-induced cellular senescence by inducing SIRT1 and inhibiting phosphatidylinositol 3-kinase/Akt pathway activation. Hydrogen Peroxide 51-55 AKT serine/threonine kinase 1 Homo sapiens 147-150 33111210-9 2021 Additionally, Akt and ERK were activated with different kinetics after H2O2 exposure, and Akt activity inhibition attenuated SA-beta-gal activity augmentation. Hydrogen Peroxide 71-75 AKT serine/threonine kinase 1 Homo sapiens 14-17 33111210-10 2021 We also found that DMF inhibited H2O2-induced Akt phosphorylation. Hydrogen Peroxide 33-37 AKT serine/threonine kinase 1 Homo sapiens 46-49 33385639-0 2021 Tectorigenin protect HUVECs from H2O2-induced oxidative stress injury by regulating PI3K/Akt pathway. Hydrogen Peroxide 33-37 AKT serine/threonine kinase 1 Homo sapiens 89-92 33385639-13 2021 Furthermore, Tectorigenin increased the ratios of p-PI3K to PI3K and p-Akt to Akt in H2O2-induced HUVECs. Hydrogen Peroxide 85-89 AKT serine/threonine kinase 1 Homo sapiens 71-74 33385639-13 2021 Furthermore, Tectorigenin increased the ratios of p-PI3K to PI3K and p-Akt to Akt in H2O2-induced HUVECs. Hydrogen Peroxide 85-89 AKT serine/threonine kinase 1 Homo sapiens 78-81 33385639-15 2021 Tectorigenin protected HUVECs against H2O2-induced OSI via PI3K/Akt pathway. Hydrogen Peroxide 38-42 AKT serine/threonine kinase 1 Homo sapiens 64-67 33393627-8 2021 Furthermore, hCypA activates the PI3K/Akt/mTOR pathway in A549 cells in response to H2O2 stimulation. Hydrogen Peroxide 84-88 AKT serine/threonine kinase 1 Homo sapiens 38-41 33393627-10 2021 This study showed that CypA protected A549 cells from H2O2-induced oxidative injury and apoptosis by activating the PI3K/Akt/mTOR pathway. Hydrogen Peroxide 54-58 AKT serine/threonine kinase 1 Homo sapiens 121-124 32678390-3 2020 The exposure of adipocytes to H2O2 (generated by glucose oxidase) resulted in an elevated level of ROS, a reduced content of GSH and an increase in JNK activation, concomitantly with a decrease in insulin-stimulated PI3K, Akt and p70S6K activation and cellular glucose uptake. Hydrogen Peroxide 30-34 AKT serine/threonine kinase 1 Homo sapiens 222-225 32860809-13 2020 These results implicated that puerarin exhibited cytoprotective effects against H2O2-induced EndMT in HCAECs through alleviating oxidative stress, activating the PI3K/AKT pathway and limiting cell migration. Hydrogen Peroxide 80-84 AKT serine/threonine kinase 1 Homo sapiens 167-170 33002038-0 2020 Thermal cycling protects SH-SY5Y cells against hydrogen peroxide and beta-amyloid-induced cell injury through stress response mechanisms involving Akt pathway. Hydrogen Peroxide 47-64 AKT serine/threonine kinase 1 Homo sapiens 147-150 32556702-8 2020 Meanwhile, PI3K/Akt signaling pathway is involved in the anti-H2O2 and BACE1 inhibition effect of YS-5-23. Hydrogen Peroxide 62-66 AKT serine/threonine kinase 1 Homo sapiens 16-19 32598944-7 2020 Further validation of the involved key signaling molecules, using an in vitro model of hydrogen peroxide-induced neurotoxicity, revealed that both the inhibition of PKA signaling pathway or the silencing of Akt reversed the neuroprotective action of artemisinin on motoneurons. Hydrogen Peroxide 87-104 AKT serine/threonine kinase 1 Homo sapiens 207-210 32598944-8 2020 Our results indicate that artemisinin provides neuroprotection against axotomy and hydrogen peroxide-induced neurotoxicity, an effect that might be mediated by the PKA-Akt signaling pathway. Hydrogen Peroxide 83-100 AKT serine/threonine kinase 1 Homo sapiens 168-171 32590330-10 2020 Addition of extracellular H2O2 had only a nominal effect on phosphorylation of eNOS, kinase Akt or AMP-activated protein kinase (AMPK). Hydrogen Peroxide 26-30 AKT serine/threonine kinase 1 Homo sapiens 92-95 33101583-6 2020 We also found that hydrogen peroxide or BSO induces the phosphorylation of Akt and IKK of endothelial differentiated sphere cells. Hydrogen Peroxide 19-36 AKT serine/threonine kinase 1 Homo sapiens 75-78 33101583-7 2020 Accordingly, both Akt1/2 kinase inhibitor and BAY 11-7082 inhibited hydrogen peroxide and BSO-mediated endothelial differentiation of cancer stem-like sphere cells. Hydrogen Peroxide 68-85 AKT serine/threonine kinase 1 Homo sapiens 18-24 32881386-3 2020 Here, I show that SHIP2 inhibition in cervical cancer cell lines alters H2 O2 -mediated AKT and MAPK/ERK pathway activation. Hydrogen Peroxide 72-77 AKT serine/threonine kinase 1 Homo sapiens 88-91 32471716-6 2020 It was confirmed that MDM2 could regulate lens epithelial cell apoptosis, and MDM2 inhibitors could partly inhibited AKT"s role in suppressing apoptosis induced by H2O2. Hydrogen Peroxide 164-168 AKT serine/threonine kinase 1 Homo sapiens 117-120 32774685-8 2020 Pretreatment with the inhibitor of PI3K/Akt signaling (LY294002) completely blocked these EE-TT-upregulated mRNA expressions and abolished the improvement of cell viability in H2O2-treated ARPE-19 cells. Hydrogen Peroxide 176-180 AKT serine/threonine kinase 1 Homo sapiens 40-43 32387419-7 2020 Esc-1GN significantly reversed the dysregulation of MAPK, AKT and NF-kappaB signaling caused by H2O2. Hydrogen Peroxide 96-100 AKT serine/threonine kinase 1 Homo sapiens 58-61 32271398-9 2020 RESULTS: Our research showed that collagen II, aggrecan, PI3K, and Akt markedly decreased in IL-1beta- or H2O2-induced degenerated NP cells. Hydrogen Peroxide 106-110 AKT serine/threonine kinase 1 Homo sapiens 67-70 32043704-0 2020 Repression of Kisspeptin1 weakens hydrogen peroxide-caused injury in HTR8 cells via adjusting PI3K/AKT/mTOR pathway. Hydrogen Peroxide 34-51 AKT serine/threonine kinase 1 Homo sapiens 99-102 32043704-11 2020 Eventually, we observed that the repression of KISS1 triggered the activation of PI3K/AKT/mTOR in HTR8 cells under H2 O2 management. Hydrogen Peroxide 115-120 AKT serine/threonine kinase 1 Homo sapiens 86-89 30979953-7 2019 GB treatment resulted in activation of Lyn, Akt, and ERK1/2, suggesting that GB is able to mitigate the H2O2-induced oxidative stress via activation of both the Lyn/PI3K/Akt and ERK/MAPK pathways. Hydrogen Peroxide 104-108 AKT serine/threonine kinase 1 Homo sapiens 44-47 31940823-3 2020 We aimed to evaluate the PI3K/AKT/FoxO3a pathway as a mechanism of cell survival in lung epithelial A549 cells exposed to PM10 that were subsequently challenged with hydrogen peroxide (H2O2). Hydrogen Peroxide 166-183 AKT serine/threonine kinase 1 Homo sapiens 30-33 30738142-9 2019 Interestingly, cells lacking BVR-A and treated with H2O2 showed reduced GSK-3beta inhibition and increased Tau Ser404 phosphorylation, which resulted from a defect of Akt and GSK-3beta physical interaction. Hydrogen Peroxide 52-56 AKT serine/threonine kinase 1 Homo sapiens 167-170 31698268-10 2020 However, the Akt activator (SC79) and mTOR activator (MHY1485) suppressed the autophagy level induced by monotropein in H2O2-treated cells. Hydrogen Peroxide 120-124 AKT serine/threonine kinase 1 Homo sapiens 13-16 31062616-10 2019 Finally, Sal activated PI3K/AKT and Wnt/beta-catenin pathways through enhancement of miR-27a in H2O2-injured HTM cells. Hydrogen Peroxide 96-100 AKT serine/threonine kinase 1 Homo sapiens 28-31 31062616-11 2019 In conclusion, these discoveries suggested that Sal could protect HTM cells against H2O2-evoked oxidative damage by activating PI3K/AKT and Wnt/beta-catenin pathways through enhancement of miR-27a. Hydrogen Peroxide 84-88 AKT serine/threonine kinase 1 Homo sapiens 132-135 31283994-7 2019 (4) DAla2GIP inhibited the H2O2 mediated inflammation by up- regulating the expressions of Sox9 and Col2a1 and inhibiting PI3K/Akt/NF-kappaB pathway. Hydrogen Peroxide 27-31 AKT serine/threonine kinase 1 Homo sapiens 127-130 31283994-9 2019 Further, molecular biology experiments confirmed that DAla2GIP inhibited the H2O2 mediated inflammation vis up-regulating the expressions of Sox9 and Col2a1 and inhibiting PI3K/Akt/NF-kappaB pathway. Hydrogen Peroxide 77-81 AKT serine/threonine kinase 1 Homo sapiens 177-180 31173752-8 2019 These finding provided evidences that liraglutide protected the H2O2 induced AD-like neurodegeneration through improving Akt/GSK-3beta signaling pathway. Hydrogen Peroxide 64-68 AKT serine/threonine kinase 1 Homo sapiens 121-124 31049492-0 2019 Wheat alkylresorcinols protect human retinal pigment epithelial cells against H2O2-induced oxidative damage through Akt-dependent Nrf2/HO-1 signaling. Hydrogen Peroxide 78-82 AKT serine/threonine kinase 1 Homo sapiens 116-119 30576222-0 2019 Klotho protein inhibits H2O2-induced oxidative injury in endothelial cells via regulation of PI3K/AKT/Nrf2/HO-1 pathways. Hydrogen Peroxide 24-28 AKT serine/threonine kinase 1 Homo sapiens 98-101 30576222-8 2019 Klotho protein enhanced the antioxidant defense ability of the cells by activating the PI3K/AKT pathway, which upregulated the expression of Nrf2/HO-1, thereby inhibiting H2O2-induced oxidative damage. Hydrogen Peroxide 171-175 AKT serine/threonine kinase 1 Homo sapiens 92-95 30979953-7 2019 GB treatment resulted in activation of Lyn, Akt, and ERK1/2, suggesting that GB is able to mitigate the H2O2-induced oxidative stress via activation of both the Lyn/PI3K/Akt and ERK/MAPK pathways. Hydrogen Peroxide 104-108 AKT serine/threonine kinase 1 Homo sapiens 170-173 30959964-0 2019 Annexin A2 Regulates AKT Upon H2O2-Dependent Signaling Activation in Cancer Cells. Hydrogen Peroxide 30-34 AKT serine/threonine kinase 1 Homo sapiens 21-24 31011561-8 2019 By regards to RT-PCR and western blotting data, although expression of Akt-1, Bcl-2 and Bax was not change considerably but phosphorylated Akt-1 (pAkt-1) was up regulated after treatment with 20 muM H2O2 compared to control group. Hydrogen Peroxide 199-203 AKT serine/threonine kinase 1 Homo sapiens 139-144 30456590-6 2019 In addition, a robust activation of PI3K/Akt/mTOR and AMPK pathways was also observed in H2O2 treated cells. Hydrogen Peroxide 89-93 AKT serine/threonine kinase 1 Homo sapiens 41-44 31217063-0 2019 NANOG Alleviates the Damage of Human Hair Follicle Mesenchymal Stem Cells Caused by H2O2 through Activation of AKT Pathway. Hydrogen Peroxide 84-88 AKT serine/threonine kinase 1 Homo sapiens 111-114 31217063-5 2019 When treated with 400 mumol/L H2O2, NANOG hHF-MSCs showed higher cell survival rate, lower ROS production and apoptosis, higher expression of p-AKT, higher ratio of p-AKT/AKT. Hydrogen Peroxide 30-34 AKT serine/threonine kinase 1 Homo sapiens 144-147 31217063-5 2019 When treated with 400 mumol/L H2O2, NANOG hHF-MSCs showed higher cell survival rate, lower ROS production and apoptosis, higher expression of p-AKT, higher ratio of p-AKT/AKT. Hydrogen Peroxide 30-34 AKT serine/threonine kinase 1 Homo sapiens 167-170 31217063-5 2019 When treated with 400 mumol/L H2O2, NANOG hHF-MSCs showed higher cell survival rate, lower ROS production and apoptosis, higher expression of p-AKT, higher ratio of p-AKT/AKT. Hydrogen Peroxide 30-34 AKT serine/threonine kinase 1 Homo sapiens 167-170 31217063-6 2019 CONCLUSION: Our results suggest that NANOG could protect hHF-MSCs against cell damage caused by H2O2 through activating AKT signaling pathway. Hydrogen Peroxide 96-100 AKT serine/threonine kinase 1 Homo sapiens 120-123 30690126-5 2019 Also, vernicilignan A increase the cell viability of H2O2 treated cells via the activation of Akt and GSK3beta. Hydrogen Peroxide 53-57 AKT serine/threonine kinase 1 Homo sapiens 94-97 30709701-0 2019 Oxyresveratrol protects human lens epithelial cells against hydrogen peroxide-induced oxidative stress and apoptosis by activation of Akt/HO-1 pathway. Hydrogen Peroxide 60-77 AKT serine/threonine kinase 1 Homo sapiens 134-137 30709701-9 2019 Oxy increased p-Akt and HO-1 expressions in H2O2-stimulated HLECs. Hydrogen Peroxide 44-48 AKT serine/threonine kinase 1 Homo sapiens 16-19 30709701-10 2019 Akt and HO-1 expressions form a regulatory axis and Oxy activated the Akt/HO-1 pathway in H2O2-stimulated HLECs. Hydrogen Peroxide 90-94 AKT serine/threonine kinase 1 Homo sapiens 0-3 30709701-10 2019 Akt and HO-1 expressions form a regulatory axis and Oxy activated the Akt/HO-1 pathway in H2O2-stimulated HLECs. Hydrogen Peroxide 90-94 AKT serine/threonine kinase 1 Homo sapiens 70-73 30709701-11 2019 Inhibition of the Akt/HO-1 pathway by LY294002 or ZnPP attenuated the effects of Oxy on oxidative stress and apoptosis in H2O2-stimulated HLECs. Hydrogen Peroxide 122-126 AKT serine/threonine kinase 1 Homo sapiens 18-21 30709701-12 2019 In conclusion, Oxy protected H2O2-induced oxidative stress and apoptosis through activating the Akt/HO-1 pathway, suggesting the protective effect of Oxy against H2O2-induced cataract. Hydrogen Peroxide 29-33 AKT serine/threonine kinase 1 Homo sapiens 96-99 30709701-12 2019 In conclusion, Oxy protected H2O2-induced oxidative stress and apoptosis through activating the Akt/HO-1 pathway, suggesting the protective effect of Oxy against H2O2-induced cataract. Hydrogen Peroxide 162-166 AKT serine/threonine kinase 1 Homo sapiens 96-99 30485137-7 2019 NAC reversed H2O2-activated MAPK and Akt/mammalian target of rapamycin pathways in dose-dependent manners. Hydrogen Peroxide 13-17 AKT serine/threonine kinase 1 Homo sapiens 37-40 30594603-13 2019 LBPs activated PI3K/AKT and ERK signaling pathways through up-regulation of miR-4295 in H2O2-injured HTM cells. Hydrogen Peroxide 88-92 AKT serine/threonine kinase 1 Homo sapiens 20-23 30689624-0 2019 Sulforaphane Attenuates H2O2-induced Oxidant Stress in Human Trabecular Meshwork Cells (HTMCs) via the Phosphatidylinositol 3-Kinase (PI3K)/Serine/Threonine Kinase (Akt)-Mediated Factor-E2-Related Factor 2 (Nrf2) Signaling Activation. Hydrogen Peroxide 24-28 AKT serine/threonine kinase 1 Homo sapiens 165-168 30689624-8 2019 CONCLUSIONS These results indicated a novel application for SFN in attenuating H2O2-induced oxidative stress in HTMCs through activating PI3K/Akt/Nrf2 signaling pathway. Hydrogen Peroxide 79-83 AKT serine/threonine kinase 1 Homo sapiens 142-145 30669455-5 2019 We also show that in GAB-transfected cells treated with H2O2, the PI3K/AKT pathway is less induced compared to the pcDNA-transfected counterparts and that pretreatment with PDGF-BB, an activator of AKT, protects GAB-transfected cells from death caused by the H2O2 treatment. Hydrogen Peroxide 56-60 AKT serine/threonine kinase 1 Homo sapiens 71-74 30669455-5 2019 We also show that in GAB-transfected cells treated with H2O2, the PI3K/AKT pathway is less induced compared to the pcDNA-transfected counterparts and that pretreatment with PDGF-BB, an activator of AKT, protects GAB-transfected cells from death caused by the H2O2 treatment. Hydrogen Peroxide 56-60 AKT serine/threonine kinase 1 Homo sapiens 198-201 30669455-5 2019 We also show that in GAB-transfected cells treated with H2O2, the PI3K/AKT pathway is less induced compared to the pcDNA-transfected counterparts and that pretreatment with PDGF-BB, an activator of AKT, protects GAB-transfected cells from death caused by the H2O2 treatment. Hydrogen Peroxide 259-263 AKT serine/threonine kinase 1 Homo sapiens 71-74 30697266-3 2019 Stimulation with H2O2 leads to increased oxidative stress and activation of the AKT/mTOR pathway. Hydrogen Peroxide 17-21 AKT serine/threonine kinase 1 Homo sapiens 80-83 30697266-4 2019 However, the role of renin-angiotensin system inhibitors in oxidative stress-induced endothelial cell dysfunction and H2O2-induced AKT activation remains unclear. Hydrogen Peroxide 118-122 AKT serine/threonine kinase 1 Homo sapiens 131-134 30697266-12 2019 Co-treatment with captopril and the AKT inhibitor MK-2206 reduced the H2O2-induced P53 and ICAM-1 protein expression (p < 0.05). Hydrogen Peroxide 70-74 AKT serine/threonine kinase 1 Homo sapiens 36-39 30697266-14 2019 Conclusions: The study revealed the protective effect of captopril against H2O2-induced endothelial cell dysfunction through the AKT/mTOR pathway, and its enhancement of cell survival. Hydrogen Peroxide 75-79 AKT serine/threonine kinase 1 Homo sapiens 129-132 30585261-13 2018 These data suggest that the NO suppresses H2O2-induced SK-N-MC cell apoptosis by suppressing apoptosis signal mediating the interaction between 14-3-3beta and Bad phosphorylation via PKG/PI3K/Akt. Hydrogen Peroxide 42-46 AKT serine/threonine kinase 1 Homo sapiens 192-195 30485823-7 2018 Conversely, increased mitochondrial-H2O2 formation disrupts Akt1-mediated phosphorylation of Hsp70, and non-phosphorylatable Hsp70 mutants decrease SOD2 import, resulting in mitochondrial oxidative stress. Hydrogen Peroxide 36-40 AKT serine/threonine kinase 1 Homo sapiens 60-64 30316911-0 2018 Protective Effects of L-3-n-Butylphthalide Against H2O2-Induced Injury in Neural Stem Cells by Activation of PI3K/Akt and Mash1 Pathway. Hydrogen Peroxide 51-55 AKT serine/threonine kinase 1 Homo sapiens 114-117 30515391-0 2018 S-Allyl Cysteine Alleviates Hydrogen Peroxide Induced Oxidative Injury and Apoptosis through Upregulation of Akt/Nrf-2/HO-1 Signaling Pathway in HepG2 Cells. Hydrogen Peroxide 28-45 AKT serine/threonine kinase 1 Homo sapiens 109-112 30282966-8 2018 Transduction of primary RGC with any of the membrane-associated VEGF constructs increased survival both under normal culture conditions and in the presence of the cytotoxic chemicals H2O2 (via VEGFR2/PI3K/AKT signaling) and N-methyl-D-aspartate (via reduced Ca2+ influx). Hydrogen Peroxide 183-187 AKT serine/threonine kinase 1 Homo sapiens 205-208 29956734-0 2018 MicroRNA-26a protects vascular smooth muscle cells against H2O2-induced injury through activation of the PTEN/AKT/mTOR pathway. Hydrogen Peroxide 59-63 AKT serine/threonine kinase 1 Homo sapiens 110-113 30119249-8 2018 Moreover, we found that H2O2 decreased Akt and eNOS phosphorylation, which perhaps, indirectly reduced nitric oxide (NO) production. Hydrogen Peroxide 24-28 AKT serine/threonine kinase 1 Homo sapiens 39-42 30119249-12 2018 Furthermore, BEG reduced H2O2-induced endothelial cells injury by the involvement of antioxidation and PI3K/Akt/eNOS/NO signaling pathways. Hydrogen Peroxide 25-29 AKT serine/threonine kinase 1 Homo sapiens 108-111 29902453-3 2018 In OB-6 human osteoblasts, Gab1 silencing (by targeted-shRNA) or complete knockout (by CRISPR-Cas9 KO plasmid) largely attenuated Akt activation by H2O2. Hydrogen Peroxide 148-152 AKT serine/threonine kinase 1 Homo sapiens 130-133 30210588-9 2018 In addition, pretreatment with SAL significantly increased the phosphorylation levels of Akt and GSK-3beta in H2O2-treated ARPE-19 cells. Hydrogen Peroxide 110-114 AKT serine/threonine kinase 1 Homo sapiens 89-92 30210588-10 2018 In conclusion, the present study demonstrated that SAL protected RPE cells against H2O2-induced cell injury through the activation of the Akt/GSK-3beta signaling pathway. Hydrogen Peroxide 83-87 AKT serine/threonine kinase 1 Homo sapiens 138-141 29956734-15 2018 These results indicated that miR-26a protected VSMCs against H2O2-induced injury through activation of the PTEN/AKT/mTOR pathway, and miR-26a may be considered as a potential prognostic biomarker and therapeutic target in the treatment of AAA. Hydrogen Peroxide 61-65 AKT serine/threonine kinase 1 Homo sapiens 112-115 29115459-0 2018 Isolariciresinol-9"-O-alpha-L-arabinofuranoside protects against hydrogen peroxide-induced apoptosis of human umbilical vein endothelial cells via a PI3K/Akt/Bad-dependent pathway. Hydrogen Peroxide 65-82 AKT serine/threonine kinase 1 Homo sapiens 154-157 29847774-0 2018 Wall shear stress promotes intimal hyperplasia through the paracrine H2O2-mediated NOX-AKT-SVV axis. Hydrogen Peroxide 69-73 AKT serine/threonine kinase 1 Homo sapiens 87-90 29847774-5 2018 SIGNIFICANCE: We demonstrated that the elevation of WSS promotes VSMC proliferation and migration through the H2O2-mediated NOX-AKT-SVV axis, thereby accelerating IH development. Hydrogen Peroxide 110-114 AKT serine/threonine kinase 1 Homo sapiens 128-131 29512905-0 2018 Geniposide prevents H2 O2 -induced oxidative damage in melanocytes by activating the PI3K-Akt signalling pathway. Hydrogen Peroxide 20-25 AKT serine/threonine kinase 1 Homo sapiens 90-93 29512905-9 2018 Furthermore, geniposide increased the levels of p-Akt and regulated the expression of downstream proteins in the PI3K-Akt pathway, such as Bcl-2, Bax, and cleaved caspase 3 and 9, in H2 O2 -treated melanocytes. Hydrogen Peroxide 183-188 AKT serine/threonine kinase 1 Homo sapiens 118-121 29922854-9 2018 In addition, NAC reversed fMLP- and H2O2-induced activation of Akt and RhoA-GTPase. Hydrogen Peroxide 36-40 AKT serine/threonine kinase 1 Homo sapiens 63-66 29512729-15 2018 To summarize, these results demonstrated that curcumin was able to prevent SOD-driven H2O2-induced pancreatic cancer metastasis by blocking the PI3K/Akt/NF-kappaB signaling pathway. Hydrogen Peroxide 86-90 AKT serine/threonine kinase 1 Homo sapiens 149-152 29512729-16 2018 The use of curcumin to inhibit the H2O2/Akt/NF-kappaB axis may be a promising therapeutic approach to the treatment of patients with pancreatic cancer. Hydrogen Peroxide 35-39 AKT serine/threonine kinase 1 Homo sapiens 40-43 29687528-6 2018 Furthermore, nobiletin significantly increased Akt phosphorylation in ARPE-19 cells exposed to H2 O2 . Hydrogen Peroxide 95-100 AKT serine/threonine kinase 1 Homo sapiens 47-50 29579543-7 2018 Moreover, the senescent and H2O2-induced old cells showed higher expression levels of p-PI3K, Akt-1, p-mTOR, p-FoxO1 and FoxO1 than young cells. Hydrogen Peroxide 28-32 AKT serine/threonine kinase 1 Homo sapiens 94-99 29059467-5 2018 Here, we showed using cell lines derived from human HCC that H2 O2 , which is a major component of ROS in living organisms, elongates telomeres by increasing telomerase activity through protein kinase B (AKT) activation. Hydrogen Peroxide 61-66 AKT serine/threonine kinase 1 Homo sapiens 204-207 29059467-11 2018 CONCLUSION: We showed that H2 O2 contributes to telomere elongation through AKT activation in advanced HCC, suggesting that an AKT inhibitor such as perifosine may be useful for treating patients with malignant HCC. Hydrogen Peroxide 27-32 AKT serine/threonine kinase 1 Homo sapiens 76-79 29059467-11 2018 CONCLUSION: We showed that H2 O2 contributes to telomere elongation through AKT activation in advanced HCC, suggesting that an AKT inhibitor such as perifosine may be useful for treating patients with malignant HCC. Hydrogen Peroxide 27-32 AKT serine/threonine kinase 1 Homo sapiens 127-130 29444190-8 2018 Therefore, exosomal miR-21 derived from H2O2-treated MSCs could be transported to C-kit+ cardiac stem cells to functionally inhibit PTEN expression, thereby activating PI3K/AKT signaling and leading to protection against oxidative stress-triggered cell death. Hydrogen Peroxide 40-44 AKT serine/threonine kinase 1 Homo sapiens 173-176 29324335-4 2018 In particular, four compounds were found to protect SH-SY5Y cells from H2O2-induced toxicity by increasing Bcl-2/Bax ratio, regulating PI3-K/Akt cascade and inhibiting the ERK pathway. Hydrogen Peroxide 71-75 AKT serine/threonine kinase 1 Homo sapiens 141-144 29462594-10 2018 Therefore, these outcomes propose that melatonin protects iPSC-derived NSCs from H2O2-induced injury through the mediation of melatonin receptor and PI3K/AKT signaling pathway. Hydrogen Peroxide 81-85 AKT serine/threonine kinase 1 Homo sapiens 154-157 28933316-8 2018 The photo-activated Akt conferred protection against nutritional deprivation and H2O2 stresses to the cells significantly. Hydrogen Peroxide 81-85 AKT serine/threonine kinase 1 Homo sapiens 20-23 29207024-12 2018 In conclusion, the present study revealed that geraniin protects MSCs from H2O2-induced oxidative stress injury via the PI3K/Akt pathway. Hydrogen Peroxide 75-79 AKT serine/threonine kinase 1 Homo sapiens 125-128 29115459-8 2018 Furthermore, the protein expression of phosphatidylinositol 3-kinase (PI3K), phosphorylated-Akt and Bcl-2-associated agonist of cell death (Bad) were increased, and reduced caspase-3 activation was observed, following treatment with MWS-19 in H2O2-treated HUVECs. Hydrogen Peroxide 243-247 AKT serine/threonine kinase 1 Homo sapiens 92-95 28978811-0 2017 Tetramethylpyrazine Protects Bone Marrow-Derived Mesenchymal Stem Cells against Hydrogen Peroxide-Induced Apoptosis through PI3K/Akt and ERK1/2 Pathways. Hydrogen Peroxide 80-97 AKT serine/threonine kinase 1 Homo sapiens 129-132 28978811-9 2017 In conclusion, our results confirm that TMP protects BMSCs against H2O2-induced apoptosis by regulating the PI3K/Akt and ERK1/2 signaling pathways, suggesting that TMP may be used in combination with BMSCs to improve cell survival for the treatment of ischemic stroke. Hydrogen Peroxide 67-71 AKT serine/threonine kinase 1 Homo sapiens 113-116 29115489-6 2018 Furthermore, isorhamnetin pretreatment significantly increased the phosphorylation of phosphoinositide 3-kinase (PI3K) and AKT serine/threonine kinase 1 (Akt) in RPE cells exposed to H2O2, compared with cells treated with H2O2 alone. Hydrogen Peroxide 183-187 AKT serine/threonine kinase 1 Homo sapiens 123-152 29115489-6 2018 Furthermore, isorhamnetin pretreatment significantly increased the phosphorylation of phosphoinositide 3-kinase (PI3K) and AKT serine/threonine kinase 1 (Akt) in RPE cells exposed to H2O2, compared with cells treated with H2O2 alone. Hydrogen Peroxide 183-187 AKT serine/threonine kinase 1 Homo sapiens 154-157 29115489-6 2018 Furthermore, isorhamnetin pretreatment significantly increased the phosphorylation of phosphoinositide 3-kinase (PI3K) and AKT serine/threonine kinase 1 (Akt) in RPE cells exposed to H2O2, compared with cells treated with H2O2 alone. Hydrogen Peroxide 222-226 AKT serine/threonine kinase 1 Homo sapiens 154-157 29560097-5 2018 SC79 activated Akt in the neuronal cells, which was required for its neuroprotection against H2O2. Hydrogen Peroxide 93-97 AKT serine/threonine kinase 1 Homo sapiens 15-18 29560097-7 2018 Further, CRISPR-Cas9-mediated Akt1 knockout in SH-SY5Y cells abolished SC79-induced neuroprotective function against H2O2. Hydrogen Peroxide 117-121 AKT serine/threonine kinase 1 Homo sapiens 30-34 29560097-8 2018 Reversely, forced activation of Akt by the constitutively-active Akt1 mimicked SC79-induced anti-H2O2 activity. Hydrogen Peroxide 97-101 AKT serine/threonine kinase 1 Homo sapiens 32-35 29560097-8 2018 Reversely, forced activation of Akt by the constitutively-active Akt1 mimicked SC79-induced anti-H2O2 activity. Hydrogen Peroxide 97-101 AKT serine/threonine kinase 1 Homo sapiens 65-69 29560097-9 2018 Together, we conclude that activation of Akt by SC79 protects dopaminergic neurons from H2O2. Hydrogen Peroxide 88-92 AKT serine/threonine kinase 1 Homo sapiens 41-44 29115459-10 2018 In conclusion, the results of the present study indicate that MWS-19 may protect against H2O2-induced HUVEC apoptosis via the PI3K/Akt/Bad signaling pathway. Hydrogen Peroxide 89-93 AKT serine/threonine kinase 1 Homo sapiens 131-134 28211035-0 2017 Resveratrol decreases FoXO protein expression through PI3K-Akt-dependent pathway inhibition in H2O2-treated synoviocytes. Hydrogen Peroxide 95-99 AKT serine/threonine kinase 1 Homo sapiens 59-62 28211035-8 2017 Taken together, these findings indicate that Res can induce apoptosis in H2O2-treated FLSs in part by inhibiting the PI3K-Akt signaling pathway. Hydrogen Peroxide 73-77 AKT serine/threonine kinase 1 Homo sapiens 122-125 29039467-7 2017 Treatment of the H2O2-injured cortical neurons with conditioned media from hMSCs (hMSC-CM) increased the phosphorylation of Akt, reduced cell death and mitochondrial translocation of Bax, and regulated extracellular levels of IGF-1 and IGF-2. Hydrogen Peroxide 17-21 AKT serine/threonine kinase 1 Homo sapiens 124-127 28870012-8 2017 In addition, lactoferrin reduced hydrogen peroxide-induced apoptosis via inhibition of caspase-3 and Akt activation. Hydrogen Peroxide 33-50 AKT serine/threonine kinase 1 Homo sapiens 101-104 26657242-5 2017 In addition, H2O2 treatment increased LAT1 and CD98 expression, as well as an activated form of AKT and mTOR in MMNK1 and CCA cell lines (KKU-M055 and KKU-M213). Hydrogen Peroxide 13-17 AKT serine/threonine kinase 1 Homo sapiens 96-99 28738491-12 2017 (4) The effect of H(2)O(2) on signaling molecules in PAECs: there was a significant increase in phosphorylation level of Akt in PAECs incubated with H(2)O(2) for 30 minutes compared to that in control PAECs (P<0.01), while there was no significant difference in levels of Akt between H(2)O(2) treated PAECs and control PAECs. Hydrogen Peroxide 18-26 AKT serine/threonine kinase 1 Homo sapiens 121-124 28738491-12 2017 (4) The effect of H(2)O(2) on signaling molecules in PAECs: there was a significant increase in phosphorylation level of Akt in PAECs incubated with H(2)O(2) for 30 minutes compared to that in control PAECs (P<0.01), while there was no significant difference in levels of Akt between H(2)O(2) treated PAECs and control PAECs. Hydrogen Peroxide 18-26 AKT serine/threonine kinase 1 Homo sapiens 275-278 28738491-14 2017 Conclusion: H(2)O(2) at the concentration of 400 mumol/L could induce human PAECs injuries via the regulation of Akt and MAPK signaling pathways. Hydrogen Peroxide 12-20 AKT serine/threonine kinase 1 Homo sapiens 113-116 28716056-10 2017 Furthermore, H2O2 treatment partly improved cell viability and up regulated p-PI3K and p-AKT in HASMCs. Hydrogen Peroxide 13-17 AKT serine/threonine kinase 1 Homo sapiens 89-92 27510267-0 2017 Neuroserpin Attenuates H2O2-Induced Oxidative Stress in Hippocampal Neurons via AKT and BCL-2 Signaling Pathways. Hydrogen Peroxide 23-27 AKT serine/threonine kinase 1 Homo sapiens 80-83 28469544-0 2017 Fucoxanthin prevents H2O2-induced neuronal apoptosis via concurrently activating the PI3-K/Akt cascade and inhibiting the ERK pathway. Hydrogen Peroxide 21-25 AKT serine/threonine kinase 1 Homo sapiens 91-94 28469544-5 2017 H2O2 treatment led to the reduced activity of phosphoinositide 3-kinase (PI3-K)/Akt cascade and the increased activity of extracellular signal-regulated kinase (ERK) pathway in SH-SY5Y cells. Hydrogen Peroxide 0-4 AKT serine/threonine kinase 1 Homo sapiens 80-83 28469544-6 2017 Moreover, fucoxanthin significantly restored the altered activities of PI3-K/Akt and ERK pathways induced by H2O2. Hydrogen Peroxide 109-113 AKT serine/threonine kinase 1 Homo sapiens 77-80 28469544-9 2017 Conclusions: Our data strongly revealed that fucoxanthin protected against H2O2-induced neurotoxicity via concurrently activating the PI3-K/Akt cascade and inhibiting the ERK pathway, providing support for the use of fucoxanthin to treat neurodegenerative disorders induced by oxidative stress. Hydrogen Peroxide 75-79 AKT serine/threonine kinase 1 Homo sapiens 140-143 28831286-6 2017 H2O2-induced hyperactivation of the phosphatidylinositol 3-kinase/protein kinase B (AKT) signaling pathway was suppressed by KPE through regulated expression of forkhead box O3a (FoxO3a) and mammalian target of rapamycin (mTOR). Hydrogen Peroxide 0-4 AKT serine/threonine kinase 1 Homo sapiens 84-87 28503420-10 2017 H2O2 inhibited ARPE-19 cell viability and proliferation and also suppressed EGF-stimulated increase of RPE cell viability and proliferation by affecting the EGFR/AKT signaling pathway. Hydrogen Peroxide 0-4 AKT serine/threonine kinase 1 Homo sapiens 162-165 28503420-13 2017 In addition, antioxidant NAC attenuated H2O2-induced inhibition of ARPE-19 cell viability through alleviating reduction of EGFR, and phosphorylated and total AKT proteins. Hydrogen Peroxide 40-44 AKT serine/threonine kinase 1 Homo sapiens 158-161 28383489-0 2017 Sulfated Hetero-Polysaccharides Protect SH-SY5Y Cells from H2O2-Induced Apoptosis by Affecting the PI3K/Akt Signaling Pathway. Hydrogen Peroxide 59-63 AKT serine/threonine kinase 1 Homo sapiens 104-107 28126675-0 2017 Rutin attenuates H2O2-induced oxidation damage and apoptosis in Leydig cells by activating PI3K/Akt signal pathways. Hydrogen Peroxide 17-21 AKT serine/threonine kinase 1 Homo sapiens 96-99 28126675-8 2017 Furthermore, 20mumol/L rutin significantly abrogated the decrease in levels of phosphoinositide 3-kinase (PI3K) and phosphorylated serine/threonine kinase (p-AKT) induced by H2O2. Hydrogen Peroxide 174-178 AKT serine/threonine kinase 1 Homo sapiens 158-161 28126675-10 2017 Taken together, these results suggest that rutin attenuates H2O2-induced oxidation damage and apoptosis in Leydig cells by activating PI3K/Akt signal pathways, providing a promising strategy to decrease oxidative stress associated with male infertility. Hydrogen Peroxide 60-64 AKT serine/threonine kinase 1 Homo sapiens 139-142 27930977-6 2017 Furthermore, eupatilin pretreatment markedly enhanced phosphorylation levels of PI3K and Akt in ARPE-19 cells exposed to H2O2. Hydrogen Peroxide 121-125 AKT serine/threonine kinase 1 Homo sapiens 89-92 27930977-7 2017 In conclusion, our data showed that eupatilin protected against H2O2-induced oxidative stress and apoptosis through the activation of PI3K/Akt signaling pathway in ARPE-19 cells. Hydrogen Peroxide 64-68 AKT serine/threonine kinase 1 Homo sapiens 139-142 28367274-6 2017 Moreover, apoptotic conditions induced inactivation of Akt in a time-dependent manner similar to TXNIP expression and H2O2 treatment led to Akt inactivation. Hydrogen Peroxide 118-122 AKT serine/threonine kinase 1 Homo sapiens 140-143 27924507-0 2016 TPPU protects tau from H2O2-induced hyperphosphorylation in HEK293/tau cells by regulating PI3K/AKT/GSK-3beta pathway. Hydrogen Peroxide 23-27 AKT serine/threonine kinase 1 Homo sapiens 96-99 28694916-0 2017 The Protective Effect of Indole-3-Acetic Acid (IAA) on H2O2-Damaged Human Dental Pulp Stem Cells Is Mediated by the AKT Pathway and Involves Increased Expression of the Transcription Factor Nuclear Factor-Erythroid 2-Related Factor 2 (Nrf2) and Its Downstream Target Heme Oxygenase 1 (HO-1). Hydrogen Peroxide 55-59 AKT serine/threonine kinase 1 Homo sapiens 116-119 28694916-9 2017 Moreover, IAA treatment protected hDPSCs against H2O2-induced oxidative stress via increased expression of Nrf2 and HO-1, mediated by the AKT pathway. Hydrogen Peroxide 49-53 AKT serine/threonine kinase 1 Homo sapiens 138-141 29270200-5 2017 PRGF treatment induces phosphorylation of AKT preventing ASC death induced by lethal concentrations of hydrogen peroxide. Hydrogen Peroxide 103-120 AKT serine/threonine kinase 1 Homo sapiens 42-45 29270200-6 2017 Indeed, AKT inhibition abolished the PRGF apoptosis prevention in ASC exposed to 100 muM of hydrogen peroxide. Hydrogen Peroxide 92-109 AKT serine/threonine kinase 1 Homo sapiens 8-11 27924507-12 2016 In conclusion, these results suggested that the protective effect of TPPU on H2O2-induced oxidative stress is associated with PI3K/Akt/GSK-3beta pathway. Hydrogen Peroxide 77-81 AKT serine/threonine kinase 1 Homo sapiens 131-134 27800716-11 2016 CONCLUSION: These findings suggest that protective effects of MQA against H2O2-induced apoptosis might be associated with mitochondrial apoptosis, ERK1/2 and AKT/GSK-3beta pathway. Hydrogen Peroxide 74-78 AKT serine/threonine kinase 1 Homo sapiens 158-161 27494022-0 2016 PI-103 and Quercetin Attenuate PI3K-AKT Signaling Pathway in T- Cell Lymphoma Exposed to Hydrogen Peroxide. Hydrogen Peroxide 89-106 AKT serine/threonine kinase 1 Homo sapiens 36-39 27494022-7 2016 Present study is aimed to analyze PI3K-AKT signaling pathway in H2O2 exposed Dalton"s lymphoma ascite (DLA) cells. Hydrogen Peroxide 64-68 AKT serine/threonine kinase 1 Homo sapiens 39-42 27494022-9 2016 Exposure of H2O2 (1mM H2O2 for 30min) to DLA cells caused ROS accumulation and resulted in increased phosphorylation of PI3K and downstream proteins PDK1 and AKT (Ser-473 and Thr-308), cell survival factors BAD and ERK1/2, as well as TNFR1. Hydrogen Peroxide 12-16 AKT serine/threonine kinase 1 Homo sapiens 158-161 27494022-9 2016 Exposure of H2O2 (1mM H2O2 for 30min) to DLA cells caused ROS accumulation and resulted in increased phosphorylation of PI3K and downstream proteins PDK1 and AKT (Ser-473 and Thr-308), cell survival factors BAD and ERK1/2, as well as TNFR1. Hydrogen Peroxide 22-26 AKT serine/threonine kinase 1 Homo sapiens 158-161 26889770-6 2016 Further experiments revealed that l-carnitine pretreatment enhanced the phosphorylation of Akt in H2O2-treated cells. Hydrogen Peroxide 98-102 AKT serine/threonine kinase 1 Homo sapiens 91-94 27144349-5 2016 The PI3K/AKT/c-MYC signalling axis is important in this context, since pharmacologic blockade of PI3K-AKT or siRNA knockdown of c-MYC effectively inhibited the RR phenotype and its associated CSC-like features, as well as the H2 O2 -induced RU/RR conversion. Hydrogen Peroxide 226-231 AKT serine/threonine kinase 1 Homo sapiens 9-12 27530947-15 2016 CONCLUSION: The FGVL can reduce hydrogen peroxide induced oxidative injury in HUVEC by increasing the level of nitric oxide through PI3K/Akt/eNOS pathway. Hydrogen Peroxide 32-49 AKT serine/threonine kinase 1 Homo sapiens 137-140 27154977-10 2016 Altogether, these results underscore a critical role for H2O2-Cer-Akt signaling axis in the antileukemic action of SNG. Hydrogen Peroxide 57-61 AKT serine/threonine kinase 1 Homo sapiens 66-69 26095111-10 2016 It was found that WEHDW reversed H2O2-induced activation of MEK/ERK pathway and H2O2-induced inhibition of P13-K/AKT/GSK3beta pathway in LO2 cells. Hydrogen Peroxide 80-84 AKT serine/threonine kinase 1 Homo sapiens 113-116 26889770-9 2016 Altogether, these results demonstrate that l-carnitine protects HL7702 cells against H2O2-induced cell damage through Akt-mediated activation of Nrf2 signaling pathway. Hydrogen Peroxide 85-89 AKT serine/threonine kinase 1 Homo sapiens 118-121 26983852-4 2016 Mechanistic study revealed that SIRT6 induced autophagy via attenuation of AKT signaling and treatment with autophagy inhibitor 3-MA or knockdown of autophagy-related protein Atg5 rescued H2O2-induced neuronal injury. Hydrogen Peroxide 188-192 AKT serine/threonine kinase 1 Homo sapiens 75-78 26525360-0 2016 Diazoxide protects L6 skeletal myoblasts from H2O2-induced apoptosis via the phosphatidylinositol-3 kinase/Akt pathway. Hydrogen Peroxide 46-50 AKT serine/threonine kinase 1 Homo sapiens 107-110 27007368-0 2016 Isoquercitrin Inhibits Hydrogen Peroxide-Induced Apoptosis of EA.hy926 Cells via the PI3K/Akt/GSK3beta Signaling Pathway. Hydrogen Peroxide 23-40 AKT serine/threonine kinase 1 Homo sapiens 90-93 27007368-9 2016 These results demonstrated that the anti-apoptotic effect of isoquercitrin on H2O2-induced EA.hy926 cells was likely associated with the regulation of isoquercitrin on Akt/GSK3beta signaling pathway and that isoquercitrin could be used clinically to interfere with the progression of endothelial injury-associated cardiovascular disease. Hydrogen Peroxide 78-82 AKT serine/threonine kinase 1 Homo sapiens 168-171 26769665-9 2016 Mechanistically, VEGF-C activated Akt signaling pathway via VEGF receptor 2, leading to a blockade of Bax expression and mitochondrial membrane translocation and thus protected cardiomyocyte from H2O2-induced activation of intrinsic apoptotic pathway. Hydrogen Peroxide 196-200 AKT serine/threonine kinase 1 Homo sapiens 34-37 26830227-6 2016 Intracellular H2O2 then oxidized PTEN and protein tyrosine phosphatase 1B (PTP1B) followed by activation of the Akt pathway. Hydrogen Peroxide 14-18 AKT serine/threonine kinase 1 Homo sapiens 112-115 26631726-8 2016 Stimulation of AKT by IGF-I, mimicked also by a constitutively active AKT mutant, reduced oxidative stress levels and cell death in H2O2-exposed astrocytes, boosting their neuroprotective action in co-cultured neurons. Hydrogen Peroxide 132-136 AKT serine/threonine kinase 1 Homo sapiens 15-18 26631726-8 2016 Stimulation of AKT by IGF-I, mimicked also by a constitutively active AKT mutant, reduced oxidative stress levels and cell death in H2O2-exposed astrocytes, boosting their neuroprotective action in co-cultured neurons. Hydrogen Peroxide 132-136 AKT serine/threonine kinase 1 Homo sapiens 70-73 26643608-0 2016 Dehydroepiandrosterone ameliorates H2O2-induced Leydig cells oxidation damage and apoptosis through inhibition of ROS production and activation of PI3K/Akt pathways. Hydrogen Peroxide 35-39 AKT serine/threonine kinase 1 Homo sapiens 152-155 26643608-8 2016 DHEA also reversed the decrease in PI3K and p-Akt protein levels induced by H2O2. Hydrogen Peroxide 76-80 AKT serine/threonine kinase 1 Homo sapiens 46-49 26643608-9 2016 These data showed that DHEA could ameliorate H2O2-induced oxidative damage by increasing anti-oxidative enzyme activities, which resulted in reduced ROS content, and decreased apoptosis, mainly by preventing the loss of DeltaPsim and inhibiting caspase-3 protein levels via activation of PI3K/Akt signaling pathways. Hydrogen Peroxide 45-49 AKT serine/threonine kinase 1 Homo sapiens 293-296 26456053-6 2015 Mechanistically, H2O2-induced cell death involves inhibition of pro-survival signaling proteins (Akt, Nrf2) and activation of pro-death signaling proteins (p38, JNK1). Hydrogen Peroxide 17-21 AKT serine/threonine kinase 1 Homo sapiens 97-100 27803763-0 2016 miR-21 Reduces Hydrogen Peroxide-Induced Apoptosis in c-kit+ Cardiac Stem Cells In Vitro through PTEN/PI3K/Akt Signaling. Hydrogen Peroxide 15-32 AKT serine/threonine kinase 1 Homo sapiens 107-110 27803763-7 2016 Taken together, these results indicate that the anti-H2O2-induced apoptosis effect of miR-21 in c-kit+ CSC is contributed by PTEN/PI3K/Akt signaling. Hydrogen Peroxide 53-57 AKT serine/threonine kinase 1 Homo sapiens 135-138 26505797-9 2015 Escin-induced RPE cytoprotection against H2O2 was also alleviated by the AKT inhibitors. Hydrogen Peroxide 41-45 AKT serine/threonine kinase 1 Homo sapiens 73-76 26456053-7 2015 Overexpression of TRPV2 in H2O2-treated hepatoma cells aggravates the inhibition of Akt and Nrf2, while it enhances the activation of p38 and JNK1 at the early stage of cell death. Hydrogen Peroxide 27-31 AKT serine/threonine kinase 1 Homo sapiens 84-87 26495060-7 2015 Furthermore, propofol significantly ameliorated H2O2-induced phosphorylation of p38 MAPK, JNK, and Akt in HUVECs. Hydrogen Peroxide 48-52 AKT serine/threonine kinase 1 Homo sapiens 99-102 26495060-8 2015 CONCLUSIONS: Propofol can protect HUVECs against H2O2-induced apoptosis via a mechanism that may involve p38 MAPK, JNK, and Akt signaling pathways. Hydrogen Peroxide 49-53 AKT serine/threonine kinase 1 Homo sapiens 124-127 26360782-6 2015 Furthermore, BK treatment of H2O2-exposed cells leads to elevated phosphorylation of RB, AKT, and cyclin D1 compared with H2O2-treatment alone. Hydrogen Peroxide 29-33 AKT serine/threonine kinase 1 Homo sapiens 89-92 25720812-4 2015 In addition, pretreatment with THF attenuated H2O2-induced rapid and significant phosphorylation of c-Jun N-terminal kinase (JNK), p38 mitogen-activated protein kinase (MAPK), and phosphatidylinositol 3-kinases (PI3K)/Akt. Hydrogen Peroxide 46-50 AKT serine/threonine kinase 1 Homo sapiens 218-221 25963768-5 2015 Herein, we report for the first time that the hydrogen peroxide (H2O2), a redox signaling molecule, generated by these EHNs activates the endothelial nitric oxide synthase (eNOS) that promotes the nitric oxide (NO) production in a PI3K (phosphoinositide 3-kinase)/Akt dependent manner, eventually triggering angiogenesis. Hydrogen Peroxide 46-63 AKT serine/threonine kinase 1 Homo sapiens 264-267 25963768-5 2015 Herein, we report for the first time that the hydrogen peroxide (H2O2), a redox signaling molecule, generated by these EHNs activates the endothelial nitric oxide synthase (eNOS) that promotes the nitric oxide (NO) production in a PI3K (phosphoinositide 3-kinase)/Akt dependent manner, eventually triggering angiogenesis. Hydrogen Peroxide 65-69 AKT serine/threonine kinase 1 Homo sapiens 264-267 25720812-5 2015 THF also inhibited nuclear factor-kappaB (NF-kappaB) translocation to the nucleus induced by H2O2, down-stream of H2O2-induced phosphorylation of MAPKs and PI3K/Akt. Hydrogen Peroxide 114-118 AKT serine/threonine kinase 1 Homo sapiens 161-164 25644078-8 2015 We also found that H2O2 inhibited the PI3K/AKT and MAPK/ERK signalling pathways, whereas PTER treatment restored these signalling pathways. Hydrogen Peroxide 19-23 AKT serine/threonine kinase 1 Homo sapiens 43-46 25788646-15 2015 Furthermore, Grx1 overexpression prevented H2O2-induced AKT glutathionylation, resulting in a sustained phospho-AKT elevation in RPE cells. Hydrogen Peroxide 43-47 AKT serine/threonine kinase 1 Homo sapiens 56-59 25788646-15 2015 Furthermore, Grx1 overexpression prevented H2O2-induced AKT glutathionylation, resulting in a sustained phospho-AKT elevation in RPE cells. Hydrogen Peroxide 43-47 AKT serine/threonine kinase 1 Homo sapiens 112-115 25630653-8 2015 Atorvastatin activated H2O2-inhibited phosphorylation of Akt in a concentration-dependent manner. Hydrogen Peroxide 23-27 AKT serine/threonine kinase 1 Homo sapiens 57-60 25732085-7 2015 H2O2 treatment for short periods also stimulated phosphorylation of AKT and AMPK. Hydrogen Peroxide 0-4 AKT serine/threonine kinase 1 Homo sapiens 68-71 25630653-9 2015 The Akt inhibitor, LY294002, inhibited the effect of atorvastatin on inducing Akt phosphorylation and on suppressing H2O2-mediated caspase up-regulation and cell apoptosis. Hydrogen Peroxide 117-121 AKT serine/threonine kinase 1 Homo sapiens 4-7 25630653-10 2015 These findings indicate that atorvastatin protects cardiomyocyte from oxidative stress via inhibition of LOX-1 expression and apoptosis, and that activation of H2O2-inhibited phosphorylation of Akt may play an important role in the protective function of atorvastatin. Hydrogen Peroxide 160-164 AKT serine/threonine kinase 1 Homo sapiens 194-197 25633408-10 2015 Taken together, our data suggest that LPA rescues H2O2-induced apoptosis mainly by interacting with Gi-coupled LPA3, resulting activation of the ERK1/2- and PI3 K/AKT-pathways and inhibition caspase-3 cleavage, and LPA protection of BMSCs against the apoptosis is independent of it induced autophagy. Hydrogen Peroxide 50-54 AKT serine/threonine kinase 1 Homo sapiens 163-166 25997250-7 2015 Propofol also inhibited H2O2-induced p38 MAPK, JNK and Akt phosphorylation. Hydrogen Peroxide 24-28 AKT serine/threonine kinase 1 Homo sapiens 55-58 26279427-15 2015 CONCLUSIONS: Collectively, these results suggest that PRDX6 protects ARPE-19 cells from H2O2-induced oxidative stress and apoptosis and that this protection is mediated at least partially through the PI3K/AKT pathway. Hydrogen Peroxide 88-92 AKT serine/threonine kinase 1 Homo sapiens 205-208 25452142-0 2014 Exendin-4 protects adipose-derived mesenchymal stem cells from apoptosis induced by hydrogen peroxide through the PI3K/Akt-Sfrp2 pathways. Hydrogen Peroxide 84-101 AKT serine/threonine kinase 1 Homo sapiens 119-122 25432585-0 2014 Wogonin modulates hydroperoxide-induced apoptosis via PI3K/Akt pathway in retinal pigment epithelium cells. Hydrogen Peroxide 18-31 AKT serine/threonine kinase 1 Homo sapiens 59-62 24875359-9 2014 MiR-124 decreases the H2O2-induced apoptosis of human hepatic L02 cells by up-regulating the activation of the AKT pathway. Hydrogen Peroxide 22-26 AKT serine/threonine kinase 1 Homo sapiens 111-114 25179747-7 2014 H2O2 (12.5 mumol/L) induced early stage apoptosis, significantly decreased Akt phosphorylation, and increased Bax protein level 4 h after stimulation. Hydrogen Peroxide 0-4 AKT serine/threonine kinase 1 Homo sapiens 75-78 25179747-10 2014 Exogenous H2O2 induces apoptosis and activation of autophagy in human adult retinal pigment epithelium cells through Akt-Bax signaling. Hydrogen Peroxide 10-14 AKT serine/threonine kinase 1 Homo sapiens 117-120 25069610-11 2014 H2O2-upregulated phosphorylation of JNK and p38 MAPK was dampened by adding astragalin to epithelial cells, while this compound enhanced epithelial activation of Akt and ERK. Hydrogen Peroxide 0-4 AKT serine/threonine kinase 1 Homo sapiens 162-165 24820448-9 2014 hUCBC paracrine factors increased in myocytes with H2O2 Akt activation by >= 25%, decreased JNK and p38 activation by > 35%, increased viability by > 22% and decreased apoptosis by > 33% (all P < 0.05). Hydrogen Peroxide 51-55 AKT serine/threonine kinase 1 Homo sapiens 56-59 24820448-10 2014 Akt inhibitor API-1 prevented the effects of hUCBC and enhanced H2O2 decrease of myocyte viability. Hydrogen Peroxide 64-68 AKT serine/threonine kinase 1 Homo sapiens 0-3 24875359-11 2014 Interference with the expression of the Rab38 gene can protect hepatic L02 from H2O2-induced apoptosis by increasing the phosphorylation of AKT. Hydrogen Peroxide 80-84 AKT serine/threonine kinase 1 Homo sapiens 140-143 24875359-14 2014 MiR-124 significantly decreases the H2O2-induced apoptosis of human hepatic L02 cells by targeting the Rab38 gene and activating the AKT pathway. Hydrogen Peroxide 36-40 AKT serine/threonine kinase 1 Homo sapiens 133-136 24802394-4 2014 Meanwhile, XIAP levels were decreased with 72 h of 250 muM H2O2 exposure, while there were also a decrease of JNK2, AKT, pAKT, and GSK3beta levels. Hydrogen Peroxide 59-63 AKT serine/threonine kinase 1 Homo sapiens 116-119 24802394-7 2014 These results suggest that, after 72 h of 250 muM H2O2 exposure, Akt, JNK, and GSK3beta intracellular kinase signaling pathways converge to regulate PDLC survival involving XIAP. Hydrogen Peroxide 50-54 AKT serine/threonine kinase 1 Homo sapiens 65-68 24534483-0 2014 Wogonin inhibits H2O2-induced angiogenesis via suppressing PI3K/Akt/NF-kappaB signaling pathway. Hydrogen Peroxide 17-21 AKT serine/threonine kinase 1 Homo sapiens 64-67 24456325-7 2014 The treatment of insulin had played a protective effect on H2O2-induced oxidative stress related to the Akt/Bcl-2 pathways. Hydrogen Peroxide 59-63 AKT serine/threonine kinase 1 Homo sapiens 104-107 24582688-5 2014 Second, H2O2, the most important form of ROS in the cells in response to extracellular stress signals, can induce phosphorylation of the EZH2 protein and the activation of JNK, STAT3, and Akt. Hydrogen Peroxide 8-12 AKT serine/threonine kinase 1 Homo sapiens 188-191 24582688-6 2014 By ectopic expression of the myc-tagged EZH2, we additionally identified direct interaction and phosphorylation of the EZH2 protein by Akt in response to arsenic and H2O2. Hydrogen Peroxide 166-170 AKT serine/threonine kinase 1 Homo sapiens 135-138 24534483-5 2014 Mechanistic studies showed that wogonin suppressed H2O2-activated PI3K/Akt pathway and reduced the expression of vascular endothelial growth factor (VEGF) up-regulated by H2O2 in both protein and mRNA levels. Hydrogen Peroxide 51-55 AKT serine/threonine kinase 1 Homo sapiens 71-74 24534483-7 2014 Then we further investigated the effect of wogonin on over-activated PI3K/Akt pathway by insulin-like growth factor-1 (IGF-1) and H2O2. Hydrogen Peroxide 130-134 AKT serine/threonine kinase 1 Homo sapiens 74-77 24534483-8 2014 We found that wogonin suppressed phosphorylation of Akt, up-regulation of VEGF and angiogenesis in vitro which was further induced by IGF-1 and H2O2. Hydrogen Peroxide 144-148 AKT serine/threonine kinase 1 Homo sapiens 52-55 24534483-10 2014 Taken together, these results suggested that wogonin was potential in inhibiting H2O2-induced angiogenesis in vitro and in vivo via suppressing PI3K/Akt pathway and NF-kappaB signaling. Hydrogen Peroxide 81-85 AKT serine/threonine kinase 1 Homo sapiens 149-152 25431609-5 2014 In addition, H2O2 produced transcriptional changes in p53, JNK, p38 MAPK, AKT, BAX, and CDK4 that were inclined towards apoptosis, while GBR-extracts showed some transcriptional changes (upregulation of BAX and p53) that suggested an inclination for apoptosis although other changes (upregulation of antioxidant genes, AKT, JNK, and p38 MAPK) suggested that GBR-extracts favored survival of the HepG2 cells. Hydrogen Peroxide 13-17 AKT serine/threonine kinase 1 Homo sapiens 74-77 25007762-6 2014 To determine the molecular mechanism responsible for FoxO6 modification by age-related oxidative stress, we examined H2O2-treated HEK293T cells in which FoxO6 was inactivated by phosphorylation and found that H2O2-induced oxidative stress promoted FoxO6 phosphorylation via PI3K/Akt signaling. Hydrogen Peroxide 117-121 AKT serine/threonine kinase 1 Homo sapiens 279-282 25007762-6 2014 To determine the molecular mechanism responsible for FoxO6 modification by age-related oxidative stress, we examined H2O2-treated HEK293T cells in which FoxO6 was inactivated by phosphorylation and found that H2O2-induced oxidative stress promoted FoxO6 phosphorylation via PI3K/Akt signaling. Hydrogen Peroxide 209-213 AKT serine/threonine kinase 1 Homo sapiens 279-282 24316214-8 2014 alpha-MSH-mediated S6K1 activation and pro-survival effect against H2O2 was inhibited by Akt inhibitors (perifosine, MK-2206 and LY294002). Hydrogen Peroxide 67-71 AKT serine/threonine kinase 1 Homo sapiens 89-92 25431609-5 2014 In addition, H2O2 produced transcriptional changes in p53, JNK, p38 MAPK, AKT, BAX, and CDK4 that were inclined towards apoptosis, while GBR-extracts showed some transcriptional changes (upregulation of BAX and p53) that suggested an inclination for apoptosis although other changes (upregulation of antioxidant genes, AKT, JNK, and p38 MAPK) suggested that GBR-extracts favored survival of the HepG2 cells. Hydrogen Peroxide 13-17 AKT serine/threonine kinase 1 Homo sapiens 319-322 24107596-0 2014 Resveratrol inhibits hydrogen peroxide-induced apoptosis in endothelial cells via the activation of PI3K/Akt by miR-126. Hydrogen Peroxide 21-38 AKT serine/threonine kinase 1 Homo sapiens 105-108 24107596-10 2014 The overexpression of miR-126 decreased PIK3R2(p85-beta) and enhanced Akt phosphorylation, which resulted in an increase in the survival of CRL-1730 cells exposed to H2O2. Hydrogen Peroxide 166-170 AKT serine/threonine kinase 1 Homo sapiens 70-73 24152913-3 2013 In addition, combinational treatment of fenobam with PEP-1-FK506BP significantly inhibited the activation of Akt and MAPK induced by H2O2, compared to treatment with PEP-1-FK506BP alone. Hydrogen Peroxide 133-137 AKT serine/threonine kinase 1 Homo sapiens 109-112 24868319-6 2014 The underlying mechanisms involve the inhibition of H2O2-induced activation of endothelial nitric oxide synthase (eNOS), adenosine monophosphate-activated protein kinase (AMPK), and Akt, as well as the redox sensitive transcription factor, NF-kappa B (NF- kappa B). Hydrogen Peroxide 52-56 AKT serine/threonine kinase 1 Homo sapiens 182-185 23845990-7 2013 Immunoblot analysis revealed that H2O2 increases the phosphorylation levels of AKT and MAPKs, exhibiting a cellular defense response; and consequently increases phospho-BAD levels. Hydrogen Peroxide 34-38 AKT serine/threonine kinase 1 Homo sapiens 79-82 24064382-7 2013 Further, Axl signaling activated by H2O2 treatment results in enhancement of cell migration via a PI3K/Akt-dependent pathway. Hydrogen Peroxide 36-40 AKT serine/threonine kinase 1 Homo sapiens 103-106 24064382-9 2013 Finally, downregulation of Akt1, but not Akt2, by RNAi in Axl-overexpressing cells inhibits the amount of activated Rac1 and the ability to migrate induced by H2O2 treatment. Hydrogen Peroxide 159-163 AKT serine/threonine kinase 1 Homo sapiens 27-31 24064382-10 2013 Together, these results show that a novel Axl-signaling cascade induced by H2O2 treatment triggers cell migration through the PI3K/Akt1/Rac1 pathway. Hydrogen Peroxide 75-79 AKT serine/threonine kinase 1 Homo sapiens 131-135 23418094-0 2013 Caffeoylserotonin protects human keratinocyte HaCaT cells against H2 O2 -induced oxidative stress and apoptosis through upregulation of HO-1 expression via activation of the PI3K/Akt/Nrf2 pathway. Hydrogen Peroxide 66-71 AKT serine/threonine kinase 1 Homo sapiens 179-182 23418094-7 2013 H2 O2 also induced the phosphorylation and subsequent activation of ERK, p38 MAPK, and Akt. Hydrogen Peroxide 0-5 AKT serine/threonine kinase 1 Homo sapiens 87-90 24042352-6 2013 Treatment of the cells with H2O2 also upregulated Akt mRNA and protein expression, and inhibited the binding of RUNX3 to the Akt promoter. Hydrogen Peroxide 28-32 AKT serine/threonine kinase 1 Homo sapiens 50-53 24042352-6 2013 Treatment of the cells with H2O2 also upregulated Akt mRNA and protein expression, and inhibited the binding of RUNX3 to the Akt promoter. Hydrogen Peroxide 28-32 AKT serine/threonine kinase 1 Homo sapiens 125-128 24042352-7 2013 The inverse correlation between the expression levels of RUNX3 and Akt in H2O2-treated cells was also associated with nuclear translocation of beta-catenin and upregulation of cyclin D1 expression, which induced cell proliferation. Hydrogen Peroxide 74-78 AKT serine/threonine kinase 1 Homo sapiens 67-70 23726950-11 2013 H2O2 treatment decreased expression of phospho-PI3K and phospho-Akt, which was reversed by MSP pretreatment. Hydrogen Peroxide 0-4 AKT serine/threonine kinase 1 Homo sapiens 64-67 23643711-9 2013 The elevated H2O2 production markedly decreased the PGC1alpha transcription, an effect that was accompanied by a reduced phosphorylation of Akt and CREB. Hydrogen Peroxide 13-17 AKT serine/threonine kinase 1 Homo sapiens 140-143 23954465-3 2013 We have previously reported that BBR attenuated H2O2 neurotoxicity via activating the PI3K/Akt/Nrf2-dependent pathway. Hydrogen Peroxide 48-52 AKT serine/threonine kinase 1 Homo sapiens 91-94 23726950-12 2013 These findings suggest that MSP attenuates H2O2-induced apoptosis in HK-2 cells by modulating the p38 and NF-kappaB, as well as PI3K/Akt, signaling pathways. Hydrogen Peroxide 43-47 AKT serine/threonine kinase 1 Homo sapiens 133-136 23685908-8 2013 The results showed that LM23 played a protective role in H2O2-induced apoptosis of 293T cells, mediated at least in part through the Akt/PI3K signal pathway. Hydrogen Peroxide 57-61 AKT serine/threonine kinase 1 Homo sapiens 133-136 23660824-7 2013 Reduced H2O2-induced apoptosis and activation of the cellular PI3K/Akt/mTOR pathway were demonstrated in HUVECs pretreated with salidroside. Hydrogen Peroxide 8-12 AKT serine/threonine kinase 1 Homo sapiens 67-70 23660824-11 2013 These findings suggested that salidroside was capable of protecting HUVECs against H2O2-induced apoptosis by activating the PI3K/Akt/mTOR-dependent pathway and inhibiting ROS production by activating REDD1. Hydrogen Peroxide 83-87 AKT serine/threonine kinase 1 Homo sapiens 129-132 23837937-9 2013 S1P markedly potentiated phosphorylation (activation) of Akt in the presence of H2 O2 . Hydrogen Peroxide 80-85 AKT serine/threonine kinase 1 Homo sapiens 57-60 23837937-10 2013 Wortmannin, a selective inhibitor of the PI3-K/Akt pathway, significantly suppressed S1P-induced attenuation of caspase-3 cleavage promoted by H2 O2 . Hydrogen Peroxide 143-148 AKT serine/threonine kinase 1 Homo sapiens 47-50 23837937-11 2013 CONCLUSIONS: S1P, a sphingolipid mediator, attenuates H2 O2 -induced apoptosis of HaCaT cells, by promoting phosphorylation of the Akt pathway. Hydrogen Peroxide 54-59 AKT serine/threonine kinase 1 Homo sapiens 131-134 23624332-7 2013 The use of pharmacological inhibitors as well as knockdown of ILK with small interfering RNA (siRNA) demonstrated the implication of a PI3K/ILK/AKT/ERK MAPK signaling pathway axis in the H2O2-induced TGF-beta1 overexpression. Hydrogen Peroxide 187-191 AKT serine/threonine kinase 1 Homo sapiens 144-147 23294370-10 2013 This cytotoxicity was at least partially attributed to the activation of the UPR pathway and consequent inactivation of AKT signaling, which resulted from the production of intracellular H2 O2 and causal release of endoplasmic reticulum Ca(2+) . Hydrogen Peroxide 187-192 AKT serine/threonine kinase 1 Homo sapiens 120-123 23261939-9 2013 The phosphorylation of Akt and ERK after exposure to H2O2 or MPP(+) was also inhibited in PINK1 P209A mutant cells compared with empty-vector-transfected cells. Hydrogen Peroxide 53-57 AKT serine/threonine kinase 1 Homo sapiens 23-26 23485815-8 2013 These data suggest that PF-mediated NPC protection on H2O2 injury is reliant on the activation of the PI3K/Akt-1 pathway, giving insight to an essential role of PF in NPC protection. Hydrogen Peroxide 54-58 AKT serine/threonine kinase 1 Homo sapiens 107-112 23685151-0 2013 Hydrogen peroxide inhibits transforming growth factor-beta1-induced cell cycle arrest by promoting Smad3 linker phosphorylation through activation of Akt-ERK1/2-linked signaling pathway. Hydrogen Peroxide 0-17 AKT serine/threonine kinase 1 Homo sapiens 150-153 23805040-9 2013 Both doses of H2O2 activated Akt, ERK1/2, and p38 in TM cells at 20 min after treatment, but not JNK or NFkB until 1 h after treatment. Hydrogen Peroxide 14-18 AKT serine/threonine kinase 1 Homo sapiens 29-32 23805040-12 2013 CONCLUSIONS: In TM cells, the PI3K-Akt, ERK, and p38 signaling pathways are primary oxidative stress response pathways involved in the mechanism of recovery from cellular morphologic changes induced by H2O2 treatment accompanied by actin cytoskeletal changes. Hydrogen Peroxide 202-206 AKT serine/threonine kinase 1 Homo sapiens 35-38 23750203-6 2013 Functional investigation demonstrated that AL-1 exerted its protective effects on H2O2-induced cell death of beta-cells by generating NADPH oxidase-dependent ROS to activate ERK1/2 and AKT1 signaling pathways. Hydrogen Peroxide 82-86 AKT serine/threonine kinase 1 Homo sapiens 185-189 23685151-5 2013 Mechanism studies showed that H2O2 increases the phosphorylation of Smad3 at the middle linker region in a concentration- and time-dependent manner and this effect is mediated by activation of extracellular signal-activated kinase 1/2 through Akt. Hydrogen Peroxide 30-34 AKT serine/threonine kinase 1 Homo sapiens 243-246 23528356-6 2013 In conclusion, the present study demonstrates that TMZ ameliorated H2O2-induced impairment of biological functions in EPCs with the involvement of antioxidation and Akt/eNOS signaling pathway. Hydrogen Peroxide 67-71 AKT serine/threonine kinase 1 Homo sapiens 165-168 23535373-4 2013 We verified the relationship between oxidative stress and BDNF signaling by treating primary hippocampal cultures with hydrogen peroxide (H2O2), which reduced BDNF and phosphorylated CREB and AKT (p-CREB, p-AKT) in a dose-dependent manner. Hydrogen Peroxide 119-136 AKT serine/threonine kinase 1 Homo sapiens 192-195 23535373-4 2013 We verified the relationship between oxidative stress and BDNF signaling by treating primary hippocampal cultures with hydrogen peroxide (H2O2), which reduced BDNF and phosphorylated CREB and AKT (p-CREB, p-AKT) in a dose-dependent manner. Hydrogen Peroxide 119-136 AKT serine/threonine kinase 1 Homo sapiens 207-210 23535373-4 2013 We verified the relationship between oxidative stress and BDNF signaling by treating primary hippocampal cultures with hydrogen peroxide (H2O2), which reduced BDNF and phosphorylated CREB and AKT (p-CREB, p-AKT) in a dose-dependent manner. Hydrogen Peroxide 138-142 AKT serine/threonine kinase 1 Homo sapiens 192-195 23535373-4 2013 We verified the relationship between oxidative stress and BDNF signaling by treating primary hippocampal cultures with hydrogen peroxide (H2O2), which reduced BDNF and phosphorylated CREB and AKT (p-CREB, p-AKT) in a dose-dependent manner. Hydrogen Peroxide 138-142 AKT serine/threonine kinase 1 Homo sapiens 207-210 24151624-6 2013 Likewise, the level of phosphorylated Akt, which activates Nrf2, was decreased by H2O2 treatment but restored by DHF treatment. Hydrogen Peroxide 82-86 AKT serine/threonine kinase 1 Homo sapiens 38-41 23533502-5 2013 In addition, ellagic acid also inhibited p38 MAPK and Akt phosphorylation stimulated by hydrogen peroxide. Hydrogen Peroxide 88-105 AKT serine/threonine kinase 1 Homo sapiens 54-57 22020565-14 2012 On the other hand, hydrogen peroxide induced Akt activation in PC-3 cells, which was abrogated in cells expressing p66ShcS36A. Hydrogen Peroxide 19-36 AKT serine/threonine kinase 1 Homo sapiens 45-48 23849871-2 2013 In the absence of insulin, H2O2 in the low micromolar range engages the canonical IRS-1/PI3K/Akt-dependent insulin signaling pathway, as well as other signaling elements (AMPK and p38 MAPK), to increase basal glucose transport activity. Hydrogen Peroxide 27-31 AKT serine/threonine kinase 1 Homo sapiens 93-96 23555559-12 2013 These results strongly suggest that the glucose deprivation-induced increase of H2O2 in the cells mediated the AKT phosphorylation. Hydrogen Peroxide 80-84 AKT serine/threonine kinase 1 Homo sapiens 111-114 23555559-13 2013 RNA interference of NOX4, but not of NOX5, completely suppressed the glucose deprivation-induced AKT phosphorylation as well as increase of the intracellular levels of ROS, whereas exogenous H2O2 could still induce AKT phosphorylation in the NOX4-knockdown cells. Hydrogen Peroxide 191-195 AKT serine/threonine kinase 1 Homo sapiens 215-218 22886427-7 2012 rhNRG-1 inhibited mPTP and activated Akt in the presence of H(2)O(2) and further protected the cells from H(2)O(2)-induced apoptosis. Hydrogen Peroxide 60-68 AKT serine/threonine kinase 1 Homo sapiens 37-40 22749807-7 2012 The H2O2 produced by HBC cells inhibited PP2A activity accompanied by increased phosphorylation of Akt and ERK1/2. Hydrogen Peroxide 4-8 AKT serine/threonine kinase 1 Homo sapiens 99-102 22749807-9 2012 We conclude that inhibition of catalase bioactivity by O2- leads to an increase in steady-state levels of H2O2 in HBC cells, which in turn inhibits PP2A activity, leading to phosphorylation of ERK 1/2 and Akt and resulting in HBC cell proliferation. Hydrogen Peroxide 106-110 AKT serine/threonine kinase 1 Homo sapiens 205-208 23460862-6 2013 Importantly, Cav-1 was shown to suppress hydrogen peroxide and hydroxyl radical formation by sustaining the level of activated Akt which was critical for the role of Cav-1 in attenuating the cell adhesion. Hydrogen Peroxide 41-58 AKT serine/threonine kinase 1 Homo sapiens 127-130 22566145-0 2012 Phytosphingosine-1-phosphate represses the hydrogen peroxide-induced activation of c-Jun N-terminal kinase in human dermal fibroblasts through the phosphatidylinositol 3-kinase/Akt pathway. Hydrogen Peroxide 43-60 AKT serine/threonine kinase 1 Homo sapiens 177-180 22566145-6 2012 Interestingly, regulation of the JNK/Akt pathway by PhS1P attenuated H(2)O(2)-induced cell growth arrest. Hydrogen Peroxide 69-77 AKT serine/threonine kinase 1 Homo sapiens 37-40 22797316-7 2012 Moreover, the phosphorylation of Akt was more susceptible to H2O2 insult than IGF-1 receptors. Hydrogen Peroxide 61-65 AKT serine/threonine kinase 1 Homo sapiens 33-36 22763095-0 2012 Sphingosine-1-phosphate inhibits H2O2-induced granulosa cell apoptosis via the PI3K/Akt signaling pathway. Hydrogen Peroxide 33-37 AKT serine/threonine kinase 1 Homo sapiens 84-87 22507555-7 2012 Melatonin blocked H(2) O(2) -induced phosphorylation of PI3K/Akt, p38, ERK, JNK, and MAPK, as well as activation of NF-kappaB, which was reversed by sirtinol and SIRT1 siRNA. Hydrogen Peroxide 18-27 AKT serine/threonine kinase 1 Homo sapiens 61-64 22492997-7 2012 Additionally, knockdown of Trx1 significantly abrogates DJ-1-dependent, hydrogen peroxide-induced activation of the pro-survival factor AKT. Hydrogen Peroxide 72-89 AKT serine/threonine kinase 1 Homo sapiens 136-139 22580126-0 2012 Inhibition of hydrogen peroxide signaling by 4-hydroxynonenal due to differential regulation of Akt1 and Akt2 contributes to decreases in cell survival and proliferation in hepatocellular carcinoma cells. Hydrogen Peroxide 14-31 AKT serine/threonine kinase 1 Homo sapiens 96-100 22580126-5 2012 We demonstrate that 4-HNE inhibits hydrogen peroxide-mediated phosphorylation of Akt1 but not Akt2. Hydrogen Peroxide 35-52 AKT serine/threonine kinase 1 Homo sapiens 81-85 22580126-6 2012 Pretreatment of HepG2 cells with 4-HNE prevented hydrogen peroxide stimulation of Akt-dependent phosphorylation of downstream targets and intracellular Akt activity compared with untreated control cells. Hydrogen Peroxide 49-66 AKT serine/threonine kinase 1 Homo sapiens 82-85 22580126-6 2012 Pretreatment of HepG2 cells with 4-HNE prevented hydrogen peroxide stimulation of Akt-dependent phosphorylation of downstream targets and intracellular Akt activity compared with untreated control cells. Hydrogen Peroxide 49-66 AKT serine/threonine kinase 1 Homo sapiens 152-155 22796327-10 2012 A significant increase in Akt activation was observed in proline-treated cells after hydrogen peroxide stress along with a corresponding increase in the phosphorylation of the forkhead transcription factor class O3a (FoxO3a). Hydrogen Peroxide 85-102 AKT serine/threonine kinase 1 Homo sapiens 26-29 22420665-0 2012 Luteolin inhibited hydrogen peroxide-induced vascular smooth muscle cells proliferation and migration by suppressing the Src and Akt signalling pathways. Hydrogen Peroxide 19-36 AKT serine/threonine kinase 1 Homo sapiens 129-132 22420665-7 2012 In previous research, we originally explored the function of luteolin in blocking H(2) O(2) -triggered Src and Akt signalling pathways. Hydrogen Peroxide 82-91 AKT serine/threonine kinase 1 Homo sapiens 111-114 22420665-9 2012 CONCLUSIONS: These findings strongly suggested that luteolin suppresses H(2) O(2) -directed migration and proliferation in VSMCs partially due to down-regulation of the Akt and Src signalling pathways, which are important participants in the processes of migration and proliferation of VSMCs. Hydrogen Peroxide 72-81 AKT serine/threonine kinase 1 Homo sapiens 169-172 21979951-5 2011 Overexpression of GAPDH increased ovarian cancer cell apoptosis induced by H(2)O(2), which was inhibited by Akt2 overexpression and restored by the PI3K/Akt inhibitor wortmannin or Akt2 siRNA. Hydrogen Peroxide 75-83 AKT serine/threonine kinase 1 Homo sapiens 108-111 21742779-12 2011 In conclusion, exogenous H(2)O(2) triggers two parallel independent pathways, one leading to autophagy and autophagy-dependent apoptosis, the other to transient Akt phosphorylation, and both are inhibited by DFO. Hydrogen Peroxide 25-33 AKT serine/threonine kinase 1 Homo sapiens 161-164 21643999-11 2011 Application of the Akt-inhibitor Wortmanin reveals only a partial inhibition of PAP I-dependent protection of CGN from H(2)O(2)-induced oxidative stress. Hydrogen Peroxide 119-127 AKT serine/threonine kinase 1 Homo sapiens 19-22 21315693-0 2011 Preventing H2O2-induced apoptosis in cerebellar granule neurons by regulating the VEGFR-2/Akt signaling pathway using a novel dimeric antiacetylcholinesterase bis(12)-hupyridone. Hydrogen Peroxide 11-15 AKT serine/threonine kinase 1 Homo sapiens 90-93 21315693-7 2011 These findings strongly suggest that B12H prevents H2O2-induced neuronal apoptosis independent of inhibiting AChE, but through regulating VEGFR-2/Akt/GSK3beta signaling pathway. Hydrogen Peroxide 51-55 AKT serine/threonine kinase 1 Homo sapiens 146-149 21418589-6 2011 The low H2O2 levels protected human LO2 hepatocytes from sTRAIL:FeSOD-induced apoptosis despite downregulation of p-Akt. Hydrogen Peroxide 8-12 AKT serine/threonine kinase 1 Homo sapiens 116-119 21300705-7 2011 Hydrogen peroxide (H(2)O(2)) or cigarette smoke extract (CSE) increased the level of phosphorylated Akt (pAkt) and reduced HDAC activity. Hydrogen Peroxide 0-17 AKT serine/threonine kinase 1 Homo sapiens 100-103 21237235-1 2011 against hydrogen peroxide-induced apoptosis via phosphorylation of MAPKs and PI3K/Akt in SH-SY5Y cells. Hydrogen Peroxide 8-25 AKT serine/threonine kinase 1 Homo sapiens 82-85 21237235-9 2011 Furthermore, LJ significantly attenuated the H(2)O(2)-induced phosphorylation of Akt, JNK, p38 MAPK, and ERK1/2. Hydrogen Peroxide 45-53 AKT serine/threonine kinase 1 Homo sapiens 81-84 21467197-6 2011 Both ERK and p38MAPK were activated (peaked at 8 h in ERK and 1 h in p38MAPK) by H2O2 treatment, whereas c-Jun N-terminal kinase and Akt were not. Hydrogen Peroxide 81-85 AKT serine/threonine kinase 1 Homo sapiens 133-136 21452000-9 2011 In vitro, H2O2 treatment induced up-regulation of VEGF at both the mRNA and protein levels, activated Akt, and resulted in the proliferation of HUVECs; the addition of wortmannin and NAC counteracted the effects of OS. Hydrogen Peroxide 10-14 AKT serine/threonine kinase 1 Homo sapiens 102-105 20965163-0 2011 Honokiol inhibits H(2)O(2)-induced apoptosis in human lens epithelial cells via inhibition of the mitogen-activated protein kinase and Akt pathways. Hydrogen Peroxide 18-26 AKT serine/threonine kinase 1 Homo sapiens 135-138 21397862-6 2011 A high ratio of O(2)(-):H(2)O(2) oxidizes/activates Src, triggering the PI3K/PKBalpha and ERK prosurvival pathways to confer anoikis resistance, thus promoting tumor growth. Hydrogen Peroxide 24-32 AKT serine/threonine kinase 1 Homo sapiens 77-85 21239473-7 2011 Mechanistic investigations revealed that LOX activated the PI3K (phosphoinositide 3-kinase)-Akt signaling pathway, thereby upregulating HIF-1alpha protein synthesis in a manner requiring LOX-mediated hydrogen peroxide production. Hydrogen Peroxide 200-217 AKT serine/threonine kinase 1 Homo sapiens 92-95 20965163-5 2011 Mechanistically, honokiol suppressed H(2)O(2)-induced phosphorylation of ERK1/2, p38 mitogen-activated protein kinase (MAPK), JNK and Akt. Hydrogen Peroxide 37-45 AKT serine/threonine kinase 1 Homo sapiens 134-137 20965163-7 2011 These results demonstrate that honokiol suppresses H(2)O(2)-induced HLE cell apoptosis via interference with the MAPKs, Akt and NF-kappaB signaling, suggesting that honokiol might have a potential effect against cataract formation. Hydrogen Peroxide 51-59 AKT serine/threonine kinase 1 Homo sapiens 120-123 20142804-6 2010 Furthermore, we identified that H(2)O(2) induction of ROS inhibited the upstream kinases, Akt and phosphoinositide-dependent kinase 1 (PDK1), but not the type I insulin-like growth factor receptor (IGFR), and activated the negative regulator, AMP-activated protein kinase alpha (AMPKalpha), but not the phosphatase and tensin homolog (PTEN) in the cells. Hydrogen Peroxide 32-40 AKT serine/threonine kinase 1 Homo sapiens 90-93 21215240-0 2010 Protective effect of H2O2 against subsequent H2O2-induced cytotoxicity involves activation of the PI3K-Akt signaling pathway. Hydrogen Peroxide 21-25 AKT serine/threonine kinase 1 Homo sapiens 103-106 21215240-0 2010 Protective effect of H2O2 against subsequent H2O2-induced cytotoxicity involves activation of the PI3K-Akt signaling pathway. Hydrogen Peroxide 45-49 AKT serine/threonine kinase 1 Homo sapiens 103-106 21215240-4 2010 The phosphorylation of Akt/PKB, a downstream target of phosphatydylinositol-3 kinase (PI3K), at Ser473 was augmented by H2O2 (10 muM) administration. Hydrogen Peroxide 120-124 AKT serine/threonine kinase 1 Homo sapiens 23-30 21215240-7 2010 In addition, pretreatment with LY294002 reduced H2O2 (10 muM, 10 min)-induced phosphorylation of Akt at Ser473. Hydrogen Peroxide 48-52 AKT serine/threonine kinase 1 Homo sapiens 97-100 21215240-8 2010 These findings suggest that a sublethal concentration of H2O2 exerts a cytoprotective effect against subsequent H2O2-induced cell death and that this cytoprotective effect of H2O2 is mediated by activation of the PI3K-Akt signaling pathway. Hydrogen Peroxide 57-61 AKT serine/threonine kinase 1 Homo sapiens 218-221 21215240-8 2010 These findings suggest that a sublethal concentration of H2O2 exerts a cytoprotective effect against subsequent H2O2-induced cell death and that this cytoprotective effect of H2O2 is mediated by activation of the PI3K-Akt signaling pathway. Hydrogen Peroxide 112-116 AKT serine/threonine kinase 1 Homo sapiens 218-221 21215240-8 2010 These findings suggest that a sublethal concentration of H2O2 exerts a cytoprotective effect against subsequent H2O2-induced cell death and that this cytoprotective effect of H2O2 is mediated by activation of the PI3K-Akt signaling pathway. Hydrogen Peroxide 112-116 AKT serine/threonine kinase 1 Homo sapiens 218-221 21103079-9 2010 We also found that hydrogen peroxide and delphinidin affected PI3K/Akt/mTOR signaling pathway which is one of upstream regulators of HIFs. Hydrogen Peroxide 19-36 AKT serine/threonine kinase 1 Homo sapiens 67-70 21103079-10 2010 In conclusion, hydrogen peroxide and antioxidant delphinidin seem to regulate intracellular level of p27 through regulating HIF-1 level which is, in turn, governed by its upstream regulators comprising of PI3K/Akt/mTOR signaling pathway. Hydrogen Peroxide 15-32 AKT serine/threonine kinase 1 Homo sapiens 210-213 22102892-4 2011 Treatment with high concentrations of H2O2 (25-50 microM) for 3 h reduced insulin-stimulated Akt phosphorylation, and increased the phosphorylation of both JNK and its substrate c-Jun. Hydrogen Peroxide 38-42 AKT serine/threonine kinase 1 Homo sapiens 93-96 22102892-5 2011 In contrast, lower concentrations of H2O2 (5-10 microM) enhanced insulin-stimulated phosphorylation of Akt. Hydrogen Peroxide 37-41 AKT serine/threonine kinase 1 Homo sapiens 103-106 20145959-7 2010 H2O2 reduced the activity of extracellular signal-regulated kinases Erk-1/2 and Akt, signalling proteins involved in proliferation/survival pathways. Hydrogen Peroxide 0-4 AKT serine/threonine kinase 1 Homo sapiens 80-83 20211239-9 2010 In addition, Akt1 was also found in the mitochondria and H(2)O(2) treatment led to reduced active Akt1 in these organelles, suggesting that similar regulatory mechanisms operate in mitochondria and the nucleus. Hydrogen Peroxide 57-65 AKT serine/threonine kinase 1 Homo sapiens 98-102 19664007-10 2009 CONCLUSIONS: These findings suggest that in human airway epithelium, L-carbocisteine may inhibit cell damage induced by H(2)O(2) through the activation of Akt phosphorylation. Hydrogen Peroxide 120-128 AKT serine/threonine kinase 1 Homo sapiens 155-158 19631197-7 2009 Butin enhanced the expression of phosphorylated Akt (active form of Akt), a regulator of OGG1, which was decreased by H2O2 treatment. Hydrogen Peroxide 118-122 AKT serine/threonine kinase 1 Homo sapiens 48-51 19631197-7 2009 Butin enhanced the expression of phosphorylated Akt (active form of Akt), a regulator of OGG1, which was decreased by H2O2 treatment. Hydrogen Peroxide 118-122 AKT serine/threonine kinase 1 Homo sapiens 68-71 19962976-0 2010 Catalpol inhibits apoptosis in hydrogen peroxide-induced endothelium by activating the PI3K/Akt signaling pathway and modulating expression of Bcl-2 and Bax. Hydrogen Peroxide 31-48 AKT serine/threonine kinase 1 Homo sapiens 92-95 19962976-11 2010 Taken together, these results suggest that pre-treatment of HUVECs with catalpol can block H(2)O(2)-induced apoptosis, and that the underlying mechanism involves reactive oxygen species scavenging, activation of the PI3K/Akt-Bad signaling pathway and increased Bcl-2 and decreased Bax expression. Hydrogen Peroxide 91-99 AKT serine/threonine kinase 1 Homo sapiens 221-224 20133695-6 2010 We provide evidence that DJ-1 promotes AKT phosphorylation in response to oxidative stress induced by H(2)O(2) in vitro and in vivo following 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) treatment. Hydrogen Peroxide 102-110 AKT serine/threonine kinase 1 Homo sapiens 39-42 19414004-3 2009 It was found that H(2)O(2)-induced apoptotic cell death in SH-SY5Y cells, which was revealed in DNA fragmentation, mitochondrial membrane potential depolarization, and activation of Akt, c-Jun N-terminal kinases (JNK) and caspase 3. Hydrogen Peroxide 18-26 AKT serine/threonine kinase 1 Homo sapiens 182-185 19414004-4 2009 Low doses of EGb761 (50-100 microg/ml) inhibited H(2)O(2)-induced cell apoptosis via inactivation of Akt, JNK and caspase 3 while high doses of EGb761 (250-500 microg/ml) enhanced H(2)O(2) toxicities via inactivation of Akt and enhancement of activation of JNK and caspase 3. Hydrogen Peroxide 49-57 AKT serine/threonine kinase 1 Homo sapiens 101-104 19508320-6 2009 Phosphorylation state of the kinases Akt, GSK-3beta, mTOR and ERK1/ERK2 of H(2)O(2)-treated HK-2 cells was slightly altered in the presence of erythropoietin at concentration of 100 U/ml, but was significantly less in the presence of erythropoietin at a concentration of 400 U/ml. Hydrogen Peroxide 75-83 AKT serine/threonine kinase 1 Homo sapiens 37-40 19451193-5 2009 Here, we show that H(2)O(2)-induced autophagy and apoptosis in U251 cells are mediated through the Beclin 1 and Akt/mTOR pathways. Hydrogen Peroxide 19-27 AKT serine/threonine kinase 1 Homo sapiens 112-115 19451193-9 2009 Our data provide the first evidence that H(2)O(2) induces autophagy through interference with the Beclin 1 and Akt/mTOR signaling pathways and is regulated by the anti-apoptotic gene Bcl-2 in glioma U251 cells. Hydrogen Peroxide 41-49 AKT serine/threonine kinase 1 Homo sapiens 111-114 19278624-0 2009 Hydrogen peroxide-induced Akt phosphorylation regulates Bax activation. Hydrogen Peroxide 0-17 AKT serine/threonine kinase 1 Homo sapiens 26-29 19278624-2 2009 H(2)O(2), acting as an intracellular messenger, activates phosphatidylinositol-3 kinase (PI3K) and its downstream target Akt, and promotes cell survival. Hydrogen Peroxide 0-8 AKT serine/threonine kinase 1 Homo sapiens 121-124 19278624-8 2009 Collectively, these findings reveal a mechanism by which H(2)O(2)-induced activation of PI3K/Akt influences post-translational modification of Bax and inactivates a key component of the cell death machinery. Hydrogen Peroxide 57-65 AKT serine/threonine kinase 1 Homo sapiens 93-96 18931031-0 2008 Akt activates NOS3 and separately restores barrier integrity in H2O2-stressed human cardiac microvascular endothelium. Hydrogen Peroxide 64-68 AKT serine/threonine kinase 1 Homo sapiens 0-3 19254950-2 2009 In permanently transfected cells with approximate 2-fold overexpression of GPx-1, we found that intracellular accumulation of oxidants in response to exogenous hydrogen peroxide was diminished, as was epidermal growth factor receptor (EGFR)-mediated Akt activation in response to hydrogen peroxide or EGF stimulation. Hydrogen Peroxide 160-177 AKT serine/threonine kinase 1 Homo sapiens 250-253 18841465-11 2009 These results suggest that ugonin K by activation of ERK1/2 and PI3K/Akt signal pathways protects SH-SY5Y cells from H(2)O(2)-induced apoptosis. Hydrogen Peroxide 117-125 AKT serine/threonine kinase 1 Homo sapiens 69-72 19010391-0 2009 p62 protects SH-SY5Y neuroblastoma cells against H2O2-induced injury through the PDK1/Akt pathway. Hydrogen Peroxide 49-53 AKT serine/threonine kinase 1 Homo sapiens 86-89 19010391-6 2009 p62 expression prolonged Akt phosphorylation during the later stages of H(2)O(2)-induced cell death. Hydrogen Peroxide 72-80 AKT serine/threonine kinase 1 Homo sapiens 25-28 18931031-5 2008 HCMVEC exposed to 500 microM H2O2 had increased Akt phosphorylation within 10 min at both Ser-473 and Thr-308 sites, an effect blocked by the phosphatidylinositol 3-kinase inhibitor LY-294002. Hydrogen Peroxide 29-33 AKT serine/threonine kinase 1 Homo sapiens 48-51 18931031-6 2008 H2O2 also induced NO generation that was associated with NOS3 Ser-1177 site phosphorylation and Thr-495 dephosphorylation, with Ser-1177 effects attenuated by LY-294002 and an Akt inhibitor, Akt/PKB signaling inhibitor-2 (API-2). Hydrogen Peroxide 0-4 AKT serine/threonine kinase 1 Homo sapiens 176-179 19005408-5 2008 Furthermore, H2O2-induced VCAM-1 expression was measured in beta-cells, pretreated with inhibitors of protein kinase C, phospholipase D, and phosphatidylinositol-3 kinase/Akt. Hydrogen Peroxide 13-17 AKT serine/threonine kinase 1 Homo sapiens 171-174 18425770-7 2008 The locus of fragility of these pathways is AKT, which is regulated by a balance of catalase/H2O2 or by AKT inhibitor. Hydrogen Peroxide 93-97 AKT serine/threonine kinase 1 Homo sapiens 44-47 18762777-10 2008 Kallistatin promoted survival of cardiomyocytes subjected to H(2)O(2) treatment, and inhibited H(2)O(2)-induced Akt and ERK phosphorylation, as well as NF-kappaB activation. Hydrogen Peroxide 95-103 AKT serine/threonine kinase 1 Homo sapiens 112-115 18617528-2 2008 Reactive oxygen species such as H(2)O(2) was reported to activate Akt, leading to increased eNOS Ser(1179) phosphorylation and activity. Hydrogen Peroxide 32-40 AKT serine/threonine kinase 1 Homo sapiens 66-69 18617528-8 2008 Parallel to the alterations of eNOS Ser(1179) phosphorylation, Akt was transiently activated by H(2)O(2) and subsequently became dormant. Hydrogen Peroxide 96-104 AKT serine/threonine kinase 1 Homo sapiens 63-66 18617528-11 2008 In long term H(2)O(2)-treated cells where Akt was deactivated, significant amounts of Ser(1179)-phosphorylated eNOS remained. Hydrogen Peroxide 13-21 AKT serine/threonine kinase 1 Homo sapiens 42-45 18617528-13 2008 Taken together, these studies revealed that Akt and AMPK orchestrated a bidirectional action on eNOS Ser(1179) phosphorylation in H(2)O(2)-treated cells. Hydrogen Peroxide 130-138 AKT serine/threonine kinase 1 Homo sapiens 44-47 18425770-8 2008 Tiny and trivial perturbations such as change in redox state in the cells by antioxidant enzyme catalase, scavenging H2O2 signaling molecule, regulates robust signaling molecule AKT, abolishing its phosporilation and inducing cascading failure of robust signaling pathways for cell growth, proliferation, migration, and angiogenesis. Hydrogen Peroxide 117-121 AKT serine/threonine kinase 1 Homo sapiens 178-181 18425770-9 2008 An anticancer effect of the antioxidant is achieved through the AKT locus, by abolishing signals from growth factors VEGF, HGF, HIF-1alpha and H2O2. Hydrogen Peroxide 143-147 AKT serine/threonine kinase 1 Homo sapiens 64-67 18005231-4 2008 In N2A neuroblasts, serum and H2O2 exhibited different kinetics of regulation for the Ser/Thr kinases Akt and glycogen synthase kinase 3beta (GSK-3beta) and for the transcription factor Nrf2, which governs redox homeostasis. Hydrogen Peroxide 30-34 AKT serine/threonine kinase 1 Homo sapiens 102-105 18463227-4 2008 Thus, H(2)O(2) treatment induces a "lag phase" where signaling changes occur, including PKC activation, Akt (PKB) downregulation, activation of JNK, and downregulation of AMP-activated kinase (AMPK). Hydrogen Peroxide 6-14 AKT serine/threonine kinase 1 Homo sapiens 104-107 18463227-4 2008 Thus, H(2)O(2) treatment induces a "lag phase" where signaling changes occur, including PKC activation, Akt (PKB) downregulation, activation of JNK, and downregulation of AMP-activated kinase (AMPK). Hydrogen Peroxide 6-14 AKT serine/threonine kinase 1 Homo sapiens 109-112 18163380-9 2008 Stimulation with H2O2 also activated Akt and the inhibition of PI3-K/Akt prevented GLUT4 translocation to the same extent as with AMPK inhibition. Hydrogen Peroxide 17-21 AKT serine/threonine kinase 1 Homo sapiens 37-40 18163380-9 2008 Stimulation with H2O2 also activated Akt and the inhibition of PI3-K/Akt prevented GLUT4 translocation to the same extent as with AMPK inhibition. Hydrogen Peroxide 17-21 AKT serine/threonine kinase 1 Homo sapiens 69-72 18378684-10 2008 Inhibition of H2O2-activated AKT signaling blocked VSMC calcification and Runx2 induction concurrently. Hydrogen Peroxide 14-18 AKT serine/threonine kinase 1 Homo sapiens 29-32 18702304-7 2008 We also investigated the role of reactive oxygen species (ROS) involved in HIF-1 and VEGF expression Exogenous addition of H2O2 was sufficient to activate Akt and ERK, scavengers of H2O2 significantly inhibited hypoxia-induced Akt and ERK, and subsequent HIF-lax expression and transcriptional activity. Hydrogen Peroxide 123-127 AKT serine/threonine kinase 1 Homo sapiens 155-158 18702304-7 2008 We also investigated the role of reactive oxygen species (ROS) involved in HIF-1 and VEGF expression Exogenous addition of H2O2 was sufficient to activate Akt and ERK, scavengers of H2O2 significantly inhibited hypoxia-induced Akt and ERK, and subsequent HIF-lax expression and transcriptional activity. Hydrogen Peroxide 123-127 AKT serine/threonine kinase 1 Homo sapiens 227-230 18702304-7 2008 We also investigated the role of reactive oxygen species (ROS) involved in HIF-1 and VEGF expression Exogenous addition of H2O2 was sufficient to activate Akt and ERK, scavengers of H2O2 significantly inhibited hypoxia-induced Akt and ERK, and subsequent HIF-lax expression and transcriptional activity. Hydrogen Peroxide 182-186 AKT serine/threonine kinase 1 Homo sapiens 155-158 18702304-7 2008 We also investigated the role of reactive oxygen species (ROS) involved in HIF-1 and VEGF expression Exogenous addition of H2O2 was sufficient to activate Akt and ERK, scavengers of H2O2 significantly inhibited hypoxia-induced Akt and ERK, and subsequent HIF-lax expression and transcriptional activity. Hydrogen Peroxide 182-186 AKT serine/threonine kinase 1 Homo sapiens 227-230 18005231-5 2008 Thus, H2O2 rapidly activated Akt, inhibited GSK-3beta, and directed the transcription factor Nrf2 to the nucleus, but after 4 h Akt was inactive, GSK-3beta was active and Nrf2 was more cytosolic than nuclear. Hydrogen Peroxide 6-10 AKT serine/threonine kinase 1 Homo sapiens 29-32 18005231-6 2008 Inhibition of the PI3K/Akt pathway by LY294002, impeded the short-term effect of H2O2 on nuclear translocation of Nrf2. Hydrogen Peroxide 81-85 AKT serine/threonine kinase 1 Homo sapiens 23-26 17898383-15 2007 CONCLUSIONS: Propofol 50 microM can reduce H2O2-induced damage and apoptosis in endothelial cells, by suppressing caspase-3 activity and by increasing eNOS expression via an Akt-independent mechanism. Hydrogen Peroxide 43-47 AKT serine/threonine kinase 1 Homo sapiens 174-177 18086956-11 2008 Indeed, in vascular smooth muscle cells, the activation of the redox-sensitive kinases p38-mitogen-activate protein kinase, Akt, and extracellular signal-regulated kinase 1/2 by hydrogen peroxide and by the intracellular radical generator menadione was prevented in the presence of apocynin. Hydrogen Peroxide 178-195 AKT serine/threonine kinase 1 Homo sapiens 124-127 17115933-1 2007 In leukemic cells, glucose transport is activated by SCF and H2O2 through a common signal cascade involving Akt, PLCgamma, Syk, and the Src family, in this order. Hydrogen Peroxide 61-65 AKT serine/threonine kinase 1 Homo sapiens 108-111 17387468-7 2007 Low-dose H(2)O(2) stimulated Akt phosphorylation, implying that Akt activation in HepG2 cells is redox-sensitive. Hydrogen Peroxide 9-17 AKT serine/threonine kinase 1 Homo sapiens 29-32 17387468-7 2007 Low-dose H(2)O(2) stimulated Akt phosphorylation, implying that Akt activation in HepG2 cells is redox-sensitive. Hydrogen Peroxide 9-17 AKT serine/threonine kinase 1 Homo sapiens 64-67 17481595-6 2007 Akt activation was also induced by exogenous hydrogen peroxide. Hydrogen Peroxide 45-62 AKT serine/threonine kinase 1 Homo sapiens 0-3 17431186-2 2007 This system has been shown to protect cells from hydrogen peroxide-induced apoptosis by regulating the redox state of Akt. Hydrogen Peroxide 49-66 AKT serine/threonine kinase 1 Homo sapiens 118-121 17186497-8 2007 Dominant negative Akt attenuated the inhibitory effect of H(2)O(2) on expression of catalase. Hydrogen Peroxide 58-66 AKT serine/threonine kinase 1 Homo sapiens 18-21 17196679-7 2007 Pre-exposure to short interfering RNA (si RNA ) to p38MAPK resulted in a complete blockage of the NFkappaB p65 and IKK phosphorylations as well as the anti-apoptotic influence induced by a single low dose of H2O2 but produced a partial obstruction of Akt phosphorylation. Hydrogen Peroxide 208-212 AKT serine/threonine kinase 1 Homo sapiens 251-254 17196679-11 2007 We conclude that activation of p38MAPK by repetitive oxidative stress is the key event which through its command over down-stream survival elements such as Akt and NFkappaB controls the anti-apoptotic environment of the repetitively H2O2-stressed cells. Hydrogen Peroxide 233-237 AKT serine/threonine kinase 1 Homo sapiens 156-159 17003457-8 2006 The effect of H2O2 on downstream Akt targets was examined by Western blot using antibody against phosphorylated forkhead in rhabdomyosarcoma (FKHR) and phosphorylated glycogen synthase kinase (GSK)-3beta. Hydrogen Peroxide 14-18 AKT serine/threonine kinase 1 Homo sapiens 33-36 16885155-0 2007 ERK promotes hydrogen peroxide-induced apoptosis through caspase-3 activation and inhibition of Akt in renal epithelial cells. Hydrogen Peroxide 13-30 AKT serine/threonine kinase 1 Homo sapiens 96-99 16885155-3 2007 Exposure of RPTC to 1 mM H(2)O(2) resulted in apoptosis and activation of ERK1/2 and Akt. Hydrogen Peroxide 25-33 AKT serine/threonine kinase 1 Homo sapiens 85-88 16972266-9 2006 These results suggest the involvement of P2 purinoceptors-mediated PI3K/Akt signal pathway in the protective effect of ATP against H(2)O(2)-induced oxidative damage. Hydrogen Peroxide 131-139 AKT serine/threonine kinase 1 Homo sapiens 72-75 16972266-11 2006 In conclusion, ATP, in part, blocked H(2)O(2)-induced increase of p21(WAF1/Cip1) and p27(Kip1) expression through PI3K and Akt signal pathway in renal PTCs. Hydrogen Peroxide 37-45 AKT serine/threonine kinase 1 Homo sapiens 123-126 17045920-5 2006 EGF treatment increases H2O2 production, leading to activation of the AKT/p70S6K1 pathway, resulting in increased VEGF expression at the transcriptional level. Hydrogen Peroxide 24-28 AKT serine/threonine kinase 1 Homo sapiens 70-73 17045920-6 2006 The inhibition of H(2)O(2) production by catalase abolished EGF-induced AKT and p70S6K1 activation, and VEGF expression through HIF-1alpha expression. Hydrogen Peroxide 18-26 AKT serine/threonine kinase 1 Homo sapiens 72-75 17003457-9 2006 RESULTS: H2O2 induced Akt phosphorylation in a dose-dependent manner and also induced the phosphorylation of downstream effectors FKHR and GSK-3beta. Hydrogen Peroxide 9-13 AKT serine/threonine kinase 1 Homo sapiens 22-25 17003457-10 2006 LY markedly inhibited H2O2-mediated Akt phosphorylation and significantly enhanced caspase-associated and caspase-independent RPE cell death. Hydrogen Peroxide 22-26 AKT serine/threonine kinase 1 Homo sapiens 36-39 17003457-11 2006 CONCLUSIONS: A model oxidant, H2O2, induces PI3K and thereby activates Akt. Hydrogen Peroxide 30-34 AKT serine/threonine kinase 1 Homo sapiens 71-74 15993040-4 2006 H(2)O(2) also dephosphorylates Akt/PKB in a dose- and time-dependent manner. Hydrogen Peroxide 0-8 AKT serine/threonine kinase 1 Homo sapiens 31-34 16814101-2 2006 In primary proximal tubular epithelial cells exposed to hydrogen peroxide (H2O2), both the selective cPLA2 inhibitors and the cPLA2 antisense oligonucleotides significantly attenuated H2O2-induced arachidonic acid liberation and activation of p38(SAPK), ERK1/2, and Akt1. Hydrogen Peroxide 184-188 AKT serine/threonine kinase 1 Homo sapiens 266-270 16814101-6 2006 Furthermore, it seems that p38(SAPK) is upstream of ERK1/2 and Akt1, since a p38(SAPK) inhibitor SB203580 significantly blocked H2O2-induced activation of ERK1/2 and Akt1. Hydrogen Peroxide 128-132 AKT serine/threonine kinase 1 Homo sapiens 63-67 16814101-6 2006 Furthermore, it seems that p38(SAPK) is upstream of ERK1/2 and Akt1, since a p38(SAPK) inhibitor SB203580 significantly blocked H2O2-induced activation of ERK1/2 and Akt1. Hydrogen Peroxide 128-132 AKT serine/threonine kinase 1 Homo sapiens 166-170 16987006-5 2006 Exposure of the cells to hydrogen peroxide stimulates Akt/PKB activity and fibronectin synthesis. Hydrogen Peroxide 25-42 AKT serine/threonine kinase 1 Homo sapiens 58-61 16987028-2 2006 The authors previously reported the CaM-K inhibitor KN-93 inhibited hydrogen peroxide-induced phosphorylation of Akt on threonine 308 (T308). Hydrogen Peroxide 68-85 AKT serine/threonine kinase 1 Homo sapiens 113-116 16987028-4 2006 In contrast, hydrogen peroxide-induced phosphorylation of Akt on serine 473 (S473) was downregulated by both PI3K and CaM-K inhibition, indicating that hydrogen peroxideinduced phosphorylation of Akt on S473 was largely dependent on both PI3K and a CaM-K activity. Hydrogen Peroxide 13-30 AKT serine/threonine kinase 1 Homo sapiens 58-61 16987028-4 2006 In contrast, hydrogen peroxide-induced phosphorylation of Akt on serine 473 (S473) was downregulated by both PI3K and CaM-K inhibition, indicating that hydrogen peroxideinduced phosphorylation of Akt on S473 was largely dependent on both PI3K and a CaM-K activity. Hydrogen Peroxide 13-30 AKT serine/threonine kinase 1 Homo sapiens 196-199 16987028-6 2006 These data suggest that in response to hydrogen peroxide, two pathways are activated in Jurkat T lymphocytes that converge to result in the phosphorylation of Akt on S473 and T308. Hydrogen Peroxide 39-56 AKT serine/threonine kinase 1 Homo sapiens 159-162 16987028-9 2006 The other pathway mediated by hydrogen peroxide results in the phosphorylation of Akt on S473 and requires CaM-K, PI3K, and Src activity. Hydrogen Peroxide 30-47 AKT serine/threonine kinase 1 Homo sapiens 82-85 16840713-10 2006 Akt knockdown removed the protective effect of HO-1 on delta psi(m) during exposure to H2O2. Hydrogen Peroxide 87-91 AKT serine/threonine kinase 1 Homo sapiens 0-3 16840713-12 2006 Inhibition of PI3K-Akt reduced induction of HO-1 protein expression by H2O2 and blocked its anti-apoptotic effects. Hydrogen Peroxide 71-75 AKT serine/threonine kinase 1 Homo sapiens 19-22 15993040-4 2006 H(2)O(2) also dephosphorylates Akt/PKB in a dose- and time-dependent manner. Hydrogen Peroxide 0-8 AKT serine/threonine kinase 1 Homo sapiens 35-38 15993040-5 2006 Overexpression of Akt/PKB attenuates H(2)O(2)-induced dephosphorylation of GSK-3beta. Hydrogen Peroxide 37-45 AKT serine/threonine kinase 1 Homo sapiens 18-21 15993040-5 2006 Overexpression of Akt/PKB attenuates H(2)O(2)-induced dephosphorylation of GSK-3beta. Hydrogen Peroxide 37-45 AKT serine/threonine kinase 1 Homo sapiens 22-25 16461001-5 2006 The Akt inhibitor SH6 blocked Ang II-, ArAc-, or H(2)O(2)-induced Akt activation, as did inhibition of phosphoinositide 3-kinase (PI(3)K). Hydrogen Peroxide 49-57 AKT serine/threonine kinase 1 Homo sapiens 4-7 16461001-5 2006 The Akt inhibitor SH6 blocked Ang II-, ArAc-, or H(2)O(2)-induced Akt activation, as did inhibition of phosphoinositide 3-kinase (PI(3)K). Hydrogen Peroxide 49-57 AKT serine/threonine kinase 1 Homo sapiens 66-69 16000309-0 2005 Angiopoietin-1 attenuates H2O2-induced SEK1/JNK phosphorylation through the phosphatidylinositol 3-kinase/Akt pathway in vascular endothelial cells. Hydrogen Peroxide 26-30 AKT serine/threonine kinase 1 Homo sapiens 106-109 16000309-4 2005 The inhibition of H2O2-induced JNK phosphorylation was reversed by inhibitors of phosphatidylinositol (PI) 3-kinase and dominant-negative Akt, and constitutively active-Akt attenuated JNK phosphorylation without Ang1. Hydrogen Peroxide 18-22 AKT serine/threonine kinase 1 Homo sapiens 138-141 16000309-4 2005 The inhibition of H2O2-induced JNK phosphorylation was reversed by inhibitors of phosphatidylinositol (PI) 3-kinase and dominant-negative Akt, and constitutively active-Akt attenuated JNK phosphorylation without Ang1. Hydrogen Peroxide 18-22 AKT serine/threonine kinase 1 Homo sapiens 169-172 16000309-6 2005 H2O2-induced phosphorylation of SAPK/Erk kinase (SEK1) at Thr261, which is an upstream regulator of JNK, was also attenuated by Ang1-dependent activation of the PI 3-kinase/Akt pathway. Hydrogen Peroxide 0-4 AKT serine/threonine kinase 1 Homo sapiens 173-176 16000309-8 2005 These results demonstrated that Ang1 attenuates H2O2-induced SEK1/JNK phosphorylation through the PI 3-kinase/Akt pathway and inhibits the apoptosis of endothelial cells to oxidative stress. Hydrogen Peroxide 48-52 AKT serine/threonine kinase 1 Homo sapiens 110-113 16306192-9 2005 Furthermore, activation of PKC reduced the Akt phosphorylation, whereas inhibition of PKC attenuated H2O2-mediated activation of caspase-3 and enhanced the H2O2-induced Akt phosphorylation. Hydrogen Peroxide 101-105 AKT serine/threonine kinase 1 Homo sapiens 169-172 16306192-9 2005 Furthermore, activation of PKC reduced the Akt phosphorylation, whereas inhibition of PKC attenuated H2O2-mediated activation of caspase-3 and enhanced the H2O2-induced Akt phosphorylation. Hydrogen Peroxide 156-160 AKT serine/threonine kinase 1 Homo sapiens 43-46 16306192-9 2005 Furthermore, activation of PKC reduced the Akt phosphorylation, whereas inhibition of PKC attenuated H2O2-mediated activation of caspase-3 and enhanced the H2O2-induced Akt phosphorylation. Hydrogen Peroxide 156-160 AKT serine/threonine kinase 1 Homo sapiens 169-172 16174443-5 2005 The phosphorylation level of Akt decreased after serum withdrawal and treatment with the MEK inhibitor Uo126, but increased after treatment with H(2)O(2) at low concentration, whereas none of these treatments changed PRAS40 activity. Hydrogen Peroxide 145-153 AKT serine/threonine kinase 1 Homo sapiens 29-32 15534200-3 2004 With the use of cells overexpressing NADPH oxidase 1 or peroxiredoxin II, we have now shown that H2O2 produced in response to stimulation of cells with epidermal growth factor or platelet-derived growth factor potentiates PIP3 generation and activation of the protein kinase Akt induced by these growth factors. Hydrogen Peroxide 97-101 AKT serine/threonine kinase 1 Homo sapiens 275-278 15782121-7 2005 However, Akt is released from the N-terminal domain concomitant with binding to the C-terminal domain of ASK1 in response to ASK1 activator H(2)O(2), inhibitor of Hsp90 17-AAG and Akt inhibitor LY294002, leading to a more stable Hsp90-Akt-ASK1 complex. Hydrogen Peroxide 140-148 AKT serine/threonine kinase 1 Homo sapiens 9-12 15782127-5 2005 Furthermore, in U937 cells, the hydrogen peroxide scavenger catalase and a superoxide dismutase (SOD) mimetic, TBAP, blocked these events, as well as Akt inactivation. Hydrogen Peroxide 32-49 AKT serine/threonine kinase 1 Homo sapiens 150-153 15808421-2 2005 Recent reports show that H(2)O(2) induces phosphorylation and activation of endothelial nitric oxide synthase (eNOS) through an Akt-phosphorylation-dependent pathway. Hydrogen Peroxide 25-33 AKT serine/threonine kinase 1 Homo sapiens 128-131 15808421-4 2005 Whereas moderate levels of H(2)O(2) (100 microM) activated the Akt/eNOS pathway, higher levels (500 microM) did not, suggesting differential effects by differing levels of oxidative stress. Hydrogen Peroxide 27-35 AKT serine/threonine kinase 1 Homo sapiens 63-66 15808421-7 2005 However, down-regulation of cell membrane surface and intracellular free thiols was associated with the inhibition of phosphorylation, suggesting that oxidation of non-GSH thiols inhibits the H(2)O(2)-induced phosphorylation of eNOS and Akt. Hydrogen Peroxide 192-200 AKT serine/threonine kinase 1 Homo sapiens 237-240 15475010-7 2004 Therefore, our results suggest that the increased susceptibility of Gpx1-/- cells to H2O2-induced apoptosis can be attributed in part to diminished activation of Akt despite an up-regulation in the activation of the prosurvival NFkappaB. Hydrogen Peroxide 85-89 AKT serine/threonine kinase 1 Homo sapiens 162-165 15251448-2 2004 Our data indicated that H2O2 induced acute cell apoptosis in NS/PC in concentration- and time-dependent manners and selectively, it transiently increased PI3K-Akt and Mek-Erk1/2 in a dose-dependent manner. Hydrogen Peroxide 24-28 AKT serine/threonine kinase 1 Homo sapiens 159-162 15180991-2 2004 We found that inhibition of flavin- or heme-containing proteins eliminated H(2)O(2)-induced transactivation of the epidermal growth factor receptor and stimulation of its downstream targets, JNK and Akt. Hydrogen Peroxide 75-83 AKT serine/threonine kinase 1 Homo sapiens 199-202 15251448-3 2004 Inhibition of PI3K-Akt with wortmannin, a PI3-K inhibitor, was found to significantly increase H2O2-induced acute apoptosis and dramatically decrease basal pGSK3beta levels. Hydrogen Peroxide 95-99 AKT serine/threonine kinase 1 Homo sapiens 19-22 15251448-5 2004 We conclude that the transient activation of PI3K-Akt signaling delays the H2O2-induced acute apoptosis in cultured NS/PCs in part through maintaining the basal pGSK3beta level and activating other downstream effectors. Hydrogen Peroxide 75-79 AKT serine/threonine kinase 1 Homo sapiens 50-53 15123696-7 2004 These results show that H(2)O(2) from NAD(P)H oxidase forms GSS-Ras on Cys(118) and increases its activity leading to p38 and Akt phosphorylation, which contributes to the induction of protein synthesis. Hydrogen Peroxide 24-32 AKT serine/threonine kinase 1 Homo sapiens 126-129 14504398-3 2003 Akt deactivation occurs in multiple models of cell death including N-methyl-d-aspartate excitotoxicity, vascular stroke, and nitric oxide (NO)- and hydrogen peroxide (H2O2)-elicited death of HeLa, PC12, and Jurkat T cells. Hydrogen Peroxide 148-165 AKT serine/threonine kinase 1 Homo sapiens 0-3 15048882-0 2004 TOR kinase and Ran are downstream from PI3K/Akt in H2O2-induced mitosis. Hydrogen Peroxide 51-55 AKT serine/threonine kinase 1 Homo sapiens 44-47 15048882-1 2004 Hydrogen peroxide (H2O2) activates signaling cascades essential for cell proliferation via phosphatidylinositol-3-kinase (PI3K) and Akt. Hydrogen Peroxide 0-17 AKT serine/threonine kinase 1 Homo sapiens 132-135 15048882-1 2004 Hydrogen peroxide (H2O2) activates signaling cascades essential for cell proliferation via phosphatidylinositol-3-kinase (PI3K) and Akt. Hydrogen Peroxide 19-23 AKT serine/threonine kinase 1 Homo sapiens 132-135 15048882-2 2004 Here we show that induction of mitogenic signaling by H2O2 activates sequentially PI3K, Akt, mammalian target of rapamycin (mTOR), and Ran protein. Hydrogen Peroxide 54-58 AKT serine/threonine kinase 1 Homo sapiens 88-91 15048882-5 2004 H2O2-induced Akt ser-473 phosphorylation and upregulation of Ran protein were prevented by wortmannin but not by rapamycin, indicating that PI3K is upstream of Akt and mTOR is downstream from Akt. Hydrogen Peroxide 0-4 AKT serine/threonine kinase 1 Homo sapiens 13-16 15048882-5 2004 H2O2-induced Akt ser-473 phosphorylation and upregulation of Ran protein were prevented by wortmannin but not by rapamycin, indicating that PI3K is upstream of Akt and mTOR is downstream from Akt. Hydrogen Peroxide 0-4 AKT serine/threonine kinase 1 Homo sapiens 160-163 15048882-5 2004 H2O2-induced Akt ser-473 phosphorylation and upregulation of Ran protein were prevented by wortmannin but not by rapamycin, indicating that PI3K is upstream of Akt and mTOR is downstream from Akt. Hydrogen Peroxide 0-4 AKT serine/threonine kinase 1 Homo sapiens 160-163 15048882-6 2004 Overexpression of myr-Akt or Ran-wt in type II pneumocytes increased Akt ser-473 phosphorylation and mitosis in a catalase-dependent manner, indicating that H2O2 is essential for Akt and Ran signaling. Hydrogen Peroxide 157-161 AKT serine/threonine kinase 1 Homo sapiens 22-25 15048882-6 2004 Overexpression of myr-Akt or Ran-wt in type II pneumocytes increased Akt ser-473 phosphorylation and mitosis in a catalase-dependent manner, indicating that H2O2 is essential for Akt and Ran signaling. Hydrogen Peroxide 157-161 AKT serine/threonine kinase 1 Homo sapiens 69-72 15048882-6 2004 Overexpression of myr-Akt or Ran-wt in type II pneumocytes increased Akt ser-473 phosphorylation and mitosis in a catalase-dependent manner, indicating that H2O2 is essential for Akt and Ran signaling. Hydrogen Peroxide 157-161 AKT serine/threonine kinase 1 Homo sapiens 69-72 15048882-7 2004 These results indicate that H2O2-induced mitogenic signaling in primary type II pneumocytes is mediated by PI3K, Akt, mTOR-kinase, and Ran protein. Hydrogen Peroxide 28-32 AKT serine/threonine kinase 1 Homo sapiens 113-116 14633709-9 2003 Moreover, the direct treatment of MCF-10A cells with hydrogen peroxide enhanced EGFR, Akt, and extracellular-regulated kinase phosphorylation that could be similarly inhibited by AG1478, N-acetyl cysteine, and catalase. Hydrogen Peroxide 53-70 AKT serine/threonine kinase 1 Homo sapiens 86-89 14504398-3 2003 Akt deactivation occurs in multiple models of cell death including N-methyl-d-aspartate excitotoxicity, vascular stroke, and nitric oxide (NO)- and hydrogen peroxide (H2O2)-elicited death of HeLa, PC12, and Jurkat T cells. Hydrogen Peroxide 167-171 AKT serine/threonine kinase 1 Homo sapiens 0-3 12876303-4 2003 In this study, we examined the effects of H2O2 on Akt activation in the insulin-signaling pathway in vascular smooth muscle cells (VSMCs). Hydrogen Peroxide 42-46 AKT serine/threonine kinase 1 Homo sapiens 50-53 12899942-3 2003 TcR cross-linking of primary human T blasts and Jurkat human T cells rapidly activated the ERK, JNK, p38 and Akt kinases within minutes, and was temporally associated with TcR-stimulated production of hydrogen peroxide (H(2)O(2)). Hydrogen Peroxide 201-218 AKT serine/threonine kinase 1 Homo sapiens 109-112 12876303-6 2003 Pretreatment with H2O2 concentration- and time-dependently inhibited insulin-induced Akt phosphorylation with significant inhibition observed at 50 microM for 10 min. Hydrogen Peroxide 18-22 AKT serine/threonine kinase 1 Homo sapiens 85-88 12682076-3 2003 Exposure of cells to H2O2 rapidly increases tyrosine phosphorylation of tyrosine kinase receptors (TKRs) in the absence of growth factor binding, thus inducing the activation of downstream signaling cascades, like that of protein kinase B (AKT). Hydrogen Peroxide 21-25 AKT serine/threonine kinase 1 Homo sapiens 240-243 12063265-7 2002 Furthermore, KN-93 but not KN-92 can block hydrogen peroxide-induced Akt and IKK phosphorylation. Hydrogen Peroxide 43-60 AKT serine/threonine kinase 1 Homo sapiens 69-72 12734380-5 2003 Akt inhibitory peptide also inhibited hydrogen peroxide generation stimulated by bacterial phagocytosis. Hydrogen Peroxide 38-55 AKT serine/threonine kinase 1 Homo sapiens 0-3 12384526-6 2002 These results demonstrated that H(2)O(2), phosphatidylinositol 3-kinase, and mammalian target of rapamycin were important players for UV-induced p70(S6k) phosphorylation at Thr(389) and Thr(421)/Ser(424), whereas Akt and atypical protein kinase C were not involved in this activation. Hydrogen Peroxide 32-40 AKT serine/threonine kinase 1 Homo sapiens 213-216 12525883-0 2003 Synchronous activation of ERK 1/2, p38mapk and PKB/Akt signaling by H2O2 in vascular smooth muscle cells: potential involvement in vascular disease (review). Hydrogen Peroxide 68-72 AKT serine/threonine kinase 1 Homo sapiens 47-54 12525883-5 2003 We provide a brief overview of the effect of H2O2 on MAPKs and PKB/Akt signaling in VSMC in relation to their potential role in the pathogenesis of vascular diseases. Hydrogen Peroxide 45-49 AKT serine/threonine kinase 1 Homo sapiens 63-70 12213802-0 2002 Ceramide and reactive oxygen species generated by H2O2 induce caspase-3-independent degradation of Akt/protein kinase B. Hydrogen Peroxide 50-54 AKT serine/threonine kinase 1 Homo sapiens 99-102 12213802-1 2002 This study was designed to elucidate the mechanisms leading to down-regulation of the Akt/protein kinase B (PKB) survival pathway during H2O2-induced cell death. Hydrogen Peroxide 137-141 AKT serine/threonine kinase 1 Homo sapiens 86-89 12213802-1 2002 This study was designed to elucidate the mechanisms leading to down-regulation of the Akt/protein kinase B (PKB) survival pathway during H2O2-induced cell death. Hydrogen Peroxide 137-141 AKT serine/threonine kinase 1 Homo sapiens 108-111 12213802-2 2002 H2O2 produced early activation of Akt/PKB and also DNA damage that was followed by stabilization of p53 levels, formation of reactive oxygen species (ROS), and generation of ceramide through activation of a glutathione-sensitive neutral sphingomyelinase. Hydrogen Peroxide 0-4 AKT serine/threonine kinase 1 Homo sapiens 34-41 12213802-4 2002 A membrane-targeted active Akt version attenuated apoptosis but not necrosis induced by H2O2 and was more resistant to dephosphorylation and proteolysis induced by apoptotic concentrations of H2O2. Hydrogen Peroxide 88-92 AKT serine/threonine kinase 1 Homo sapiens 27-30 12213802-4 2002 A membrane-targeted active Akt version attenuated apoptosis but not necrosis induced by H2O2 and was more resistant to dephosphorylation and proteolysis induced by apoptotic concentrations of H2O2. Hydrogen Peroxide 192-196 AKT serine/threonine kinase 1 Homo sapiens 27-30 12213802-8 2002 Surprisingly, in caspase-3-deficient MCF-7 cells Akt was more sensitive to H2O2-induced degradation than the caspase-3 substrate poly(ADP-ribose) polymerase. Hydrogen Peroxide 75-79 AKT serine/threonine kinase 1 Homo sapiens 49-52 12054657-2 2002 In this report, we demonstrate for the first time that endogenous Bruton"s tyrosine kinase (Btk) and Akt can interact with each other in DT40 chicken B cells and human Nalm6 B cells and that this interaction is inducible following H2O2 stimulation. Hydrogen Peroxide 231-235 AKT serine/threonine kinase 1 Homo sapiens 101-104 12054657-4 2002 We have also shown the involvement of phosphatidylinositol 3-kinase (PI 3-K) and Btk in the phosphorylation of Akt following stimulation by hydrogen peroxide (H2O2). Hydrogen Peroxide 140-157 AKT serine/threonine kinase 1 Homo sapiens 111-114 12054657-4 2002 We have also shown the involvement of phosphatidylinositol 3-kinase (PI 3-K) and Btk in the phosphorylation of Akt following stimulation by hydrogen peroxide (H2O2). Hydrogen Peroxide 159-163 AKT serine/threonine kinase 1 Homo sapiens 111-114 12054657-8 2002 Taken together, based on the study of endogenous conditions, we show the novel interaction of Btk and Akt in H2O2 signaling in B cells. Hydrogen Peroxide 109-113 AKT serine/threonine kinase 1 Homo sapiens 102-105 11598110-7 2001 Interestingly, the H(2)O(2)-induced activation of Akt was entirely mediated by upstream stimulation of PI 3"-kinase activity, since treatment of 3T3-L1 adipocytes with the PI 3"-kinase inhibitors wortmannin or LY294002 completely blocked the subsequent activation of Akt by exogenous H(2)O(2). Hydrogen Peroxide 19-27 AKT serine/threonine kinase 1 Homo sapiens 50-53 11796740-0 2002 Hydrogen peroxide-mediated phosphorylation of ERK1/2, Akt/PKB and JNK in cortical neurones: dependence on Ca(2+) and PI3-kinase. Hydrogen Peroxide 0-17 AKT serine/threonine kinase 1 Homo sapiens 54-57 11796740-0 2002 Hydrogen peroxide-mediated phosphorylation of ERK1/2, Akt/PKB and JNK in cortical neurones: dependence on Ca(2+) and PI3-kinase. Hydrogen Peroxide 0-17 AKT serine/threonine kinase 1 Homo sapiens 58-61 11796740-3 2002 Using phospho-specific antibodies, a 15-min stimulation of neurones with H(2)O(2) (100 microm - 1 mm) produced a concentration-dependent phosphorylation of ERK1/2 and Akt/PKB that was partly dependent on extracellular Ca(2+) and phosphatidylinositol 3-kinase (PI3-K). Hydrogen Peroxide 73-81 AKT serine/threonine kinase 1 Homo sapiens 167-174 11796740-4 2002 Higher concentrations of H(2)O(2) (1 mm) also stimulated a phosphorylation of JNK which was totally dependent on extracellular Ca(2+) but not PI3-K. H(2)O(2)-induced phosphorylation of ERK1/2, Akt/PKB or JNK were unaffected by the NMDA channel blocker MK801. Hydrogen Peroxide 25-33 AKT serine/threonine kinase 1 Homo sapiens 193-196 11796740-4 2002 Higher concentrations of H(2)O(2) (1 mm) also stimulated a phosphorylation of JNK which was totally dependent on extracellular Ca(2+) but not PI3-K. H(2)O(2)-induced phosphorylation of ERK1/2, Akt/PKB or JNK were unaffected by the NMDA channel blocker MK801. Hydrogen Peroxide 25-33 AKT serine/threonine kinase 1 Homo sapiens 197-200 11796740-4 2002 Higher concentrations of H(2)O(2) (1 mm) also stimulated a phosphorylation of JNK which was totally dependent on extracellular Ca(2+) but not PI3-K. H(2)O(2)-induced phosphorylation of ERK1/2, Akt/PKB or JNK were unaffected by the NMDA channel blocker MK801. Hydrogen Peroxide 149-157 AKT serine/threonine kinase 1 Homo sapiens 193-196 11796740-4 2002 Higher concentrations of H(2)O(2) (1 mm) also stimulated a phosphorylation of JNK which was totally dependent on extracellular Ca(2+) but not PI3-K. H(2)O(2)-induced phosphorylation of ERK1/2, Akt/PKB or JNK were unaffected by the NMDA channel blocker MK801. Hydrogen Peroxide 149-157 AKT serine/threonine kinase 1 Homo sapiens 197-200 11958138-3 2002 Low level of exogenous ROS H2O2(1-10 mumol/L) significantly stimulated PKB activity and c-fos/c-jun expression and cell growth, which could be abolished by antioxidant danshensu (40 mg/L). Hydrogen Peroxide 27-31 AKT serine/threonine kinase 1 Homo sapiens 71-74 11795876-9 2002 Activation of Akt by hydrogen peroxide or growth factors is known to proceed via the activation growth factor receptors. Hydrogen Peroxide 21-38 AKT serine/threonine kinase 1 Homo sapiens 14-17 11795876-10 2002 In line with this, pretreatment with inhibitors of growth factor receptor tyrosine kinases blocked activation of Akt by hydrogen peroxide and growth factors, as did a src-family tyrosine kinase inhibitor or the broad-spectrum tyrosine kinase inhibitor genistein. Hydrogen Peroxide 120-137 AKT serine/threonine kinase 1 Homo sapiens 113-116 11795876-12 2002 Comparison of the time course of the oxidation of 2",7"-dichlorodihydrofluorescein in copper-treated cells with that of Akt activation led to the conclusion that production of hydroperoxides cannot be an upstream event in copper-induced Akt activation. Hydrogen Peroxide 176-190 AKT serine/threonine kinase 1 Homo sapiens 120-123 11709494-0 2002 Selenite suppresses hydrogen peroxide-induced cell apoptosis through inhibition of ASK1/JNK and activation of PI3-K/Akt pathways. Hydrogen Peroxide 20-37 AKT serine/threonine kinase 1 Homo sapiens 116-119 11709494-7 2002 However, an antiapoptotic signal molecule, Akt, was activated also by H2O2, but duration of its activation was much shorter. Hydrogen Peroxide 70-74 AKT serine/threonine kinase 1 Homo sapiens 43-46 11709494-8 2002 Selenite blocked apoptosis induced by H2O2, which was related to blocking ASK1 and further stimulating PI3-kinase/Akt activities. Hydrogen Peroxide 38-42 AKT serine/threonine kinase 1 Homo sapiens 114-117 11598110-7 2001 Interestingly, the H(2)O(2)-induced activation of Akt was entirely mediated by upstream stimulation of PI 3"-kinase activity, since treatment of 3T3-L1 adipocytes with the PI 3"-kinase inhibitors wortmannin or LY294002 completely blocked the subsequent activation of Akt by exogenous H(2)O(2). Hydrogen Peroxide 19-27 AKT serine/threonine kinase 1 Homo sapiens 267-270 11605009-3 2001 The phosphorylation of p38 started at 5 min post UV irradiation, peaked at about 30 min, and remained elevated up to 2 h. The phosphorylation of AKT started at 15 min post UV treatment, peaked at about 1 h, and remained elevated up to 2 h. We also found that H2O2 induced phosphorylation of p38 and AKT in a time- dependent manner. Hydrogen Peroxide 259-263 AKT serine/threonine kinase 1 Homo sapiens 145-148 10799549-5 2000 Expression of a dominant negative mutant of p85 (regulatory component of PI3-K) and treatment with inhibitors of PI3-K (wortmannin and LY294002) prevented H(2)O(2)-induced Akt activation. Hydrogen Peroxide 155-163 AKT serine/threonine kinase 1 Homo sapiens 172-175 10799549-9 2000 Constitutive expression of v-Akt likewise enhanced survival of H(2)O(2)-treated NIH3T3 cells. Hydrogen Peroxide 63-71 AKT serine/threonine kinase 1 Homo sapiens 29-32 10799549-10 2000 These results suggest that H(2)O(2) activates Akt via an EGFR/PI3-K-dependent pathway and that elevated Akt activity confers protection against oxidative stress-induced apoptosis. Hydrogen Peroxide 27-35 AKT serine/threonine kinase 1 Homo sapiens 46-49 10967199-4 2000 Of particular importance is hydrogen peroxide, which mediates angiotensin II stimulation of such important intracellular signals as EGF-receptor transactivation, p38 mitogen activated protein kinase, and Akt. Hydrogen Peroxide 28-45 AKT serine/threonine kinase 1 Homo sapiens 204-207