PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 30249394-5 2018 Interestingly, H2O2-treated aSyn monomer, which exhibits all-four Met residues oxidized to Met-sulfoxide, exhibited a reduced ability to form large oligomers upon treatment with DOPAL, with no effect on the population of small oligomers. Hydrogen Peroxide 15-19 synuclein alpha Homo sapiens 28-32 33809978-4 2021 Previous studies have revealed that metal-catalysed oxidation (MCO) using Cu2+ and H2O2 leads to the formation of DiY cross-links in two misfolding proteins, Abeta and alpha-synuclein, associated with AD and Parkinson"s disease respectively. Hydrogen Peroxide 83-87 synuclein alpha Homo sapiens 168-183 32905883-7 2020 In addition, disturbance of the resting glutathione redox potential following exogenous hydrogen peroxide challenge was augmented by alpha-Synuclein. Hydrogen Peroxide 88-105 synuclein alpha Homo sapiens 133-148 31735335-11 2020 Meanwhile, H2O2 stimulation upregulated PTPN2, which was reversed by NAC administration. Hydrogen Peroxide 11-15 synuclein alpha Homo sapiens 69-72 29689296-2 2018 In this study, we have examined time-dependent oxidative events related to alpha-synuclein modification using six different molar ratios of Cu2+/H2O2/protein and Cu2+/H2O2/ascorbate/protein resulting in mild to moderate extents of oxidation. Hydrogen Peroxide 145-149 synuclein alpha Homo sapiens 75-90 29922854-9 2018 In addition, NAC reversed fMLP- and H2O2-induced activation of Akt and RhoA-GTPase. Hydrogen Peroxide 36-40 synuclein alpha Homo sapiens 13-16 29689296-2 2018 In this study, we have examined time-dependent oxidative events related to alpha-synuclein modification using six different molar ratios of Cu2+/H2O2/protein and Cu2+/H2O2/ascorbate/protein resulting in mild to moderate extents of oxidation. Hydrogen Peroxide 167-171 synuclein alpha Homo sapiens 75-90 29689296-10 2018 Only low levels of intra- dityrosine cross-links and no inter- dityrosine oligomers were detected under these conditions, suggesting that ascorbate limits Cu2+/H2O2-induced alpha-synuclein modification. Hydrogen Peroxide 160-164 synuclein alpha Homo sapiens 173-188 29470695-0 2018 A banana NAC transcription factor (MusaSNAC1) impart drought tolerance by modulating stomatal closure and H2O2 content. Hydrogen Peroxide 106-110 synuclein alpha Homo sapiens 9-12 29470695-5 2018 In this study we report a banana NAC protein, MusaSNAC1 which induced stomatal closure by elevating H2O2 content in guard cells during drought stress. Hydrogen Peroxide 100-104 synuclein alpha Homo sapiens 33-36 29470695-10 2018 Results indicated an interesting mechanism of drought tolerance through stomatal closure by H2O2 generation in guard cells, regulated by a NAC-protein in banana. Hydrogen Peroxide 92-96 synuclein alpha Homo sapiens 139-142 28163159-9 2017 Under oxidative stress conditions induced by H2O2, we found that H2O2 treatment induced FtMt and alpha-synuclein expression at both the mRNA and protein levels in a dose-dependent manner. Hydrogen Peroxide 45-49 synuclein alpha Homo sapiens 97-112 29039448-0 2017 Astragaloside IV attenuates the H2O2-induced apoptosis of neuronal cells by inhibiting alpha-synuclein expression via the p38 MAPK pathway. Hydrogen Peroxide 32-36 synuclein alpha Homo sapiens 87-102 28163159-9 2017 Under oxidative stress conditions induced by H2O2, we found that H2O2 treatment induced FtMt and alpha-synuclein expression at both the mRNA and protein levels in a dose-dependent manner. Hydrogen Peroxide 65-69 synuclein alpha Homo sapiens 97-112 28163159-10 2017 FtMt overexpression protected cells against oxidative stress and alleviated the enhanced alpha-synuclein expression induced by H2O2 at the posttranscriptional level. Hydrogen Peroxide 127-131 synuclein alpha Homo sapiens 89-104 25825709-0 2015 alpha-Synuclein, a chemoattractant, directs microglial migration via H2O2-dependent Lyn phosphorylation. Hydrogen Peroxide 69-73 synuclein alpha Homo sapiens 0-15 28159635-6 2017 We provide fluorescent-protein tagging, immunofluorescence and ultrastructural evidence showing that alpha-synuclein accumulations are associated with clusters of cytoplasmic vesicles and the diameter of these vesicles increases by H2O2 in a time- and dose-dependent manner. Hydrogen Peroxide 232-236 synuclein alpha Homo sapiens 101-116 26940028-11 2016 In PD-apathy group, iron level was positively correlated with OH level (r = .011, P < .05), and H2O2 level was positively correlated with alpha-synuclein oligomer level in CSF (r = .045, P < .05). Hydrogen Peroxide 97-101 synuclein alpha Homo sapiens 139-154 25825709-5 2015 Specifically, chemotaxis occurs when alpha-syn binds to integrin CD11b, leading to H2O2 production by NADPH oxidase. Hydrogen Peroxide 83-87 synuclein alpha Homo sapiens 37-46 28164279-7 2017 Calcipotriol significantly decreased the frequency of alpha-synuclein aggregate positive cells subjected to treatments that cause raised intracellular-free Ca(II) (potassium depolarization, KCl/H2 O2 combined treatment, and rotenone) in a dose-dependent manner and increased viability. Hydrogen Peroxide 194-199 synuclein alpha Homo sapiens 54-69 23159813-8 2013 Our in vivo cell culture data shows that either transient increase in intracellular free calcium or hydrogen peroxide treatment individually were able to induce significantly (P=0.01) increased 1-4mum cytoplasmic alpha-syn aggregates after 12h in cells transiently transfected with alpha-syn-GFP. Hydrogen Peroxide 100-117 synuclein alpha Homo sapiens 213-222 24784227-4 2014 In addition, ASUV exhibited stronger anti-fibrillogenic properties than H2O2-treated AS, inhibiting the fibrillation of unmodified AS at notably low concentrations. Hydrogen Peroxide 72-76 synuclein alpha Homo sapiens 85-87 23159813-8 2013 Our in vivo cell culture data shows that either transient increase in intracellular free calcium or hydrogen peroxide treatment individually were able to induce significantly (P=0.01) increased 1-4mum cytoplasmic alpha-syn aggregates after 12h in cells transiently transfected with alpha-syn-GFP. Hydrogen Peroxide 100-117 synuclein alpha Homo sapiens 282-291 21336954-0 2011 alpha-Synuclein increases U251 cells vulnerability to hydrogen peroxide by disrupting calcium homeostasis. Hydrogen Peroxide 54-71 synuclein alpha Homo sapiens 0-15 23047606-3 2012 Our results directly show that BRCA1-null ovarian cancer cells produce large amounts of hydrogen peroxide, which can be abolished either by administration of simple antioxidants (N-acetyl-cysteine; NAC) or by replacement of the BRCA1 gene. Hydrogen Peroxide 88-105 synuclein alpha Homo sapiens 198-201 22538490-6 2012 In addition, SNCA-triplication iPSC-derived DA neurons formed early ubiquitin-positive puncta and were more sensitive to peak toxicity from hydrogen peroxide-induced stress. Hydrogen Peroxide 140-157 synuclein alpha Homo sapiens 13-17 21336954-3 2011 Overexpression of alpha-synuclein increased the basal level of [Ca(2+)]( i ), and a higher Ca(2+) response to hydrogen peroxide was further observed. Hydrogen Peroxide 110-127 synuclein alpha Homo sapiens 18-33 21336954-4 2011 The effect that alpha-synuclein overexpression caused U251 cells to be more vulnerable to hydrogen peroxide was eliminated by Ca(2+) chelator BAPTA-AM or transient receptor potential channels blocker SKF 96365 but not by L-type Ca(2+) channel blocker nimodipine. Hydrogen Peroxide 90-107 synuclein alpha Homo sapiens 16-31 23353026-10 2013 Mn-induced neurocyte damage and alpha-synuclein oligomerization were also partially alleviated by the pretreatment with GSH and aggravated by H2O2 pretreatment. Hydrogen Peroxide 142-146 synuclein alpha Homo sapiens 32-47 21658409-6 2011 After chronic treatment, wild type alpha-Syn but not the mutant variants was found to rescue cells from subsequent acute hydrogen peroxide insult. Hydrogen Peroxide 121-138 synuclein alpha Homo sapiens 35-44 21710541-3 2011 Specifically, lithium administration was found to protect against cell death in a hydrogen peroxide-treated, stable alpha-synuclein-enhanced green fluorescent protein (EGFP)-overexpressing dopaminergic N27 cell line. Hydrogen Peroxide 82-99 synuclein alpha Homo sapiens 116-131 19263279-1 2009 The D- and L-forms of N-acetylcysteine (NADC, NAC) were tested in antagonizing the toxicity mediated by hydrogen peroxide (H(2)O(2)) or tertiary butyl hydroperoxide (tBHP) in two lung cell lines to assess the effectivity of glutathione synthesis against peroxides. Hydrogen Peroxide 104-121 synuclein alpha Homo sapiens 46-49 20026206-11 2010 Finally, oxidation of methionines by H2O2 greatly inhibited alpha-Syn fibrillation in vitro, leading to the formation of relatively stable oligomers, which are not toxic to dopaminergic and GABAergic neurons. Hydrogen Peroxide 37-41 synuclein alpha Homo sapiens 60-69 19328209-5 2009 In addition, the presence of DOPAC leads to the oxidation of the methionine residues of alpha-Syn, probably due to the H(2)O(2) production as a by-product of DOPAC oxidation. Hydrogen Peroxide 119-127 synuclein alpha Homo sapiens 88-97 19263279-1 2009 The D- and L-forms of N-acetylcysteine (NADC, NAC) were tested in antagonizing the toxicity mediated by hydrogen peroxide (H(2)O(2)) or tertiary butyl hydroperoxide (tBHP) in two lung cell lines to assess the effectivity of glutathione synthesis against peroxides. Hydrogen Peroxide 123-131 synuclein alpha Homo sapiens 46-49 19263279-4 2009 H(2)O(2)-mediated toxicity was decreased by NADC (as compared to controls), but increased slightly with NAC, whereas tBHP-mediated toxicity was decreased both by NAC and NADC. Hydrogen Peroxide 0-8 synuclein alpha Homo sapiens 104-107 19263279-8 2009 Hence, it is concluded that direct antioxidative properties of NAC and NADC is a main antagonizing factor in H(2)O(2)-based toxicity but not in tBHP-mediated toxicity. Hydrogen Peroxide 109-117 synuclein alpha Homo sapiens 63-66 18239841-0 2008 Products of Cu(II)-catalyzed oxidation of alpha-synuclein fragments containing M1-D2 and H50 residues in the presence of hydrogen peroxide. Hydrogen Peroxide 121-138 synuclein alpha Homo sapiens 42-57 18986521-9 2008 In addition, H2O2 synergizes with HA fragments to induce inflammatory genes, which are inhibited by NAC. Hydrogen Peroxide 13-17 synuclein alpha Homo sapiens 100-103 18853169-8 2009 These results suggest that abnormal alpha-synuclein as found in Lewy bodies produce hydrogen peroxide and these neurons are capable of directing antioxidant enzymes to regions of oxidative stress. Hydrogen Peroxide 84-101 synuclein alpha Homo sapiens 36-51 16839607-0 2006 Products of Cu(II)-catalyzed oxidation of the N-terminal fragments of alpha-synuclein in the presence of hydrogen peroxide. Hydrogen Peroxide 105-122 synuclein alpha Homo sapiens 70-85 17337120-5 2007 Cells overexpressing wild-type alpha-synuclein were more vulnerable to hydrogen peroxide and 6-hydroxydopamine. Hydrogen Peroxide 71-88 synuclein alpha Homo sapiens 31-46 16978743-0 2007 Parkinson"s disease genetic mutations increase cell susceptibility to stress: mutant alpha-synuclein enhances H2O2- and Sin-1-induced cell death. Hydrogen Peroxide 110-114 synuclein alpha Homo sapiens 85-100 16978743-5 2007 We found that inducible expression of A30P or A53T mutant alpha-synuclein decreased the proteasome activity, increased intracellular ROS levels, and enhanced lactacystin- and H2O2-induced cell death. Hydrogen Peroxide 175-179 synuclein alpha Homo sapiens 58-73 17760417-8 2007 The released enzyme decomposed microglial hydrogen peroxide following nitrated alpha-synuclein or tumor necrosis factor alpha activation. Hydrogen Peroxide 42-59 synuclein alpha Homo sapiens 79-94 16753318-4 2007 RESULTS: Thirty minutes after nebulization, H(2)O(2) concentration increased if levels after NAC (0.45+/-0.25microM) and PL (0.17+/-0.17microM) were compared in COPD patients (p=0.002). Hydrogen Peroxide 44-52 synuclein alpha Homo sapiens 93-96 16839607-2 2006 In an attempt to elucidate the products of the copper(II)-catalyzed oxidation of the (1-17), (1-28), (1-39) and (1-39)(A30P) fragments of alpha-synuclein, the high performance liquid chromatography (HPLC) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) methods and Cu(II) /hydrogen peroxide as a model oxidizing system were employed. Hydrogen Peroxide 321-338 synuclein alpha Homo sapiens 138-153 16839607-6 2006 Reaction of hydrogen peroxide with all fragments of alpha-synuclein resulted in oxidation of two methionine residues (M(1), M(5)) to methionine sulfoxides. Hydrogen Peroxide 12-29 synuclein alpha Homo sapiens 52-67 16778450-8 2006 TSA had a more potent and longer lasting antiproliferative effect than CDB and NAC, even in the presence of an oxidant, H2O2. Hydrogen Peroxide 120-124 synuclein alpha Homo sapiens 79-82 16984000-3 2006 Hydrogen peroxide is catalytically converted to the aggressive hydroxyl radical in the presence of Fe(II) and Cu(I), which renders amyloidogenic proteins such as beta-amyloid and alpha-synuclein (implicated in Alzheimer"s disease (AD) and Parkinson"s disease (PD), respectively) vulnerable to self-inflicted hydroxyl radical attack. Hydrogen Peroxide 0-17 synuclein alpha Homo sapiens 179-194 12603836-0 2003 Lipid interaction of alpha-synuclein during the metal-catalyzed oxidation in the presence of Cu2+ and H2O2. Hydrogen Peroxide 102-106 synuclein alpha Homo sapiens 21-36 15079871-0 2004 Overexpression of alpha-synuclein decreased viability and enhanced sensitivity to prostaglandin E(2), hydrogen peroxide, and a nitric oxide donor in differentiated neuroblastoma cells. Hydrogen Peroxide 102-119 synuclein alpha Homo sapiens 18-33 15079871-3 2004 It was unknown whether neurons overexpressing alpha-synuclein would exhibit increased sensitivity to hydrogen peroxide (H(2)O(2)) or 3-morpholinosydnonimine (SIN-1; a nitrous oxide donor). Hydrogen Peroxide 101-118 synuclein alpha Homo sapiens 46-61 14742448-0 2004 Alpha-synuclein up-regulation and aggregation during MPP+-induced apoptosis in neuroblastoma cells: intermediacy of transferrin receptor iron and hydrogen peroxide. Hydrogen Peroxide 146-163 synuclein alpha Homo sapiens 0-15 14640973-8 2004 Irradiation of [(NH3)5CoCO3]+ and NO2- in the presence of alpha-synuclein yielded nitration and aggregation products that were similar to those obtained from a SIN-1 (or slowly infused ONOO-) and HCO3- or a myeloperoxidase/H2O2/NO2- system. Hydrogen Peroxide 223-227 synuclein alpha Homo sapiens 58-73 15680224-3 2005 Because it lacks Trp and Cys residues, mild oxidation of alpha-synuclein in vitro with hydrogen peroxide selectively converts all four methionine residues to the corresponding sulfoxides. Hydrogen Peroxide 87-104 synuclein alpha Homo sapiens 57-72 12807439-2 2003 The present study indicates that alpha-synuclein, but not its mutants (A53T, A30P), can protect CNS dopaminergic cells from the parkinsonism-inducing drug 1-methyl-4-phenylpyridinium (MPP+), whereas it cannot protect from the dopaminergic toxin, 6-hydroxydopamine, hydrogen-peroxide, or the beta-amyloid peptide, A-beta. Hydrogen Peroxide 265-282 synuclein alpha Homo sapiens 33-48 12603836-6 2003 In the presence of Cu2+ and H2O2, phosphatidylinositol (PI), along with other acidic lipids, actually enhanced the metal-catalyzed oxidative self-oligomerization of alpha-synuclein. Hydrogen Peroxide 28-32 synuclein alpha Homo sapiens 165-180 12661766-0 2003 Enhanced oligomerization of the alpha-synuclein mutant by the Cu,Zn-superoxide dismutase and hydrogen peroxide system. Hydrogen Peroxide 93-110 synuclein alpha Homo sapiens 32-47 12661766-4 2003 When alpha-synuclein was incubated with both Cu,Zn-SOD and H2O2, then the amount of A53T mutant oligomerization increased relative to that of the wild-type protein. Hydrogen Peroxide 59-63 synuclein alpha Homo sapiens 5-20 12031892-0 2002 Formation of hydrogen peroxide and hydroxyl radicals from A(beta) and alpha-synuclein as a possible mechanism of cell death in Alzheimer"s disease and Parkinson"s disease. Hydrogen Peroxide 13-30 synuclein alpha Homo sapiens 70-85 12453634-0 2002 The ceruloplasmin and hydrogen peroxide system induces alpha-synuclein aggregation in vitro. Hydrogen Peroxide 22-39 synuclein alpha Homo sapiens 55-70 12453634-7 2002 This indicates that the aggregation of alpha-synuclein can be mediated by the CP/H(2)O(2) system via the generation of hydroxyl radical. Hydrogen Peroxide 81-89 synuclein alpha Homo sapiens 39-54 12031892-6 2002 Our results suggest that hydrogen peroxide accumulates during the incubation of A(beta) or alpha-synuclein, by a metal-dependent mechanism, and that this is subsequently converted to hydroxyl radicals, on addition of Fe (II), by Fenton"s reaction. Hydrogen Peroxide 25-42 synuclein alpha Homo sapiens 91-106 11958955-0 2002 Aggregation of alpha-synuclein induced by the Cu,Zn-superoxide dismutase and hydrogen peroxide system. Hydrogen Peroxide 77-94 synuclein alpha Homo sapiens 15-30 11790792-4 2002 Although hydrogen peroxide challenge resulted in JNK activation and cell death in cells transfected with vector control or beta-synuclein, alpha-synuclein-transfected cells were resistant to hydrogen peroxide, and JNK was not activated. Hydrogen Peroxide 191-208 synuclein alpha Homo sapiens 139-154 10934254-4 2000 Using human BE-M17 neuroblastoma cells overexpressing wild-type, A53T, or A30P alpha-synuclein, we now show that iron and free radical generators, such as dopamine or hydrogen peroxide, stimulate the production of intracellular aggregates that contain alpha-synuclein and ubiquitin. Hydrogen Peroxide 167-184 synuclein alpha Homo sapiens 79-94 11369507-0 2001 alpha-Synuclein implicated in Parkinson"s disease catalyses the formation of hydrogen peroxide in vitro. Hydrogen Peroxide 77-94 synuclein alpha Homo sapiens 0-15 11369507-6 2001 Our new data suggest that the fundamental molecular mechanism underlying this pathological process could be the production of hydrogen peroxide by alpha-synuclein. Hydrogen Peroxide 126-143 synuclein alpha Homo sapiens 147-162 11301060-5 2001 These drugs at high concentration (3-10 mM) inhibited in vitro aggregation of cytochrome c by hydrogen peroxide or that of alpha-synuclein by cytochrome c and hydrogen peroxide. Hydrogen Peroxide 159-176 synuclein alpha Homo sapiens 123-138 11168363-9 2001 However, the metal-catalysed oxidative self-oligomerization of alpha-synuclein in the presence of Cu2+/H2O2, which was augmented synergistically by Alphabeta25-35, was not affected by the dyes. Hydrogen Peroxide 103-107 synuclein alpha Homo sapiens 63-78 10934254-4 2000 Using human BE-M17 neuroblastoma cells overexpressing wild-type, A53T, or A30P alpha-synuclein, we now show that iron and free radical generators, such as dopamine or hydrogen peroxide, stimulate the production of intracellular aggregates that contain alpha-synuclein and ubiquitin. Hydrogen Peroxide 167-184 synuclein alpha Homo sapiens 252-267 10860544-0 2000 Metal-catalyzed oxidation of alpha-synuclein in the presence of Copper(II) and hydrogen peroxide. Hydrogen Peroxide 79-96 synuclein alpha Homo sapiens 29-44 10860544-4 2000 Since oxidatively modified protein could increase its own tendency toward protein aggregation, metal-catalyzed oxidation of alpha-synuclein has been examined with copper(II) and hydrogen peroxide in the absence of the coupling reagent. Hydrogen Peroxide 178-195 synuclein alpha Homo sapiens 124-139 10860544-6 2000 This biochemically specific copper-mediated oxidative oligomerization was shown to be dependent upon the acidic C-terminus of alpha-synuclein because the C-terminally truncated proteins such as alpha-syn114 and alpha-syn97 were not affected by the metal and hydrogen peroxide. Hydrogen Peroxide 258-275 synuclein alpha Homo sapiens 126-141 10799759-4 2000 Human dopaminergic neuroblastoma SH-SY5Y cells were stably transduced with various isoforms of alpha-synuclein and their survival following exposure to hydrogen peroxide or to the dopaminergic neurotoxin MPP(+) was assessed. Hydrogen Peroxide 152-169 synuclein alpha Homo sapiens 95-110 10506125-6 1999 Hemin/hydrogen peroxide similarly induced aggregation of alpha-synuclein, and both cytochrome c/hydrogen peroxide- and hemin/hydrogen peroxide-induced aggregation of alpha-synuclein was partially inhibited by treatment with iron chelator deferoxisamine. Hydrogen Peroxide 96-113 synuclein alpha Homo sapiens 166-181 10506125-6 1999 Hemin/hydrogen peroxide similarly induced aggregation of alpha-synuclein, and both cytochrome c/hydrogen peroxide- and hemin/hydrogen peroxide-induced aggregation of alpha-synuclein was partially inhibited by treatment with iron chelator deferoxisamine. Hydrogen Peroxide 6-23 synuclein alpha Homo sapiens 57-72 10506125-6 1999 Hemin/hydrogen peroxide similarly induced aggregation of alpha-synuclein, and both cytochrome c/hydrogen peroxide- and hemin/hydrogen peroxide-induced aggregation of alpha-synuclein was partially inhibited by treatment with iron chelator deferoxisamine. Hydrogen Peroxide 96-113 synuclein alpha Homo sapiens 166-181 10506125-7 1999 This indicates that iron-catalyzed oxidative reaction mediated by cytochrome c/hydrogen peroxide might be critically involved in promoting alpha-synuclein aggregation. Hydrogen Peroxide 79-96 synuclein alpha Homo sapiens 139-154 10208537-5 1999 Ferrous ion was not effective by itself, but it potentially aggregated NACP/alpha-synuclein in the presence of hydrogen peroxide. Hydrogen Peroxide 111-128 synuclein alpha Homo sapiens 71-75